CELLINK

Summer is nearing its end in the Northern Hemisphere, and with it an annual bump in skin cancers such as malignant melanoma. Creating in vitro tissue models of skin cancers that represent the tumor’s microenvironment will enable developments in melanoma therapy, as well as more accurate drug screening. Last week, the Centre of Excellence for Pharmaceutical Sciences at North West University Potchefstroom published an article titled “Pneumatic extrusion bioprinting-based high throughput fabrication of a melanoma 3D cell culture model for anti-cancer drug screening”. Maryke de Villiers et al set out to develop a simple, highly reproducible approach to developing a A375 melanoma 3D cell culture model. To do this, the research team used the BIO X 3D bioprinter as well as CELLINK’s GelMA A bioink. After further optimizing the printing parameters, the team printed cell-laden GelMA A droplets into a 96-well plate. Prior to drug-dosing, the team also created a 2D cell culture model. The cells showed a steadier proliferation rate in the 3D cell culture, and tended to aggregate into spheroids, whereas the 2D cell culture formed monolayer cell sheets. To evaluate the efficacy of the models, the team compared the drug responses in the two different cell culture types. The results indicate that the spheroids in the 3D cell culture model exhibited a higher level of drug resistance. This study highlights that 3D cell culture models – while a better environment for cells to thrive in – don’t add complexity to currently used assays. And that the 3D cell culture models have considerable potential for drug screening applications. Read the publication here: https://lnkd.in/dqkzDQ8j #3Dbioprinting #PublicationSpotlight #CancerResearch #Melanoma #DrugScreening

The BIONOVA X is our high-throughput DLP bioprinter, printing directly into well plates, enabling the creation of precise, complex biomimetic models. With bioprinting – you have the power to create what your research requires. For example, with multi-stiffness and multi-material capabilities, the BIONOVA X can create highly accurate diseased models that capture the smallest changes that would occur from the disease. This makes BIONOVA X an excellent option in some drug screening applications. Scroll through the slides below to learn more about the BIONOVA X and how it can help your research. #3dbioprinting #tissueengineering #BIONOVAX

Contracting the Zika Virus (ZIKV) comes with a risk of severe pregnancy complications, and has been linked to Guillain–Barré syndrome, an autoimmune disorder. In a paper from Shah et al. at Mercer University, titled “Buccal Administration of a Zika Virus Vaccine Utilizing 3D-Printed Oral Dissolving Films in a Mouse Model”, the researchers present a novel method for a pain-free, topical, delivery of a vaccine against ZIKV. Shah et al. used an INKREDIBLE+ 3D bioprinter to print oral dissolving films (ODFs). The ODFs consisted of various biodegradable biopolymers as well as ZIKV vaccine microparticles as previously developed by the group. Within mouse studies, the vaccine showed promising results that suggests potential protection against future Zika infections. While this proof-of-concept research shows great promise, the results also indicate that multiple vaccine doses may be necessary to achieve long-term immunity. In future research, the group will assess the vaccine’s neutralization ability in mice against a lethal dose of ZIKV. The researchers hope that the use of a pain-free vaccine alternative, like bioprinted ODFs, can contribute to enhanced regulatory compliance. We look forward to continuing to follow the group’s novel, innovative work. Read the publication: https://lnkd.in/daicCbiS #PublicationSpotlight #3DBioprinting #DrugDelivery #ZikaVirus #VaccineResearch

Researchers used Ruthenium photoinitiator to biofabricate and 3D bioprint decellularized ECM's. The ruthenium allows for visible light photocrosslinking of tryosine residues naturally present on in the extracellular matrix. Read the full publication here: https://lnkd.in/dGnjYAK5 #3dbioprinting #bioinks #research

We’re thrilled to announce that the registration is now open for the Bioprinting Ignited x Greece Conference and Workshop! In collaboration with Apel Laser, we look forward to welcoming you to Thessaloniki, Greece, from 15-16 October 2024 for an exceptional experience at KEDEA, Aristotle University of Thessaloniki (AUTH)'s research dissemination center. This event offers a unique opportunity to dive deep into the world of bioprinting technologies, explore cutting-edge trends, gain hands-on experience through interactive workshops, and connect with leaders in the bioprinting community. Register now to secure your spot, as workshop places are limited: https://lnkd.in/d_XrTScu For more information about the conference and to see the list of speakers, visit our website: https://lnkd.in/dTDbjhj8 #BioprintingIgnited #bioprintingconference #levelup #learn #networking #bioprintingleaders #technology #workshops #community #thessaloniki #greece

The BIO ONE empowers researchers to create reproducible 3D cell models that recapitulate in vivo environments. Whether developing new bioinks and hydrogels or looking to get better insights into the best drug candidates to carry along to clinical trials – we have designed the BIO ONE to facilitate an easy and seamless entrance to the world of 3D cell culture. Scroll through the slides below to learn more about the BIO ONE and its potential. #tissueengineering #cellculture #biodispensing #bioprinting #BIOONE

GelMA stands out as the go-to biomaterial for researchers in tissue engineering and 3D cell culture. Why? Because of its biocompatibility, tunable properties, and printability, GelMA is an extraordinarily versatile material. It mimics the extracellular matrix and promotes cell adhesion, proliferation, and differentiation. Together, these features of GelMA make it an excellent material for bioink formulations. GelMA is suitable for various bioprinting technologies, including extrusion-based and light-based bioprinters, allowing researchers to bioprint structures with great fidelity. We offer GelMA both as a lyophilizate (through Advanced BioMatrix’s PhotoGel®), as stock solutions and hydrogels, and as ready-to-use bioinks. To learn more about GelMA and its potential, read here: https://lnkd.in/dAbVWJSi #3DBioprinting #GelMA #Bioinks #CELLINK

With the Olympic Games in Paris about to kick into high gear, we want to highlight the exhibition: Match. Design & Sport – A Story Looking to the Future, displayed in Musée du Luxembourg in Paris. Match explores the role of design in sports progress and how the sporting world has inspired top designers. We were honored to be invited to display our and our partners’ work on personalized, regenerative medicine. From a patient’s scan to the biofabrication of a personalized model, our exhibit highlights how 3D bioprinting has the potential to radically change sports injury treatment in the future. If you find yourself in Paris, don't miss the chance to visit Musée du Luxembourg and discover the past and present innovations that pave the way for the breakthroughs of tomorrow. Watch our video on the TriAnkle project to learn more about how bioprinting can contribute to injury recovery, and get a sneak peek at the print we’re displaying! #3DBioprinting #TissueEngineering #PersonalizedMedicine #Paris2024 #Olympics2024

Today (July 22) is World Brain Day, dedicated to raising awareness about neurological disorders and brain health. Crucial to understanding neurological disorders, and finding treatments, is the ability to recreate human brain tissue in the lab. Researchers from Waisman Center at the University of Wisconsin-Madison published a paper titled "3D bioprinting of human neural tissues with functional connectivity" in @Cell Stem Cell. The research team used CELLINK's INKREDIBLE+ and BIO X to develop a 3D bioprinting platform for engineering reliable human neural tissue that is amenable to the dynamic functional assessment of neural circuits. The team developed a fibrin-hyaluronic acid hydrogel, which exhibited good printability and high cell viability in initial tests. The hydrogel is printed in sequential horizontal bands. This allowed for easy live-cell imaging as well as electrophysiological recording. The printed structures formed functional synaptic connections between neuronal subtypes within and across the printed bands, and functional neuron-astrocyte networks within 2-5 weeks after printing. Yuanwei Yan et al additionally assessed if the printed tissues were amenable to modeling pathological processes. Specifically, they looked at Alexander Disease (AxD), a rare and often fatal neurological disorder caused by a mutation in the GFAP gene. When printing with astrocytes derived from AxD, the researchers found that the AxD-loaded structures recapitulated the pathological feature of AxD. Conducting further tests and comparing them to an isogenic control (mutation corrected by CRISPR), the researchers found that the results indicate that the disease-relevant functional phenotypes are readily displayed in the printed human neural tissue. This bioprinting platform shows great promise for both studying cell interactions between specific neural cell types under pathological conditions, as well as a platform that's amenable to throughput analysis and drug development. Read the publication: https://lnkd.in/eT66WX9B #WorldBrainDay #PublicationSpotlight #3DBioprinting #TissueEngineering #NeuroScience #BrainTissueModels

Meet Elin Pernevik, a senior biomaterial scientist at CELLINK bioprinting, sharing her passion for innovation in #bioprinting technologies! 🧬 🖨️ Elin focuses on developing biomaterials for bio printing applications, pushing the boundaries of what’s possible in tissue engineering. At PRISM-LT, we’re thrilled to collaborate with experts like Elin, who bring top-notch knowledge across diverse research areas. Together, we’re pioneering the integration of bacteria, yeast, and stem cells to create hybrid living materials. 🦠 🧫 Discover more about our collaborative efforts at prism-livingtissues.eu and stay tuned for further updates from our dedicated team! 🔍 Special thanks to INsociety for facilitating these insightful conversations with our esteemed partners. 🙌 #Bioprinting #3Dbioprinting #TissueEngineering #Sustainability #CulturedMeat #Innovation #Biotech #HorizonEurope #EUeic #EUpathfinder #EuropeanProjects