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    As 5013.9-2009 Food Microbiology Examination for Specific Organisms - Coliforms and Escherichia Coli by the t

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    As 5013.9-2009 Food Microbiology Examination For Specific Organisms - Coliforms and Escherichia Coli by The T

    Uploaded by

    SAI Global - APAC
    Standards Preview PDFs from SAI Global InfoStore

    Copyright:

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    1 AS 5013.

    9—2009
    ®
    Australian Standard
    AS 5013.9—2009

    Food microbiology
    Method 9: Examination for specific organisms—
    Coliforms and Escherichia coli by the triplicate
    tube detection method

    PREFACE
    This is a free 1 page sample. Access the full version at http://infostore.saiglobal.com.

    This Standard was prepared by the Standards Australia Committee FT-024, Food Products
    and Subcommittee FT-024-01, Food Microbiology (Constituted) to supersede, in part,
    AS 1766.2.3—1992, Food microbiology, Method 2.3: Examination for specific organisms—
    Coliforms and Escherichia coli.
    The International Organization for Standardization (ISO) coliform and E. coli methods
    adopted as Australian Standards contain a series of procedures for the enumeration of these
    bacteria. There is no ISO qualitative procedure equivalent to the existing triplicate tube
    method in AS 1766.2.3 and this procedure is retained as an Australian Standard for use in
    food analyses.
    The terms ‘normative’ and ‘informative’ have been used in this Standard to define the
    application of the appendix to which they apply. A ‘normative’ appendix is an integral part
    of a Standard, whereas an ‘informative’ appendix is only for information and guidance.

    METHOD

    1 SCOPE
    This Standard sets out procedures for the examination of foods for coliforms and
    Escherichia coli by the triplicate tube method. This is a qualitative test and is suitable for
    determining the presence or absence of coliforms and E. coli in a stated quantity of material
    under test.
    NOTES:
    1 A flow diagram of the procedures is shown in Appendix A.
    2 The method describes a general method and modifications may be required according to the
    material under test. Such modifications, if required, are prescribed in the method dealing with
    particular products.
    3 Where the test is to estimate the level of coliforms only as an index of potential spoilage of
    the product and of hygiene, an incubation temperature of 30°C is specified. However, where
    the test is to be extended to include the estimation or detection of E. coli, incubation at 37°C
    is specified. Cultures which have been incubated initially at 30°C cannot be used for further
    tests for E. coli.
    4 Methods for the examination of rinse fluids and filterable liquid products for coliforms and
    E. coli by membrane filtration are given in AS 5013.
    5 Quantitative coliform and E. coli methods are given in AS 5013.3 and AS 5013.15
    respectively.

    © Standards Australia
    This is a free preview. Purchase the entire publication at the link below:

    AS 5013.9-2009, Food microbiology Examination


    for specific organisms - Coliforms and Escherichia
    coli by the triplicate tube detection method
    This is a free 1 page sample. Access the full version at http://infostore.saiglobal.com.

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