About
Cell therapy translation, development, and manufacturing leader.
Specialties: Team…
Activity
-
I have retired from my long career in biotech and cell and gene therapy Qualility Assurance. We are traveling and enjoying seeing this beautiful…
I have retired from my long career in biotech and cell and gene therapy Qualility Assurance. We are traveling and enjoying seeing this beautiful…
Liked by Ian Gaudet
-
My connection to the foundation began 8-9 years ago while at Hanson Wade Group's CAR-TCR Summit in Boston. There wasn't a dry eye in the house after…
My connection to the foundation began 8-9 years ago while at Hanson Wade Group's CAR-TCR Summit in Boston. There wasn't a dry eye in the house after…
Liked by Ian Gaudet
Experience
Education
-
Rutgers, The State University of New Jersey-New Brunswick
-
Activities and Societies: Bioengineering Student Society, AAAS, Society for Biomaterials, Biomedical Engineering Society
-
-
ABET
Publications
-
Methacrylation Induces Rapid, Temperature-Dependent, Reversible Self-Assembly of Type-I Collagen
Langmuir
Type-I collagen self-assembles into a fibrillar gel at physiological temperature and pH to provide a cell-adhesive, supportive, structural network. As such, it is an attractive, popular scaffold for in vitro evaluations of cellular behavior and for tissue engineering applications. In this study, type-I collagen is modified to introduce methacrylate groups on the free amines of the lysine residues to create collagen methacrylamide (CMA). CMA retains the properties of collagen such as…
Type-I collagen self-assembles into a fibrillar gel at physiological temperature and pH to provide a cell-adhesive, supportive, structural network. As such, it is an attractive, popular scaffold for in vitro evaluations of cellular behavior and for tissue engineering applications. In this study, type-I collagen is modified to introduce methacrylate groups on the free amines of the lysine residues to create collagen methacrylamide (CMA). CMA retains the properties of collagen such as self-assembly, biodegradability, and natural bioactivity but is also photoactive and can be rapidly cross-linked or functionalized with acrylated molecules when irradiated with ultraviolet light in the presence of a photoinitiator. CMA also demonstrates unique temperature-dependent behavior. For natural type-I collagen, the overall structure of the fiber network remains largely static over time scales of a few hours upon heating and cooling at temperatures below its denaturation point. CMA, however, is rapidly thermoreversible and will oscillate between a liquid macromer suspension and a semisolid fibrillar hydrogel when the temperature is modulated between 10 and 37 °C. Using a series of mechanical, scattering, and spectroscopic methods, we demonstrate that structural reversibility is manifest across multiple scales from the protein topology of the triple helix up through the rheological properties of the CMA hydrogel. Electron microscopy imaging of CMA after various stages of heating and cooling shows that the canonical collagen-like D-periodic banding ultrastructure of the fibers is preserved. A rapidly thermoreversible collagen-based hydrogel is expected to have wide utility in tissue engineering and drug delivery applications as a biofunctional, biocompatible material. Thermal reversibility also makes CMA a powerful model for studying the complex process of hierarchical collagen self-assembly.
Other authors -
Characterization of methacrylated type-I collagen as a dynamic, photoactive hydrogel
Biointerphases
Type-I collagen is an attractive scaffold material for tissue engineering due to its ability to self-assemble into a fibrillar hydrogel, its innate support of tissue cells through bioactive adhesion sites, and its biodegradability. However, a lack of control of material properties has hampered its utility as a scaffold. We have modified collagen via the addition of methacrylate groups to create collagen methacrylamide (CMA) using a synthesis reaction that allows retention of fundamental…
Type-I collagen is an attractive scaffold material for tissue engineering due to its ability to self-assemble into a fibrillar hydrogel, its innate support of tissue cells through bioactive adhesion sites, and its biodegradability. However, a lack of control of material properties has hampered its utility as a scaffold. We have modified collagen via the addition of methacrylate groups to create collagen methacrylamide (CMA) using a synthesis reaction that allows retention of fundamental characteristics of native collagen, including spontaneous fibrillar self-assembly and enzymatic biodegradability. This method allows for a rapid, five-fold increase in storage modulus upon irradiation with 365 nm light. Fibrillar diameter of CMA was not significantly different from native collagen. Collagenolytic degradability of uncrosslinked CMA was minimally reduced, while photocrosslinked CMA was significantly more resistant to degradation. Live/Dead staining demonstrated that a large majority (71%) of encapsulated mesenchymal stem cells remained viable 24 h after photocrosslinking, which further increased to 81% after 72 h. This material represents a novel platform for creating mechanically heterogeneous environments.
Other authorsSee publication
Patents
-
Process for the synthesis of methacrylate-derivatized type-1 collagen and derivatives thereof
Issued USPTO 08658711
Methods for synthesizing a methacrylate-derivatized type-I collagen in which methacrylic acid is reacted with a carboxylic acid activating reagent in the presence of a carbodiimide to form a methacrylic acid with an activated carboxylic acid group, which is then reacted with free amino groups on type-I collagen to form a collagen methacrylamide. Methacrylate-derivatized collagen, cross-linked collagens formed therefrom and products containing the cross-linked collagen are also disclosed.
Other inventorsSee patent
Courses
-
Bioengineering Design
BENG 186C
-
Bioengineering Laboratory
BENG 172
-
Bioengineering Mass Transport
BENG 103B
-
Bioengineering Physiology
BENG 140 A&B
-
Biomaterials and Biomechanics
16125582
-
Biomechanics
MAE 112A&B
-
Biomedical Electronics
BENG 122B
-
Biosignal Processing
16125571
-
Biosystems and Control
BENG 122A
-
Cell and Tissue Engineering
BENG 166A
-
Clinical Practicum
16125628
-
Computational Methods
MAE 107
-
Computer-Aided Design
MAE 150
-
Continuum Mechanics
BENG 110
-
Critical Writing
MCWP 50
-
Ethical Scientific Conduct
16115556
-
Fluid Mechanics
CENG 101A
-
Fortran for Engineers
AMES 10
-
Journal Club
16125601
-
Kinetics Thermodynamics and Transport
16125573
-
Linear Algebra
MATH 20F
-
Linear Circuits
MAE 140
-
Mammalian Physiology
16125581
-
Mechanics I: Statics
AMES 121A
-
Mechanics II: Dynamics
AMES 121B
-
Methods in Applied Mathematics
16642527
-
Molecular Biology and Biochemistry
16115511
-
Molecular Physical Chemistry
BENG 130
-
Neural Injury and Repair
16963632
-
Polymer Science and Engineering
16155551
-
Principles of Bioinstrumentation
BENG 186B
-
Principles of Biomaterials
BENG 186A
-
Stem Cell Biology and Engineering
16125586
-
Vector Calculus
MATH 20E
More activity by Ian
-
Thrilled and honored to receive my first @NIH R01 grant to explore the role of IFN1 signaling in non-activated CAR T cells. Huge thanks to NCI for…
Thrilled and honored to receive my first @NIH R01 grant to explore the role of IFN1 signaling in non-activated CAR T cells. Huge thanks to NCI for…
Liked by Ian Gaudet
-
The starting material used in cell therapies and gene-modified cell therapies has a huge impact on logistics, manufacturability/COGs, and regulatory…
The starting material used in cell therapies and gene-modified cell therapies has a huge impact on logistics, manufacturability/COGs, and regulatory…
Liked by Ian Gaudet
-
Help if you can spare it... News no parent wants to hear ... Share this post if you can.
Help if you can spare it... News no parent wants to hear ... Share this post if you can.
Liked by Ian Gaudet
-
Adaptimmune Gears Up for Potential Approval of First-in-Class Engineered T Cell Therapy After more than a decade devoid of therapeutic advancements,…
Adaptimmune Gears Up for Potential Approval of First-in-Class Engineered T Cell Therapy After more than a decade devoid of therapeutic advancements,…
Liked by Ian Gaudet
-
The #FDA has released the latest draft guidance on the use of Human- and Animal-Derived Materials in Cellular and Gene Therapy and Tissue-Engineered…
The #FDA has released the latest draft guidance on the use of Human- and Animal-Derived Materials in Cellular and Gene Therapy and Tissue-Engineered…
Liked by Ian Gaudet
-
Grateful for the Wall Street Journal highlighting our work to accelerate discovery of the critical metals for energy transition
Grateful for the Wall Street Journal highlighting our work to accelerate discovery of the critical metals for energy transition
Liked by Ian Gaudet
-
I'm hiring a Lab Manager for our Irvine operations. If you know of any good candidates, please send them our way! #hiring Karin Koch Barbara (Nsiah)…
I'm hiring a Lab Manager for our Irvine operations. If you know of any good candidates, please send them our way! #hiring Karin Koch Barbara (Nsiah)…
Liked by Ian Gaudet
Other similar profiles
Explore collaborative articles
We’re unlocking community knowledge in a new way. Experts add insights directly into each article, started with the help of AI.
Explore More