Emma Raducanu lost in three sets against qualifier Lulu Sun of New Zealand to see her Wimbledon dream end in the fourth round.
The former US Open champion was the only British hopeful left in the women's draw after compatriots Harriet Dart and Sonay Kartal were beaten in round three. Her opponent in the fourth round, Sun, had eliminated eighth seed Zheng Qinwen - among others - after needing to come through qualifying just to make it into the main draw.
Raducanu beat ninth seed Maria Sakkari in round three after earlier victories over Renata Zarazua and Elise Mertens. A wrist problem had forced her to withdraw from the mixed doubles with Andy Murray, but she was fit to take to Centre Court on Sunday afternoon.
A slow start left Raducanu with it all to do against her opponent, with both players aiming to reach the quarter-finals at SW19 for the first time. After dropping the first set, the wild card battled back to claim the second but couldn't seal the deal, losing 2-6, 7-5, 2-6.
Sun took an early lead, breaking in the very first game, and was quickly 3-0 up in the first set. Raducanu tried to respond, only to surrender the opener by a 6-2 margin.
It was the first set the Brit had dropped all tournament, and all eyes were on how she would respond. The second set started better than the first, with Raducanu taking the first game, with both players trading blows and threatening - but not quite managing - to make a breakthrough.
Her 23-year-old opponent stood firm, but missed a golden chance to break early in the third when she sent a presentable opportunity long. Raducanu eventually took full advantage after surviving the scare, winning the second by seven games to five to force a decider, but her opponent had too much in the end.
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In the first game of the deciding set, Raducanu slipped while stretching for the ball. She was seen holding her leg as she received treatment. She returned to court, but was immediately broken by her opponent, with Sun then cementing that lead with a service hold and ultimately breaking for a second time before serving out for the match.
Sun only made her grand slam debut back in January at the Australian Open, and this was by far her best career performance even before she took to court on Sunday. She will now go up against Donna Vekic in the quarter-finals after the Croatian was involved in a rain-interrupted fourth round game against Paula Badosa, winning 6-2, 1-6, 6-4.
Earlier in the day, a cracker of a fourth round match saw Jasmine Paolini progress. Her opponent, Madison Keys, was forced to retire injured at 5-5 in the deciding set when it became clear she couldn't continue.
In the men's singles draw, the last of the British hopefuls fell in the third round. Cameron Norrie had beaten compatriot Jack Draper to set up a meeting with Alexander Zverev on Saturday, only for the fourth seed to claim a straight sets victory.