Vivlion GmbH

👏 Congratulations to the PROXIDRUGS Zukunftscluster! We are happy to announce that Vivlion will be an associated partner of the #Cluster4Future in the now approved funding period! We are looking forward to work together with the consortium and accelerate drug discovery by contributing our bioinformatic know-how. So excited to test our latest analysis pipelines with real-world data!

#PRCISR CRISPR for solute carrier (#SLC) proteins: Check out our off-the-shelve pooled libraries targeting mouse or human SLC genes. Key features of the human version: 👉 425 target genes plus essential, non-targeting and safe-harbor controls 👉 1,868 gRNAs, gene list is available 👉 4 gRNAs per gene, 1 gRNA/plasmid, lentiviral backbone, puromycin resistance 👉 skew of 1.46   Key features of the mouse version: 👉 380 target genes plus essential, non-targeting and safe-harbor controls 👉 1,670 gRNAs, gene list is available 👉 4 gRNAs per gene, 1 gRNA/plasmid, lentiviral backbone, puromycin resistance 👉 skew of 1.59   All libraries are quality-controlled and come fully sequenced, adhering to Vivlion's commitment to superior uniformity and editing efficiency to allow you to downscale your experiments. Feel free to contact us for a quote!   Solute carrier proteins constitute a large and diverse group of membrane transport proteins that facilitate the movement of a wide array of substrates across cellular membranes, including ions, nutrients, neurotransmitters and metabolic intermediates. Given their fundamental role in cellular function, SLC proteins have been implicated in a multitude of diseases, including metabolic disorders, neurological conditions and cancer.   Despite their importance, many SLC proteins remain poorly understood due to the sheer number of SLC genes – over 400 in the human genome – and the complexity of their functional roles. The PRCISR™ CRISPR SLC libraries aim to functionally zoom into SLC genes, providing a powerful tool for researchers to elucidate the specific functions and regulatory mechanisms of these proteins and their roles in various diseases.   #SoluteCarriers #SLCProteins #CRISPRscreening #SLCs #NextGenerationCRISPRscreening #TransportProteins #PERTomics

We are excited to sponsor the 2nd CRISPR Medicine Conference, happening from April 7-11 in Copenhagen, Denmark. ➡ The website is now live and registration is open. Don’t miss this fantastic opportunity to connect with the #CRISPR community! We can't wait to return to Copenhagen for science, sports (join us for our #FunRun) and networking. In the meantime, contact us for all your #CRISPRscreening needs – we are happy to help! #CRISPRMED25 #FunctionalGenomics

Interesting interview by Fiona Behan PhD, GSK’s scientific director of target discovery, on combinatorial #CRISPR screens as powerful discovery tool. We couldn’t agree more to the fact that ➡ combinatorial CRISPR screening identifies complex dependencies/vulnerabilities in cancer ➡ sophisticated AI/ML approaches are needed to full reap the wealth of data generated by combinatorial screens ➡ thorough validation processes for identified targets are key to forwarding screening hits to the clinic At Vivlion, we aim to bring combinatorial CRISPR screening and analysis tools into every target discovery pipeline. Our multiplex CRISPR libraries can be tailored to any project needs and come in any dimension and format (all-by-all or fixed gene combinations). Regardless if you are a novice to combinatorial CRISPR screening or looking for a reliable partnership for your screening and analysis needs – do not hesitate to reach out and discuss your project with us! #PRCISR #CRISPR #mpxCRISPR #SyntheticGenes #targetdiscovery

To all #Ubiquitin, #UPS, #Proteasome and #TPD enthusiasts: We now offer a comprehensive human UPS #CRISPR library as part of our off-the-shelve portfolio at Vivlion. Key features: 👉 851 genes, essential, non-targeting and safe-harbor controls, 👉 total 3,416 gRNAs, gene list is available 👉 skew of 2.61  Don’t hesitate to reach out for a quote!   The utility of this type of library has only recently been proven in the discovery of a new modality in targeted protein degradation (#TPD), published in Nature (Hsia et al., 2024): An international research team led by Alessio Ciulli (CeTPD, University Dundee) and Georg Winter (CeMM, Vienna) , together with Manuel Kaulich, Martin Wegner, Dr. Dipl.-Bioinf., Koraljka Husnjak, and Ivan Đikić from Goethe University Frankfurt, defined the mode of action of a new class of bifunctional degraders termed intramolecular bivalent #glues (IBG) – a milestone in the field. They i.a. conducted a pooled #CRISPR screen utilizing #PRCISR technology to unravel IBG1’s mode of action.

Interesting publication of McCarthy et al. presenting a theory for high-throughput genetic interaction screens utilizing unique spectral barcodes for facilitating multiplexing. The elucidation of genetic interactions surely has been one of the holy grails of functional genomics. However, we do disagree with the notion that probing genetic interaction has been limited to about 100 pre-selected gene combinations in mammalian cells. Vivlion’s #multiplex dual gRNA technology has boosted the search space and now enables all-by-all combinations of two gene pools of arbitrary sizes. What can we provide? For example, using Vivlion’s #PRCISR platform, Diehl et al. (Nucleic Acids Res 2021) screened 12,736 autophagy gene combinations with 247,032 paired gRNAs for viability in reporter-based enrichment screens. They identified over 1,570 essential genetic interactions for #autophagy flux, including interactions among paralogous genes.   Reach out if you wish to learn more about our multiplex solutions in #CRISPRscreening!

Vivlion is deeply rooted in Germany's vibrant Rhine-Main area. As University spin-off, we sustain strong links to Goethe-Universität Frankfurt and Institute of Biochemistry II (IBC2) and are proud to be an associated partner to research networks such as PROXIDRUGS – ein BMBF-gefördertes Zukunftscluster. Together with Dr. Stefaniya G., we have been discussing how we plan the next growth step and why regional commitment to #biotech will be crucial in international location competition. #techtransfer #spinoff #startup

We had a great time at #CRISPRMED24 - a big thanks to the organizers at CRISPR Medicine News for a fantastic conference & bringing #CRISPR enthusiasts together for truly stimulating scientific discussions. A special thanks goes to all speakers of the #FunctionalGenomics session co-organized by #Vivlion: Jin Chen, Evgueni Ivakine, Fiona Behan PhD, Bernhard Schmierer, Daniel Schraivogel, Ziyi Sheng, Nikhil Gupta, PhD, Nutsa Burduli, Paul Diehl If you missed to visit us at our booth, don't hesitate to reach out to learn more about #PRCISR_CRISPR! Already looking forward to the 2nd CRISPR Medicine Conference, #CRISPRMED25 - stay tuned via CRISPR Medicine News. #design by artefont Tanja Labs

👏👏👏 to all runners! Thanks for joining our #FunRun at #CRISPRMED24. Great start into a fantastic conference day 💪

Don’t miss the #FunctionalGenomics session co-organized by Vivlion at #CRISPRMED24 today! 📢 Starting on Wednesday 24th April at 12:00 featuring Jin Chen from Altos Labs Evgueni Ivakine from The Hospital for Sick Children Fiona Behan PhD from GSK Manuel Kaulich from Goethe-Universität Frankfurt & #Vivlion 📢 Continuing after lunch break at 14:30 Bernhard Schmierer from Karolinska Institutet Daniel Schraivogel from EMBL Ziyi Sheng from University of Copenhagen (Københavns Universitet) Nikhil Gupta, PhD from Cancer Research UK (CRUK) Nutsa Burduli from Karolinska Institutet Paul Diehl from Cellecta, Inc. Happy to see many of you in the hall. ➡ Don't forget to come along at our booth C-1 to meet the Vivlion team Claudio Sabatelli, Elena Vialetto, Angela Hinchie, PhD and learn more about our #PRCISR_CRISPR technology! #CRISPRMED24 #CRISPRMedicineConference #CRISPR #FunctionGenomics #CRISPRlibraries #PRCISR_CRISPR