“Justin is an outstanding, no nonsense scientist and engineer. He has a great aptitude for detailed work and very much prioritizes understanding the fundamental science behind his projects. He quickly distills and employs new information to meet project goals and seeks the most direct route to viable solutions. He would make a great addition to any scientific or business team, and I would gladly work with Justin again.”
Justin Koepsel, PhD, MBA
Madison, Wisconsin, United States
2K followers
500+ connections
About
Leader with a proven track record of performance at leading biomedical companies…
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🎉 Big News for Wisconsin! 🎉 On July 22, Gov. Tony Evers and U.S. Sen. Tammy Baldwin visited Milwaukee Area Technical College (MATC) to celebrate…
🎉 Big News for Wisconsin! 🎉 On July 22, Gov. Tony Evers and U.S. Sen. Tammy Baldwin visited Milwaukee Area Technical College (MATC) to celebrate…
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Today we celebrated the ribbon cutting for Mayo Clinic in Florida’s new Bill Hewitt Employee Wellness Center, a state-of-the-art facility that will…
Today we celebrated the ribbon cutting for Mayo Clinic in Florida’s new Bill Hewitt Employee Wellness Center, a state-of-the-art facility that will…
Liked by Justin Koepsel, PhD, MBA
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⭐️ Promega is #Hiring in the Tennessee area! ⭐️ We are on the hunt for a Senior Client Representative to join the best sales team (humble…
⭐️ Promega is #Hiring in the Tennessee area! ⭐️ We are on the hunt for a Senior Client Representative to join the best sales team (humble…
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Experience
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Publications
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Biomaterial arrays with defined adhesion ligand densities and matrix stiffness identify distinct phenotypes for tumorigenic and non-tumorigenic human mesenchymal cell types
Biomaterials Science
Here, we aimed to investigate migration of a model tumor cell line (HT-1080 fibrosarcoma cells, HT-1080s) using synthetic biomaterials to systematically vary peptide ligand density and substrate stiffness. A range of substrate elastic moduli were investigated by using poly(ethylene glycol) (PEG) hydrogel arrays (0.34–17 kPa) and self-assembled monolayer (SAM) arrays ([similar]0.1–1 GPa), while cell adhesion was tuned by varying the presentation of Arg-Gly-Asp (RGD)-containing peptides. HT-1080…
Here, we aimed to investigate migration of a model tumor cell line (HT-1080 fibrosarcoma cells, HT-1080s) using synthetic biomaterials to systematically vary peptide ligand density and substrate stiffness. A range of substrate elastic moduli were investigated by using poly(ethylene glycol) (PEG) hydrogel arrays (0.34–17 kPa) and self-assembled monolayer (SAM) arrays ([similar]0.1–1 GPa), while cell adhesion was tuned by varying the presentation of Arg-Gly-Asp (RGD)-containing peptides. HT-1080 motility was insensitive to cell adhesion ligand density on RGD-SAMs, as they migrated with similar speed and directionality for a wide range of RGD densities (0.2–5% mol fraction RGD). Similarly, HT-1080 migration speed was weakly dependent on adhesion on 0.34 kPa PEG surfaces. On 13 kPa surfaces, a sharp initial increase in cell speed was observed at low RGD concentration, with no further changes observed as RGD concentration was increased further. An increase in cell speed [similar]two-fold for the 13 kPa relative to the 0.34 kPa PEG surface suggested an important role for substrate stiffness in mediating motility, which was confirmed for HT-1080s migrating on variable modulus PEG hydrogels with constant RGD concentration. Notably, despite [similar]two-fold changes in cell speed over a wide range of moduli, HT-1080s adopted rounded morphologies on all surfaces investigated, which contrasted with well spread primary human mesenchymal stem cells (hMSCs). Taken together, our results demonstrate that HT-1080s are morphologically distinct from primary mesenchymal cells (hMSCs) and migrate with minimal dependence on cell adhesion for surfaces within a wide range of moduli, whereas motility is strongly influenced by matrix mechanical properties.
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A Quantitative Comparison of Human HT-1080 Fibrosarcoma Cells and Primary Human Dermal Fibroblasts Identifies a 3D Migration Mechanism with Properties Unique to the Transformed Phenotype
PLoS One
Here, we describe an engineering approach to quantitatively compare migration, morphologies, and adhesion for tumorigenic human fibrosarcoma cells (HT-1080s) and primary human dermal fibroblasts (hDFs) with the aim of identifying distinguishing properties of the transformed phenotype. Relative adhesiveness was quantified using self-assembled monolayer (SAM) arrays and proteolytic 3-dimensional (3D) migration was investigated using matrix metalloproteinase (MMP)-degradable poly(ethylene glycol)…
Here, we describe an engineering approach to quantitatively compare migration, morphologies, and adhesion for tumorigenic human fibrosarcoma cells (HT-1080s) and primary human dermal fibroblasts (hDFs) with the aim of identifying distinguishing properties of the transformed phenotype. Relative adhesiveness was quantified using self-assembled monolayer (SAM) arrays and proteolytic 3-dimensional (3D) migration was investigated using matrix metalloproteinase (MMP)-degradable poly(ethylene glycol) (PEG) hydrogels (“synthetic extracellular matrix” or “synthetic ECM”). In synthetic ECM, hDFs were characterized by vinculin-containing features on the tips of protrusions, multipolar morphologies, and organized actomyosin filaments. In contrast, HT-1080s were characterized by diffuse vinculin expression, pronounced β1-integrin on the tips of protrusions, a cortically-organized F-actin cytoskeleton, and quantitatively more rounded morphologies, decreased adhesiveness, and increased directional motility compared to hDFs. Further, HT-1080s were characterized by contractility-dependent motility, pronounced blebbing, and cortical contraction waves or constriction rings, while quantified 3D motility was similar in matrices with a wide range of biochemical and biophysical properties (including collagen) despite substantial morphological changes. While HT-1080s were distinct from hDFs for each of the 2D and 3D properties investigated, several features were similar to WM239a melanoma cells, including rounded, proteolytic migration modes, cortical F-actin organization, and prominent uropod-like structures enriched with β1-integrin, F-actin, and melanoma cell adhesion molecule (MCAM/CD146/MUC18). Importantly, many of the features observed for HT-1080s were analogous to cellular changes induced by transformation, including cell rounding, a disorganized F-actin cytoskeleton, altered organization of focal adhesion proteins, and a weakly adherent phenotype....
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Measurement of Tissue Adherence for In Situ Formed PEG Acrylate Hydrogels
Presented at the 2010 Society for Biomaterials Annual Meeting, April 21-24 2010, Seattle WA
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We are honored that Mayo Clinic is once again recognized on U.S. News & World Report's Best Hospitals 2024-2025 Honor Roll, with Mayo Clinic in…
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We had a great time meeting our new #RadResidents and #RadFellows at our annual welcome picnic! We're happy to have you in the department, and look…
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