Modern Implant Dentistry
()
About this ebook
Related to Modern Implant Dentistry
Related ebooks
Practical Periodontal Plastic Surgery Rating: 0 out of 5 stars0 ratingsTreatment Planning Single Maxillary Anterior Implants for Dentists Rating: 0 out of 5 stars0 ratingsMinimally Invasive Dental Implant Surgery Rating: 0 out of 5 stars0 ratingsCone Beam Computed Tomography: Oral and Maxillofacial Diagnosis and Applications Rating: 0 out of 5 stars0 ratingsDental Implant Complications: Etiology, Prevention, and Treatment Rating: 5 out of 5 stars5/5Supra-Gingival Minimally Invasive Dentistry: A Healthier Approach to Esthetic Restorations Rating: 5 out of 5 stars5/5Endodontology at a Glance Rating: 0 out of 5 stars0 ratingsMicrosurgery in Endodontics Rating: 0 out of 5 stars0 ratingsClinical Cases in Implant Dentistry Rating: 0 out of 5 stars0 ratingsImplantology in General Dental Practice Rating: 5 out of 5 stars5/5Quick Reference to Dental Implant Surgery Rating: 0 out of 5 stars0 ratingsEndodontic Radiology Rating: 0 out of 5 stars0 ratingsSuccessful Periodontal Therapy: A Non-Surgical Approach Rating: 0 out of 5 stars0 ratingsManagement of Complications in Oral and Maxillofacial Surgery Rating: 2 out of 5 stars2/5Platelet Rich Fibrin in Regenerative Dentistry: Biological Background and Clinical Indications Rating: 0 out of 5 stars0 ratingsDental Implant Treatment Planning for New Dentists Starting Implant Therapy Rating: 4 out of 5 stars4/5Contemporary Periodontal Surgery: An Illustrated Guide to the Art behind the Science Rating: 0 out of 5 stars0 ratingsProsthetic Treatment of the Edentulous Patient Rating: 0 out of 5 stars0 ratingsManaging Dental Trauma in Practice Rating: 5 out of 5 stars5/5Decision-Making for the Periodontal Team Rating: 0 out of 5 stars0 ratingsThe Osteoperiosteal Flap: A Simplified Approach to Alveolar Bone Reconstruction Rating: 4 out of 5 stars4/5Removable Prosthodontic Techniques Rating: 0 out of 5 stars0 ratingsToothwear: The ABC of the Worn Dentition Rating: 0 out of 5 stars0 ratingsKgmu Book of Clinical Cases in Dental Sciences Rating: 0 out of 5 stars0 ratingsAtlas of Operative Oral and Maxillofacial Surgery Rating: 5 out of 5 stars5/5Twenty-First Century Imaging Rating: 0 out of 5 stars0 ratingsTreatment Planning for the Developing Dentition Rating: 0 out of 5 stars0 ratingsDental Erosion Rating: 0 out of 5 stars0 ratingsManaging Endodontic Failure in Practice Rating: 4 out of 5 stars4/5Contemporary Restoration of Endodontically Treated Teeth: Evidence-Based Diagnosis and Treatment Planning Rating: 0 out of 5 stars0 ratings
Medical For You
The Lost Book of Simple Herbal Remedies: Discover over 100 herbal Medicine for all kinds of Ailment, Inspired By Dr. Barbara O'Neill Rating: 0 out of 5 stars0 ratingsWhat Happened to You?: Conversations on Trauma, Resilience, and Healing Rating: 4 out of 5 stars4/5The Vagina Bible: The Vulva and the Vagina: Separating the Myth from the Medicine Rating: 5 out of 5 stars5/5Mating in Captivity: Unlocking Erotic Intelligence Rating: 4 out of 5 stars4/5Gut: The Inside Story of Our Body's Most Underrated Organ (Revised Edition) Rating: 4 out of 5 stars4/5The Little Book of Hygge: Danish Secrets to Happy Living Rating: 4 out of 5 stars4/5Adult ADHD: How to Succeed as a Hunter in a Farmer's World Rating: 4 out of 5 stars4/5Brain on Fire: My Month of Madness Rating: 4 out of 5 stars4/5Mediterranean Diet Meal Prep Cookbook: Easy And Healthy Recipes You Can Meal Prep For The Week Rating: 5 out of 5 stars5/5The White Coat Investor: A Doctor's Guide to Personal Finance and Investing Rating: 4 out of 5 stars4/5The Grieving Brain: The Surprising Science of How We Learn from Love and Loss Rating: 4 out of 5 stars4/5Women With Attention Deficit Disorder: Embrace Your Differences and Transform Your Life Rating: 5 out of 5 stars5/5Living Daily With Adult ADD or ADHD: 365 Tips o the Day Rating: 5 out of 5 stars5/5This Is How Your Marriage Ends: A Hopeful Approach to Saving Relationships Rating: 4 out of 5 stars4/552 Prepper Projects: A Project a Week to Help You Prepare for the Unpredictable Rating: 5 out of 5 stars5/5The Diabetes Code: Prevent and Reverse Type 2 Diabetes Naturally Rating: 4 out of 5 stars4/5Herbal Healing for Women Rating: 4 out of 5 stars4/5The 40 Day Dopamine Fast Rating: 4 out of 5 stars4/5Peptide Protocols: Volume One Rating: 4 out of 5 stars4/5"Cause Unknown": The Epidemic of Sudden Deaths in 2021 & 2022 Rating: 5 out of 5 stars5/5Holistic Herbal: A Safe and Practical Guide to Making and Using Herbal Remedies Rating: 4 out of 5 stars4/5Blitzed: Drugs in the Third Reich Rating: 4 out of 5 stars4/5Extra Focus: The Quick Start Guide to Adult ADHD Rating: 5 out of 5 stars5/5The Emperor of All Maladies: A Biography of Cancer Rating: 5 out of 5 stars5/5ATOMIC HABITS:: How to Disagree With Your Brain so You Can Break Bad Habits and End Negative Thinking Rating: 5 out of 5 stars5/5
Reviews for Modern Implant Dentistry
0 ratings0 reviews
Book preview
Modern Implant Dentistry - Bart W. Silverman
1
Bone Metabolism Around Dental Implants
Richard J. Miron
Angel Insua
Summary
Despite the increasing number of studies in the field of implant dentistry investigating novel dental implant surfaces, biomaterials, and growth factors, comparatively very few have studied the biology and metabolism of bone healing and its implication in peri-implant tissue health. The aim of this chapter is to provide a thorough understanding of the biologic properties that impact bone formation and osseointegration, including the coupling mechanisms between immune cells and bone. This chapter focuses on the various bone cells in the body—osteocytes, bone lining cells (BLCs), osteoblasts, and osteoclasts—and their bone remodeling cycle. Furthermore, the importance of immune cells and their impact on biomaterial integration during bone formation and implant osseointegration is also discussed. Finally, the putative effects of cholesterol, hyperlipidemia, and vitamin D deficiency are addressed. Such factors should be monitored during patient care, and ultimately future research should focus on these avenues as well as meticulous maintenance programs to favor both early and long-term maintenance and stability of dental implants.
Objectives
▪Understand how overall patient health directly affects dental implant osseointegration
▪Understand the key cells involved in bone formation, maturation, and maintenance
▪Understand the direct role of immune cells on biomaterial and dental implant integration
▪Understand the essential role of optimizing the immune system prior to dental implant placement
▪Investigate the relationship between vitamin D deficiency and early implant failure and how to avoid such pitfalls
Bone regeneration requires bone grafting materials that possess excellent biocompatibility and osteoinductivity without eliciting an antigenic effect. While companies that manufacture replacement biomaterials intended to mimic autogenous bone grafts often report on their osteoconductive, osteoinductive, or osteogenic potential, autogenous bone still favors the greatest bone regeneration compared to allografts, xenografts, and synthetic alternatives because it combines all three of these properties. Thus, despite the increasing number of new bone grafting materials brought to market as substitute replacement grafts, to date there is no true replacement for autogenous bone grafts. 1 Autografts carry no risk of immunologic reaction or disease transmission and provide optimal conditions for the penetration of new blood vessels and migration of osteoprogenitor cells. In contrast, many bone grafting substitutes are osteoconductive but have limited osteoinductive potential. 2
For bone regeneration to take place, especially with foreign-body biomaterials such as allografts and xenografts or dental implants, there is a great need to better understand the regulatory properties and integration process of these biomaterials. After all, no matter the biomaterial placed, bone formation relies on immune-related factors working at the cellular level. The aim of this chapter is therefore to provide the biologic background on the cells involved in graft consolidation and give a brief overview of fracture healing. This chapter focuses on the bone cells involved in bone formation and dental implant osseointegration, including osteocytes, BLCs, osteoblasts, and osteoclasts, and their bone remodeling cycle. The chapter also addresses the importance of immune cells and their impact on biomaterial integration, as well as the putative effects of cholesterol, hyperlipidemia, and low vitamin D levels.
Bone Cells: Osteoclasts, Osteoblasts, and Osteocytes
There are three main cell types in bone tissue involved in the bone remodeling cycle: osteoclasts, osteoblasts, and osteocytes3 (Fig 1-1). Osteoclasts are the bone-resorbing cells that degrade bone tissues. They are derived from hematopoietic stem cells following their differentiation from monocytes in response to two key factors: receptor activator of nuclear factor kappa-B ligand (RANKL)4 and macrophage colony-stimulating factor (M-CSF).5 Osteoclasts can be characterized histologically based on their multinucleated morphology and expression of tartrate-resistant acid phosphatase (TRAP), cathepsin k (CatK), and the calcitonin receptor (CTR). Their formation, activity, and survival are also regulated by various hormones (such as calcitonin and estrogen) that regulate several downstream cytokines and cellular pathways.6 Activated osteoclasts form distinct and unique membrane domains, including the sealing zone, the ruffled border, and the functional secretory domain, which facilitate resorption of bone or bone graft particles.7 Rearrangement of their F-actin fibers from the cytoskeleton forms a ring shape consisting of a dense continuous zone of highly dynamic podosomes.8 These podosomes allow for mineralized bone to be gradually resorbed, creating grooves and tunnels on the bone surface. This process is also quite important for bone remodeling because the resorbed bone liberates calcium phosphates and growth factors contained within the bone matrix that attract bone-forming osteoblasts to the local environment.9
Fig 1-1
The bone remodeling cycle. Osteoclasts—cells that destroy and resorb bone—are present on the bone matrix surface and liberate growth factors and cytokines to the local microenvironment. These growth factors act on mesenchymal stem cells/progenitor cells to rapidly stimulate their proliferation and differentiation toward bone-forming osteoblasts. Osteoblasts lay new bone matrix and, once embedded within the bone matrix, become osteocytes.
Osteoblasts perform the opposite role of osteoclasts and are responsible for bone formation (see Fig 1-1). They are derived from cells of the mesenchymal lineage, and their formation and development are controlled locally and systemically by several growth factors, including bone morphogenetic proteins (BMPs).10,11 Osteoblasts secrete a range of molecules including growth factors, cell adhesion proteins, and other extracellular matrix molecules that support new bone formation.6 While osteoblasts are forming bone, they become embedded within bone tissues and transform into osteocytes.
Contrary to the short lifespan of osteoblasts and osteoclasts, osteocytes can live for decades within the bone matrix. They no longer produce new bone and instead undergo morphologic changes, losing cytoplasm organelles and acquiring a stellar shape morphology with numerous extensions that connect to other osteocytes through the canalicular network.12 Osteocytes can then transmit signals through this network similar to neuron communication; this communication has a profound impact on neighboring osteoblasts, osteoclasts, and osteocytes. While it was initially believed that osteocytes served only a mechanical transduction function,13 more recently their role has been deemed one of the most important within the bone tissues because they release numerous paracrine signals to their environment, thereby influencing both osteoblasts and osteoclasts.12,14,15
Fracture Healing and Graft Consolidation
Fracture healing is an important process that involves various cell types and a variety of signaling pathways.3,16,17 Unlike other tissues in the body, bone tends to regenerate and repair itself quite rapidly. Natural fracture healing is a four-stage process (Fig 1-2). The first step is hematoma formation. Following injury, a blood clot forms, creating a fibrin matrix with an abundance of infiltrating inflammatory and immune cells that take place with an activation of platelets.16,18 These cells secrete an array of growth factors that initiate the second and third phases of fracture repair.16,18 At the terminal end of the first phase, osteoclast formation occurs from precursor monocytes, and these osteoclasts invade the bone surface, commencing bone resorption.16
Fig 1-2
The fracture healing process is divided into four phases: (1) hematoma formation, (2) fibrocartilage callus formation, (3) bony callus formation, and (4) bone remodeling.
The second phase of fracture healing is the repair phase. During this phase, a bone callus is formed. Initially, blood vessels begin to form with infiltration of mesenchymal progenitor cells; this process is responsible for creating a fibrocartilagenous tissue that matures into bone. This woven bone is gradually replaced with dense lamellar bone to form a dense bony callus in phase 3.19
The final phase is the remodeling phase, during which the callus is gradually resorbed. In this stage, the bone is replaced by native bone lacking scar tissue.20 It has been shown that during this phase, resident macrophages play a predominant role in orchestrating host cells.21,22 While these four phases are not described in great detail within the present chapter, it is important to note that graft consolidation is tightly regulated by secretion of growth factors and cytokines (also secreted from autogenous bone particles and blocks). This is all tightly regulated in very distinct cell-to-cell communication events that take place during bone regeneration.3
Role of Osteocytes and Bone Lining Cells in Bone Remodeling
Osteocytes
Osteocytes are the pivotal cells in the regulation of bone mass and structure, along with osteoblasts and osteoclasts.23 Osteoblasts are derived from mesenchymal stem cells and synthesize new bone matrix.24 Osteoclasts are terminally differentiated multinucleated cells from the monocyte- macrophage lineage; beyond their resorbing bone function, these cells are also a source of cytokines that play an important role in bone homeostasis.25 Osteocytes are terminally differentiated osteoblasts, and their primary function is to support bone structure and mechanosensation.24 Osteocytes may act as regulators of bone remodeling by modulating osteoclast and osteoblast activities.25 These stellate-shaped cells are located within lacunae surrounded by mineralized bone matrix and present with connections through cytoplasmic prolongations with surface BLCs but also with bone marrow.15,25
Bone lining cells
BLCs are cells involved in bone formation much like preosteoblasts, osteoblasts, and osteocytes.26 They are characterized by a flat-shaped architecture along bony surfaces25 and may be considered latent osteoblasts.27 In human cancellous bone, around 65% of osteoblasts undergo apoptosis, with approximately 30% differentiating into osteocytes28; the reduced remnants become BLCs and chondroid-like cells.26,28 BLCs maintain their proliferative capability and often differentiate into other osteogenic cells.29,30 Various studies have shown that some factors can induce their proliferation prior to bone formation,31 while mature osteoblasts are unable to divide.26 Osteoblasts may also undergo a quiescent stage when there is no bone resorption or remodeling,29 but the function of BLCs might be more complex than a simple latent state,32 including catabolic and anabolic bone processes31 and rapid bone formation under osteogenic signaling.32
In the complex process of bone remodeling,33,34 external factors such as mechanical loading, irradiation, parathyroid hormone (PTH), fibroblast growth factor-2 (FGF2), sclerostin inhibition, or inflammation may lead BLCs to exit the quiescent stage and enter into an active function phase by re-forming their cuboidal appearance and their secretory capability.25,31,35 The presence of BLCs observed histologically indicates a strong sign of osteogenic potential29 and is often regarded as a major source of osteoblasts and proliferating preosteoblasts in the adult population.31 This prominent role in new bone formation was previously highlighted28,32 when rapid bone formation was observed after mechanical loading without previous bone resorption. Early peak bone formation after 3 days was only possible if BLCs underwent reactivation and reaquired their secretory capacities.28,32
Moreover, BLCs exert a prominent function during bone resorption,36 demonstrated by their ability to express key ostoclastogenesis markers including M-CSF receptor (M-CSFR), and after the modulation of bone resorption, BLCs play another important role in the early stages of bone formation by entering the resorption lacunae to remove collagen fibers and debris left by osteoclasts (Fig 1-3). Subsequent to this cleaning function, BLCs secrete a layer of fibrillar collagen, allowing osteoblasts to attach and deposit new osteoid.36
Fig 1-3
Bone remodeling after excessive implant torque. (1) Excessive torque promotes bone damage, including the osteocyte network. (2) Osteoblasts and osteoclasts are recruited from the blood, from the marrow, or from BLCs to populate the bone remodeling compartment. (3) Osteoclasts remove the damaged bone. (4) BLCs clean the debris after osteoclast resorption. (5) BLCs secrete fibrillar collagen. (6) This collagen layer allows osteoblasts to attach. (7) Osteoblasts deposit osteoid to fill the compartment. (8) Osteoblasts trapped in the osteoid become osteocytes or BLCs, after which most undergo apoptosis. (Reprinted with permission from Seeman.34)
Role of Macrophages in Bone Regeneration, Implant Osseointegration, and Breakdown
Macrophages play a prominent and central role in bone homeostasis and bone/biomaterial integration around dental implants.37 Specifically in bone tissues, a special subset of macrophages, termed osteal macrophages (or OsteoMacs), have recently been highlighted as playing a pivotal role in the fate of implant osseointegration.37 The general role of OsteoMacs in bone is to act as immune surveillance cells within their microenvironment.38,39 When a foreign-body biomaterial such as a dental implant is inserted transmucosally into alveolar bone, a rapid accumulation of macrophages is typically found at the implant surface.40 Chehroudi et al clearly showed that bone formation on titanium dental implant surfaces was routinely preceded by macrophage accumulation (prior to bone deposition).40 Despite this prominent finding, over 90% of research to date has focused on osteoblast and fibroblast behavior toward material surfaces, with only a small percentage (10%) dedicated to immune cell interactions including monocytes, macrophages, osteoclasts, leukocytes, and multinucleated giant cells (MNGCs).41 This major discrepancy is difficult to understand given the fact that macrophages and immune cells in general dictate how biomaterials will eventually be integrated into host tissues.
A series of key studies on OsteoMacs has shown that their removal during bone development is consistently associated with a reduction in bone modeling, bone remodeling, and bone repair.42–45 Furthermore, in primary osteoblast cultures (containing macrophages), the simple removal of macrophages from these in vitro systems leads to a 23-fold decrease in the mineralization potential of bone cells.44,46 Therefore, while basic studies have clearly pointed to their vast and substantial role in bone biology, much less information is available concerning the response of macrophages to implanted biomaterials such as bone grafts and dental implants. It is therefore crucial that we gain a better understanding of how immune cells and macrophages behave in relation to dental implant osseointegration and maintenance.
Macrophage polarization: M1 and M2 phenotypes
Macrophages are some of the most plastic cell types found in the human body. They polarize completely from the classical M1 macrophages (involved in tissue proinflammation) toward M2 (tissue regeneration) macrophages (Fig 1-4). They may also fuse into osteoclasts and resorb bone or fuse into MNGCs, where their role remains poorly defined.47,48 The primary difference between M1 and M2 macrophages is that M1 macrophages have their arginine metabolism shifted to nitric oxide (NO) and citrulline, whereas M2 macrophages are shifted toward ornithine and polyamines.49 M1 macrophages produce NO as a main effector molecule capable of inhibiting cell proliferation,50 while M2 macrophages generate ornithine, increasing cell proliferation and repair through polyamine and collagen synthesis.51
Fig 1-4
Macrophage polarization of both M1 and M2 phenotypes. M1 macrophages typically represent tissue inflammation and destruction. M2 macrophages are responsible for healing and tissue resolution.
During dental implant osseointegration, classical M1 macrophages secrete a wide array of proinflammatory cytokines including tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β), IL-6, IL-12, MMP-2, and MMP-9, typically induced by interferon gamma (IFN-γ) and lipopolysaccharide (LPS) or TNF-α (in vitro).50,52 In contrast, M2 macrophages are produced in response to IL-4 or IL-13 and also secrete a wide variety of proregenerative cytokines including platelet-derived growth factor BB (PDGF-BB), transforming growth factor beta 1 (TGF-β1), vascular endothelial growth factor (VEGF), IL-4, IL-10, and chemokine ligand 18 (CCL18). As can be expected, their polarization around implant surfaces is highly relevant for implant integration and long-term stability. Their role, especially as it relates to peri-implant infection, is extremely vital for the long-term maintenance of dental implants.
Macrophages, immune cells, and the foreign body reaction
It has been reported that implant osseointegration is a long-term equilibrium between host immune cells and bone biomaterials.53–55 The literature has showed that MNGC accumulation on implant surfaces leads to biomaterial breakdown and possible implant failure/rejection.53–55 These papers demonstrate that implant osseointegration and eventual peri-implant bone loss is likely a direct result of an M1-M2 shift in macrophage polarization. Interestingly, invading periodontal pathogens are known to secrete LPS, a known and direct molecule influencing proinflammatory M1 macrophage polarization.56 Hence, it is important to examine foreign body reaction, equilibrium between M1 and M2 macrophages, and MNGC polarization. Furthermore, these studies stressed heavily the material rejection with MNGC accumulation, further implicating the role of immune cells. As such, clinicians should always consider the dramatic importance and role of immune cells and general immune cell health during dental implant placement (see later section on vitamin D deficiency and chapter 2).
Bone Remodeling Around Dental Implants
After dental implants are anchored, a sequence of immune-inflammatory responses followed by angiogenesis and eventually osteogenesis take place to achieve osseo- integration. Initial protein adsorption is based on implant surface topography and hydrophilicity. Accordingly, thrombin and fibrinogen adhere to the implant surface. Later, neutrophils populate the implant recipient site before the monocytes and macrophages infiltrate the area. Cytokines and growth factors are then released and stimulate collagen matrix deposition around the titanium oxide layer, leading to newly formed woven bone (usually 5 days later). In a matter of 8 to 12 weeks, lamellar bone initiates biologic stability, or osseointegration.33
Just like the natural dentition, implants are subjected to soft and hard tissue remodeling after restoration delivery. The biologic width around dental implants has recently been shown to be about 3.5 mm, which is far greater than that around natural teeth.57 This physiologic bone remodeling mechanism to a foreign-body biomaterial is led by RANKL, which promotes macrophage activation into osteoclasts. It has been suggested that the microgap in two-piece implants might be associated with the presence of inflammatory cell infiltrate, which can lead to crestal bone loss by affecting both soft and hard tissues.58 A pumping effect of the liquid contained in the implant cavities may move into the peri-implant compartment due to the cyclical loading of the implant-abutment interface and facilitate the colonization of the gap and inner walls of the implant by gram-positive and gram-negative bacteria.58 These organic fluids with bacterial byproducts and endotoxins could upregulate the expression of proinflammatory cytokines in peri-implant tissues and stimulate the chemotaxis of active osteoclasts.58 Over time, leakage associated with micromovements can lead to a persistent inflammatory reaction and eventual bone loss around the implant neck as well as peri-implantitis.59 Research has hinted that internal implant connections provide a better seal than external ones, but either can provide a complete seal.60,61 Ongoing developments in implant and abutment design aim to limit this risk and reduce future crestal bone loss associated with microgap inflammation.
Effect of Excess Implant Torque on Bone Healing
Bone biology under implant insertion
Adequate implant insertion torque (IT) values (25–45 Ncm) have been suggested to prevent micromovements that could lead to fibrous encapsulation. On the flip side, high IT has also been associated with an increase in critical pressure, triggering microfractures and bone necrosis.62 It has been shown in animal models that high IT elicits a complex process of microdamage, which stimulated targeted bone remodeling.63 This was supported by a radiographic, histomorphometric, and histologic investigation that clearly demonstrated greater peri-implant bone loss in the early stages of healing for implants placed with a high IT (> 50 Ncm) compared with those more passively placed.64 High IT has been shown to lead to osteocyte apoptosis and consequently may promote higher levels of RANKL secretion to the surrounding environment to remove apoptotic cells.65,66 These findings highlight the importance of minimizing microfractures as a consequence of high IT to predictably preserve the peri-implant bone level. While a lack of primary stability may potentially jeopardize osseointegration, high IT might not favor the preservation of the peri-implant tissue level, so a reasonable level of torque must be achieved.
Alveolar bone density further influences primary stability. The maxillary ridge has been classified into four major types67; accordingly, denser bone is located in the anterior mandibular region, whereas more porous trabecular bone is detected in the posterior maxillary area. Recent findings seem to point to the influence of bone atrophy on bone density.68 Notwithstanding, the overlying cortex is mainly responsible for the mechanical stability of implants. Even when cortical bone has a higher elastic modulus69 and compressive strength than cancellous bone,70 the restrained vascularity of compact bone (ie, minimal to no migration of differentiating osteogenic cells) may result in peri-implant bone loss. Therefore, because of limited blood supply and lack of bone marrow, the number of osteoblasts in the bone remodeling area may be limited, which can prevent the area from reaching the critical osteoblastic cell density required for bone formation to occur.71,72
Crestal bone levels under high and low IT
For immediate implant placement with or without immediate loading, primary stability is necessary (> 32 Ncm).73,74 For delayed implant placement, on the other hand, an understanding of the resorption process of the bundle bone and the bone macroarchitecture and density might dictate the drilling sequence and IT applied. As such, implant placement performed with high IT (≥ 50 Ncm) has been shown to be prone to marginal bone loss and recession, notably in the presence of a thin buccal bone.75 Marginal bone loss was found to be substantially higher when higher IT thresholds (> 70 Ncm) were studied.76 This association was statistically significant when including all IT values (up to 176 Ncm).
Because of this risk of marginal bone loss, novel approaches for implant placement are being investigated. Simplified drilling methods are one proffered solution, and they do not seem to jeopardize the process of osseointegration.75,76 Wider implants installed under higher IT have shown adequate secondary stability and high bone-to- implant contact (BIC), although healing delay was reported due to necrosis of the existing bone.77 Findings from another group indicated that submerged implants inserted at 0 Ncm IT displayed similar outcomes at 4 months compared to those inserted at 30 or 70 Ncm.78 Clinical outcomes echo the uncertain impact of high IT on peri-implant bone loss compared to low IT. Future research is currently investigating alternative strategies including the application of osseodensification protocols,79 lasers,80,81 or ultrasound tools82–85 to enhance osseointegration.
Factors Affecting Bone Metabolism
Cholesterol and fatty acids
Within the last three decades, high-fat and high-cholesterol diets have become increasingly more prevalent in industrialized societies,86 and as a consequence, the morbidity and mortality of obesity-related diseases such as cardiovascular disease and hyper-inflamed conditions have also increased.87,88 Obesity is also associated with an enhanced risk of periodontal disease.89,90
Obesity and high levels of cholesterol production have been linked for years, but the relation between obesity and serum levels is low.91–93 Similarly, the relationship between bone and body fat is complex and not totally understood.94 Bone marrow fat (BMF) is the accumulation of fat cells inside the bone marrow tissue.95 An inverse correlation between bone mass and BMF has been reported.94–97 Higher adipogenesis in bone marrow may result in lower osteoblastogenesis, and these adipocytes can secrete saturated fatty acids that may impair osteoblast viability by inducing apoptosis and autophagy.95,96 Adipocytes can also release proinflammatory and osteoclastogenic cytokines (eg, TNF-α and IL-6) and adipokines and express RANKL.94,95,97–99
In other words, fatty acids96 and high levels of cholesterol100 may disturb the bone formation–bone resorption equilibrium by downregulating the Wnt signaling pathway.101 This is probably due to the effects of higher levels of TNF-α and sclerostin.102 The Wnt pathway balances the differentiation of mesenchymal stem cells by inhibiting adipogenesis and promoting osteoblast proliferation, maturation, and differentiation.96 Animal studies have shown more bone resorption, less bone formation and bone mass, and higher levels of bone turnover markers in subjects with high- cholesterol diets.96,100,103–105
In addition, obesity induces a systemic inflammation condition with high levels of circulating cytokines and increased production of monocytes, neutrophils,106,107 and adipose tissue macrophages.108,109 These cytokines and the accumulation of cholesterol in macrophages can alter the ratio of M1-M2 macrophages, promoting an M1 proinflammatory environment and thereby increasing the numbers of monocytes/macrophages in circulation.108,110
The influence of obesity and increased levels of cholesterol and triglycerides have been extensively described in the medical field, but the effect of hyperlipidemia on dental implant osseointegration has not yet been fully elucidated.111 Significantly more peri-implant bone loss, reduced bone formation, and lower strength in the bone-implant interface has previously been reported in mice after a 12-week high-fat diet.111 On the other hand, Dündar et al reported that there was no difference in BIC 12 weeks after implant placement between rabbits following a 3-month high-fat diet versus normal diet.112 Because hyperlipidemia might impair bone quantity and density, negative effects on implant osseointegration might be speculated, but no conclusive evidence to date has been found.
Vitamin D
The link between vitamin D deficiency and early implant failure has recently gained attention, with data demonstrating higher failure rates compared to even smoking and generalized periodontitis.113 Vitamin D is a fat-soluble hormone that regulates calcium phosphate homeostasis and mineral bone metabolism.114 It is transformed into the active form (1,25-dyhydroxyvitamin D3) by hydroxylation, first in the liver and then in the kidney.115 This vitamin can stimulate osteoblast bone matrix production, coupling bone resorption to formation and optimizing bone remodeling.116 It increases calcium absorption in the intestine, leading to a reduction in PTH secretion and lower systemic bone resorption115,117,118 with a possible inhibition of osteoclastogenesis.119 1,25-dyhydroxyvitamin D3 can stimulate bone resorption by binding to osteoblast vitamin D receptors (VDRs) and by altering the balance between RANKL and osteoprotogerin (OPG).120–123
Vitamin D is a common substance in the prevention and treatment of osteoporosis, but research investigating its direct effects on dental implant osseointegration has just begun.118 Kelly et al studied the effects of vitamin D deficiency on the osseointegration process in rats and reported up to 66% lower BIC values and mechanical bone strength 2 weeks after implant placement.124 Noteworthy is that the authors suggest that implant failure might be confounded by the rising deficiency of vitamin D prevalence in various patient populations.124 On the other hand, Zhou et al reported a significant increase in peri-implant bone density, BIC (1.5 times higher), and peri-implant trabecular microarchitecture following implant placement in rats who had undergone an 8-week regimen of oral vitamin D supplementation.118 Similar results were reported in mice with chronic kidney disease (CKD), suggesting that vitamin D treatment may be an effective approach for implant placement in patients with CKD.125 Recently, the effect of topical application of vitamin D (10%)126 and melatonin (5%)127 solutions on the surface of immediate implants placed in dogs was evaluated. Both topical applications significantly improved new bone formation around implants and reduced crestal bone loss at 12 weeks following surgery,127 demonstrating the positive correlation between vitamin D and early stages of osseointegration. In combination, these results suggest that vitamin D has a protective effect on bone healing after implant insertion. Chapter 2 further describes this link and the importance of vitamin D levels in implant patients.
Hyperglycemia
The number of adults with diabetes worldwide increased from 108 million in 1980 to 422 million in 2014.128 Type 1 diabetes (previously known as insulin-dependent, juvenile, or childhood-onset) is characterized by deficient insulin production and requires daily administration of insulin. Type 2 diabetes (formerly called non-insulin-dependent or adult-onset) results from the body’s ineffective use of insulin. Type 2 diabetes comprises the majority of people with diabetes around the world and is largely the result of excess body weight and physical inactivity.129 It is characterized by hyperglycemia, insulin resistance, and relative insulin deficiency.
Diabetes mellitus has been related to a deficient metabolism of the skeletal tissue due to a suppression in osteoblast function and lower bone formation potential, independent of the type of bone, the location, and mechanical loading.130 A higher risk of implant failure has been related to uncontrolled diabetes,131 and undiagnosed diabetes might be a possible reason for failed implants.132
Ajami et al reported delayed bone formation and remodeling in animals with hyperglycemia.132 Early bone mineralization might be affected due to a compromised intrafibrillar collagen mineralization, whereas interfibrillar and cement line mineralization remained normal.133 Diabetes also promotes a hypercoagulative state and a delay in fibrin clot resolution due to an increase in thrombin formation, platelet activation, and fibrin resistance.134 These events hinder platelet cytokines and growth factor release and cause limited pericyte and endothelial migration into the implant surface and thereby reduced angiogenesis.135
Other factors
Metabolic issues are not the only factors influencing bone remodeling. Medication, for example, might induce changes in bone cells and bone turnover and lead to bone loss around dental implants.136 Higher bone turnover seems to expose more implant surface, particularly in the mandible.136 Serotonin reuptake inhibitors have been related to an increase in bone loss and higher implant failure,137 so updated and thorough medical records are essential to avoid complications.
Hypersensitivity to titanium particles or ions released from the implant surface may also affect implant survival.136 Corrosion of the implant surface or degradation of the titanium dioxide layer can liberate wear particles that induce inflammatory reactions in the peri-implant tissues.138 In orthopedics, aseptic loosening is the main reason for long-term failures of hip and knee implants.139According to this model, wear particles are recognized as foreign-body substances and phagocytosed by macrophages.138 Later, M1 cells release inflammatory cytokines that promote osteoclastogenesis and osteolysis.138
Furthermore, it is important to note that certain antiseptic solutions commonly used in implant dentistry, such as chlorhexidine (CHX), have been shown to cause inflammation and/or fibroblast apoptosis, leading to M1 macrophage polarization. In cases following surgery, it is advised to avoid CHX because it may delay healing. Recent studies have shown nearly a 2,000-fold increase in release of inflammatory markers such as TNF-α when gingival fibroblasts were exposed to CHX.140
One novel replacement option that has shown promising results both before and after surgery is StellaLife’s recovery kit, a homeopathic wound healing rinse (Fig 1-5). It favors better wound healing of the defect site as well as improved pain management with microbial resistance.141 A split-mouth study revealed that the StellaLife VEGA Oral Care Recovery Kit performed better than many frequently utilized materials including Peridex, plasma rich in growth factors (PRGF), platelet-rich plasma (PRP), Emdogain, and PerioSciences products. The following conclusions were reported in the StellaLife test group141:
•The product achieved anesthetic pain relief.
•Healing was faster than with the control products.
•At 1 week, the sites treated with StellaLife resembled the other sites at 1 month (those treated with Peridex, PRGF, PerioSciences, PRP, or Emdogain).
•Less pain was associated with the sites treated with StellaLife.
•Fewer problems were reported for the sites treated with StellaLife.
•The pain level (rated as 5 or less the first day followed by less than 3 the consecutive days) was lower than anticipated (7 on a scale of 1 to 10).
Fig 1-5
StellaLife Recovery Kit used both before and after implant surgery.
StellaLife was initially developed to limit the need for narcotic drugs during healing and thereby battle the growing problem of narcotic dependence and addiction in the United States. In a study of 150 patients, all patients unanimously reported having fewer problems and recovering more quickly while utilizing StellaLife’s VEGA system compared to other products and therefore required fewer narcotic pain medications.
Conclusion
While implants have been highly researched over the years, it remains equally important to better understand how loading and implant bed preparation affect BLCs and osteocyte viability and signaling both at early and late time points. Furthermore, the effects of systemic levels of cholesterol, fatty acids, and vitamin D are important factors that may affect implant survival. In addition, the prominent role of immune cells (eg, OsteoMacs and MNGCs) on bone formation, bone remodeling, and implant osseointegration and maintenance must be researched further to discover how immune cells can be controlled to favor long-term stability and prevent peri-implant disease. Future research investigating these various topics is ongoing.
References
1. Jensen SS, Terheyden H. Bone augmentation procedures in localized defects in the alveolar ridge: Clinical results with different bone grafts and bone-substitute materials. Int J Oral Maxillofac Implants 2009;24(suppl):218–236.
2. Miron RJ, Zhang YF. Osteoinduction: A review of old concepts with new standards. J Dent Res 2012;91:736–744.
3. Gruber R, Stadlinger B, Terheyden H. Cell-to-cell communication in guided bone regeneration: Molecular and cellular mechanisms. Clin Oral Implants Res 2017;28:1139–1146.
4. Suda T, Takahashi N, Udagawa N, Jimi E, Gillespie MT, Martin TJ. Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocr Rev 1999;20:345–357.
5. Hofstetter W, Wetterwald A, Cecchini MC, Felix R, Fleisch H, Mueller C. Detection of transcripts for the receptor for macrophage colony- stimulating factor, c-fms, in murine osteoclasts. Proc Natl Acad Sci U S A 1992;89:9637–9641.
6. Civitelli R. Cell-cell communication in the osteoblast/osteocyte lineage. Arch Biochem Biophys 2008;473:188–192.
7. Mulari M, Vääräniemi J, Väänänen HK. Intracellular membrane trafficking in bone resorbing osteoclasts. Microsc Res Tech 2003;61: 496–503.
8. Luxenburg C, Geblinger D, Klein E, et al. The architecture of the adhesive apparatus of cultured osteoclasts: From podosome formation to sealing zone assembly. PloS One 2007;2:e179.
9. Nesbitt SA, Horton MA. Trafficking of matrix collagens through bone-resorbing osteoclasts. Science 1997;276:266–269.
10. Raisz LG, Kream BE. Regulation of bone formation. N Engl J Med 1983;309:29–35.
11. Cao X, Chen D. The BMP signaling and in vivo bone formation. Gene 2005;357:1–8.
12. Rochefort GY, Pallu S, Benhamou CL. Osteocyte: The unrecognized side of bone tissue. Osteoporos Int 2010;21:1457–1469.
13. Manolagas SC, Parfitt AM. What old means to bone. Trends Endocrinol Metab 2010;21:369–374.
14. Tatsumi S, Ishii K, Amizuka N, et al. Targeted ablation of osteocytes induces osteoporosis with defective mechanotransduction. Cell Metab 2007;5:464–475.
15. Bonewald LF. The amazing osteocyte. J Bone Miner Res 2011;26: 229–238.
16. Einhorn TA. The cell and molecular biology of fracture healing. Clin Orthop Relat Res 1998;(355 suppl):S7–S21.
17. Burchardt H. The biology of bone graft repair. Clin Orthop Relat Res 1983;(174):28–42.
18. Dimitriou R, Tsiridis E, Giannoudis PV. Current concepts of molecular aspects of bone healing. Injury 2005;36:1392–1404.
19. Bolander ME. Regulation of fracture repair by growth factors. Proc Soc Exp Biol Med 1992;200:165–170.
20. Marsell R, Einhorn TA. The biology of fracture healing. Injury 2011;42:551–555.
21. Alexander KA, Chang MK, Maylin ER, et al. Osteal macrophages promote in vivo intramembranous bone healing in a mouse tibial injury model. J Bone Miner Res 2011;26:1517–1532.
22. Shi C, Pamer EG. Monocyte recruitment during infection and inflammation. Nat Rev Immunol 2011;11:762–774.
23. Klein-Nulend J, Nijweide PJ, Burger EH. Osteocyte and bone structure. Curr Osteoporos Rep 2003;1:5–10.
24. Clarke B. Normal bone anatomy and physiology. Clin J Am Soc Nephrol 2008;3(suppl 3):S131–S139.
25. Florencio-Silva R, Sasso GR, Sasso-Cerri E, Simoes MJ, Cerri PS. Biology of bone tissue: Structure, function, and factors that influence bone cells. Biomed Res Int 2015;2015:421746.
26. Franz-Odendaal TA, Hall BK, Witten PE. Buried alive: How osteoblasts become osteocytes. Dev Dyn 2006;235:176–190.
27. Miller SC, Bowman BM, Smith JM, Jee WS. Characterization of endosteal bone-lining cells from fatty marrow bone sites in adult beagles. Anat Rec 1980;198:163–173.
28. Parfitt AM. Primary osteoporosis. Lancet 1980;1:773–774.
29. Miller SC, de Saint-Georges L, Bowman BM, Jee WS. Bone lining cells: Structure and function. Scanning Microsc 1989;3:953–960.
30. Bowman BM, Miller SC. The proliferation and differentiation of the bone-lining cell in estrogen-induced osteogenesis. Bone 1986; 7:351–357.
31. Matic I, Matthews BG, Wang X, et al. Quiescent bone lining cells are a major source of osteoblasts during adulthood. Stem Cells 2016;34:2930–2942.
32. Chow JW, Wilson AJ, Chambers TJ, Fox SW. Mechanical loading stimulates bone formation by reactivation of bone lining cells in 13-week-old rats. J Bone Miner Res 1998;13:1760–1767.
33. Davies JE. Understanding peri-implant endosseous healing. J Dent Educ 2003;67:932–949.
34. Seeman E. Bone modeling and remodeling. Crit Rev Eukaryot Gene Expr 2009;19:219–233.
35. Donahue HJ, McLeod KJ, Rubin CT, et al. Cell-to-cell communication in osteoblastic networks: Cell line-dependent hormonal regulation of gap junction function. J Bone Miner Res 1995;10:881–889.
36. Everts V, Delaisse JM, Korper W, et al. The bone lining cell: Its role in cleaning Howship’s lacunae and initiating bone formation. J Bone Miner Res 2002;17:77–90.
37. Miron RJ, Bosshardt DD. OsteoMacs: Key players around bone biomaterials. Biomaterials 2016;82:1–19.
38. Heinemann DE, Lohmann C, Siggelkow H, Alves F, Engel I, Koster G. Human osteoblast-like cells phagocytose metal particles and express the macrophage marker CD68 in vitro. J Bone Joint Surg Br 2000;82:283–289.
39. Ruiz C, Perez E, Vallecillo-Capilla M, Reyes-Botella C. Phagocytosis and allogeneic T cell stimulation by cultured human osteoblast-like cells. Cell Physiol Biochem 2003;13:309–314.
40. Chehroudi B, Ghrebi S, Murakami H, Waterfield JD, Owen G, Brunette DM. Bone formation on rough, but not polished, subcutaneously implanted Ti surfaces is preceded by macrophage accumulation. J Biomed Mater Res A 2010;93:724–737.
41. Thalji G, Cooper LF. Molecular assessment of osseointegration in vitro: A review of current literature. Int J Oral Maxillofac Implants 2014;29:e171–e199.
42. Davison NL, Gamblin AL, Layrolle P, Yuan H, de Bruijn JD, Barrere- de Groot F. Liposomal clodronate inhibition of osteoclastogenesis and osteoinduction by submicrostructured beta-tricalcium phosphate. Biomaterials 2014;35:5088–5097.
43. Hume DA, Loutit JF, Gordon S. The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80: Macrophages of bone and associated connective tissue. J Cell Sci 1984;66:189–194.
44. Chang MK, Raggatt LJ, Alexander KA, et al. Osteal tissue macrophages are intercalated throughout human and mouse bone lining tissues and regulate osteoblast function in vitro and in vivo. J Immunol 2008;181:1232–1244.
45. Favus MJ. Primer on the metabolic bone disease and disorders of mineral metabolism [in French]. Rev Fr Endocrinol Clin Nutr Metab 1996;37:553–554.
46. Pettit AR, Chang MK, Hume DA, Raggatt LJ. Osteal macrophages: A new twist on coupling during bone dynamics. Bone 2008;43:976–982.
47. Mosser DM, Edwards JP. Exploring the full spectrum of macrophage activation. Nat Rev Immunol 2008;8:958–969.
48. Martinez FO, Gordon S. The M1 and M2 paradigm of macrophage activation: Time for reassessment. F1000Prime Rep 2014;6:13.
49. Mills CD, Kincaid K, Alt JM, Heilman MJ, Hill AM. M-1/M-2 macrophages and the Th1/Th2 paradigm. J Immunol 2000;164:6166–6173.
50. MacMicking J, Xie QW, Nathan C. Nitric oxide and macrophage function. Ann Rev Immunol 1997;15:323–350.
51. Pesce JT, Ramalingam TR, Mentink-Kane MM, et al. Arginase-1- expressing macrophages suppress Th2 cytokine-driven inflammation and fibrosis. PLoS Pathog 2009;5:e1000371.
52. Novak ML, Koh TJ. Macrophage phenotypes during tissue repair. J Leukoc Biol 2013;93:875–881.
53. Albrektsson T, Dahlin C, Jemt T, Sennerby L, Turri A, Wennerberg A. Is marginal bone loss around oral implants the result of a provoked foreign body reaction? Clin Implant Dent Relat Res 2014;16:155–165.
54. Trindade R, Albrektsson T, Tengvall P, Wennerberg A. Foreign body reaction to biomaterials: On mechanisms for buildup and breakdown of osseointegration. Clin Implant Dent Relat Res 2016;18:192–203.
55. Trindade R, Albrektsson T, Wennerberg A. Current concepts for the biological basis of dental implants: Foreign body equilibrium and osseointegration dynamics. Oral Maxillofac Surg Clin North Am 2015;27:175–183.
56. Martinez FO, Sica A, Mantovani A, Locati M. Macrophage activation and polarization. Front Biosci 2007;13:453–461.
57. Tomasi C, Tessarolo F, Caola I, Wennström J, Nollo G, Berglundh T. Morphogenesis of peri-implant mucosa revisited: An experimental study in humans. Clin Oral Implants Res 2014;25:997–1003.
58. Ujiie Y, Todescan R, Davies JE. Peri-implant crestal bone loss: A putative mechanism. Int J Dent 2012;2012:742439.
59. Baixe S, Tenenbaum H, Etienne O. Microbial contamination of the implant-abutment connections: Review of the literature. Rev Stomatol Chir Maxillofac Chir Orale 2015;117:20–25.
60. Schmitt CM, Nogueira‐Filho G, Tenenbaum HC, et al. Performance of conical abutment (Morse Taper) connection implants: A systematic review. J Biomed Mater Res A 2014;102:552–574.
61. Berberi A, Maroun D, Kanj W, Amine EZ, Philippe A. Micromovement evaluation of original and compatible abutments at the implant- abutment interface. J Contemp Dental Pract 2016;17:907–913.
62. Monje A, Ravidà A, Wang HL, Helms JA, Brunski JB. Relationship between primary/mechanical and secondary/biological implant stability. Int J Oral Maxillofac Implants 2019;34:S7–S23.
63. Wang L, Ye T, Deng L, et al. Repair of microdamage in osteonal cortical bone adjacent to bone screw. PloS One 2014;9:e89343.
64. Duyck J, Corpas L, Vermeiren S, et al. Histological, histomorphometrical, and radiological evaluation of an experimental implant design with a high insertion torque. Clin Oral Implants Res 2010;21:877–884.
65. O’Brien CA, Nakashima T, Takayanagi H. Osteocyte control of osteoclastogenesis. Bone 2013;54:258–263.
66. Kennedy OD, Herman BC, Laudier DM, Majeska RJ, Sun HB, Schaffler MB. Activation of resorption in fatigue-loaded bone involves both apoptosis and active pro-osteoclastogenic signaling by distinct osteocyte populations. Bone 2012;50:1115–1122.
67. Misch CE. Bone classification, training keys to implant success. Dent Today 1989;8:39–44.
68. Monje A, Chan HL, Galindo-Moreno P, et al. Alveolar bone architecture: A systematic review and meta-analysis. J Periodontol 2015;86:1231–1248.
69. Rho JY, Tsui TY, Pharr GM. Elastic properties of human cortical and trabecular lamellar bone measured by nanoindentation. Biomaterials 1997;18:1325–1330.
70. Hayes WC. Biomechanics of cortical and trabecular bone: Implications for assessment of fracture risk. Basic Orthop Biomech 1991:93–142.
71. Kristensen HB, Andersen TL, Marcussen N, Rolighed L, Delaisse JM. Osteoblast recruitment routes in human cancellous bone remodeling. Am J Pathol 2014;184:778–789.
72. Andersen TL, Abdelgawad ME, Kristensen HB, et al. Understanding coupling between bone resorption and formation: Are reversal cells the missing link? Am J Pathol 2013;183:235–246.
73. Trisi P, Perfetti G, Baldoni E, Berardi D, Colagiovanni M, Scogna G. Implant micromotion is related to peak insertion torque and bone density. Clin Oral Implants Res 2009;20:467–471.
74. Ottoni JM, Oliveira ZF, Mansini R, Cabral AM. Correlation between placement torque and survival of single-tooth implants. Int J Oral Maxillofac Implants 2005;20:769–776.
75. Barone A, Alfonsi F, Derchi G, et al. The effect of insertion torque on the clinical outcome of single implants: A randomized clinical trial. Clin Implant Dent Relat Res 2016;18:588–600.
76. Khayat PG, Arnal HM, Tourbah BI, Sennerby L. Clinical outcome of dental implants placed with high insertion torques (up to 176 Ncm). Clin Implant Dent Relat Res 2013;15:227–233.
77. Jimbo R, Janal MN, Marin C, Giro G, Tovar N, Coelho PG. The effect of implant diameter on osseointegration utilizing simplified drilling protocols. Clin Oral Implants Res 2014;25:1295–1300.
78. Rea M, Lang NP, Ricci S, Mintrone F, Gonzalez Gonzalez G, Botticelli D. Healing of implants installed in over- or under-prepared sites—An experimental study in dogs. Clin Oral Implants Res 2015;26:442–446.
79. Lahens B, Neiva R, Tovar N, et al. Biomechanical and histologic basis of osseodensification drilling for endosteal implant placement in low density bone. An experimental study in sheep. J Mech Behav Biomed Mater 2016;63:56–65.
80. Sisti KE, de Andres MC, Johnston D, Almeida-Filho E, Guastaldi AC, Oreffo RO. Skeletal stem cell and bone implant interactions are enhanced by LASER titanium modification. Biochem Biophys Res Commun 2016;473:719–725.
81. Trisi P, Berardini M, Colagiovanni M, Berardi D, Perfetti G. Laser- treated titanium implants: An in vivo histomorphometric and biomechanical analysis. Implant Dent 2016;25:575–580.
82. Zhou HB, Hou YF, Chen WC, Shen JF, Wang J, Zhu ZM. [The acceleration of titanium implant osseointegration by low intensity pulsed ultrasound: An experimental study in rats]. Zhonghua Kou Qiang Yi Xue Za Zhi 2011;46:425–430.
83. Zhou H, Hou Y, Zhu Z, et al. Effects of low-intensity pulsed ultrasound on implant osseointegration in ovariectomized rats. J Ultrasound Med 2016;35:747–754.
84. Ustun Y, Erdogan O, Kurkcu M, Akova T, Damlar I. Effects of low- intensity pulsed ultrasound on dental implant osseointegration: A preliminary report. Eur J Dent 2008;2:254–262.
85. Sedlaczek J, Lohmann CH, Lotz EM, Hyzy SL, Boyan BD, Schwartz Z. Effects of low-frequency ultrasound treatment of titanium surface roughness on osteoblast phenotype and maturation. Clin Oral Implants Res 2017;28:e151–e158.
86. Tall AR, Yvan-Charvet L. Cholesterol, inflammation and innate immunity. Nat Rev Immunol 2015;15:104–116.
87. Devlin MJ, Rosen CJ. The bone-fat interface: Basic and clinical implications of marrow adiposity. Lancet Diabetes Endocrinol 2015;3:141–147.
88. Cavagni J, de Macedo IC, Gaio EJ, et al. Obesity and hyperlipidemia modulate alveolar bone loss in wistar rats. J Periodontol 2016;87: e9–e17.
89. Gorman A, Kaye EK, Apovian C, Fung TT, Nunn M, Garcia RI. Overweight and obesity predict time to periodontal disease progression in men. J Clin Periodontol 2012;39:107–114.
90. Nascimento GG, Leite FR, Do LG, et al. Is weight gain associated with the incidence of periodontitis? A systematic review and meta-analysis. J Clin Periodontol 2015;42:495–505.
91. Miettinen TA. Cholesterol production in obesity. Circulation 1971;44:842–850.
92. Hobbs MS, Knuiman MW, Briffa T, Ngo H, Jamrozik K. Plasma cholesterol levels continue to decline despite the rising prevalence of obesity: Population trends in Perth, Western Australia, 1980–1999. Eur J Cardiovasc Prev Rehabil 2008;15:319–324.
93. Bouillon K, Singh-Manoux A, Jokela M, et al. Decline in low-density lipoprotein cholesterol concentration: Lipid-lowering drugs, diet, or physical activity? Evidence from the Whitehall II study. Heart 2011;97:923–930.
94. Bermeo S, Gunaratnam K, Duque G. Fat and bone interactions. Curr Osteoporos Rep 2014;12:235–242.
95. Hardouin P, Pansini V, Cortet B. Bone marrow fat. Joint Bone Spine 2014;81:313–319.
96. During A, Penel G, Hardouin P. Understanding the local actions of lipids in bone physiology. Prog Lipid Res 2015;59:126–146.
97. Reid IR. Fat and bone. Arch Biochem Biophys 2010;503:20–27.
98. Melis D, Rossi A, Pivonello R, et al. Reduced bone mineral density in glycogen storage disease type III: Evidence for a possible connection between metabolic imbalance and bone homeostasis. Bone 2016;86:79–85.
99. Goto T, Nagai H, Egawa K, et al. Farnesyl pyrophosphate regulates adipocyte functions as an endogenous PPARgamma agonist. Biochem J 2011;438:111–119.
100. Soares EA, Nakagaki WR, Garcia JA, Camilli JA. Effect of hyperlipidemia on femoral biomechanics and morphology in low-density lipoprotein receptor gene knockout mice. J Bone Miner Metab 2012;30:419–425.
101. Baron R, Kneissel M. WNT signaling in bone homeostasis and disease: From human mutations to treatments. Nat Med 2013;19:179–192.
102. Baek K, Hwang HR, Park HJ, et al. TNF-alpha upregulates sclerostin expression in obese mice fed a high-fat diet. J Cell Physiol 2014;229:640–650.
103. Chen X, Wang C, Zhang K, et al. Reduced femoral bone mass in both diet-induced and genetic hyperlipidemia mice. Bone 2016;93:104–112.
104. Majima T, Shimatsu A, Komatsu Y, et al. Increased bone turnover in patients with hypercholesterolemia. Endocr J 2008;55:143–151.
105. Sanbe T, Tomofuji T, Ekuni D, Azuma T, Tamaki N, Yamamoto T. Oral administration of vitamin C prevents alveolar bone resorption induced by high dietary cholesterol in rats. J Periodontol 2007;78:2165–2170.
106. Swirski FK, Nahrendorf M. Leukocyte behavior in atherosclerosis, myocardial infarction, and heart failure. Science 2013;339:161–166.
107. Yvan-Charvet L, Pagler T, Gautier EL, et al. ATP-binding cassette transporters and HDL suppress hematopoietic stem cell proliferation. Science 2010;328:1689–1693.
108. Wei H, Tarling EJ, McMillen TS, Tang C, LeBoeuf RC. ABCG1 regulates mouse adipose tissue macrophage cholesterol levels and ratio of M1 to M2 cells in obesity and caloric restriction. J Lipid Res 2015;56:2337–2347.
109. Hill AA, Reid Bolus W, Hasty AH. A decade of progress in adipose tissue macrophage biology. Immunol Rev 2014;262:134–152.
110. Fadini GP, Simoni F, Cappellari R, et al. Pro-inflammatory monocyte- macrophage polarization imbalance in human hypercholesterolemia and atherosclerosis. Atherosclerosis 2014;237:805–808.
111. Keuroghlian A, Barroso AD, Kirikian G, et al. The effects of hyperlipidemia on implant osseointegration in the mouse femur. J Oral Implantol 2015;41:e7–e11.
112. Dündar S, Yaman F, Ozupek MF, et al. The effects of high-fat diet on implant osseointegration: An experimental study. J Korean Assoc Oral Maxillofac Surg 2016;42:187–192.
113. Guido Mangano F, Ghertasi Oskouei S, Paz A, Mangano N, Mangano C. Low serum vitamin D and early dental implant failure: Is there a connection? A retrospective clinical study on 1740 implants placed in 885 patients. J Dent Res Dent Clin Dent Prospects 2018;12:174–182.
114. Halfon M, Phan O, Teta D. Vitamin D: A review on its effects on muscle strength, the risk of fall, and frailty. Biomed Res Int 2015;2015:953241.
115. Holick MF. Vitamin D deficiency. N Engl J Med 2007;357:266–281.
116. Kogawa M, Findlay DM, Anderson PH, et al. Osteoclastic metabolism of 25(OH)-vitamin D3: A potential mechanism for optimization of bone resorption. Endocrinology 2010;151:4613–4625.
117. Choukroun J, Khoury G, Khoury F, et al. Two neglected biologic risk factors in bone grafting and implantology: High low-density lipoprotein cholesterol and low serum vitamin D. J Oral Implantol 2014;40:110–114.
118. Zhou C, Li Y, Wang X, Shui X, Hu J. 1,25Dihydroxy vitamin D(3) improves titanium implant osseointegration in osteoporotic rats. Oral Surg Oral Med Oral Pathol Oral Radiol 2012;114(5 suppl):S174–S178.
119. Sakai S, Takaishi H, Matsuzaki K, et al. 1-Alpha, 25-dihydroxy vitamin D3 inhibits osteoclastogenesis through IFN-beta-dependent NFATc1 suppression. J Bone Miner Metab 2009;27:643–652.
120. Leizaola-Cardesa IO, Aguilar-Salvatierra A, Gonzalez-Jaranay M, Moreu G, Sala-Romero MJ, Gomez-Moreno G. Bisphosphonates, vitamin D, parathyroid hormone, and osteonecrosis of the jaw. Could there be a missing link? Med Oral Patol Oral Cir Bucal 2016;21: e236–240.
121. Lieben L, Masuyama R, Torrekens S, et al. Normocalcemia is maintained in mice under conditions of calcium malabsorption by vitamin D-induced inhibition of bone mineralization. J Clin Investig 2012;122:1803–1815.
122. Horwood NJ, Elliott J, Martin TJ, Gillespie MT. Osteotropic agents regulate the expression of osteoclast differentiation factor and osteoprotegerin in osteoblastic stromal cells. Endocrinology 1998;139:4743–4746.
123. Schulze-Spate U, Dietrich T, Wu C, Wang K, Hasturk H, Dibart S. Systemic vitamin D supplementation and local bone formation after maxillary sinus augmentation—A randomized, double-blind, placebo-controlled clinical investigation. Clin Oral Implants Res 2016;27:701–706.
124. Kelly J, Lin A, Wang CJ, Park S, Nishimura I. Vitamin D and bone physiology: Demonstration of vitamin D deficiency in an implant osseointegration rat model. J Prosthodont 2009;18:473–478.
125. Liu W, Zhang S, Zhao D, et al. Vitamin D supplementation enhances the fixation of titanium implants in chronic kidney disease mice. PLoS One 2014;9:e95689.
126. Salomo-Coll O, Mate-Sanchez de Val JE, Ramirez-Fernandez MP, Hernandez-Alfaro F, Gargallo-Albiol J, Calvo-Guirado JL. Topical applications of vitamin D on implant surface for bone-to-implant contact enhance: A pilot study in dogs part II. Clin Oral Implants Res 2016;27:896–903.
127. Salomo-Coll O, de Mate-Sanchez JE, Ramirez-Fernandez MP, Hernandez-Alfaro F, Gargallo-Albiol J, Calvo-Guirado JL. Osseoinductive elements around immediate implants for better osteointegration: A pilot study in foxhound dogs. Clin Oral Implants Res 2018;29: 1061–1069.
128. Zhou B, Lu Y, Hajifathalian K, et al. Worldwide trends in diabetes since 1980: A pooled analysis of 751 population-based studies with 4.4 million participants. Lancet 2016;387:1513–1530.
129. World Health Organization. Definition, Diagnosis and Classification of Diabetes Mellitus and Its Complications: Report of a WHO Consultation. Part 1, Diagnosis and Classification of Diabetes Mellitus. 1999. https://apps.who.int/iris/handle/10665/66040 . Accessed 9 September 2022.
130. Retzepi M, Donos N. The effect of diabetes mellitus on osseous healing. Clin Oral Implants Res 2010;21:673–681.
131. Marchand F, Raskin A, Dionnes-Hornes A, et al. Dental implants and diabetes: Conditions for success. Diabetes Metab 2012;38:14–19.
132. Ajami E, Mahno E, Mendes V, Bell S, Moineddin R, Davies J. Bone healing and the effect of implant surface topography on osteoconduction in hyperglycemia. Acta Biomaterialia 2014;10:394–405.
133. Ajami E, Bell S, Liddell RS, Davies JE. Early bone anchorage to micro-and nano-topographically complex implant surfaces in hyperglycemia. Acta Biomaterialia 2016;39:169–179.
134. Carr ME. Diabetes mellitus: A hypercoagulable state. J Diabetes Complications 2001;15:44–54.
135. Oprea WE, Karp JM, Hosseini MM, Davies JE. Effect of platelet releasate on bone cell migration and recruitment in vitro. J Craniofac Surg 2003;14:292–300.
136. Bosshardt DD, Chappuis V, Buser D. Osseointegration of titanium, titanium alloy and zirconia dental implants: Current knowledge and open questions. Periodontol 2000 2017;73:22–40.
137. Wu X, Al-Abedalla K, Rastikerdar E, et al. Selective serotonin reuptake inhibitors and the risk of osseointegrated implant failure: A cohort study. J Dent Res 2014;93:1054–1061.
138. Pajarinen J, Kouri V-P, Jämsen E, Li T-F, Mandelin J, Konttinen YT. The response of macrophages to titanium particles is determined by macrophage polarization. Acta Biomaterialia 2013;9:9229–9240.
139. Sundfeldt M, Carlsson LV, Johansson CB, Thomsen P, Gretzer C. Aseptic loosening, not only a question of wear: A review of different theories. Acta Orthop 2006;77:177–197.
140. Fujioka-Kobayashi M, Schaller B, Pikos MA, Sculean A, Miron RJ. Cytotoxicity and gene expression changes of a novel homeopathic antiseptic oral rinse in comparison to chlorhexidine in gingival fibroblasts. Materials (Basel) 2020;13:3190.
141. Lee CY, Suzuki JB. The efficacy of preemptive analgesia using a non-opioid alternative therapy regimen on postoperative analgesia following block bone graft surgery of the mandible: A prospective pilot study in pain management in response to the opioid epidemic. Clin J Pharmacol Pharmacother 2019;1:26–31.
2
Vitamin D Deficiency and Early Implant Failure
Richard J. Miron
Summary
Dental implants are generally considered a very safe and highly predictable surgical procedure, yet each year a number of implants placed into adequate bone volume are lost within the first 8 weeks of healing. This chapter describes how nutritional deficiencies, namely that of vitamin D, are partly to blame. Vitamin D deficiency is one of the most prominent and known deficiencies in modern industrialized societies. Vitamin D is a fat-soluble vitamin critical for proper immune function as well as bone homeostasis. Recent dental implant studies have demonstrated that while smoking and generalized periodontitis are associated with a 50% to 200% increase in dental implant failure, vitamin D deficiency is associated with up to a 300% increase in early implant failure. These shocking findings further highlight the fact that systemic health, including adequate vitamin and mineral intake, play a critical role in biomaterial/dental implant integration.
This chapter briefly presents recent research on the prominent links between vitamin deficiencies (particularly vitamin D) and early implant failure. Thereafter, a quick and easy in-office testing kit for vitamin D is presented that uses a simple finger prick, similar to glucose testing. Finally, supplementation guidelines and recommendations from the American Association of Clinical Endocrinologists (AACE) and the American College of Endocrinology (ACE) are presented for deficient patients with the aim of minimizing early implant failure potentially caused by vitamin/mineral deficiencies.
Objectives
▪Understand the important epidemiologic studies linking higher immune-related disorders among the US population and dental implant failure rates
▪Learn how a lack of general health and increased use of medications may alter proper immune cell health
▪Discover how vitamin D deficiency in the US population affects bone metabolism
▪Understand the links between vitamin D deficiency and early implant failure
▪Learn how to test vitamin D levels in the office in 15 minutes
▪Understand proper supplementation guidelines for before and after implant placement
Vitamin D deficiency is a worldwide public health problem that spans all age groups from children to adults. As we age, our ability to absorb vitamin D naturally decreases, and very few foods naturally contain sufficient doses. Of course we have a wonderful source of vitamin D available to us—the sun—but with most of the population working indoors, the majority of people in industrialized nations do not receive enough sunlight daily to maintain sufficient levels of vitamin D. As a result, epidemiologic studies report that roughly 70% of society worldwide is deficient. 1
Vitamin D deficiency is most known for its association with osteoporotic and postmenopausal women. Few realize, however, its drastic and substantial role in various other diseases. These include depression, dementia, Alzheimer’s disease, asthma, cancer, cardiovascular disease, and diabetes, among others highlighted throughout this book. Vitamin D is essential for gastrointestinal calcium absorption, mineralization of osteoid tissue, and maintenance of serum ionized calcium level. It is also important for other physiologic functions, such as muscle strength, neuromuscular coordination, and hormone release.2 More recently, vitamin D deficiency has also been associated with up to a 300% increase in dental implant failure, and more associations with other dental-related complications are being discovered as well.3–12 Optimizing levels prior to surgery therefore becomes fundamental for maximizing wound healing. This chapter discusses the association between vitamin D deficiency and dental implant–related failures and bone grafting complications.
Understanding Foreign Body Reactions and Health
Importance of the immune system
Many years ago, scientists believed that it was bone cells (osteoblasts and osteoclasts) that would interact with a dental implant and, following an integration period, lay down new bone matrix for a happy coexistence of the implant within the body. However, modern research has shown that it is not bone cells that interact with this newly introduced biomaterial but in fact immune cells that gather around it. It is the immune system that dictates whether the biomaterial will be accepted and integrate within the body or be rejected altogether. It is the immune system that is ultimately responsible for the integration of any foreign substance.13 Therefore, when an individual has problems relating to the immune system, dental implant complications (ie, failure to integrate) may occur. That is why it is so vital that a healthy immune system is maintained.
Poor health in the United States
Despite boasting some of the best medical institutions, hospitals, and universities in the world, the United States has one of the sickest populations in the world. Americans take the most medication per capita, and their average life expectancy is much lower than the populations of comparable industrialized nations (Fig 2-1). Over the past 70 years, life expectancy has consistently risen globally in industrialized countries, as health care and our understanding of science and medicine has improved. However, in 2014, the life expectancy for the United States population leveled and even dipped slightly, and it has not recovered or increased since, while all other comparable countries continued their upward trend. Evidence shows that this decline in US life expectancy is directly linked to the declining nutrition we eat and the unhealthy lifestyle we live here in the United States, both of which impact our immune systems and their ability to fight disease. Even US children have seen an alarming rise in immune-related disorders; child allergies have increased over tenfold in the last 50 years.14,15
Fig 2-1
Life expectancy in the United States versus other industrialized countries. Since 2014, life expectancy has leveled in the United States and even seen a slight dip, whereas it has continued to trend upward in almost all other comparable countries.
This reality of poor immune health in the United States is important to consider because practicing dentists are placing biomaterials into more and more patients with compromised immune systems, which means greater risk of early implant failure and biomaterial-related complications.
Vitamin D deficiency is one of the most concerning issues in terms of immune health. Vitamin D is a powerful immunomodulator, and without it the immune system does not function as efficiently, thus making patients more susceptible to immune-related problems including allergies and compromised biomaterial integration. With adults and children spending more time than ever before indoors, the rate of vitamin D deficiency among the global population has almost doubled in the past decade alone.16 This means that clinicians are placing implants into less healthy patients, and, despite recent improvements in biomaterial compatibility, osseointegration outcomes will be affected, thereby affecting implant failure rates as well.
Understanding Vitamin D and Its Optimal Levels
A reliable marker of vitamin D status is serum 25-hydroxy vitamin D (25-OHD), and a level below 20 ng/mL defines deficiency. Levels above 30 ng/mL are required to maximize the bone health and nonskeletal benefits of vitamin D (Table 2-1). For individuals undergoing any type of dental-related procedures, levels between 40 and 60 ng/mL are generally recommended, because levels may decrease significantly following a period of physical stress (eg, a dental surgical intervention).
Table 2-1 Vitamin D concentrations in humans from deficient to toxic
Unfortunately, foods do not contain sufficient concentrations of vitamin D to maintain appropriate levels for immune health. Even the foods with the most vitamin D—cod liver oil (400–1,000 IU/teaspoon), fresh caught salmon (600–1,000 IU/ 3.5 oz vitamin D3), tuna (236 IU/3.5 oz vitamin D3), egg yolk (20 IU/yolk vitamin D3 or D2), and fortified milk, cheese, or yogurt (100 IU/3 oz, usually vitamin D3)—contain relatively low concentrations of vitamin D, considering deficiency should be treated with 5,000 to 10,000 IU/day for a 4- to 12-week period to restore vitamin D sufficiency.
According to the American Association of Clinical Endocrinologists (AACE) and the American College of Endocrinology (ACE) guidelines, it is recommended that supplementation maintain levels above 30 ng/mL.17 The Endocrine Society in the USA recommends achieving a concentration of more than 30 ng/mL (> 75 nmol/L) of 25-OHD. The Endocrine Society also advocates an intake of 1,500 to 2,000 IU/day (37.5–50 μg) in all adults, and obese patients (BMI > 30) should take as much as three times that.17
Dental-Related Complications Associated with Vitamin D Deficiency
In addition to supporting the immune system and biomaterial integration, vitamin D decreases general oxidative stress and minimizes additional inflammation caused by surgery. Therefore, vitamin D deficiency can lead to various complications in the dental field.
In 2009, a study investigated the role of vitamin D on dental implant osseointegration in rats.9 In this study, implants were placed in