“I worked for about a year in close association with Rudra Dubey while he worked for an emerging Biotechnology Start-up, CellXplore. CellXplore is founded by Dr. Kiran Madura who was my previous postdoc supervisor and a Professor at the Department of Biochemistry, UMDNJ, New Jersey. Rudra drew upon his scientific expertise and skills and contributed remarkably to R&D activities at CellXplore. Throughout my contact with him, I noticed him working in team setting team and managing collaborative project that led to new discoveries. His key strengths are creativity and innovation, team work, multitasking, adhering to high standard of research and meeting deadlines. He is self-driven, takes up challenges and produces highest quality output. I have confidence that he would be an asset to any organization that he would a part of.”
Rudra Narayan Dubey, PhD, MBA
Guilford, Connecticut, United States
2K followers
500+ connections
About
Experienced professional, offering broad-base experience in multi-functional areas of…
Activity
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Welcome Bertrand - looking forward to doing great things together at Phraktion!
Welcome Bertrand - looking forward to doing great things together at Phraktion!
Liked by Rudra Narayan Dubey, PhD, MBA
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Leadership Theory - A leadership theory describes how and why certain individuals become leaders. The focus is on the characteristics and behaviors…
Leadership Theory - A leadership theory describes how and why certain individuals become leaders. The focus is on the characteristics and behaviors…
Liked by Rudra Narayan Dubey, PhD, MBA
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Today marks my first week in my new role as GM of Financial Services and Insurance at Domino Data Lab. I am already feeling the full Domino Effect.…
Today marks my first week in my new role as GM of Financial Services and Insurance at Domino Data Lab. I am already feeling the full Domino Effect.…
Liked by Rudra Narayan Dubey, PhD, MBA
Experience
Education
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Rutgers Business School, New Jersey, USA
Activities and Societies: 1. Member of Pharmaceutical Management Club, Rutgers Business School 2. Member of Rutgers Association of Marketing and Strategy Club, Rutgers Business School
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Publications
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Nucleoporin mediated nuclear positioning and silencing of HMR.
PLoS One. 2011;6(7):e21923. doi: 10.1371/journal.pone.0021923. Epub 2011 Jul 19
The organization of chromatin domains in the nucleus is an important factor in gene regulation. In eukaryotic nuclei, transcriptionally silenced chromatin clusters at the nuclear periphery while transcriptionally poised chromatin resides in the nuclear interior. Recent studies suggest that nuclear pore proteins (NUPs) recruit loci to nuclear pores to aid in insulation of genes from silencing and during gene activation. We investigated the role of NUPs at a native yeast insulator and show that…
The organization of chromatin domains in the nucleus is an important factor in gene regulation. In eukaryotic nuclei, transcriptionally silenced chromatin clusters at the nuclear periphery while transcriptionally poised chromatin resides in the nuclear interior. Recent studies suggest that nuclear pore proteins (NUPs) recruit loci to nuclear pores to aid in insulation of genes from silencing and during gene activation. We investigated the role of NUPs at a native yeast insulator and show that while NUPs localize to the native tDNA insulator adjacent to the silenced HMR domain, loss of pore proteins does not compromise insulation. Surprisingly we find that NUPs contribute to silencing at HMR and are able to restore silencing to a silencing-defective HMR allele when tethered to the locus. We show that the perinuclear positioning of heterochromatin is important for the NUP-mediated silencing effect and find that loss of NUPs result in decreased localization of HMR to the nuclear periphery. We also show that loss of telomeric tethering pathways does not eliminate NUP localization to HMR, suggesting that NUPs may mediate an independent pathway for HMR association with the nuclear periphery. We propose that localization of NUPs to the tDNA insulator at HMR helps maintain the intranuclear position of the silent locus, which in turn contributes to the fidelity of silencing at HMR.
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Interaction of APC/C-E3 ligase with Swi6/HP1 and Clr4/Suv39 in heterochromatin assembly in fission yeast.
J Biol Chem. 2009 Mar 13;284(11):7165-76. doi: 10.1074/jbc.M806461200. Epub 2008 Dec 30
Heterochromatin assembly in fission yeast is initiated by binding of Swi6/HP1 to the Lys-9-dimethylated H3 followed by spreading via cooperative recruitment of Swi6/HP1. Recruitment of Cohesin by Swi6/HP1 further stabilizes the heterochromatin structure and integrity. Subsequently, polyubiquitylation of Cut2 by anaphase-promoting complex-cyclosome (APC/C)-ubiquitin-protein isopeptide ligase (E3 ligase) followed by degradation of Cut2 releases Cut1, which cleaves the Rad21 subunit of Cohesin…
Heterochromatin assembly in fission yeast is initiated by binding of Swi6/HP1 to the Lys-9-dimethylated H3 followed by spreading via cooperative recruitment of Swi6/HP1. Recruitment of Cohesin by Swi6/HP1 further stabilizes the heterochromatin structure and integrity. Subsequently, polyubiquitylation of Cut2 by anaphase-promoting complex-cyclosome (APC/C)-ubiquitin-protein isopeptide ligase (E3 ligase) followed by degradation of Cut2 releases Cut1, which cleaves the Rad21 subunit of Cohesin, facilitating sister chromatid separation during mitosis. Here, we demonstrate a surprising role of APC/C in assembly of heterochromatin and silencing at mating type, centromere, and ribosomal DNA loci. Coincidentally with the loss of silencing, recruitment of Swi6, H3-Lys-9-Me2, and Clr4 at dg-dh repeats at cen1 and the K region of mat locus is abrogated in mutants cut4, cut9, and nuc2. Surprisingly, both Cut4 and Cut9 are also highly enriched at these regions in wild type and depleted in swi6Delta mutant. Cut4 and Cut9 interact directly with Swi6/HP1 and Clr4, whereas the mutant Cut4 does not, suggesting that a direct physical interaction of APC subunits Cut4 and Cut9 with Swi6 and Clr4 is instrumental in heterochromatin assembly. The silencing defect in APC mutants is causally related to ubiquitylation activity of APC-E3 ligase. Like swi6 mutant, APC mutants are also defective in Cohesin recruitment and exhibit defects like lagging chromosomes, chromosome loss, and aberrant recombination in the mat region. In addition, APC mutants exhibit a bidirectional expression of dh repeats, suggesting a role in the RNA interference pathway. Thus, APC and heterochromatin proteins Swi6 and Clr4 play a mutually cooperative role in heterochromatin assembly, thereby ensuring chromosomal integrity, inheritance, and segregation during mitosis and meiosis.
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Long-range communication between the silencers of HMR.
Mol Cell Biol. 2008 Mar;28(6):1924-35. doi: 10.1128/MCB.01647-07. Epub 2008 Jan 14.
Gene regulation involves long-range communication between silencers, enhancers, and promoters. In Saccharomyces cerevisiae, silencers flank transcriptionally repressed genes to mediate regional silencing. Silencers recruit the Sir proteins, which then spread along chromatin to encompass the entire silenced domain. In this report we have employed a boundary trap assay, an enhancer activity assay, chromatin immunoprecipitations, and chromosome conformation capture analyses to demonstrate that the…
Gene regulation involves long-range communication between silencers, enhancers, and promoters. In Saccharomyces cerevisiae, silencers flank transcriptionally repressed genes to mediate regional silencing. Silencers recruit the Sir proteins, which then spread along chromatin to encompass the entire silenced domain. In this report we have employed a boundary trap assay, an enhancer activity assay, chromatin immunoprecipitations, and chromosome conformation capture analyses to demonstrate that the two HMR silencer elements are in close proximity and functionally communicate with one another in vivo. We further show that silencing is necessary for these long-range interactions, and we present models for Sir-mediated silencing based upon these results.
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A tDNA establishes cohesion of a neighboring silent chromatin domain
Genes Dev. 2007 Sep 1;21(17):2150-60
DNA replication generates sister chromatid pairs that are bound to one another until anaphase onset. The process, termed sister chromatid cohesion, requires the multisubunit cohesin complex that resides at centromeres and sites where genes converge. At the HMR mating-type locus of budding yeast, cohesin associates with a heterochromatin-like structure known as silent chromatin. In this report, we show that silent chromatin is necessary but not sufficient for cohesion of the replicating locus. A…
DNA replication generates sister chromatid pairs that are bound to one another until anaphase onset. The process, termed sister chromatid cohesion, requires the multisubunit cohesin complex that resides at centromeres and sites where genes converge. At the HMR mating-type locus of budding yeast, cohesin associates with a heterochromatin-like structure known as silent chromatin. In this report, we show that silent chromatin is necessary but not sufficient for cohesion of the replicating locus. A tRNA gene (tDNA) that delimits the silent chromatin domain is also required, as are subunits of the TFIIIB and RSC complexes that bind the gene. Non-tDNA boundary elements do not substitute for tDNAs in cohesion, suggesting that barrier activity is not responsible for the phenomenon. The results reveal an unexpected role for tDNAs and RNA polymerase III-associated proteins in establishment of sister chromatid cohesion.
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Languages
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English
Full professional proficiency
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Hindi
Native or bilingual proficiency
Organizations
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aman gupta
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BBC in collaboration with BIRAC organized a virtual roadshow for Global Bio-India 2021 where speakers and participants had an insightful discussion…
BBC in collaboration with BIRAC organized a virtual roadshow for Global Bio-India 2021 where speakers and participants had an insightful discussion…
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Bridge Policy Think Tank and Bioinnovation Centre, Bangalore are hosting a webinar titled 'Global perspective on India’s Biotech Potential:…
Bridge Policy Think Tank and Bioinnovation Centre, Bangalore are hosting a webinar titled 'Global perspective on India’s Biotech Potential:…
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Business Process Drives Portfolio Management Strategy
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