NIH Clinical Center (CC)’s Post

𝗡𝗲𝘄 𝗽𝘂𝗯𝗹𝗶𝗰𝗮𝘁𝗶𝗼𝗻 𝗯𝗿𝗲𝗮𝗸𝘁𝗵𝗿𝗼𝘂𝗴𝗵 𝗳𝗼𝗿 𝗠𝗼𝗹𝗲𝗰𝘂𝗹𝗮𝗿 𝗠𝗼𝘂𝘀𝗲 𝗮𝗻𝗱 𝗦𝗲𝗽𝘀𝗶𝘀 𝗽𝗮𝗻𝗲𝗹! We are thrilled to share the latest publication on the Alifax molecular solution set to revolutionize the diagnosis and treatment of bloodstream infections, especially in critically ill patients. Key Highlights: 𝗥𝗮𝗽𝗶𝗱 𝗗𝗲𝘁𝗲𝗰𝘁𝗶𝗼𝗻: The Molecular Mouse system can identify pathogens and resistance genes from blood cultures within just an hour, significantly faster than traditional methods. 𝗛𝗶𝗴𝗵 𝗔𝗰𝗰𝘂𝗿𝗮𝗰𝘆: Demonstrated high accuracy in identifying bacteria and resistances, with consistent results compared to conventional culture methods and MALDI-TOF. 𝗖𝗹𝗶𝗻𝗶𝗰𝗮𝗹 𝗜𝗺𝗽𝗮𝗰𝘁: Influenced changes in antimicrobial therapy in over 61% of cases, ensuring timely and appropriate treatment adjustments. The study confirms that the Molecular Mouse system is a game-changer in the management of sepsis and other critical infections, helping to fight the growing threat of multidrug-resistant organisms. Read the full publication here: https://lnkd.in/d62g57dP #AntimicrobialResistance #InfectiousDiseases #Microbiology #HealthcareInnovation #ClinicalResearch #MolecularDiagnostics

The global rise of antimicrobial resistance has led to a renewed interest in phage therapy for treating bacterial infections. Isolating suitable phages for research is crucial, especially for combating Acinetobacter baumannii, known for its antibiotic resistance. A new lytic phage, Mystique, was discovered and characterized, showing a broad host range against A. baumannii strains. The study highlights how environmental conditions can significantly affect phage infectivity, impacting isolation and characterization efforts. https://buff.ly/4bQRJGM

We are delighted to announce the publication of our latest article in Critical Reviews in Microbiology, titled "Activation of the Lysosomal Damage Response and Selective Autophagy: The Coordinated Actions of Galectins, TRIM Proteins, and CGAS-STING1 in Providing Immunity Against Mycobacterium tuberculosis." This extensive study investigated the complex mechanisms by which Mycobacterium tuberculosis (Mtb) manipulates host immune responses and establishes virulence. Our research sheds light on the harmonized interaction between Mtb virulence factors and crucial host cellular components, providing insights into how the pathogen strategically regulates lysosomal damage response, selective autophagy, the CGAS-STING1 signaling pathway, type I interferon production, and inflammasome inhibition. Gaining a deeper understanding of Mtb evasion tactics is crucial for developing targeted interventions and advancing efforts to combat tuberculosis effectively. The following link can be used to access the full article. https://lnkd.in/dqAgNpdQ

Resistance genes have been identified of Enterobacter Cloacae and Enterobacter Aerogenes

For most healthy individuals, getting the flu is unpleasant but not life threatening. However, it lowers the host's immune system leaving them vulnerable to other viral or bacteria infections. UVic microbiologist Mariya Goncheva focuses her research on cases such as these. Currently, Goncheva is working to better understand the synergy seen between the Influenza A virus with the Staphylococcus aureus bacteria. Understanding the ways in which the bacteria affects the reproduction of the virus can be vital when constructing therapeutics. “The goal is for our research to provide new targets for the development of therapeutics and result in better patient outcomes—ultimately saving lives.” - Dr. Mariya Goncheva, biochemistry and microbiology professor and Tier 2 Canada Research Chair in Virology 🔗 Learn more about the research: https://ow.ly/4cT050SoMZs

#MDPIAwardWinners 📢 One of the winners of Pathogens 2024 Best Paper Award: "Methods of #Inactivation of Highly Pathogenic #Viruses for Molecular, Serology or Vaccine Development Purposes" 👨🎓 by Simon Elveborg, Vanessa M. Monteil, and Ali Mirazimi 👉 Free download available: https://lnkd.in/gCiQSYyY This article reviews available inactivation techniques published in peer-reviewed journals and their benefits and limitations in relation to the intended application. #SARSCoV2

https://lnkd.in/d5CicfpG IMPORTANCE The world needs entirely new forms of antibiotics as resistance to chemical antibiotics is a critical problem facing society. We addressed this need by developing a targeted enzyme therapy for a broad range of species and strains within mycobacteria and highly related genera including nontuberculous mycobacteria such as Mycobacteroides abscessus, Mycobacterium avium, Mycobacterium intracellulare, as well as Mycobacterium tuberculosis. One advantage of this approach is the ability to drive our lytic enzymes through encapsulation into macrophage-targeted liposomes resulting in attack of mycobacteria in the cells that harbor them where they hide from the adaptive immune system and grow. Furthermore, this approach shreds mycobacteria independent of cell physiology as the drug targets the mycobacterial envelope while sidestepping the host range limitations observed with phage therapy and resistance to chemical antibiotics. #savafa

#ACVC2024 Initial assessment of cardiogenic shock: from etiology and phenotyping to risk stratification tools. It is important to identify and phenotype your CS patients to get better outcomes.

#Cystitis battles and #AMR: Looking at both sides For most of my >40-years career as a doctor, the conventional wisdom was that #bacterial mutations occurred purely as random events. Nevertheless, AMR could occur, because the Law of Large Numbers indicates a multitude of rare events occur through replication in bacterial populations sufficient to cause infection. The “pure randomness” dogma was challenged in the last decade by people such as #SusanRosenberg in Texas and others whose research suggested bacteria under #antibiotic threat can actively target and accelerate their mutation capabilities. Nevertheless, today, some #microbiologists remain skeptical. Now research published in Front Microbiol. 2024;15:1373344, by Bergum et al https://lnkd.in/eFEknHa6 having studied #Ecoli responses to exposure to DNA-damaging ciprofloxacin, suggests that DNA damage provokes the bacteria into full-throttle damage repair activity, even at the expense of replication, but if that intense activity fails, they will mutate to adapt. Bacteria aren’t bowling pins passively hanging around to be smacked down. But repair and mutation still take time, although sometimes not much. Antibiotics must hit hard and fast to overwhelm the pathogens before they can adjust to the threat with biofilm secretion or AMR mutations. Protracted battles favour the bacteria. The oral route of antibiotic administration for treating cystitis seems far too slow to be reliably effective. The symptom relief produced may be mere palliation in many cases and likely accounts for the AMR that we see progressively more on our MSU test reports, because some bacteria survive the Rx assault.  AMR is rising due to great drugs being used more and more by us for the right reasons, but too inefficiently and, consequently, become ineffective. To my #antibioticsteward colleagues: if the right drug is used in the wrong way. Don’t blame “over-prescribing.” We need better treatments.