Maryam Khoshouei
Principal scientist at Novartis
Schweiz
285 Follower:innen
277 Kontakte
Aktivitäten
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Thanks so much to Gebhard F. X. Schertler, Christian Rüegg and team for inviting me to speak at PSI Paul Scherrer Institut. It was a pleasure to meet…
Thanks so much to Gebhard F. X. Schertler, Christian Rüegg and team for inviting me to speak at PSI Paul Scherrer Institut. It was a pleasure to meet…
Beliebt bei Maryam Khoshouei
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My postdoc work from Novartis, finally available online.
My postdoc work from Novartis, finally available online.
Beliebt bei Maryam Khoshouei
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What a fantastic unbelievable end of my time as a volunteer at the Olympics #Paris2024 🤩 Gold medal for Triathlon mixed team Germany 🥳🥇. This was…
What a fantastic unbelievable end of my time as a volunteer at the Olympics #Paris2024 🤩 Gold medal for Triathlon mixed team Germany 🥳🥇. This was…
Beliebt bei Maryam Khoshouei
Berufserfahrung
Ausbildung
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Technische Universität München / Technical University of Munich
Doctor of Philosophy (PhD) Biochemistry/Physics
Max-Planck Institute of Biochemistry
Veröffentlichungen
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Cryo-EM structure of the active, Gs-protein complexed, human CGRP receptor.
Nature
Calcitonin gene-related peptide (CGRP) is a widely expressed neuropeptide that has a major role in sensory neurotransmission. The CGRP receptor is a heterodimer of the calcitonin receptor-like receptor (CLR) class B G-protein-coupled receptor and a type 1 transmembrane domain protein, receptor activity-modifying protein 1 (RAMP1). Here we report the structure of the human CGRP receptor in complex with CGRP and the Gs-protein heterotrimer at 3.3 Å global resolution, determined by Volta…
Calcitonin gene-related peptide (CGRP) is a widely expressed neuropeptide that has a major role in sensory neurotransmission. The CGRP receptor is a heterodimer of the calcitonin receptor-like receptor (CLR) class B G-protein-coupled receptor and a type 1 transmembrane domain protein, receptor activity-modifying protein 1 (RAMP1). Here we report the structure of the human CGRP receptor in complex with CGRP and the Gs-protein heterotrimer at 3.3 Å global resolution, determined by Volta phase-plate cryo-electron microscopy. The receptor activity-modifying protein transmembrane domain sits at the interface between transmembrane domains 3, 4 and 5 of CLR, and stabilizes CLR extracellular loop 2. RAMP1 makes only limited direct contact with CGRP, consistent with its function in allosteric modulation of CLR. Molecular dynamics simulations indicate that RAMP1 provides stability to the receptor complex, particularly in the positioning of the extracellular domain of CLR. This work provides insights into the control of G-protein-coupled receptor function.
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Structure of the adenosine-bound human adenosine A1 receptor-Gi complex.
Nature
Draper-Joyce CJ, Khoshouei M, Thal DM, Liang YL, Nguyen ATN, Furness SGB, Venugopal H, Baltos JA, Plitzko JM, Danev R, Baumeister W, May LT, Wootten D, Sexton PM, Glukhova A, Christopoulos A.
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Phase-plate cryo-EM structure of a biased agonist-bound human GLP-1 receptor–Gs complex
Nature
Authors: Yi-Lynn Liang, Maryam Khoshouei, Alisa Glukhova, Sebastian G. B. Furness, Peishen Zhao, Lachlan Clydesdale, Cassandra Koole, Tin T. Truong, David M. Thal, Saifei Lei, Mazdak Radjainia, Radostin Danev, Wolfgang Baumeister, Ming-Wei Wang, Laurence J. Miller, Arthur Christopoulos, Patrick M. Sexton & Denise Wootten
The class B glucagon-like peptide-1 (GLP-1) G protein-coupled receptor is a major target for the treatment of type 2 diabetes and obesity1. Endogenous and mimetic GLP-1…Authors: Yi-Lynn Liang, Maryam Khoshouei, Alisa Glukhova, Sebastian G. B. Furness, Peishen Zhao, Lachlan Clydesdale, Cassandra Koole, Tin T. Truong, David M. Thal, Saifei Lei, Mazdak Radjainia, Radostin Danev, Wolfgang Baumeister, Ming-Wei Wang, Laurence J. Miller, Arthur Christopoulos, Patrick M. Sexton & Denise Wootten
The class B glucagon-like peptide-1 (GLP-1) G protein-coupled receptor is a major target for the treatment of type 2 diabetes and obesity1. Endogenous and mimetic GLP-1 peptides exhibit biased agonism—a difference in functional selectivity—that may provide improved therapeutic outcomes1. Here we describe the structure of the human GLP-1 receptor in complex with the G protein-biased peptide exendin-P5 and a Gαs heterotrimer, determined at a global resolution of 3.3 Å. At the extracellular surface, the organization of extracellular loop 3 and proximal transmembrane segments differs between our exendin-P5-bound structure and previous GLP-1-bound GLP-1 receptor structure2. At the intracellular face, there was a six-degree difference in the angle of the Gαs–α5 helix engagement between structures, which was propagated across the G protein heterotrimer. In addition, the structures differed in the rate and extent of conformational reorganization of the Gαs protein. Our structure provides insights into the molecular basis of biased agonism. -
Isolation and Characterization of Metallosphaera turreted icosahedral virus (MTIV), a founding member of a new family of archaeal viruses.
Journal of Virology
Our understanding of archaeal virus diversity and structure is just beginning to emerge. Here we describe a new archaeal virus, tentatively named Metallosphaera turreted icosahedral virus (MTIV), that was isolated from an acidic hot spring in Yellowstone National Park, USA. Two strains of the virus were identified and were found to replicate in an archaeal host species closely related to Metallosphaera yellowstonensis. Each strain encodes a 9.8- to 9.9-kb linear double-stranded DNA (dsDNA)…
Our understanding of archaeal virus diversity and structure is just beginning to emerge. Here we describe a new archaeal virus, tentatively named Metallosphaera turreted icosahedral virus (MTIV), that was isolated from an acidic hot spring in Yellowstone National Park, USA. Two strains of the virus were identified and were found to replicate in an archaeal host species closely related to Metallosphaera yellowstonensis. Each strain encodes a 9.8- to 9.9-kb linear double-stranded DNA (dsDNA) genome with large inverted terminal repeats. Each genome encodes 21 open reading frames (ORFs). The ORFs display high homology between the strains, but they are quite distinct from other known viral genes. The 70-nm-diameter virion is built on a T=28 icosahedral lattice. Both single particle cryo-electron microscopy and cryotomography reconstructions reveal an unusual structure that has 42 turret-like projections: 12 pentameric turrets positioned on the icosahedral 5-fold axes and 30 turrets with apparent hexameric symmetry positioned on the icosahedral 2-fold axes. Both the virion structural properties and the genome content support MTIV as the founding member of a new family of archaeal viruses.
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Revisiting the structure of haemoglobin and myoglobin with cryo-electron microscopy
Journal of Molecular Biology (review)
Sixty years ago the first protein structure was determined by John Kendrew and his colleagues, that of myoglobin; haemoglobin followed shortly thereafter. For quite some time it seemed that only X-ray crystallography would be capable of determining the structure of proteins to high resolution. In recent years, cryo- electron microscopy has emerged as a viable alternative and indeed in many cases the preferred approach. It is capable of studying proteins which span a size range from several…
Sixty years ago the first protein structure was determined by John Kendrew and his colleagues, that of myoglobin; haemoglobin followed shortly thereafter. For quite some time it seemed that only X-ray crystallography would be capable of determining the structure of proteins to high resolution. In recent years, cryo- electron microscopy has emerged as a viable alternative and indeed in many cases the preferred approach. It is capable of studying proteins which span a size range from several megadaltons to proteins as small as myoglobin and haemoglobin.
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Cryo-EM structure of haemoglobin at 3.2 A determined with the Volta phase plate
Nature Communications
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Phase-plate cryo-EM structure of a class B GPCR–G-protein complex
Nature
Authors: Yi-Lynn Liang, Maryam Khoshouei, Mazdak Radjainia, Yan Zhang, Alisa Glukhova, Jeffrey Tarrasch, David M. Thal, Sebastian G. B. Furness, George Christopoulos, Thomas Coudrat, Radostin Danev, Wolfgang Baumeister, Laurence J. Miller, Arthur Christopoulos, Brian K. Kobilka, Denise Wootten, Georgios Skiniotis & Patrick M. Sexton
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Subtomogram analysis using the Volta phase plate.
Journal of Structural Biology
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Removing Contamination-Induced Reconstruction Artifacts from Cryo-electron Tomograms.
Biophysical journal
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Volta phase plate cryo-EM of the small protein complex Prx3.
Nature Communications
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Volta potential phase plate for in-focus phase contrast transmission electron microscopy.
Proceedings of the National Academy of Sciences of the USA(PNAS)
Andere Autor:innen
Weitere Aktivitäten von Maryam Khoshouei
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What an amazing day!!!! No words, just - wow Triathlon of women and men 🏊♂️🚴♀️🏃and I was in the middle 🤩 #Sanofistories #Paris2024
What an amazing day!!!! No words, just - wow Triathlon of women and men 🏊♂️🚴♀️🏃and I was in the middle 🤩 #Sanofistories #Paris2024
Beliebt bei Maryam Khoshouei
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My first 2 days as a Volunteer at the Olympics were fantastic 🤩 I learned that a perfect race day is all about preparation and practicing…
My first 2 days as a Volunteer at the Olympics were fantastic 🤩 I learned that a perfect race day is all about preparation and practicing…
Beliebt bei Maryam Khoshouei
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Big shoes to fill! Sandra Jacob has been leading Protein Sciences Basel at Novartis Biomedical Research for well over a decade. A widely respected…
Big shoes to fill! Sandra Jacob has been leading Protein Sciences Basel at Novartis Biomedical Research for well over a decade. A widely respected…
Beliebt bei Maryam Khoshouei
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👩🔬 Welcome NGS Interns! 🌍 I am thrilled to announce that our Next Generation Scientist (NGS) program is now in full swing, supporting talented…
👩🔬 Welcome NGS Interns! 🌍 I am thrilled to announce that our Next Generation Scientist (NGS) program is now in full swing, supporting talented…
Beliebt bei Maryam Khoshouei
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New paper out in bioarxiv! https://lnkd.in/eEKaR4HC. In collaboration with the team of Maks Ovsjanikov and Hervé Minoux head of AI at #sanofi…
New paper out in bioarxiv! https://lnkd.in/eEKaR4HC. In collaboration with the team of Maks Ovsjanikov and Hervé Minoux head of AI at #sanofi…
Beliebt bei Maryam Khoshouei
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A fantastic experience participating this year to #PEGS2024 #Boston with the exceptional team from #ThermoFisherScientific composed of Jennifer…
A fantastic experience participating this year to #PEGS2024 #Boston with the exceptional team from #ThermoFisherScientific composed of Jennifer…
Beliebt bei Maryam Khoshouei
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Our informatics team is looking for a business relationship manager. This is a very attractive position if you are excited about enabling best in…
Our informatics team is looking for a business relationship manager. This is a very attractive position if you are excited about enabling best in…
Beliebt bei Maryam Khoshouei
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Today is May the 12th. We honor the memory of Maryam Mirzakhani, the first woman to wins the highest prize in mathematics, The Fields Medal. She was…
Today is May the 12th. We honor the memory of Maryam Mirzakhani, the first woman to wins the highest prize in mathematics, The Fields Medal. She was…
Beliebt bei Maryam Khoshouei
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Congratulations to Google Deep Mind and our partners at Isomorphic Labs for the remarkable development of Alphafold 3 (https://lnkd.in/dAFCUWgm)…
Congratulations to Google Deep Mind and our partners at Isomorphic Labs for the remarkable development of Alphafold 3 (https://lnkd.in/dAFCUWgm)…
Beliebt bei Maryam Khoshouei
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#NEWS: We're excited to share our positive data from the Phase I/IIa HORNBILL study in diabetic macular ischema (DMI). #RetinalHealth…
#NEWS: We're excited to share our positive data from the Phase I/IIa HORNBILL study in diabetic macular ischema (DMI). #RetinalHealth…
Beliebt bei Maryam Khoshouei
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Thanks to Prof. Onajomo "Ona" Akemu for giving me the opportunity to guest lecture on 'AI in Drug Discovery' at the Amsterdam Business School's…
Thanks to Prof. Onajomo "Ona" Akemu for giving me the opportunity to guest lecture on 'AI in Drug Discovery' at the Amsterdam Business School's…
Beliebt bei Maryam Khoshouei