Multiplexing in #singlecellproteomics can substantially increase throughput, but requires flexible and easy to implement, scalable and sensitive sample preparation strategies for highest data quality. In collaboration with the Slavov Lab, we demonstrate in this application note, an nPOP workflow that can accommodate different numbers of labels and different reagents simply by programming different configurations of droplets deposited on the surface of glass slides. nPOP with non-isobaric tags, or plexDIA approach, is providing an automated option for scaling up single-cell proteomics for analysis of thousands of single cells with proteome depths comparable to label-free DIA applications. We applied plexDIA in a fully automated 3-label approach on CFPAC-1 pancreatic cancer, WM-989 melanoma, and THP-1 leukemia cell lines (126 single cells in total) using the timsTOF Ultra in dia-PASEF mode. This approach allowed to analyze almost 100 samples per day with a proteome depth of more than 3000 proteins per cell (4500 in total) and quantification of > 80,000 precursors per individual plexDIA run. #TIMS separation aided in complexity reduction by MOMA. Intercalated MS1 scans in the dia-PASEF method increased the average number of data points per peak to 14.6 increasing quantitative accuracy. Read more: https://lnkd.in/euyegGUw #massspectrometry #proteomics #timsTOF
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Curious about Bioimpedance Spectroscopy (BIS)? Not sure how it works or why it’s crucial in breast cancer care? Discover the science behind BIS and its impact on early lymphedema detection and prevention in breast cancer patients. 👉 Learn more: https://ow.ly/eGGj50QbMkE 📽️ Watch our informative video to see BIS in action Empower yourself with knowledge and join us in the fight against breast cancer-related lymphedema. #BIS #BioimpedanceSpectroscopy #BreastCancerCare #EarlyDetection #LymphedemaPrevention
What is BIS?
impedimed.com
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AAT Bioquest iFluor™ #dyes span the UV-visible to near-infrared #fluorescent spectrum and exhibit superior brightness and photo-stability to Alexa Fluor® for all your #immunofluorescence assays, cell imaging and #flowcytometry applications. #conjugation #labeling 🔗 https://lnkd.in/eW-Eq_mH
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Explore the latest in our Drug Discovery Dialogues series. In this video interview by SelectScience®, uncover how high-content live cell imaging solution and machine learning assays are shaping the future of drug discovery. Hope Amos reveals how Nanolive SA's unique imaging technology that uses holotomography. She says "The holography part is that we pass a low-power light source that doesn't affect cells or cause phototoxicity through the sample...The tomography part involves taking 96 slices in the Z-stack. This allows us to look inside cells and see the organelles at high resolution and by combining both of these techniques we have high resolution in 3D ." Hear how these assays are being developed to identify #MetabolicStress in the absence of cell death. Watch it now: https://lnkd.in/eg-pUH48 #DrugDiscoveryDialogues #Holotomography #Cytotoxicity #CellImaging
Drug Discovery Dialogues | SelectScience explores unique holotomography technology with Hope Amos, Nanolive SA
selectscience.net
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Check out my interview with SelectScience® in case you missed the opportunity to stop by Nanolive's booth or Tech Talk at the ELRIG UK Drug Discovery Conference. I explain how Nanolive SA's unique imaging & analysis technology works, and how it can be used for novel drug discovery insights, in under 5 mins. #livecellimaging #drugdiscovery #metabolism #cellbiology
Explore the latest in our Drug Discovery Dialogues series. In this video interview by SelectScience®, uncover how high-content live cell imaging solution and machine learning assays are shaping the future of drug discovery. Hope Amos reveals how Nanolive SA's unique imaging technology that uses holotomography. She says "The holography part is that we pass a low-power light source that doesn't affect cells or cause phototoxicity through the sample...The tomography part involves taking 96 slices in the Z-stack. This allows us to look inside cells and see the organelles at high resolution and by combining both of these techniques we have high resolution in 3D ." Hear how these assays are being developed to identify #MetabolicStress in the absence of cell death. Watch it now: https://lnkd.in/eg-pUH48 #DrugDiscoveryDialogues #Holotomography #Cytotoxicity #CellImaging
Drug Discovery Dialogues | SelectScience explores unique holotomography technology with Hope Amos, Nanolive SA
selectscience.net
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🔬🌈 Dive into the world of biomolecular condensates with CoraLite® Conjugates! CoraLite conjugates facilitate: - Unrivalled Specificity - Crystal-clear imaging - Amplified sensitivity - A simplified workflow #biomolecularcondensates #immunofluorescence #microscopy #multiplexing #coralites #phaseseperation
Learn more: CoraLite® conjugated antibodies
ptglab.com
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💪 Get going and don't give up ! Every year I make good resolutions, so to help you get creative, I suggest you take inspiration from the WHO recommendations. The World Health Organisation has published a study which estimates that 80% of cases of heart disease, stroke and type 2 diabetes could be prevented by adopting six daily habits. Estimates are estimates, but anything approaching an 80% improvement is worth changing. These five habits are 1. Eat whole, plant-based foods every day 2. Get at least 6 hours' sleep a day (ideally 7 to 9 hours) 3. Exercise for 150 minutes a week 4. Avoid tobacco and alcohol 5. Give priority to social interaction As you can see, the majority don’t require money — just commitment and consistency !! 😉 Personally, I've got 3 out of 5 that I've managed to keep for a few years now...
🌟All the team would like to wish you all the best for 2024. 🌟 This new year holds many challenges in store for us, as well as some exciting new projects and successes. We invite you to follow us, meet us, support us and take part in our developments. Tissue engineering and cell therapy are our near future !
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Voxcan, part of the ERBC GROUP, specializes in non-invasive high-resolution imaging for non-clinical drug development. We offer advanced preclinical biodistribution studies using in vivo fluorescence imaging. ➡️ Our techniques enable real-time observation of labelled compounds' biodistribution with optimized NIR fluorochromes in both naïve and disease models. Combining bioluminescence with fluorescence imaging, we provide insights into compound colocalization with pathological processes over time. #3Rs #biodistribution #drugdevelopment #NAM #bioluminescence #fluorescenceImaging
📢 ERBC offers advanced preclinical biodistribution studies using non-invasive in vivo fluorescence imaging. Our experienced team has experience in following a wide range of test items: ✅Nanoparticles ✅Nanomaterials ✅Proteins ✅Antibodies ✅Liposomes This technique allows to follow labelled-compounds biodistribution in live animals over time, using optimized NIR fluorochromes in both naïve and disease models. By combining bioluminescence with fluorescence imaging, we can assess compound colocalization with pathological processes longitudinally. Enhance your drug development with our state-of-the-art imaging technologies! #3Rs #biodistribution #drugdevelopment #NAM #bioluminescence #fluorescenceimaging
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Google and Calico Labs use our Gas Mixer: How to adjust the O2 concentration on the fly to achieve target blood oxygen levels. Read the full article: Hypoxia in Preclinical Imaging https://lnkd.in/de6SEYz
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Google and Calico Labs use our Gas Mixer: How to adjust the O2 concentration on the fly to achieve target blood oxygen levels. Read the full article: Hypoxia in Preclinical Imaging https://lnkd.in/de6SEYz
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Cell culture lies at the heart of many biological studies, serving as a foundational technique for a myriad of applications, from basic research to drug discovery. Register for this exciting webinar that will explore the newest features and instrument capabilities supporting kinetic live-cell imaging, from real-time analysis of rapid GPCR signaling pathways to long-term treatment-induced effects on cell proliferation and viability. Register here: https://buff.ly/3JbyFXv #ChemetrixAcademy #CellCulture #CellAnalysis #LaboratoryAnalysis #Webinar #Imaging #AnalyticalInstruments
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