Improved collagen extraction by modified Longin method
TA Brown, DE Nelson, JS Vogel, JR Southon - Radiocarbon, 1988 - cambridge.org
TA Brown, DE Nelson, JS Vogel, JR Southon
Radiocarbon, 1988•cambridge.org… Based on our results, we suggest significant modifications to the Longin reflux conditions.
These … We suggest modification of the Longin collagen-extraction method on the basis of
a re-evaluation of the reflux conditions and examination of the products produced under
varied extraction conditions. The modifications involve refluxing in a weak acid solution (0.01
N HCl) at lower temperature (58C) followed by ultrafiltration to isolate the >30kD fraction of
the reflux product. Radiocarbon dating of the >30kD and <30kD fractions from several …
These … We suggest modification of the Longin collagen-extraction method on the basis of
a re-evaluation of the reflux conditions and examination of the products produced under
varied extraction conditions. The modifications involve refluxing in a weak acid solution (0.01
N HCl) at lower temperature (58C) followed by ultrafiltration to isolate the >30kD fraction of
the reflux product. Radiocarbon dating of the >30kD and <30kD fractions from several …
A re-evaluation of the Longin collagen-extraction method shows that a lower reflux temperature reduces degradation of protein (“collagen”) remnants. This allows additional purification through ultrafiltration to isolate the >30kDalton fraction of the reflux product.
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