Investigating recombinant choline oxidase? - Check out this study where scientists successfully express and harness this enzyme's power! S. Lokesha, Y. S. Ravi Kumar, P. S. Sujan Ganapathy, Prashant Gaur, and H. M. Arjun isolated the gene from a soil bacterium, cloned it into an expression vector for overexpression in a Rosetta system. Next, they immobilized it onto Ni-Sepharose beads and optimized reaction conditions in a rotating bed reactor for choline to glycine betaine conversion. Access the study here: https://hubs.li/Q02dZ_6m0 #cholineoxidase #biocatalysis #betaine #enzymeimmobilization #enzyme
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⚡What was the lightning strikes, once-in-a-career idea that launched Ansa? A unique #DNAsynthesis technique that works with enzymes to create long, complex oligonucleotides. Ansa founders Daniel Lin-Arlow and Sebastian Palluk are featured in this dive into enzymatic DNA synthesis by The Scientist. Read on to learn more about how chemical approaches for synthesizing DNA oligonucleotides are being replaced by enzymatic methods, which can generate longer strands, such as gene fragments, with a lower error rate, without damaging DNA: https://lnkd.in/eKXbaE42 #SyntheticDNA #Ansamers #synbio #longDNA #biotech #DNAInnovation #complexDNA #customDNA #EnzymaticDNA
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Introducing the GDC BAM Slicing Download tool! Slice BAM files by variant, gene, position, or SNPs for individual cases in the GDC. Plus, download unmapped reads. #GDC #Bioinformatics :
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Did you know that there are various methods to create transgenic plants?🌱 To insert exogenous DNA into plant cells, there is not only Agrobacterium tumefaciens-mediated transformation (indirect method), as we show you in the last post, but also chemical-physical methods (direct methods). ✅Microinjection ✅Electroporation ✅Permeabilisation of protoplasts ✅ Gene gun ✅Agrobacterium tumefaciens Text by Martina Curci, PhD students Marie Skłodowska-Curie Actions
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Step by step, closing old chapters to make way for new and exciting beginnings! I am pleased to share a new publication related to my PhD. This challenging yet beautiful journey was made possible with the great support of Alberto Rodríguez Martín and Beatriz Galan Sicilia. From a circular economy and waste recovery perspective, we have unravelled the molecular mechanisms involved in producing hydrogen from 🅢🅨🅝🅖🅐🅢 using the photosynthetic bacterium 𝘙𝘩𝘰𝘥𝘰𝘴𝘱𝘪𝘳𝘪𝘭𝘭𝘶𝘮 𝘳𝘶𝘣𝘳𝘶𝘮. #genome #proteomics #evolution #metabolism
Boosting hydrogen production in Rhodospirillum rubrum by syngas-driven photoheterotrophic adaptive evolution
sciencedirect.com
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Associate Professor of Plant Biotechnology at National Institute of Laser Enhanced Sciences, Cairo University
I'm happy to share our most recent research paper, which describes a method for establishing a tomato regeneration and transformation system to target the gene responsible for ripening in tomatoes and produce a long-lived tomato by using CRISPR CAS/9 technique. However, more analysis is needed to confirm the effectiveness of our constructs by examining the transgenic plantlets using sequencing and other molecular techniques, which will soon be available, so stay tuned for the next
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New analysis type now available in Pluto! Gene/protein networks are valuable for visualizing how genes & proteins are predicted to be associated, whether through co-expression, literature evidence, or other relationships. In Pluto, you can create interactive networks from custom gene lists, DEG tables, and more. Customize network appearance and export in high resolution vector formats to showcase networks in your presentations and publications. See interactive networks for yourself with a live demo! https://lnkd.in/gHpz9r2X #bioinformatics #datavisualization #biotech #academia
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⚡️ 𝗚𝗲𝗻𝗦𝗰𝗿𝗶𝗽𝘁 𝗡𝗘𝗪 𝗙𝗟𝗔𝗦𝗛 𝗚𝗲𝗻𝗲 𝗦𝗲𝗿𝘃𝗶𝗰𝗲: 𝗧𝗵𝗲 𝗙𝗮𝘀𝘁𝗲𝘀𝘁 𝗮𝗻𝗱 𝗺𝗼𝘀𝘁 𝗔𝗳𝗳𝗼𝗿𝗱𝗮𝗯𝗹𝗲⚡️ 𝗘𝘅𝗽𝗲𝗿𝗶𝗲𝗻𝗰𝗲 𝘁𝗵𝗲 𝗙𝗟𝗔𝗦𝗛: 🚀𝗙𝗔𝗦𝗧𝗘𝗦𝗧 𝗧𝘂𝗿𝗻𝗮𝗿𝗼𝘂𝗻𝗱: Get your gene synthesis shipped in just 4 days. No ranges, no starting from – just a precise, reliable 4-day timeframe. 💰𝗙𝗟𝗔𝗧 𝗥𝗔𝗧𝗘 𝗣𝗿𝗶𝗰𝗶𝗻𝗴: Enjoy our flat rate $89 per construct. Affordable and predictable – just what researchers on a budget need. 🌐𝗣𝗿𝗼𝘂𝗱𝗹𝘆 𝗠𝗮𝗱𝗲 𝗶𝗻 𝘁𝗵𝗲 𝗨𝗦𝗔: *FLASH Gene services will be offered from our newly expanded facility in Piscataway, New Jersey and will also be available through our global production network 🔬𝗙𝗹𝗲𝘅𝗶𝗯𝗶𝗹𝗶𝘁𝘆 𝗮𝗻𝗱 𝗥𝗲𝗹𝗶𝗮𝗯𝗶𝗹𝗶𝘁𝘆: Add-on plasmid preparation service, adding just 2 extra business days, all with guaranteed plasmid yield and 100% sequence accuracy guaranteed. Order Now: https://ow.ly/JcjW50SkzMu #FLASHGene #Genesynthesis #Plasmidpreparation #Cloning #Price #flatrate
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Driving sustainable innovation @ ASPIDIA | Chief Scientific Officer and co-founder of ASPIDIA: researching sustainable and efficient clean water solutions to solve the emerging pollution crisis | #PFAS #microplastics
Uncovering PFAS Risks: Insights from Fathead Minnow Larvae Transcriptomics A recent study used a high-throughput transcriptomic assay to determine the concentration at which 20 PFASs elicit a gene expression response in fathead minnow larvae. This assay measures biological effects, although it is not directly linked to specific toxicity outcomes. The median concentration that elicited this transcriptomic response (referred to as the transcriptomic-based point of departure [tPOD]) was found to be 10 µM. Among the PFASs tested, longer-chain carboxylic acids were identified as some of the most potent, eliciting transcriptomic responses at concentrations below 1 µM. The results of the study showed that the observed tPODs were significantly higher than PFAS levels detected in tributaries of the North American Great Lakes, indicating a substantial margin of safety for humans. Therefore, there is no need to be alarmed about PFAS contamination when levels are very low and biologically ineffective, even for small organisms. However, it's important to reiterate that the international PFAS emergency calls for increased funding for research into new, sustainable and effective bioremediation methods, such as the ongoing project led by ASPIDIA. For those interested in learning more and supporting our efforts, please feel free to contact us via email. #pfas #foreverchemicals #bioremediation #enzymes #cleanwater #health https://lnkd.in/d6c4GxMk
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Plant Scientist 🇩🇪 Developing Products for Industry 💲 Commercial Breeding + Biotechnology | STEM Career Advising | Comics Artist
🌊 Scientists have uncovered a novel gene that indirectly enhances photosynthesis in cyanobacteria 🍀 🌊🍀 Proteins play crucial roles in regulating nitrogen assimilation in cyanobacteria, where nitrate is a primary nitrogen source. 🌊🍀 The protein NirP1, upregulated in low carbon conditions, binds to nitrite reductase (NiR), leading to nitrite accumulation and excretion. 🌊🍀 Excess nitrite inhibits photosynthesis. 🌊🍀 NirP1 likely competes with ferredoxin Fd1 for NiR binding, hindering nitrite reduction. 🌊🍀 NirP1 transcription is controlled by NtcA and an unknown factor, linking carbon and nitrogen assimilation. 🌊🍀 NirP1's action may prevent nitrite poisoning, possibly by storing it in the periplasm. 🌊🍀 This suggests a mechanism for cyanobacteria to regulate nitrite release based on physiological conditions. 🌊🍀 Despite the intuitive point of regulating nitrogen assimilation during nitrate uptake or reduction, NirP1's role highlights a different control point. 🌊🍀 Electron availability and potential benefits to coexisting microbes may influence nitrite excretion. 🌊🍀 While Prochlorococcus lacks NirP1 homologs, other cyanobacteria likely benefit from NirP1-mediated regulation in diverse environments. What do you think? 👍🏻 and 🔄 the knowledge :) Follow me for more ! Dr. Hamed Altaweel #cyanobacteria #bluegreenalgae #photosynthesis
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