RNA-based gene therapy requires therapeutic RNA to function inside target cells without
eliciting unwanted immune responses. RNA can be ferried into cells using non-viral drug …

The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic
intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic …

The number and diversity of known CRISPR–Cas systems have substantially increased in
recent years. Here, we provide an updated evolutionary classification of CRISPR–Cas …

Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …

The December 2019 outbreak of a novel respiratory virus, SARS-CoV-2, has become an
ongoing global pandemic due in part to the challenge of identifying symptomatic …

The growing understanding of RNA functions and their crucial roles in diseases promotes
the application of various RNAs to selectively function on hitherto “undruggable” proteins …

CRISPR-Cas12a (Cpf1) proteins are RNA-guided enzymes that bind and cut DNA as
components of bacterial adaptive immune systems. Like CRISPR-Cas9, Cas12a has been …

The recent outbreak of novel coronavirus (SARS-CoV-2) causing COVID-19 disease
spreads rapidly in the world. Rapid and early detection of SARS-CoV-2 facilitates early …

Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen
detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered …

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases …