Michael McKeown’s Post

Here's our latest review on combinatorial biosynthesis approaches in fungi to design new bioactive molecules: https://lnkd.in/gEWPSqD3

🔬 Discover an accurate and robust method for your microbial metabolism research with SUPER SESI-HRMS. 💡 Integrating the dGOT-SESI-HRMS with the microbial-VOC database and spectral stitching techniques, Jiangjiang Zhu, Kelly Meyrath, Rui Xu and Fouad Choueiry have significantly enhanced metabolite detection in anaerobic bacterial cultures. Their comprehensive analysis, employing a combination of full scan DDA, conventional GOT, and dGOT methods, has yielded more accurate and reliable results. 🔍 The team achieved proportional fragmentation of peaks across various analysis windows using dGOT-SESI-HRMS, identifying 109 VOCs from 306 targeted compounds. This method has not only improved the annotation of unique volatiles but also provided deeper biological insights through principal component analysis. 🧬 This breakthrough represents a major advancement in the monitoring of microbial metabolism, offering a more robust and reliable analytical tool compared to traditional methods. See full article here: https://lnkd.in/dciT_U28

Partial purification and characterization of a gibberellin-binding protein from seedlings of Azukia angularis https://t.co/WQl0CDEjfp #EurekaMag

This video is for practicals done in recombinant DNA technology . This video content an series of experiments: 1. Isolation of plasmid DNA by alkaline lysis method 2. Restriction Digestion of pUC 19 using EcoRI enzyme 3. Double digestion of pUC1 19 using BamHI and Ssp1 enzymes 4. Quantification of purified digested DNA fragment 5.Blue White Screening 6. GFP cloning Video credit goes to us Sujata Pinjarkar Pratik Kumbhare Bandana Verma These all practicals were done in DYPIU lab under the guidance of Priyatosh Ranjan Sir Thankyou for your valuable guidance and supporting us. #dypiu #RecombinantDNATechnology #DNA #laboratory #pune #biotechnology #medical Hope you like this video😊 https://lnkd.in/dV4yM4mq

Glycosylation is one of the most impactful modifications found in biologic systems, making glycobiology profiling critical to many areas of research. Click to learn how we can help you fill your toolbox with glycobiology reagents and tools like enzymes, resins, labels, and reagents to aid in the discovery and experimentation around glycobiology.

🌟 Thrilled to share our latest work on enzyme evolution and epistasis in #NatureCatalysis ! Our research has revealed a fascinating aspect of mutation interplay. While some mutations individually might not make a big splash, together, they create an amplified effect on resistance development. This synergy can be due to altering the very mechanics of reaction processes by orthogonally enhancing substrate binding and the chemical step. Understanding how epistasis is linked to a shift in the rate-limiting step of enzyme activity may open new doors not only for enzyme design but also for innovative drug development strategies. https://lnkd.in/dF4Wkrd2  #Epistasis #antibioticresistance #Biotechnology #DrugDevelopment #Innovation

🚀 Enhancing Protein Stability with Amino Acids and the #CrystalBreeder🌟 We are excited to share the application of #CrystalBreeder in a recent research work on "Amino Acids Stabilizing Effect on Protein and Colloidal Dispersions". In this study, researchers discovered that #AminoAcids (AAs) possess a broad colloidal stabilizing property, effective across various #proteins, plasmid DNA, and even non-biological nanoparticles. The CrystalBreeder was instrumental in measuring the cloud point of #lysozyme solutions demonstrating the ability of AAs to alter the cloud point by up to 4 K. In the CrystalBreeder instrument, samples were equilibrated at 42 °C for 10 minutes, then cooled at 0.2 °C/min under nitrogen flow to prevent oxidation. The cloud point temperature was recorded at 70% transmissivity loss. 🔬 Key insights from the research: ➡ Charge Interactions: Charged AAs #stabilize proteins of opposite charge. ➡Peptide Efficiency: Short #peptides are as effective as individual AAs. ➡Surface Solvation: Small molecules enhancing surface solvation can stabilize colloids. The data indicate that higher intracellular concentrations of amino acids and peptides will impact all protein-protein interactions. Learn more about the experiment and findings: https://lnkd.in/dPu9Du9W #Technobis #HelpResearchSucceed #Biotechnology #Colloids #Protein #Stabilization #AminoAcids #Peptides #Solvation #Nanoparticles #CloudPoint

Exploring the Catalase Activity of Escherichia coli (E. coli). Requirements: - Media: Sterile nutrient broth tube - Culture: E. coli - Reagent: Methylene blue solution Protocol: 1. Inoculate a loopful of E. coli culture in a nutrient broth tube. 2. Incubate the tube at 37°C for 24 hours. 3. After incubation, add 1-2 drops of methylene blue solution. 4. Incubate the tube at 37°C for 30 minutes. 5. Record the results. Results: - Before: The nutrient broth appears clear. - After: The nutrient broth appears cloudy due to the growth of E. coli and the catalase activity. Extra Information: Catalase is an essential enzyme found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen, a crucial reaction for protecting cells from oxidative damage. In this experiment, the presence of catalase activity in E. coli can be inferred from the color change induced by the methylene blue solution. The color change indicates the breakdown of hydrogen peroxide, confirming the enzymatic activity. Why is this important? Understanding catalase activity in bacteria like E. coli is critical in microbiology and biotechnology. It helps in comprehending bacterial metabolism and the mechanisms they use to survive in aerobic conditions. This knowledge can be applied in various fields, from clinical diagnostics to industrial microbiology. #Microbiology #Biotechnology #EColi #CatalaseActivity #LabWork #Research #Science #Biology #Enzymes #BacterialMetabolism #ClinicalDiagnostics #IndustrialMicrobiology #NikhilVishwakarma

Protein ligand complex simulations | 5 Day Technical Hands-on certificate workshop is starting tomorrow. Register now from the LINK IN BIO or https://lnkd.in/gRSdJbeQ #bdglifesciences #proteinligandcomplex #molecularsimulations #moleculardynamics #proteinstructure #ligandbinding #computationalbiology #bioinformatics #proteininteractions #molecularmodeling #proteinfunction #drugdiscovery #proteinengineering #proteinanalysis #molecularinteractions #proteinbinding #computationalchemistry #molecularbiology #proteinresearch #liganddocking #molecularsimulation

Discover Precision in Action! Explore our Erba cholesterol kit from the biochemistry product line. Find out how our kits ensure pinpoint accuracy and reliability. Step into the future of biochemistry with Erba! #WeAreErba #Biochemistry #CholesterolKit #Diagnostics #Innovation