Monod Bio’s Post

AGO1 Protein

Introduction to Detergents for Membrane Protein Solubilisation One of the most difficult targets that we work with at Peak Proteins (A Sygnature Discovery) are membrane proteins, requiring additional steps and the use of detergents during their purification. In this blog post we discuss why detergents are used to purify membrane proteins, some of the different types of commonly used detergents, and how we use them. We have a dedicated membrane protein team led by Steven Harborne, experienced in producing membrane proteins in a variety of formats and structural determination. Learn more about our membrane protein capabilities - https://lnkd.in/eqCRwk55

💡 Eurofins DiscoverX is proud to introduce our new reagent Xtract-xDG kit: Your Solution for Membrane Protein Extraction! 🔬Our Xtract-xDG kit features a selection of glucoside detergents, widely used in biochemical and biophysical studies. With non-ionic amphiphilic composition, these micelle-producing detergents effectively disrupts lipid-protein interactions while preserving protein structure & function, offers great adaptability to membrane proteins of varying natures and in the most challenging chemical environments. Our solubilizing reagent kit contains mild detergents, designed with various specific alkyl chain lengths, allowing to efficiently screen for the optimal solubilizer.   Using our innovative detergents to take advantages from their properties: • Non-ionic amphiphilic molecules – Insensitive to ionic strength or pH changes  • Improved stability – Enhanced thermostability of extracted membrane proteins  • Detergent forming nanodisc-like structure – Native-like lipid environment for membrane protein studies 🚀 Whether you're exploring protein-protein interactions, approaching drug discovery studies, or unlock the complexities of cellular signaling pathways, our Xtract-xDG Kit drives innovation and advancement in your protein research. Learn more about our reagents for membrane protein extraction: https://lnkd.in/ezDbFNGz #EurofinsDiscoverX #EurofinsDiscovery #ProteinResearch #DrugDiscovery #ProteinReagents

🔬 Unleash the Power of Mutant Proteinase K! 🔬 Upgrade your research game with our cutting-edge Mutant Proteinase K - revolutionizing the world of protein digestion! 🚀 💡 Key Advantages: 🌟 Enhanced Specific Activity: Experience superior performance compared to wild-type Proteinase K. ❄️ Stability at Room Temperature: Our mutant variant stays robust, ensuring reliable results in various conditions. 🔍 Non-Specific Serine Protease: Boasting a broad substrate range for versatile applications. 🌡️ pH Tolerance (4-12): Active in a wide pH range, catering to diverse experimental setups. 🧪 Versatility: Ideal for digesting both native and denatured proteins in any experimental scenario. 🧬 Applications: From isolating mRNA and genomic DNA to modifying glycoproteins for structure studies. 🌡️ Temperature Resilience: Active between 15°C and 75°C, providing flexibility in experimental conditions. 💸 Affordable Excellence: Get all these benefits at an unbeatable price of only $40 per gram! Seize the opportunity to elevate your research with Mutant Proteinase K! 🚀 Don't miss out on this game-changing enzyme at an incredible price. 📧 Contact us now to place your order and transform your experiments! https://lnkd.in/d9Mky6tS #researchrevolution #enzymes #techadvancements #laboratory #biochemistry #innovationinscience

At PBL, we know #massspectrometry is awesome and #proteomics provides incredible #clinical potential. Need a refreshing read to go with your #pumpkin spice latte? Check out this great review (Steven Shuken) and better yet - come chat with our #experts about your next #targeted panel, #bioinformatics, or #discovery project! https://lnkd.in/gdtsiaZm

📢 More #NewProduct added!! 🌟🌟🌟 ReadiPrep™ Lipopolysaccharide (LPS) Isolation Kit provides a safe and effective way to isolate LPS from the outer membrane of Gram-negative bacteria. It utilizes a bacterial membrane lysis buffer and enzymatic protein digestion to extract pure LPS from bacterial cultures, which can be quantified using carbohydrate detection methods. Unlike conventional approaches that rely on hazardous organic chemicals like chloroform or phenol, this kit provides a safer and more time-efficient alternative for isolating high-purity LPS for research applications. Check it out!! https://lnkd.in/gkZtpJ8t #biotech #research #physiology

Here Shuford et al describes a cheaper, faster, simpler trypsin digestion for high-throughput targeted protein quantification! Which provided some very intresting results 🛎Overview: Multiple methodologies were evaluated to enhance tryptic digestion for the analysis of thyroglobulin, Factors assessed Included types of trypsin, microwave- and pressure-assisted systems, and enzyme concentration. 🎯Summary: Shuford et al conclusioned from the data, “The use of sequencing-grade trypsins and microwave/pressure-assisted systems did not offer significant advantages over the use of 'standard-grade' TPCK-treated trypsin in combination with a conventional incubator, once digestion time and additive had been optimized.” #massspectrometry #massspec #lcms #trypsin #chemistry #biotech #peptides #science #scienceandtechnology #sciencecommunication https://lnkd.in/eJ2pd3Zq

Starving cells hijack protein transport stations New proteins bound for outside the cell are manufactured on the endoplasmic reticulum (ER) – a snaking membrane inside the cell. Grape-like tubular outgrowths on the ER called ER exit sites serve as transport stations, collecting these newly synthesized proteins and delivering them to the next step in their journey. The team found that the stress triggers a series of molecules to work together to direct ER exit sites to lysosomes where they are destroyed – a novel pathway the cell may use to free up amino acids needed to make proteins inside the cell. First, the researchers showed how the ER exit sites are delivered to and ingested by certain types of lysosomes when cells are starved of nutrients. Next, the team detailed how this process happens. It starts when starving cells trigger the release of calcium from lysosomes. This causes an enzyme, ALG2, to get recruited to the ER exit sites where it binds to a structure called COPII that is attached to the neck that connects the ER to the ER exit site. This connection between ALG2 and COPII starts a process called ubiquitination, which is involved in protein degradation. A protein on the lysosome involved in bringing cellular material to the organelle for destruction recognizes the ubiquitin produced by the ubiquitination process, driving the ER exit site to the lysosome. Once at the lysosome, ALG2, which is attached to the ER exit site on one side, binds its other side to another protein, ALIX. ALIX interacts with ESCRT, a protein complex on the lysosome surface involved in ingestion. This interaction causes the ER exit site and the lysosome to become closer and closer until the ER exit site is engulfed and ingested by the lysosome. #ScienceMission #sciencenewshighlights https://lnkd.in/gznMTfBY

Protein L Conjugated to R-PE