Jared Kehe
Cambridge, Massachusetts, United States
1K followers
500+ connections
About
Concerto Biosciences examines the microbial world with unmatched experimental depth to…
Activity
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Last week, along with some collegues from Grupo Bimbo, I traveled to the United States to meet with various highly disruptive #startups. In Boston…
Last week, along with some collegues from Grupo Bimbo, I traveled to the United States to meet with various highly disruptive #startups. In Boston…
Liked by Jared Kehe
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My first first-author research paper published in Cell ❤️ Thanks to all our wonderful collaborators, peer reviewers, and the editorial staff!
My first first-author research paper published in Cell ❤️ Thanks to all our wonderful collaborators, peer reviewers, and the editorial staff!
Liked by Jared Kehe
Experience
Education
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Massachusetts Institute of Technology
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Activities and Societies: MIT Microbiome Club President; Biological Engineering Graduate Student Board; MIT Graduate Student Blog Copy Co-Chief
NSF Graduate Fellow, Siebel Scholar, Maslak Presidential Graduate Fellow, Deshpande Center for Technological Innovation Grantee
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Activities and Societies: Tau Beta Pi; Biomedical Engineering Society; International Society for Pharmaceutical Engineers; Caledonian Society
Graduated summa cum laude; Muir Scholar; Provost Honors; Award for Excellence and Leadership and Service, Bioengineering Department
Volunteer Experience
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Instructor
Science Club for Girls
- 3 months
Education
Constructed full curriculum and ran 1-week microbiology course for underprivileged girls (intermediate school and high school)
Publications
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Positive interactions are common among culturable bacteria
Science Advances
Interspecies interactions shape the structure and function of microbial communities. In particular, positive, growth-promoting interactions can substantially affect the diversity and productivity of natural and engineered communities. However, the prevalence of positive interactions and the conditions in which they occur are not well understood. To address this knowledge gap, we used kChip, an ultrahigh-throughput coculture platform, to measure 180,408 interactions among 20 soil bacteria across…
Interspecies interactions shape the structure and function of microbial communities. In particular, positive, growth-promoting interactions can substantially affect the diversity and productivity of natural and engineered communities. However, the prevalence of positive interactions and the conditions in which they occur are not well understood. To address this knowledge gap, we used kChip, an ultrahigh-throughput coculture platform, to measure 180,408 interactions among 20 soil bacteria across 40 carbon environments. We find that positive interactions, often described to be rare, occur commonly and primarily as parasitisms between strains that differ in their carbon consumption profiles. Notably, nongrowing strains are almost always promoted by strongly growing strains (85%), suggesting a simple positive interaction–mediated approach for cultivation, microbiome engineering, and microbial consortium design.
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Massively parallel screening of synthetic microbial communities
PNAS
Microbial communities have many applications, but current experimental strategies to investigate their behavior are limited by the combinatorial complexity of interactions between species. Here, we introduce a platform to automatically construct and test synthetic communities of microbes from a set of input species at a scale of ∼100,000 communities per day. As a first demonstration, we discovered specific compositions of bacteria isolated from local soil that promote the growth of a model…
Microbial communities have many applications, but current experimental strategies to investigate their behavior are limited by the combinatorial complexity of interactions between species. Here, we introduce a platform to automatically construct and test synthetic communities of microbes from a set of input species at a scale of ∼100,000 communities per day. As a first demonstration, we discovered specific compositions of bacteria isolated from local soil that promote the growth of a model plant symbiont. More broadly, our platform can be adopted for the discovery of microbial consortia with many useful properties, such as suppression of pathogens or degradation of recalcitrant substrates for use in biofuel production or environmental remediation.
Other authorsSee publication -
Combinatorial drug discovery in nanoliter droplets
PNAS
The conventional single-agent approach to therapeutics is challenged by the redundancy and feedback in biological networks. The use of multiple drugs in combination might improve desired functional outcomes while reducing toxicity and overcoming drug resistance. However, the complexity and resources required to test many combinations have slowed discovery efforts. Here, we introduce a screening platform that automatically constructs combinations of drugs from nanoliter-scale droplets, greatly…
The conventional single-agent approach to therapeutics is challenged by the redundancy and feedback in biological networks. The use of multiple drugs in combination might improve desired functional outcomes while reducing toxicity and overcoming drug resistance. However, the complexity and resources required to test many combinations have slowed discovery efforts. Here, we introduce a screening platform that automatically constructs combinations of drugs from nanoliter-scale droplets, greatly simplifying screening logistics and reducing resource consumption. We applied our platform to discover drug pairs that act synergistically against the model pathogen Escherichia coli. Our platform can be further developed to support many types of phenotypic assays in a variety of disease models.
Other authorsSee publication -
High content image analysis of focal adhesion-dependent mechanosensitive stem cell differentiation
Integrative Biology
Human mesenchymal stem cells (hMSCs) receive differentiation cues from a number of stimuli, including extracellular matrix (ECM) stiffness. The pathways used to sense stiffness and other physical cues are just now being understood and include proteins within focal adhesions. To rapidly advance the pace of discovery for novel mechanosensitive proteins, we employed a combination of in silico and high throughput in vitro methods to analyze 47 different focal adhesion proteins for cryptic kinase…
Human mesenchymal stem cells (hMSCs) receive differentiation cues from a number of stimuli, including extracellular matrix (ECM) stiffness. The pathways used to sense stiffness and other physical cues are just now being understood and include proteins within focal adhesions. To rapidly advance the pace of discovery for novel mechanosensitive proteins, we employed a combination of in silico and high throughput in vitro methods to analyze 47 different focal adhesion proteins for cryptic kinase binding sites. High content imaging of hMSCs treated with small interfering RNAs for the top 6 candidate proteins showed novel effects on both osteogenic and myogenic differentiation; Vinculin and SORBS1 were necessary for stiffness-mediated myogenic and osteogenic differentiation, respectively. Both of these proteins bound to MAPK1 (also known as ERK2), suggesting that it plays a context-specific role in mechanosensing for each lineage; validation for these sites was performed. This high throughput system, while specifically built to analyze stiffness-mediated stem cell differentiation, can be expanded to other physical cues to more broadly assess mechanical signaling and increase the pace of sensor discovery.
More activity by Jared
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On the latest episode of the Building Biotechs podcast, hosted by Carina Clingman, PhD, our CEO Cheri Ackerman shares Concerto’s perspective on…
On the latest episode of the Building Biotechs podcast, hosted by Carina Clingman, PhD, our CEO Cheri Ackerman shares Concerto’s perspective on…
Liked by Jared Kehe
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The first time I met President Biden was in (approximately) 1999, when for some reason he was visiting Missouri, doing a ribbon-cutting for a women's…
The first time I met President Biden was in (approximately) 1999, when for some reason he was visiting Missouri, doing a ribbon-cutting for a women's…
Liked by Jared Kehe
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A few days ago, I performed a very special miniprep. Not only was I training two new computer scientists on the basic wet-lab protocol (a rite of…
A few days ago, I performed a very special miniprep. Not only was I training two new computer scientists on the basic wet-lab protocol (a rite of…
Liked by Jared Kehe
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Wondering how Concerto thinks about AI, global health, the looming threat of antibiotic resistance, and how the kChip discovery platform fits into…
Wondering how Concerto thinks about AI, global health, the looming threat of antibiotic resistance, and how the kChip discovery platform fits into…
Liked by Jared Kehe
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This milestone is a testament to the incredible dedication, innovation, and hard work of the entire team. Every challenge we faced was met with…
This milestone is a testament to the incredible dedication, innovation, and hard work of the entire team. Every challenge we faced was met with…
Liked by Jared Kehe
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Big clinical-trial news today from Concerto Biosciences! Congratulations Cheri Ackerman, Jared Kehe, Bernardo Cervantes and the entire Concerto team…
Big clinical-trial news today from Concerto Biosciences! Congratulations Cheri Ackerman, Jared Kehe, Bernardo Cervantes and the entire Concerto team…
Liked by Jared Kehe
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Microbial therapeutics will be big and impactful. Congrats Concerto Biosciences! 👏
Microbial therapeutics will be big and impactful. Congrats Concerto Biosciences! 👏
Liked by Jared Kehe
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