Colin Ng

Colin Ng

New Haven, Connecticut, United States
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About

Adaptable, energetic, results driven project leader with significant experience in…

Activity

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Experience

  • AtlasXomics Inc. Graphic

    AtlasXomics Inc.

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    New Haven, Connecticut, United States

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    Branford, CT

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    Branford, CT

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    Branford, CT

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    Branfod, CT

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    Boston, MA

Education

Licenses & Certifications

Publications

  • Enhanced TLR2 Responses in Multiple Sclerosis.

    Clinical and Experimental Immunology

  • Preinfusion Polyfunctional Anti-CD19 Chimeric Antigen Receptor T Cells Associate with Clinical Outcomes in NHL

    Blood

  • Aligned fibers direct collective cell migration to engineer closing and nonclosing wound gaps.

    Molecular biology of Cell

  • CD8+ T-Cell Mediated Anti-Malaria Protection Induced by Malaria Vaccines; Assessment of Hepatic CD8+ T Cells by SCBC Assay

    Human Vaccines & Immunotherapeutics

  • Single-Cell Multiplexed Cytokine Profiling of CD19 CAR-T Cells Reveals a Diverse Landscape of Polyfunctional Antigen-Specific Response

    Journal for ImmunoTherapy of Cancer

  • Shape-Dependent Cell Migration and Focal Adhesion Organization on Suspended and Aligned Nanofiber Scaffolds

    Acta Biomaterialia

    In the body, cells dynamically respond to chemical and mechanical cues from the extracellular matrix (ECM), yet precise mechanisms by which biophysical parameters (stiffness, topography and alignment) affect cell behavior remain unclear. Here, highly aligned and suspended multilayer polystyrene (PS) nanofiber scaffolds are used to study biophysical influences on focal adhesion complex (FAC) arrangement and associated migration behavior of mouse C2C12 cells arranged in specific shapes: spindle…

    In the body, cells dynamically respond to chemical and mechanical cues from the extracellular matrix (ECM), yet precise mechanisms by which biophysical parameters (stiffness, topography and alignment) affect cell behavior remain unclear. Here, highly aligned and suspended multilayer polystyrene (PS) nanofiber scaffolds are used to study biophysical influences on focal adhesion complex (FAC) arrangement and associated migration behavior of mouse C2C12 cells arranged in specific shapes: spindle, parallel and polygonal. Furthermore, the role of cytoskeletal-altering drugs including blebbistatin, nocodazole and cytochalasin-D on FAC formation and migratory behavior is investigated. For the first time, this work reports that cells on suspended fiber networks, including cells with administered drugs, elongated along the fiber axes and developed longer (∼ 4×) and more concentrated FAC clusters compared to cells on flat PS control substrates. Additionally, substrate designs which topographically restrict sites of cell attachment and align adhesions were found to promote higher migration speeds (spindle: 52 μm h−1, parallel: 39 μm h−1, polygonal: 25 μm h−1, flat: 32 μm h−1). This work demonstrates that suspended fiber topography-induced concentration of FACs along fiber axes generates increased migration potential as opposed to flat surfaces, which diffuse and randomly orient adhesions.

    Other authors
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Patents

  • Systems, devices and methods for cell capture and methods of manufacture thereof

    Issued US WO2018098372A1

  • Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells

    Filed US WO2018089910A2

  • System and methods for multiplexed analysis of cellular and other immunotherapeutics

    Issued US WO2018049418A1

Courses

  • Convection Heat & Mass Transfer

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  • Fluid Dynamics

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  • Intro to Continuum Mechanics

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  • Method of Mechanical Engineering Analysis

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  • Molecular Biology of Cell

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  • Python for Everybody

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  • Statistics in Research

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Projects

  • Single Cell Multiplex Intracellular Protein Capture Device

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    Based on the academic work developed and published by Jim Heath' lab at Caltech, IsoPlexis developed a technology that is more user friendly and commercially scalable than its academic counterpart. The Isopleis consumable utilizes controlled drug released technology to lyse and capture proteins from single cells rather than utlizing active microfluidics. This improvement greatly simplified the manufacturing and commercialization process as the consumable was compatible with the existing…

    Based on the academic work developed and published by Jim Heath' lab at Caltech, IsoPlexis developed a technology that is more user friendly and commercially scalable than its academic counterpart. The Isopleis consumable utilizes controlled drug released technology to lyse and capture proteins from single cells rather than utlizing active microfluidics. This improvement greatly simplified the manufacturing and commercialization process as the consumable was compatible with the existing instrument architecture and could be mass produced with far less capital investment.

    This project required developing a unique hydrogel chemistry that could a release a detergent at the desired rate, an optimal lysis buffer formulation that was compatible with the release chemistry and sufficiently solubilized and stabilized the intracellular proteins, an immobilization chemistry that could link the loaded hydrogel to the PDMS microchambers and a unique manufacturing method that could coat the hydrogel in the microchambers and seal the device.

    A patent was issued on this technology called Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells on Nov 22, 2018 (WO2018089910A2 )

  • IsoCode Immuno-oncology Sampe Preperation Protocols for in-vitro Single Cell Analysis

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    Developed sample preperation protocols IsoCode/ SCBC technology for a large range of immunology applications including cell engineering and therapy, checkpoint and combination immunotherapy, vaccines and immunology research, inflammatory and autoimmune disease and solid tumor and oncology. These protocols were used and published in over 30 different papers and conference presentations that discovered clinical biomarkers where state of the art technologies could not.

    Protocol…

    Developed sample preperation protocols IsoCode/ SCBC technology for a large range of immunology applications including cell engineering and therapy, checkpoint and combination immunotherapy, vaccines and immunology research, inflammatory and autoimmune disease and solid tumor and oncology. These protocols were used and published in over 30 different papers and conference presentations that discovered clinical biomarkers where state of the art technologies could not.

    Protocol optimization required validation with comparator assays to verify single cells results with industry standard immunoassays such as ELISA, intracellular cytokine staining (ICS), bead based immunoassays (e.g. Luminex).
    In addition, a variety of cell selection/enrichment technologies were optimized for primary human cells including bead enrichment and flow cytometry.
    Finally, a variety of cell types such as T-cell, NK, innate, CAR-T and TCR-T were used to optimize activation to discover clinical correlates with the IsoCode technology. In-vitro stimulation with different stimulants, APCs and kinetics were explored and compared with clincical outcome

  • Larus Senior Design Project: Bio-Inspired Concepts for Aerospace Applications

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    Worked on developing a bio-inspired aircraft by studying and emulating the dynamic and structural characteristics of a flapping seagull. Airfoils were designed and modeled in ANSYS fluent to optimize the aerodynamic properties of the wing. Final airfoils were manufactured through 3D printing.

Honors & Awards

  • ASME Outstanding Paper Award

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    Awarded for the podium talk, "Zyxin Morphology, Concentration and Arrangement Modulates Force Magnitude and Directionality in fibrous Environment" at ASME NEMB Meeting in Boston, MA"

  • AIChE Best Paper in Session

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    Award for the podium talk, "Change in Cellular Behavior with the Implementation of Aligned
    PLGA Fibers in Wound Healing Model" at AIChE 2012 Meeting in Pittsburgh, PA

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