Molecular Assemblies, Inc.’s Post

A University of Sydney team led by Sandro Ataide have developed a superior gene editing tool set to the current industry standard CRISPR. "SeekRNA uses a programmable ribonucleic acid (RNA) strand that can directly identify sites for insertion in genetic sequences, simplifying the editing process and reducing errors." https://lnkd.in/g4_5wakt #biology #genetics #geneticengineering #biotech #biotechnology #molecularbiology #cellbiology #biochemistry #computationalbiology

🔬 Did you know that selecting the appropriate competent strain can help maintain and purify the integrity of plasmids with extended poly(A) tails? 🌟 Explore how genetically engineered strains are revolutionizing molecular biology with cutting-edge technologies and innovative research. ✔️Discover advanced gene editing and high-throughput screening driving the creation of high-quality engineered strains. ✔️Essential for Research: Understand why researchers need to engineer strains for improved DNA cloning, plasmid construction, and protein expression in molecular biology. ✔️Learn how GenScript’s Poly(A) Strain V2/V3 enhances stability and efficiency, ensuring high-quality results in genetic research. 👉Read Now: https://ow.ly/6f3W50Savfy #cloning #strain #plasmid #molecularbiology #RelibailityisinourGENES

#researchinnovation Exciting Discovery Alert from #Science 🔬 Ribozymes, the RNA-based enzymes known for self-cleavage and splicing, are breaking new ground! Researchers from Tsinghua University reveal the emergence of hydrolytic endonucleolytic ribozymes (HYERs), capable of precise DNA manipulation. These proteins operate sans proteins, revolutionizing gene editing in bacteria and human cells, potentially rivaling CRISPR. Some HYERs adopt a homodimer structure, recognizing DNA via a complementary target sequence and hydrolytically cleaving it. Through reprogramming, authors redirect HYERs to new DNA targets, enhancing precision and versatility. Dive deeper into this groundbreaking discovery here: https://lnkd.in/dVfDsSmj #genetics #research #Ribozymes #geneediting #crispr #science #researchanddevelopment #scienceandtechnology #scienceofreading #researchpaper #biotech #biotechnology

Very interesting findings about unintended insertions of viral vectors when using AAVs for #crispr #crisprcas9 #geneediting #genomeediting #genetherapy #rnatherapeutics

𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡𝐞𝐫𝐬 𝐝𝐢𝐬𝐜𝐨𝐯𝐞𝐫 𝐮𝐧𝐞𝐱𝐩𝐞𝐜𝐭𝐞𝐝 𝐨𝐮𝐭𝐜𝐨𝐦𝐞 𝐨𝐟 𝐠𝐞𝐧𝐨𝐦𝐞 𝐞𝐝𝐢𝐭𝐢𝐧𝐠 𝐰𝐢𝐭𝐡 𝐂𝐚𝐬9 𝐚𝐧𝐝 𝐀𝐀𝐕 Researchers in the US, Japan and the UK report in Nature Biotechnology that genome editing with Cas9 and adeno-associated virus repair templates leads to frequent insertion of viral vectors that are difficult to detect, and that these events occur regardless of locus, vector concentration, cell line or cell type. Read more at CRISPR Medicine News 👇🏻 https://lnkd.in/e5Erfgjn #crisprmedicinenews #crisprmedicine #crispr #genomeediting #geneediting #genomeengineering The team was led by Professors Ravi Majeti (Stanford University School of Medicine) and Hiromitsu Nakauchi Hiro (@Stanford University School of Medicine and The University of Tokyo), and involved researchers from Japan Society for the Promotion of Science, Stanford University and University of Oxford.

CRISPR 2.0: a new wave of gene editors heads for clinical trials. Landmark approval of the first CRISPR therapy paves the way for treatments based on more efficient and more precise genome editors. Read: https://lnkd.in/eVmNSrUy #sciencesdelavie #biotechnologie #biotechnology #innovation #recherche #sciences #medicaments #research #drugresearch #laboratories #labs #chemistry #chimie #biology #biologie #biopharma #rna #rnatherapeutics #arn #wewillwin

Happy to share the lab's new publication "Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag", appeared in @NatureBiotech couple of days ago. We describe BreakTag & BreakInspectoR, an NGS-based methodology along with the accompanied bioinformatics pipeline for the rapid, efficient & genome wide characterization of CRISPR nucleases activity and fidelity https://lnkd.in/dkKYWWkP

The first #CRISPR-based treatment was approved 2 weeks ago. But how many new gene editing technologies are waiting to be discovered? In this new Science study, Han Altae-Tran and colleagues at the Broad Institute of MIT and Harvard institute used a deep clustering algorithm to identify over 200 new CRISPR-associated systems from public sequencing data. Some of these systems may enable the development of improved DNA editing techniques for biomedical use. #genetherapy #bioinformatics

Novel Approaches for Correcting Gene Expression Insufficiency. A new molecular technology capable of binding to mRNA and regulating gene expression may offer a new avenue for treating diseases caused by haploinsufficiency, or the absence of one functional gene copy, according to a study published in Nature Communications. Read: https://lnkd.in/eXKE_kGd #sciencesdelavie #biotechnologie #biotechnology #innovation #recherche #sciences #medicaments #research #drugresearch #laboratories #labs #chemistry #chimie #biology #biologie #biopharma #rna #rnatherapeutics #arn #wewillwin

🧬 Breakthrough in CRISPR Technology: SEED/Harvest Method Unveiled! 🌟 Researchers at the Biozentrum, University of Basel, led by Prof. Markus Affolter, have developed a groundbreaking CRISPR-Cas9 method called SEED/Harvest. This innovative approach integrates the Single-Strand Annealing (SSA) repair pathway to enable precise and scarless DNA editing in fruit flies. 🦟🔬 The SEED/Harvest method allows for seamless genomic modifications, significantly enhancing our ability to study protein functions across various tissues and developmental stages. This technique combines two key steps: Marker Gene Insertion: Introduces a marker at the target site for isolating successful edits. Marker Excision and SSA Repair: Repairs DNA breakpoints seamlessly, leaving no unwanted scars. This advancement not only pushes the boundaries of genetic research but also opens up new avenues in biotechnology and medical research, including studying disease gene defects. The method promises more precise, efficient, and impactful research outcomes. 🚀 #CRISPR #Genetics #Biotechnology #MedicalResearch #Innovation #SEEDHarvest #UniversityOfBasel #ProteinResearch #GenomicEditing #ScientificBreakthrough