“Neil is a detailed-oriented early riser who enjoys taking charge of difficult tasks and seeing them through in a timely and orderly manner. Any research group dedicated to analysis and evaluation of polymers and medical devices would be fortunate to have Neil amongst their ranks.”
Neil Anderson, PhD
Boulder, Colorado, United States
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Given RVNU introduced itself to the world last week I thought I'd take the time (re)intro myself to you all too. When all is said and done, I'm a…
Given RVNU introduced itself to the world last week I thought I'd take the time (re)intro myself to you all too. When all is said and done, I'm a…
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This week at Eric, we're starting a series focused on loneliness. Recent conversations highlighted that post-Covid, loneliness seems to have worsened…
This week at Eric, we're starting a series focused on loneliness. Recent conversations highlighted that post-Covid, loneliness seems to have worsened…
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There are many great career opportunities at Front Range Community College 👇🏻👇🏻👇🏻 To learn more, reach out to Dr. Chris McGilvray.
There are many great career opportunities at Front Range Community College 👇🏻👇🏻👇🏻 To learn more, reach out to Dr. Chris McGilvray.
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Ion-beam sputtered (IBS) thin-film interference filters for nonlinear optical imaging
Proceedings of the SPIE
Nonlinear optical (NLO) microscopy is emerging as a powerful technique for the study of biological samples. By combining several different imaging modalities such as multiphoton (MP) fluorescence, second-harmonic and thirdharmonic generation (SHG and THG), and coherent Raman scattering techniques such as coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS), it is possible to combine the best practices of label and label-free imaging into a single platform capable…
Nonlinear optical (NLO) microscopy is emerging as a powerful technique for the study of biological samples. By combining several different imaging modalities such as multiphoton (MP) fluorescence, second-harmonic and thirdharmonic generation (SHG and THG), and coherent Raman scattering techniques such as coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS), it is possible to combine the best practices of label and label-free imaging into a single platform capable of imaging structures within single cells and elucidating the health of biological tissue samples, even at the submicron level. Single-substrate, ion-beam-sputtered (IBS) thinfilm interference filters are a key enabling technology in laser-based optical microscopy and play a critical role in multimodal NLO imaging. In microscopy applications, optical filters are used to select and discriminate exactly which wavelengths of light are to be transmitted, reflected and suppressed. In this paper we discuss various important characteristics of hard-coated thin-film interference filters, such as high light throughput, steep edges, and high out-of-band blocking, all of which require careful consideration when designing and manufacturing optical filters for NLO imaging applications. To understand the true performance of hard-coated IBS filters, a simple CARS imaging experiment was performed. We found a 2.6 times increase in signal enhancement and 70% improvement in image contrast when compared to a commercially available filter commonly used in CARS microscopy applications.
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