Roto Fix 32 Service Manual
Roto Fix 32 Service Manual
KG AB1206DEENFRIT
ROTOFIX 32 A
DE
Bedienungsanleitung...................................................... 5
EN
Operating Instructions.................................................... 18
FR
Mode d'emploi ................................................................. 30
IT
Istruzioni per l'uso .......................................................... 43
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A
Fig. 1
RCF
START
STOP
IMPULS
Fig. 2 ROTOFIX 32 A
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Andreas Hettich GmbH & Co. KG
Fhrenstrae 12, D-78532 Tuttlingen / Germany
Phone +49 (0)7461 / 705-0
Fax +49 (0)7461 / 705-125
[email protected], [email protected]
www.hettichlab.com
2006 by Andreas Hettich GmbH & Co. KG
All rights reserved. No part of this publication may be reproduced without the written prior permission of the copyright
owner.
nderungen vorbehalten! , Modifications reserved! , Sous rserve de modifications ! , Con riserva di modifiche!
AB1206DEENFRIT / 09.07
EN
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1 Use according to specification
The machine presented here is a medical product (laboratory centrifuge) according to the IVD guideline 98/79/EG.
The centrifuge is used to separate substances or substance mixtures with a density of max. 1.2 kg/dm. This also
includes substances and substance mixtures of human origin. The centrifuge is only intended to be used for this
purpose. A different use or application over and above this is deemed not in accordance with the specifications. The
company Andreas Hettich GmbH & Co. KG undertakes no liability for damages resulting therefrom.
Belonging to the application according to specification is also the observance of all references contained in the
Instruction Manual and compliance with the inspection and maintenance works.
2 Residual risks
The machine is constructed according to the state of the art and the recognized technical safety regulations.
Improper use and handling can result in dangers to life and limb of the user or third parties and impairments to the
machine or to other material assets. The machine is only to be used for the specified applications and only in an
impeccable technical safety condition.
Disturbances that can interfere with the safety are to be immediately rectified.
3 Technical specifications
Manufacturer
Andreas Hettich GmbH & Co. KG
D-78532 Tuttlingen
Model ROTOFIX 32 A
Type 1206 1206-01 1206-02
Mains voltage ( 10%) 208 240 V 1 100 127 V 1
Mains frequency 50 60 Hz 50 60 Hz
Connected load 300 VA 300 VA
Current consumption 1.4 A 3.0 A
Max. capacity 4 x 100 ml / 32 x 15 ml
Allowed density
1.2 kg/dm
3
Speed (RPM) 6000
Force (RCF) 4186
Kinetic energy 3160 Nm
Obligatory inspection (BGR 261) no
Ambient conditions (EN 61010-1)
Set-up site Indoors only
Altitude Up to 2000 m above sea level
Ambient temperature 2C to 40C
Humidity Maximum relative humidity 80% for temperatures up to 31C, linearly
decreasing to 50% relative humidity at 40C.
Excess-voltage category
(IEC 60364-4-443)
Pollution degree 2
Device protection class
Not suitable for use in explosion-endangered areas.
EMC
Emitted interference (suppression
of radio interference)
EN 55011,
Group 1, Class B
EN 61000-3-2
EN 61000-3-3
FCC Class B
Interference immunity EN 61000-6-2 ----
Noise level (dependent on rotor) 57 dB(A)
Dimensions
Width 366 mm
Depth 430 mm
Height 257 mm
Weight 23 kg approx. 27 kg
EN
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4 Notes on safety
No claim under guarantee will be considered by the manufacturer unless the above instructions have
been adhered to.
Before the initial operation of your centrifuge you should read and pay attention to the operating
instructions.
Only personnel that has read and understood the operating instructions are allowed to operate the
device.
Along with the operating instructions and the legal regulations on accident prevention, you should also follow the
recognised professional regulations for working in a safe and professional manner.
These operating instructions should be read in conjunction with any other instructions concerning accident
prevention and environmental protection based on the national regulations of the country where the device is to
be used.
This centrifuge is a state-of-the-art piece of equipment which is extremely safe to operate.
However, it can lead to danger for users or others if used by untrained staff, in an inappropriate way or for a
purpose other than that it was designed for.
The centrifuge should be installed on a good, stable base.
Before using the centrifuge absolutely check the rotor for firm placement.
When the centrifuge is running, according to IEC 61010-2-020, no persons, dangerous substances or objects may
be within the safety margin of 300 mm around the centrifuge.
The centrifuge must not be moved or knocked during operation.
In case of fault or emergency release, never touch the rotor before it has stopped turning.
To avoid damage due to condensate, when changing from a cold to a warm room the centrifuge must either heat
up for at least 3 hours in the warm room before being connected to the mains, or run hot for 30 minutes in the
cold room.
Only the rotors and accessories approved by the manufacturer for this device may be used (see chapter
"Anhang/Appendix, Rotoren und Zubehr/Rotors and accessories").
The centrifuge rotor may only be loaded in accordance with the chapter "Loading the rotor".
When centrifuging with maxim revolutions per minute the density of the materials or the material mixtures may not
exceed 1.2 kg/dm
3
.
The centrifuge may only be operated when the balance is within the bounds of acceptability.
The centrifuge may not be operated in explosion-endangered areas.
The centrifuge must not be used with:
inflammable or explosive materials
materials that react with one another producing a lot of energy.
If users have to centrifuge hazardous materials or compounds contaminated with toxic, radioactive or pathogenic
micro-organisms, they must take appropriate measures.
For hazardous substances centrifuge containers with special screw caps must strictly be used. In addition to the
screw cap centrifuge containers, for materials in hazard category 3 and 4 a biosafety system must be used (see
the World Health Organisations Laboratory Biosafety Manual).
Under a biosafety system small drips and aerosols are prevented from escaping by a bioseal (packing ring)
located between the hanger and the lid.
If the hanger of a biosafety system is used without the lid, the packing ring must be removed from the hanger in
order to prevent the packing ring from being damaged during the centrifugation run. Damaged packing rings must
not be used to seal the biosafety system.
Without the use of a biosafety system the centrifuge is not microbiologically sealed in the sense of the EN
610101-2-020 standard.
For further details of available biosafety systems see chapter "Anhang/Appendix, Rotoren und Zubehr/Rotors
and accessories". If in doubt, you should obtain relevant information from the manufacturer.
The centrifuge must not be operated with highly corrosive substances which could impair the mechanical integrity
of rotors, hangers and accessories.
Rotors, suspensions and accessories that possess traces of corrosion or mechanical damage or if their term of
use has expired may not be used any longer.
Repairs must only be carried out by personnel authorised to do so by the manufacturer.
Only original spare parts and original accessories licensed by the Andreas Hettich GmbH & Co. KG company are
allowed to be utilised.
The following safety regulations apply:
IEC 61010-1 and IEC 61010-2-020 as well as their national deviations.
The safe operation and reliability of the centrifuge can only be guaranteed if:
the centrifuge is operated in accordance with the operating instructions,
the electrical installation on the site where the centrifuge is installed conforms to the demands of IEC
stipulations,
prescribed tests to BGV A1, BGR 261 are carried out by an expert.
EN
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5 Symbol meanings
Symbol on the machine:
Attention, general hazard area.
Before using the centrifuge implicitly read the operating instructions and pay attention to the safety
relevant references!
Symbol in the operating instructions:
Attention, general hazard area.
This symbol refers to safety relevant warnings and indicates possibly dangerous situations.
The non-adherence to these warnings can lead to material damage and injury to personal.
Symbol in the operating instructions:
This symbol refers to important circumstances.
Symbol on the machine and in the operating instructions:
Symbol for the separate collection of electric and electronic devices according to the guideline
2002/96/EG (WEEE). The device belongs to Group 8 (medical devices).
Applies in the countries of the European Union, as well as in Norway and Switzerland.
6 Deli very checklist
The following items and accessories are delivered with the centrifuge:
1 Connecting cable
2 Fuses (type 1206-02: 3 fuses)
1 Lubricating grease for trunnions
1 Hex. pin driver
1 Release pin
1 Notes on moving the equipment safely
1 Operating instructions
The rotor(s) and associated accessories are included in the delivery in the quantity.
7 Unpacking the centrifuge
Lift the carton upward and remove the padding.
Do not lift by the handle rail.
Observe the weight of the centrifuge, refer to chapter "Technical specifications".
Lift the centrifuge on both sides with an appropriate number of helpers and place it on the laboratory table.
8 Initial operation
According to the laboratory instrument standards IEC 61010-2-020 an emergency switch to separate power
supply in the event of a failure must be installed in the building electrical system.
This switch has to be placed remote from the centrifuge, prefered outside of the room in which the centrifuge is
installed or near by the exit of this room.
Remove the transportation safety device from the bottom of the housing, see sheet Transportation safety
device".
Position the centrifuge in a stable and level manner in a suitable place. During set-up, the required safety margin
of 300 mm around the centrifuge is to be kept according to IEC 61010-2-020.
When the centrifuge is running, according to IEC 61010-2-020, no persons, dangerous substances or
objects may be within the safety margin of 300 mm around the centrifuge.
Do not place any object in front of the ventiduct.
Keep a ventilation area of 300 mm around the ventiduct.
Check whether the mains voltage tallies with the statement on the type plate.
Connect the centrifuge with the connection cable to a standard mains socket. For connection ratings refer to
Chapter "Technical specifications".
Turn on the mains switch. Switch position "".
The last used centrifuge data will be displayed.
Open the lid.
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9 Opening and closing the lid
9.1 Opening the lid
The lid can only be opened when the centrifuge is switched on and the rotor is at rest. If it cannot be
opened under these circumstances, see the section on Emergency release.
Swing handle rail on the lid upwards. The symbol " " (lid open) illuminates in the rotation indicator .
Open the lid.
9.2 Closing the lid
Do not bang the lid shut.
Place the lid and swing handle rail on the lid downward. The symbol " " (lid closed) illuminates in the rotation
indicator .
10 Installation and removal of the rotor
A
B
C
D
Clean the motor shaft (C) and the rotor drilling (A), and lightly grease the motor shaft
afterwards. Dirt particles between the motor shaft and the rotor hinder a perfect seating
of the rotor and cause an irregular operation.
Place the rotor vertically on the motor shaft. The motor shaft dog (D) has to fit in the
rotor slot (B). The alignment of the groove is labelled on the rotor.
Tighten the rotor tension nut with the supplied wrench by turning in a clockwise
direction.
Check the rotor for firm seating.
Loosening the rotor: Loosen the tension nut by turning in a counter clockwise direction,
and turning until the working point for lifting. After passing the working point for lifting
the rotor is loosened from the motor shaft cone. Turn the tension nut until the rotor is
able to be lifted from the motor shaft.
11 Loading the rotor
Standard centrifuge containers of glass will not stand RCF values exceeding 4000 (DIN 58970, pg. 2).
Check the rotor for firm seating.
With swing-out rotors all rotor positions must be lined with identical hangers. Certain hangers are marked with
the number of the rotor position. These hangers may only be used in the respective rotor position.
The rotors and hangers may only be loaded symmetrically. For authorised combinations see Chapter
"Anhang/Appendix, Rotoren und Zubehr/Rotors and accessories".
In the case of angle rotors all possible rotor positions must be loaded, see chapter "Anhang/Appendix, Rotoren
und Zubehr/Rotors and accessories".
On certain suspensions, the weight of the maximum load and the maximum weight of the suspension when it is
fully equipped is displayed. This weight may not be exceeded. The weight specified for the maximum loading
includes the total weight of adapter, frame, centrifuging container and content.
In containers with rubber inserts, the same number of rubber inserts must always be among the centrifuge
containers.
Always fill the centrifuge containers outside of the centrifuge.
No liquid should be allowed to enter the centrifugal chamber during filling and swinging out of the hangers.
The maximum filling quantity for the centrifuge containers specified by the manufacturer must not be exceeded.
In order to maintain the weight differences within the centrifuge container as marginal as possible, a consistent
fill level in the containers is to be heeded.
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12 Control and display elements
See figure on page 2.
Fig. 2: Display and control panel
12.1 Symbols on the control panel
Rotation indicator. The rotation indicator lights up and rotates anticlockwise while the rotor is turning.
When the rotor is stationary, the status of the lid is displayed by symbols in the rotation indicator:
Symbol : Lid open
Symbol : Lid closed
Operator errors and occurring faults are indicated on the display (see Chapter "Faults").
12.2 Keys and setting options
RPM/RCF x 100
Speed
A numeric value of 500 RPM up to the maximum rotor speed can be set. For maximum rotor speed,
see chapter "Anhang/Appendix, Rotoren und Zubehr/Rotors and accessories". Preset in steps of
100 (RPM =displayed value x 100).
If the key or is kept pressed, the value changes with increasing speed.
Display the brake step and the centrifuging radius.
t
Running time
- Preset from 1 - 99 minutes, in 1 minute steps
- Continuous operation "--"
Centrifuging radius. Input in centimeters. Preset from 5 - 16 centimeters, in 1 centimeter steps. For
centrifuging radius, see chapter "Anhang/Appendix, Rotoren und Zubehr/Rotors and accessories".
Braking steps 0 or 1. Step 1 =short run-down time, Step 0 =long run-down time.
If the key or is kept pressed, the value changes with increasing speed.
START
Start centrifugation run.
STOP
End centrifugation run.
The rotor runs down with the preselected brake step.
Save the brake step and the centrifuging radius.
RCF
Display of the relative centrifugal force (RCF).
The display of the relative centrifugal force (RCF) appears while the key
RCF
is kept pressed.
IMPULS
Short-time centrifugation.
The centrifugation run occurs while the key
IMPULS
is kept pressed.
Display the brake step and the centrifuging radius.
EN
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13 Setting the brake step
Switch off the mains switch.
Keep the key beneath the speed indicator and the key
IMPULS
pressed simultaneously.
Switch on the mains switch and release the keys again.
Press the key beneath the speed indicator until the following display appears:
RCF
START
STOP
IMPULS
RCF
START
STOP
IMPULS
The machine version set in the factory (e.g. 7) is displayed in the speed indicator and the set brake step is
displayed in the time indicator.
Set the desired brake step with the keys beneath the time indicator.
Step 1 =short run-down time, Step 0 =long run-down time.
For run-down times, see chapter "Anhang/Appendix, Rotoren und Zubehr/Rotors and accessories".
Press the key
STOP
to save the setting.
14 Setting the centrifuging radius
The centrifuging radius must be entered in centimeters.
Switch off the mains switch.
Keep the key beneath the speed indicator and the key
IMPULS
pressed simultaneously.
Switch on the mains switch and release the keys again.
Press the key beneath the speed indicator until the following display appears:
RCF
START
STOP
IMPULS
The set centrifuging radius is displayed in the speed indicator.
Set the desired centrifuging radius with the keys beneath the time indicator.
For centrifuging radius, see chapter "Anhang/Appendix, Rotoren und Zubehr/Rotors and accessories".
Press the key
STOP
to save the setting.
15 Centrifugation
When the centrifuge is running, according to IEC 61010-2-020, no persons, dangerous substances or objects
may be within the safety margin of 300 mm around the centrifuge.
If the permissible weight difference is exceeded within the rotor loading, the drive switches off during the run-
up time, and error -3- is displayed (see chapter "Faults").
The centrifugation run can be interrupted at any time by pressing the key
STOP
.
The time and speed can be changed during the centrifugation run, with the keys .
If the key or is kept pressed, the value changes with increasing speed.
After a centrifugation run, the display flashes until the cover is opened or a key is pressed.
If the symbol " " (lid closed) and " " (lid open) flashes alternately in the rotation indicator , operation of the
centrifuge can only be continued after opening the lid.
If rot xx is displayed, no centrifugation run has taken place because the rotor has been changed, see chapter
"Rotor Identification".
Switch on the mains switch (switch position "").
Load the rotor and close the centrifuge cover.
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15.1 Centrifugation with preselected time
Set the desired speed with the keys beneath the speed indicator.
Set the desired time with the keys beneath the time indicator.
Press the key
START
. The rotation indicator appears while the rotor is turning.
The time is displayed in minutes. The last minute is counted down in seconds.
When the time is displayed in minutes, a point flashes next to the number.
After expiry of the time or if the centrifugation run is interrupted by pressing the key
STOP
, the rotor runs down
with the set brake step.
During the centrifugation run, the rotor speed or the resulting RCF value and the remaining time are displayed.
15.2 Continuous operation
Set the desired speed with the keys beneath the speed indicator.
Set the time to zero with the key beneath the time indicator. "--" is displayed.
Press the key
START
. The rotation indicator appears while the rotor is turning. The time count starts from 0.
The first minute is counted up in seconds, and then the time is displayed in minutes.
When the time is displayed in minutes, a point flashes next to the number.
Press the key
STOP
to end the centrifugation run. The rotor runs down with the set brake step.
During the centrifugation run, the rotor speed or the resulting RCF value and the expired time are displayed.
15.3 Short-time centrifugation
Set the desired speed with the keys beneath the speed indicator.
Keep the key
IMPULS
pressed. The rotation indicator appears while the rotor is turning. The time count starts
from 0.
The first minute is counted up in seconds, and then the time is displayed in minutes.
When the time is displayed in minutes, a point flashes next to the number.
Release the key
IMPULS
again to end the centrifugation run. The rotor runs down with the set brake step.
During the centrifugation run, the rotor speed and the expired time are displayed.
15.4 Display of the relati ve centrifugal force (RCF)
The relative centrifugal force (RCF) can be displayed during the centrifugation run.
If the relative centrifugal force (RCF) is used, the centrifuging radius must be entered.
Keep the key
RCF
pressed during the centrifugation run.
The relative centrifugal force (RCF) appears in the speed indicator (RCF =displayed value x 100).
Release the key
RCF
again. The speed is displayed.
16 Relati ve centrifugal force (RCF)
The relative centrifugal force (RCF) is given as a multiple of the acceleration of gravity (g). It is a unit-free value and
serves to compare the separation and sedimentation performance.
These values are calculated using the formula below:
1,118 r
2
1000
RPM
RCF =
1000
1,118 r
RCF
RPM
=
RCF =relative centrifugal force
RPM =rotational speed (revolutions per minute)
r =centrifugal radius in mm = distance from the centre of the turning axis to the bottom of the centrifuge.
For more on the centrifugal radius see the chapter Anhang/Appendix,
Rotoren und Zubehr/Rotors and accessories.
The relative centrifugal force (RCF) stands in relation to the revolutions per minute and the centrifugal
radius.
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17 Centrifugation of materials with higher density
The rotors are designed to centrifuge substances up to a maximum mean homogenous density of 1.2 kg/dm
3
when
rotating at the stated speed.
Denser substances must be centrifuged at lower speed.
The permissible speed can be calculated using the following formula:
speed Rated x
density Greater
1.2
= ) (n speed Reduced red
e.g.: RPM 4000, density 1.6 kg/dm
3
RPM 3464 = 4000 x
1.6
1.2
= nred
If in doubt you should obtain clarification from the manufacturer.
18 Rotor Identification
After every start of a centrifugation run the rotor utilised is identified.
After a rotor change, the drive switches off and the rotor code (rot xx) is displayed.
Press the key
START
. The last used centrifuge data will be displayed..
A further operation of the centrifuge is only possible after a single opening of the lid.
If, following a rotor change, the maximum speed of the rotor is less than the set speed, the speed is limited to
the maximum speed of the rotor.
19 Emergency release
The lid cannot be opened during power failure. An emergency release has to be executed by hand.
For emergency release disconnect the centrifuge from the mains.
Open the lid only during rotor standstill.
Only the plastic release pin provided may be used for emergency release.
See figure on page 2.
Switch off the mains switch (switch position "0").
Look through the window in the lid to be sure that the rotor has come to a standstill.
Insert the release pin (see scope of supply) horizontally into the hole (Fig. 1, A). Push the unlocking pin in until
the handle can be lifted when the pin is pressed down.
Open the lid.
20 Maintenance and servicing
Pull the mains plug before cleaning.
Before any other cleaning or decontamination process other than that recommended by the manufacturer is
applied, the user has to check with the manufacturer that the planned process does not damage the device.
Cleaning agents and disinfectants which lie in the pH range 5 8 are to be utilised. Alkaline cleaning agents with
a pH value >8 are to be avoided.
In order to prevent appearances of corrosion through cleaning agents or disinfectants, the application guide from
the manufacturer of the cleaning agent or disinfectant are absolutely to be heeded.
20.1 Centrifuge
Clean the centrifuge housing and the centrifuging chamber regularly, using soap or a mild detergent and a damp
cloth if required. For one thing, this services purposes of hygiene, and it also prevents corrosion through
adhering impurities.
In the event of condensation water formation, dry the centrifugal chamber by wiping out with an absorbent cloth.
If infectious materials penetrates into the centrifugal chamber this is to be disinfected immediately. For surface
disinfection we recommend Bacillolmanufactured by Bode Chemie in Hamburg or Biocidal ZF
TM
from the
company WAK-Chemie Medical GmbH in Steinbach.
Lightly grease the rubber seal of the centrifugal chamber after every cleaning.
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20.2 Rotors and Attachments
In order to prevent corrosion and material changes, rotors and accessories must be cleaned regularly with soap
or a mild detergent and a damp cloth. Cleaning is recommended at least once a week, even better after every
usage.
If the rotor or accessory parts are contaminated by pathogenic or radioactive material, a suitable cleaning has to
be executed. For disinfection we recommend Helipur