Submitted to: Journal of Virological Methods
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/27/2024
Publication Date: 9/2/2024
Citation: Nemchinov, L.G. 2024. Serological detection of a novel ipomo-like virus infecting alfalfa in the U.S. Journal of Virological Methods. 330. Article e115021. https://doi.org/10.1016/j.jviromet.2024.115021.
DOI: https://doi.org/10.1016/j.jviromet.2024.115021

Interpretive Summary: Plant viruses represent an integral part of a diverse community of pathogenic microbes infecting alfalfa. In this research, aimed at surveying alfalfa varieties grown in the U.S. to identify, characterize, and prevent the spread of novel and emerging viruses in the country, we report on the development of serological methods for specific detection of a newly discovered virus infecting alfalfa in the United States. It is expected that this research will be of interest to plant pathologists, extension specialists, alfalfa growers, and people working in the field of alfalfa improvement.

Technical Abstract: This study describes production of polyclonal antibodies against recently reported novel potyvirid infecting alfalfa (Medicago sativa L.). The virus was first found in alfalfa seed material and later identified in plant samples collected from commercial alfalfa fields in Arizona, USA. It was classified as a novel species related to the members of the genus Ipomovirus and potentially representing a new genus in the family Potyviridae (Nemchinov et al., 2023b). Polyclonal antibodies were produced against the predicted viral coat protein expressed in bacterial cells and used in different types of immunoassays for specific detection of this emerging virus. They could be helpful in plant virus certification programs, screening of alfalfa germplasm, research on pathogenicity, biology, and geographic distribution of this emerging virus.