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As 5013.9-2009 Food Microbiology Examination For Specific Organisms - Coliforms and Escherichia Coli by The T
As 5013.9-2009 Food Microbiology Examination For Specific Organisms - Coliforms and Escherichia Coli by The T
9—2009
®
Australian Standard
AS 5013.9—2009
Food microbiology
Method 9: Examination for specific organisms—
Coliforms and Escherichia coli by the triplicate
tube detection method
PREFACE
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This Standard was prepared by the Standards Australia Committee FT-024, Food Products
and Subcommittee FT-024-01, Food Microbiology (Constituted) to supersede, in part,
AS 1766.2.3—1992, Food microbiology, Method 2.3: Examination for specific organisms—
Coliforms and Escherichia coli.
The International Organization for Standardization (ISO) coliform and E. coli methods
adopted as Australian Standards contain a series of procedures for the enumeration of these
bacteria. There is no ISO qualitative procedure equivalent to the existing triplicate tube
method in AS 1766.2.3 and this procedure is retained as an Australian Standard for use in
food analyses.
The terms ‘normative’ and ‘informative’ have been used in this Standard to define the
application of the appendix to which they apply. A ‘normative’ appendix is an integral part
of a Standard, whereas an ‘informative’ appendix is only for information and guidance.
METHOD
1 SCOPE
This Standard sets out procedures for the examination of foods for coliforms and
Escherichia coli by the triplicate tube method. This is a qualitative test and is suitable for
determining the presence or absence of coliforms and E. coli in a stated quantity of material
under test.
NOTES:
1 A flow diagram of the procedures is shown in Appendix A.
2 The method describes a general method and modifications may be required according to the
material under test. Such modifications, if required, are prescribed in the method dealing with
particular products.
3 Where the test is to estimate the level of coliforms only as an index of potential spoilage of
the product and of hygiene, an incubation temperature of 30°C is specified. However, where
the test is to be extended to include the estimation or detection of E. coli, incubation at 37°C
is specified. Cultures which have been incubated initially at 30°C cannot be used for further
tests for E. coli.
4 Methods for the examination of rinse fluids and filterable liquid products for coliforms and
E. coli by membrane filtration are given in AS 5013.
5 Quantitative coliform and E. coli methods are given in AS 5013.3 and AS 5013.15
respectively.
© Standards Australia
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