Activity
-
It never hurts to “paws” and reflect on the day and what you hope tomorrow brings. (Or where you put that half-eaten salmon you were saving for…
It never hurts to “paws” and reflect on the day and what you hope tomorrow brings. (Or where you put that half-eaten salmon you were saving for…
Liked by Rui Li
-
Thank you, Miromatrix! Biotech is taking off in Minnesota. This one of a half dozen significant projects in the state right now. "Through…
Thank you, Miromatrix! Biotech is taking off in Minnesota. This one of a half dozen significant projects in the state right now. "Through…
Liked by Rui Li
-
Evia Bio is committed to delivering exceptional cryopreservation solutions tailored to your needs. From optimizing protocols and enhancing cell…
Evia Bio is committed to delivering exceptional cryopreservation solutions tailored to your needs. From optimizing protocols and enhancing cell…
Liked by Rui Li
Experience
Education
-
University of Minnesota-Twin Cities
-
Advisor: Allison Hubel, Ph.D.
Research topics: cryobiology, pluripotent stem cell biology -
-
-
-
Volunteer Experience
Publications
-
Differentiation of Human iPS Cells Into Sensory Neurons Exhibits Developmental Stage-Specific Cryopreservation Challenges
Frontiers in Cell and Developmental Biology
This investigation traces the cryobiological challenges of a multi-stage hiPSC-derived differentiation and manufacturing process. Low-temperature Raman spectroscopy and a series of cell assays revealed that cell cryobiology changed as the cells differentiated from hiPSCs via neural crest cells to sensory neurons. We identified cell dissociation and incubation before freezing as two pivotal non-cryopreserved steps influencing the success of cryopreservation. Our study correlated membrane…
This investigation traces the cryobiological challenges of a multi-stage hiPSC-derived differentiation and manufacturing process. Low-temperature Raman spectroscopy and a series of cell assays revealed that cell cryobiology changed as the cells differentiated from hiPSCs via neural crest cells to sensory neurons. We identified cell dissociation and incubation before freezing as two pivotal non-cryopreserved steps influencing the success of cryopreservation. Our study correlated membrane fluidity and cell size respectively with stage-specific sensitivity to nucleation temperature and cooling rate, which may be used to inform stage-specific cryopreservation designs to mitigate these sensitivities. This open-access article scrutinizes this manufacturing science from the combined perspective of cell development and cryobiology.
Other authorsSee publication -
Natural deep eutectic systems for nature‐inspired cryopreservation of cells
AIChE
Natural deep eutectic systems (NADES) are emerging as potential cryoprotective agents (CPA) for cell preservation. In this investigation, we develop an optimized CPA formulation using trehalose‐glycerol NADES (T:G) diluted in Normosol‐R and supplemented with isoleucine. The study also indicates that thermophysical properties of CPA solutions alone cannot predict optimal cell survival, suggesting that stabilization of biological structures by CPAs may play a role in successful cryopreservation.
Other authorsSee publication -
Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements
Cryopreservation and Freeze-Drying Protocols, 4th Edition, Springer
-
Cryopreservation of Human iPS Cell Aggregates in A DMSO-free Solution - An Optimization and Comparative Study
Frontiers in Bioengineering and Biotechnology
This open access article highlights a DMSO-free approach to cryopreserve iPS cells in the form of multicellular aggregates with little loss in cell quantity and function; a strategy to mitigate cryopreserved cells' vulnerability to undercooling (uncontrolled ice nucleation/seeding at low temperatures); low-temperature Raman spectroscopy evidence of synergistic effect of sugar, sugar alcohol, amino acid and P188.
Other authorsSee publication -
Preservation of cell-based immunotherapies for clinical trials
Cytotherapy
This article is a timely review of the state of cell-based immunotherapies. It addresses the key issues of interest that affect the quality of the cryopreserved cellular products, the current standard of practice that clinical studies have adopted, as well as the emerging technologies that aim to improve the preservation of these cellular therapeutics.
Other authorsSee publication -
Interfacial Interactions of Sucrose during Cryopreservation Detected by Raman Spectroscopy
Langmuir
This article is a part of Interfaces and Biology 1: Mechanobiology and Cryobiology special issue.
Other authorsSee publication -
Hydrophobic Patterning‐Based 3D Microfluidic Cell Culture Assay
Advanced Healthcare Materials
Physiologically relevant in vitro 3D human organ model featuring 3D endothelium, 95% interaction area between 3D ECM and microfluidic channel, chemotaxis gradient for study of interstitial cancer cell migration, high-multipotency 3D stem cell microenvironment.
Other authorsSee publication -
Freezing Responses in DMSO-Based Cryopreservation of Human iPS Cells: Aggregates Versus Single Cells
Tissue Engineering Part C: Methods
This study presented the significant difference between multicellular aggregates and single cells of the same cell type in response to freezing, the benefit of controlled-rate freezing with defined ice nucleation temperature, and an effective freeze-thaw protocol preserving the performance of human iPS cells at a level similar to standard passaging.
Other authorsSee publication
Courses
-
Advanced Tissue Engineering
-
-
Analytical Spectroscopy
-
-
Bio-nanoscience and Bio-nanotechnology
-
-
BioMEMS and Medical Microdevices
-
-
Biological Performance of Materials
-
-
Biomechanics
-
-
Biophotonics
-
-
Biostatistical Literacy
-
-
Cell and Tissue Engineering
-
-
Crystallography and Crystal Defects
-
-
Digital Signal Processing
-
-
Ethics in Biomedical Engineering
-
-
Ethics in Science and Engineering
-
-
Immunology
-
-
MN-REACH Value Proposition Design Workshop
-
-
Mass Transport in Drug Delivery
-
-
Materials Characterization
-
-
Materials Properties
-
-
Microelectronic Circuits
-
-
Microfluidics in Biology and Medicine
-
-
Physiology
-
-
Polymeric Biomaterials
-
-
Polymeric Materials
-
-
Probability and Risk Analysis in Industrial Operations Research
-
-
Quantitative Microscopy
-
-
Quantum Mechanics and Statistical Thermodynamics
-
-
Statistical Analysis
-
Projects
-
Studying the Complexity of Freezing Responses in DMSO-Based Cryopreservation of Human iPS Cell Aggregates
-
This cryopreservation study examined the difference between multicellular aggregates and single cells in responses to freezing, reported an effective preservation method of this cell type for future use in stem cell research, established metrics and assays to standardize the validation of preservation outcomes
Other creators -
A Novel Human Blood-Brain Barrier-on-Chip Model for Drug Screening
-
The in vitro model of human blood-brain barrier accurately recapitulated the key physiological characteristics of this tissue: intact endothelium with extremely low permeability, no spontaneous cell migration; providing high-throughput platform to screen drugs that need to be delivered across human blood-brain barrier during pre-clinical trials.
-
Genetically-engineered Mesenchymal Stem Cells for Rheumatoid Arthritis Treatment
-
A proposal of an injectable stem cell-hydrogel complex design that enhances immunomodulation with long-term stability for the treatment of stage II rheumatoid arthritis, including experiments for functional characterization and detailed pre-clinical evaluation to optimize the safety and efficacy of product, based on FDA guidelines and ISO10993
-
Self-assembling Vascular Graft Design
-
A low-cost high-throughput design proposal of vascular bypass graft based on the concept of peptide self assembly
-
Tissue-Engineering Vascular Graft Modification Design
-
An off-the-shelf vascular bypass graft design using tissue engineering approach and biomaterials modification, with a focus on in vivo re-endothelialization, aiming to prevent the occurance of acute thrombosis; functional characterization and pre-clinical testings designed for optimizing safety and efficacy of product based on FDA regulations
-
Effect of Red Wine on Expression of Lipid Metabolism Genes
-
A study showing that red wine polyphenolics and ethanol alter the expression of lipid metabolism genes in HepG2 cells; a critical discussion on the benefits of red wine on fatty liver disease.
-
Inhalable Lung Cancer Drug Delivery Design
-
A thorough design of a novel engineering drug delivery approach for treating non-small cell lung cancer; an integration of stable drug carrier transportation, tumor targeting, and sustained drug release
Honors & Awards
-
Walleye Tank Ice Fishing Edition Winner - Mid-level Reelers Division
-
Minnesota's community-based business competition focusing on life science innovations and providing opportunities for emerging and established medical and life science companies.
-
Minnesota Cup Grand Prize
-
Business competition hosted by MN Cup, a community-led, public-private partnership supporting Minnesota’s entrepreneurs.
-
Minnesota Cup Life Science/Health IT Division Winner
-
-
1st Place - SYIS Top Poster Award
TERMIS-Americas
A DMSO-Free Cryopreservation Strategy for Translational Stem Cell Technologies
-
Dean's Honors
UC Berkeley
Honors to 12-13 (Dean's Honors)
Honors to 12-11
Languages
-
English
Native or bilingual proficiency
-
Mandarin Chinese
Native or bilingual proficiency
Organizations
-
International Society for Cell and Gene Therapy
Co-chair, PDM Cold Chain Working Group
- Present -
American Society for Gene & Cell Therapy
-
- Present -
Society for Cryobiology
-
- Present -
International Society for Stem Cell Research
-
- -
Society for Laboratory Automation and Screening
-
- -
Tissue Engineering and Regenerative Medicine International Society
-
- -
Association of Laboratory Managers
-
- -
Women’s Health Leadership TRUST
-
-
More activity by Rui
-
If you encounter a bear in the woods, stay calm. Identify yourself by making noise so the bear knows you are a human. We recommend using your…
If you encounter a bear in the woods, stay calm. Identify yourself by making noise so the bear knows you are a human. We recommend using your…
Liked by Rui Li
-
PDA has just released the new revision of Technical Report No. 46, detailing guidance for last mile distribution. I served as a Peer Reviewer earlier…
PDA has just released the new revision of Technical Report No. 46, detailing guidance for last mile distribution. I served as a Peer Reviewer earlier…
Liked by Rui Li
-
Thank you to #ISBER and the #ISBERMontreal2025🍁 co-chairs Vanessa Fonseca Tumilasci, Daniel Catchpoole and Anusha Hettiaratchi for this opportunity…
Thank you to #ISBER and the #ISBERMontreal2025🍁 co-chairs Vanessa Fonseca Tumilasci, Daniel Catchpoole and Anusha Hettiaratchi for this opportunity…
Liked by Rui Li
-
Our very own scientists/professors propose plan to store bio samples on the moon! 🌕🌝 https://lnkd.in/gjRv8Bva #atpbio #NSFFunded #NSFERC…
Our very own scientists/professors propose plan to store bio samples on the moon! 🌕🌝 https://lnkd.in/gjRv8Bva #atpbio #NSFFunded #NSFERC…
Liked by Rui Li
-
Our Vial2Bag Advanced® admixture device, now available in 20mm and 13mm sizes, offers universal*compatibility with any IV bag brand*! Learn more…
Our Vial2Bag Advanced® admixture device, now available in 20mm and 13mm sizes, offers universal*compatibility with any IV bag brand*! Learn more…
Liked by Rui Li
-
Our latest work has been published Nature Magazine. We show how we can use hydrogels to store proteins without any #cold_chain . The proteins are…
Our latest work has been published Nature Magazine. We show how we can use hydrogels to store proteins without any #cold_chain . The proteins are…
Liked by Rui Li
-
Akshat Mallya just finished his oral presentation on cryopreservation of cardiomyocytes. Troy Louwagie will be giving his poster shortly on…
Akshat Mallya just finished his oral presentation on cryopreservation of cardiomyocytes. Troy Louwagie will be giving his poster shortly on…
Liked by Rui Li
-
Come check out our Poster at CRYO2024. #cryo2024 - via #Whova event app
Come check out our Poster at CRYO2024. #cryo2024 - via #Whova event app
Shared by Rui Li
-
I am speaking at CRYO2024. Please check out my talk if you're attending the event! #cryo2024 - via #Whova event app
I am speaking at CRYO2024. Please check out my talk if you're attending the event! #cryo2024 - via #Whova event app
Liked by Rui Li
-
Looking forward to hosting this exciting session on ice-free preservation technologies next week at #cryo2024. Dr. Gerald Brandacher, the…
Looking forward to hosting this exciting session on ice-free preservation technologies next week at #cryo2024. Dr. Gerald Brandacher, the…
Liked by Rui Li
-
Cryo2024 is almost here. What a great opportunity to learn about the latest advances in cryopreservation and connect with others in the field. Do…
Cryo2024 is almost here. What a great opportunity to learn about the latest advances in cryopreservation and connect with others in the field. Do…
Liked by Rui Li
Other similar profiles
Explore collaborative articles
We’re unlocking community knowledge in a new way. Experts add insights directly into each article, started with the help of AI.
Explore MoreOthers named Rui Li in United States
-
Rui Li
-
Rui Li
-
Rui Li
Investment Analyst at Columbia Investment Management Company
-
Rui Li
-
Rui Li
Graphics Software Engineer @ Mathworks
374 others named Rui Li in United States are on LinkedIn
See others named Rui Li