Regulation of interferon-α responsiveness by the duration of Janus kinase activity
Daudi B lymphoblastoid cells are highly sensitive to the anti-growth and anti-viral effects of
interferon (IFN). Unlike many cell lines, these cells show prolonged transcription of IFN-
stimulated genes following treatment with IFN-α. This prolonged response correlated with
the continued presence of the activated transcription factor, IFN-stimulated gene factor 3
(ISGF3). Pulse-chase labeling experiments indicated that the half-life of the phosphorylation
of signal transducers and activators of transcription (Stat) 1 and Stat2 was short (< 2 h) …
interferon (IFN). Unlike many cell lines, these cells show prolonged transcription of IFN-
stimulated genes following treatment with IFN-α. This prolonged response correlated with
the continued presence of the activated transcription factor, IFN-stimulated gene factor 3
(ISGF3). Pulse-chase labeling experiments indicated that the half-life of the phosphorylation
of signal transducers and activators of transcription (Stat) 1 and Stat2 was short (< 2 h) …