David G Kugler
Seattle, Washington, United States
2K followers
500+ connections
Activity
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I am excited to announce that I have joined the External Innovation team at Lilly, where I will be focusing on our Immunology BD and Partnering…
I am excited to announce that I have joined the External Innovation team at Lilly, where I will be focusing on our Immunology BD and Partnering…
Liked by David G Kugler
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More big news from ShapeTX today - we are bringing two world-class BioPharma execs to our Board of Directors! These additions will help guide the…
More big news from ShapeTX today - we are bringing two world-class BioPharma execs to our Board of Directors! These additions will help guide the…
Liked by David G Kugler
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Nearly six years ago, we joined the #FlagshipFounded ecosystem, paving the way for Flagship Pioneering’s #AI portfolio and kickstarting the…
Nearly six years ago, we joined the #FlagshipFounded ecosystem, paving the way for Flagship Pioneering’s #AI portfolio and kickstarting the…
Liked by David G Kugler
Volunteer Experience
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Emergency Room Volunteer
Harborview Medical Center
- 2 years
Health
ER volunteer position prepping rooms and organizing supplies, with 200+ hours of direct patient contact and observation. Seattle, WA.
Publications
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Systemic toxoplasma infection triggers a long-term defect in the generation and function of naive T lymphocytes
Journal of Experimental Medicine
Because antigen-stimulated naive T cells either die as effectors or enter the activated/memory pool, continuous egress of new T lymphocytes from thymus is essential for maintenance of peripheral immune homeostasis. Unexpectedly, we found that systemic infection with the protozoan Toxoplasma gondii triggers not only a transient (temporary) increase in activated CD4+ Th1 cells but also a persistent decrease in the size of the naive CD4+ T lymphocyte pool. This immune defect is associated with…
Because antigen-stimulated naive T cells either die as effectors or enter the activated/memory pool, continuous egress of new T lymphocytes from thymus is essential for maintenance of peripheral immune homeostasis. Unexpectedly, we found that systemic infection with the protozoan Toxoplasma gondii triggers not only a transient (temporary) increase in activated CD4+ Th1 cells but also a persistent decrease in the size of the naive CD4+ T lymphocyte pool. This immune defect is associated with decreased thymic output and parasite-induced destruction of the thymic epithelium, as well as disruption of the overall architecture of that primary lymphoid organ. Importantly, the resulting quantitative and qualitative deficiency in naive CD4+ T cells leads to an immunocompromised state that both promotes chronic toxoplasma infection and leads to decreased resistance to challenge with an unrelated pathogen. These findings reveal that systemic infectious agents, such as T. gondii, can induce long-term immune alterations associated with impaired thymic function. When accumulated during the lifetime of the host, such events, even when occurring at low magnitude, could be a contributing factor in immunological senescence.
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miR-155 activates cytokine gene expression in Th17 cells by regulating the DNA-binding protein Jarid2 to relieve polycomb-mediated repression.
Immunity
Specification of the T helper 17 (Th17) cell lineage requires a well-defined set of transcription factors, but how these integrate with posttranscriptional and epigenetic programs to regulate gene expression is poorly understood. Here we found defective Th17 cell cytokine expression in miR-155-deficient CD4+ T cells in vitro and in vivo. Mir155 was bound by Th17 cell transcription factors and was highly expressed during Th17 cell differentiation. miR-155-deficient Th17 and T regulatory (Treg)…
Specification of the T helper 17 (Th17) cell lineage requires a well-defined set of transcription factors, but how these integrate with posttranscriptional and epigenetic programs to regulate gene expression is poorly understood. Here we found defective Th17 cell cytokine expression in miR-155-deficient CD4+ T cells in vitro and in vivo. Mir155 was bound by Th17 cell transcription factors and was highly expressed during Th17 cell differentiation. miR-155-deficient Th17 and T regulatory (Treg) cells expressed increased amounts of Jarid2, a DNA-binding protein that recruits the Polycomb Repressive Complex 2 (PRC2) to chromatin. PRC2 binding to chromatin and H3K27 histone methylation was increased in miR-155-deficient cells, coinciding with failure to express Il22, Il10, Il9, and Atf3. Defects in Th17 cell cytokine expression and Treg cell homeostasis in the absence of Mir155 could be partially suppressed by Jarid2 deletion. Thus, miR-155 contributes to Th17 cell function by suppressing the inhibitory effects of Jarid2.
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CD4+ T cells are trigger and target of the glucocorticoid response that prevents lethal immunopathology in toxoplasma infection
Brief Definitive Report, Journal of Experimental Medicine
Synthetic glucocorticoids (GCs) are commonly used in the treatment of inflammatory diseases, but the role of endogenous GCs in the regulation of host-protective immune responses is poorly understood. Here we show that GCs are induced during acute Toxoplasma gondii infection and directly control the T cell response to the parasite. When infected with toxoplasma, mice that selectively lack GC receptor (GR) expression in T cells (GRlck-Cre) rapidly succumb to infection despite displaying parasite…
Synthetic glucocorticoids (GCs) are commonly used in the treatment of inflammatory diseases, but the role of endogenous GCs in the regulation of host-protective immune responses is poorly understood. Here we show that GCs are induced during acute Toxoplasma gondii infection and directly control the T cell response to the parasite. When infected with toxoplasma, mice that selectively lack GC receptor (GR) expression in T cells (GRlck-Cre) rapidly succumb to infection despite displaying parasite burdens indistinguishable from control animals and unaltered levels of the innate cytokines IL-12 and IL-27. Mortality in the GRlck-Cre mice was associated with immunopathology and hyperactive Th1 cell function as revealed by enhanced IFN-γ and TNF production in vivo. Unexpectedly, these CD4+ T lymphocytes also overexpressed IL-10. Importantly, CD4+ T cell depletion in wild-type or GRlck-Cre mice led to ablation of the GC response to infection. Moreover, in toxoplasma-infected RAG−/− animals, adoptive transfer of CD4+ T lymphocytes was required for GC induction. These findings establish a novel IL-10–independent immunomodulatory circuit in which CD4+ T cells trigger a GC response that in turn dampens their own effector function. In the case of T. gondii infection, this self-regulatory pathway is critical for preventing collateral tissue damage and promoting host survival.
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Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities
Journal of Immunology
IL-25 (IL-17E) is a unique IL-17 family ligand that promotes Th2-skewed inflammatory responses. Intranasal administration of IL-25 into naive mice induces pulmonary inflammation similar to that seen in patients with allergic asthma, including increases in bronchoalveolar lavage fluid eosinophils, bronchoalveolar lavage fluid IL-5 and IL-13 concentrations, goblet cell hyperplasia, and increased airway hyperresponsiveness. IL-25 has been reported to bind and signal through IL-17RB (IL-17BR…
IL-25 (IL-17E) is a unique IL-17 family ligand that promotes Th2-skewed inflammatory responses. Intranasal administration of IL-25 into naive mice induces pulmonary inflammation similar to that seen in patients with allergic asthma, including increases in bronchoalveolar lavage fluid eosinophils, bronchoalveolar lavage fluid IL-5 and IL-13 concentrations, goblet cell hyperplasia, and increased airway hyperresponsiveness. IL-25 has been reported to bind and signal through IL-17RB (IL-17BR, IL-17Rh1). It has been demonstrated recently that IL-17A signals through a heteromeric receptor composed of IL-17RA and IL-17RC. We sought to determine whether other IL-17 family ligands also utilize heteromeric receptor complexes. The required receptor subunits for IL-25 biological activities were investigated in vitro and in vivo using a combination of knockout (KO) mice and antagonistic Abs. Unlike wild-type mice, cultured splenocytes from either IL-17RB KO or IL-17RA KO mice did not produce IL-5 or IL-13 in response to IL-25 stimulation, and both IL-17RB KO and IL-17RA KO mice did not respond to intranasal administration of IL-25. Furthermore, treatment with antagonistic mAbs to either IL-17RB or IL-17RA completely blocked IL-25-induced pulmonary inflammation and airway hyperresponsiveness in naive BALB/c mice, similar to the effects of an antagonistic Ab to IL-25. Finally, a blocking Ab to human IL-17RA prevented IL-25 activity in a primary human cell-based assay. These data demonstrate for the first time that IL-25-mediated activities require both IL-17RB and IL-17RA and provide another example of an IL-17 family ligand that utilizes a heteromeric receptor complex.
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Opposing activities of two novel members of the IL-1 ligand family regulate skin inflammation
Journal of Experimental Medicine
More activity by David G
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We’re hiring! Join us as a Principal Scientist, Machine Learning/Predictive Cell State Design and use your expertise in machine learning to develop…
We’re hiring! Join us as a Principal Scientist, Machine Learning/Predictive Cell State Design and use your expertise in machine learning to develop…
Liked by David G Kugler
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Cartography CEO Kevin Parker, Ph.D., recently sat down with the team at Alix Ventures' BIOS Builders podcast to share insight into our precision…
Cartography CEO Kevin Parker, Ph.D., recently sat down with the team at Alix Ventures' BIOS Builders podcast to share insight into our precision…
Liked by David G Kugler
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Did you know that the Seattle area is ranked as a top growing market for life sciences talent? Read more about why top talent is coming to the…
Did you know that the Seattle area is ranked as a top growing market for life sciences talent? Read more about why top talent is coming to the…
Liked by David G Kugler
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I am so honored to be participating in this year’s Bristol Myers Squibb’s Coast 2 Coast 4 Cancer ride. Over the course of a month, BMS employees will…
I am so honored to be participating in this year’s Bristol Myers Squibb’s Coast 2 Coast 4 Cancer ride. Over the course of a month, BMS employees will…
Liked by David G Kugler
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Inspiring developments today from Flagship Pioneering with a $3.6 billion raise to drive groundbreaking innovations in health and sustainability. As…
Inspiring developments today from Flagship Pioneering with a $3.6 billion raise to drive groundbreaking innovations in health and sustainability. As…
Liked by David G Kugler
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Research led by 2023 Parker Scholar Chris McGinnis, PhD, Stanford University School of Medicine, and PICI Investigator Ansuman Satpathy, MD, PhD…
Research led by 2023 Parker Scholar Chris McGinnis, PhD, Stanford University School of Medicine, and PICI Investigator Ansuman Satpathy, MD, PhD…
Liked by David G Kugler
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'NIH researchers achieved tumor shrinkage in three of seven patients with colorectal cancers.'
'NIH researchers achieved tumor shrinkage in three of seven patients with colorectal cancers.'
Liked by David G Kugler
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Researchers led by Drs. Nitin Baliga and James Park at the Institute for Systems Biology (ISB) looked at transcriptional regulatory networks to…
Researchers led by Drs. Nitin Baliga and James Park at the Institute for Systems Biology (ISB) looked at transcriptional regulatory networks to…
Liked by David G Kugler
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