Daniele Canzani, PhD
Seattle, Washington, United States
393 followers
381 connections
Activity
-
With $11.2M in new funding led by Two Bear Capital, Talus Bio is set to revolutionize the way we approach drug discovery. Our MARMOT platform is…
With $11.2M in new funding led by Two Bear Capital, Talus Bio is set to revolutionize the way we approach drug discovery. Our MARMOT platform is…
Liked by Daniele Canzani, PhD
-
We've got an exciting announcement on Monday...
We've got an exciting announcement on Monday...
Liked by Daniele Canzani, PhD
Experience
Education
Publications
-
Degronomics: Mapping the Interacting Peptidome of a Ubiquitin Ligase Using an Integrative Mass Spectrometry Strategy
Analytical Chemsitry
"Human cells make use of hundreds of unique ubiquitin E3 ligases to ensure proteome fidelity and control cellular functions by promoting protein degradation. These processes require exquisite selectivity, but the individual roles of most E3s remain poorly characterized in part due to the challenges associated with identifying, quantifying, and validating substrates for each E3. We report an integrative mass spectrometry (MS) strategy for characterizing protein fragments that interact with…
"Human cells make use of hundreds of unique ubiquitin E3 ligases to ensure proteome fidelity and control cellular functions by promoting protein degradation. These processes require exquisite selectivity, but the individual roles of most E3s remain poorly characterized in part due to the challenges associated with identifying, quantifying, and validating substrates for each E3. We report an integrative mass spectrometry (MS) strategy for characterizing protein fragments that interact with KLHDC2, a human E3 that recognizes the extreme C-terminus of substrates. Using a combination of native MS, native top-down MS, MS of destabilized samples, and liquid chromatography MS, we identified and quantified a near complete fraction of the KLHDC2-binding peptidome in E. coli cells. This degronome includes peptides that originate from a variety of proteins. Although all identified protein fragments are terminated by diglycine or glycylalanine, the preceding amino acids are diverse. These results significantly expand our understanding of the sequences that can be recognized by KLHDC2, which provides insight into the potential substrates of this E3 in humans. We anticipate that this integrative MS strategy could be leveraged more broadly to characterize the degronomes of other E3 ligase substrate receptors, including those that adhere to the more common N-end rule for substrate recognition. Therefore, this work advances “degronomics,” i.e., identifying, quantifying, and validating functional E3:peptide interactions in order to determine the individual roles of each E3."
-
Ion Mobility of Proteins in Nitrogen Gas: Effects of Charge State, Charge Distribution, and Structure
Journal of Physical Chemistry A
-
LC-MS/MS Validation of a Residue Analysis Method for Penicillin G and its Metabolites in Commercial Orange Juice
Journal of the AOAC International
Florida citrus depends on a breakthrough in the fight against citrus greening disease. Of the antibiotics used to treat this disease, penicillin G has been one of the most effective. Because orange fruit grown in the state of Florida are mainly used to produce orange juice, we have validated an ultra-HPLC tandem MS method to screen for penicillin G and its metabolites (penillic and penilloic acids) at the chemical residue level after treatment. In this method, three spike levels (0.25, 1, and…
Florida citrus depends on a breakthrough in the fight against citrus greening disease. Of the antibiotics used to treat this disease, penicillin G has been one of the most effective. Because orange fruit grown in the state of Florida are mainly used to produce orange juice, we have validated an ultra-HPLC tandem MS method to screen for penicillin G and its metabolites (penillic and penilloic acids) at the chemical residue level after treatment. In this method, three spike levels (0.25, 1, and 20 ng/g) were tested in triplicate. Absolute recoveries for penillic and penilloic acids were 60–75% depending on the matrix used, whereas corrected recoveries of penicillin G using an isotopically labeled internal standard were ~100%. Two product ion transitions per analyte were required for identification, which contributes to a high degree of selectivity.
Other authorsSee publication -
UHPLC-MS/MS Analysis of Penicillin G and its Major Metabolites in Citrus
LCGC North America
In recent years, citrus greening disease, also known as huanglongbing
(HLB), has devastated citrus crops throughout the world. Penicillin
G has been used to treat HLB-infected trees with promising
results. However, the metabolites produced from the degradation
of penicillin G are known to cause potentially life-threatening
allergic reactions. Therefore, the concentration and presence of
the metabolites must be carefully monitored. We have developed
and revised an analytical…In recent years, citrus greening disease, also known as huanglongbing
(HLB), has devastated citrus crops throughout the world. Penicillin
G has been used to treat HLB-infected trees with promising
results. However, the metabolites produced from the degradation
of penicillin G are known to cause potentially life-threatening
allergic reactions. Therefore, the concentration and presence of
the metabolites must be carefully monitored. We have developed
and revised an analytical method based on ultrahigh-pressure
liquid chromatography (UHPLC) in combination with tandem mass
spectrometry (MS/MS) to identify and quantitate penicillin G and its
major metabolites, penillic acid and penilloic acid, in citrus fruit and
juice. Here, we discuss the chromatographic conditions and revisions
that improved the precision and accuracy of our measurements.Other authors -
UHPLC-MS/MS Method for the Quantitation of Penicillin G and Metabolites in Citrus Fruit using Internal Standards
Journal of Chromatography B
Penicillin G has been applied to citrus trees as a potential treatment in the fight against Huanglongbing (HLB). Here, we have developed and validated a method to identify and quantitate penicillin G and two of its metabolites, penillic acid and penilloic acid, in citrus fruit using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). This method improves upon a previous method by incorporating isotopically labeled internal standards, namely, penillic acid-D 5…
Penicillin G has been applied to citrus trees as a potential treatment in the fight against Huanglongbing (HLB). Here, we have developed and validated a method to identify and quantitate penicillin G and two of its metabolites, penillic acid and penilloic acid, in citrus fruit using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). This method improves upon a previous method by incorporating isotopically labeled internal standards, namely, penillic acid-D 5, and penilloic acid-D 5. These standards greatly enhanced the accuracy and precision of our measurements by compensating for recovery losses, degradation, and matrix effects. When 2g of citrus fruit sample is extracted, the limits of detection (LOD) were determined to be 0.1ng/g for penicillin G and penilloic acid, and 0.25ng/g for penillic acid. At fortification levels of 0.1, 0.25, 1, and 10ng/g, absolute recoveries for penillic and penilloic acids were generally between 50-70%. Recoveries corrected with the isotopically labeled standards were approximately 90-110%. This method will be useful for the identification and quantitation of drug residues and their degradation products using isotopically labeled standards and UHPLC-MS/MS.
Other authorsSee publication -
Identification of Penicillin G Metabolites under Various Environmental Conditions using UHPLC-MS/MS
Journal of Agricultural and Food Chemistry
In this work, we investigate the stability of penicillin G in various conditions including acidic, alkaline, natural acidic matrix, and after treatment of citrus trees that are infected with the citrus greening disease. The identification, confirmation, and quantitation of penicillin G and its various metabolites were evaluated using two UHPLC-MS/MS systems with variable capabilities (i.e. Thermo Q Exactive Orbitrap and Sciex 6500 Qtrap). Our data show that under acidic and alkaline conditions,…
In this work, we investigate the stability of penicillin G in various conditions including acidic, alkaline, natural acidic matrix, and after treatment of citrus trees that are infected with the citrus greening disease. The identification, confirmation, and quantitation of penicillin G and its various metabolites were evaluated using two UHPLC-MS/MS systems with variable capabilities (i.e. Thermo Q Exactive Orbitrap and Sciex 6500 Qtrap). Our data show that under acidic and alkaline conditions, penicillin G at 100 ng/mL degrades fast, with a determined half-life time of approximately 2 hours. Penillic acid, penicilloic acid, and penilloic acid are found to be the most abundant metabolites of penicillin G. These major metabolites, along with isopenillic acid, are found when penicillin G is used for treatment of the citrus greening infected trees. The findings of this study will provide insight regarding penicillin G residues for food safety purposes, in agricultural and biological applications.
Other authors
Honors & Awards
-
American Chemical Society Division of Analytical Chemistry Fellowship
American Chemical Society
-
Mickey and Karen Schurr Graduate Student Merit Fellowship
UW Department of Chemistry
-
National Institutes of Health Molecular Biophysics Training Fellow
National Institute of Health
-
Lewis R. and Joan M. Honnen Excellence in Chemistry Graduate Research Fellowship
UW Department of Chemistry
-
Scientific Day Speaker Award
FDACS Bureau of Chemical Residues
-
Undergraduate Researcher of the Year
FSU Department of Chemistry and Biochemistry
More activity by Daniele
-
We’re pleased to announce that clinical development is underway for MTX‑101, our novel autoimmune checkpoint inhibitor! The Phase 1a/b clinical study…
We’re pleased to announce that clinical development is underway for MTX‑101, our novel autoimmune checkpoint inhibitor! The Phase 1a/b clinical study…
Liked by Daniele Canzani, PhD
-
Calvin Leonen is a Filipino-American postdoctoral scientist from the island of Guam. Calvin's research in the Funabiki lab centers around the…
Calvin Leonen is a Filipino-American postdoctoral scientist from the island of Guam. Calvin's research in the Funabiki lab centers around the…
Liked by Daniele Canzani, PhD
-
🚀 I'm thrilled to announce I've embarked on a new adventure with Tabs, focusing on revolutionizing the Accounts Receivable space! Diving into the…
🚀 I'm thrilled to announce I've embarked on a new adventure with Tabs, focusing on revolutionizing the Accounts Receivable space! Diving into the…
Liked by Daniele Canzani, PhD
-
Proud to be a part of this hardworking team and Arena BioWorks exciting mission!
Proud to be a part of this hardworking team and Arena BioWorks exciting mission!
Liked by Daniele Canzani, PhD
-
🔥 Icosavax, Inc. being acquired by AstraZeneca for $1.1B!🔥 Icosavax's story is one of a groundbreaking technology being carried forward by an…
🔥 Icosavax, Inc. being acquired by AstraZeneca for $1.1B!🔥 Icosavax's story is one of a groundbreaking technology being carried forward by an…
Liked by Daniele Canzani, PhD
-
"I like to think of [proteomics] not as a big data science approach, but as the technology of choice to perform mechanistic biological studies – a…
"I like to think of [proteomics] not as a big data science approach, but as the technology of choice to perform mechanistic biological studies – a…
Liked by Daniele Canzani, PhD
-
Grateful for this unique opportunity!
Grateful for this unique opportunity!
Liked by Daniele Canzani, PhD
-
We believe "nothing is undruggable." Thank you to Michael Gibney and PharmaVoice for highlighting our mission to open up new areas of therapeutics…
We believe "nothing is undruggable." Thank you to Michael Gibney and PharmaVoice for highlighting our mission to open up new areas of therapeutics…
Liked by Daniele Canzani, PhD
-
#TPD #phase1 #publication #proteomics Nature Portfolio's Nature Medicine Successful Phase 1 clinical trial from our lead program, KT-474…
#TPD #phase1 #publication #proteomics Nature Portfolio's Nature Medicine Successful Phase 1 clinical trial from our lead program, KT-474…
Liked by Daniele Canzani, PhD
-
We were lucky enough to work with Maneeshika Madduri as one of our venture analysts. We celebrated her time with us at Washington Research…
We were lucky enough to work with Maneeshika Madduri as one of our venture analysts. We celebrated her time with us at Washington Research…
Liked by Daniele Canzani, PhD
-
Having three weeks to step back and focus on personal development is one of the greatest professional opportunities I've ever had. All of this was…
Having three weeks to step back and focus on personal development is one of the greatest professional opportunities I've ever had. All of this was…
Liked by Daniele Canzani, PhD
-
I am excited to share that I have joined leadership at Nucleate Seattle to support the expansion of trainee-led biotech innovation in the Pacific…
I am excited to share that I have joined leadership at Nucleate Seattle to support the expansion of trainee-led biotech innovation in the Pacific…
Liked by Daniele Canzani, PhD
-
Proud to be part of this team! @Talus Bio just received another $4.3M in grant funding to advance treatments for previously undruggable transcription…
Proud to be part of this team! @Talus Bio just received another $4.3M in grant funding to advance treatments for previously undruggable transcription…
Shared by Daniele Canzani, PhD
Other similar profiles
Explore collaborative articles
We’re unlocking community knowledge in a new way. Experts add insights directly into each article, started with the help of AI.
Explore More