Galoz Kaneti
Formulations Lead
Haifa District, Israel
2K followers
500+ connections
Articles by Galoz
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Open Research Assistant position at a Nanotechnology and Immunotherapy Start-Up located in Haifa, Israel.
Open Research Assistant position at a Nanotechnology and Immunotherapy Start-Up located in Haifa, Israel.
By Galoz Kaneti
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Wanted: Biotechnology and Food Engineering MSc/PhDs from the Technion
Wanted: Biotechnology and Food Engineering MSc/PhDs from the Technion
By Galoz Kaneti
Activity
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We’ve been recognized by TheMarker as one of the Top 20 promising start-ups in Israel alongside PhaseV, Protai, caVos Biotherapeutics, and more…
We’ve been recognized by TheMarker as one of the Top 20 promising start-ups in Israel alongside PhaseV, Protai, caVos Biotherapeutics, and more…
Liked by Galoz Kaneti
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אני מגייסת Head of Clinical Affairs לחברת קומביניישן – מכשור רפואי משולב תרופה. הובלת ניסוי קליני פאזה שלישית החל מהתכנון ועד הגשות ל FDA, יחד עם…
אני מגייסת Head of Clinical Affairs לחברת קומביניישן – מכשור רפואי משולב תרופה. הובלת ניסוי קליני פאזה שלישית החל מהתכנון ועד הגשות ל FDA, יחד עם…
Liked by Galoz Kaneti
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The Technion is mentioning it's centennial- and our faculty finds that the most important accomplishment is our alumni! Seniors and juniors- we…
The Technion is mentioning it's centennial- and our faculty finds that the most important accomplishment is our alumni! Seniors and juniors- we…
Liked by Galoz Kaneti
Experience
Education
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Technion - Israel Institute of Technology
Doctor of Philosophy (Ph.D.) Biotechnology and food Engineering.
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In Prof. Amram Mor's lab, mainly focusing on novel antimicrobials development aimed to overcome antibiotics resistance in MDR S. aureus and to inhibit virulence factors induction related to the innate immune system.
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Technion - Israel Institute of Technology
Master of Science (M.Sc.) Biotechnology and Food Engineering.
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In Prof. Yechezkel Kashi's lab, mainly focusing on yeast genomics stationary phase mutations and molecular biology.
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Technion - Israel Institute of Technology
Bachelor of Science (B.Sc.) Biotechnology and Food Engineering
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Activities and Societies: Board member at the Technion Student Association- TSA. Chairman of the Biotechnology and Food Engineering Technion Student Association. Research project at the lab of Prof. Yechezkel Kashi, Faculty of Biotechnology and Food Engineering, Technion. Tutor of Organic Chemistry and protein biochemistry at The Beatrice Weston Center for the Advancement of Students, Technion. Tour guide at the Madatech- The Israel National Museum of Science. Student job at the Smoler Proteomics Center at the Technion.
Publications
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Pretreating Mesenchymal Stem Cells with Cancer Conditioned‐Media or Proinflammatory Cytokines Changes the Tumor and Immune Targeting by Nanoghosts Derived from these Cells
Advanced healthcare materials
Abstract
Nanoghosts (NGs) are nanovesicles reconstructed from the cytoplasmic membranes of mesenchymal stem cells (MSCs). By retaining MSC membranes, the NGs retain the ability of these cells to home in on multiple tumors, laying the foundations, thereby, for the development of a targeted drug delivery platform. The susceptibility of MSCs to functional changes, following their exposure to cytokines or cancer‐derived conditioned‐media (CM), presents the opportunity to modify the NGs by…Abstract
Nanoghosts (NGs) are nanovesicles reconstructed from the cytoplasmic membranes of mesenchymal stem cells (MSCs). By retaining MSC membranes, the NGs retain the ability of these cells to home in on multiple tumors, laying the foundations, thereby, for the development of a targeted drug delivery platform. The susceptibility of MSCs to functional changes, following their exposure to cytokines or cancer‐derived conditioned‐media (CM), presents the opportunity to modify the NGs by conditioning their source cells. This opportunity is investigated by comparing the membrane protein composition and the tumor uptake of NGs derived from naïve MSCs (N‐NG) against conditioned NGs made from MSCs pre‐treated with conditioned‐media (CM‐NG) or with a mix of the proinflammatory cytokines TNF‐α and IL‐1β (Cyto‐NG). CM‐NGs are found to be more targeted towards immune cells than Cyto‐ or N‐NGs, while Cyto‐NGs are the most tumor‐targeted ones, with similar immune‐targeting capacity as N‐NGs but with a higher affinity towards endothelial cells. Proteomic variations were wider in the CM‐NGs, with exceptionally higher levels of ICAM‐1 compared to N‐ and Cyto‐NGs. From a translational point of view, the data show that the tumor‐targeting ability of the NGs, and possibly that of other MSC‐derived extracellular vesicles, can be enhanced by simple conditioning of their source cells.Other authorsSee publication -
Radiolabeling of cell membrane-based nano-vesicles with 14C-linoleic acid for robust and sensitive quantification of their biodistribution
Journal of Controlled Release
Abstract
The rapid development of biomimetic cell membrane-based nanoparticles is still overshadowed by many practical challenges, one of which is the difficulty to precisely measure the biodistribution of such nanoparticles. Currently, this challenge is mostly addressed using fluorescent techniques with limited sensitivity, or radioactive labeling methods, which rarely account for the nanoparticles themselves, but their payloads instead. Here we report the development of a robust method for…Abstract
The rapid development of biomimetic cell membrane-based nanoparticles is still overshadowed by many practical challenges, one of which is the difficulty to precisely measure the biodistribution of such nanoparticles. Currently, this challenge is mostly addressed using fluorescent techniques with limited sensitivity, or radioactive labeling methods, which rarely account for the nanoparticles themselves, but their payloads instead. Here we report the development of a robust method for the innate radioactive labeling of cells and membrane-based nanoparticles and their consequent sensitive detection and biodistribution measurements. The preclinical potential of this method was demonstrated with Nano-Ghosts (NGs), manufactured from the cytoplasmic membranes of mesenchymal stem cells cultured with radioactively-labeled linoleic acid and achieving a cell labeling efficiency of 36%. Radiolabeling did not affect the physiochemical properties of the NGs, which stably retained their radiolabels. Using radioactivity measurements, we are now able to determine precisely the amount of NGs uptaken by tissues and cells, thereby providing further support to our presumed active NG targeting mechanisms. Biodistribution studies comparing radiolabeled NGs to fluorescently-labeled ones have validated our method and revealed new information, which could not be obtained otherwise, regarding the NGs' unique kinetics and rapid clearance, supporting their excellent safety profiles. The reported approach may be expanded to other membrane-based entities to facilitate and hasten their preclinical development and be used in parallel with other labeling methods to provide different and additional information.Other authorsSee publication -
Sensitization of Gram-negative Bacilli to Host Antibacterial Proteins
The Journal of Infectious Diseases
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Nanoghosts as a Novel Natural Nonviral Gene Delivery Platform Safely Targeting Multiple Cancers
Nano Letters
Nanoghosts derived from mesenchymal stem cells and retaining their unique surface-associated tumor-targeting capabilities were redesigned as a selective and safe universal nonviral gene-therapy platform. pDNA-loaded nanoghosts efficiently targeted and transfected diverse cancer cells, in vitro and in vivo, in subcutaneous and metastatic orthotopic tumor models, leading to no adverse effects. Nanoghosts loaded with pDNA encoding for a cancer-toxic gene inhibited the growth of metastatic…
Nanoghosts derived from mesenchymal stem cells and retaining their unique surface-associated tumor-targeting capabilities were redesigned as a selective and safe universal nonviral gene-therapy platform. pDNA-loaded nanoghosts efficiently targeted and transfected diverse cancer cells, in vitro and in vivo, in subcutaneous and metastatic orthotopic tumor models, leading to no adverse effects. Nanoghosts loaded with pDNA encoding for a cancer-toxic gene inhibited the growth of metastatic orthotopic lung cancer and subcutaneous prostate cancer models and dramatically prolonged the animals’ survival.
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Controlling bacterial infections by inhibiting proton-dependent processes
Biochimica et Biophysica Acta (BBA) - Biomembranes
Abstract
Bacterial resistance to antibiotics is recognized as one of the greatest threats in modern healthcare, taking a staggering toll worldwide. New approaches for controlling bacterial infections must be designed, eventually combining multiple strategies for complimentary therapies. This review explores an old/new paradigm for multi-targeted antibacterial therapy, focused at disturbing bacterial cytoplasmic membrane functions at sub minimal inhibitory concentrations, namely through…Abstract
Bacterial resistance to antibiotics is recognized as one of the greatest threats in modern healthcare, taking a staggering toll worldwide. New approaches for controlling bacterial infections must be designed, eventually combining multiple strategies for complimentary therapies. This review explores an old/new paradigm for multi-targeted antibacterial therapy, focused at disturbing bacterial cytoplasmic membrane functions at sub minimal inhibitory concentrations, namely through superficial physical alterations of the bilayer, thereby perturbing transmembrane signals transduction. Such a paradigm may have the advantage of fighting the infection while avoiding many of the known resistance mechanisms. This article is part of a Special Issue entitled: Antimicrobial peptides edited by Karl Lohner and Kai Hilpert.Other authorsSee publication -
Sensitization of Gram-negative bacteria to rifampin and OAK combinations
Scientific reports
Abstract
While individually inefficient against Gram-negative bacteria, in-vitro combinations of rifampin and OAK were mutually synergistic since sub-minimal inhibitory concentrations of one compound have potentiated the other by 2–4 orders of magnitude. Synergy persisted in-vivo as single-dose systemic treatment of Klebsiella infected mice resulted in 10–20% versus 60% survival, respectively accomplished by individual and combined compounds. This outcome was achieved without drug…Abstract
While individually inefficient against Gram-negative bacteria, in-vitro combinations of rifampin and OAK were mutually synergistic since sub-minimal inhibitory concentrations of one compound have potentiated the other by 2–4 orders of magnitude. Synergy persisted in-vivo as single-dose systemic treatment of Klebsiella infected mice resulted in 10–20% versus 60% survival, respectively accomplished by individual and combined compounds. This outcome was achieved without drug formulation, rather, pharmacokinetic considerations have inspired the therapeutic regimen.Other authors -
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Simultaneous breakdown of multiple antibiotic resistance mechanisms in S. aureus
Federation of American Societies for Experimental Biology
Abstract
In previous studies, the oligo-acyl-lysyl (OAK) C12(ω7)K-β12 added to cultures of gram-positive bacteria exerted a bacteriostatic activity that was associated with membrane depolarization, even at high concentrations. Here, we report that multidrug-resistant Staphylococcus aureus strains, unlike other gram-positive species, have reverted to the sensitive phenotype when exposed to subminimal inhibitory concentrations (sub-MICs) of the OAK, thereby increasing antibiotics potency…Abstract
In previous studies, the oligo-acyl-lysyl (OAK) C12(ω7)K-β12 added to cultures of gram-positive bacteria exerted a bacteriostatic activity that was associated with membrane depolarization, even at high concentrations. Here, we report that multidrug-resistant Staphylococcus aureus strains, unlike other gram-positive species, have reverted to the sensitive phenotype when exposed to subminimal inhibitory concentrations (sub-MICs) of the OAK, thereby increasing antibiotics potency by up to 3 orders of magnitude. Such chemosensitization was achieved using either cytoplasm or cell-wall targeting antibiotics. Moreover, eventual emergence of resistance to antibiotics was significantly delayed. Using the mouse peritonitis-sepsis model, we show that on single-dose administration of oxacillin and OAK combinations, death induced by a lethal staphylococcal infection was prevented in a synergistic manner, thereby supporting the likelihood for synergism to persist under in vivo conditions. Toward illuminating the molecular basis for these observations, we present data arguing that sub-MIC OAK interactions with the plasma membrane can inhibit proton-dependent signal transduction responsible for expression and export of resistance factors, as demonstrated for β-lactamase and PBP2a. Collectively, the data reveal a potentially useful approach for overcoming antibiotic resistance and for preventing resistance from emerging as readily as when bacteria are exposed to an antibiotic alone.Other authors -
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Ether–zymolyase ascospore isolation procedure: an efficient protocol for ascospores isolation in Saccharomyces cerevisiae yeast
Yeast
Abstract
Here we describe a new procedure for ascospore isolation from cultures containing a majority of unsporulated vegetative cells of Saccharomyces cerevisiae. The EZ ascospore isolation procedure relies on the combination of two conventional protocols, diethyl ether treatment and modified zymolyase treatment, allowing a significant increase in the efficiency of ascospore isolation and consequently enabling a large number of meiotic offspring to be efficiently obtained and screened, thus…Abstract
Here we describe a new procedure for ascospore isolation from cultures containing a majority of unsporulated vegetative cells of Saccharomyces cerevisiae. The EZ ascospore isolation procedure relies on the combination of two conventional protocols, diethyl ether treatment and modified zymolyase treatment, allowing a significant increase in the efficiency of ascospore isolation and consequently enabling a large number of meiotic offspring to be efficiently obtained and screened, thus improving the efficacy of genetic research and the genetic selection of S. cerevisiae strains. Copyright © 2010 John Wiley & Sons, Ltd.Other authorsSee publication
More activity by Galoz
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למורה שהבטיחה שלא יצא ממני כלום, את גרה בנס ציונה אז העיתון הזה הגיע אליך השבוע עד הדלת. גם אם לא פתחת אותו אני מקווה שלפחות נהנת מהשער. וזה לא כדי…
למורה שהבטיחה שלא יצא ממני כלום, את גרה בנס ציונה אז העיתון הזה הגיע אליך השבוע עד הדלת. גם אם לא פתחת אותו אני מקווה שלפחות נהנת מהשער. וזה לא כדי…
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I’m happy to share that I’ve started my PhD studies at the Technion-Israel Institute of Technology, Department of Chemistry, under the supervision of…
I’m happy to share that I’ve started my PhD studies at the Technion-Israel Institute of Technology, Department of Chemistry, under the supervision of…
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9 מתוך 20 החברות המבטיחות של הדה מרקר נוסדו על ידי בוגרי ובוגרות הטכניון! לרשימה המלאה הצטרפו לערוץ שלנו בוואטסאפ. לינק בתגובה לפוסט…
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Many security professionals assume that cross-site scripting (XSS) vulnerabilities are a thing of the past, impacting only a handful of rudimentary…
Many security professionals assume that cross-site scripting (XSS) vulnerabilities are a thing of the past, impacting only a handful of rudimentary…
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It was a pleasure to present our research in the Singapore International Food Forum. It was a great meeting with many interesting talks and friends.…
It was a pleasure to present our research in the Singapore International Food Forum. It was a great meeting with many interesting talks and friends.…
Liked by Galoz Kaneti
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Congratulations to Alice Joubran who has been highlighted in the Faculty of Biotechnology and Food Engineering, Technion's 'Project Star'! This…
Congratulations to Alice Joubran who has been highlighted in the Faculty of Biotechnology and Food Engineering, Technion's 'Project Star'! This…
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Join your local Technion Alumni group https://lnkd.in/dHjEE6ES Technion Alumni
Join your local Technion Alumni group https://lnkd.in/dHjEE6ES Technion Alumni
Liked by Galoz Kaneti
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I am happy and proud to share that I have successfully completed a year and a half-long Quality Engineering course (CQE)! 🎉 This journey was…
I am happy and proud to share that I have successfully completed a year and a half-long Quality Engineering course (CQE)! 🎉 This journey was…
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הכירו את פרויקט ZOË באמסטרדם, הולנד פרויקט עירוב שימושים שאפתני שמוקם בימים אלה במזרח העיר - ודוגל בשילוב של האדם והטבע יחד במבנה אחד. העיצוב הרב…
הכירו את פרויקט ZOË באמסטרדם, הולנד פרויקט עירוב שימושים שאפתני שמוקם בימים אלה במזרח העיר - ודוגל בשילוב של האדם והטבע יחד במבנה אחד. העיצוב הרב…
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