Molecular Assemblies, Inc.’s Post

#snsinstitutions #snsdesignthinkers #designthinking PROTEIN Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, responding to stimuli, providing structure to cells and organisms, and transporting molecules from one location to another. Proteins differ from one another primarily in their sequence of amino acids, which is dictated by the nucleotide sequence of their genes, and which usually results in protein folding into a specific 3D structure that determines its activity.

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https://lnkd.in/dqWBjd2A. What is recoding DNA? In recoding an organism, a codon, or sequence of three DNA or RNA nucleotides involved in making proteins, is replaced with a synonymous alternative codon that maintains genome function and has the potential to produce new amino acids, the building blocks of proteins.

Sanshibio Taq DNA Polymerase is an improved and screened Taq DNA polymerase. In addition to the characteristics of the traditional enzyme itself, it also has broader advantages after repeated testing. First, Sanshibio's Taq enzyme is equipped with a basic universal buffer, which has a good amplification effect on common plant, animal, microbial, etc. DNA or cDNA; it is compatible with the buffers of most mainstream manufacturers on the market; the modified The enzyme combined with the basic universal buffer can achieve stronger resistance, and can tolerate DNA crude extracts containing a large amount of impurities; it has stronger stability and can tolerate multiple freeze-thaw cycles; for some low-abundance templates, it can be used The amount of enzyme added is reduced, and the amount of magnesium ions is appropriately increased to achieve more sensitive detection. https://lnkd.in/gnYiU5VJ

For several decades, plasmid isolation has been pretty much standard, following resuspension (often includes RNA degradation with RNase), alkaline lysis, neutralization, separation of cell debris, washing, and elution. Moreover, this procedure comes in larger scales of preparation, namely: midiprep, maxiprep, megaprep, and gigaprep. What sets the differences among these scales of preparation (including miniprep) are as follows: 1. Culture volume where plasmids will be extracted 2. Yield Meanwhile, a commendable practice in preparing cultures for plasmid isolation involves overnight 37°C incubation of bacterial culture with a certain speed of orbital shaking. It provides aeration during the incubation period. With aeration in a form of shaking, better growth, better yield. Also, we have to take note of the copy number of the plasmid we are trying to isolate. Definitely, it is convenient to obtain high yields for plasmids with high copy numbers. On the other hand, there are ways to circumvent the challenge in low copy number plasmids. So we have to understand the nature of the plasmids, even the selectable marker (e.g. antibiotic resistance gene/s) they convey. Surely, plasmid isolation is a significant step in cloning as it is our means to acquire vectors in large quantities.

PacBio sequencing of human fecal samples uncovers the DNA methylation landscape of 22 673 gut phages https://lnkd.in/dmrvedjr

Our ready-to-use Proteinase K Solution provided in a 20 mg/mL form is a broad-spectrum serine protease with very high specific activity. It is active under a wide range of reaction conditions, including elevated temperatures and in the presence of SDS. • No thawing or resuspension needed before use   • Retains greater than 80% activity up to 60°C • Low residual DNA content Proteinase K in a lyophilized powdered form is also available. Learn more about our Proteinase K and Proteinase K Solution: #DNA #Genotyping #MolecularBiology #PoweringBetterPCR

From unmodified sequences to peptides containing modified residues, our histone collection enables you to explore the diverse range of histone modifications, including acetylation, methylation, phosphorylation and more. We offer five different histone peptide sets available in 3 different formats: ➡ 94 different unbound peptides in deep well tray format ➡ 94 different biotinylated peptides immobilised in 96 well plate format ➡ 94 different peptides printed in duplicate as micro array slides To order, visit shop.altabioscience.com or contact our scientists at [email protected] for further information. #epigenetics #dna #genetics #dnamethylation #lifesciences #histones

Researchers have a general understanding of the role of DNMT1 in the methylation maintenance mechanism. However, there is still a lack of precise quantitative studies on DNMT1's preference. Novogene's high-quality sequencing services supported the precise quantitative research of the methylation mechanism and provided a comprehensive preference profile. 👉https://ow.ly/sX0350Qmq6g #DNAmethylation #Epigenetics #NGS

Codons, nucleotides and amino acids explained Codon: a sequence of three nucleotides which together form a unit of genetic code in a DNA or RNA molecule. Nucleotides are molecules consisting of a nucleoside and a phosphate group. They are the basic building blocks of DNA and RNA. Amino acids are organic compounds that combine to form proteins. Amino acids and proteins are the building blocks of life. When proteins are digested or broken down, amino acids are left. The human body uses amino acids to make proteins to help the body: Break down food. Youtube video: https://lnkd.in/dH-errVK #nikolaysgeneticslessons