Amir Shahein

https://www.adaptyvbio.com/blog/automancer

https://www.youtube.com/watch?v=OZv7OxHxxOs&ab_channel=AmirShahein
https://www.biorxiv.org/content/10.1101/2023.04.16.536594v1
A.S. conceived of, designed the workcell, and directed the work. A.S. and C.N. assembled and characterized the system, designed the animation, developed the workcell CAD files and manufacturing thereof. G.B. performed all experiments using the platform, and conducted wafer and chip-fabrication. A.S. developed the chip designs and CAD files for photolithography… Mehr anzeigen

https://www.youtube.com/watch?v=OZv7OxHxxOs&ab_channel=AmirShahein
https://www.biorxiv.org/content/10.1101/2023.04.16.536594v1
A.S. conceived of, designed the workcell, and directed the work. A.S. and C.N. assembled and characterized the system, designed the animation, developed the workcell CAD files and manufacturing thereof. G.B. performed all experiments using the platform, and conducted wafer and chip-fabrication. A.S. developed the chip designs and CAD files for photolithography. A.S. and G.B. wrote experiment protocols, optimized system performance and designed the automated cleaning circuit. C.N. programmed the programmable logic controller and the animation. S.L. wrote the core software application including the user-interface, and L.H. wrote the image analysis pipeline with support from A.S. A.S. wrote the paper with review from all authors. Weniger anzeigen

Contributed to a section on bottom-up engineering of complex biological systems.

A.S. performed all in vitro experiments, data analysis and modeling used in this manuscript. M.L. performed all in vivo experiments, M.L. and A.S. performed data analysis. I.I. and S.J.M. designed the initial iMITOMI method which was further refined by A.S. I.I. and S.J.M. designed the Pho4 target library. A.S. and S.J.M. designed the Zif268 target library. I.I. designed the recombinant proteins and cloned the expression plasmids. I.I. and A.S. generated the recombinant proteins. I.I. collected… Mehr anzeigen

A.S. performed all in vitro experiments, data analysis and modeling used in this manuscript. M.L. performed all in vivo experiments, M.L. and A.S. performed data analysis. I.I. and S.J.M. designed the initial iMITOMI method which was further refined by A.S. I.I. and S.J.M. designed the Pho4 target library. A.S. and S.J.M. designed the Zif268 target library. I.I. designed the recombinant proteins and cloned the expression plasmids. I.I. and A.S. generated the recombinant proteins. I.I. collected and analyzed preliminary Pho4 data. E.O. supported I.I. and A.S. with development of the iMITOMI method, modeling, and data analysis. S.C., M.L., and A.S. generated the yeast strains. A.S. and S.J.M. wrote the manuscript with input from all authors. S.J.M. conceived and directed the study. Weniger anzeigen

Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude extract systems, as well as breakthroughs in enabling technologies, that have facilitated increased throughput, compartmentalization, and spatial control of cell-free protein synthesis reactions. Combined with a deeper understanding of the… Mehr anzeigen

Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude extract systems, as well as breakthroughs in enabling technologies, that have facilitated increased throughput, compartmentalization, and spatial control of cell-free protein synthesis reactions. Combined with a deeper understanding of the cell-free systems themselves, these advances improve our ability to address a range of scientific questions. By mastering control of the cell-free platform, we will be in a position to construct increasingly complex biomolecular systems, and approach natural biological complexity in a bottom-up manner. Weniger anzeigen