SAFC Biosciences Scientific Posters - Cell Xpress™ Cell Line Optimization Service Using Laser-Enabled Analysis and Processing (LEAP™) Technology
SAFC Biosciences Scientific Posters - Cell Xpress™ Cell Line Optimization Service Using Laser-Enabled Analysis and Processing (LEAP™) Technology
Angela M. Davis, Genova A. Richardson, Nan Lin, Jennifer R. Cresswell, Matthew V. Caple and Kevin J. Kayser
Cell Sciences and Development, SAFC Biosciences 2909 Laclede Avenue, Saint Louis, MO 63103, USA
Introduction to Cell Xpress Schematic Illustration: Cell XpressCapture and Detection Applications
SAFC Biosciences (SAFCB) Cell Xpress technology combines in situ imaging with laser
Laser Enrichment of A Transfected “Pool” Population C
manipulation to identify, select, and monitor expansion of high recombinant protein
secreting clones. Cell Xpress provides significant advantages over microscope-based A
platforms with respect to throughput, robustness, and automation. Cell Xpress
Average Intensity
SAFCB medium, feed, and process optimization, can result in a significant increase
in productivity from cell culture-based processes. SAFCB uses this integrated process
development platform to develop media and feed for use in the production of a cell
culture-derived therapeutic protein from our customers. SAFCB employs Cell Xpress
to analyze secretion heterogeneity of an initial cell population which may be either
a clonal cell line or a transfected pool. The initial population is laser processed to Normalized Secretion
enrich the population for high producing cells by eliminating low or non-producing
cells in the population. Following two to three rounds of enrichment, single cell
clones are generated from the enriched pool. Clones and enriched populations are
then expanded and screened for growth and productivity performance.
Transfected Pool Cell Xpress Enriched
Transfected Pool
The LEAP Instrument
In situ detection of secreted recombinant IgG: Fluorescent-labeled detection reagent detects
The LEAP Instrument and the Proprietary C-lect™ 384-Well Plate secreted rIgG (red); live cells are stained with viable cell stain (green).
producers
The Benefits of Cell Xpress
Intensity (SAAI)
Acknowledgements
Correlation of Maximum Volumetric Productivity vs. Normalized Secretion Area Average Intensity
Erika Holroyd, Kathy Roeder
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