Mini Prep Cell and Model 491 Prep Cell Starter Kit Instructions
Mini Prep Cell and Model 491 Prep Cell Starter Kit Instructions
This is your Prep Cell starter kit. The kit has been provided to demonstrate how well the Prep Cell resolves two
adjacent proteins and familiarize you with the assembly and operation of the Prep Cell. The sample in the starter kit
consists of two prestained proteins, Soybean Trypsin Inhibitor (apparent MW 27,500 +/- 10%) and Carbonic
Anhydrase (apparent MW 32,500 +/- 10%). Migration of these two blue proteins will demonstrate how straight these
bands will migrate during the run. This run can be monitored. It is important to note that as a consequence of the
staining process, the bandwidths of the prestained proteins are greater than those of unstained proteins.
Gel composition: 10% T/2.67% C - Resolving gel
4% T/2.67% C - Stacking gel
Gel length: Mini Prep Cell Model 491 Prep Cell
Resolving gel: 5.5 cm (2 ml) 5.5 cm (20 ml)
Stacking gel: 0.5 cm (200 µl) 0.5 cm (2 ml)
Gel tube size: 7 mm ID 28 mm ID
Sample load: 30 µl 300 µl (~150 µg of each protein)
All reagents necessary for three trial runs are included in this kit. It is advisable to perform at least one trial run to
familiarize yourself with assembly and operation of the Prep Cell. Detailed diagrams and instructions are provided in
the Prep Cell instruction manual. Please read the manual before your first run.
Product Information
Catalog
Number Description
161-5104 Mini and Model 491 Prep Cell Starter Kit with Standard
161-5101 Mini and Model 491 Prep Cell Starter Kit without Standard
161-0323 Mini and Model 491 Prep Cell Standard
Bio-Rad
Laboratories
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LIT466 Rev D