Flavio Ortigao

The invention relates to oligoribonucleotide analogues with terminal 3'-3' or 5'-5' internucleotide linkages. This modification stabilises the molecules modified in this way, including ribozymes, without adversely changing their properties, including catalytic activities where appropriate.

The invention relates to oligoribonucleotide analogs with terminal 33 and/or 55 internucleotide linkages. This modification stabilizes the molecules altered in this way, including ribozymes, without adversely altering their properties, including, where appropriate, catalytic activities.

The present invention relates to arrays of discrete sensing elements of glycans (glycoarrays) wherein glycans are immobilized on a solid support (glycochips). Furthermore, the present invention relates to a method of producing such glycoarrays comprising immobilization of a glycan on a preferably streptaviding coated sensing surface via biotin or a derivative thereof. In addition, the present invention relates to the use of such glycoarrays for discriminating complex biological samples… Visa mer

The present invention relates to arrays of discrete sensing elements of glycans (glycoarrays) wherein glycans are immobilized on a solid support (glycochips). Furthermore, the present invention relates to a method of producing such glycoarrays comprising immobilization of a glycan on a preferably streptaviding coated sensing surface via biotin or a derivative thereof. In addition, the present invention relates to the use of such glycoarrays for discriminating complex biological samples, diagnosing a disease which correlates with the presence or absence of glycan binding molecules as well as to methods of identifying an organism by generation of signal pattern from a biological sample, which is indicative for the presence or absence of a particular organism. Furthermore, the present invention relates to a kit comprising the glycoarray of the invention useful in diagnostic assays. Visa mindre

Therapeutically utilizable oligonucleotides of the formulae I and II have been obtained by introduction of terminal 3'--3' and 5'--5' linkages. These compounds are stable to nucleases and suppress the biological function of nucleic acids.

The invention includes a method and apparatus for the sequence analysis of single- and double-stranded nucleic acids on solid phase supports whereby nucleic acids for the purpose of sequencing are terminally elongated with modified nucleic acid units, which themselves serve as units for anchoring to a water insoluble and solid polymer support. In the preferred mode, modified nucleotides which are to be attached to a nucleic acid chain by chemical or enzymatically catalyzed reactions are in… Visa mer

The invention includes a method and apparatus for the sequence analysis of single- and double-stranded nucleic acids on solid phase supports whereby nucleic acids for the purpose of sequencing are terminally elongated with modified nucleic acid units, which themselves serve as units for anchoring to a water insoluble and solid polymer support. In the preferred mode, modified nucleotides which are to be attached to a nucleic acid chain by chemical or enzymatically catalyzed reactions are in particular 5-bromo-2'-deoxyuridylate or in general other nucleotides halogenated or otherwise appropriately substituted at the base or sugar moieties, or streptavidin. Nucleic acids which can be immobilized in this way are either single-stranded DNA- or RNA-chains or such chains as parts of the double-stranded DNA or RNA. Water insoluble and solid supports are polymers such as cellulose, sepharose or sephadex as well as inorganic supports, in particular supports which have a backbone structure formed mainly by silicon and oxygen atoms. The water-insoluble solid supports have effector groups, in particular, they may contain immobilized antibodies specific to 5-bromo-2'-deoxyuridylate or other appropriate units capable of selective interaction with other halogenated nucleotides or nucleotides appropriately substituted at the base or sugar moieties. The binding of the nucleic acid strands which are to be sequenced to the polymer support is effected by interactions of the modified nucleotide units with the effector groups immobilized to the support, in particular by receptor ligand interactions. An apparatus is disclosed for carrying out the method. Visa mindre

The invention includes a method and apparatus for the sequence analysis of single- and double-stranded nucleic acids on solid phase supports whereby nucleic acids for the purpose of sequencing are terminally elongated with modified nucleic acid units, which themselves serve as units for anchoring to a water insoluble and solid polymer support. In the preferred mode, modified nucleotides which are to be attached to a nucleic acid chain by chemical or enzymatically catalyzed reactions are in… Visa mer

The invention includes a method and apparatus for the sequence analysis of single- and double-stranded nucleic acids on solid phase supports whereby nucleic acids for the purpose of sequencing are terminally elongated with modified nucleic acid units, which themselves serve as units for anchoring to a water insoluble and solid polymer support. In the preferred mode, modified nucleotides which are to be attached to a nucleic acid chain by chemical or enzymatically catalyzed reactions are in particular 5-bromo-2'-deoxyuridylate or in general other nucleotides halogenated or otherwise appropriately substituted at the base or sugar moieties, or streptavidin. Nucleic acids which can be immobilized in this way are either single-stranded DNA- or RNA-chains or such chains as parts of the double-stranded DNA or RNA. Water insoluble and solid supports are polymers such as cellulose, sepharose or sephadex as well as inorganic supports, in particular supports which have a backbone structure formed mainly by silicon and oxygen atoms. The water-insoluble solid supports have effector groups, in particular, they may contain immobilized antibodies specific to 5-bromo-2'-deoxyuridylate or other appropriate units capable of selective interaction with other halogenated nucleotides or nucleotides appropriately substituted at the base or sugar moieties. The binding of the nucleic acid strands which are to be sequenced to the polymer support is effected by interactions of the modified nucleotide units with the effector groups immobilized to the support, in particular by receptor ligand interactions. An apparatus is disclosed for carrying out the method. Visa mindre

A shipping container/rack for shipping and holding of specimen or culture tubes is claimed. The invention consists of a hollow housing with a plurality of wells for holding tubes upright during processing of their contents, and separate brackets for securing the tubes during shipment. The housings are joinable front-to-back for creating larger tube storage rack, and housings are also stackable one atop another. The housing provides a secure, shock-proof shipping container during shipping of… Visa mer

A shipping container/rack for shipping and holding of specimen or culture tubes is claimed. The invention consists of a hollow housing with a plurality of wells for holding tubes upright during processing of their contents, and separate brackets for securing the tubes during shipment. The housings are joinable front-to-back for creating larger tube storage rack, and housings are also stackable one atop another. The housing provides a secure, shock-proof shipping container during shipping of filled specimen tubes and a convenient tube rack for multiple tubes during insertion, extraction, and processing of tube contents. Visa mindre

Synthetic material (M1) biocompatible prod. (P) with a contact surface for biological material whose topology roughly corresponds to that of a material (M2) with a greater biocompatibility than the synthetic material.