Miltenyi Biotec’s Post

Molecular unmixing of a healthy, hairless mouse using the TriTom preclinical imaging system from PhotoSound. The single wavelength 1064 nm reconstruction shows high-resolution vascular structures in both superficial and deep tissue. The relative concentrations of oxy- and deoxyhemoglobin were then resolved using a linear spectral unmixing algorithm and used to generate a molecular map of oxygen saturation. For more information visit www.photosound.com #molecularimaging #molecularbiology #preclinical

Lipid analysis by PRM-SRS microscopy --- Understanding lipids' involvement in aging and various diseases requires mapping their spatial distributions and examining metabolic dynamics. Traditional imaging methods, like imaging mass spectrometry, fluorescence labeling, and optical imaging, may change cell environments, have limited resolution, or fail to distinguish lipid subtypes. In a recent study, researchers introduced a hyperspectral imaging platform, combining the Penalized Reference Matching algorithm with Stimulated Raman Scattering (PRM-SRS) microscopy. This approach allowed to observe a high cholesterol to phosphatidylethanolamine ratio in mouse hippocampus granule cells and analyze subcellular distributions of sphingosine and cardiolipin in the human brain. In this case, Lipotype Lipidomics technology was used as a reference method for lipid quantification. Read the full publication: https://lnkd.in/esq9uiS6 #research #lipid #lipidomics #LipidMetabolism #lipotype #lipidome #MassSpectrometry

After our Nature methods (https://lnkd.in/gD4aS8X) and nature protocols (https://lnkd.in/egQAPyp) papers, our third Nature-series paper based on FMP-10 derivatization and mass spectrometry imaging of neurotransmitters is out (https://lnkd.in/e5ZQS_aY). The spatial omics approach combines histology, mass spectrometry imaging and spatial transcriptomics to facilitate precise measurements of mRNA transcripts and neurotransmitters across tissue regions. #FMP10 #Tagon #parkinsonsdisease #massspectrometry #reactivematrix #transcriptomics #metabolomics #spatialanalysis

Struggling to capture microscopy images that meet your standards? 🔬 We spoke with KOLs at Cell Signaling Technology (CST) and Jackson ImmunoResearch to better understand the key considerations for optimizing fluorescence microscopy protocols for high-quality imaging. Here are some of our insights from our latest article: 1️⃣ Optimize your fixation method for the locations of your targets 2️⃣ For intracellular detection, consider using your permeabilizing agent in your blocking and antibody staining buffers 3️⃣ Select fluorophores with greater Stokes shifts to prevent unwanted background fluorescence https://lnkd.in/gjhkvqem #immunohistochemistry #immunofluorescence #fluorescence #microscopy #immunoassay

Large organoid and cell culture imaging of cardiomyocytes from Scott Page with a massive field of view and high frame rate imaging. Imaging multiple wells or 9 x 9mm organoids in one field of view! #LMA2024

📍 Comparison of spatial transcriptomics technologies used for tumor cryosections Rademacher et al. conducted an imaging spatial transcriptomics (iST) of the same medulloblastoma with enhanced nodularity (MBEN) samples by Molecular Cartography, Merscope and Xenium in comparison to RNAscope HiPlex alongside sequencing-based ST on Visium slides.   Key points ✔ Very well performance of all iST methods in terms of their sensitivity and specificity in our case study ✔ Very similar pictures of the MBEN tissue microanatomy and cellular neighborhoods with the spatial distribution of cell types annotated based on the shared set of 96 genes ✔ Selection of platforms based on the criteria arisen from differences in the technology and their implementation as well as associated requirements for the practical work. ➡ More details: https://lnkd.in/gaZjzyCu #GermanCancerResearchCenter #spatialomics #spatialbiology #singlecellanalysis #singlecell

First research paper is out! 🔬 Addressing live oxygen imaging in 3D models using deep red/NIR nanosensors which can be used in ratiometric analysis or PLIM Thanks to all co-authors (Irina Okkelman, Nina Heymans and Ruslan Dmitriev), collaborations (Prof. Sergey Borisov @TUGraz, #LECR and #VIB) and reviewers for the support. Access the paper on ASC Nano (https://lnkd.in/eQMySR5D)

There are exciting new developments in brain imaging that can help us better understand energy metabolism and investigate brain tumors, using our stable isotopes. Read the newly published open-access NeuroImage paper here.

Imaging organoids can be challenging, mainly due to phototoxicity and equipment sensitivity. In our new blog, we want to bring line rescanning confocal imaging to your attention. Would you like to know how it can benefit your organoid imaging? Start reading now, for brand new insights.

Functionalization of Radiolabeled Antibodies to Enhance Peripheral Clearance for High Contrast Brain Imaging Article by Eva Schlein, PhD et al., using #Mediso nanoScan® SPECT/CT system. #imaging #preclinical #molecularimaging #antibodies #brainresearch