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Veterinary Parasitology
Veterinary Parasitology
PARASITE TAXONOMY
PARASITIC WORMS
Phylum-Platyhelminthes (flatworms,
flukes and tapeworms)
Phylum-Nemathelminthes (roundworms)
Phylum-Acanthocephala (thorny-headed
worms)
Phylum-Annelida (segmented worms,
night crawlers)
Phylum-Arthropoda (tongue worms)
Fasciola spp.
1. F. hepatica
2. F. gigantica
Scientific Classification
Kingdom: Animalia
Phylum: Platyhelminthes
Class: Trematoda
Subclass: Digenea
Order Echinostomida
Family: Fasciolidea
Genus: Fasciola
Species:hepatica &
gigantica
General Information
- Commonly known as liver fluke
- A parasitic flatworm
Geographical Distribution
- Found in Rural areas of
temperate and tropical
regions
- Especially located in regions
with cattle and sheep
herding
- Found on every continent
with nearly 180 million
people at risk and an
estimated 2.4 million
people already infected
worldwide.
Transmission
- Occurs through the ingestion of raw, fresh water
vegetation
- Plants become exposed to the metacercariae when
the body of water that the vegetation is growing in
becomes contaminated by eggs in the fecal mater of
the infested host
- A form of infection known as halzoun (in the Middle
East) is contracted by eating the raw liver of an
infected animal
Morphology
- Adult has a flat leaflike body
- About 20-30 mm long by 815 mm wide
- Has an anterior elongation
where oral and ventral
suckers are located
- Intestines are very branched
Hosts
- Cattle
- Sheep
- Goats
- Sometimes humans
Life Cycle
http://www.cdfound.to.it/hTML/fh2a.htm
Acute Phase
Cronic Phase
Halzoun
Ectopic Infection
Acute Phase
- Rarely seen in humans
- Fever, tender hepatomegaly, and abdominal
pain are frequent symptoms.
- Vomiting, diarrhea, and anemia may also be
present
Chronic Phase
- More common in human population
- Symptoms include: bilary cholic, abdominal pain,
tender hepatomegaly, and jaundice
- In children: severe anemia is common
- Inflammation of the bile ducts eventually leads to
fibrosis and a condition called pipestem liver
- Severe infections can lead to death
Diagnostic Tests
- Most widely used form of diagnosis is the directly observed
presence of F. hepatica eggs either in a stool sample,
duodenal aspirate or biliary aspirate
- Flukes do not begin to produce eggs until about 4 months
after infection, so you cannot test the stool
- Prior to 4 months: serological tests can be used
- FAST-ELISA (most popular)
Treatment
- Many countries use a 5-10 day course of oral
bithionol at 30mg/kg body weight
- Triclabendazole is a preferred antihelmintic agent,
but is unavailable in most countries.
- The resistance is rising to this drug
Control Methods
- Education
- Molluscicides: application of malluscicides to
decrease the population of Lymnaea snails
- Chemotherapy
Review Questions
-
Review (Contd)
- 3. What is the most effective way to treat fascioliasis?
a. bithionol
b. flagyl
c. triclabendazole
References
Sarah Richards and Max Karpyak
- Fascioliasis Retrieved: 2/19/2007 by Richards and
Karpyak
http://www.stanford.edu/class/humbio103/fascioli
asis/Fasciola.htm
- Fasciola hepatica. Wikipedia, free encyclopedia.
Retrieved: 2/19/2007
http://en.wikipedia.org/wiki/Fasciola_hepatica
- Fasciola hepatica. Retrieved: 2/19/2007
http://www.cdfound.to.it/html/fas1.htm
Trichostrongylus spp.
Morphology:
Zoonoses spp.
T. axei
T. orientalis
T. probolorus
T. skirjabini
T. colubriformis
Definitive host
Life cycle
Trich. spicules
Moniezia spp.
Introduction
Monieziosis is a cestodiasis caused by Moniezia sp. including
Moniezia expansa and M.benedni
Morphology
Moniezia spp. have unarmed scolices
with four large sucker and very wide segments with
bilateral genitalia.
scolices
M. expansa
M. benedeni
Characteristic of eggs
The egg of Moniezia spp. found
in cattle feces is
one of the few eggs that
appears square, and internally
the pear-shaped (pyriform
apparatus) characterristic of
anoplocephalid eggs can be
seen.
Pyriform apparatus
Epidemiology
The Monieziosis distribbution is world-wide.
Moniezia spp. are the commonest in young animals,
and are of low pathogenicity
Their incidence shows a seasonal fluctuation,
coinciding with the active period of the vectors.
Symptoms
When there are lots of Moniezia, the intestines will become
enteremphraxia even have been ruptured
The toxin of parasite cause young animals being neurosis,
including walking in circles spasm, non-food mastication.
clinical symptoms
Decreased appetite, emaciation, anaemia, listless, diarrhea,
gravid proglottid in feces
Lesions
The disease can make corpse become thin , the colour of
muscle turn light and make exudates in abdomen
increase.
Diagnosis
Diagnosis is based on a history of residence in
an endemic area and symptoms.
Fecal examination:
When there is no proglottids in feces ,using the
flotation method
Treatment
1.Bithionol: 50mg per kilogram of body weight for cattle,
or 75mg to 100mg for sheep, P.O.
2.Niclosamide:50mg per kilogram of body weight for
cattle, or 60mg to 75mg for sheep, P.O.
Prevention
1. Deworm before adult stage
2.Harmless disposal of feces
3.Improvement of breeding and management
ploughing and reseeding to avoid pasturing in the early
morning or at dust, when the peak of forage mite activities
regularly testing forage mite within the pasture
Introductory Recaps
Parasitism occurs in varying degrees:
1. parasitiasis- here the parasite is present in or
on the animal host, and is potentially
pathogenic but the animal does not show any
outward clinical signs of disease
Local pigs
Internal Parasites
The large roundworm (ascarid) is the most
common internal parasite of swine
Other roundworms include: stomach worms,
intestinal treadworms, kidney worms,
lungworms and nodular worms
Symptoms of worm infestation include: poor
growth, thin rough hair coat, diarrhea and
coughing
Endoparasites
Figure 6-48 Characteristic ovum of Ascaris suum, the swine ascarid or the large intestinal roundworm of pigs. The eggs are oval and
golden brown, with a thick, albuminous shell bearing prominent projections. They measure 70 to 89 m by 37 to 40 m.
(From Hendrix CM, Robinson E: Diagnostic parasitology for veterinary technicians, ed 3, St Louis, 2006, Mosby.)
PPP: 14 days
CS: piglets: enterocolitis
Dx: fecal flotation
Tx: piglets
sulfamethazine; sows decoquinate
PPP: 14 days
CS: piglets: 6 21 days, stunted, mortality
Dx: fecal flotation
Tx: piglets sulfamethazine; sows decoquinate
Oesophagostomum dentatum
nodular worm
PPP: 40 days
CS: asymptomatic, nodules
gut: enteritis
Dx: fecal flotation; Adults
LI
Condemnation intestine
Tx: Ivermectin, doramectin,
fenbendazole, levamisole,
pyrantel tartrate,
hygromycin, dichlorvos
CS: nonpathogenic
Dx: fecal sedimentation;
Adults stomach
Tx: Ivermectin,
doramectin, dichlorvos
Ascarops strongylina, egg. Courtesy of Dr.
Dietrich Barth, Merial
Figure 6-53 Egg of Stephanurus dentatus, the swine kidney worm. These eggs are strongyle type; that is, they are oval, thin-shelled
eggs containing 4 to 16 cells and measuring 90 to 120 m by 43 to 70 m. Eggs can be recovered from the urine by urine
sedimentation.
(From Hendrix CM, Robinson E: Diagnostic parasitology for veterinary technicians, ed 3, St Louis, 2006, Mosby.)
Balantidium coli
Protozoa
LC: 6 14 days
CS: mild severe enteritis
Dx: necopsy - LI ; fecal flotation
Tx: Tetracycline, metronidazole
ZOONOTIC
Figure 6-51 A, Balantidium coli of swine in histopathologic section. This photomicrograph was taken at low magnification. Note that
B. coli is quite large and easily visible (arrows). B, B. coli of swine in histopathologic section. This photomicrograph was taken at
higher magnification than A.
(From Hendrix CM, Robinson E: Diagnostic parasitology for veterinary technicians, ed 3, St Louis, 2006, Mosby.)
Figure 6-50 Trophozoite stage of Balantidium coli, the ciliated protozoan found in the large intestine of swine. The trophozoites
may be 150 by 120 m, with a sausage- to kidney-shaped macronucleus. They are covered with numerous rows of cilia and move
about the microscopic field with lively motility. The cyst is spherical to ovoid and 40 to 60 m in diameter, with a slight greenish-yellow
color.
(From Hendrix CM, Robinson E: Diagnostic parasitology for veterinary technicians, ed 3, St Louis, 2006, Mosby.)
Treatments
External Parasites
Lice (hog louse)
Are blood suckers. Approximately long
Result in economic loss due to reduced
performance
Control by use of insecticides
Insecticides available as a spray, pour-on,
dust, granule or injectible
Mange
Caused by microscopic mites that burrow
beneath the skin.
Causes severe itching
Will reduce swine performance
Control by the use of insecticides.
Ivermectin is the insecticide of choice
ECTOPARASITES
LC: 3 weeks
CS: pruritis and papules.
Dx: Skin scrapings
Tx: Ivermectin
ZOONOTIC
Figure 7-4 A, Thousands of nits can be cemented by female lice to the haircoat of domesticated animals. This calfs tail contains
thousands of nits. B, Pediculosis can be defined as infestation by either chewing or sucking lice, in this case, Haematopinus suis
infestation in a pig. C, Appearance of operculated nits viewed by compound microscope.
(From Hendrix CM, Robinson E: Diagnostic parasitology for veterinary technicians, ed 3, St Louis, 2006, Mosby.)
References
K Holtgrew-Bohling , Large Animal Clinical Procedures for
Veterinary Technicians, 2nd Edition, Mosby, 2012, ISBN:
97803223077323
Laboratory procedures for Veterinary Technicians, 5th edition,
2007, Hendrix C.M; Sirois M.
http://vetmed.iastate.edu/vdpam/new-vdpam-employees/foodsupply-veterinary-medicine/swine/swine-diseases
http://www.flockandherd.net.au/other/reader/sarcoptes%20pigs.
html
http://www.ent.iastate.edu/imagegallery/
http://nematode.net/NN3_frontpage.cgi?navbar_selection=specie
stable&subnav_selection=Ascaris_suum
References
http://www.ecvpath.org/october-2008/
http://cal.vet.upenn.edu/projects/dxendopar/parasitepages/hookl
ungstrongyloides/s_ransomi.html
http://parasitology.cvm.ncsu.edu/quiz/swine/exam2/swinequizEA.
php
http://uk.merial.com/producers/swine/woe/woe_04.asp
http://vetpda.ucdavis.edu/parasitolog/Parasite.cfm?ID=94
http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/2
2605.htm
http://www.wormbook.org/chapters/www_genomesTrichinella/g
enomesTrichinella.html
http://www.cesa.life.ku.dk/Cysticercosis.aspx
Insects
Ctenocephalides (Flea)
Infest dogs and cats
Vector for Dipylidium caninum, tularemia,
plague, etc.
Die at temperatures below 20 F for 48 hours
or at temperatures above 120 for several
days
Over 200 species of fleas worldwide
Severe infestations can cause anemia
Arachnids
Rare in cats
Causes areas of alopecia on head, neck, forelimbs.
Can be localized or generalized.
Diagnosed with skin scraping.
Is not contagious
Small numbers considered normal on skin flora
Genetic predisposition
Protozoa
Isopora (Coccidia)
Diarrhea in kittens and puppies.
May be fatal
One of most commonly diagnosed protozan
disorders in puppies and kittens.
Rarely a problem in mature animals
Very small in comparison to other parasites
Sarcocystis
Not very
pathogenic
in cats
Very
pathogenic
in sheep