“Have worked with Frederik for more than a year at Novartis. He has a very good sense of detail and manages to keep track of a huge amount of different things at the same time. He knows a lot about what he is doing in this position. In spite of a huge workload he seems to never get truly stressed, and still gets the job done. He is also a nice guy with a good sense of humour.”
Frederik Knud Nielsen
Helsingør, Region Hovedstaden, Danmark
500+ forbindelser
Om
Strong interest in drug development, translational medicine and bringing people together that may have mutual interests across healthcare and the biopharmaceutical industry.
Pharmacist, PhD (toxicology) with broad experience from medical roles in the pharmaceutical industry - from translational medicine to medical affairs and building public private partnerships improving framework for health research.
Aktivitet
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We had a great SC meeting today for our DANLOGIC-HF trial. This RCT will hopefully revolutionize how we use telemonitoring and HL to guide care in…
We had a great SC meeting today for our DANLOGIC-HF trial. This RCT will hopefully revolutionize how we use telemonitoring and HL to guide care in…
Frederik Knud Nielsen synes godt om dette
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Som det første land i verden lancerer Danmark en side, der på sigt skal samle samtykker til forskningsprojekter. Kristoffer Sølvsten Burgdorf har…
Som det første land i verden lancerer Danmark en side, der på sigt skal samle samtykker til forskningsprojekter. Kristoffer Sølvsten Burgdorf har…
Frederik Knud Nielsen synes godt om dette
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How can we partner with our Health Care Systems to deliver better Patient Outcomes? This was at the center of our conversations during our Nordic…
How can we partner with our Health Care Systems to deliver better Patient Outcomes? This was at the center of our conversations during our Nordic…
Frederik Knud Nielsen synes godt om dette
Erfaring
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Novartis Danmark
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Steering group member
Data Saves Lives (Data Redder Liv)
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Novartis
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Steering Committee Member
NEXT partnership
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Pharmacist (Part time)
Dantrials
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Danish university of Pharmaceutical Sciences
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College visits organizer (part time while studying)
The Danish University of Pharmaceutical Sciences
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STORM counselor (part time while studying)
IVO*C Copenhagen
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Uddannelse
Udgivelser
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Incorporation of 14C-cholesterol in human adrenal corticocarcinoma H295R cell line and online-radiodetection of produced 14C-steroid hormone metabolites
Journal of Pharmaceutical and Biomedical Analysis
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Mixture Effects of 3 Mechanistically Different Steroidogenic Disruptors (Prochloraz, Genistein, and Ketoconazole) in the H295R Cell Assay
International Journal of Toxicology
Mixture effects of 3 model endocrine disruptors, prochloraz, ketoconazole, and genistein, on steroidogenesis were tested in the adrenocortical H295R cell line. Seven key steroid hormones (pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, estrone, and 17β-estradiol) were analyzed using gas chromatography and tandem mass spectrometry (GC-MS/MS) to investigate the effects throughout the steroidogenic pathway. Current modeling approaches often rely on models…
Mixture effects of 3 model endocrine disruptors, prochloraz, ketoconazole, and genistein, on steroidogenesis were tested in the adrenocortical H295R cell line. Seven key steroid hormones (pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, estrone, and 17β-estradiol) were analyzed using gas chromatography and tandem mass spectrometry (GC-MS/MS) to investigate the effects throughout the steroidogenic pathway. Current modeling approaches often rely on models assuming compounds acting independently and that the individual effects in some way can be summarized to predict a mixture effect. In H295R cells with an intact steroidogenic pathway, such assumptions may not be feasible. The purpose of this study was therefore to evaluate whether effects of a mixture with differing modes of action followed or deviated from additivity (concentration addition) and whether the H295R cell line was suitable for evaluating mixture toxicity of endocrine disruptors with different modes of action. The compounds were chosen because they interfere with steroidogenesis in different ways. They all individually decrease the concentrations of the main sex steroids downstream but exert different effects upstream in the steroidogenic pathway. Throughout the study, we observed lowest observed effect concentrations of mixtures at levels 2 to 10 times higher than the predicted EC50, strongly indicating antagonistic effects. The results demonstrate that chemical analysis combined with the H295R cell assay is a useful tool also for studying how mixtures of endocrine disruptors with differing modes of action interfere with the steroidogenic pathway and that existing models like concentration addition are insufficient in such cases. Furthermore, for end points where compounds exert opposite effects, no relevant models are available.
Andre forfattereSe udgivelse -
Corticosteroid Production in H295R Cells During Exposure to 3 Endocrine Disrupters Analyzed With LC-MS/MS
International Journal of Toxicology
The adrenocortical human cell line H295R is a valuable tool for screening endocrine disrupting compounds. In general, previous research focus has been on the production of the 2 sex steroids, 17β-estradiol and testosterone, and less attention has been paid to other important steroid end points in the steroidogenesis with a wide range of physiological functions, such as the glucocorticoids (corticosterone and cortisol). A newly developed and validated solid phase extraction (SPE) liquid…
The adrenocortical human cell line H295R is a valuable tool for screening endocrine disrupting compounds. In general, previous research focus has been on the production of the 2 sex steroids, 17β-estradiol and testosterone, and less attention has been paid to other important steroid end points in the steroidogenesis with a wide range of physiological functions, such as the glucocorticoids (corticosterone and cortisol). A newly developed and validated solid phase extraction (SPE) liquid chromatography–mass spectroscopy (LC-MS/MS) method was used to measure the production of cortisol and corticosterone in the H295R cell line. The method was applied by studying the effects of 2 model endocrine disrupters, ketoconazole and prochloraz, the pharmaceutical budesonide, and the inducer forskolin on the steroid production in this cell line. Dose–response curves were obtained for the correlation between hormone concentrations and the concentration of the individual disruptors. Exposing cells to ketoconazole resulted in a decrease in cortisol and corticosterone concentrations in a dose-dependent manner with EC50 values of 0.24 and 0.40 μmol/L, respectively. The same applied for cells exposed to prochloraz with EC50 values of 0.06 and 0.09 μmol/L for cortisol and corticosterone, respectively. Budesonide also inhibited glucocorticoid secretion. The EC50 value for cortisol was 19.50 μmol/L, whereas the EC50 value for corticosterone was 71.42 μmol/L. Forskolin induced the secretion of both cortisol (EC50 = 4.09 μmol/L) and corticosterone (EC50 = 0.28 μmol/L). The results obtained demonstrated the validity of the method. Based on these findings, quality criteria for the production of these steroids in this cell line were suggested.
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H295R cells as a model for steroidogenic disruption: A broader perspective using simultaneous chemical analysis of 7 key steroid hormones
Toxicology in vitro
The effects of three model endocrine disruptors, prochloraz, ketoconazole and genistein on steroidogenesis were tested in the adrenocortical H295R cell line to demonstrate that a broader mechanistic understanding can be achieved in one assay by applying chemical analysis to the H295R assay. Seven key steroid hormones (pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, estrone and 17β-estradiol) were analyzed using a novel and thoroughly validated GC-MS/MS…
The effects of three model endocrine disruptors, prochloraz, ketoconazole and genistein on steroidogenesis were tested in the adrenocortical H295R cell line to demonstrate that a broader mechanistic understanding can be achieved in one assay by applying chemical analysis to the H295R assay. Seven key steroid hormones (pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, estrone and 17β-estradiol) were analyzed using a novel and thoroughly validated GC-MS/MS method. In addition to the simultaneous quantification of 7 steroid hormones, the present method also negates the potential problems of cross-reactivity that can be encountered in some immunoassays. Although all 3 test compounds decrease the concentrations of the main sex steroids, the chemicals exerted different effects upstream in the pathway. Exposure to prochloraz resulted in increased hormone levels upstream of steroid 17 alpha-hydroxylase/17,20 lyase (P450c17) and decreases downstream. Ketoconazole inhibited the entire pathway, while exposure to genistein resulted in increased hormone levels upstream of 3-β-hydroxysteroid dehydrogenase (3β-HSD) and decreases downstream. The results demonstrate that chemical analysis combined with the H295R cell assay is a useful tool for studying the mechanisms by which endocrine disruptors interfere with the steroidogenic pathway.
Andre forfattereSe udgivelse -
Determination of steroid hormones in blood by GC–MS/MS
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
This paper presents the development, optimization and validation of a methodology to determine nine key steroid hormones (viz. pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, dihydrotestosterone, estrone, 17α-estradiol and 17β-estradiol) expressed in the steroidogenesis in biological fluids. The analytical method allows for the determination of steroid hormones in blood plasma and serum down to 0.08–0.16 ng/mL for estrogens, 0.20–0.36 ng/mL for androgens and…
This paper presents the development, optimization and validation of a methodology to determine nine key steroid hormones (viz. pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, dihydrotestosterone, estrone, 17α-estradiol and 17β-estradiol) expressed in the steroidogenesis in biological fluids. The analytical method allows for the determination of steroid hormones in blood plasma and serum down to 0.08–0.16 ng/mL for estrogens, 0.20–0.36 ng/mL for androgens and 0.36–0.43 ng/mL for progestagens. These limits of
detection were obtainable using a two-step solid-phase clean-up for fractionation and elimination of interfering lipids (fatty acids, phospholipids, glycerides and sterols) from the steroid hormones. The accuracy of the method was 50–112% in the range 0.10 to 2.00 ng/mLAndre forfattereSe udgivelse
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“Vi kan kun nå vores fælles mål gennem åben dialog og tæt samarbejde på tværs.” Sådan sagde sygehusdirektør Ricco Dyhr om partnerskaber, da han…
“Vi kan kun nå vores fælles mål gennem åben dialog og tæt samarbejde på tværs.” Sådan sagde sygehusdirektør Ricco Dyhr om partnerskaber, da han…
Frederik Knud Nielsen synes godt om dette
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"Close your eyes... Think about the last time where a person or a situation made you feel excluded, like you didn't belong... Think about the…
"Close your eyes... Think about the last time where a person or a situation made you feel excluded, like you didn't belong... Think about the…
Frederik Knud Nielsen synes godt om dette
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Maija Wolfin työ Novartiksella keskittyy tosielämän näyttöä tuottaviin tutkimuksiin. Mutta mitä RWE-tutkimus oikein on? Ja millaisia muutoksia syksy…
Maija Wolfin työ Novartiksella keskittyy tosielämän näyttöä tuottaviin tutkimuksiin. Mutta mitä RWE-tutkimus oikein on? Ja millaisia muutoksia syksy…
Frederik Knud Nielsen synes godt om dette
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Yesterday we had a very inspiring debate with Bogi Eliasen about the transition from "sick care to health care", and relevant "futures" we can…
Yesterday we had a very inspiring debate with Bogi Eliasen about the transition from "sick care to health care", and relevant "futures" we can…
Frederik Knud Nielsen synes godt om dette
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Fantastisk dag på Center for Klinisk Forskning, Regionshospital Nordjylland🚀🚀 De har knækket koden til det perfekte “Kursus i Klinisk Forskning og…
Fantastisk dag på Center for Klinisk Forskning, Regionshospital Nordjylland🚀🚀 De har knækket koden til det perfekte “Kursus i Klinisk Forskning og…
Frederik Knud Nielsen synes godt om dette
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We can’t wait to listen to Hudlægens bord (The Skin Doctor’s Table) – a new educational podcast on skin hosted by medical doctor and Ph.D. Iben Marie…
We can’t wait to listen to Hudlægens bord (The Skin Doctor’s Table) – a new educational podcast on skin hosted by medical doctor and Ph.D. Iben Marie…
Frederik Knud Nielsen synes godt om dette
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I am very pleased to announce that I have accepted a position as Senior Advisor/Negotiator at Amgros I/S from 1st of September. This also means that…
I am very pleased to announce that I have accepted a position as Senior Advisor/Negotiator at Amgros I/S from 1st of September. This also means that…
Frederik Knud Nielsen synes godt om dette
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Meget glad og en smule træt efter tre dage, hvor Novartis DK var værter for den årlige nordiske sommerkonference. Mange knoklede bag scenen, mens min…
Meget glad og en smule træt efter tre dage, hvor Novartis DK var værter for den årlige nordiske sommerkonference. Mange knoklede bag scenen, mens min…
Frederik Knud Nielsen synes godt om dette
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As Communications Advisors, should we “walk the talk” more? I have been fortunate to make a career out of doing what I love — advising bright people…
As Communications Advisors, should we “walk the talk” more? I have been fortunate to make a career out of doing what I love — advising bright people…
Frederik Knud Nielsen synes godt om dette