“I worked with Pranjali in KUMC for almost a year and half and she has a great role in mentoring my early postdoc skills as senior colleague. Pranjali besides having strong knowledge of subject she has a great management skill and Team work quality. Working with her was always a pleasant experience and I learned many scientific skills from her very conveniently due to her excellent communication skill during my training period in lab. Her strong dedication towards work can be motivation for any of her colleague. Personally she is very helpful and nice with her colleague, she was a key factor to maintain our lab atmosphere lively. I wish her lots of success in life. ”
Pranjali Dalvi
Dublin, California, United States
572 followers
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Activity
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ASF hosted its first-ever Bring Your Brood to Work Day where over 250 guardians and little people explored our labs, demonstration rooms, and work…
ASF hosted its first-ever Bring Your Brood to Work Day where over 250 guardians and little people explored our labs, demonstration rooms, and work…
Liked by Pranjali Dalvi
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Excited to share our latest article with #iPVDc team published in @plospathogens: https://lnkd.in/gfH5C2eF #plospathogensauthor #PVRI #longcovid…
Excited to share our latest article with #iPVDc team published in @plospathogens: https://lnkd.in/gfH5C2eF #plospathogensauthor #PVRI #longcovid…
Liked by Pranjali Dalvi
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Journal Reviewer
PLOS ONE, Respiratory Research, Journal of Pharmacy and Pharmacology, EC Microbiology
- Present 11 years 7 months
Science and Technology
Publications
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TNB-738, a biparatopic antibody, boosts intracellular NAD+ by inhibiting CD38 ecto-enzyme activity
MAbs, Taylor & Francis
Cluster of differentiation 38 (CD38) is an ecto-enzyme expressed primarily on immune cells that metabolizes nicotinamide adenine dinucleotide (NAD+) to adenosine diphosphate ribose or cyclic ADP-ribose and nicotinamide. Other substrates of CD38 include nicotinamide adenine dinucleotide phosphate and nicotinamide mononucleotide, a critical NAD+ precursor in the salvage pathway. NAD+ is an important coenzyme involved in several metabolic pathways and is a required cofactor for the function of…
Cluster of differentiation 38 (CD38) is an ecto-enzyme expressed primarily on immune cells that metabolizes nicotinamide adenine dinucleotide (NAD+) to adenosine diphosphate ribose or cyclic ADP-ribose and nicotinamide. Other substrates of CD38 include nicotinamide adenine dinucleotide phosphate and nicotinamide mononucleotide, a critical NAD+ precursor in the salvage pathway. NAD+ is an important coenzyme involved in several metabolic pathways and is a required cofactor for the function of sirtuins (SIRTs) and poly (adenosine diphosphate-ribose) polymerases. Declines in NAD+ levels are associated with metabolic and inflammatory diseases, aging, and neurodegenerative disorders. To inhibit CD38 enzyme activity and boost NAD+ levels, we developed TNB-738, an anti-CD38 biparatopic antibody that pairs two non-competing heavy chain-only antibodies in a bispecific format. By simultaneously binding two distinct epitopes on CD38, TNB-738 potently inhibited its enzymatic activity, which in turn boosted intracellular NAD+ levels and SIRT activities. Due to its silenced IgG4 Fc, TNB-738 did not deplete CD38-expressing cells, in contrast to the clinically available anti-CD38 antibodies, daratumumab, and isatuximab. TNB-738 offers numerous advantages compared to other NAD-boosting therapeutics, including small molecules, and supplements, due to its long half-life, specificity, safety profile, and activity. Overall, TNB-738 represents a novel treatment with broad therapeutic potential for metabolic and inflammatory diseases associated with NAD+ deficiencies.
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Attenuating CD3 affinity in a PSMAxCD3 bispecific antibody enables killing of prostate tumor cells with reduced cytokine release
Journal for Immunotherapy of Cancer
Therapeutic options currently available for metastatic castration-resistant prostate cancer (mCRPC) do not extend median overall survival >6 months. Therefore, the development of novel and effective therapies for mCRPC represents an urgent medical need. T cell engagers (TCEs) have emerged as a promising approach for the treatment of mCRPC due to their targeted mechanism of action. However, challenges remain in the clinic due to the limited efficacy of TCEs observed thus far in solid tumors…
Therapeutic options currently available for metastatic castration-resistant prostate cancer (mCRPC) do not extend median overall survival >6 months. Therefore, the development of novel and effective therapies for mCRPC represents an urgent medical need. T cell engagers (TCEs) have emerged as a promising approach for the treatment of mCRPC due to their targeted mechanism of action. However, challenges remain in the clinic due to the limited efficacy of TCEs observed thus far in solid tumors as well as the toxicities associated with cytokine release syndrome (CRS) due to the usage of high-affinity anti-CD3 moieties such as OKT3. Using genetically engineered transgenic rats (UniRat and OmniFlic) that express fully human IgG antibodies together with an NGS-based antibody discovery pipeline, we developed TNB-585, an anti-CD3xPSMA TCE for the treatment of mCRPC. TNB-585 pairs a tumor-targeting anti-PSMA arm together with a unique, low-affinity anti-CD3 arm in bispecific format. In vitro, TNB-585 induced activation and proliferation of human T cells resulting in the killing of PSMA+ prostate tumor cells in both 2D cultures and 3D spheroids with minimal cytokine release and reduced regulatory T cell activation compared with a positive control antibody that contains the same anti-PSMA arm but a higher affinity anti-CD3 arm (comparable with OKT3). In addition, TNB-585 demonstrated potent efficacy against patient-derived prostate tumors ex vivo and induced immune cell infiltration and dose-dependent tumor regression in vivo in established C4-2 tumor. Hence, TNB-585, with its low-affinity anti-CD3, may be efficacious while inducing a lower incidence and severity of CRS in patients with prostate cancer compared with TCEs that incorporate high-affinity anti-CD3 domains.
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Immune activated monocyte exosomes alter microRNAs in brain endothelial cells and initiate an inflammatory response through the TLR4/MyD88 pathway
Scientific Reports
The host immune response is critical for homeostasis; however, when chronic low level activation of the immune response with or without the driver continues, a cascade of events can trigger immunological dysfunction. Monocytes are key peripheral sensors of the immune response and their activation is instrumental in the development of cognitive impairment. Here, we show that monocytes activated by interferon alpha, lipopolysaccharide or a combination of both generate exosomes carrying…
The host immune response is critical for homeostasis; however, when chronic low level activation of the immune response with or without the driver continues, a cascade of events can trigger immunological dysfunction. Monocytes are key peripheral sensors of the immune response and their activation is instrumental in the development of cognitive impairment. Here, we show that monocytes activated by interferon alpha, lipopolysaccharide or a combination of both generate exosomes carrying significantly altered microRNA profiles compared to non-activated monocytes. These exosomes alone can activate human brain microvascular endothelial cells to stimulate adhesion molecules, CCL2, ICAM1, VCAM1 and cytokines, IL1β and IL6. This activation is through the toll like receptor 4 (TLR4)/myeloid differentiation primary response gene 88 (MyD88) pathway that activates nuclear factor-κB and increases monocyte chemotaxis. Inhibition of monocyte exosome release reverses endothelial cell activation and monocyte chemotaxis. Our study suggests that activated monocytes have an impact on brain vascular function through intercellular exosome signaling.
Other authorsSee publication -
Role of autophagy in exacerbated proliferation of morphine and HIV-Tat exposed human pulmonary endothelial cells and SIV-infected macaques exposed to morphine
Autophagy
Intravenous drug use is one of the major risk factors for HIV-infection in HIV-related pulmonary arterial hypertension patients. We previously demonstrated exaggerated pulmonary vascular remodeling with enhanced apoptosis followed by increased proliferation of pulmonary endothelial cells on simultaneous exposure to both opioids and HIV protein(s). Here we hypothesize that the exacerbation of autophagy may be involved in the switching of endothelial cells from an early apoptotic state to later…
Intravenous drug use is one of the major risk factors for HIV-infection in HIV-related pulmonary arterial hypertension patients. We previously demonstrated exaggerated pulmonary vascular remodeling with enhanced apoptosis followed by increased proliferation of pulmonary endothelial cells on simultaneous exposure to both opioids and HIV protein(s). Here we hypothesize that the exacerbation of autophagy may be involved in the switching of endothelial cells from an early apoptotic state to later hyper-proliferative state. Treatment of human pulmonary microvascular endothelial cells (HPMECs) with both the HIV-protein Tat and morphine resulted in an oxidative stress-dependent increase in the expression of various markers of autophagy and formation of autophagosomes when compared to either Tat or morphine monotreatments as demonstrated by western blot, transmission electron microscopy and immunofluorescence. Autophagy flux experiments suggested increased formation rather than decreased clearance of autolysosomes. Inhibition of autophagy resulted in a significant increase in apoptosis and reduction in proliferation of HPMECs with combined morphine and Tat (M+T) treatment compared to monotreatments whereas stimulation of autophagy resulted in opposite effects. Significant increases in the expression of autophagy markers as well as the number of autophagosomes and autolysosomes was observed in the lungs of SIV-infected macaques and HIV-infected humans exposed to opioids. Overall our findings indicate that morphine in combination with viral protein(s) results in the induction of autophagy in pulmonary endothelial cells that may lead to an increase in severity of angio-proliferative remodeling of the pulmonary vasculature on simian and human immunodeficiency virus infection in the presence of opioids.
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Involvement Of HIV Infected And Cocaine Exposed Macrophage Derived Extracellular Vesicles In Pulmonary Smooth Muscle Hyperplasia
American Journal of Respiratory and Critical Care Medicine
We previously demonstrated enhanced pulmonary vascular remodeling in arteries of HIV-1 infected IVDU exposed to opioids and/or cocaine compared with HIV-infected non-drug abusers or un-infected IVDU. Furthermore, inflammation is known to play a crucial role in vascular remodeling associated with HIV related chronic lung diseases.
An average of 40-250 nm sized, cup-shaped EV were observed by TEM analysis. The protein extracts from EV and whole cell lysate analyzed for exosomal markers…We previously demonstrated enhanced pulmonary vascular remodeling in arteries of HIV-1 infected IVDU exposed to opioids and/or cocaine compared with HIV-infected non-drug abusers or un-infected IVDU. Furthermore, inflammation is known to play a crucial role in vascular remodeling associated with HIV related chronic lung diseases.
An average of 40-250 nm sized, cup-shaped EV were observed by TEM analysis. The protein extracts from EV and whole cell lysate analyzed for exosomal markers Tsg-101, Alix and CD9 suggested extracellular vesicle (EV) preparation to be highly enriched in exosomes. Nanosight quantification revealed higher concentration of total nanoparticles in the EV-preparation from HIV+cocaine-MDM compared to EV-preparation from HIV- or cocaine-MDM, with significant increase being in the particle size range of 51-100nm. We also found significantly higher ratio of EV-protein to cell lysate protein in HIV+cocaine- MDM when compared with other groups. Treatment of HPASMC with HIV+cocaine-MDM derived supernatant resulted in significant increase in the cell proliferation compared to supernatants from mono-treated or untreated cells. However, no increase in the proliferation of HPASMC was observed when exposed to supernatant from HIV+cocaine-MDM pretreated with exosome release inhibitor:GW4869. Uptake of fluorescent labeled EV by HPASMC was clearly observed within 2 hours of exposure. Consequently, direct exposure of HPASMC to EV from HIV+/-cocaine MDM also resulted in significant increase in the proliferation of cells compared to EV released from control MDM.
Thus, HIV-infection and cocaine mediated alterations in the macrophage-derived EV promote hyper-proliferation of smooth muscle cells through delivery of its molecular cargo.
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Effect of Cocaine on Pulmonary Vascular Remodeling and Hemodynamics in HIV-transgenic rats
American Journal of Respiratory Cell and Molecular Biology
Human-immunodeficiency related pulmonary arterial hypertension has been found to be more prevalent in intravenous drug users (IVDUs). Our earlier cell-culture findings reported down-regulation of bone morphogenetic protein receptors (BMPRs) in combination with enhanced proliferation of human pulmonary arterial smooth muscle cells (PASMCs) in the presence of HIV-Tat and cocaine compared to either treatment alone. Here, we report physiologic evidence of significant increases in mean pulmonary…
Human-immunodeficiency related pulmonary arterial hypertension has been found to be more prevalent in intravenous drug users (IVDUs). Our earlier cell-culture findings reported down-regulation of bone morphogenetic protein receptors (BMPRs) in combination with enhanced proliferation of human pulmonary arterial smooth muscle cells (PASMCs) in the presence of HIV-Tat and cocaine compared to either treatment alone. Here, we report physiologic evidence of significant increases in mean pulmonary arterial pressure (mPAP) in HIV-transgenic (HIV-Tg) rats intraperitoneally administered with 40 mg/kg body wt. cocaine (HIV-cocaine group) once daily for 21 days when compared with HIV-Tg rats given saline (HIV group) or Wild type Fischer 334 rats treated with (WT-cocaine group) and without cocaine (WT group). In addition, right ventricle systolic pressure (RVSP) was also found significantly higher in HIV-cocaine rats compared to WT group. Significant down-regulation in protein expression of BMPR-2 and BMPR-1B was observed in total lung extract from HIV-cocaine rats, compared to other three groups. Furthermore, the PASMCs isolated from HIV-cocaine rats demonstrated higher level of proliferation and lower levels of apoptosis compared to cells isolated from other rat groups. Interestingly, in corroboration of our earlier cell-culture findings, we observed higher expression of BMPR-2 and -1B mRNA and significant lower levels of BMPR-2 and BMPR-1B protein in HIV-cocaine PASMCs compared to cells isolated from all other groups. In conclusion, our findings support an additive effect of cocaine and HIV on smooth muscle dysfunction resulting in enhanced pulmonary vascular remodeling with associated elevation of mPAP and RVSP in HIV-Tg rats exposed to cocaine.
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Ligand Independent Activation Of PDGFRβ During HIV-Tat And Cocaine Mediated Smooth Muscle Hyperplasia
American Journal of Respiratory Cell and Molecular Biology
Our previous study supports an additive effect of cocaine to human immunodeficiency virus infection in the development of pulmonary arteriopathy through enhancement of proliferation of pulmonary smooth muscle cells (SMCs), while also suggesting involvement of platelet-derived growth factor receptor (PDGFR) activation in the absence of further increase in PDGF-BB ligand. Redox-related signaling pathways have been shown to regulate tyrosine kinase receptors independent of ligand binding, so we…
Our previous study supports an additive effect of cocaine to human immunodeficiency virus infection in the development of pulmonary arteriopathy through enhancement of proliferation of pulmonary smooth muscle cells (SMCs), while also suggesting involvement of platelet-derived growth factor receptor (PDGFR) activation in the absence of further increase in PDGF-BB ligand. Redox-related signaling pathways have been shown to regulate tyrosine kinase receptors independent of ligand binding, so we hypothesized that simultaneous treatment of SMCs with transactivator of transcription (Tat) and cocaine may be able to indirectly activate PDGFR through modulation of reactive oxygen species (ROS) without the need for PDGF binding. We found that blocking the binding of ligand using suramin or monoclonal IMC-3G3 antibody significantly reduced ligand-induced autophosphorylation of Y1009 without affecting ligand-independent transphosphorylation of Y934 residue on PDGFRβ in human pulmonary arterial SMCs treated with both cocaine and Tat. Combined treatment of human pulmonary arterial SMCs with cocaine and Tat resulted in augmented production of superoxide radicals and hydrogen peroxide when compared with either treatment alone. Inhibition of this ROS generation prevented cocaine- and Tat-mediated Src activation and transphosphorylation of PDGFRβ at Y934 without any changes in phosphorylation of Y1009, in addition to attenuation of smooth muscle hyperplasia. Furthermore, pretreatment with an Src inhibitor, PP2, also suppressed cocaine- and Tat-mediated enhanced Y934 phosphorylation and smooth muscle proliferation. Finally, we report total abrogation of cocaine- and Tat-mediated synergistic increase in cell proliferation on inhibition of both ligand-dependent and ROS/Src-mediated ligand-independent phosphorylation of PDGFRβ.
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Augmentation Of Tgf-β Signaling In Hiv-Tat And Cocaine Treated Pulmonary Smooth Muscle Cells: Implications For HIV-PAH
American Journal of Respiratory and Critical Care Medicine
Rationale: We earlier demonstrated enhanced pulmonary vascular remodeling and severe down-modulation of bone morphogenetic protein receptor (BMPR), the anti-proliferative arm of TGF-β signaling, in lungs of HIV+IVDUs compared to HIV / IVDU or normal lungs. Further, using in-vitro cell culture system, we showed exacerbated down-modulation of BMPR axis during cocaine and HIV-Tat mediated smooth muscle hyperplasia (Arterioscler Thromb Vasc Biol. 2013 Nov; 33(11):2585-95). Here, we now delineate…
Rationale: We earlier demonstrated enhanced pulmonary vascular remodeling and severe down-modulation of bone morphogenetic protein receptor (BMPR), the anti-proliferative arm of TGF-β signaling, in lungs of HIV+IVDUs compared to HIV / IVDU or normal lungs. Further, using in-vitro cell culture system, we showed exacerbated down-modulation of BMPR axis during cocaine and HIV-Tat mediated smooth muscle hyperplasia (Arterioscler Thromb Vasc Biol. 2013 Nov; 33(11):2585-95). Here, we now delineate the effect of HIV-Tat and cocaine on the proliferative arm of TGF-β signaling.
Results: A significant increase in the levels of TGF-β was observed in cocaine-Tat treated HPASMC supernatants compared to Tat or cocaine treatment alone. Enhanced expression of TGFβR1, TGFβR2 and p-SMAD2/3 along with significant increase in the mRNA of SMAD dependent downstream target genes: PAI-1 and IL-6 was observed on combined treatment with cocaine and Tat compared to mono-treatments. Interestingly, a distinct increase in TGFβR1-TAK1 complex and reduction in BMPR2-TAK1 complex was observed in cocaine-Tat treated compared to untreated cells. In support to this finding a significant increase in the expression of TAK1 mediated SMAD independent downstream signaling molecules: p-MKK4 and p-JNK was observed with cocaine-Tat treatment compared to either treatment alone. Additionally, inhibition of TGF-β signaling prevented the cocaine and Tat mediated SMC hyperplasia.
Conclusion: Both activation of proliferative TGF-β signaling and down-modulation of anti-proliferative BMPR signaling are involved in cocaine and HIV-protein mediated smooth muscle dysfunction.
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HIV-1/ cocaine Induced Oxidative Stress Disrupts Tight Junction Protein-1 in Human Pulmonary Microvascular Endothelial Cells: Role of Ras/ERK1/2 Pathway
PLOS ONE
Intravenous drug use (IVDU) is the major risk factor in the development of HIV-related pulmonary arterial hypertension (HRPAH); however, the pathogenesis of HRPAH in association with IVDU has yet to be characterized. Endothelial injury is considered to be an initiating factor for pulmonary vascular remodeling in animal models of PAH. Our previous study shows that simultaneous exposure to HIV-Trans-activator of transcription (Tat) and cocaine exacerbates both disruption of tight junction…
Intravenous drug use (IVDU) is the major risk factor in the development of HIV-related pulmonary arterial hypertension (HRPAH); however, the pathogenesis of HRPAH in association with IVDU has yet to be characterized. Endothelial injury is considered to be an initiating factor for pulmonary vascular remodeling in animal models of PAH. Our previous study shows that simultaneous exposure to HIV-Trans-activator of transcription (Tat) and cocaine exacerbates both disruption of tight junction proteins and permeability of human pulmonary artery endothelial cells compared with either treatment alone. We here now demonstrate that this HIV-Tat and cocaine mediated endothelial dysfunction accompanies with increase in hydrogen peroxide and superoxide radicals generation and involves redox sensitive signaling pathway. Pretreatment with antioxidant cocktail attenuated the cocaine and Tat mediated disassembly of Zonula Occludens (ZO)-1 and enhancement of endothelial monolayer permeability. Furthermore, inhibition of NADPH oxidase by apocynin or siRNA-mediated knockdown of gp-91(phox) abolished the Tat/cocaine-induced reactive oxygen species (ROS) production, suggesting the NADPH oxidase mediated generation of oxidative radicals. In addition, ROS dependent activation of Ras and ERK1/2 Kinase was observed to be mediating the TJP-1 disassembly, and endothelial dysfunction in response to cocaine and Tat exposure. In conclusion, our findings demonstrate that Tat/cocaine -mediated production of ROS activate Ras/Raf/ERK1/2 pathway that contributes to disruption of tight junction protein leading to pulmonary endothelial dysfunction associated with pulmonary vascular remodeling.
Other authors -
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Down-regulation of BMPR Axis during HIV and Cocaine-mediated Pulmonary Smooth Muscle Hyperplasia: Implications for HRPAH
Arteriosclerosis, Thrombosis and Vascular Biology
OBJECTIVE:
Our previous findings support an additive effect of cocaine to HIV infection in the development of pulmonary arteriopathy through enhanced proliferation of human pulmonary smooth muscle cells. We now examined the role of antiproliferative bone morphogenetic protein receptor (BMPR) axis in HIV protein and cocaine-mediated pulmonary smooth muscle hyperplasia.
APPROACH AND RESULTS:
Stimulation of BMPR axis resulted in attenuation of synergistic increase in the proliferation of…OBJECTIVE:
Our previous findings support an additive effect of cocaine to HIV infection in the development of pulmonary arteriopathy through enhanced proliferation of human pulmonary smooth muscle cells. We now examined the role of antiproliferative bone morphogenetic protein receptor (BMPR) axis in HIV protein and cocaine-mediated pulmonary smooth muscle hyperplasia.
APPROACH AND RESULTS:
Stimulation of BMPR axis resulted in attenuation of synergistic increase in the proliferation of human pulmonary arterial smooth muscle cells in response to cocaine and HIV protein, transactivator of transcription (Tat). Interestingly, an increase in mRNA but decrease in protein levels of BMPR with correlated decrease in the activation of Sma- and MAD-related family protein 1/5/8 and Id1 gene expression was observed on combined treatment with cocaine and Tat when compared with the untreated cells at all time points tested. Although longer exposure to either cocaine or Tat alone also resulted in a significant decrease in the BMPR protein expression, the abrogation on combined treatment was still significantly more when compared with that of the monotreatments. Significant increase in mRNA but downmodulation of BMPR protein expression was also observed in the lung extracts from HIV-infected intravenous drug users (HIV+IVDU) when compared with that from HIV-infected non-IVDUs (HIV) or uninfected IVDUs (IVDU). Furthermore, significant decrease in BMPR protein expression was also observed in HIV or IVDUs when compared with normal controls that correlated with in vitro findings on chronic exposure to cocaine or HIV protein alone.
CONCLUSIONS:
Simultaneous exposure of pulmonary smooth muscle cells to viral protein(s) and cocaine exacerbates downregulation of BMPR axis that may result in enhanced pulmonary vasculature aberrations in HIV+IVDUs.Other authors -
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Functional characterization and molecular expression of large neutral amino acid transporter (LAT1) in human prostate cancer cells.
International Journal of Pharmacy
The primary objective of this study is to functionally characterize and provide molecular evidence of large neutral amino acid transporter (LAT1) in human derived prostate cancer cells (PC-3). We carried out the uptake of [3H]-tyrosine to assess the functional activity of LAT1. Reverse transcription-polymerase chain reaction (RT-PCR) analysis is carried out to confirm the molecular expression of LAT1. [3H]-tyrosine uptake is found to be time dependent and linear up to 60 min. The uptake process…
The primary objective of this study is to functionally characterize and provide molecular evidence of large neutral amino acid transporter (LAT1) in human derived prostate cancer cells (PC-3). We carried out the uptake of [3H]-tyrosine to assess the functional activity of LAT1. Reverse transcription-polymerase chain reaction (RT-PCR) analysis is carried out to confirm the molecular expression of LAT1. [3H]-tyrosine uptake is found to be time dependent and linear up to 60 min. The uptake process does not exhibit any dependence on sodium ions, pH and energy. However, it is temperature dependent and found maximal at physiological temperature. The uptake of [3H]-tyrosine demonstrates saturable kinetics with K(m) and V(max) values of 34 ± 3 μM and 0.70 ± 0.02 nanomoles/min/mg protein, respectively. It is strongly inhibited by large neutral (phenylalanine, tryptophan, leucine, isoleucine) and small neutral (alanine, serine, cysteine) but not by basic (lysine and arginine) and acidic (aspartic and glutamic acid) amino acids. Isoleucine-quinidine (Ile-quinidine) prodrug generates a significant inhibitory effect on [3H]-tyrosine uptake suggesting that it is recognized by LAT1. RT-PCR analysis provided a product band at 658 and 840 bp, specific to LAT1 and LAT2, respectively. For the first time, this study demonstrates that LAT1, primarily responsible for the uptake of large neutral amino acids, is functionally active in PC-3 cells. Significant increase in the uptake generated by Ile-quinidine relative to quinidine suggests that LAT1 can be utilized for enhancing the cellular permeation of poor cell permeable anticancer drugs. Furthermore, this cell line can be utilized as an excellent in vitro model for studying the interaction of large neutral amino acid conjugated drugs with LAT1 transporter.
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Enhanced Pulmonary Arteriopathy in SIV-Infected Macaques Exposed to Morphine.
American Journal of Respiratory and Critical Care Medicine
RATIONALE:
HIV-associated pulmonary arterial hypertension (PAH) is likely a more prevalent noninfectious complication of AIDS than previously recognized. Furthermore, the majority of HIV-PAH cases occur in individuals with a history of intravenous drug use. In this study we used a simian immunodeficiency (SIV) macaque model and a primary cell-culture system to investigate the association between drug abuse and HIV infection in HIV-PAH development.
METHODS:
The archival lung tissues…RATIONALE:
HIV-associated pulmonary arterial hypertension (PAH) is likely a more prevalent noninfectious complication of AIDS than previously recognized. Furthermore, the majority of HIV-PAH cases occur in individuals with a history of intravenous drug use. In this study we used a simian immunodeficiency (SIV) macaque model and a primary cell-culture system to investigate the association between drug abuse and HIV infection in HIV-PAH development.
METHODS:
The archival lung tissues from macaques previously used to study the effect of morphine on SIV infection-associated neuropathogenesis were analyzed for pulmonary vascular changes. The direct effect of HIV proteins and illicit drugs was investigated on oxidative stress, survival, and proliferation of human pulmonary microvascular endothelial cells.
MEASUREMENTS AND MAIN RESULTS:
SIVmacR71/17E-infected rhesus macaques treated with morphine (VM group) demonstrated significant pulmonary vascular remodeling, including the presence of early and advanced complex (plexiform) lesions, when compared with either the SIV-infected (V group) or morphine-treated uninfected (M group) macaques. However, both the V (two of five) and VM (two of six) groups included some animals with Pneumocystis jirovecii pneumonia. The endothelial cells lining the vessels with medial hypertrophy or initial-stage intimal lesions in lung sections from VM macaques demonstrated an increase in positivity for both terminal dUTP nick-end labeling and Ki67. Oxidative stress-mediated enhanced apoptosis followed by enhanced proliferation of endothelial cells was observed on simultaneous treatment with viral proteins and drugs of abuse compared with either treatment alone.
CONCLUSIONS:
Our findings suggest that SIV/HIV protein(s) and morphine interact to cause the proliferation of apoptosis-resistant endothelial cells leading to angio-obliteration.Other authors -
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Emergence of Fluoroquinolone Resistance on Intrinsic Expression of P-glycoprotein Phenotype in Corneal Epithelial Cells
Journal of Ocular Pharmacology and Therapeutics
PURPOSE:
Multidrug resistance (MDR) represents a major obstacle to the success of antimicrobial fluoroquinolone (FQ) therapy. MDR-associated efflux protein pumps antimicrobial agents out of the corneal cells, leading to suboptimal eradication of microbes. This article examines whether long-term FQ (levofloxacin, ofloxacin, and gatifloxacin) therapy can modify the MDR phenotype (P-glycoprotein [P-gp]) on corneal epithelial cells (Statens Seruminstitut Rabbit Cornea [SIRC]).
METHODS:
To…PURPOSE:
Multidrug resistance (MDR) represents a major obstacle to the success of antimicrobial fluoroquinolone (FQ) therapy. MDR-associated efflux protein pumps antimicrobial agents out of the corneal cells, leading to suboptimal eradication of microbes. This article examines whether long-term FQ (levofloxacin, ofloxacin, and gatifloxacin) therapy can modify the MDR phenotype (P-glycoprotein [P-gp]) on corneal epithelial cells (Statens Seruminstitut Rabbit Cornea [SIRC]).
METHODS:
To study the effect of FQ, SIRC cells without any exposure to FQ (control) were compared with the cells exposed to ofloxacin, levofloxacin, and gatifloxacin at a concentration of 10 μg/mL for 3 weeks. Efflux activity of P-gp was assessed by in vitro uptake studies (fluorescent and radioactive), flow cytometry, and quantitative real-time polymerase chain reaction (qRT-PCR).
RESULTS:
In the presence of FQ, elevated P-gp expression was noted with uptake, flow cytometry, and qRT-PCR analyses. This study confirms that long-term exposure to antibiotics, particularly FQ, can induce overexpression of P-gp efflux transporter present on the corneal cells. P-gp overexpression is commonly noticed in anticancer drug resistance cell lines; however, for the first time, this report describes overexpression of P-gp due to FQ exposure.
CONCLUSIONS:
Based on this result, it is suggested that strategies should be developed and implemented not only to overcome resistance to ocular pathogen but also to FQ-induced cellular resistance.Other authors -
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Amylase and pectinase from single source for combined desizing and scouring
Indian Journal of Fibre & Textile Research
A wild type bacterial strain isolated from soil rhizosphere has been used to produce amylase and pectinase in a common production media. Single step combined enzymatic desizing and scouring of grey cotton fabric using these enzymes have been done without further purification. The formulated media containing both the enzymes gives higher weight loss and absorbency compared to the commercial enzymatic desizing. The absorption coefficient after treatment with formulated media is found to be…
A wild type bacterial strain isolated from soil rhizosphere has been used to produce amylase and pectinase in a common production media. Single step combined enzymatic desizing and scouring of grey cotton fabric using these enzymes have been done without further purification. The formulated media containing both the enzymes gives higher weight loss and absorbency compared to the commercial enzymatic desizing. The absorption coefficient after treatment with formulated media is found to be similar to that observed after the conventional desized-scoured fabric treatment. It is observed that the study would improve textile processing in terms of ecofriendliness coupled with conservation of time, energy and chemicals.
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Honors & Awards
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Special Early Career Investigator Travel Award
Society of NeuroImmune Pharmacology
Travel award for presenting poster at the 21st Society of NeuroImmune Pharmacology conference, Miami, 22nd to 26th April 2015.
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Early Career Investigator Travel Award
Society of NeuroImmune Pharmacology
Travel Award for presenting research at the 20th Society of NeuroImmune Pharmacology conference, New Orleans, 26th to 29th March 2014.
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Biomedical Research Training Program Award
University of Kansas Medical Center
Fellowship grant for conducting research on "Enhanced Autophagy Of Pulmonary Endothelial Cells On Exposure To HIV-Tat And Morphine: Role In HRPAH Associated Pulmonary Vascular Remodeling"
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Sponsorship award
Rossari Biotech India Pvt. Ltd.
For conducting molecular biology research on developing DNA library of B. subtilis genome for amylase and pectinase enzyme producing genes, developing enzyme over-expressing bacterial clones for developing eco-friendly combined desizing and bioscouring on raw cotton fabric.
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Travel award from Bhavan’s College
Bhavan's College, Mumbai University
Award for traveling from Mumbai, India to Anaheim, CA, USA, conference registration and stay expenses for presenting poster at the American Biomedical Research Conference for Minority Students, Anaheim, California November 2006.
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Fellowship from Rossari Biotech
Rossari Biotech India Pvt. Ltd.
For conducting MS research on "Development of Microbial Consortium for Textile Bioprocessing"
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1st prize in “Scientific Poster Competition on HIV and Molecular Biology”
Bhavan's College, Mumbai University
This was in 3rd year BS
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1st prize for “Best Report writing and Presentation"
Bhavan's College, Mumbai University
This was for report writing and presentation on "Industrial visit to SGS-India Pvt. Ltd.” during 2nd year BS.
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National award for best poster in the field of Microbiology at the 45th Association of Microbiologists of India (AMI) Conference
Association of Microbiologists of India (AMI)
"Overall best poster in the field of Microbiology" for research entitled “Development of a Microbial Consortium for Textile Bioprocessing”, Nov 2004 during 1st year MS.
Languages
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English
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Marathi
Full professional proficiency
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Hindi
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Gujarati
Limited working proficiency
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Sigma Xi
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I'd like to give a shout-out to some ex-Amgen folks who made enormous contributions to the early days of Lumakras, and have since moved on to senior…
I'd like to give a shout-out to some ex-Amgen folks who made enormous contributions to the early days of Lumakras, and have since moved on to senior…
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Thanks so much Ray. What a wonderful honor for Amgen and for your esteemed team! I was very fortunate to be a long- term resident of the KRAS…
Thanks so much Ray. What a wonderful honor for Amgen and for your esteemed team! I was very fortunate to be a long- term resident of the KRAS…
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!!!!! I couldn’t be more proud of my awesome team of champions! Amgen was awarded Prix Galien tonight for Best Pharmaceutical Agent for our KRAS-G12C…
!!!!! I couldn’t be more proud of my awesome team of champions! Amgen was awarded Prix Galien tonight for Best Pharmaceutical Agent for our KRAS-G12C…
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https://lnkd.in/gf7APg3Q
https://lnkd.in/gf7APg3Q
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In principle, all biomolecules have a framework of linked carbon atoms. Life has evolved methods for creating these, but it has proven notoriously…
In principle, all biomolecules have a framework of linked carbon atoms. Life has evolved methods for creating these, but it has proven notoriously…
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"Tumour-associated macrophages are an essential component of the tumour microenvironment and have a role in the orchestration of angiogenesis…
"Tumour-associated macrophages are an essential component of the tumour microenvironment and have a role in the orchestration of angiogenesis…
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This is INCREDIBLE imaging to visualize the immune system attacking cancer cells. Congratulations to Alex Ritter for capturing this remarkable…
This is INCREDIBLE imaging to visualize the immune system attacking cancer cells. Congratulations to Alex Ritter for capturing this remarkable…
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Cell 'Debris' 🤔 | Garbage No More 👍 | Everything you need to know about #ExtracellularVesicles & their roles in the #ImmuneSystem | Edit Buzás…
Cell 'Debris' 🤔 | Garbage No More 👍 | Everything you need to know about #ExtracellularVesicles & their roles in the #ImmuneSystem | Edit Buzás…
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Pranjali Dalvi
Bachelor of Computer Engineering
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Hr Recruiter (MBA) #HumanResourseManagement #Recruitment #Recruiter #IT_Recruitement
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Pranjali Dalvi
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