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Retention Behavior of Carboxylic Acids On Highly Cross-Linked Poly (Styrene-Divinylbenzene) - Based and Silica-Based Cation Exchangers
Retention Behavior of Carboxylic Acids On Highly Cross-Linked Poly (Styrene-Divinylbenzene) - Based and Silica-Based Cation Exchangers
CHROMATOGRAPHYA
ELSEVIER
C h r i s t i a n W. K l a m p f l ' , W o l f g a n g B u c h b e r g e r ,
Guenter Rleder,
G u e n t h e r K. B o n n b
Abstract
A highly cross-linked porous poly(styrene-divinylbenzene) resin was reacted with sulfuric acid to give strong cationexchange resins with capacities from 0.2 to 1.9 mequiv./g. The retention behavior of mono- and dicarboxylic acids on these
resins was compared with results obtained from silica-based cation exchangers functionalized with alkylsulfonic acid or
phenylsulfonic acid groups. The contribution of different retention mechanisms like ion exclusion, hydrophobic interaction
and adsorption to the separation of the analytes on these chromatographic supports was discussed. Additionally the
applicability of silica-based cation exchangers was tested in the field of silage analysis.
1. Introduction
24
2. Experimental
2.1. Instrumentation
Table 1
Reaction conditions for resin sulfonation
Capacity (mequiv./g)
0.0
0.2
0.6
1.2
1.9
H2SO 4 (ml)
Temperature (C)
30
15
15
15
30
50
50
50
4
60
90
120
0
0
75
80
25
- - x - - oxMc ec~
1
iIg
-X
26
--x-- forrdcacid
acellcacid
3.5.
3.0.
vab~ alcd
2.5,
_
'" -'- - - - , ~ , ~ . ~
'
i 2.0'
1.5.
1.0"
0.50.0-
?...\
pH
B
3.0'
2.5, ~
--x-- oxalicacid
I ~
acid
succhlc acid
- - l - - glutaricacid
----0--9d~ acU
-~
~zo-
" ~
1.5
--o-- p~lc =u
a
I
1.o
0.5. _
~s
"
;.o
0.0"
zo
;5
3'.o
"
~5
"
5'.o
pH
the retention volume obtained for a neutral compound like methanol. This behavior may be explained partially by hydrophobic interaction between
the phenyl groups of the spacers and the analytes.
However, also adsorption phenomena between the
silica gel and the solutes as reported by Ohta et al.
[14] should be considered.
j,,,,._
4
27
4. Conclusion
2
28
A
1
3
4
mlautm
Acknowledgments
References