Mustafa Barbhuiya

Gallbladder cancer is an uncommon but lethal malignancy with particularly high incidence in Chile, India, Japan and China. There is a paucity of unbiased large-scale studies investigating molecular basis of gallbladder cancer. To systematically identify differentially regulated proteins in gallbladder cancer, iTRAQ-based quantitative proteomics of gallbladder cancer was carried out using Fourier transform high resolution mass spectrometry. Of the 2575 proteins identified, proteins upregulated… Show more

Gallbladder cancer is an uncommon but lethal malignancy with particularly high incidence in Chile, India, Japan and China. There is a paucity of unbiased large-scale studies investigating molecular basis of gallbladder cancer. To systematically identify differentially regulated proteins in gallbladder cancer, iTRAQ-based quantitative proteomics of gallbladder cancer was carried out using Fourier transform high resolution mass spectrometry. Of the 2575 proteins identified, proteins upregulated in gallbladder cancer included several lysosomal proteins such as prosaposin, cathepsin Z and cathepsin H. Downregulated proteins included serine protease HTRA1 and transgelin, which have been reported to be downregulated in several other cancers. Novel biomarker candidates including prosaposin and transgelin were validated to be upregulated and downregulated, respectively, in gallbladder cancer using tissue microarrays. Our study provides the first large scale proteomic characterization of gallbladder cancer which will serve as a resource for future discovery of biomarkers for gallbladder cancer. Show less

There is marked increase in the incidence of gall bladder cancer (GBC) in Indian subcontinent in recent times. No hospital based data or registry is available for this important cancer from North Central province of India. The present study aims to make a first insight into the pattern of GBC in North Central region of India during the year (2007-08). A retrospective study was carried out at the Cancer Hospital and Research Institute, Gwalior, Madhya Pradesh, India, to identify the pattern of… Show more

There is marked increase in the incidence of gall bladder cancer (GBC) in Indian subcontinent in recent times. No hospital based data or registry is available for this important cancer from North Central province of India. The present study aims to make a first insight into the pattern of GBC in North Central region of India during the year (2007-08). A retrospective study was carried out at the Cancer Hospital and Research Institute, Gwalior, Madhya Pradesh, India, to identify the pattern of GBC in different districts of north central India. The data obtained were analyzed for statistical significance by calculating the average value of the parameters followed by sample t-test using Graph Pad Prism 5. Most of the patients were from rural background with poor economic status (55.07%) with low body mass index (BMI =18.5) and were either uneducated or educated up to primary level only. The crude incidence rate of GBC was 15.5/1, 00,000 in females and 5.9/1, 00,000 in males. The female/male ratio was 2.3 for gall bladder diseases. Show less

This is my PhD project aims to discover novel biomarkers for gallbladder cancer by quantitative proteomic profiling of gallbladder tumor, adjacent non tumor and calculus cholecystitis tissue. In addition, quantitative proteomic profiling of bile juice from gallbladder of cancer and calculus cholecystitis patients. A baseline bile proteome from three calculus cholecystits subject is made available to public through the efforts as part of the project!