Sabriya Stukes, PhD
New York, New York, United States
3K followers
500+ connections
About
Sabriya Stukes invests in early stage biotech companies that are solving human and…
Activity
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Today is #WorldPhotographyDay. Here’s the last photo on my camera roll. What’s yours?
Today is #WorldPhotographyDay. Here’s the last photo on my camera roll. What’s yours?
Liked by Sabriya Stukes, PhD
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Awesome panel! With a great conversation. Thanks all involved!
Awesome panel! With a great conversation. Thanks all involved!
Liked by Sabriya Stukes, PhD
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Feeling proud, humbled, and thrilled. When we launched ProteinDiscovery.AI, this is the exact kind of boundary-breaking, industry-supercharging…
Feeling proud, humbled, and thrilled. When we launched ProteinDiscovery.AI, this is the exact kind of boundary-breaking, industry-supercharging…
Liked by Sabriya Stukes, PhD
Experience
Education
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Albert Einstein College of Medicine of Yeshiva University
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Activities and Societies: Graduate Student Council Member, Quality of Life Graduate Student Representative, Ad-Libitum Assistant Editor, Einstein Journal of Biology and Medicine Assistant Editor, Albert Einstein College of Medicine Sue Golding Recruitment Representative, Graduate Reception Co-Chair, Board of Overseers PhD Student Representative
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Volunteer Experience
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Greater Opportunities Advancing Leadership & Science (GOALS) for Girls Mentor
Intrepid Sea, Air & Space Museum
- 9 months
Science and Technology
• Designed and demonstrated biomedical science workshops for approximately 50 students at the “GOALS Mentorship Day” and the “Girls in Science and Engineering Day”
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Einstein Summer Undergraduate Research Program (SURP) Mentor
Albert Einstein College of Medicine
- Present 11 years
• Worked with The Hispanic Center of Excellence to help two undergraduate students collect statistics and data via literature research to identify a disparity that affects an underrepresented population in the Bronx
• Research work culminated in the students formulating and presenting a proposal to an audience of faculty, students, and family to address the disparity within surrounding community
Publications
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Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy
Journal of Visualized Experiments
Many aspects of the infection of macrophages by Cryptococcus neoformans have been extensively studied and well defined. However, one particular interaction that is not clearly understood is non-lytic exocytosis. In this process, yeast cells are released into the extracellular space by a poorly understood mechanism that leaves both the macrophage and Cn viable. Here, we describe how to follow a large number of individually infected macrophages for a 24 hr infection period by time-lapsed…
Many aspects of the infection of macrophages by Cryptococcus neoformans have been extensively studied and well defined. However, one particular interaction that is not clearly understood is non-lytic exocytosis. In this process, yeast cells are released into the extracellular space by a poorly understood mechanism that leaves both the macrophage and Cn viable. Here, we describe how to follow a large number of individually infected macrophages for a 24 hr infection period by time-lapsed microscopy. Infected macrophages are housed in a heating chamber with a CO2 atmosphere attached to a microscope that provides the same conditions as a cell-culture incubator. Live digital microscopy can provide information about the dynamic interactions between a host and pathogen that is not available from static images. Being able to visualize each infected cell can provide clues as to how macrophages handle fungal infections, and vice versa. This technique is a powerful tool in studying the dynamics that are behind a complex phenomenon.
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Temporal kinetics and quantitative analysis of Cryptococcus neoformans nonlytic exocytosis.
Infection and Immunity
Cryptococcus neoformans is a facultative intracellular pathogen and the causative agent of cryptococcosis, a disease that is often fatal to those with compromised immune systems. C. neoformans has the capacity to escape phagocytic cells through a process known as nonlytic exocytosis whereby the cryptococcal cell is released from the macrophage into the extracellular environment, leaving both the host and pathogen alive. Little is known about the mechanism behind nonlytic exocytosis, but there…
Cryptococcus neoformans is a facultative intracellular pathogen and the causative agent of cryptococcosis, a disease that is often fatal to those with compromised immune systems. C. neoformans has the capacity to escape phagocytic cells through a process known as nonlytic exocytosis whereby the cryptococcal cell is released from the macrophage into the extracellular environment, leaving both the host and pathogen alive. Little is known about the mechanism behind nonlytic exocytosis, but there is evidence that both the fungal and host cells contribute to the process. In this study, we used time-lapse movies of C. neoformans-infected macrophages to delineate the kinetics and quantitative aspects of nonlytic exocytosis. We analyzed approximately 800 macrophages containing intracellular C. neoformans and identified 163 nonlytic exocytosis events that were further characterized into three subcategories: type I (complete emptying of macrophage), type II (partial emptying of macrophage), and type III (cell-to-cell transfer). The majority of type I and II events occurred after several hours of intracellular residence, whereas type III events occurred significantly (P < 0.001) earlier in the course of macrophage infection. Our results show that nonlytic exocytosis is a morphologically and temporally diverse process that occurs relatively rapidly in the course of macrophage infection.
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The F-Box protein Fbp1 regulates sexual reproduction and virulence in Cryptococcus neoformans.
Eukaryotic Cell
Cryptococcus neoformans is the leading cause of fungal meningitis in immunocomprised populations. Although extensive studies have been conducted on signal transduction pathways important for fungal sexual reproduction and virulence, how fungal virulence is regulated during infection is still not understood. In this study, we identified the F-box protein Fbp1, which contains a putative F-box domain and 12 leucine-rich repeats (LRR). Although fbp1 mutants showed normal growth and produced normal…
Cryptococcus neoformans is the leading cause of fungal meningitis in immunocomprised populations. Although extensive studies have been conducted on signal transduction pathways important for fungal sexual reproduction and virulence, how fungal virulence is regulated during infection is still not understood. In this study, we identified the F-box protein Fbp1, which contains a putative F-box domain and 12 leucine-rich repeats (LRR). Although fbp1 mutants showed normal growth and produced normal major virulence factors, such as melanin and capsule, Fbp1 was found to be essential for fungal virulence, as fbp1 mutants were avirulent in a murine systemic-infection model. Fbp1 is also important for fungal sexual reproduction. Basidiospore production was blocked in bilateral mating between fbp1 mutants, even though normal dikaryotic hyphae were observed during mating. In vitro assays of stress responses revealed that fbp1 mutants are hypersensitive to SDS, but not calcofluor white (CFW) or Congo red, indicating that Fbp1 may regulate cell membrane integrity. Fbp1 physically interacts with Skp1 homologues in both Saccharomyces cerevisiae and C. neoformans via its F-box domain, suggesting it may function as part of an SCF (Skp1, Cullins, F-box proteins) E3 ligase. Overall, our study revealed that the F-box protein Fbp1 is essential for fungal sporulation and virulence in C. neoformans, which likely represents a conserved novel virulence control mechanism that involves the SCF E3 ubiquitin ligase-mediated proteolysis pathway.
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Ectopic activation of Mycobacterium tuberculosis-specific CD4+ T cells in lungs of CCR7-/- mice.
Journal of Immunology
Initiation of an adaptive cellular immune response depends on intimate interactions with APCs and naive T lymphocytes. We previously reported that activation of naive Mycobacterium tuberculosis-specific CD4+ T cells depends on dendritic cell (DC) transport of live bacteria from the lungs to the mediastinal lymph node (MDLN). Because the migratory paths of DCs are largely governed by the chemokine receptor CCR7, which is expressed on DCs upon maturation by proinflammatory stimuli, we examined…
Initiation of an adaptive cellular immune response depends on intimate interactions with APCs and naive T lymphocytes. We previously reported that activation of naive Mycobacterium tuberculosis-specific CD4+ T cells depends on dendritic cell (DC) transport of live bacteria from the lungs to the mediastinal lymph node (MDLN). Because the migratory paths of DCs are largely governed by the chemokine receptor CCR7, which is expressed on DCs upon maturation by proinflammatory stimuli, we examined the quantitative contribution of CCR7-dependent DC migration in the context of tuberculosis. We found that early trafficking of DCs from the lungs to the MDLN depended on CCR7-mediated signaling, but alternative mechanism(s) are used later in infection. Impaired migration of DCs in CCR7(-/-) mice resulted in delayed dissemination of bacteria to MDLN and spleen and in delayed kinetics of activation of adoptively transferred Ag85B-specific CD4+ T cells. Furthermore, in contrast to control mice, we found that naive Ag85B-specific CD4+ T cells are activated to proliferate in the lungs of CCR7(-/-) mice and, when infected with higher doses of bacteria, resistance to M. tuberculosis infection in CCR7(-/-) mice is compromised compared with wild-type mice.
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The locus encoding an oligomorphic family of MHC-A alleles (Mane-A*06/Mamu-A*05) is present at high frequency in several macaque species.
Immunogenetics
Several macaques species are used for HIV pathogenesis and vaccine studies, and the characterization of their major histocompatibility complex (MHC) class I genes is required to rigorously evaluate the cellular immune responses induced after immunization and/or infection. In this study, we demonstrate that the gene expressing the Mane-A*06 allele of pig-tailed macaques is an orthologue of the locus encoding the Mamu-A*05 allele family in rhesus macaques. Analysis of the distribution of this…
Several macaques species are used for HIV pathogenesis and vaccine studies, and the characterization of their major histocompatibility complex (MHC) class I genes is required to rigorously evaluate the cellular immune responses induced after immunization and/or infection. In this study, we demonstrate that the gene expressing the Mane-A*06 allele of pig-tailed macaques is an orthologue of the locus encoding the Mamu-A*05 allele family in rhesus macaques. Analysis of the distribution of this locus in a cohort of 63 pig-tailed macaques revealed that it encodes an oligomorphic family of alleles, highly prevalent (90%) in the pig-tailed macaque population. Similarly, this locus was very frequently found (62%) in a cohort of 80 Indian rhesus macaques. An orthologous gene was also detected in cynomolgus monkeys originating from four different geographical locations, but was absent in two African monkey species. Expression analysis in pig-tailed macaques revealed that the Mane-A*06 alleles encoded by this locus are transcribed at 10- to 20-fold lower levels than other MHC-A alleles (Mane-A*03 or Mane-A*10). Despite their conservation and high prevalence among Asian macaque species, the alleles of the Mane-A*06 family and, by extension their orthologues in rhesus and cynomolgus monkeys, may only modestly contribute to cellular immune responses in macaques because of their low level of expression.
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Honors & Awards
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2014 Federation of American Societies for Experimental Biology (FASEB) BioArt Winner
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Organizations
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New York Women in STEM
Advocacy Committee Co-Chair
- Present• Collected data from over 1,000 graduate students and postdoctoral fellows to assess how different institutional policies and culture affect research for women in NYC • Acted as a liaison between the NYWiSTEM Executive Board and the Albert Einstein College of Medicine to promote scientific events and increase membership
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NYC Minority Graduate Student Network
Director of Events, Member of the Executive Board
- PresentThe NYC Minority Graduate Student Network is student-run organization founded in 2006 with the purpose of fostering and supporting the next generation of minority scientists.We operate in collaboration with the Center for Translational and Basic Research (CTBR) at CUNY Hunter College and the New York Academy of Sciences (NYAS) to organize and promote career and personal development workshops, networking events, and mentoring and outreach opportunities for all of our members. As Director of…
The NYC Minority Graduate Student Network is student-run organization founded in 2006 with the purpose of fostering and supporting the next generation of minority scientists.We operate in collaboration with the Center for Translational and Basic Research (CTBR) at CUNY Hunter College and the New York Academy of Sciences (NYAS) to organize and promote career and personal development workshops, networking events, and mentoring and outreach opportunities for all of our members. As Director of Events, my responsibilities are as follows: • Organized and coordinated numerous career development and science workshops for the underrepresented graduate student community in New York City • Helped solidify a newly appointed Executive Board, increased organization awareness to surrounding institutions and spearheaded the creation a functional website • Acted as a liaison between the MGSN Executive Board and the Albert Einstein College of Medicine to promote career development workshops and scientific events as well as increase membership
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Einstein Minority Scientists Association
Executive Board Member
- Present• Provided workshops to prepare first and second year doctoral candidates for their qualifying exams • Hosted special guest lectures for our faculty members and graduate student body • Established working relationships to engage scientists with neighboring middle schools and high schools • Worked closely with the graduate department to enhance the Post-baccalaureate Research Education Program (PREP) which aims to encourage individuals from underrepresented communities to apply for graduate…
• Provided workshops to prepare first and second year doctoral candidates for their qualifying exams • Hosted special guest lectures for our faculty members and graduate student body • Established working relationships to engage scientists with neighboring middle schools and high schools • Worked closely with the graduate department to enhance the Post-baccalaureate Research Education Program (PREP) which aims to encourage individuals from underrepresented communities to apply for graduate level degrees by organizing monthly journal clubs, and facilitating meetings between students and faculty for participants
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