Shimadzu HPLC Columns

C190-E043C
Shimadzu
HPLC Columns
Shimadzu
High Per for mance Liquid Chromatograph
CONTENTS
1 Classification of Shimadzu HPLC Columns
Classification by Support Material 4
Classification by Elution Mode 5
Classification by Column Dimensions 6
Classification by ODS Columns 7
2 Guide for Selection of Operational Conditions

Amino Acids 8
Peptides and Proteins 9
Nucleic Acids 10
Sugars 11
Vitamins 12
Carboxylic acids 13
Ion 14
3 Shimadzu HPLC Columns

Shim-pack
Shim-pack VP-ODS for GLP/GMP compliance 15
Shim-pack HT-ODS 19
Shim-pack FC-ODS 20
Shim-pack HRC, MRC, PRC 21
Shim-pack CLC 24
Shim-pack PREP 28
Shim-pack FLC, SBC, MBC 30
Shim-pack Ion Exchange Columns 33
Shim-pack GPC 38
Shim-pack Columns for Gel Filtration/Ion Exclusion
Chromatography 41
Shim-pack IC 45
Shim-pack HPC 47
Shim-pack SPC 49
Shim-pack GRD, Pre-column 50
Dedicated Shim-pack Columns 51
Shim-pack Preparative Columns 53
Shim-pack BIO (T) 57
Asahipak
Asahipak ODS 59
Asahipak GS 62
Asahipak ES 64
Asahipak NH2P 65
CAPCELL PAK 66
69
Zorbax
STR
STR ODS Series 70
4 Application Data 73
5 Parts for Flow line
Plumbing parts (Connecting Parts) 83
PEEK plumbing parts 85
Shimadzu Amino Acid Analysis kit for amino acid
analytical system 87
Disposable Filter 88
6 Shim-pack Columns 90
7 Sample and data 98
3
1 Classification of Shimadzu HPLC Columns

Classification by Support Material
The solid supports of packing materials for HPLC are classified into the following two groups with the following features.
Silica Gel Type
High mechanical strength, hence easily pulverized into very fine particles.
High efficiency
Packing Materials
for HPLC
Polymer Type
High stability in wide pH range
The Shimadzu HPLC columns can be classified into two groups as follows:
Shim-pack Series (ODS, C8, TMS, CN, Phenyl, NH2, SIL)
CAPCELL PAK Series (C18, C8)
Silica Gel Type
Zorbax Series(ODS, C8, TMS, NH2, CN, SIL, etc.)

STR Series(ODS)
Shimadzu HPLC
Columns
Polymer Type
Polystyrene
Shim-pack Series (Amino, ISC, ISA, SCR, IC, ION, GPC)
Polyacrylate
Shim-pack Series(PA, IC)
Polyvinyl alcohol
Asahipak Series(GS, ES, ODP)
Classification by Elution Mode

The
two groups are futher classified as follows:

SIL(Shim-pack, Zorbax)Phospholipids
Normal phase elution
NH2(Shim-pack, Zorbax) Saccharides, tocopherols

CN(Shim-pack, Zorbax) Steroid hormones
ODS(C18)(Shim-pack, Zorbax, CAPCELL PAK, STR)

C8(Shim-pack, Zorbax, CAPCELL PAK)
Silica Gal Type
Reversed phase elution
CN(Shim-pack, Zorbax)
Low molecular-weight compounds
TMS(Shim-pack, Zorbax)
Phenyl(Shim-pack)
Shim-pack WAX(Weakly basic anion exchange) Nucleotides,proteins

Shim-pack WCX(Weakly acidic cation exchange) Proteins
Ion exchange
Zorbax SAX(Weakly basic anion exchange)Nucleotides
Zorbax SCX(Weakly acidic cation exchange)Catecholamines
Gel filtration
Shim-pack DiolProteins
Hydrophobic Interaction
Chromatography
Shim-pack HPCProteins, nucleic acids
Affinity Chromatography
Shim-pack AFCProteins, nucleic acids
Reversed phase elution
Asahipak ODP-50(Polyvinyl alcohol)Peptides, proteins, low-molecular-weight compounds
Shim-pack Amino, ISC(Polystyrene)Amino acids, guanidines

Shim-pack ISA(Polystyrene)Saccharides
Polymer Type
Ion exchange
Shim-pack IC(Polyacrylate, Polystyrene)Inorganic ions

Shim-pack PA(Polyacrylate)Proteins, nucleic acids
Asahipak ES(Polyvinyl alcohol)Proteins
Ligand exchange
Shim-pack SCR(Sulfonated polystyrene)Saccharides
Ion exclusion
Shim-pack SCR-101H, 102H(Sulfonated polystyrene)Organic acids
Gel Filtration
Asahipak GS(Polyvinyl alcohol)Water-soluble high polymers
GPC
Shim-pack GPC(Polystyrene)Synthetic high polymers, rubbers
Classification by Column Dimensions

HPLC
columns are classified as analytical columns and preparative columns according to the dimensions.
Analytical columns are further classified into several groups.
(Column size)
(Representative Shim-pack series)
20mm i.d.25cm
PREP series
30mm i.d.25cm
PREP(K) series
50mm i.d.25cm
PREP(L) series
4.0mm i.d.15cm or 25cm
ISC series etc.
CLC(M) series
6.0mm i.d.15cm
HRC,CLC series
8.0mm i.d.30cm
GPC series
Fast-LC
4.6mm i.d.15cm
FLC series
Small bore
2.5mm i.d.15cm
SBC series
Micro bore
MBC series
Semi-preparative
Preparative
column
Large scale preparative
HPLC
columns
Standard
Analytical
column
Fast analysis
Moble phase consumption
is less than 1/3
Moble phase consumption
is less than 1/20
Compared with column 4.6mm i.d.
You
can choose 20mm i.d., 30mm i.d., 50mm i.d. preparative columns in accordance with the scale of preparative.
Each is 25cm long.
As standard size analytical columns, 48mm i.d. and 1530cm long columns are most widely used and are available in a
wide variety.
As other special sizes, analytical columns such as Fast-LC columns, Micro bore columns and small bore columns are
available. (Refer to P.26)
Micro bore columns

Shimadzu
MBC series micro bore columns are only 1.0mm in inner diameter.
mobile phase consumption is only about 1/20 that required in a 4.6mm i.d. column, because the same linear velocity
can be obtained with about 1/20 flow rate.
The sample injector, the detector flow-thru cell, and connecting pipes of a small capacity must be used.
The
Small bore columns

Shimadzu
SBC series small bore columns are 2.5mm in inner diameter.

mobile phase consumption is about 1/3 compared with a 4.6mm i.d. column.
Though this series columns are compatible with an ordinary HPLC, it is better to use small capacity units.
The
Fast-LC columns
Shimadzu
FLC series fast LC columns are packed with 3m dia.

Column packing is only 5cm long.
They are compatible with an ordinary HPLC and permit very fast analysis.
Classification by ODS Columns

ODS columns
ODS columns are packed with packing material having octadecyl group chemically-bonded on the surface and are most
popularly used in HPLC.
ODS columns can have different separation properties, according to the state of end-capping and the characteristics of the
silica gel used as the solid support. They can also be different with the brand.
The delicate differences in separation properties can be utilized to achieve some difficult analysis.
Shimadzu provides various types of ODS columns to ensure a wide field of application.
Silica Gel Type
Shimadzu HPLC
ODS Columns
Polymer Type
Shim-pack HRC-ODS
Analysis for general use
Shim-pack CLC-ODS
Shim-pack FLC-ODS
Fast-LC
Shim-pack MBC-ODS
Micro bore LC
Shim-pack SBC-ODS
Small bore LC
Shim-pack PREP-ODS,
-ODS(H), -ODS(L), -ODS(K)
Preparative
STR ODS
CAPCELL PAK C18
Polymer-coated
and end-capped
Zorbax ODS
Non end-capped
Asahipak ODS
Polyvinyl alcohol gel

t o i nt roduc e C18
End-capped
Method to Introduce C18 Groups
Selection of ODS Columns

As standard analytical columns, we provide Shim-pack HRC-ODS, CLC-ODS, STR ODS, Zorbax ODS, CAPCELL PAC C18,
Asahipak ODP.
The following table is a rough guide for selecting ODS columns.
Column name
Features
Mobile phase pH range
Shim-pack HRC-ODS
Shim-pack CLC-ODS
STR ODS
End-capped to minimize the influence of residual silanol

groups and eliminate tailing of basic compounds.
27.5
Zorbax ODS
Utilizes the influence of residual silanol compounds.
27.5
CAPCELL PAK C18
Silica gel solid support is coated with silicone polymer to

improve stability in basic mobile phase.
210
Asahipak ODP-50
Applicable to mobile phase up to pH13.

The selectivity is different from that of silica gel types.
213
2 Guide for Selection of Operational Conditions

Amino Acids
Amino acids are amphoteric compounds having carboxyl groups and amino groups.
Amino acids are classified as acidic, neutral, and basic according to the number of
these groups. Also they are classified as aliphatic type and aromatic type, depending
on the group at the position of R.
Separation mode
The
It
cation exchange chromatography is most popular.

is classified into two methods, Li type and Na type.
Li type
Biological amino acids
Na type
Protein-hydrolyzed amino acids
Cation exchange
The
Li type is recommended for analysis of biological amino acids. It is also used for separating glutamine and glutamic acid.
phase elution mode can separate particular type of amino acids but cannot separate all free amino acids.
It is quite effective for separating amino acids derivatized by precolumn derivatization.
Reversed
Detection Method
The
UV absorption method which utilizes the absorption of carboxyl groups at 200210nm is not widely used though it is
useful for detecting some type of amino acids.
Pre-column derivatization and post-column derivatization methods are often used for detecting amino acids.
Pre-column method
React before injects in columns. Special accessories are not needed.
Post-column method
Continuously add reaction solution to column elution.

Ease of automation and suited for routine analysis.
Derivatization
method
Pre-column
derivatization method:
The sample solution is derivatized before entering the analytical column. Combination of the pre-column derivatization
method and the reversed phase elution mode provides rapid analysis of amino acids produced in hydrolysis of proteins.
The reagents for pre-column derivatization such as OPA(ortho-phthalaldehyde) are commercially available.
Post-column deribatization method:
The derivatizing reagent is continuously added to the column effluent. This method has the noteworthy advantage that the
entire operation can be easily automatized. Combination of the post-column derivatization method and the cation exchange
method permits analysis of wide variety of amino acids ranging from free amino acids to amino acids produced in hydrolysis
of proteins. The representative reagents used in the post-column derivation method are OPA (for fluorescing) and ninhydrin.
Column
(Sample)
(Separation mode)
(Typical column)
(Detection)
Shim-pack Amino-Li
Free amino acids
Amino Acids
Cation exchange
Cation exchange
Post-column derivation
and fluorescing with OPA
Shim-pack Amino-Na
Protein-hydrolyzed
amino acids
Reversed-phase
Shim-pack HRC-ODS
CAPCELL PAK C18,
etc.
Pre-column derivatization
and fluorescing with OPA
Shimadzu HPLC Amino Acid Analysis System Application Data Book (C190-E004)
Peptides and Proteins

Peptides and proteins consist of amino acids which are combined by acid-amide bonding. Their ionic and hydrophobic
properties are different depending on the type and the number of constituting amino acids.
Proteins have high-degree structures with various combinations.
Separation mode
Peptides
and proteins are generally analyzed by one of the following methods.
Gel filtration
Separation by molecular sizes
Ion exchange
Separation by molecular electric charge
Reversed phase
Separation by molecular hydrophobic properties
Hydrophobic
Detection Method
The
UV spectrophotometric method is generally used. Detection at 280nm is effective in most cases.

at about 210nm is used for detection of peptides consisting of aliphatic amino acids and detection of lowconcentration peptides and proteins.
Detection
Column
(Separation mode)
Gel filtration
(Pepresentative column)
Shim-pack Diol
(Refer to P.41)
Asahipak GS
(Refer to P.62)
Shim-pack WCX-1
Shim-pack PA-CM
Cation exchange
(Refer to P.33)
Shim-pack PA-SP
Asahipack ES-502C
(Refer to P.64)
Shim-pack WCX-2
Peptides
Proteins
Shim-pack PA-DEAE
Anion exchange
Reversed phase
Hydrophobic
(Refer to P.33)
Shim-pack PA-QA
Asahipack ES-502N
(Refer to P.64)
Shim-pack HRC-ODS
(Refer to P.21)
Shim-pack CLC-ODS
(Refer to P.23)
Asahipack ODP-50
(Refer to P.59)
CAPCELL PAK C18
(Refer to P.66)
STR ODS
(Refer to P.70)
Shim-pack HPC
(Refer to P.47)
Nucleic Acids
Nucleic acids play important roles in organism. They are classified into the following three groups:
Nucleic acid base
Nucleoside
(Nucleic acid base sugar)
Nucleotide
(Nucleoside phosphorus)
Separation mode
Nucleic
acid bases and nucleosides are analyzed in the cation exchange mode or the reversed phase elution mode.
are analyzed in the anion exchange mode in most cases. The reversed phase elution is also useful with
suitable selection of mobile phases.
In analysis of high molecular weight samples such as oligonucleotides, DNA, and RNA, gel filtration mode, hydrophobic
mode, and ion exchange mode are selectively used.
Nucleotides
Detection Method
Nucleic
acids and related compounds generally have strong ultraviolet absorption, therefore UV spectrophotometric
detection (250260nm) is used in most cases.
Column
(Separation mode)
(Column)
Cation exchange
Shim-pack WCX-1
(Refer to P.24)
Reversed phase
Shim-pack HRC-ODS
Shim-pack CLC-ODS
Asahipak ODP-50, GS
(Refer to P.14)
(Refer to P.16)
(Refer to P.59)
Anion exchange
Shim-pack WAX-1
(Refer to P.33)
Nucleic acid bases

and nucleosides
Nucleic Acids
Nucleotides
Reversed phase
Gel filtration
Anion exchange
DNA and RNA
Hydrophobic
10
Shim-pack HRC-ODS
Shim-pack CLC-ODS
Asahipak GS
Shim-pack Diol
Asahipak GS
Shim-pack WAX-2
Shim-pack PA-DEAE
Shim-pack PA-QA
Asahipak ES-502N
Shim-pack HPC
(Refer to P.14)
(Refer to P.30)
(Refer to P.62)
(Refer to P.41)
(Refer to P.62)
(Refer to P.33)
(Refer to P.64)
(Refer to P.47)
Sugars
Sugars such as glucose and sucrose are very common and are available in a
great variety.
Pentose
(five carbon atoms per molecule)
Xylose, arabinose, etc.
Hexose
(six carbon atoms per molecule)
Glucose, fructose, galactose, etc.
Disaccharides
combination of two monosaccharides
Sucrose, maltose, etc.
Oligosaccharides
combination of 2~10 monosaccharides
Dextrin, etc.
Polysaccharides
Polymers consisting of
many monosaccharides
Starch, cellulose, etc.
Monosaccharides
Sugars
Separation mode
Sugars
are generally analyzed by one of the following methods.
Partition
Separation by the interaction based on partition
Separation of mono- ~15-saccharide
Poor separation
of epimers
Ligand exchange
Separation by the interaction between and

counter-ion on sulfonic acids
Separation of monosaccharides and

separation of sugars from alcohols
Poor separation of diand higher saccharides
Anion exchange
Separation by the interaction based on ion

exchange of sugar-borate complex
Separation of mono-~trisaccharides
Time consuming
Separation by the size of the molecular
Measurement of molecular
weight distribution
Components of the
same molecula weight
cannot be separated.
Gel filtration
Detection Method
A
UV spectrophotometric detector is not used because sugars absorb radiation only around 190nm.
refractive index detector is generally used in the analysis of sugars.
When sugar content is low and impurity content is high, as in the case of brewage products and natural materials, the postcolumn derivatization method is used.
A
Column
Monosaccharides
(Separation mode)
Ligand exchange
Anion exchange
Sugars
(Column)
Shim-pack SCR-101N
Shim-pack SCR-101C
Shim-pack SCR-101P
(Refer to P.41)
Shim-pack ISA-07
(Refer to P.33)
Shim-pack HRC-NH2
Shim-pack CLC-NH2
Asahipak NH2P
(Refer to P.21)
Asahipak GS
(Refer to P.62)
Mono~ trisaccharides
Partition
(Refer to P.24)
(Refer to P.65)
Oligosaccharides
Polysaccharides
Gel filtration
11
Vitamins
Vitamins are classified as water-soluble type and oil-soluble type.
B1 (thiamine), B2 (riboflavin), B6 (pyridoxine),
B12 (cyanocobalamine), BT (carnitine), nicotinic acid,
nicotinamide
Vitamin B
Water-soluble
vitamins
Vitamin C
Ascorbic acid
Vitamin H
Biotin acid
Vitamin M
Folic acid
Vitamin P
Hesperidin
Pantothenic acid
Vitamin A
Retinol
Vitamin D
Oil-soluble
vitamins
D2 (ergocalciferol), D3 (cholecalciferol)
Vitamin E
Tocopherol
Vitamin K
Separation mode
Both
water-soluble and oil-soluble vitamins are analyzed in the reversed phase elution mode.
oil-soluble vitamins are analyzed in the partition mode.
Some
Detection Method
The
UV spectrophotometric method is generally used: the spectrofluorometric method is used for some vitamins.
UV (325nm)
Vitamin A (retinol)
Fluo (Ex. 340nm, Em. 460nm)
Vitamin B1 (thiamin)
UV (270nm)
Vitamin B2 (riboflavin)
UV (270nm)
Vitamin B6 (pyridoxine)
UV (290nm)
Vitamin B12 (cyanocobalamine)
UV (280nm)
UV (210nm)
Vitamin BT (carnitine)
RI
Vitamin C (ascorbic acid)
UV (245nm)
Vitamin D
UV (265nm)
UV (295nm)
Vitamin E (tocopherol)
Fluo (Ex. 295nm, Em. 325nm)
Vitamin H (biotin)
UV (210nm)
Vitamin K
UV (250nm)
Vitamin M (folic acid)
UV (280nm)
Vitamin P (hesperidin)
UV (265nm)
Note: The above wavelength values are approximate; they can be influenced by the state of mobile phase.
Column
(Separation Mode)
Water-soluble vitamins
Reversed phase
(Ion-pair)
Partition
(Vitamin C)
Vitamins
Reversed phase
Oil-soluble vitamins
Partition
(Vitamin E isomers)
12
Shim-pack HRC-ODS
Shim-pack CLC-ODS
STR-ODSH
(Refer to P.21)
(Refer to P.24)
(Refer to P.70)
Shim-pack HRC-NH2
Shim-pack CLC-NH2
(Refer to P.21)
(Refer to P.24)
Shim-pack HRC-ODS
Shim-pack CLC-ODS
STR-ODSH
Shim-pack HRC-NH2
Shim-pack CLC-NH2
(Refer to P.21)
(Refer to P.24)
(Refer to P.70)
(Refer to P.21)
(Refer to P.24)
Carboxylic acids
Carboxylic acids are organic compounds, including carboxyl group and stand for acids
properties. These are classified as follows.
Saturation aliphatic carboxylic acids

Non saturation aliphatic carboxylic acids
Acetate, stearic acid,

succinic acid, etc.
Acrylic acid, linoleic acid,
fumaric acid, etc.
Aliphatic carboxylic acids

Hydroxy carboxylic acids
Lactic acid, malic acid,

citric acid, etc.
Keto carboxylic acids
Pyruvic acid, oxalacetic

acid, etc.
Carboxylic acids
Benzuic acid, mandelic

acid, etc.
Aromatic carboxylic acids
Carboxylic acids are also classified into mono, di, poly (more than 3) carboxylic acids in accordance with the number of
carboxyl group.
Separation mode
Ion exclusion chromatography, anion ion exchange chromatography and reversed phase chromatography can be used in
accordance with the dissociation and the side chain of carboxylic acid.
Ion exclusion chromatography is suitable for the analysis of high aqueous carboxylic acids and used widely to analyze food
and cultivation solutions.
Anion exchange chromatography is a basic mode of carboxylic acids having the properties of acids and has an advantage
of separating from neutral components.
Reversed phase chromatography is suitable for the separation of fatty acid and aromatic carboxylic acids.
Detection Method
Carboxylic
acids have slight absorbency at around 205nm, therefore UV spectrophotometoric detector can be used. As far
as aromatic carboxylic acids and aliphatic carboxylic acids are concerned, these absorbencies are relatively strong so that
can be detected with a high sensitivity.
However, the absorbency coefficients of carboxylic acids are generally small and the selectivity of the detection are poor.
As a result, there are cases where it is difficult to analyze some samples. Therefore, pre-column derivatization using label
to carboxyl group might be used.
Post-column conductivity detector making use of separating carboxul group makeks it possible to improve selectivity.
Column
Saturation aliphatic
carboxylic acids
Reversed phase
Shim-pack HRC-ODS
Shim-pack CLC-ODS
STR ODS etc.
Ion exclusion
Shim-pack SCR-102H
Shim-pack SCR-101H
Non saturation aliphatic

carboxylic acids
Aliphatic carboxylic acids

Hydroxy carboxylic acids
Carboxylic acids
Keto carboxylic acids
Aromatic carboxylic acids
Anion exchange
Shim-pack WAX-1
Shim-pack IC-A1
Reversed phase
Shim-pack HRC-ODS
Shim-pack CLC-ODS
STR ODS
13
Ion
Ions are classified as follows. Mainly inorgainic ion and low molecale organic ion are the object of ion chromatography
analysis.
Organic ion
Ion
Anion ion
Carboxylic acids
Sulfonic acids
Cation ion
Amine
Amphoteric ion
Amino Acide
Anion ion
Inorganic anion ion
Cation ion
Alkaline metals
Alkaline-earth metals
Transition metals
Rare-earth metals
Inorganic ion
Separation mode
Ion exchange is a main method, but sometimes ion pair and reversed phase method are used.
Detection Method
In
In
IC (Ion chromatography) analysis, generally conductivity detector can be used with a high sensitive.
special ions, many selectible detector can be used to make use of each chemical property.
(Detection Method)
(Analytical objection)
Inorganic ions
CDD
Post-column pH
UV
VIS
Carboxylic acids
Indirect absorbance method
Inorganic ions
Direct Absorbance method
UV absorbance ion

NO 2 , NO 3 Br , I
Carboxylic acid
Post-column method
Transition metals, Rare-earth metals
RI
Boricacid, Silicate
L-ECD
2
NO
4 , SO 4 etc.
RF
Post-column method
NH
4
Column
(Separation mode)
Ion exchange
(Representative column)
Shim-pack IC
(Refer to P.45)
Ion pair
Shim-pack HRC-ODS
Shim-pack CLC-ODS
(Refer to P.21)
(Refer to P.24)
Ion exclusion
Shim-pack SCR-101H
Shim-pack SCR-102H
(Refer to P.41)
Shim-pack HRC-ODS
Shim-pack CLC-ODS
(Refer to P.21)
(Refer to P.24)
Ion
Reversed phase
Shimadzu Ion Chromatography System Application DataBook (C190-E002)
14
Shim-pack

Shim-pack VP-ODS for GLP/GMP compliance
For development or validation of analytical method, manufacturing uniformity of columns is increasing.
Shim-pack VP-ODS has been developed to meet such expectations.
VP-ODS Series
Excellent manufacturing uniformity
Analytical Column Shim-pack VP-ODS

Cat. No.
I.D.length (mm)
To minimize column-to-column performance deviation of

ODS columns, silica-bases, surface treatment and packing
228-34937-91
228-34937-92
4.6150
4.6250
procedures are strictly controlled respectively and only the
228-34937-93
228-34937-94
6.0150
2.0150
customers. For development or validation of method, it would
228-34937-95
228-34937-96
228-34937-97
2.0250
4.6150, a set of 3pcs.
products that passed the quality criteria are delivered to

be efficient to run the test with a set of three columns with
packings of different batches.
An analytical column set consists of three columns whose packings

(Silica-based) are from different production batches.
Shim-pack VP-ODS
Guard Column Shim-pack VP-ODS

Cat. No.
I.D.length (mm)
228-34938-91
4.610
Incl. exchangeable cartridge 2pcs.
228-34938-92
A holder for I.D. 4.6mm
228-34938-93
2.05
228-34938-94
Batch C
A company's column
Batch B
Batch A
Guard column consists of a cartridge and holder. Holders can be used

repeatedly.
Short Columns for Analytical/Preparative

Cat. No.
228-36849-91
228-36849-92
228-36849-93
I.D.length (mm)
Remarks
4.650
5m diameter,
2050
12nm (120) pore
20100
porous silica ODS
Batch C
Peaks
. N-acetylprocainamide
. Phenol
Batch B
Batch A
Fig. 1
Comparison of lot-to-lot repeatability (Silica-based material)
15
Shim-pack

VP-ODS Series

Cat. No.
I.D.length (mm)

228-34937-91
228-34937-92
4.6150
4.6250
228-34937-93
228-34937-94
6.0150
2.0150
228-34937-95
228-34937-96
228-34937-97
2.0250


Shim-pack VP-ODS

Cat. No.
I.D.length (mm)
228-34938-91
4.610
228-34938-92
228-34938-93
2.05
228-34938-94
Batch C
A company's column
Batch B
Batch A

repeatedly.

Cat. No.
228-36849-91
228-36849-92
228-36849-93
I.D.length (mm)
Remarks
4.650
5m diameter,
2050
12nm (120) pore
20100
porous silica ODS
Batch C
Peaks
. Phenol
Batch B
Batch A
Fig. 1
15
Shim-pack

VP-ODS Series

Cat. No.
I.D.length (mm)

228-34937-91
228-34937-92
4.6150
4.6250
228-34937-93
228-34937-94
6.0150
2.0150
228-34937-95
228-34937-96
228-34937-97
2.0250


Shim-pack VP-ODS

Cat. No.
I.D.length (mm)
228-34938-91
4.610
228-34938-92
228-34938-93
2.05
228-34938-94
Batch C
A company's column
Batch B
Batch A

repeatedly.

Cat. No.
228-36849-91
228-36849-92
228-36849-93
I.D.length (mm)
Remarks
4.650
5m diameter,
2050
12nm (120) pore
20100
porous silica ODS
Batch C
Peaks
. Phenol
Batch B
Batch A
Fig. 1
15
Packing characteristics of Shim-pack VP-ODS

Silica particles
Entirely porous, spherical silica particles with a high purity
Particle size
5m
Pore size
12nm
Pore volume
1.25mL/g
Specific surface area
410m2/g
Trace metal content
30ppm max
Percentage of ODS
20%C
Carbon content
ODS functional group
Mono-functional
End-capping
Used
Number of theoretical plates and column pressure

The higher NTP is and the lower the
pressure is, the easier to handle the
Column C
column is. Shim-pack VP-ODS

shows superior performance shown
Column B
by NTP per 1MPa.
Column A
1920
1060
1590
2560
Shim-pack VP-ODS
0
500
1000
1500
2000
2500
3000
NTP per 1MPa

Column size: 4.6150mm, Mobile phase: methanol/water70:30,
Flow rate: 1.0mL/min. Sample: naphthalene
Excellent peak shapes

Peak
. oxine
Shim-pack VP-ODS
16
Other column
Peaks
. propranolol
. acenaphthene
. amitriptyline
Other column
Shim-pack VP-ODS
Fig. 2
Fig. 3
Excellent peak shape of coordination compounds is achieved by the

packing base material with less metal impurities.
Excellent peak shapes of basic compounds are achieved by thorough

effective end-capping of the packings.
Shim-pack
Certificate of Analysis (Quality certificate)

Shim-pack VP-ODS is shipped with three types of certificate of Analysis to support method validation for HPLC analysis.
1. Certificate of Analysis for Packing Material (Physical Properties)
2. Certificate of Analysis for Packing Material (Chromatographic Performance)
3. Shim-pack Column Performance Report
Phyical Properties of Shim-pack VP-ODS
Chromatographic Performance
of Shim-pack VP-ODS
Shim-pack Column Performance

Report
Application Data
Peaks
. Caffeine
. Aspartame
. Benzoic acid
Peak
. Hesperidin
Fig. 4 Analysis of Aspartame in Soft Drink
Fig. 5
Analysis of Hesperidine in Orange Juice
17
Peaks
. Hypoxanthine
. IMP
. Inosine
. AMP
. ADP
. ATP
Peaks
. Nicotinamide
. Riboflavin
phosphate
. Phridoxine
. Caffeine
. Thiamine
2
1
4
Fig. 6
Analysis of Nucleic Acid in Tuna Meet
Fig. 7
Analysis of Vitamin B Group in Soft Drink
Peak
. Chlorhexidine
Peak
. Berberine
Fig. 8
Analysis of Chlorexidine in Ointment
Fig. 9
Peak
. Oxine
Analysis of Berberine
Peaks
. Formaldehyde
. Acetaldehyde
. Acetone
. Acrolein
. Propionaldehyde
. Crotonaldehyde
. 2-butanone
11
8 9
10
12
.
.
10.
11.
12.
13.
Methacrolein
Butyraldehyde
Benzaldehyde
Veleraldehyde
m-tolualdehyde
Hexanaldehyde
13
Fig. 10
18
Analysis of Oxine Standard
Fig. 11
Analysis of DHPH-Aldehydes, Ketones group Standard
Shim-pack
Shim-pack HT-ODS High-speed HPLC columns
Best Partner For High Throughput Analysis
Recently, the demand for high throughput analysis has
increased. For HPLC methods, it is essential to save analysis
time and to obtain reliable results. To meet such requirements,
Shimadzu has developed the High Throughput (HT) column
packed with very fine non-porous, high purity silica particles.
With this column, it is possible to realize ultra high speed
analyses (Analysis times within 1 minute.) Moreover, quality
certificates for the uniformity of the packing material are
provided for each column so it's easy to comply with
validation requirements.
We are sure this column makes your analyses faster and more
reliable.
High speed analysis
0.150
Flow rate: 1.5mL/min
With 2m and non-porous packing material, it is possible to

drastically reduce the gradient analysis run times while
obtaining the same peak resolution. It is possible to finish an
analysis within 1 minute. Shim-pack HT-ODS provides the
capability of ultra high speed and high resolution analysis.
Gradient analysis cycle: 3.5min
Peaks :
0.100
1: methyl paraben
2: ethyl paraben
3: n-poropyl parben
4: n-butyl paraben
0.050
5: biphenyl
6: chrysene
0.000
0.0
0.4
0.8
1.2
min
Validation compliance
We certify the reliability of the analysis data with a 3 column
set made from different lots and attach quality certificates for
the uniformity of the packing material.
The highest performance with Shimadzu LC-2010,

High-throughput HPLC
The combination of the Shim-pack HT-ODS and the
Shimadzu LC-2010 is sure to help you to achieve additional
high throughput in your research and analyses.
19
High resolution by Non-porous silica

Average resolution
The right chart shows the time and the resolution in gradient
analyses by a column packed with porous silica and one
packed with non-porous silica. Non-porous silica shows
higher resolution.
The time to obtain the same resolution with a non-porous
column is less than the time required when using a porous
silica column.
Porous
15
Non-Porous
10
0
0
10
15
20
Time of gradient (min)

Time of gradient and resolution
Benefit of non-porous silica column - High

speed and reliable Analysis
Porous silica
7.5 min gradient
The right chromatograms show gradient analyses with the

same resolution by non-porous packed column and porous
packed column. Non-porous column realizes high speed and
reliable analysis.
Non-porous silica column can save your precious time and
provide you more satisfactory performance.
Non-porous
3 min gradient
min
Comparison of retention time between non-porous

and porous columns.
Shim-pack HT-ODS
Cat. No.
Description
I.D.length (mm)
228-37681-91
Shim-pack HT-ODS
4.630
2m non-porous silica ODS
228-37681-92
Shim-pack HT-ODS
(3 column kit)
4.630
2m non-porous silica ODS

A kit of columns with packings
from three different production lots
Remarks
Shim-pack FC-ODS
High throughput and high resolution column packed with 3m porous high purity silica.
Applicable to wide application range from high thoughput precisely separation analysis of complex matrix samples.
Shim-pack FC-ODS
20
Cat. No.
Description
I.D.length (mm)
221-40511-91
Shim-pack FC-ODS
4.630
Remarks
221-40511-92
Shim-pack FC-ODS
4.675
For shorter analysis time
221-40511-93
Shim-pack FC-ODS
4.6150
For high separation
For fast analysis
Shim-pack
Shim-pack HRC, MRC, PRC
High
quality and outstanding performance ensured by stringent quality control.

The same silica gel solid support is used in all the columns of this series.
Adsorption even of basic compounds is completely eliminated by the Shimadzu's original secondary silylation method
(except the -SIL series which is packed with silica particles without any surface treatment.
The same packing material is used in the analytical and preparative columns, so that the operational parameters for
analytical runs may be easily copied for preparative work.
Analysis (5m)
HRC Series
4.6mm i.d.15cm
4.6mm i.d.25cm
6.0mm i.d.15cm
Scale-up
PRC(H) Series
High separation preparation (5m)
20mm i.d.25cm
PRC Series
Preparation (15m)
20mm i.d.25cm
30mm i.d.25cm
50mm i.d.25cm
Scale-up
MRC Series
Operational conditions for preparation (15m)
6.0mm i.d.25cm
21
Shim-pack HRC
Column name
Shim-pack HRC-SIL
Shim-pack HRC-ODS
Shim-pack HRC-C8
Shim-pack HRC-TMS
Shim-pack HRC-NH2
Shim-pack HRC-CN
Stationary phase
Particle dia. (m)
Silica
Octadecyl group
Octyl group
Trimethyl group
Aminopropyl group
Cyanopropyl group
Separation mode
Absorption
Reversed phase
Reversed phase
Reversed phase
Dimensions
Cat. No.
4.6mm i.d.15cm
228-23460-91
4.6mm i.d.25cm
228-23460-92
6.0mm i.d.15cm
228-23460-93
4.6mm i.d.15cm
228-23463-91
4.6mm i.d.25cm
228-23463-92
6.0mm i.d.15cm
228-23463-93
4.6mm i.d.15cm
228-24376-91
4.6mm i.d.25cm
228-24376-92
6.0mm i.d.15cm
228-24376-93
4.6mm i.d.15cm
228-24377-91
4.6mm i.d.25cm
228-24377-92
6.0mm i.d.15cm
228-24377-93
4.6mm i.d.15cm
228-24378-91
Reversed, normal, ion exchange 4.6mm i.d.25cm
228-24378-92
6.0mm i.d.15cm
228-24378-93
4.6mm i.d.15cm
228-24379-91
Reversed phase, Normal phase 4.6mm i.d.25cm
228-24379-92
6.0mm i.d.15cm
228-24379-93
Guard Column for HRC

Column name
Shim-pack GHRC-SIL
Shim-pack GHRC-ODS
Particle dia. (m)
Dimensions
Cat. No.
Silica
4.0mm i.d.1cm
228-23462-91
Octadecyl group
4.0mm i.d.1cm
228-23465-91
Octyl group
4.0mm i.d.1cm
228-24386-91
Shim-pack GHRC-TMS
Trimethyl group
4.0mm i.d.1cm
228-24387-91
Shim-pack GHRC-NH2
Aminopropyl group
4.0mm i.d.1cm
228-24388-91
Shim-pack GHRC-CN
Cyanopropyl group
4.0mm i.d.1cm
228-24389-91
Shim-pack GHRC-C8
22
Stationary phase
Shim-pack
Shim-pack PRC
Column name
Stationary phase
Particle dia. (m)
Separation mode
Shim-pack PRC-SIL
Shim-pack PRC-SIL(K)
Shim-pack PRC-SIL(L)
15
Silica
Shim-pack PRC-SIL(H)
Shim-pack PRC-ODS(L)
Octadecyl group
Cat. No.
228-23461-93
30mm i.d.25cm
228-23461-94
50mm i.d.25cm
228-23461-95
20mm i.d.25cm
228-23461-91
20mm i.d.25cm
228-23464-93
15
30mm i.d.25cm
228-23464-94
50mm i.d.25cm
228-23464-95
Adsorption
Shim-pack PRC-ODS
Shim-pack PRC-ODS(K)
Dimensions
20mm i.d.25cm
Reversed phase
Shim-pack PRC-ODS(H)
20mm i.d.25cm
228-23464-91
Shim-pack PRC-C8
15
20mm i.d.25cm
228-24381-93
Shim-pack PRC-C8(H)
Shim-pack PRC-TMS
Shim-pack PRC-TMS(H)
Shim-pack PRC-NH2
Shim-pack PRC-NH2(H)
Shim-pack PRC-CN
Shim-pack PRC-CN(H)
Octyl group
Reversed phase
5
15
Trimethyl group
Reversed phase
5
15
Aminopropyl group
Reversed, normal,
ion exchange
5
15
Cyanopropyl group
Reversed phase, normal phase
20mm i.d.25cm
228-24381-91
20mm i.d.25cm
228-24382-93
20mm i.d.25cm
228-24382-91
20mm i.d.25cm
228-24383-93
20mm i.d.25cm
228-24383-91
20mm i.d.25cm
228-24384-93
20mm i.d.25cm
228-24384-91
Guard Column for PRC

Column name
Shim-pack GPRC-SIL
Shim-pack GPRC-ODS
Stationary phase
Particle dia. (m)
Dimensions
Cat. No.
Silica
8mm i.d.1.5cm
228-23462-92
Octadecyl group
8mm i.d.1.5cm
228-23465-92
Octyl group
8mm i.d.1.5cm
228-24386-92
Shim-pack GPRC-TMS
Trimethyl group
8mm i.d.1.5cm
228-24387-92
Shim-pack GPRC-NH2
Aminopropyl group
8mm i.d.1.5cm
228-24388-92
Shim-pack GPRC-CN
Cyanopropyl group
8mm i.d.1.5cm
228-24389-92
Silica
15
30mm i.d.7.5cm
228-23462-93
Shim-pack GPRC-SIL(L)
Silica
15
50mm i.d.5cm
228-23462-94
Shim-pack GPRC-ODS(K)
Octadecyl group
15
30mm i.d.7.5cm
228-23465-93
Shim-pack GPRC-ODS(L)
Octadecyl group
15
50mm i.d.5cm
228-23465-94
Shim-pack GPRC-C8
Shim-pack GPRC-SIL(K)
Shim-pack MRC
Column name
Shim-pack MRC-SIL
Shim-pack MRC-ODS
Shim-pack MRC-C8
Shim-pack MRC-TMS
Stationary phase
Particle dia. (m)
Separation mode
Dimensions
Cat. No.
Silica
15
Adsorption
6.0mm i.d.25cm
228-23461-92
Octadecyl group
15
Reversed phase
6.0mm i.d.25cm
228-23464-92
Octyl group
15
Reversed phase
6.0mm i.d.25cm
228-24381-92
Trimethyl group
15
Reversed phase
6.0mm i.d.25cm
228-24382-92
Reversed, normal, ion, exchange 6.0mm i.d.25cm
228-24383-92
Shim-pack MRC-NH2
Aminopropyl group
15
Shim-pack MRC-CN
Cyanopropyl group
15
Reversed phase, normal phase
6.0mm i.d.25cm
228-24384-92
23
Shim-pack CLC
Low
cost, high-performance columns for wide field of applications.

6.0mm i.d.15cm
Shim-pack CLC
Shim-pack CLC Series
4.6mm i.d.15cm
Shim-pack CLC(M)
4.6mm i.d.25cm
Shim-pack CLC (6.0mm)

Column name
Shim-pack CLC-SIL
Shim-pack CLC-ODS
Shim-pack CLC-C8
Shim-pack CLC-TMS
Shim-pack CLC-CN
Shim-pack CLC-Phenyl
Shim-pack CLC-NH2
Stationary phase
Silica
Particle dia. (m)

5
Separation mode
Adsorption
Dimensions
6.0mm i.d.15cm
Cat. No.
228-00807-91
Octadecyl group
Octyl group
Trimethyl group
Cyanopropyl group
Phenyl group
Aminopropyl group
5
5
5
5
5
5
Reversed phase
Reversed phase
Reversed phase
Reversed-phase, normal phase
Reversed phase
6.0mm i.d.15cm
6.0mm i.d.15cm
6.0mm i.d.15cm
6.0mm i.d.15cm
6.0mm i.d.15cm
228-00808-91
228-00809-91
228-00810-91
228-00811-91
228-00812-91
Reversed, normal, ion, exchange 6.0mm i.d.15cm
228-16725-91
Also the Shim-pack VMA which is dedicated to the analysis of HVA and VMA is available.
Shim-pack CLC(M) (4.6mm)

Column name
Stationary phase
Particle dia. (m)
Separation mode
Shim-pack CLC-SIL(M)
Silica
Adsorption
Shim-pack CLC-ODS(M)
Octadecyl group
Reversed phase
Octyl group
Reversed phase
Shim-pack CLC-TMS(M)
Trimethyl group
Reversed phase
Shim-pack CLC-CN(M)
Cyanopropyl group
Phenyl group
Reversed phase
Aminopropyl group
Shim-pack CLC-C8(M)
Shim-pack CLC-Phenyl(M)
Shim-pack CLC-NH2(M)
Dimensions
4.6mm i.d.15cm
Cat. No.
228-17872-91
4.6mm i.d.25cm
4.6mm i.d.15cm
228-17872-92
228-17873-91
4.6mm i.d.25cm
4.6mm i.d.15cm
228-17873-92
228-17874-91
4.6mm i.d.25cm
4.6mm i.d.15cm
228-17874-92
228-17875-91
4.6mm i.d.25cm
4.6mm i.d.15cm
228-17875-92
228-17876-91
4.6mm i.d.25cm
4.6mm i.d.15cm
228-17876-92
228-17877-91
4.6mm i.d.25cm
4.6mm i.d.15cm
Reversed, normal, ion, exchange
4.6mm i.d.25cm
228-17877-92
228-17878-91
228-17878-92
Also the Shim-pack VMA which is dedicated to the analysis of HVA and VMA is available.
Shim-pack G(4) Series (Guard Column for CLC series)

Column name
24
Dimensions
Cat. No.
Shim-pack G-SIL(4)
4.0mm i.d.1cm
228-18270-91
Shim-pack G-ODS
4.0mm i.d.1cm
228-18246-91
Shim-pack G-C8(4)
4.0mm i.d.1cm
228-18248-91
Shim-pack G-TMS(4)
4.0mm i.d.1cm
228-18262-91
Shim-pack G-CN(4)
4.0mm i.d.1cm
228-18266-91
Shim-pack G-Phenyl(4)
4.0mm i.d.1cm
228-18264-91
Shim-pack G-NH2(4)
4.0mm i.d.1cm
228-18268-91
Shim-pack
Shim-pack HRC, CLC

Peaks
. Phosphatidylcholine (Lecithin)
. Sphingomyelin
. Lysophosphatidylcholine
(Lysolecithin)
Fig. 12
Peaks
. Nicotinic acid
. Nicotinamide
. Pantothenic acid
. Riboflavin phosphate
. Pyridoxine
. Caffeine
. Thiamine
. Riboflavin
Analysis of Phospholipids
Fig. 13 Analysis of Vitamin B
Operational Conditions
Column:
Shim-pack CLC-SIL (6.0mm i.d.15cm)
Mobile phase:
Acetonitrile/methanol/water (3/1/1)
Flow rate:
1.5mL/min.
Column temperature: 45
Detector:
UV spectrophotometric detector (205nm)
Column:
Shim-pack CLC-ODS (6.0mm i.d.15cm)
Mobile phase:
[100mM phosphate buffer solution (pH 2.1) and
1.2mM sodium octane sulfonate] /acetonitrile (9/1)
Flow rate:
1.2mL/min.
Detector:
Peaks
. Sorbic acid
. Saccharin
Fig. 14
Determination of Sorbic Acid and Saccharin
Column:
Precolumn:
Shim-pack GRD-ODS
10mM phosphoric acid buffer solution/acetonitrile (15/1)
Mobile phase:
Flow rate:
1.5mL/min.
Detector:
Peaks
. Unknown
. Cytosine
. Uracil
. Guaninc
. Adenine
. Cytidine
Fig. 16
.
.
.
10.
Peaks
. Ethyl acetate
. Ethyl propionate
. Ethyl butyrate
. Ethyl valerate
. Ethyl caproate
Fig. 15
Peaks
. Scopolamine
. Atropine
Uridine
Thymine
Adenosine
Guanosine
Analysis of Nucleic Acid Related Compounds
Column:
Shim-pack HRC-ODS (6.0mm i.d.15cm)
Mobile phase:
0.1M phosphate buffer solution (pH 2.1) and 0.2M
sodium perchlorate
Flow rate:
1.5mL/min.
Detector:
Analysis of Fatty Acid Ethyl Esters
Column:
Mobile phase:
Acetonitrile/water (1/1)
Flow rate:
1.2mL/min.
Detector:
Refractive index detector
Fig. 17
Determination of Atropine and Scopolamine
Column:
Mobile phase:
10mM phosphate buffer solution (pH 2.6)
/acetonitrile (5/1)
Flow rate:
1.5mL/min.
Detector:
25
Shim-pack HRC, CLC

Peaks
. NADH
. NAD
Peaks
. Neostigmine sulfate
. Naphazoline chloride
. Chrorpheniramine maleate
Fig. 18
Analysis of Eyewash
Fig. 19
Column:
Mobile phase:
10mM Potassium dihydrogen phosphate
/acetonitrile/phosphoric and (70/30/0.05) with 0.1%
sodium sulfonic octanate added)
Flow rate:
1.5mL/min.
Detector:
Peaks
. Vitamin A acetate
. Vitamin E
. Vitamin K 2
. Vitamin E acetate
. Vitamin A palmitate
Fig. 20
Analysis of Oil Soluble Vitamines
Column:
Mobile phase:
Methanol
Flow rate:
1.5mL/min.
Detector:
Peaks
. Aceumlnophen
. Caffeine
. Salicylamide
. Acetylsalicylic acid
. Ethenzamide
. Bromovalerylurea
Fig. 21
Fig. 22
Analysis of Phthalate Esters
26
Analysis of Analgesics/Antipyretics
Column:
Shim-pack HRC-ODS.(4.6mm i.d.15cm)
Mobile phase:
10mM Ammonium dihydrogen phosphate (pH 2.5)
/acetonitrile (4/1)
Flow rate:
1.5mL/min.
Column temperature: Ambient
Detector:
Peaks
. Vitamin D2
. Ergosterol
Peaks
. Dioctyl phthalate
. Dibutyl phthalate
. Diethyl phthalate
. Dimethyl phthalate
Column:
Shim-pack HRC-SIL (4.6mm i.d.25cm)
Mobile phase:
n-Hexane/ethanol (98/2)
Flow rate:
1.0mL/min.
Detector:
Determination of NAD and NADH
Column:
Mobile phase:
Flow rate:
1.5mL/min.
Detector:
Fig. 23
Analysis of Vitamine D2 and Ergosterol
Column:
Shim-pack HRC-SIL (4.6mm i.d.25cm)
Mobile phase:
Flow rate:
1.0mL/min.
Detector:
Shim-pack
Peaks
. -Tocopherol
. -Tocopherol
. -Tocopherol
. -Tocopherol
Peaks
. Hydrocortisone acetate
. Dexamethasone
Fig. 24
Analysis of Steroid Hormones
Fig. 25 Analysis of Tocopherols
Column:
Shim-pack CLC-CN (6.0mm i.d.15cm)
Mobile phase:
Hexane/methanol (4/1)
Flow rate:
1.5mL/min.
Detector:
Column:
Shim-pack CNC-NH2 (6.0mm i.d.15cm)
Mobile phase:
n-Hexane/isopropanol (25/1)
Flow rate:
1.5mL/min.
Detector:
Peaks
. Erythorbic acid
. Ascorbic acid
Peaks
. Glycerol
. Xylose
. Fructose
. Glucose
. Sucrose
. Maltose
. Lactose
Fig. 26
Analysis of Sugars
Column:
Shim-pack CLC-NH2 (6.0mm i.d.15cm)
Mobile phase:
Flow rate:
1.0mL/min.
Detector:
Fig. 27
Determination of Ascorbic Acid and Erythorbic Acid
Column:
Shim-pack CLC-NH2 (6.0mm i.d.15cm)
Mobile phase:
Acetonitrile/10mM phosphate buffer solution
(pH 2.6) (3/1)
Flow rate:
1.5mL/min
Detector:
27
Shim-pack PREP
Shim-pack
The
PREP Series are economical, high-performance columns for preparative LC.
columns are packed with fully porous spherical silica particles on which respective stationary phases are chemically
bonded. (Except the PREP-SIL which is packed with silica particles without any surface treatment.)
The
residual silanol groups are end-capped by the unique silylation method. (Except the PREP-SIL)
The
PREP(H) series columns are packed with 5m diameter particles (same as those used in the CLC series columns) to
permit preparative LC with high resolution.

The
PREP(H) Kit is a set of an analytical and a preparative columns which are packed with packing material (5m dia.) of
the same lot.

The
PREP, PREP(K), and PREP(L) columns are universal LC columns, having an inner diameter of 20mm, 30mm, and
50mm, respectively.
(Columns i.d.)
(Number of theoritical plates)
(Particle dia.)
5m
Shim-pack PREP(H)
More than 14,000

(More than 5,000 in NH2)
5m
Shim-pack PREP(H)Kit
More than 15,000

(Both analytical and
preparative)
15m
Shim-pack PREP
More than 5,000

(More than 4,000 in SIL
and NH2)
30mm i.d.
15m
Shim-pack PREP(K)
More than 5,000
50mm i.d.
15m
Shim-pack PREP(L)
More than 5,000
20mm i.d.
Shim-pack
PREP Series
Shim-pack PREP(H) (5m) (20mm)

Column name
Shim-pack PREP-C8(H)
Shim-pack PREP-TMS(H)
Shim-pack PREP-CN(H)
Shim-pack PREP-Phenyl(H)
Shim-pack PREP-NH2(H)
Stationary phase
Octyl group
Trimethyl group
Cyanopropyl group
Phenyl group
Aminopropyl group
Particle dia. (m)

5
5
5
5
5
Separation mode
Reversed
Reversed
Normal, reversed
Reversed
Normal, reversed, ion exchange
Dimensions
20mm i.d.25cm
20mm i.d.25cm
20mm i.d.25cm
20mm i.d.25cm
20mm i.d.25cm
Cat. No.
228-17882-91
228-17883-91
228-17884-91
228-17885-91
228-17886-91
Dimensions
4.6mm i.d.25cm
Cat. No.
Shim-pack PREP(H)Kit (5m) (20mm)

Column name
Stationary phase
Particle dia. (m)
Separation mode
Shim-pack
PREP-SIL(H)Kit
Silica
Adsorption
Octadecyl group
Reversed
Shim-pack
PREP-ODS(H)Kit
28
20mm i.d.25cm
4.6mm i.d.25cm
20mm i.d.25cm
228-17887-91
228-17888-91
Shim-pack
Shim-pack PREP (15m) (20mm)

Column name
Shim-pack PREP-SIL
Shim-pack PREP-ODS
Shim-pack PREP-C8
Shim-pack PREP-TMS
Shim-pack PREP-CN
Shim-pack PREP-Phenyl
Shim-pack PREP-NH2
Stationary phase
Silica
Octadecyl group
Octyl group
Particle dia. (m)

15
15
15
Separation mode
Adsorption
Reversed
Reversed
Dimensions
20mm i.d.25cm
20mm i.d.25cm
20mm i.d.25cm
Cat. No.
228-00814-91
228-00815-91
228-00816-91
Trimethyl group
Cyanopropyl group
Phenyl group
Aminopropyl group
15
15
15
15
Reversed
Normal, reversed
Reversed
Normal, reversed, ion, exchange
20mm i.d.25cm
20mm i.d.25cm
20mm i.d.25cm
20mm i.d.25cm
228-00817-91
228-00818-91
228-00819-91
228-17879-91
Stationary phase
Silica
Particle dia. (m)

15
Separation mode
Adsorption
Dimensions
30mm i.d.25cm
Cat. No.
228-18273-91
Octadecyl group
15
Reversed
30mm i.d.25cm
228-18319-91
Particle dia. (m)

15
15
Separation mode
Adsorption
Reversed
Dimensions
50mm i.d.25cm
50mm i.d.25cm
Cat. No.
228-18274-91
228-18320-91
Shim-pack PREP(K) (15m) (30mm)

Column name
Shim-pack PREP-SIL(K)
Shim-pack PREP-ODS(K)
Shim-pack PREP(L) (15m) (50mm)

Column name
Shim-pack PREP-SIL(L)
Shim-pack PREP-ODS(L)
Stationary phase
Silica
Octadecyl group
Shim-pack G(8), GK, and GL Guard columns

Column name
Shim-pack G-SIL(8)
Shim-pack G-ODS(8)
Shim-pack G-C8(8)
Shim-pack G-TMS(8)
Shim-pack G-CN(8)
Shim-pack G-Phenyl(8)
Shim-pack G-NH2(8)
Shim-pack GK-SIL
Shim-pack GK-ODS
Shim-pack GL-SIL
Shim-pack GL-ODS
Use
Guard column for Shim-pack PREP and PREP(H)
Guard column for Shim-pack PREP(K)

Guard column for Shim-pack PREP(L)
Dimensions
Cat. No.
8.0mm i.d.1.5cm
8.0mm i.d.1.5cm
8.0mm i.d.1.5cm
8.0mm i.d.1.5cm
8.0mm i.d.1.5cm
8.0mm i.d.1.5cm
8.0mm i.d.1.5cm
30mm i.d.7.5cm
30mm i.d.7.5cm
50mm i.d.5cm
50mm i.d.5cm
228-18270-92
228-18246-92
228-18248-92
228-18262-92
228-18266-92
228-18264-92
228-18268-92
228-18338-91
228-18321-91
228-18339-91
228-18322-91
29
Shim-pack FLC,SBC, MBC

The
Shim-pack FLC series, SBC series, and MBC series ensure higher speeds, higher sensitivity and lower mobile phase
consumption than the Shim-pack CLC series.
(Column i.d.)
(Particle dia.)
Shim-pack CLC
6mm i.d.,4.6mm i.d.
5m
Universal use
Shim-pack FLC
4.6mm i.d.
3m
Fast analysis
Shim-pack SBC
2.5mm i.d.
5m
Mobile phase consumption is less than 1/3 compared with the

Shim-pack CLC series. Applicable to ordinary HPLC systems.
Shim-pack MBC
1.0mm i.d.
10m
Mobile phase consumption is less than 1/20 compared with the

Shim-pack CLC series. Dedicated HPLC systems are required.
Comparison of Shim-pack CLC and FLC

The Shim-pack FLC series columns use 3m-diameter
packing materials; the linear velocity of mobile phase can be
increased without much lowering the column efficiency. This
feature provide very fast analyses, which are quite useful for
process control, for example.
Figure 28 shows a comparison data.
Fig. 28
Comparison Data of Shim-pack FLC and CLC
Comparison of Shim-pack CLC, SBC, and MBC

These columns have different inner diameters. A smaller
diameter column can provide a higher sensitivity, as shown in
Fig. 29.Use of a small-diameter column, however, requires use
of small-capacity sample injector and flow-thru cell; otherwise
high sensitivity will not be provided. The Shim-pack SBC
(2.5mm ida.) is applicable to ordinary HPLC systems, while
the Shim-pack MBC (1.0mm i.d.) must be used in a dedicated
HPLC system.
Fig. 29
30
Column Diameter and Peak Height
Shim-pack
Advantage of Pre-heater
In very fast LC using a Shim-pack FLC series column, a high
mobile phase flow rate can result in broadened peaks due to a
temperature gradient within the column.
A pre-heater connected between the sample injector and the
column will solve this problem as demonstrated in Fig.30.
Fig. 30
Merit of Pre-heater
Shim-pack FLC for fast LC

Column name
Shim-pack FLC-ODS
Stationary phase
Octadecyl group
Particle dia. (m)

3
Separation mode
Reversed
Dimensions
4.6mm i.d.5cm
Cat. No.
228-13375-91
Shim-pack FLC-SIL
Shim-pack FLC-CN
Shim-pack FLC-C8
Shim-pack FLC-NH2
Silica
Cyanopropyl group
Octyl group
Aminopropyl group
3
3
3
3
Adsorption
Normal, reversed
reversed
Normal, reversed, ion exchange
4.6mm i.d.5cm
4.6mm i.d.5cm
4.6mm i.d.5cm
4.6mm i.d.5cm
228-13375-92
228-13694-91
228-13695-91
228-13696-91
Particle dia. (m)

5
5
5
Separation mode
Reversed
Reversed
Adsorption
Dimensions
2.5mm i.d.15cm
2.5mm i.d.15cm
2.5mm i.d.15cm
Cat. No.
228-17268-91
228-17269-91
228-17270-91
Dimensions
1.0mm i.d.25cm
Cat. No.
228-12812-02
1.0mm i.d.50cm
1.0mm i.d.50cm
228-12812-05
228-12811-05
Shim-pack SBC small-bore columns

Column name
Shim-pack SBC-ODS
Shim-pack SBC-C8
Shim-pack SBC-SIL
Stationary phase
Octadecyl group
Octyl group
Silica
Shim-pack MBC micro-bore columns

Column name
Stationary phase
Particle dia. (m)
Separation mode
Shim-pack MBC-ODS
Octadecyl group
10
Reversed
Shim-pack MBC-SIL
Silica
10
Adsorption
Shim-pack MBC-ACN
Aminopropyl group
10
Normal, reversed
Octyl group
10
Reversed
Shim-pack MBC-C8
1.0mm i.d.100cm 228-12811-10

1.0mm i.d.25cm 228-12813-02
1.0mm i.d.50cm
1.0mm i.d.25cm
1.0mm i.d.50cm
228-12813-05
228-12814-02
228-12814-05
*Shim-pack MBC requires dedicated HPL system.
Shim-pack FLC
Peaks
Dulcoside A
. Stevioside
. Rebaudioside C
. Rebaudioside A
Peaks
. -Tocopherol
. -Tocopherol
. -Tocopherol
. -Tocopherol
Fig. 31
Analysis of Tocopherols
Column:
Shim-pack FLC-SIL (4.6mm i.d.5cm)
Mobile phase:
n-Hexane/dioxane/ethanol (98/2/0.2)
Flow rate:
2.5mL/min.
Detector:
Fig. 32
Analysis of Sweet Components in Stevia Leaf
Column:
Shim-pack FLC-NH2 (4.6mm i.d.5cm)
Mobile phase:
Flow rate:
2.0mL/min.
Detector:
31
Shim-pack SBC
Peaks
. Cytidine
. Undine
. Guanosine
. Thymidine
. Adenosine
Peaks
. Methylbenzoate
. Ethylbenzoate
. n-Propylbenzoate
. n-Butylbenzoate
Fig. 33
Analysis of Benzoic Acid Esters
Fig. 34
Column:
Shim-pack SBC-ODS (2.5mm i.d.15cm)
Mobile phase:
Methanol/water (7/3)
Flow rate:
0.4mL/min.
Detector:
Analysis of Nucleosides
Column:
Shim-pack SBC-C8 (2.5mm i.d.15cm)
50mM potassium dihydrogenphosphate methanol (93/7)
Mobile phase:
Flow rate:
0.5mL/min.
Detector:
Shim-pack SBC
Peaks
. Estrone
. Estradiol
. Estriol
Fig. 35 Analysis of Estrogens

Column:
Shim-pack SBC-SIL (2.5mm i.d.15cm)
Mobile phase:
Heptane/ethanol/methanol (90/80/2)
Flow rate:
0.6mL/min.
Detector:
Shim-pack MBC
Peaks
. Caffeine
. Bromovalerylurea
. Phenacetin
. Ethenzamide
Peaks
. -Tocopherol
. -Tocopherol
. -Tocopherol
. -Tocopherol
. Tocol
Fig. 36
Analysis of Tocopherols
Column:
Shim-pack MBC-SIL (1.0mm i.d.50cm)
Mobile phase:
Hexane/dioxane/ethanol(98/2/0.2)
Flow rate:
50L/min.
Detector:
(Micro flow-thru cell was used.)
32
Fig. 37
Analysis of Febrifuge/Analgesics
Column:
Shim-pack MBC-ODS (1.0mm i.d.25cm)
10mM phosphoric acid buffer solution/acetonitrile (3/1)
Mobile phase:
Flow rate:
100L/min.
Detector:
(Micro flow-thru cell was used.)
Shim-pack
Shim-pack Ion Exchange Columns
The
Shim-pack ion exchange columns are available in various types as shown below.
Shim-pack WAX-1
used for analysis of nucleotides
and oligonucleotides
Anion exchange
Silica gel type
Shim-pack
ion exchange columns
Shim-pack WAX-2
used for analysis of proteins
Chemically-bonded
hydrophilic silica gel
(Hydrophilic gel)
Cation exchange
Shim-pack WCX-1
Anion exchange
Shim-pack PA-DEAE, PA-QA

and nucleic acids
Cation exchange
Shim-pack PA-CM, PA-SP

and nucleic acids
Anion exchange
Shim-pack ISA
used for analysis of sugars
Cation exchange
Shim-pack ISC
used for analysis of amino acids
and guanidino compounds
Polyacrylate
Polymer type
(Hydrophobic gel)
Polystyrene
Shim-pack
The
ion exchange column series are classified into silica gel type and polymer type.
silica gel type columns ensure higher number of theoretical plates than the polymer type, because they use 5m or 3m
diameter packing materials in contrast to 10m diameter packing materials used in the latter.
The
polymer type columns ensure better pH stability than the silica gel type and so they are applicable to wider pH range
mobile phases.
Polymer
types are classified into polyacrylate type having hydrophilic gel and polystyrene type having hydrophobic gel.
Therefore it is possible to use each depending on objecting samples.

The
Shim-pack WAX/WCX series columns are suitable for analysis of nucleotides, oligonucleotides, and proteins.
The
Shim-pack PA series columns use hydrophilic polymers as the solid support. They are especially suitable for the
preparative LC of biological substances such as proteins and nucleic acids. The columns are available in two diameters,
8mm i.d. for determining the preparation conditions and 20mm i.d. for preparative work.
The
Shim-pack ISA/ISC series columns, which use polystyrene gel as the solid support, utilize electrostatic reaction and
hydrophobic reaction. These columns are suitable for the analysis of sugars (ISA), amino acids (ISC-07), and guanidiono
compounds (ISC-05).
33
Shim-pack WAX, WCX: Silica Gel Type Ion Exchange Column

Column name
Stationary phase
Particle dia. (m)
Dimensions
Cat. No.
Shim-pack WAX-1
Tertiary amino group
4.0mm i.d.5cm
228-16225-91
Shim-pack WAX-2
4.0mm i.d.5cm
228-16365-91
Shim-pack WCX-1
Corboxyl group
4.0mm i.d.5cm
228-16366-91
It is necessary to connect a precolumn (Cat. No. 228-16367-91) between the liquid pump and the sample injector.
Shim-pack PA: Polyacrylate Type Ion Exchange Column

Column name
Shim-pack PA-DEAE
Shim-pack PA-QA
Shim-pack PA-CM
Shim-pack PA-SP
Stationary phase
Particle dia. (m)
Diethyl amino ethyl group
10
Tetramethyl ammonium group
10
Carboxy methyl group
10
Surforpropyl group
10
Dimensions
Cat. No.
8.0mm i.d.10cm
228-20758-91
20mm i.d.10cm
228-20758-92
8.0mm i.d.10cm
228-20759-91
20mm i.d.10cm
228-20759-92
8.0mm i.d.10cm
228-20760-91
20mm i.d.10cm
228-20760-92
8.0mm i.d.10cm
228-20761-91
20mm i.d.10cm
228-20761-92
Dimensions
Cat. No.
Shim-pack PAG: Guard Column for Shim-pack PA

Column name
Shim-pack PAG-DEAE
Stationary phase
Particle dia. (m)
Diethyl amino ethyl group
10
8.0mm i.d.1cm
228-20762-91
Shim-pack PAG-QA
10
8.0mm i.d.1cm
228-20763-91
Shim-pack PAG-CM
Carboxy methyl group
10
8.0mm i.d.1cm
228-20764-91
Shim-pack PAG-SP
Surforpropyl group
10
8.0mm i.d.1cm
228-20765-91
The Shim-pack PAG columns are also used for analysis. (See Fig. 46.)
Shim-pack ISC, ISA: Polystyrene Type Ion Exchange Column

Column name
Stationary phase
Particle dia. (m)
Dimensions
Cat. No.
Na type sulfone group
6.0mm i.d.10cm
228-18837-91
Shim-pack Amino-Li
Li type sulfone group
6.0mm i.d.10cm
228-18337-92
Shim-pack ISC-05/S0504
4.6mm i.d.3.8cm
228-09700-91
4.0mm i.d.15cm
228-09328-91
Shim-pack Amino-Na
Shim-pack ISC-07/S1504Li
4.0mm i.d.15cm
228-00796-91
Quaternery ammonium group
4.0mm i.d.25cm
228-09699-91
Ammonia Trap Column

Column name
Dimensions
Cat. No.
Shim-pack ISC-30/S0504 (For trapping Na type ammonia)
4.0mm i.d.5cm
228-14206-91
Shim-pack ISC-30/S0504Li (For trapping Li type ammonia)
4.0mm i.d.5cm
228-00821-91
Dimensions
Cat. No.
It is necessary to use one of the columns, in analysis of amino acids.
Guard Column
The following are dedicated guard columns.
Column name
34
ISA guard column
4.0mm i.d.5cm
228-00823-91
ISC-07 guard column (Na type)
4.0mm i.d.5cm
228-00802-91
ISC-07 guard column (Li type)
4.0mm i.d.5cm
228-00797-91
Shim-pack
Shim-pack WCX
Peaks
. Hemoglobin F
. Hemoglobin A
. Hemoglobin S
. Hemoglobin C
Peaks
. Ovalbumin
. Myoglobin
. -Chymotrypsinogen A
. Ribonuclease A
. Lysozyme
Fig. 38
Analysis of Protein Standard
Column:
Shim-pack WCX-1 (4.0mm i.d.5cm)
Mobile phase:
20mM phosphate buffer solution (pH 6.0)/sodium
sulfate, gradient elution
Flow rate:
1.0mL/min.
Detector:
UV spectrophotometric detector
Fig. 39
Fig. 40
Urease
Analysis of Hemoglobins
Column:
Mobile phase:
Flow rate:
1.0mL/min.
Detector:
Determination of Urease
Column:
Mobile phase:
Flow rate:
1.0mL/min.
Detector:
Shim-pack WAX
Peaks
. UMP
. CMP
. AMP
. GMP
. UDP
. CDP
. ADP
. UTP
. GDP
10. CTP
11. ATP
12. GTP
Poly (dT)
Fig. 41
Analysis of Synthesized DNAs
Column:
Shim-pack WAX-1 (4.0mm i.d.5cm)
Mobile phase:
Phosphate buffer solution (pH 6.8)/acetonitrile,
gradient elution
Flow rate:
1.0mL/min.
Detector:
Fig. 42
Analysis of Mononucleotides
Column:
Mobile phase:
20mM phosphate buffer solution (pH 7)/480mM
phosphoric acid buffer solution (pH 6.85), gradient
elution
Flow rate:
1.0mL/min.
Detector:
35
Peaks
. Hemoglobin S
. Hemoglobin A
. Hemoglobin F
Peaks
. Cytochrome C
. Ovalbumin
. Trypsin inhibitor
Fig. 43
Column:
Mobile phase:
Tris-sulfuric acid buffer solution (pH 7.5)/sodium
Flow rate:
1.0mL/min.
Detector:
Fig. 44
Analysis of Hemoglobins
Column:
Mobile phase:
Tris-acetic acid buffer solution (pH 8) /sodium
Flow rate:
1.0mL/min.
Detector:
UV-VIS spectrophotometric detector (415nm)
Shim-pack PA
Peaks
. Myoglobin
. Ribonuclease A
. Lysozyme
Peaks
. Carbonic anhydrase
. Conalbumin
. -Lactalbumin
. Bovine scrum albumin
. -Lactoglobulin B
. -Lactoglobulin A
Fig. 45
Column:
Shim-pack PA-DEAE (8.0mm i.d.1cm)
Mobile phase:
Tris-hydrochloric acid buffer solution (pH 8.0)
/sodium chloride, gradient elution
Flow rate:
1.0mL/min.
Detector:
Fig. 46
Peaks
. Myoglobin
. Ribonuclease A
. Lysozyme
Peaks
. Myoglobin
. Ribonuclease A
. Lysozyme
Fig. 47
Column:
Shim-pack PA-SP (8.0mm i.d.1cm)
Mobile phase:
Phosphate buffer solution (pH 6.6) /sodium
chloride, gradient elution
Flow rate:
1.0mL/min.
Detector:
36
Column:
Shim-pack PAG-CM (8.0mm i.d.1cm)
Mobile phase:
Flow rate:
1.0mL/min.
Detector:
Fig. 48
Column:
Shim-pack PA-CM (8.0mm i.d.1cm)
Mobile phase:
Flow rate:
1.0mL/min.
Detector:
Shim-pack
Shim-pack ISA, ISC

Peaks
. Sucrose
. Cellobiose
. Maltose
. Lactose
. Rhamnose
. Ribose
. Mannose
. Fructose
. Galactose
10. Xylose
11. Giucose
Fig. 49
Peaks
. Asp
. Thy
. Ser
. Glu
. Pro
. Gly
. Ala
. Cystine
. Val
Fig. 50
Analysis of Saccharide Standard
Column:
Shim-pack ISA-07/S2504 (4.0mm i.d.25cm)
Mobile phase:
Potassium borate buffer solution, gradient elution
Flow rate:
0.6mL/min.
Spectrofluorophotometric detector (EX. 348nm, Em. 430nm)
Detector:
Method:
Post-column derivatization with arginine
Met
Ile
Leu
Tyr
Phe
His
Lys
Arg
Analysis of Amino Acid Standard
Column:
Shim-pack ISC-07/S1504 (4.0mm i.d.15cm)
Mobile phase:
Sodium citrate buffer solution, gradient elution
Flow rate:
0.3mL/min.
Spectrofluorophotometric detector (EX. 348nm, Em. 450nm)
Detector:
Method:
Post-column derivatization with ortho-phthalaldehyde
Peaks
. p-Ser
. Tau
. p-EtNH2
. Asp
. Hypro
. Thy
. Ser
. Asn
. Glu
10. Gln
11. Sar
12. -AAA
13. Pro
14. Gly
15. Ala
16. Cit
17. -AnBA
18. Val
19. Met
Fig. 51
10.
11.
12.
13.
14.
15.
16.
17.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
34.
35.
36.
37.
Cystine
Ile
Leu
Tyr
Pre
-Ala
-AiBA
-ABA
His
3-Me-His
1-Me-His
Car
Ans
HyLys
Orn
Lys
NH3+EtNH2
Arg
Analysis of Biological Amino Acids
Column:
Shim-pack ISC-07/S1504Li (4.0mm i.d.15cm)
Mobile phase:
Lithium citrate buffer solution, gradient elution
Flow rate:
0.4mL/min.
Detector:
Spectrofluorophotometric detector
(EX. 348nm, Em. 450nm)
Post-column derivatization with ortho-phthalaldehyde
Method:
37
Shim-pack GPC
Shim-pack
GPC Series columns are used for the determination of analysis of organic solvent solution of tetrahydrofuran
(800 Series), determination of chloroform (800C Series), and determination of dimethylformamide (800D series).
The technique of GPC does not utilize such chemical reactions as partition, adsorption, and ion exchange, but a physical
reaction, that is , separation based on molecular size of the sample components. Therefore, this method is suitable for the
measurement of molecular weight distribution of high polymers and oligomers.
Shim-pack GPC series columns are packed with polystyrene polymers of respective degree of cross-linking, so that you
can choose a column that exactly meets your requirements, ranging from analysis of high polymers to that of oligomers.
GPC-80M (80MC, 80MD) are mixed gel columns which get the straight of calibration curve in wide range of molecular
weight.
Shim-pack GPC-2000 Series are dedicated to preparative LC of tetrahydrofuran and chloroform. They ensure separation
efficiency comparable to analytical columns as well as large preparative capacity.
Shim-pack GPC (dedicated to determination of tetrahydrofuran)

Column name
Exclusion limit (polystyrene)
Dimensions
Cat. No.
Shim-pack GPC-801
1.510
8.0mm i.d.30cm
228-20803-91
Shim-pack GPC-802
5103
8.0mm i.d.30cm
228-20804-91
Shim-pack GPC-8025
2104
8.0mm i.d.30cm
228-20805-91
Shim-pack GPC-803
7104
8.0mm i.d.30cm
228-20806-91
Shim-pack GPC-804
4105
8.0mm i.d.30cm
228-20807-91
Shim-pack GPC-805
410
8.0mm i.d.30cm
228-20808-91
Shim-pack GPC-806
4107
8.0mm i.d.30cm
228-20809-91
Shim-pack GPC-80M
4107, Mixed gel
8.0mm i.d.30cm
228-20810-91
Shim-pack GPC-807
2108
8.0mm i.d.30cm
228-20811-91
Guard Column (for the above)

Column name
Dimensions
Cat. No.
Shim-pack GPC-800P
4.6mm i.d.1cm
228-20812-91
Shim-pack GPC (dedicated to determination of chloroform)

Column name
Dimensions
Cat. No.
Shim-pack GPC-801C
1.5103
8.0mm i.d.30cm
228-20803-92
Shim-pack GPC-802C
5103
8.0mm i.d.30cm
228-20804-92
Shim-pack GPC-8025C
2104
8.0mm i.d.30cm
228-20805-92
Shim-pack GPC-803C
710
8.0mm i.d.30cm
228-20806-92
Shim-pack GPC-804C
4105
8.0mm i.d.30cm
228-20807-92
Shim-pack GPC-805C
4106
8.0mm i.d.30cm
228-20808-92
Shim-pack GPC-806C
4107
8.0mm i.d.30cm
228-20809-92
Shim-pack GPC-80MC
4107, Mixed gel
8.0mm i.d.30cm
228-20810-92
Shim-pack GPC-807C
210
8.0mm i.d.30cm
228-20811-92
38
Column name
Dimensions
Cat. No.
Shim-pack GPC-800CP
4.6mm i.d.1cm
228-20812-92
Shim-pack
Shim-pack GPC (dedicated to determination of dimethylformamide)

Column name
Dimensions
Cat. No.
Shim-pack GPC-801D
1.5103
8.0mm i.d.30cm
228-20803-93
Shim-pack GPC-802D
5103
8.0mm i.d.30cm
228-20804-93
Shim-pack GPC-8025D
2104
8.0mm i.d.30cm
228-20805-93
Shim-pack GPC-803D
7104
8.0mm i.d.30cm
228-20806-93
Shim-pack GPC-804D
410
8.0mm i.d.30cm
228-20807-93
Shim-pack GPC-805D
4106
8.0mm i.d.30cm
228-20808-93
Shim-pack GPC-806D
4107
8.0mm i.d.30cm
228-20809-93
Shim-pack GPC-80MD
4107, Mixed gel
8.0mm i.d.30cm
228-20810-93
Shim-pack GPC-807D
2108
8.0mm i.d.30cm
228-20811-93

Column name
Dimensions
Cat. No.
Shim-pack GPC-800DP
4.6mm i.d.1cm
228-20812-93
Shim-pack GPC-2000 Series

Dimensions
Cat. No.
Shim-pack GPC-2001*
Column name
1.5103
20mm i.d.30cm
228-23342-91
Shim-pack GPC-2002*
5103
20mm i.d.30cm
228-23342-92
Shim-pack GPC-20025*
2104
20mm i.d.30cm
228-23342-93
Shim-pack GPC-2003*
7104
20mm i.d.30cm
228-23342-94
Shim-pack GPC-2001C**
1.5103
20mm i.d.30cm
228-23343-91
5103
20mm i.d.30cm
228-23343-92
210
20mm i.d.30cm
228-23343-93
7104
20mm i.d.30cm
228-23343-94
*For preparative LC of tetrahydrofuran.
**For preparative LC of chloroform.

Column name
Dimensions
Cat. No.
Shim-pack GPC-2000P (For preparative LC of tetrahydrofuran.)
8mm5cm
228-20812-94
Shim-pack GPC-2000CP (For preparative LC of chloroform.)
8mm5cm
228-20812-95
Shim-pack GPC
Peaks
. Polystyrene M.w. 9,120K
. Polystyrene
1,100K
. Polystyrene
410K
. Polystyrene
115K
. Polystyrene
33K
. Polystyrene
7.6K
. Polystyrene
1.35K
. n-Propylbenzene
Fig. 52
Calibration Curves
Mobile phase:
Tetrahydrofuran
Flow rate:
1.0mL/min.
Detector:
Fig. 53
Analysis of Polystyrene Standard
Column:
Shim-pack GPC-80M (2 columns in series)
Mobile phase:
Tetrahydrofuran
Flow rate:
1.0mL/min.
Detector:
39
Peaks
. Polystyrene Mw 42,800
. Polystyrene Mw 2,800
. Polystyrene Mw
300
. n-Propylbenzene
. Benzene
Fig. 54
Analysis of Polystyrene Standard
Column:
Shim-pack GPC-802
Mobile phase:
Tetrahydrofuran
Flow rate:
1.0mL/min.
Detector:
Fig. 56
Analysis of Polymethylbenzene
Column:
Shim-pack GPC-80MC (2pcs.) (8mm i.d.300mm)
Mobile phase:
Chloroform/Trichlorotrifluoroethane (8/2)
Flow rate:
1.0mL/min.
Detector:
Fig. 58
Analysis of Vinyleden Poly fluoride
Column:
Shim-pack GPC-80MD (8mm i.d.300mm)
Mobile phase:
DMF (including 10mM LiCL)
Flow rate:
0.8mL/min.
Detector:
40
Fig. 55
Analysis of Epoxy Resin
Column:
Shim-pack GPC-803 (4 pcs.) and Shim-pack GPC8025 (4 pcs.)
Mobile phase:
Tetrahydrofuran
Flow rate:
1.0mL/min.
Detector:
Fig. 57
Analysis of Polythioethersulfone
Column:
Shim-pack GPC-80MC (8mm i.d.300mm)
Mobile phase:
Chloroform
Flow rate:
0.5mL/min.
Detector:
Fig. 59
Analysis of Polyetherimide
Column:
Shim-pack GPC-80MD (2 pcs.) (8mm i.d.300mm)
Mobile phase:
DMF (including 10mM LiCL)
Flow rate:
1.0mL/min.
Detector:
Shim-pack
Shim-pack Columns for Gel Filtration / Ion Exclusion Chromatography
Gel
filtration chromatography (GFC) is used to separate water-soluble high polymers such as polysaccharides, proteins,
and nucleic acids, according to the molecular sizes.

Shimadzu
HPLC columns for gel filtration chromatography and ion exclusion chromatography may be classified as follows.
Silica type
Shim-pack Diol
Proteins and water-soluble high

polymers
Shim-pack SCR
Saccharides(Separation is made
by gel filtration and ligand exchange)
Gel filtration Chromatography Columns

Sulfonated polystyrene
(Except SCR-101H/102H)
Polymer type
Polyvinyl alcohol
Asahipak GS, GFA
Protein, peptides, nucleic acids,

saccharides
Shim-pack
Ion Exclusion Chromatography Columns
Polymer type
Sulfonated polystyrene
SCR-101H
Organic acids
SCR-102H
Analysis
of polysaccharides and oligosaccharidesAsahipak GS series are recommended.
Analysis
of monosaccharidesShim-pack SCR series is recommended. Since the separation

is made by a combination of gel filtration and ligand exchange,
the selectivity differs with the type of cation.
Analysis
of proteins Shim-pack Diol series, and Asahipak GS series are

recommended. The silica type provides sharper peaks while the
polymer type ensures better alkali resistancy.
Analysis
of organic acids Ion exclusion chromatography using Shim-pack SCR-101H and

SCR-102H is recommended. An acidic aqueous solution (e.g.
aqueous solution of perchloric acid) is used as mobile phase
and the H type sulfonated styrene polymer as stationary phase.
41
Shim-pack SCR
Column name
Stationary phase
Particle dia. (m)
Dimensions
Cat. No.
Shim-pack SCR-101N
10
7.9mm i.d.30cm
228-07730-92
Shim-pack SCR-101C
Ca type sulfone group
10
7.9mm i.d.30cm
228-17889-91
Shim-pack SCR-101P
Pb type sulfone group
10
7.9mm i.d.30cm
228-17890-91
Shim-pack SCR-101H
H type sulfone group
10
7.9mm i.d.30cm
228-07730-93
Shim-pack SCR-102H
8.0mm i.d.30cm
228-17893-91
Guard Column for Shim-pack SCR

Column name
Dimensions
Cat. No.
Guard column SCR(N) (Dedicated to SCR-101N)
4.0mm i.d.5cm
228-09619-92
Guard column SCR(H) (Dedicated to SCR-101H)
4.0mm i.d.5cm
228-09619-93
Guard column SCR(C) (Dedicated to SCR-101C)
4.0mm i.d.5cm
228-17891-91
Guard column SCR(P) (Dedicated to SCR-101P)
4.0mm i.d.5cm
228-17892-91
Guard column SCR-102H (Dedicated to SCR-102H)
6.0mm i.d.5cm
228-17924-91
Use a guard column without fail.
Shim-pack Diol
Column name
Stationary phase
Particle dia. (m)
Shim-pack Diol-150
Diol group
Shim-pack Diol-300
Diol group
Dimensions
Cat. No.
7.9mm i.d.25cm
228-14775-91
7.9mm i.d.50cm
228-14775-92
7.9mm i.d.25cm
228-14776-91
7.9mm i.d.50cm
228-14776-92
Install a precolumn (Cat. No. 228-16367-91) between the liquid pump and the sample injector.
Shim-pack ION KS
Sample
Saccharides
Fig. 60 Calibration Curves for ION KS Series Columns

Column:
Shim-pack ION KS (8.0mm i.d.30cm)
Mobile phase:
Water
Flow rate:
1.0mL/min.
Detector:
42
Shim-pack
Shim-pack SCR
Peaks
. PEG 4000
. Maltose
. Glucose
. Fructose
. Glycerol
. Ethanol
. Sorbitol
Peaks
. Polysaccharides
. Moltotriose
. Maltose
. Glucose
Fig. 61
Analysis of Dextrin
Fig. 62
Column:
Shim-pack SCR-101N (7.9mm i.d.30cm)
Mobile phase:
Water
Flow rate:
0.8mL/min.
Detector:
Column:
Shim-pack SCR-101C (7.9mm i.d.30cm)
Mobile phase:
Water
Flow rate:
1.0mL/min.
Detector:
Peaks
. Sucrose
. Glucose
. Xylose
. Galactose
. Arabinose
. Glycerol
. Mannitol
. Sorbitol
Fig. 63
Column:
Shim-pack SCR-101p (7.9mm i.d.30cm)
Mobile phase:
Water
Flow rate:
0.6mL/min.
Detector:
Peaks
. Phosphoric acid
. -Ketoglutaric acid
. Citric acid
. Pyruvic acid
. Malic acid
. Succinio acid
. Lactic acid
. Formic acid
. Fumaric acid
10. Acetic acid
11. Levulinic acid
12. Pyroglutamic acid
Fig. 64
Analysis of Organic Acids
Column:
Shim-pack SCR-102H (7.9mm i.d.30cm) (2 pcs.)
Mobile phase:
5mM p-Toluene sulfonic acids aqueous solution
Flow rate:
0.8mL/min.
Detector:
Conductivity detector (pH buffer organic acids
analysis system)
43
Shim-pack Diol
Peaks
. Blue dextran 2000
. Thyroglobulin
. y-Globulin
. Transferrin
. Human serum albumin
. Ovalbumin
. -Lactoglobulin
. Chymotrypsin
. Myoglobin
10. Lysozyme
11. Ribonuclease A
12. Cytochrome C
13. Gly-Tyr
Fig. 65 Calibration Curve for Shim-pack Diol Columns

Column:
Shim-pack Diol (7.9mm i.d.50cm)
Mobile phase:
10mM phosphate buffer solution (pH 7) and 0.2M
sodium sulfate
Flow rate:
1.0mL/min.
Detector:
Peaks
. Glutamate dehydrogenase
. Lactate dehydrogenase
. Enolase
. Adenylate kinase
. Cytochrome C
Albumin
IgG
Fig. 66
Column:
Shim-pack Diol-300 (7.9mm i.d.50cm)
Mobile phase:
sodium chloride
Flow rate:
1.0mL/min.
Detector:
Fig. 67
Analysis of Human Serum
Column:
Mobile phase:
sodium sulfate
Flow rate:
1.0mL/min.
Detector:
Peaks
. PEG 6000
. PEG 4000
. PEG 1000
. Ethyleneglycol
Ovalbumin
Lysozyme
Fig. 68
Analysis of Albumin
Column:
Mobile phase:
sodium sulfate
Flow rate:
1.0mL/min.
Detector:
44
Fig. 69
Analysis of Polyethylene Glycol
Column:
Mobile phase:
Water
Flow rate:
1.0mL/min.
Detector:
Shim-pack
Shim-pack IC
Shim-pack
The
IC series is ion exchange columns developed for ion chromatography.
Shim-pack IC series columns are classified as follows.
Shim-pack IC-C1
Used for analysis of cations such as alkaline metal ions, alkali

earth metal ions, transition metal ions, rare earth metal ions,
and alkyl amines.
Shim-pack IC-C3
Used for simultaneous analysis of alkaline metal ions and alkali

earth metal ions. Also, suitable for separation of ethanol amines
and alkyl amines.
Shim-pack IC-A1
Used for analysis of anions including inorganic anions and

organic ions. Compatible with mobile phases of wide pH range,
acidic or basic.
Shim-pack IC-A2
Low ion exchange type and possible to make the concentration

of salt in mobile phase low and the background reduce more.
But use only in the condition of an acid.
Shim-pack IC-A3
Provides outstanding separating efficiency in analysis of

inorganic anions acid ions, especially when weakly acidic mobile
phase is used.
Cation Analysis
Shim-pack
IC series
Anion Analysis
Shim-pack IC
Stationary phase
Particle dia. (m)
Dimensions
Cat. No.
Shim-pack IC-A1
Column name
Quaternary ammonium group
12.5
4.6mm i.d.10cm
228-17733-91
Shim-pack IC-A1(S)
10
2.0mm i.d.15cm
228-33400-91
Shim-pack IC-A2
10
4.6mm i.d.5cm
228-17735-91
Shim-pack IC-A3
4.6mm i.d.15cm
228-31076-91
Shim-pack IC-A3(S)
2.0mm i.d.15cm
228-33366-91
Shim-pack IC-C1
Sulfone group
10
5.0mm i.d.15cm
228-17737-91
Shim-pack IC-C1 PEEK
Sulfone group
10
4.6mm i.d.10cm
228-33497-91
Shim-pack IC-C3
Carboxylic group
4.6mm i.d.10cm
228-32329-91
Shim-pack IC-C3(S)
Carboxylic group
2.0mm i.d.10cm
228-33367-91
*Shim-pack IC(S) series are for semi-micro LC. The use of this with PIA-1000 is recommended.
Shim-pack IC-G
Column name
Dimensions
Cat. No.
Shim-pack IC-GA1 (Dedicated to IC-A1)
4.6mm i.d.1cm
228-17734-91
4.0mm i.d.1cm
228-17736-91
4.6mm i.d.1cm
228-31076-92
Shim-pack IC-GC1 (Dedicated to IC-C1)
4.0mm i.d.1cm
228-17738-91
Shim-pack IC-GC1 PEEK (Dedicated to IC-C1 PEEK)
4.6mm i.d.1cm
228-33497-92
Shim-pack IC-GC3 (Dedicated to IC-C3)
4.6mm i.d.7.5mm
228-41176-91
Precolumn
Column name
Dimensions
Cat. No.
Shim-pack IC-PC1 (Dedicated to IC-C1)
8.0mm i.d.5cm
228-17744-91
In analysis of alkali metal ions with the Shim-pack IC-C1, the use of the pre-column is recommended.
45
Shim-pack IC
Peaks
. PO43
. F
. CR
. NO2
. Br
. NO3
. SO42
Fig. 70 Analysis of Inorganic Anions
Peaks
. Li
. Na
. NH4
. K
. Rb
. Cs
Fig. 71
Column:
Shim-pack IC-A3 (4.6mm i.d.15cm)
8.0mM p-Hydroxybenzoic acid and 3.2 mM Bis-Tris
Mobile phase:
Flow rate:
1.5mL/min.
Detector:
Conductivity detector
Bis(2-hydroxyethyl) iminotris (hydroxymethyl) methane
Analysis of Alkali Metal Ions
Column:
Shim-pack IC-C1 (5.0mm i.d.15cm)
Mobile phase:
5mM nitric acid
Flow rate:
1.5mL/min.
Detector:
Peaks
. Na
. NH4
. K
. Mg2
. Ca2
Fig. 72
Analysis of Cations in Water (includes ammonia)
Column:
Shim-pack IC-C3 (4.6mm i.d.10cm)
Mobile phase:
2.5mM oxalic acid
Flow rate:
1.0mL/min.
Detector:
46
Peaks
. Fe3
. Cu2
. Zn2
. Ni2
. Co2
. Fe2
. Cd2
. Mn2
Fig. 73
Analysis of Transition Metal Ions
Column:
Shim-pack IC-C1 (5mm i.d.15cm)
Mobile phase:
0.3M lactic acid
Flow rate:
1.0mL/min.
Detector:
UVVIS spectrophotometric detector
Method:
Post-column derivatization with 4- (2-pyridilazo
resorcinol)
Shim-pack
Shim-pack HPC
Shim-pack
HPC columns have been specifically developed for the hydrophobic LC of biological high polymers such as
proteins and nucleic acids.

The
columns are packed with fully-porous spherical particles, 5m in diameter and 300 in pore diameter. The effect of
the residual silanol groups is minimized by the unique chemical treatment technique.
Ethyl
groups or n-propyl groups are chemically bonded as stationary phase.
Hydrophobic LC and Reversed Phase LC

Both of these techniques use hydrophobic column packings, but are different in the following points.
The
In
column packings for reversed phase LC are more hydrophobic than those for hydrophobic LC.
reversed phase LC, since organic solvent is generally used as mobile phase, the high-degree structures of proteins are often
transmuted and the enzymes deactivated. In hydrophobic LC, on the other hand, since buffer solutions containing some salt are
generally used, the high-degree structures of proteins are hardly transmuted.
The
retention of sample components is more dependent on the degree of hydrophobicity of the stationary phase in hydrophobic LC
than in reversed phase LC. It is, therefore, more important to select the stationary phase, HPC-C2 or HPC-C3, in hydrophobic LC.
Shim-pack HPC
Column name
Stationary phase
Particle dia. (m)
Dimensions
Cat. No.
Shim-pack HPC-C2
Ethyl group
4.0mm i.d.5cm
228-17792-91
Shim-pack HPC-C3
n-Propyl group
4.0mm i.d.5cm
228-17793-91
*It is necessary to install a pre-column between the liquid pump and the sample injector, to protect the Shim-pack HPC column.
47
Shim-pack HPC
Peaks
. Cytochrome C
. Myoglobin
. Ribonuclease A
. Transferrin
Fig. 74
Column:
Shim-pack HPC-C2 (4.0mm i.d.5cm)
Ammonium sulfate/phosphate buffer solution (pH 7),
Mobile phase:
gradient elution
Flow rate:
0.5mL/min.
Detector:
Fig. 75
Analysis of Snake Venom
Column:
Mobile phase:
gradient elution
Flow rate:
0.5mL/min.
Detector:
Peaks
. Cytochrome C
. Myoglobin
. Lysozyme
. Albumin
Fig. 76 Analysis of Glucosidase

Column:
Mobile phase:
gradient elution
Flow rate:
0.5mL/min.
Detector:
48
Fig. 77
Analysis of Ribonucleic Acids
Column:
Mobile phase:
gradient elution
Flow rate:
0.5mL/min.
Detector:
Shim-pack
Shim-pack SPC
The
Shim-pack SPC has specifically developed for automatic sample pretreatment and concentration by the column
switching method.
The
Shim-pack SPC-RP3 column is packed with polymer particles and used for reversed phase LC.
The
Shim-pack SPC-AE1 column is packed with fully porous silica gel particles on which weakly basic anion exchange
functions are chemically bonded.

The
Shimadzu Automatic Sample Pretreatment HPLC System is recommended.
HPLC System with Automatic Sample Pretreatment Functions

This system is used for analysis of samples that contain many interfering compounds. In the first step, the sample is roughly separated in
the pretreating column. Then, only the fraction that contains the target compounds is made to enter the analytical column, the switching
being done with a valve. The sample pretreatment is carried out in a completely automated sequence.
Fig. 78
Shimadzu Automatic Sample Pretreatment HPLC System
Shim-pack SPC
Column name
Shim-pack SPC-RP3
Shim-pack SPC-RP2
Shim-pack SPC-AE1
Stationary phase
Polymer
Polymer
Particle dia. (m)

9
10
10
Separation mode
Reversed phase
Reversed phase
Anion exchange
Dimensions
4.0mm i.d.3cm
4.6mm i.d.1cm
4.0mm i.d.1cm
Cat. No.
228-33713-91
228-18838-91
228-17990-91
49
Shim-pack GRD, Pre-column

Undissolved
materials in mobile phase is the cause of stuffing analytical column and consuming packing materials.
Installing Shim-pack GRD-ODS, you can escape from these causes above. Connect this between the liquid pump and the
sample injector bellow.
Using
GRD, Pre-column makes the life of analytical columns long.
Shim-pack GRD-ODS
Effective particuraly when reversed phase column is used and both mobile phase pH is higher than neutral and ion pair reagent involving
ritrogen such as tetrabutyl ammonium is used.
Pre-column Diol
The use of pre-column is recommended when using Shim-pack Diol, Shim-pack WAX, WCX, Shim-pack HPC series. Particurely when
the concentration of the salt is high, it is available to eliminate un-dissolved materials in salt.
1. Mobile phase
2. Liquid Pump
3. Precolumn
4. (In case of filter GRD)
5. Sample injector
6. Analytical column
7. Oven
8. Detector
9. Drain
Fig. 79
Flow line having Pre-column
Shim-pack GRD, Pre-column (It is necessary to install a pre-column between the liquid pump and the sample injector.)
Column name
Stationary phase
Particle dia. (m)
Dimensions
Cat. No.
Shim-pack GRD-ODS
Octadecyl group
3050
4.0mm i.d.25cm
228-16557-91
Diol group
10
4.0mm i.d.5cm
228-16367-91
Pre-column Diol
Packing materials of GRD-ODS
50
Column name
Stationary phase
Particle dia. (m)
Weight
Cat. No.
Gel GRD-ODS
Octadecyl group
3050
10g
228-16831-91
Shim-pack
Dedicated Shim-pack Columns
The
Shim-pack Series columns may be classified as follows, according to the applicable type of samples.
Protein composing amino acids
Amino acid analysis

Biological amino acids
Gel filtration
Shim-pack ION series
Gel filtration and ligand exchange
Shim-pack SCR series
Saccharide analysis
Organic acid analysis
Ion exclusion
Shim-pack SCR-101H, 102H
HVA/VMA analysis
Shim-pack CLC-VMA
Hop acid analysis
Shim-pack CLC-ODS/H
Applications of the Shim-pack CLC-VMA column and the Shim-pack CLC-ODS/H column
Peaks
. DOMA
. MHPG
. VMA
. DOPAC
. VLA
. HVA
Peaks
. cis-and trans-lsocohumulone
. cis-and trans-lsohumulone
. cis-and trans-lsoadhumulone
. -Phenylchalkone (I.S.)
Fig. 80 Analysis of Hop Acids

Column:
Shim-pack CLC-ODS/H (4.6mm i.d.25cm)
Mobile phase:
Methanol/water/phosphoric acid/10% tetra ethyl
ammonium (77.5/22.5/1.1/3)
Flow rate:
1.5mL/min.
Detector:
Fig. 81
Analysis of Catecholamine Metabolites
Column:
Shim-pack CLC-VMA (6.0mm i.d.15cm)
Mobile phase:
3mM tartaric acid/acetonitrile (7/3)
Flow rate:
1.0mL/min.
Fluorescence HPLC monitor (Ex. 280nm, Em. 320nm)
Detector:
51
Columns for Amino Acid Analysis

Column name
Stationary phase
Particle dia. (m)
Dimensions
Cat. No.
6.0mm i.d.10cm
228-18837-91
Shim-pack Amino-Li
6.0mm i.d.10cm
228-18837-92
Shim-pack ISC-07/S1504Na
4.0mm i.d.15cm
228-09328-91
4.0mm i.d.15cm
228-00796-91
Column name
Dimensions
Cat. No.
ISC-07 guard column (Na type)
4.0mm i.d.5cm
228-00802-91
ISC-07 guard column (Li type)
4.0mm i.d.5cm
228-00797-91
Shim-pack Amino-Na
The following guard columns are available for amino acid analysis.
The following column must be used in amino acid analysis.
Column name
Dimensions
Cat. No.
Shim-pack ISC-30/S0504Na (for trapping Na type ammonia)
4.0mm i.d.5cm
228-14206-91
Shim-pack ISC-30/S0504Li (for trapping Li type ammonia)
4.0mm i.d.5cm
228-00821-91
Columns for Saccharide Analysis

Column name
Exclusion limit (dextran)
Dimensions
Cat. No.
Shim-pack ION KS-801
1103
8.0mm i.d.30cm
228-17894-91
1104
8.0mm i.d.30cm
228-17895-91
5104
8.0mm i.d.30cm
228-17896-91
4105
8.0mm i.d.30cm
228-17897-91
The following dedicated guard column is available
Column name
Dimensions
Cat. No.
Shim-pack ION KS-800P (Common to ION KS series)
6.0mm i.d.5cm
228-17898-91
Column name
Stationary phase
Particle dia. (m)
Dimensions
Cat. No.
Shim-pack SCR-101N
10
7.9mm i.d.30cm
228-07730-92
Shim-pack SCR-101C
Ca type sulfone group
10
7.9mm i.d.30cm
228-17889-91
Shim-pack SCR-101P
Pb type sulfone group
10
7.9mm i.d.30cm
228-17890-91
Column name
Dimensions
Cat. No.
SCR(N) guard column, dedicated to SCR-101N
4.0mm i.d.5cm
228-09619-92
SCR(C) guard column, dedicated to SCR-101C
4.0mm i.d.5cm
228-17891-91
SCR(P) guard column, dedicated to SCR-101P
4.0mm i.d.5cm
228-17892-91
The following guard column must be used.
Columns for Organic Acid Analysis

Column name
Stationary phase
Particle dia. (m)
Dimensions
Cat. No.
Shim-pack SCR-101H
10
7.9mm i.d.30cm
228-07730-93
Shim-pack SCR-102H
8.0mm i.d.30cm
228-17893-91
Column name
Dimensions
Cat. No.
SCR(H) guard column, dedicated to SCR-101H
4.0mm i.d.5cm
228-09619-93
SCR-102H guard column, dedicated to SCR-102H
6.0mm i.d.5cm
228-17924-91
Columns for VMA/HVA Analysis

Column name
Shim-pack CLC-VMA
Stationary phase
Polar group
Particle dia. (m)

5
Separation mode
Reversed
Dimensions
Cat. No.
6.0mm i.d.15cm 228-17255-91
Particle dia. (m)

5
Separation mode
Reversed
Dimensions
Cat. No.
4.6mm i.d.25cm 228-00808-92
Columns for Hop Acid Analysis

Column name
Shim-pack CLC-ODS/H
52
Stationary phase
Octadecyl group
Shim-pack
Shim-pack Preparative Columns
The
Shim-pack preparative columns are available as follows.
Shim-pack PRC
(Refer to P.21)
Silica type
Shim-pack PREP
(Refer to P.28)
Synthetic high polymer

preparative columns
Polymer type
Shim-pack GPC
(Refer to P.38)
Biological high polymer

preparative columns
Polymer type
Shim-pack PA
(Refer to P.33)
Preparative columns
for general use
Shim-pack
Preparative Columns
Also STR (refer to P.70), Asahipak (refer to P.59) and CAPCELL PAK (refer to P.66) preparative columns are available.
Standard values for maximum sample loads

When the sample load is increased beyond some maximum, extreme peak distortions can occur; the upper limit is the maximum
acceptable sample load based on this peak distortion. The table below shows standard values of maximum sample loads for a semipreparative column and preparative column each having a length of 250mm, in comparison with an ordinary analytical column. It shoud
be noted that these standard values are applicable on condition that various requirements are satisfied: ion suppression for the desired
component is achieved, the desired component is sufficiently soluble in the mobile phase, the type of sample injection solvent is
considered, and other components do not elute near the target peak.
Standard Values of Maximum Sample Loads (column length is 250mm)

Column internal diameter (mm)
Sectional area (cm2)
Flow rate (ml min1)
04.6
0.17
00.8
Maximum sample load (mg)

17
20.0
3.1
15.0
300
50.0
200.0
90.0
2000
Polymer type GPC Preparative Columns

Shim-pack GPC-2000 series
Preparative columns of Shim-pack GPC-800 series (Tetrahydrofuran)
Dimensions
Cat. No.
Shim-pack GPC-2001
Column name
1.5103
20mm i.d.30cm
228-23342-91
Shim-pack GPC-2002
5103
20mm i.d.30cm
228-23342-92
Shim-pack GPC-20025
2104
20mm i.d.30cm
228-23342-93
Shim-pack GPC-2003
7104
20mm i.d.30cm
228-23342-94
(guard column)
8.0mm i.d.5cm
228-20812-94
Shim-pack GPC-2000P
Shim-pack GPC-2000 series

Preparative columns of Shim-pack GPC-800C series (Chloroform)
Dimensions
Cat. No.
Shim-pack GPC-2001C
Column name
1.5103
20mm i.d.30cm
228-23343-91
Shim-pack GPC-2002C
5103
20mm i.d.30cm
228-23343-92
Shim-pack GPC-20025C
2104
20mm i.d.30cm
228-23343-93
Shim-pack GPC-2003C
7104
20mm i.d.30cm
228-23343-94
(guard column)
8.0mm i.d.5cm
228-20812-95
Shim-pack GPC-2000CP
53
Polymer Type Ion Exchange Preparative Column

Shim-pack PA
Column name
Stationary phase
Particle dia. (m)
Dimensions
Cat. No.
Shim-pack PA-DEAE
Diethylaminoethyl group
10
20mm i.d.10cm
228-20758-92
Shim-pack PA-QA
10
20mm i.d.10cm
228-20759-92
Shim-pack PA-CM
Carboxymethyl group
10
20mm i.d.10cm
228-20760-92
Shim-pack PA-SP
Sulfopropyl group
10
20mm i.d.10cm
228-20761-92
Guard Column for Shim-pack PA

Column name
Stationary phase
Particle dia. (m)
Dimensions
Cat. No.
Shim-pack PAG-DEAE
Diethylaminoethyl group
10
8.0mm i.d.1cm
228-20762-91
Shim-pack PAG-QA
10
8.0mm i.d.1cm
228-20763-91
Shim-pack PAG-CM
Carboxymethyl group
10
8.0mm i.d.1cm
228-20764-91
Shim-pack PAG-SP
Sulfopropyl group
10
8.0mm i.d.1cm
228-20765-91
Preparative column for general use

Shim-pack PRC
Column name
Shim-pack PRC-SIL
Shim-pack PRC-SIL(K)
Shim-pack PRC-SIL(L)
Shim-pack PRC-SIL(H)
Shim-pack PRC-ODS
Shim-pack PRC-ODS(K)
Shim-pack PRC-ODS(L)
Shim-pack PRC-ODS(H)
Shim-pack PRC-C8
Shim-pack PRC-C8(H)
Shim-pack PRC-TMS
Shim-pack PRC-TMS(H)
Shim-pack PRC-TMS
Shim-pack PRC-NH2(H)
Shim-pack PRC-CN
Shim-pack PRC-CN(H)
Stationary phase
Particle dia. (m)
Silica
Separation mode
Dimensions
20mm i.d.25cm
30mm i.d.25cm
50mm i.d.25cm
20mm i.d.25cm
20mm i.d.25cm
30mm i.d.25cm
50mm i.d.25cm
20mm i.d.25cm
20mm i.d.25cm
Cat. No.
228-23461-93
228-23461-94
228-23461-95
228-23461-91
228-23464-93
228-23464-94
228-23464-95
228-23464-91
228-24381-93
20mm i.d.25cm
20mm i.d.25cm
Reversed
20mm i.d.25cm
20mm i.d.25cm
Reversed, normal, ion exchange
20mm i.d.25cm
20mm i.d.25cm
20mm i.d.25cm
228-24381-91
228-24382-93
228-24382-91
228-24383-93
228-24383-91
228-24384-93
228-24384-91
15
Adsorption
Octadecyl group
Octyl group
Trimethyl group
Aminopropyl group
Cyanopropyl group
15
Reversed
5
15
5
15
5
15
5
15
5
Reversed
PRC guard column
54
Column name
Stationary phase
Particle dia. (m)
Dimensions
Cat. No.
Shim-pack GPRC-SIL
Shim-pack GPRC-ODS
Shim-pack GPRC-C8
Shim-pack GPRC-TMS
Shim-pack GPRC-NH2
Shim-pack GPRC-CN
Shim-pack GPRC-SIL(K)
Shim-pack GPRC-SIL(L)
Shim-pack GPRC-ODS(K)
Shim-pack GPRC-ODS(L)
Silica
Octadecyl group
Octyl group
5
5
5
8mm i.d.1.5cm
8mm i.d.1.5cm
8mm i.d.1.5cm
228-23462-92
228-23465-92
228-24386-92
Trimethyl group
Aminopropyl group
5
5
8mm i.d.1.5cm
8mm i.d.1.5cm
228-24387-92
228-24388-92
Cyanopropyl group
Silica
Silica
Octadecyl group
Octadecyl group
5
15
15
15
15
8mm i.d.1.5cm
30mm i.d.7.5cm
50mm i.d.5cm
30mm i.d.7.5cm
50mm i.d.5cm
228-24389-92
228-23462-93
228-23462-94
228-23465-93
228-23465-94
Shim-pack
Preparative purification of Paeoniflorin in paeony root
Paeoniflorin
Fig. 82
Fig. 83
Large scale preparative conditions

Sample:
Paeonyroot etract, 200mL, pumped-injection
Column:
Shim-pack PREP-ODS (L) (50mm i.d.25cm)
Mobile phase:
Acetonitriles/water (1/6)
Flow rate:
100mL/min.
UV spectrophotometric detector (230nm, 0.5mmL. cell)
Detector:
Fraction purity test condition

Sample:
Fraction 5L
Column:
Shim-pack CLC-ODS (M) (4.6mm i.d.25cm)
Mobile phase:
Acetonitriles/water (1/6)
Flow rate:
0.8mL/min.
UV spectrophotometric detector (230nm, 10mmL. cell)
Detector:
Preparative purification of Paeonol in Moutan Bark

Paeonol
Fig. 84
Large scale preparative conditions
Sample:
Moutan bark extract, 200mL, pumped-injection
Shim-pack PREP-ODS (L) (50mm i.d.25cm, 15m)
Column:
Mobile phase: 0.1(v/v)Acetate water/Acetonitriles (3/2)
Flow rate:
60mL/min.
Detector:
Fig. 85
Fraction purity test condition
Sample:
Fraction 10L
Column:
STR ODS-H (4mm i.d.15cm, 5m)
Mobile phase:
0.1(v/v)Acetate water/Acetonitriles (3/2)
Flow rate:
0.5mL/min.
Detector:
Preparative purification of Peptides

When separating peptides in reversed phase chromatography,
changing mobile phase into acidic makes it possible to restrain
the sticking of peptides to the surface of the silica gel. In case of
preparative purification, the point is that evaporating a fraction
easily to make the mobile phase acidic using trifluoroacetic acid
and acetate
Preparative Scale Purification of Peptides
Column
Reversed phase TMS(C1), C4, C8, ODS(C18)

(size-exclusion or ion exchange column, as required)
Mobile phase
Acetonitrile/water (or 2-propanol), in the presence of 0.1

to 1(v/v) trifluoreacetic acid, formic acid or acetic acid.
Detector
210-230nm
Fraction
concentration
Evaporator (organic solvent) freeze-drying

(water) storage at 20
Fig. 86 Preparative purification of synthetic Peptides

Sample:
Column:
Mobile phase:
Synthetic peptide (10 amino acids) 100mg/10mL aqueous solution

Shim-pack PREP-ODS(H)kit (20mm i.d.250mm L.)
A: 0.1(v/v) aqueous trifluoroacetic acid
B: 0.1(v/v) sloution of trifluoroacetic acid in acetonitrile
Gradient B 8 18 (20 min)
Flow rate:
15mL/min.
Detector:
220nm
55
Preparative in using GPC column

Various HPLC conditions must be accepted to increase the
sample load. One of these conditions, mobile phase that has high
sample solubilities should be used. In this point, GPC using
chloroform as mobile phase has an advantage rather than
reversed phase chromatography. With reference to the
separation of low molecular, GPC is inferior to reversed phase
chromatography. However, recycling method might be used to
improve separations when the difference of the molecular size
can be noticed and peak top is separated.
Peaks
. Didecylphthalate
. Diheptylphthalate
. Dibutylphthalate
Fig. 87
Separation of Esters phthalate in using GPC column
Column:
Shim-pack GPC 2001C (20mm i.d.30cm)2
Mobile phase:
Chloroform
Flow rate:
3mL/min.
Detector:
Recycling in preparative purification

Since preparative purification columns become more expensive as their size increases, it may be inevitable to use a column of such
length that high resolution is not ensured. In these cases, an enhanced separating effect can be obtained as if the column length were
increased by re-introducing the eluate band containing the desired component eluted from the column back into the column inlet. Figure
88 shows a flow chart for the recycling procedure. Switching the recycle valve permits choice of the out flow path leading to the fraction
collector and the recycling path leading to the pump inlet. In the recycling mode, no additional mobile phase is consumed at all.
pump
sample injector
Preparative
purification column
UV absorption detector
Automatic recycle valve
Recycling path
Out flow path
Fraction collector
Mobile phase
Fig. 88
Flow Chart of Recycling System
One
Eight
Fig. 89
Sample:
56
Recycle separation chromatogram of Styrene oligama
Polystyrene molecular weight marker (molecular weight

760) 1(W/V) chloroform solution 0.5mL injection
Column:
Shim-pack GPC-20025C2002C2001C
Mobile phase:
Chloroform
Detector:
Refractive index detector (128126RIUFS)
Shim-pack
Shim-pack BIO(T)
The
Shim-pack BIO(T) series columns, using titanium column tubing, have been developed for biocompatible HPLC
system, e.g. the Shimadzu LC-7A System.

The
Shim-pack BIO(T) series columns are available in three types: reversed phase, ion exchange, and hydrophobic.
Merits of Titanium Column
Corrosion Resistancy Comparison of Titanium and Stainless Steel
In the HPLC of biological samples, corrosive liquids such as

sodium chloride solution and hydrochloric acid are often used
Solvent
1
10
25
25
Sulfuric acid
1
10
25
25
Nitric acid
25
65
boiling
boiling
Acetic acid
10
60
boiling
boiling
Formic acid
Sodium chloride
10
25
25
25
Ammonium chloride
Zinc chloride
40
20
25
25
Ferrous chloride
Sodium hypochlorite
30
5
25
25
chloride solution and hydrochloric acid, especially against that

by halogen ions, than the 316 stainless steel which is generally
used in HPLC systems.
The Shim-pack BIO(T) series is recommended for such cases.
Pure
titanium
Hydrochloric acid
as the mobile phase.

Titanium is far more resistant against corrosion by sodium
Concentration Temperature 316 stainless

(%)
()
steel
: Less than 0.125mm/year

: 0.5 1.25mm/year
: 0.125 0.5m/year
: More than 1.25mm/year
Shim-pack BIO(T) Series

Column name
Shim-pack CLC-ODS(T)
Shim-pack WAX-1T
Shim-pack WAX-2T
Shim-pack WCX-1T
Shim-pack HPC-C2T
Shim-pack HPC-C3T
Separation mode
Reversed
Anion exchange
Anion exchange
Cation exchange
Hydrophobic
Hydrophobic
Particle dia. (m)

5
3
5
5
5
5
Pore diameter ()
100
100
300
300
300
300
Dimensions
4.6mm i.d.15cm
4.6mm i.d.5cm
4.6mm i.d.5cm
4.6mm i.d.5cm
4.6mm i.d.5cm
4.6mm i.d.5cm
Cat. No.
228-18062-91
228-18257-91
228-18258-91
228-18259-91
228-18260-91
228-18261-91
57
Shim-pack BIO(T)
Peaks
. Ovalbumin
. Myoglobin
. Ribonuclease A
. Lysozyme
Peaks
. Myoglobin
. Carbonic anhydrase
. Transferrin
. -Lactalbumin
. Trypsin inhibitor
Fig. 90
Analysis of Protein Standards
Column:
Shim-pack WAX-2T (4.6mm i.d.5cm)
Mobile phase:
Flow rate:
1.0mL/min.
Detector:
Fig. 91
Analysis of Protein Standards
Column:
Shim-pack WCX-1T (4.6mm i.d.5cm)
Mobile phase:
Phosphate buffer solution (pH 6.0)/sodium chloride,
gradient elution
Flow rate:
1.0mL/min.
Detector:
IgG
Fig. 92 Determination of IgG Monoclonal Antibody in Mouse Ascites Fluid

Column:
Shim-pack WAX-2T (4.6mm i.d.5cm)
Mobile phase:
Flow rate:
1.0mL/min.
Detector:
58
Fig. 93
Analysis of Decomposition Products of Trypsin in Cytoch C
Column:
Shim-pack CLC-ODS(T) (4.6mm i.d.5cm)
Mobile phase:
0.1% aqueous solution of TFA/0.1% acetonitrile
solution of TFA, gradient elution
Flow rate:
1.0mL/min.
Detector:
Asahipak
Asahipak ODP
The
Asahipak ODP column is packed with synthetic hard polymer particles on which octadecyl groups are chemically
bonded and is used for reversed phase LC.

Having
no silanol groups, this column is especially effective for the HPLC of basic compounds, e.g. some drugs.
The
column is stable in a pH range of 213. It can be washed with an alkaline solution.
The
gel has a minimum swelling and contraction, and so the column can be used in a wide mobile phase condition, from
100% water to 100% water-soluble organic solvent.

High
repeatability in gradient LC.
Asahipak ODP
Column name
Asahipak ODP-50
Dimensions
GLC Cat. No.
4D
Stationary phase
4.6mm i.d.15cm
406-020
4E
4.6mm i.d.25cm
406-021
6.0mm i.d.15cm
406-017
6.0mm i.d.25cm
406-018
6D
C18 group
Particle dia. (m)
6E
Guard Column (Analytical)

Column name
Asahipak ODP-50G
Stationary phase
C18 group
Particle dia. (m)

5
Dimensions
GLC Cat. No.
4.6mm i.d.1cm
406-022
6.0mm i.d.1cm
406-019
GLC Cat. No.

Column name
Asahipak C8P-50
Stationary phase
Particle dia. (m)
Dimensions
4.6mm i.d.15cm
406-023
C8 group
4.6mm i.d.25cm
406-024
4.6mm i.d. 1cm
406-025
Asahipak C8P-50G
Asahipak C4P
Column name
Asahipak C4P-50
Stationary phase
C4 group
Particle dia. (m)

5
Dimensions
GLC Cat. No.
4.6mm i.d.15cm
406-026
4.6mm i.d.25cm
406-027

Column name
Stationary phase
Particle dia. (m)
Dimensions
GLC Cat. No.
Asahipak C4P-50G
C4 group
4.6mm i.d.1cm
406-028
59
Peaks
. Ethylene glycol
. Butanol
. Hexanol
. Octanol
. 1-Decanol
. 1-Dodecanol
. 1-Tetradecanol
Fig. 94
Separation of Alkylalcohols Group
Column:
Asahipak ODP-50, C8P-50, C4P-50 (4.6mm i.d.15cm)
Mobile phase:
Methanol 80/water 20
Flow rate:
0.6mL/min.
Detector:
Peaks
. -Endorfin
. Insulin
. Big gastrin
Peaks
. Substance P
. -Endorfin
. Insulin b chain
. Insulin
Fig. 95
Separeation of Macromolecule Peptide
Column:
Asahipak C8P-50, (4.6mm i.d.15cm)
Mobile phase:
0.05TFA (pH2.3) 73/Acetonitriles 27
Flow rate:
0.6mL/min.
Detector:
Fig. 96
Separeation of Macromolecule Peptide
Column:
Asahipak C4P-50, (4.6mm i.d.15cm)
Mobile phase:
0.05TFA (pH2.3) 72/Acetonitriles 28
Flow rate:
0.6mL/min.
Detector:
Peaks
. Vitamin D2
. Vitamin D3
Peaks
. Creatinine
. Methyl benzoate
. n-Propyl benzoate
. n-Hexyl benzoate
Fig. 97
Analysis of Standard Samples
Column:
Asahipack ODP-50 (6.0mm i.d.15cm)
Mobile phase:
Flow rate:
1.0mL/min.
Detector:
60
Fig. 98
Separation of Vitamin D2 and D3
Column:
Asahipak ODP-50 (6.0mm i.d.15cm)
Mobile phase:
Flow rate:
1.0mL/min.
Detector:
Asahipak
Peaks
. Vitamin B6
. Vitamin B1
. Vitamin B2
. Vitamin B12
Fig. 99
Separation of Vitamins B Group
Column:
Asahipak ODP-50, (6.0mm i.d.15cm)
Mobile phase:
Acetonitriles 1/50mM phosphoric acid sodium
Flow rate:
1.0mL/min.
Detector:
Peaks
. Quinine
. Cinchonine
Fig. 100
Separation of Quinine and Cinchonine
Column:
Mobile phase:
Methanol/acetonitrile/10mM sodium phosphate
(2/1/1) (pH 9)
Flow rate:
1.0mL/min.
Detector:
Peaks
. Ribonuclease
. Cytochrome C
. Lysozyme
. Ovalbumin
Fig. 101
Separation of Protein Standard
Column:
Mobile phase:
Solvent A: Water and 0.05 TFA
Solvent B: Acetonitrile and 0.05 TFA
A/BLinear gradient from 90/10 to 40/60, 30minutes
Flow rate:
1.0mL/min.
Detector:
Fig. 102 Analysis of Decomposition Products of Trypsin in Cytochrome C

Column:
Mobile phase:
Solvent A: Water and 0.1 TFA
Solvent B: Acetonitrile and 0.1 TFA
Linear gradient from solvent A to solvent B in 100minutes.
Flow rate:
1.0mL/min.
Detector:
Peaks
. Gly-Gly-Ala
. Gly-Gly-Leu
. Met-Enkephalin
. Leu-Enkephalin
Fig. 103
Analysis of Peptide Mixture
Column:
Mobile phase:
0.05% TFA/acetonitrile (4/1)
Flow rate:
1.0mL/min.
Detector:
Peaks
. Lys-Bradykinin
. Bradykinin
. Met-Enkephalin
. Neurotensin
. Leu-Enkephalin
. Substance P
. Bacitracin
. Insulin
. Insulin B chain
10. Lysozyme
11. Mastoparan
12. Myoglobin
Fig. 104
Analysis of Peptide Mixture
Column:
Mobile phase:
Solvent A: 0.05% TFA/acetonitrile (4/1)
Solvent B: 0.05% TFA/acetonitrile (1/1)
Linear gradient from solvent A to solvent B in 20 minutes
Flow rate:
1.0mL/min.
Detector:
61
Asahipak GS
The
Asahipak GS series columns are packed with hard gel particles made of vinyl alcohol polymer.
The
allowable pH range of mobile phase is 212 for the GS-320 and GS-520, and 29 for the other columns.
Various
organic solvents can be used.
Aqueous
solutions of salts and buffer solutions can be used as mobile phase. (It is recommended that the concentration is
below 0.5M.) Mobile phases that contain urea, guanidine HCI, or SDS (sodium dodecyl sulfate) are also applicable.
High-Resolution Type (7.6mm i.d.30cm)

Type
Applicable both to
aqueous solutions and
organic solvent
Applicable to aqueous
solutions
Column name
Average particle diameter

(m)
GF-310HQ
GF-510HQ
5
5
GF-710HQ
GF-7MHQ
Exclusion limit
(molecular weight)
(Pullulan)
Number of
theoritical plates
GLC Cat. No.
40,000
300,000
16,000
16,000
406-323
406-324
9
7
1,000,000
1,000,000
10,000
11,000
406-325
406-326
GS-220HQ
GS-320HQ
6
6
3,000
40,000
16,000
16,000
406-318
406-319
GS-520HQ
GS-620HQ
7
7
300,000
2,000,000
15,000
15,000
406-320
406-321
Guard Columns
Column name
62
Dimensions
Grade
GLC Cat. No.
GF-1G 7B
7.6mm i.d.10mm
310, 310HQ, 510, 510HQ

710, 710HQ, 700, 710HQ
406-327
GS-2G 7B
7.6mm i.d.10mm
220, 220HQ, 320, 320HQ

520, 520HQ, 620, 620HQ
406-322
Asahipak
Applicable Organic Solvents

Solvent conditions
Solvent
Applicability
Concentra- 310, 510

710, 7M
tion
Aqueous solution 00.5M
Applications
320
520
220
620
GS-710
Methanol
0100%
Below 20%
Ethanol
0100%
Below 20%
050%
51100%
THF
0100%
DMF
0100%
Acetone
0100%
Chloroform
0100%
050%
51100%
Acetonitrile
DMSO
Not applicable
310
Hydrophobic polymer, oligomers, monomers,

surfactants, steroids, hydrophilic polymers
510
Proteins, nucleic acids, sugars, gelatins,

hydrophobic/hydrophilic polymers
220
Oligosaccharides, peptides, hydrophilic,

oligomers, monomers
320
Peptides, nucleic acid constituents, sugars,

vitamines, blood metabolites
520
Plasma proteins, albumins, globulin, sugars,

hydrophilic polymers, nucleic acids
620
Highly polymerated proteins, nucleic acids,

gelatins
710
Polysaccharides, dextran, hydrophilic polymers
Calibration Curves
Fig. 105
Fig. 106
Columns for Aqueous Mobile Phases
Columns for Aqueous/Organic Solvent Mobile Phases
63
Asahipak ES
The
Asahipak ES series columns, developed for high-speed ion exchange chromatography, are packed with vinyl alcohol
polymer particles into which ion exchange groups have been introduced.
Since
the gel particles have many ion exchange groups and alcoholic hydroxyl groups, hence have little hydrophobic
adsorption, the columns are suitable for the separation of ionic biological compounds.
The
columns ensure high yield for proteins, enzymes, and nucleic acids.
The
allowable pH range of mobile phase is 212.
The
columns are available in two types, anion exchange type and cation exchange type.
Analytical Column (7.6mm i.d.10cm)

Column name
Average particle diameter

(m)
Ion exchange group
Ion exchange capacity

(meq/g)
Number of
theoritical plates
GLC Cat. No.
ES-502C
ES-502N
90.5
90.5
Carboxyl group
Diethylamino ethyl group
0.550.02
0.550.02
3,000
3,000
406-109
406-108
The use of preparative guard column GS-20G (228-18754-02) is recommended.
Peaks
. Globulin
. Uric acid
. Mercaptoalbumin
. Nonmercaptoalbumin
Peaks
. Myoglobin
. Ribonuclease A
. Lysozyme
Fig. 107
Column:
Asahipak ES-502C (7.6mm i.d.10cm)
Mobile phase:
Solvent A: Sodium phosphate (pH 7.0)
Solvent B: 50mM sodium phosphate and 500mM
sodium chloride (pH 7.0)
Linear gradient from solvent A to solvent B in 20 minutes
Flow rate:
1.0mL/min.
Detector:
64
Fig. 108
Determination of Mercaptoalbumine and

Nonmercaptoalbumine in Control Serum
Column:
Asahipak ES-502N (7.6mm i.d.10cm)
Mobile phase:
50mM N-methyl piperazine, 400mM sodium sulfate,
and 0.3% ethanol (pH 4.8)
Flow rate:
1.0mL/min.
Detector:
Asahipak
Asahipak NH2P
The
Asahipak NH2P series columns are for sugar analysis, packed with vinylalcoholcopolymer with polyamines chemicalybonded.
High number of theoritical plates and symmetrical peak can be aquired between monosaccharides and origosacharides.
Available under the condition of the alkali (less than pH13) and you can wash the column easily as well as getting high
separation.
The column is stable in a pH range of 213.
Asahipak NH2P
Column name
Stationary phase
Particle dia. (m)
Dimensions
GLC Cat. No.
Asahipak NH2P-50 4E
NH2 group
4.6mm i.d.25cm
406-030

Column name
Stationary phase
Particle dia. (m)
Dimensions
GLC Cat. No.
Asahipak NH2P-50G 4A
NH2 group
4.6mm i.d.1cm
406-031
Peaks
. Glucose
. Maltose
. Maltotriose
. Maltotetraose
. Maltopentaose
. Maltohexaose
. Maltoheptaose
Peaks
. Fructose
. Glucose
. Sucrose
. Lactose
Fig. 109
Separation of Monosaccharides and Disaccharides
Column:
Asahipak NH2P-50, (4.6mm i.d.25cm)
Mobile phase:
Acetonitriles 75/Water 25
Flow rate:
1.0mL/min.
Detector:
Fig. 110
Peaks
. Glucose
. Isomaltose
. Isomaltotriose
. Isomaltotetraose
. Isomaltopentaose
. Isomaltohexaose
. Isomaltoheptaose
. Isomaltooctaose
. Isomaltononaose
Peaks
. Ramnose
. Fructose
. Glucose
. Sucrose
. Maltose
. Rafinose
Fig. 111 Separation of Monosaccharides, Disaccharides and Trisaccharides

Column:
Mobile phase:
Flow rate:
1.0mL/min.
Detector:
Separation of Oligosaccharides
Column:
Mobile phase:
Flow rate:
1.0mL/min.
Detector:
Fig. 112
Separation of Dextran hydrolysis substances
Column:
Mobile phase:
Acetonitriles/Water
Flow rate:
1.0mL/min.
Detector:
65
CAPCELL PAK
(1) Features of CAPCELL PAK
As covered with silicones polymer on the surface of silica, superior in chemical stability rather than silica type materials.
(2) CAPCELL PAK is classified as follows.
AG Type
Only 120 Type pore diameter
SG Type
120 and 300 Type pore diamete
CAPCELL PAK
(3) Selection of CAPCELL PAK columns

SG type
AG type
Aliphatic carboxylic acids and esters
Aromatic carboxylic acids and esters
Other compounds
Flavonol Oil-soluble viamine
Catechin group Porphyrin
Dansyl amino acid
Steroid
Aromatic hydrocarbons
Catecholamine
Nucleic acids base
Nucleoside
Peptide
Saponin
Amines
Quaternary ammonium salt
Proteins
Water-solved vitamine
As for the amount of micro-metal involved in silica, SG type has fewer than AG type.
CAPCELL PAK AGType (C18 Type)

Stationary phase
Pore diameter ()
Particle dia. (m)
Column i.d.
4.6
C18
120
6.0
20
30
66
Column length (mm) GLC Cat. No.

35
12501
150
12503
250
12504
35
12506
150
50
12508
15501
250
250
15504
16501
CAPCELL PAK
CAPCELL PAK AGType (C8 Type)

Stationary phase
Pore diameter ()
Particle dia. (m)
Column i.d.
4.6
C8
120
6.0
20

35
21501
150
250
21503
21504
35
150
50
250
21506
21508
25501
25503
CAPCELL PAK SGType (C18 Type)

Stationary phase
Pore diameter ()
Particle dia. (m)
Column i.d.
4.6
C18
120
6.0
20
30
4.6
C18
300
6.0
20

35
12510
150
12512
250
12513
35
13510
150
50
13512
16510
250
250
35
150
250
35
150
50
250
16513
16501
12520
12522
12523
13520
13522
16520
16523
67
Peaks
. Methyiparabon
. p-Hydroxy benzoic acid
. Ethylparaben
. Dehydroacetic acid
. n-Propylparaben
. Sorbic acid
. Benzoic acid
. iso-Butylparaben
. n-Butylparaben
10. Salicylic acid
Peaks
. Ascorbic acid
. Calcium pantothenate
. Nicotinamide
. Pyridoxine hydrochloride
. Caffeine
. Folic acid
. Biotin
. Riboflavin
. Thiamine
Fig. 113
Separation of Aqueous Vitamine Group
Fig. 114
Column:
C18 SG120 (4.6mm i.d.25cm)
Mobile phase:
Water (pH2.1, H3PO4)/CH3CN
9/1, 1.5mM heptanesulfonic acid
Flow rate:
1.5mL/min.
Detector:
Separation of an Antiseptic Component
Column:
C18 SG120 (4.6mm i.d.25cm)
Mobile phase:
0.05M NaH2PO4(pH4.5)/MeOH/CH3CN50/35/15,
4mM cetyltrimethylammonium chloride
Flow rate:
1mL/min.
Detector:
Injection volume:
10L (5g/mL)
CAPCELL PAK SG Type (C8 Type)

Stationary phase
Pore diameter ()
Particle dia. (m)
Column i.d. (mm)

4.6
C8
120
6.0
20
4.6
C8
300
6.0
20

35
21511
150
21513
250
21514
35
21516
150
21518
50
25511
250
25513
35
21521
150
21523
250
21524
35
21526
150
21528
50
25521
250
25523
Peaks
. Ribonuclease A
. Cytochrome C
. Lysozyme
. BSA
. Myoglobin
. Ovalbumin
Column
Column
Fig. 115 Separation of Protein with SG type columns

Mobile phase:
(A) 0.1 TFA/H2O (B) 0.1 TFA/CH3CN
(B) 15 60 (15min) Gradient
Flow rate:
1.5mL/min.
Detector:
68
Zorbax
Zorbax
The
The
Zorbax columns are high-performance columns developed by DuPont, the pioneer in HPLC.
columns are packed with fully-porous, spherical silica particles on which the respective stationary phases are
chemically bonded. (Except Zorbax SIL)
Zorbax
Column name
Stationary phase
Particle dia. (m)
Separation mode
Silica
Adsorption
Octadecyi group
Reversed
Octyl group
Reversed
Trimethyl group
Reversed
Zorbax SIL
Zorbax ODS
Zorbax C8
Zorbax TMS
Zorbax NH2
Aminopropyl group
Reversed, normal,
anion, exchange
Zorbax CN
Cyanopropyl group
Reversed, normal
Zorbax SAX
Quaternary
ammonium group
Anion exchange
Sulfonic group
Cation exchange
Zorbax SCX-300
Dimensions
GLC Cat. No.
4.6mm i.d.15cm 883952-701
4.6mm i.d.25cm
4.6mm i.d.15cm
880952-701
883952-702
4.6mm i.d.25cm
4.6mm i.d.15cm
880952-702
883952-706
4.6mm i.d.25cm
4.6mm i.d.15cm
880952-706
883952-710
4.6mm i.d.25cm
880952-710
4.6mm i.d.15cm
4.6mm i.d.25cm
883952-708
880952-708
4.6mm i.d.15cm
883952-705
4.6mm i.d.25cm
4.6mm i.d.15cm
4.6mm i.d.25cm
880952-705
883952-703
880952-703
4.6mm i.d.15cm
4.6mm i.d.25cm
883952-704
880952-704
Guard Column Pre-column Kit

In analyses with a Zorbax column, it is recommended to use a
guard column or a pre-column so that a longer service life is
expected of the Zorbax column.
The kit consists of an empty column, packing material, and a
filter.
Fig. 116
Flow Line Having Guard Column and Pre-column
69
STR ODS Series

STR
(STR : Shimadzu Techno Research)
ODS Series columns using octadedecyl group as the stationary phase feature low cost and wide field of applications.
The STR ODS Series is available in the following three types:

STR ODS-H
STR ODS -M
STR ODS
STR ODS -
19% in carbon contents.

Suitable for analysis of less-polar/basic compounds.
15% in carbon contents.

Suitable for analysis of highly polar/acidic compounds.
17% in carbon content, and uses high-purity (99.999%) silica gel

Highly resistant against acids and alkalis.
The STR ODS- Columns

STR ODS Column for Analytical HPLC
Column name
Pore dia. ()
Particle dia. (m)
Column i.d. (mm)

4.0
STR ODS-
120
4.6
6.0
STR ODS- PEEK
120
Column length (mm)

150
GLC Cat. No.

404-019
250
150
250
404-018
404-021
404-020
150
150
250
404-022
404-027
404-026
4.6
Particle dia. (m)

5
Column i.d. (mm)

20.0
Column length (mm)

250
GLC Cat. No.

404-030
Pore dia. ()
Particle dia. (m)
STR ODS-
120
STR ODS- PEEK
120
Column i.d. (mm)

4.0
4.6
6.0
4.6
Column length (mm)

10
10
10
10
GLC Cat. No.

404-023
404-024
404-025
404-028
Particle dia. (m)

5
Column i.d. (mm)

20.0
Column length (mm)

50
GLC Cat. No.

404-031
STR ODS Column for Preparative HPLC

Column name
STR ODS-
Pore dia. ()
120
Guard Column for Analytical HPLC

Column name
Guard Column for Preparative HPLC

Column name
STR ODS-
70
Pore dia. ()
120
STR
STR ODS Columns
1. Analytical HPLC
Column name
Pore dia. ()
Particle dia. (m)
ODS-H
100
Column i.d. (mm)

4
4.6
4
ODS-M
100
5
4.6
Column length (mm)

50
150
250
150
250
150
250
150
250
Cat. No.
228-21336-02
228-21336-01
228-21336-03
228-21336-04
228-21336-05
228-21830-01
228-21830-02
228-21830-03
228-21830-04
Column length (mm)

250
50
250
250
50
250
Cat. No.
228-21831-01
228-21831-02
228-21831-03
228-21832-01
228-21832-02
228-21832-03
2. Preparative HPLC
Column name
Pore dia. ()
Particle dia. (m)
PREP ODS-H
120
Column i.d. (mm)

20
4.6
PREP ODS-M
120
20
4.6
STR ODS-H
Peaks
. Vitamin A
. Vitamin D2
. Vitamin E
Peaks
. Formaldehyde
. Acetaldehyde
. Propylaldehyde
. Butylaldehyde
. Pentanal
Fig. 117
Analysis of Aldehydes
Fig. 118
Column:
ODS-H (4.0 mm i.d.15 cm)
Mobile phase:
Flow rate:
0.8 mL/min.
Detector:
Fluorophotometric detector (Ex: 360 nm, Em: 440nm)
Analysis of Oil-Soluble Vitamines
Column:
Mobile phase:
Methanol
Flow rate:
0.5 mL/min.
Detector:
UV spectrophotometric detector (260 nm)
Peaks
. Asp
. Glu
. Asn
. Gln
. Ser
. Thr
. His
. Gly
. Ala
10. Tyr
Fig. 119
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
Tyr
Met
Val
Pro
Arg
Trp
Phe
Lys
Ile
Leu
Analysis of PHT-Amino Acids
Column:
Mobile phase:
10mM Sodium formate buffer solution (pH 5.50 and
containing 0.070% SDS)/acetonitrile (60/40)
Flow rate:
0.5 mL/min.
Detector:
71
STR ODS-
Peaks
. Ethosuximide
. Primidone
. Phenobarbital
. Phenytoin
. Carbamazepin
Peaks
. Nicotinic acid
. Nicotinamide
. Calcium pantothenate
. Pyridoxine
. Ribofiavin phosphate
. Thiamine
. Caffeine
. Ribofiavin
Fig. 120
Analysis of Water Soluble Vitamines
Column:
ODS- (4.0 mm i.d.15 cm)
Mobile phase:
100mM phosphate buffer solution (pH 2.1) and
1.5mM Sodium octane sulfonate (9/1)
Flow rate:
1.0 mL/min.
Detector:
Fig. 121
Analysis of Anticonvulsant
Column:
ODS- (4.0 mm i.d.15 cm)
Mobile phase:
/methanol/acetonitrile (4/2/1)
Flow rate:
1.0 mL/min.
Detector:
STR ODS-M
Peaks
. Benzoic acid
. Sorbic acid
Peaks
. Tartaric acid
. Malic acid
. Malonic acid
. Acetic acid
. Citric acid
. Succinic acid
. Propionic acid
Fig. 122
Fig. 123
Analysis of Organic Acids
Column:
ODS-M (4.6 mm i.d.15 cm)
Mobile phase:
10mM KH2 PO4 (adjusted to pH 2.3 with
phosphoric acid)
Flow rate:
1.0 mL/min.
Detector:
Analysis of Food Presevatives
Column:
ODS-M (4.6 mm i.d.15 cm)
Mobile phase:
25mM Phosphate buffer solution (pH 3.5)/methanol
(7/3)
Flow rate:
0.8 mL/min.
Detector:
STR PREP ODS-H

Peaks
. Uracil
. Methyl benzoate
. Naphthalene
. Biphenyl
Fig. 124
Inspection Data
Column:
PREP ODS-H (20 mm i.d.25 cm)
Mobile phase:
Flow rate:
8.0 mL/min.
Detector:
20L
72
Fig. 125 Preparative LC Chromatogram of Paeoniflorin in Peony Extract

Column:
PREP ODS-H (20 mm i.d.25 cm)
Mobile phase:
Water/acetonitile (6/1)
Flow rate:
15 mL/min.
Detector:
Sample size:
100 mg of peony extract (2.0 mL of solution)
4 Application Data
Food Industry
Peaks
. Acetate
. Lactate
. Succinate
. Cl
. Malate
. NO3
. Citrate
. Tartarate
. SO42
Fig. 126
Analysis of Anions in Wine
Peaks
. Fructose
. Glucose
. Sucrose
Fig. 127
Column:
Shim-pack IC-A1 (4.6 mm i.d.15 cm)
Mobile phase:
1.2mM potassium biphthalate (pH 4.2)
Flow rate:
1.5 mL/min.
Detector:
Peaks
. Maltose
. Fructose
. Galactose
. iso-Maltose
. Glucose
Peaks
. Sucrose
. Glucose
. Fructose
. Sorbitol
Fig. 128
Determination of Saccharides in Pickles
Column:
Shim-pack SCR-101C (7.9 mm i.d.30 cm)
Mobile phase:
Water
Flow rate:
1.0 mL/min.
Detector:
Fig. 129
Determination of Saccharides in Miso (Bean paste)
Column:
Shim-pack ISA-07 (4.0 mm i.d. 25 cm)
Mobile phase:
Potassium borate buffer solution, gradient elution
Flow rate:
0.6 mL/min.
Detector:
Fluorescence detector (Ex. 320nm, Em.430nm)
Method:
Post-column derivatization with arginine
Peaks
. -Tocopherol
. -Tocopherol
. -Tocopherol
. -Tocopherol
Peaks
. Malic acid
. Succinic acid
. Lactic acid
. Formic acid
. Acetic acid
. Pyroglutamic acid
Fig. 130
Determination of Saccharides in Soft Drink
Column:
Shim-pack CLC-NH2 (6.0 mm i.d.15 cm)
Mobile phase:
Actonitrile/water(7/3)
Flow rate:
1.2 mL/min.
Detector:
Determination of Organic Acids in Soy Sauce
Column:
Shim-pack SCR-102H (8.0 mm i.d.30 cm)
Mobile phase:
5mM p-toluenesulfonic acid
Flow rate:
0.8 mL/min.
Detector:
Method:
Post-column derivatization with bis-tris buffer
Fig. 131
Determination of Tocopherols in Soy Bean Oil
Column:
Shim-pack CLC-NH2 (6.0mm i.d.15 cm)
Mobile phase:
n-Hexane/isopropanol (25/1)
Flow rate:
1.5 mL/min.
Detector:
73
Peaks
. Hypoxanthine
. IMP
. Inosine
. AMP
. ADP
. ATP
Fig. 132
Analysis of Adenine Derivative 6 Components
Column:
STR ODS- (4.6 mm i.d.15 cm)
Mobile phase:
100mM phosphate (triethylammonium) buffer
solution (pH 6.8) 100/acetonitrile
Flow rate:
1.0 mL/min.
Detector:
Hesperodine
Fig. 133
Determination of Hesperidine in Orange Juice
Column:
Shim-pack CLC-ODS (6.0 mm i.d.15 cm)
0.1M phosphate buffer solution (pH 2.1)/acetonitrile (3/1)
Mobile phase:
Flow rate:
1.5 mL/min.
Detector:
Peaks
. Stevioside
. Rebaudioside A
Fig. 134
Determination of Steviosides in Pickles
Column:
Shim-pack CLC-NH2 (6.0 mm i.d.15 cm)
Mobile phase:
Flow rate:
1.5 mL/min.
Detector:
Peaks
. Benzoic acid
. Sorbic acid
Fig. 135
Peaks
. Asp
. Thr
. Ser
. Glu
. Pro
. Gly
. Ala
. Val
Theanine
Fig. 136
Determination of Theanine in Tea Leaf
Column:
Precolumn:
Shim-pack GRD-ODS
10mM phosphate buffer solution (pH 7.1)/acetonitrile (92/8)
Mobile phase:
Flow rate:
1.5 mL/min.
Detector:
Fluorescence detector (Ex. 365nm, Em. 435nm)
Method:
Prelabelling with OPA
74
Analysis of Preservatives in Food
Column:
Precolumn:
Shim-pack GRD-ODS
Mobile phase:
/acetonitrile (20/1)
Flow rate:
1.5 mL/min.
Detector:
Fig. 137
.
10.
11.
12.
13.
14.
15.
16.
Met
Ile
Leu
Tyr
Phc
His
Lys
Arg
Analysis of Amino Acids in Soy Sauce
Column:
Shim-pack ISC-07 (4.0 mm i.d.15 cm)
Mobile phase:
Sodium citrate buffer solution, gradient elution
Flow rate:
0.3 mL/min.
Detector:
Method:
Prelabelling with OPA
Medicines and Cosmetics
Peaks
. Nicotinic acid
. Nicotinamide
. Pantothenic acid
. Ethofyline (I.S.)
. Pyridoxine
. Caffeine
. Thiamine
. Riboflavine
Fig. 138
Analysis of Vitamin Tablet
Column:
Mobile phase:
0.1M phosphate buffer solution (pH 2.1) and 1.2mM
sodium octane sulfonate/acetonitrile (9/1)
Flow rate:
1.5 mL/min.
Detector:
Ethinylestradiol
Fig. 139
Determination of Ethinylestradiol in Cream
Column:
Shim-pack CLC-SIL(M) (4.6mm i.d.25cm)
Mobile phase:
Flow rate:
1.0 mL/min.
Detector:
Benzothorium
Chloride
Taurine
Fig. 140
Determination of Taurine in Soft Drink
Column:
Shim-pack SCR-101H (7.9mm i.d.30cm)
Mobile phase:
10mM aqueous solution of perchloric acid
Flow rate:
0.8 mL/min.
Detector:
Fig. 141
Determination of Benzethonium Chloride in Gargle
Column:
Mobile phase:
Flow rate:
1.5 mL/min.
Detector:
Berberine
Amygdalin
Fig. 142
Determination of Berberine in Coptis Japonica Powder
Column:
Mobile phase:
10mM phosphate buffer solution (pH 2.6) and 0.1M
sodium perchlorate/acetonitrile (2/1)
Flow rate:
1.5 mL/min.
Detector:
Fig. 143
Determination of Amygdalin in Apricot Seed
Column:
Mobile phase:
Water/acetonitrile (4/1)
Flow rate:
1.5 mL/min.
Detector:
75
lodoform
Peaks
. Camphor
. Methyl salicylate
. Thymol
. Menthol
Fig. 144
Analysis of Antiphlogistic/Analgesic Paste
Column:
Mobile phase:
Methanol/tetrahydrofuran/water (5/1/4)
Flow rate:
1.2 mL/min.
Detector:
Fig. 145
Determination of Iodoform in Pulveres
Column:
Mobile phase:
/acetonitrile (1/2)
Flow rate:
1.5 mL/min.
Detector:
Chlorhexidine gluconate
Chondroitin sulfate
Fig. 146
Determination of Chlorhexidine Gluconate
Column:
Mobile phase:
10mM phosphate buffer solution (pH 2.6) and 0.1M
sodium perchlorate/acetonitrile (3/2)
Flow rate:
1.5 mL/min.
Detector:
Fig. 147
Peaks
. Asp
. Thr
. Ser
. Glu
. Pro
. Cysteine
. Gly
. Ala
. Val
Fluocinolone
acetonide
Fig. 148
Determination of Fluocinolone Acetonide in Cream
Column:
Zorbax CN (4.6mm i.d.25cm)
Mobile phase:
n-Hexane/ethanol(4/1)
Flow rate:
1.0mL/min.
Detector:
UV spectrophotometric detector
76
Determination of Chondroitin sulfate in Eyewash
Column:
Mobile phase:
50mM phosphate buffer solution (pH 3)
Flow rate:
1.0 mL/min.
Detector:
Fig. 149
10.
11.
12.
13.
14.
15.
16.
17.
18.
Met
Ile
Leu
Try
Phe
His
Trp
Lys
Arg
Analysis of Amino Acid in Transfusion Liquid
Column:
Shim-pack ISC-07 (4.0mm i.d.15cm)
Mobile phase:
Citric acid buffer solution, gradient elution
Flow rate:
0.3 mL/min.
Detector:
Method:
Post-column derivatization with OPA
-Glycyrrhrtinic acid
Vitamin B12
Fig. 150
Determination of -Glycyrrhetinic Acid in Cream
Column:
CAPCELL PAK C18 (5m) (4.6mm i.d. 25cm)
Mobile phase:
Acetonitrile/phosphoric acid (pH 2.5) (3/1)
Flow rate:
1.0 mL/min.
Detector:
Fig. 151
Determination of Vitamin B12 in Injection
Column:
Mobile phase:
/acetonitrile (7/1)
Flow rate:
1.5 mL/min.
Detector:
Peaks
. Cl
. NO3
. SO42
Fig. 152
Analysis of Inorganic Anions in Injection
Column:
Shim-pack IC-A1 (4.6mm i.d. 15cm)
Mobile phase:
2.5mM phthalic acid and 2.4mM (tris)
(hydroxymethyl) aminomethane (pH 4.0)
Flow rate:
1.5 mL/min.
Detector:
Peaks
. Acetaminophen
. Caffeine
. Dihydrocodeine phosphate
. Methylephedrine
Fig. 153
Analysis of Cold Medicine
Column:
Mobile phase:
10mM phosphate buffer solution (pH 2.6) and
20mM sodium perchloric/acetonitrile (8/1)
Flow rate:
1.5 mL/min.
Detector:
77
Medical and Biochemical Field
Peaks
. Acetylsalicylic acid
. Salicylic acid
Peaks
. Ethosuximide
. Primidone
. Phenobarbital
. Phenytoin
. Hexobarbital (I.S.)
. Carbamazepin
Fig. 154
Analysis of Anticonvulsants in Serum
Fig. 155 Determination of Acetylsalicylic Acid and Salicylic Acid in Serum
Column:
Mobile phase:
0.1M phosphate buffer solution (pH 5.5)
/methanol(10/9)
Flow rate:
1.2 mL/min.
Detector:
Column:
0.1M phosphate buffer solution (pH 2.1)/methanol (1/1)
Mobile phase:
Flow rate:
1.5 mL/min.
Detector:
Peaks
. Theophyline
. Ethofyline (I.S.)
Fig. 156
Fig. 157 Determination of Xanthine, Hypoxanthine, and Uric Acid in Urine
Determination of Theophylline in Blood
Column:
Mobile phase:
Flow rate:
1.0 mL/min.
Detector:
Peaks
. UMP
. CMP
. AMP
. GMP
. UDP
. CDP
Peaks
. Uric acid
. Hypoxanthine
. Xanthine
.
.
.
10.
11.
12.
Column:
Mobile phase:
20mM phosphate buffer solution (pH 3)
Flow rate:
1.0 mL/min.
Detector:
ADP
UTP
GDP
CTP
ATP
GTP
25-OH-D3
Fig. 158
Analysis of Nucleotides in Rat Liver Oil
Column:
Mobile phase:
Phosphate buffer solution (pH 7), gradient elution
Flow rate:
1.0 mL/min.
Detector:
78
Fig. 159
Determination of Vitamin 25-OH-D3 in Blood
Column:
Mobile phase:
Flow rate:
1.5 mL/min.
Detector:
Peaks
. Vanillylmanderic acid (VMA)
. Homovanillic acid (HVA)
Peaks
. N-Acetylputrescine
. Putrescine
. N1-Acetylspermidine
. N1-Acetylspermine
Fig. 160
Determination of Polyamines in Urine
Fig. 161
Column:
Mobile phase:
Sodium perchlorate and sodium hexane sulfonate
/acetonitril, gradient elution
Flow rate:
1.1 mL/min.
Detector:
Method:
Determination of HVA and VMA in Neonate Urine
Column:
Shim-pack CLC-VMA (6.0mm i.d.15cm)
Mobile phase:
Tartaric acid/acetonitrile (97/3) with a small amount
of EDTA
Flow rate:
1.5 mL/min.
Detector:
Histamine
Fig. 162
Determination of -Glucosidase
Fig. 163
Column:
Mobile phase:
gradient elution
Flow rate:
0.5 mL/min.
Detector:
Determination of Histamine in Plasma
Column:
Mobile phase:
Flow rate:
1.0 mL/min.
Detector:
Method:
Peaks
. Tau
. P-EtNH2
. Thr
. Ser
. Asn
. Glu
. Gln
. Pro
. Gly
10. Ala
11. Cit
12. ABA
13. Val
Fig. 164
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
Met
Cys
Ile
Lou
Tyr
Phe
-Ala
Trp
His
Orn
Lys
Arg
Analysis of Amino Acids in Serum
Column:
Shim-pack ISC-07 (4.0mm i.d.15cm)
Mobile phase:
Lithium citrate buffer solution, gradient elution
Flow rate:
0.4 mL/min.
Detector:
Method:
79
Chemical Industry
Fig. 165
Analysis of Epoxy Resin
Column:
Mobile phase:
Water/acetonitrile, gradient elution
Flow rate:
1.0 mL/min.
Detector:
Fig. 167
Analysis of Alkylamine-Polyethylene Aducts
Column:
Mobile phase:
Methanol/0.05% phosphoric acid (3/2)
Flow rate:
1.0 mL/min.
Detector:
Fig. 166
Analysis of Ester Oligomers
Column:
Mobile phase:
Flow rate:
1.0 mL/min.
Detector:
Fig. 168
Analysis of Polyoxyethylene Octylphenyl Ether
Column:
Shim-pack CLC-SIL (6.0mm i.d.15cm)
Mobile phase:
Flow rate:
1.5 mL/min.
Detector:
Low molecular weight
High molecular weight
Fig. 169
Analysis of Polyvinylacetate
Column:
Shim-pack GPC-804, 803, and 802, (8.0mm i.d.
30cm, each)
Mobile phase:
Tetrahydrofuran
Flow rate:
1.0 mL/min.
Detector:
80
Fig. 170
Analysis of Carboxylmethylcellulose
Column:
Asahipak GS-710 (two columns)
Mobile phase:
50mM sodium nitrate
Flow rate:
1.0 mL/min.
Detector:
lrganox1076
Fig. 171
Analysis of Isoprene Rubber
Column:
Shim-pack GPC-804, 802, and 802 (8.0mm i.d.
30cm, each)
Mobile phase:
Tetrahydrofuran
Flow rate:
1.0 mL/min.
Detector:
Fig. 172
Determination of Irganox 1076 in Rubber
Column:
Zorbax ODS (4.6mm i.d.15cm)
Mobile phase:
Methanol/tetrahydrofuran (9/1)
Flow rate:
1.0 mL/min.
Detector:
Cl
Peaks
. 4-Carboxybenzaldehyde
. p-Toluic acid
Fig. 173
Determination of Impurities in Terephthalic acid
Column:
Mobile phase:
/acetonitrile (4/1)
Flow rate:
1.5 mL/min.
Detector:
Fig. 174
Determination of Chlorine in Concrete Additive
Column:
Mobile phase:
2.0mM phthalic acid and 1.5mM tris
(hydroxymethyl) aminomethane
Flow rate:
1.5 mL/min.
Fe-EDTA
Borate
Fig. 175 Determination of Borate in Plating Solution

Column:
Shim-pack SCR-101H (7.9mm i.d.30cm)
Mobile phase:
5mM perchloric acid aqueous solution
Flow rate:
0.8 mL/min.
Detector:
Reractive index detector
Fig. 176
Determination of EDTA
Column:
STR ODS- (4.6mm i.d.15cm)
Mobile phase:
15mM inclding tributylamines 10mM phosphate
aqueous solution (pH 7) 5/acetonitrile 1
Flow rate:
1.0 mL/min.
Detector:
UV spectrophtometric detector (258nm)
81
Environmental Pollutants
Peaks
. Cl
. NO2
. NO3
. SO42
Fig. 177
Determination of Inorganic Anion in Rain Water
Column:
Shim-pack IC-A3
Mobile phase:
8.0mM Hydroxyacetic benzoate
Flow rate:
1.5 mL/min.
Detector:
Peaks
. Na
. NH4
. K
Fig. 178
Determination of Monovalent Cations in Soil Water
Column:
Shim-pack IC-C1 (5mm i.d.15cm)
Mobile phase:
5mM nitric acid aqueous solution
Flow rate:
1.5 mL/min.
Detector:
Peaks
. Br
. NO3
Phenol
Fig. 179
Determination of Bromine and Nitric Acid
Column:
Mobile phase:
Flow rate:
1.5 mL/min.
Detector:
82
Fig. 180
Determination of Phenol in Water
Column:
Mobile phase:
Flow rate:
1.5 mL/min.
Detector:
Sample pretreatment: Concentrated with an automatic sample
pretreatment system
5 Parts for Flow line

Plumbing parts (Connecting Parts)
Cut and sharpen not necessary
Forged pipe
Fingertightening connector
You can connect the column by tighening this connector, not
by wrench. This PEEK connector which is superior in
chemical compatibility and mechanical strength is suitable for
biological LC. Available to 1.6mm i.d. pipe such as stainless,
titanium, teflon, tefzel, PEEK etc. Maxium pressure is
250kgf/cm2 when connecting to 0.5mm i.d. PEEK pipe.
This 1.6mm o.d. pipe (SS 316) is cut at the size of easily use
and mechanical-forged smoothly in both edges.
Pre-cut Stainless Pipe
Description
Male Nut, PEEK (Inc. 5pcs.)
Connecting
i.d.o.d.length (mm)
0.31.6100
0.31.6200
0.31.6300
0.31.6400
0.31.6500
0.81.6100
0.81.6200
0.81.6300
0.81.6400
0.81.6500
Cat. No.
228-18565-84
by stainless plumbing parts

Ferrule1.6F
SS pipe
Male Nut
Column
Cat. No
228-22301-00
228-22302-00
228-22303-00
228-22304-00
228-22305-00
228-22801-00
228-22802-00
228-22803-00
228-22804-00
228-22805-00
To connect this type of column, use the kit below.
Description
Cat. No.
Number
Pipe 0.3 i.d.1.6 o.d.25cm 1pc.
Column Connection Kit C ASSY
228-16058-91
(For new-new type connection)
Male Nut
1.6MN 2pcs.
Ferrule
1.6F
2pcs.
Connector Plumbing Parts
Male Nut
1.6MN
Female Nut
1.6FN
Coupling1.6C
Union1.6U
Union1.6U(X)
Ferrule1.6F
Description
Cat. No.
Adapter1.6A
Number
Male Nut 1.6MN
228-16001-84
10
Female Nut1.6FN
228-16002-84
10
Coupling1.6C
228-16004-03
Adapter1.6A
228-16005-03
Union1.6U
228-00034
Union1.6U(X)
228-01356
Ferrule1.6F
228-16000-84
Ferrule1.6F
228-16000-85
10
83
LC plumbing part kit

This kit put in a prastic case consists of pipes, connectors and
tools needed to HPLC plumbing parts. It is possible to cut the
pipe with the cutter attached as the length as you like. After
cutting the pipe, hold this in the miniture vice and file the end
of this smooth.
Description
LC plumbing part kit
Cat. No.
228-24209-91
LC Connecting Part Kit

Mark
Description
Male Nut, 1.6MN
Ferrule, 1.6F
Coupling, 1.6C-0.4
Plug, 1.6P
Longbush, 7010-011
Ferrule, 7010-010
PTFE ferrule, 1.6F-T
PTFE tube, o.d. 1.6
SS pipe, o.d.1.6
Pre-cut pipe, o.d. 1.6
Pipe cutter
File (triangle, flat)
Miniture vice
Number
Notes
10
20
2
2
2
For Rheodyne
injector
4
5
2m
i.d. 0.4
Each 2m i.d. 0.3 and 0.8
4
i.d. 0.3, 250 length
1
Each 1
1
Kit
Description
Cat. No.
Coupling 1.6C ASSY
228-16051-91
Adapter 1.6A ASSY
228-16052-91
Union 1.6U Newtype ASSY Type
228-16053-91
Union 1.6U(X) Newtype ASSY
228-16054-91
Column connector kit
228-17943-92
Coupling
Ferrule
Male Nut
Adapter
Ferrule
Male Nut
Female Nut
Union
Ferrule
Female Nut
Union
Frrule
Female Nut
Male Nut
Ferrule
Products Name
1.6C
1.6F
1.6MN
1.6A
1.6F
1.6MN
1.6FN
1.6U
1.6F
1.6FN
1.6U(X)
1.6F
1.6FN
1.6MN
1.6F
Number
1
2
2
1
2
1
1
1
2
2
1
2
2
2
2
Checking the end of pipe

As if cutting a wire by wrench
Cut the stainless pipe easily
Holding and cutting the pipe like wrench by cutter in the LC
plumbing part kit, we can prevent plugging the pipe. You can
cut easily and the end of the pipe is smooth, comparing with
the case of filing. (Do not forget to file the end of the pipe.)
Possible to cut from 0.3mm i.d. to 1.6mm o.d.
Use the pipe once filing the end of the stainless pipe smooth
and checking with a magnifying glass carefully. We can look
carefully with a High magnifying power glass made of noncolour lense.
Description
A magnifying glass, 20 times
Subscription
Piping cutter
84
Cat. No.
670-18802
Cat. No.
670-12327
PEEK plumbing parts

Use this parts for maintenance Non-metal LC system and system up.
Description
Plumbing parts (Connecting parts) kit for inert LC
Cat. No.
228-33285-91
Component of Plumbing parts (Connecting parts) kit for Non-metal LC

Male Nut 1.6MN-2 PEEK
228-332135pcs.
Male NutFerrule separate type
Stop joint PEEK

(228-280902pcs. PEEK Plug)
Ferrule 1.6F PEEK

228-3163810pcs.
Male NutFerrule separate type
Clean cut
228-339301pcs.
A pressing cutter for PEEK tube
Male connector 1.6C PEEK

228-250142pcs.
Coupling made with PEEK
PEEK tube set

(228-334062pcs.)
-1 PEEK tube 1.60.25500 straight
-2 PEEK tube 1.60.25500 L type
-3 FEP tube NFL016

(For protect PEEK tube, 1m)
There are two types: male nutferrule separation type and joint type.(Refer to P.83)
When using PEEK tube with organic solvent type mobile phase, use separationtype.
When using PEEK tube with aqueous solution like IC, use joint type under normal pressure.
The
solvent when using PEEK resin

Although PEEK resin has high mechanical strength and thus is considered as optimal plastic material for HPLC, there are some
restrictions to use. Make sure that particularly the solvents below are NOT used.
Concentrated sulfuric acid, concentrated nitric acid, dichloroacetic acid, tetrahydrofuran(THF), dichloromethane,
chloroform, dimethyl sulfoxide (DMSO), acetone
There is no problem of using methanol and acetonitriles, but use these solvents within the pressure below.
Methanol: Each diameter200kgf/cm2 (19.6MPa)
Acetonitriles: i.d. 0.125/0.25200kgf/cm2 (19.6MPa)
i.d. 0.50
150kgf/cm2 (14.7MPa)
i.d. 0.75
100kgf/cm2 (9.8MPa)
Each PEEK tube showed in this page includes FEP tube for protection. Preparing for the case of PEEK tube is broken, be
sure to put FEP tube for the security.
85
PEEK plumbing parts (Not included in Plumbing Parts Kits)

Description
Cat. No.
Male Nut 1.6MN-2 PEEK
228-33213
Male nutFerrule separation type
Contents
Ferrule 1.6F PEEK (includes 3pcs.)
228-33513-91
Male nutFerrule separation type
Male connector 1.6C PEEK
228-25014
Coupling
3way joint 1.6C PEEK
228-25013
Female T joint1
Stop joint PEEK
228-28090
Plug
Tubing cutter
228-32930-01
Press cutter for PEEK tube
Replacement blade
228-32930-02
PEEK Tube set
228-33406-91
Inline filter PEEK
228-32939
Frit (1pcs.)
228-32744
Spare filter for inline filter
Frit (5pcs.)
228-33691-91
Spare filter for inline filter
Rheflex fitting set
228-32651-41
Inc. nutferrule each 5

(A spare for Rheodyne 9725/i)2
Filter for protecting a column

(Put between injector and column)
PEEK tube (0.125 i.d.1.6 o.d.3000mm)
228-33833-91
PEEK tube (0.25 i.d.1.6 o.d.3000mm)
228-33833-92
228-33833-93
228-33833-94
Sample loop 100L
228-32651-16
Rheodyne injector for 9725/i
200L
228-32651-17
500L
228-32651-18
1mL
228-32651-19
1 Use this with chemical reaction detection system etc.

2 NutFerrule 5pcs. set for PEEK tube of Rheodyne 9725/i. Rheodyne 9725/i includes nut ferrule 4pcs. so that this set is not
necessary when installing.
"Rheflex" stands for Fex fitting made in Rheodyne.
86
Shimadzu Amino Acid Analysis kit for amino acid analytical system
As
both mobile phase and reaction solutions are

prepared to be optimized of analytical conditions, dilute
and pH adjusting is not necessary.
As strictly selected grade reagent is used, suitable for
high sensitive analysis.
Mobile phase kit
Analysis kit OPA reagent
Classification of analytical kit

Description
Cat. No.
Bottle name
AA-MA(Na)
Contents
0.2N-sodium citric acid
Capacity
1L
Usage
A solution (No.1 buffer)
228-21195-94
AA-MB(Na)
AA-MC(Na)

0.2N-sodium hydroxide
1L
500mL
B solution (No.2 buffer)
Amino acid mobile phase kit A solution 228-21195-96
AA-MA(Na)
AA-MA(Li)

0.15N-lithium citric acid
1L3pcs.
1L

Amino acid mobile phase kit Li type
AA-MB(Li)
AA-MC(Li)

0.2N-lithium hydroxide
1L
500mL
C solution (column clean up solution)
AA-MA(Li)
AA-RA
AA-RB

Basic buffer
O-phthalic aldehyde
1L3pcs.
500mL
500mL

Reaction reagent A
Reaction reagent B
Amino acid mobile phase kit Na type
228-21195-95
Amino acid mobile phase Li type A solution 228-21195-97

Amino acid analytical kit OPA reagent 228-21195-93
C solution (column clean up solution)
B solution (No.2 buffer)
Amino acid mobile phase kit Na type consists of mobile phase for amino acid analysis composed of protein (2 pcs.) and column clean up solution.
Amino acid mobile phase kit Li type consists of amino acid mobile phase and column clean up solution.
Analytical kit OPA reagent is a set of reaction reagent (2 kinds) and can be used in case of Na type and Li type.
Disposable Vial
Mass
products made by plastic

Suitable for the disposable of many samples
Septa is attached in a cap
Not necessary to set up septa
Special patterned ditch on the septa
The trouble such as the tears of the septa and the plugging
of the syring hole doesn't occur.
V bottom which solutions don't leave in
The amount of the sample is available in the range of 50l
to 1ml. Also, the shape of the bottom is arranged not to
leave air bubbles.
Specification
Injector
SIL-10A, SIL-9A, SIL-6B,

SIL-10AXL
Maxium amount
1mL
Minium amount of sample

(10l inject)
50
Materials
Polyethylene (Cap, Septa)

Polypropylene (Case)
Cat. No.
228-31600-91
Caution) When using disposable vial with a sample of organic solvent

type, be sure to check whether there are disturbing peak or not
to do branktest.
87
Disposable Filter
This disposable filter makes it possible to eliminates precipitate materials and minute particles in a sample. You can prevent
the plugging of the column, the change of the property, and the trouble of the injector and the micro syringe. Therefore the
column you use can be kept long.
Features
Small
disk shape disposable filter.

lock attached and you can filtrate with easily operation and no trouble.
The amount of the liquid left in this filter is quite small.
Filtration can be with high flow and low presure.
There are many kinds of filter including ion chromatography as follows.
Ruar
Series
Description
Aqueous type
Filter, 4A
Shape
Diameter (mm) Pore Size (m)
0.45
Cat. No.
670-12540-01
Features
Composed
of a hydrophilic
membrane and a
polypropylenehousing (blue).
Suitable
Filter,13A
13
0.45
670-12540-02
for the pretreatment of
the sample for HPLC analysis

and protein analysis which are
an object of aqueous and
alcohol solution.
Ion chromato type
Sterilization,
Filter,25A
25
0.45
670-12540-03
particles.
Filter,4AI
0.45
670-12540-11
Composed
eliminated
of a de-ionized
hydrophilic membrance and a

polypropylenehousing (light
blue)
Filter,13AI
13
0.45
670-12540-12
Suitable
the sample for ion

chromatography analysis.
As
Filter,25AI
25
0.45
670-12540-13
Filter,4N
0.45
670-12540-21
divided into each 10pcs,
clean can be kept.
Composed
of PTFE
membrance and a
Organic type
polypropylenehousing. (halftransparent)
Superiority
Filter,13N
13
0.45
670-12540-22
is solvent and
chemical compatibility
Suitable
semi-analytical sample which

are an object of solvent, strong
Filter,25N
25
0.45
670-12540-23
acid and strong alkarine.

It
is good for air vent filter.

(Inc. each 100pcs.)
88
Chemical Compatibility
Esters
Chemical name
Hydrogen
carbide
Ketones
Oil group Halogenation hydrogen carbide
Glacial acetate acid

Acetate acid 90
30
10
Hydrochloric acid Conc
6N
Silfuric acid Conc
6N
Nitric acid Conc
6N
Ammonia water 6N
3N
Calcium hydroxide 3N
Sodium hydroxide 5N
3N
Methanol
Ethanol
Propanol
Isopropanol
Butanol
Amyl alcohol
Ethylene glycol
Propylene glycol
Glycerine
Ethyl ether
Isopropyl ether
Dioxane
Tetrahydrofuran
Methyl esters
Etyl esters
Isopropyl esters
Butyl esters
Amyl esters
Serosolve esters
Organic type
Others
Ether
Alcohols
Alkaline
Acid
Chemical name
Can use Can use for short time
Aqueous &
Ion chromato type
Acetone
Cyclohexane
Metyl etyl ketone
Metyl isobutyl ketone
Benzene
Toluene
Xylenes
Etane dichloride
Methylene chloride
Chloroform
Carbon tetrachloride
Perchloroe etylene
Trichloro etylene
Freon TF
Freon TMC
Cotton-seed oil
Lubrication oil
Peanut oil
Sesame oil
Acetnitryl
Aniline
Gasoline
Kerosene
Dimethylsulfoxide
Dimethylsulfoxide
Terpene oil
Pyridine
Phenols (liquid)
Hexane (dry)
Formaldehyde 37
Aqueous &
Ion chromato type
Can NOT use
Organic type
Specification
4mm
Type
aqueous Ion chromato

type
type
Item
Pore size
4A
m
Organic
type

type
type
4N
13A
13A
25mm
Organic
type
13N

type
type
25A
25A
Organic
type
25N
0.45
0.45
0.45
0.45
0.45
0.45
0.45
0.45
0.45
OP
OP
PTFE
OP
OP
PTFE
OP
OP
PTFE
PP
PP
PP
PP
PP
PP
PP
PP
PP
818
818
818
1822
1822
1822
2924
2924
2924
0.07
0.07
0.07
0.8
0.8
0.8
4
4
4
7
7
7
5
5
5
6
6
6
100
100
100
100
100
100
100
100
100
Less than 10 Less than 10 Less than 10 Less than 30 Less than 30 Less than 30 Less than 100 Less than 100 Less than 100
Ruar lock Ruar lock Ruar lock Ruar lock Ruar lock Ruar lock Ruar lock Ruar lock Ruar lock
Filter
Housing
Size(L)
mm
Available filtration area
cm2
Maxium pressure
kg/cm2
Maxium temperature
Amount of the liquid left L

In
Connecting parts
Out
Ruar slip
Sterilization method2
E
Number(pcs.)/case
100
Stuff1
4A
13mm
Ruar slip
E
100
Ruar slip
A,E
100
Ruar slip
E
100
1 OPOlefines type polymer, PPPolypropylene, PTFEPolytetrafluoroethylene

2 SAUTO clave, EEthylene oxide gas
Ruar slip
E
100
Ruar slip
A,E
100
Ruar slip
E
100
Ruar slip
E
100
Ruar slip
A,E
100
89
6 Shim-pack Columns
Column series
Column name
Dimension
Cat.No.
Shim-pack Amino-Na
6.0mm i.d.10cm
228-18837-91
Shim-pack Amino-Li
6.0mm i.d.10cm
228-18837-91
Shim-pack CLC-ODST
4.6mm i.d.15cm
228-18062-91
Shim-pack WAX-1T
4.6mm i.d.5cm
228-18257-91
Shim-pack WAX-2T
4.6mm i.d.5cm
228-18258-91
Shim-pack WCX-1T
4.6mm i.d.5cm
228-18259-91
Shim-pack HPC-C2T
4.6mm i.d.5cm
228-18260-91
Shim-pack HPC-C3T
4.6mm i.d.5cm
228-18261-91
Shim-pack CLC-SIL
6.0mm i.d.15cm
228-00807-91
Shlm-pack CLC-SILM
4.6mm i.d.15cm
228-17872-91
Shim-pack CLC-SILM
4.6mm i.d.25cm
228-17872-92
Shim-pack CLC-ODS
6.0mm i.d.15cm
228-00808-91
Shim-pack CLC-ODSM
4.6mm i.d.15cm
228-17873-91
Shjm-pack CLC-ODSM
4.6mm i.d.25cm
228-17873-92
Shim-pack CLC-C8
6.0mm i.d.15cm
228-00809-91
Shlm-pack CLC-C8M
4.6mm i.d.15cm
228-17874-91
Shim-pack CLC-C8M
4.6mm i.d.25cm
228-17874-92
Shim-pack CLC-TMS
6.0mm i.d.15cm
228-00810-91
Shlm-pack CLC-TMSM
4.6mm i.d.15cm
228-17875-91
Shim-pack CLC-TMSM
4.6mm i.d.25cm
228-17875-92
Shjm-pack CLC-CN
6.0mm i.d.15cm
228-00810-91
Shim-pack CLC-CNM
4.6mm i.d.15cm
228-17876-91
Shim-pack CLC-CNM
4.6mm i.d.25cm
228-17876-92
Shim-pack CLC-Phenyl
6.0mm i.d.15cm
228-00812-91
Shim-pack CLC-PhenylM
4.6mm i.d.15cm
228-17877-91
Shim-pack CLC-PhenylM
4.6mm i.d.25cm
228-17877-92
Shim-pack CLC-NH2
6.0mm i.d.15cm
228-16725-91
Shim-pack CLC-NH2M
4.6mm i.d.15cm
228-17878-91
Shim-pack CLC-NH2M
4.6mm i.d.25cm
228-17878-92
Shim-pack CLC-VMA
6.0mm i.d.15cm
228-17255-91
Shlm-pack CLC-ODS/H
4.6mm i.d.25cm
228-00808-92
Shim-pack Amino
P.33
Shim-pack BIO T
Shlm-pack CLC
Reference
P.57
P.24
P.51
90
Column series
Column name
Dimension
Cat.No.
Shim-pack Diol-150
7.9mm i.d.25cm
228-14775-91
Shim-pack Diol-150
7.9mm i.d.50cm
228-14775-92
Shim-pack Diol-300
7.9mm i.d.25cm
228-14776-91
Shim-pack Diol-300
7.9mm i.d.50cm
228-14776-92
Shim-pack FLC-ODS
4.6mm i.d.5cm
228-13375-91
Shim-pack FLC-SIL
4.6mm i.d.5cm
228-13375-92
Shim-pack FLC-CN
4.6mm i.d.5cm
228-13694-91
Shim-pack FLC-C8
4.6mm i.d.5cm
228-13695-91
Shim-pack FLC-NH2
4.6mm i.d.5cm
228-13696-91
Shim-pack G-SIL4
4.0mm i.d.1cm
228-18270-91
Shim-pack G-SIL8
8.0mm i.d.1.5cm
228-18270-92
Shim-pack GK-SIL
30mm i.d.7.5cm
228-18338-91
Shrm-pack GL-SIL
50mm i.d.5cm
228-18339-91
Shim-pack G-ODS4
4.0mm i.d.1cm
228-18246-91
Shim-pack G-ODS8
8.0mm i.d.1.5cm
228-18246-92
Shim-pack GK-ODS
30mm i.d.7.5cm
228-18321-91
Shim-pack GL-ODS
50mm i.d.5cm
228-18322-91
Shim-pack G-C84
4.0mm i.d.1cm
228-18248-91
Shim-pack G-C88
8.0mm i.d.1.5cm
228-18248-92
Shim-pack G-TMS4
4.0mm i.d.1cm
228-18262-91
Shimpack G-TMS8
8.0mm i.d.1.5cm
228-18262-92
Shim-pack G-CN4
4.0mm i.d.1cm
228-18266-91
Shim-pack G-CN8
8.0mm i.d.1.5cm
228-18266-92
Shim-pack G-Phenyl4
4.0mm i.d.1cm
228-18264-91
Shim-pack G-Phenyl8
8.0mm i.d.1.5cm
228-18264-92
Shim-pack G-NH24
4.0mm i.d.1cm
228-18268-91
Shim-pack G-NH28
8.0mm i.d.1.5cm
228-18268-92
Shim-pack GPRC-SIL
8.0mm i.d.1.5cm
228-23462-92
Shim-pack GPRC-ODS
8.0mm i.d.1.5cm
228-23465-92
Shim-pack GPRC-C8
8.0mm i.d.1.5cm
228-24386-92
Shim-pack GPRC-TMS
8.0mm i.d.1.5cm
228-24387-92
Shlm-pack Diol
Shim-pack FLC
Reference
P.41
P.30
P.24, 29
Shim-pack G
P.54
91
Column series
Column name
Dimension
Cat.No.
Shim-pack GPRC-NH2
8.0mm i.d.1.5cm
228-24388-92
Shim-pack GPRC-CN
8.0mm i.d.1.5cm
228-24389-92
Shim-pack GPRC-SILK
3.0mm i.d.7.5cm
228-23462-93
Shim-pack GPRC-SILL
50mm i.d.5cm
228-23462-92
Shim-pack GPRC-ODSK
30mm i.d.7.5cm
228-23465-93
Shim-pack GPRC-ODSL
50mm i.d.5cm
228-23465-94
Shim-pack GPC-801
8.0mm i.d.30cm
228-20803-91
Shim-pack GPC-802
8.0mm i.d.30cm
228-20804-91
Shim-pack GPC-8025
8.0mm i.d.30cm
228-20805-91
Shim-pack GPC-803
8.0mm i.d.30cm
228-20806-91
Shim-pack GPC-804
8.0mm i.d.30cm
228-20807-91
Shim-pack GPC-805
8.0mm i.d.30cm
228-20808-91
Shim-pack GPC-806
8.0mm i.d.30cm
228-20809-91
Shim-pack GPC-80M
8.0mm i.d.30cm
228-20810-91
Shim-pack GPC-807
8.0mm i.d.30cm
228-20811-91
Shim-pack GPC-800P
4.6mm i.d.1cm
228-20812-91
Shim-pack GPC-801C
8.0mm i.d.30cm
228-20803-92
Shim-pack GPC-802C
8.0mm i.d.30cm
228-20804-92
Shim-pack GPC-8025C
8.0mm i.d.30cm
228-20805-92
Shim-pack GPC-803C
8.0mm i.d.30cm
228-20806-92
Shim-pack GPC-804C
8.0mm i.d.30cm
228-20807-92
Shim-pack GPC-805C
8.0mm i.d.30cm
228-20808-92
Shim-pack GPC-806C
8.0mm i.d.30cm
228-20809-92
Shim-pack GPC-80MC
8.0mm i.d.30cm
228-20810-92
Shim-pack GPC-807C
8.0mm i.d.30cm
228-20811-92
Shim-pack GPC-800CP
4.6mm i.d.1cm
228-20812-92
Shim-pack GPC-801D
8.0mm i.d.30cm
228-20803-93
Shim-pack GPC-802D
8.0mm i.d.30cm
228-20804-93
Shim-pack GPC-8025D
8.0mm i.d.30cm
228-20805-93
Shim-pack GPC-803D
8.0mm i.d.30cm
228-20806-93
Shim-pack GPC-804D
8.0mm i.d.30cm
228-20807-93
Shim-pack G
Shim-pack GPC
92
Reference
P.54
P.38
Column series
Shim-pack GPC
Shim-pack GRD
Column name
Dimension
Cat.No.
Shim-pack GPC-805D
8.0mm i.d.30cm
228-20808-93
Shim-pack GPC-806D
8.0mm i.d.30cm
228-20809-93
Shim-pack GPC-80MD
8.0mm i.d.30cm
228-20810-93
Shim-pack GPC-807D
8.0mm i.d.30cm
228-20811-93
Shim-pack GPC-800DP
4.6mm i.d.1cm
228-20812-93
Shim-pack GPC-2001
20.0mm i.d.30cm
228-23342-91
Shim-pack GPC-2002
20.0mm i.d.30cm
228-23342-92
Shim-pack GPC-20025
20.0mm i.d.30cm
228-23342-93
Shim-pack GPC-2003
20.0mm i.d.30cm
228-23342-94
Shim-pack GPC-2000P
8.0mm i.d.30cm
228-20812-94
Shim-pack GPC-2001C
20.0mm i.d.30cm
228-23343-91
Shim-pack GPC-2002C
20.0mm i.d.30cm
228-23343-92
Shim-pack GPC-20025C
20.0mm i.d.30cm
228-23343-93
Shim-pack GPC-2003C
20mm i.d.30cm
228-23343-94
Shim-pack GPC-2000CP
8.0mm i.d.30cm
228-20812-95
Shim-pack GRD-ODS
4.0mm i.d.25cm
228-16557-91
Shim-pack HPC-C2
4.0mm i.d.5cm
228-17792-91
Shim-pack HPC-C3
4.0mm i.d.5cm
228-17793-91
4.6mm i.d.15cm
228-23460-91
4.6mm i.d.25cm
228-23460-92
6.0mm i.d.15cm
228-23460-93
4.6mm i.d.15cm
228-24463-91
4.6mm i.d.25cm
228-23463-92
6.0mm i.d.15cm
228-23463-93
4.6mm i.d.15cm
228-24376-92
4.6mm i.d.25cm
228-24376-92
6.0mm i.d.15cm
228-24376-92
4.6mm i.d.15cm
228-24337-91
4.6mm i.d.25cm
228-24377-92
6.0mm i.d.15cm
228-24377-93
4.6mm i.d.15cm
228-24378-91
Shlm-pack HPC
Reference
P.38
P.50
P.47
Shim-pack HRC-SIL
Shim-pack HRC-ODS
Shim-pack HRC
Shim-pack HRC-C8
Shim-pack HRC-TMS
Shim-pack HRC-NH2
P.21
93
Column series
Column name
Dimension
Cat.No.
4.6mm i.d.25cm
228-24378-92
6.0mm i.d.15cm
228-24378-93
4.6mm i.d.15cm
228-24379-91
4.6mm i.d.25cm
228-24379-92
6.0mm i.d.15cm
228-24379-93
Shim-pack IC-A1
4.6mm i.d.10cm
228-17733-91
Shim-pack IC-A2
5.0mm i.d.5cm
228-17735-91
Shim-pack IC-C1
5.0mm i.d.15cm
228-17737-91
Shim-pack IC-GA1
4.6mm i.d.1cm
228-17734-91
Shim-pack IC-GA2
4.0mm i.d.1cm
228-17736-91
Shlm-pack IC-GC1
4.0mm i.d.1cm
228-17738-91
Shim-pack IC-PCI
8.0mm i.d.5cm
228-17744-91
Shim-pack IC-A1S
2.0mm i.d.15cm
228-33400-91
Shim-pack IC-A3S
2.0mm i.d.15cm
228-33366-91
Shim-pack IC-C3S
2.0mm i.d.10cm
228-33367-91
Shim-pack IC-CI PEEK
4.6mm i.d.10cm
228-33497-91
Shim-pack IC-GC1 PEEK
4.6mm i.d.9cm
228-33497-92
Shim-pack ISA-07/S2504
4.0mm i.d.25cm
228-09699-91
Guard Column ISA
4.0mm i.d.5cm
228-00823-91
4.6mm i.d.3.8cm
228-09700-91
4.0mm i.d.15cm
228-09328-91
4.0mm i.d.15cm
228-00796-91
4.0mm i.d.5cm
228-14206-91
4.0mm i.d.5cm
228-00821-91
Guard Column ISC-07
4.0mm i.d.5cm
228-00802-91
Guard Column ISC-07Li
4.0mm i.d.5cm
228-00797-91
Reference
Shim-pack HRC-NH2
Shim-pack HRC
Shim-pack-HRC-CN
Shim-pack IC
P.46
Shim-pack ISA
Shim-pack ISC
94
P.21
P.33
P.33
Column series
Column name
Dimension
Cat.No.
Shim-pack MBC-ODS
1.0mm i.d.25cm
228-12812-02
Shim-pack MBC-ODS
1.0mm i.d.50cm
228-12812-05
Shim-pack MBC-SIL
1.0mm i.d.50cm
228-12811-05
Shim-pack MBC-SIL
1.0mm i.d.100cm
228-12811-10
Shim-pack MBC-ACN
1.0mm i.d.25cm
228-12813-02
Shim-pack MBC-ACN
1.0mm i.d.50cm
228-12813-05
Shim-pack MBC-C8
1.0mm i.d.25cm
228-12814-02
Shim-pack MBC-C8
1.0mm i.d.50cm
228-12814-05
Shim-pack MRC-SIL
6.0mm i.d.25cm
228-23461-92
Shim-pack MRC-ODS
6.0mm i.d.25cm
228-23464-92
Shim-pack MRC-C8
6.0mm i.d.25cm
228-24381-92
Shim-pack MRC-TMS
6.0mm i.d.25cm
228-24382-92
Shim-pack MRC-NH2
6.0mm i.d.25cm
228-24383-92
Shim-pack MRC-CN
6.0mm i.d.25cm
228-24384-92
Shim-pack PA-DEAE
8.0mm i.d.10cm
228-20758-91
Shim-pack PA-DEAE
20mm i.d.10cm
228-20758-92
Shim-pack PA-QA
8.0mm i.d.10cm
228-20759-91
Shim-pack PA-QA
20mm i.d.10cm
228-20759-92
Shim-pack PA-CM
8.0mm i.d.10cm
228-20760-91
Shim-pack PA-CM
20mm i.d.10cm
228-20760-92
Shim-pack PA-SP
8.0mm i.d.10cm
228-20761-91
Shim-pack PA-SP
20mm i.d.10cm
228-20761-92
Shim-pack PAG-DEAE
8.0mm i.d.1cm
228-20762-91
Shim-pack PAG-QA
8.0mm i.d.1cm
228-20763-91
Shim-pack PAG-CM
8.0mm i.d.1cm
228-20764-91
Shim-pack PAG-SP
8.0mm i.d.1cm
228-20765-91
Shim-pack PRC-SIL
20mm i.d.25cm
228-23461-93
Shim-pack PRC-SILK
30mm i.d.25cm
228-23461-94
Shim-pack PRC-SILL
50mm i.d.25cm
228-23461-95
Shim-pack PRC-SILH
20mm i.d.25cm
228-23461-91
Shim-pack PRC-ODS
20mm i.d.25cm
228-23464-93
Shim-pack MBC
P.29
Shim-pack-MRC
P.21
Shim-pack PA
P.33
Shim-pack PAG
Shim-pack-PRC
Reference
P.33
P.21
95
Column series
Shim-pack-PRC
Column name
Dimension
Cat.No.
Shim-pack PRC-ODSK
30mm i.d.25cm
228-23464-94
Shim-pack PRC-ODSL
50mm i.d.25cm
228-23464-95
Shim-pack PRC-ODSH
20mm i.d.25cm
228-23464-91
Shim-pack PRC-C8
20mm i.d.25cm
228-24381-93
Shim-pack PRC-C8H
20mm i.d.25cm
228-24381-91
Shim-pack PRC-TMS
20mm i.d.25cm
228-24382-93
Shim-pack PRC-TMSH
20mm i.d.25cm
228-24382-91
Shim-pack PRC-NH2
20mm i.d.25cm
228-24383-93
Shim-pack PRC-NH2H
20mm i.d.25cm
228-24383-91
Shim-pack PRC-CN
20mm i.d.25cm
228-24384-93
Shim-pack PRC-CNH
20mm i.d.25cm
228-24384-91
Shim-pack PREP-SIL
20mm i.d.25cm
228-00814-91
Shim-pack PREP-SILH
20mm i.d.25cm
228-17880-91
Shim-pack PREP-SILHKit
4.6mm i.d.25cm
20mm i.d.25cm
228-17887-91
Shlm-pack PREP-ODS
20mm i.d.25cm
228-00815-91
Shim-pack PREP-ODSH
20mm i.d.25cm
228-17881-91
Shim-pack PREPODSHKit
4.6mm i.d.25cm
20mm i.d.25cm
228-17888-91
Shim-pack PREP-C8
20mm i.d.25cm
228-00816-91
Shim-pack PREP-C8H
20mm i.d.25cm
228-17882-91
Shim-pack PREP-TMS
20mm i.d.25cm
228-00817-91
Shim-pack PREP-TMSH
20mm i.d.25cm
228-17883-91
Shim-pack PREP-CN
20mm i.d.25cm
228-00818-91
Shim-pack PREP-CNH
20mm i.d.25cm
228-17884-91
Shim-pack PREP-Phenyl
20mm i.d.25cm
228-00819-91
Shim-pack PREP-PhenylH
20mm i.d.25cm
228-17885-91
Shim-pack PREP-NH2
20mm i.d.25cm
228-17879-91
Shim-pack PREP-NH2H
20mm i.d.25cm
228-17886-91
Shim-pack PREP-SILK
30mm i.d.25cm
228-18273-91
Shim-pack PREP-ODSK
30mm i.d.25cm
228-18319-91
Shim-pack PREP-SILL
50mm i.d.25cm
228-18274-91
Shim-pack PREP-ODSL
50mm i.d.25cm
228-18320-91
Shim-pack PREP
96
Reference
P.21
P.28
Column series
Shim-pack SBC
Column name
Dimension
Cat.No.
Shim-pack SBC-ODS
2.5mm i.d.15cm
228-17268-91
Shim-pack SBC-C8
2.5mm i.d.15cm
228-17269-91
Shlm-pack SBC-SIL
2.5mm i.d.15cm
228-17270-91
Shim-pack SCR-101N
7.9mm i.d.30cm
228-07730-92
Shim-pack SCR-101C
7.9mm i.d.30cm
228-17889-91
Shim-pack SCR-101P
7.9mm i.d.30cm
228-17890-91
Shim-pack SCR-101H
7.9mm i.d.30cm
228-07730-93
Shim-pack SCR-102H
8.0mm i.d.30cm
228-17893-91
Guard Column SCRN
4.0mm i.d.5cm
228-09619-92
Guard Column SCRC
4.0mm i.d.5cm
228-17891-91
Guard Column SCRP
4.0mm i.d.5cm
228-17892-91
Guard Column SCRH
4.0mm i.d.5cm
228-09619-93
Guard Column SCR-102H
6.0mm i.d.5cm
228-17924-91
Shim-pack SPC-RP3
4.0mm i.d.3cm
228-33713-91
Shim-pack SPC-RP2
4.6mm i.d.1cm
228-18838-91
Shim-pack SPC-AE1
4.0mm i.d.1cm
228-17990-91
Shim-pack WAX-1
4.0mm i.d.5cm
228-16225-91
Shim-pack WAX-2
4.0mm i.d.5cm
228-16365-91
Shim-pack WCX-1
4.0mm i.d.5cm
228-16366-91
Shim-pack SCR
Shim-pack SPC
P.30
P.41
Shim-pack WAX
Shim-pack WCX
Reference
P.49
P.33
P.33
97
7 Sample and data

Compound
AAA
ABA
ABA
Acenaphthene
Acetaldehyde
Acetaldehyde
Acetaminophen
Acetate
Acetic acid
Acetic acid
Acetic acid
Acetone
Acetylsalicylic acid
Acetylsalicylic acid
Aceumlnophen
Acrolein
Adenine
Adenosine
Adenosine
Adenylate kinase
ADP
ADP
ADP
ADP
AiBA
Ala
Ala
Ala
Ala
Ala
Ala
Ala
Ala
Albumin
Albumin
Amitriptyline
AMP
AMP
AMP
AMP
Amygdalin
AnBA
Ans
Arabinose
Arg
Arg
Arg
Arg
Arg
Arg
Ascorbic acid
Ascorbic acid
Asn
Asn
Asn
Asp
Asp
Asp
Asp
Asp
Aspartame
ATP
ATP
ATP
ATP
Atropine
Bacitracin
Benzaldehyde
Benzanilide
Benzene
Benzene
Benzoic acid
Benzoic acid
Benzoic acid
98
Page
Figure
37
79
37
16
18
71
77
73
43
72
73
18
26
78
26
18
25
25
32
44
18
35
74
78
37
37
37
71
74
76
79
37
79
44
48
16
18
35
74
78
75
37
37
43
37
37
71
73
76
79
27
68
37
71
79
37
37
71
74
76
17
18
35
74
78
25
61
18
21
21
40
17
68
72
51
164
51
3
11
117
153
126
64
122
130
11
21
155
21
11
16
16
34
66
6
42
132
158
51
50
51
119
137
149
164
51
164
67
76
3
6
42
132
158
143
51
51
63
50
51
119
137
149
164
27
113
51
119
164
50
51
119
137
149
4
6
42
132
158
17
104
11
54
4
114
123
Compound
Benzoic acid
Benzothorium Chloride
Berberine
Berberine
Big gastrin
Biotin
Biphenyl
Blue dextran 2000
Borate
Bovine scrum albumin
Br
Br
Bradykinin
Bromovalerylurea
Bromovalerylurea
BSA
Butanol
2butanone
Butylaldehyde
Butyraldehyde
Ca2
Caffeine
Caffeine
Caffeine
Caffeine
Caffeine
Caffeine
Caffeine
Caffeine
Caffeine
Calcium pantothenate
Calcium pantothenate
Camphor
Car
Carbamazepin
Carbamazepin
Carbonic anhydrase
Carbonic anhydrase
4Carboxybenzaldehyde
Cd2
CDP
CDP
Cellobiose
Chlorhexidine
Chlorhexidine gluconate
Chondroitin sulfate
Chrorpheniramine maleate
Chymotrypsin
Chymotrypsinogen A
Chymotrypsinogen A
Chymotrypsinogen A
Chymotrypsinogen A
Chymotrypsinogen A
Chymotrypsinogen A
Chymotrypsinogen A
Chymotrypsinogen A
Chymotrypsinogen A
Cinchonine
cisand translsoadhumulone
cisand translsocohumulone
cisand translsohumulone
Cit
Cit
Citrate
Citric acid
Citric acid
Cl
Cl
Cl
Cl
Cl
CMP
CMP
Co2
Page
Figure
74
75
18
75
60
68
72
44
81
36
46
82
61
26
32
68
60
18
71
18
46
17
18
25
26
32
68
72
75
77
68
72
76
37
72
78
36
58
81
46
35
78
37
18
76
76
26
44
35
36
36
36
48
48
58
61
64
61
51
51
51
37
79
73
43
72
46
73
77
81
82
35
78
46
135
141
9
142
96
113
124
65
175
45
70
179
104
21
37
115
94
11
117
11
72
4
7
13
21
37
113
120
138
153
113
120
144
51
121
154
45
90
173
73
42
158
49
8
146
147
18
65
38
46
47
48
74
76
91
101
107
100
80
80
80
51
164
126
64
122
70
126
152
174
177
42
158
73
Compound
Conalbumin
Creatinine
Crotonaldehyde
Cs
CTP
CTP
Cu2
Cys
Cysteine
Cystine
Cystine
Cytidine
Cytidine
Cytochrome C
Cytochrome C
Cytochrome C
Cytochrome C
Cytochrome C
Cytochrome C
Cytochrome C
Cytosine
1Decanol
Dehydroacetic acid
Dexamethasone
Dibutyl phthalate
Dibutylphthalate
Didecylphthalate
Diethyl phthalate
Diheptylphthalate
Dihydrocodeine phosphate
Dimethyl phthalate
Dioctyl phthalate
1Dodecanol
DOMA
DOPAC
Dulcoside A
Endorfin
Endorfin
Enolase
Ergosterol
Erythorbic acid
Estradiol
Estriol
Estrone
Ethanol
Ethenzamide
Ethenzamide
Ethinylestradiol
Ethofyline (I.S.)
Ethofyline (I.S.)
Ethosuximide
Ethosuximide
Ethyl acetate
Ethyl butyrate
Ethyl caproate
Ethyl propionate
Ethyl valerate
Ethylbenzoate
Ethylene glycol
Ethyleneglycol
Ethylparaben
F
FeEDTA
Fe2
Fe3
Fluocinolone acetonide
Folic acid
Formaldehyde
Formaldehyde
Formic acid
Formic acid
Fructose
Fructose
Fructose
Page
Figure
36
60
18
46
35
78
46
79
76
37
37
25
32
36
44
44
48
48
61
68
25
60
68
27
26
56
56
26
56
77
26
26
60
51
51
31
60
60
44
26
27
32
32
32
43
26
32
75
75
78
72
78
25
25
25
25
25
32
60
44
68
46
81
46
46
76
68
18
71
43
73
27
37
43
45
97
11
71
42
158
73
164
149
50
51
16
34
43
65
66
74
76
101
115
16
94
114
24
22
87
87
22
87
153
22
22
94
81
81
32
95
96
66
23
27
35
35
35
62
21
37
139
138
156
121
154
15
15
15
15
15
33
94
69
114
70
176
73
73
148
113
11
117
64
130
26
49
62
Compound
Fructose
Fructose
Fructose
Fructose
Fructose
Fumaric acid
Galactose
Galactose
Galactose
GDP
GDP
Giucose
Gln
Gln
Gln
Globulin
Glu
Glu
Glu
Glu
Glu
Glu
Glucose
Glucose
Glucose
Glucose
Glucose
Glucose
Glucose
Glucose
Glucose
Glucose
Glucose
Glutamate dehydrogenase
Gly
Gly
Gly
Gly
Gly
Gly
GlyGlyAla
GlyGlyLeu
GlyTyr
Glycerol
Glycerol
Glycerol
Glycyrrhrtinic acid
GMP
GMP
GTP
GTP
Guaninc
Guanosine
Guanosine
Hemoglobin A
Hemoglobin A
Hemoglobin C
Hemoglobin F
Hemoglobin F
Hemoglobin S
Hemoglobin S
Hesperidin
Hesperodine
Hexanaldehyde
Hexanol
Hexobarbital (I.S.)
His
His
His
His
His
His
Histamine
Homovanillic acid (HVA)
Page
Figure
65
65
73
73
73
43
37
43
73
35
78
37
37
71
79
64
37
37
71
74
76
79
27
43
43
43
65
65
65
65
73
73
73
44
37
37
71
74
76
79
61
61
44
27
43
43
77
35
78
35
78
25
25
32
35
36
35
35
36
35
36
17
74
18
60
78
37
37
71
74
76
79
79
79
109
111
127
128
129
64
49
63
129
42
158
49
51
119
164
108
50
51
119
137
149
164
26
61
62
63
109
110
111
112
127
128
129
66
50
51
119
137
149
164
103
103
65
26
62
63
150
42
158
42
158
16
16
34
39
44
39
39
44
39
44
5
133
11
94
154
50
51
119
137
149
164
163
161
99
Compound
Human serum albumin

HVA
Hydrocortisone acetate
HyLys
Hypoxanthine
Hypoxanthine
Hypoxanthine
Hypro
IgG
IgG
Ile
Ile
Ile
Ile
Ile
Ile
IMP
IMP
Inosine
Inosine
Insulin
Insulin
Insulin
Insulin B chain
Insulin b chain
isoButylparaben
isoMaltose
Isomaltoheptaose
Isomaltohexaose
Isomaltononaose
Isomaltooctaose
Isomaltopentaose
Isomaltose
Isomaltotetraose
Isomaltotriose
K
K
K
Ketoglutaric acid
Lactalbumin
Lactalbumin
Lactate
Lactate dehydrogenase
Lactic acid
Lactic acid
Lactoglobulin
Lactoglobulin A
Lactoglobulin B
Lactose
Lactose
Lactose
Leu
Leu
Leu
Leu
Leu
LeuEnkephalin
LeuEnkephalin
Levulinic acid
Li
lodoform
Lou
lrganox1076
Lys
Lys
Lys
Lys
Lys
Lys
LysBradykinin
Lysophosphatidylcholine (Lysolecithin)
Lysozyme
Lysozyme
Lysozyme
100
Page
Figure
44
51
27
37
18
74
78
37
44
58
37
37
71
73
76
79
18
74
18
74
60
60
61
61
60
68
73
65
65
65
65
65
65
65
65
46
46
82
43
36
58
73
44
43
73
44
36
36
27
37
65
37
37
71
73
76
61
61
43
46
76
79
81
37
37
71
74
76
79
61
25
35
36
36
65
81
24
51
6
132
157
51
67
92
50
51
119
137
149
164
6
132
6
132
95
96
104
104
95
114
129
112
112
112
112
112
112
112
112
71
72
178
64
45
90
126
66
64
130
65
45
45
26
49
109
50
51
119
137
149
103
104
64
71
145
164
172
50
51
119
137
149
164
104
12
38
46
47
Compound
Lysozyme
Lysozyme
Lysozyme
Lysozyme
Lysozyme
Lysozyme
Lysozyme
Lysozyme
Lysozyme
mtolualdehyde
Malate
Malic acid
Malic acid
Malic acid
Malonic acid
Maltoheptaose
Maltohexaose
Maltopentaose
Maltose
Maltose
Maltose
Maltose
Maltose
Maltose
Maltose
Maltotetraose
Maltotriose
Mannitol
Mannose
Mastoparan
1MeHis
3MeHis
Menthol
Mercaptoalbumin
Met
Met
Met
Met
Met
Met
MetEnkephalin
MetEnkephalin
Methacrolein
Methyiparabon
Methyl benzoate
Methyl benzoate
Methyl salicylate
Methylbenzoate
Methylephedrine
Mg2
MHPG
Mn2
Moltotriose
Myoglobin
Myoglobin
Myoglobin
Myoglobin
Myoglobin
Myoglobin
Myoglobin
Myoglobin
Myoglobin
Myoglobin
Myoglobin
Myoglobin
N1Acetylspermidine
N1Acetylspermine
Nacetylprocainamide
NAcetylputrescine
nButylbenzoate
nButylparaben
nHexyl benzoate
nPropyl benzoate
nPropylbenzene
Page
Figure
36
44
44
48
58
61
61
64
68
18
73
43
72
73
72
65
65
65
27
37
43
43
65
65
73
65
65
43
37
61
37
37
76
64
37
37
71
74
76
79
61
61
18
68
60
72
76
32
77
46
51
46
43
35
36
36
36
44
48
48
58
58
61
64
68
79
79
15
79
32
68
60
60
39
48
65
68
76
91
101
104
107
115
11
126
64
122
130
122
110
110
110
26
49
61
62
110
111
129
110
110
63
49
104
51
51
144
108
50
51
119
137
149
164
103
104
11
114
97
124
144
33
153
72
81
73
61
38
46
47
48
65
74
76
90
91
104
107
115
160
160
1
160
33
114
97
97
53
Compound
nPropylbenzene
nPropylbenzoate
nPropylparaben
Na
Na
Na
NAD
NADH
Naphazoline chloride
Naphthalene
Neostigmine sulfate
Neurotensin
NH4
NH4
NH4
NH3EtNH2
Ni2
Nicotinamide
Nicotinamide
Nicotinamide
Nicotinamide
Nicotinamide
Nicotinic acid
Nicotinic acid
Nicotinic acid
NO2
NO2
NO3
NO3
NO3
NO3
NO3
Nonmercaptoalbumin
Octanol
25OHD3
Orn
Orn
Ovalbumin
Ovalbumin
Ovalbumin
Ovalbumin
Ovalbumin
Ovalbumin
Ovalbumin
Oxine
Oxine
pBromoacetanilide
PEtNH2
pEtNH2
pHydroxy benzoic acid
pSer
pToluic acid
Paeoniflorin
Paeonol
Pantothenic acid
Pantothenic acid
PEG 1000
PEG 4000
PEG 4000
PEG 6000
Pentanal
Phc
Phe
Phe
Phe
Phe
Phenacetin
Phenobarbital
Phenobarbital
Phenol
Phenol
Phenylchalkone (I.S.)
Phenytoin
Phenytoin
Page
Figure
40
32
68
46
46
82
26
26
26
72
26
61
46
46
82
37
46
18
25
68
72
75
25
72
75
46
82
46
73
82
82
77
64
60
78
37
79
35
36
44
44
58
61
68
16
18
21
79
37
68
37
81
55
55
25
75
44
43
44
44
71
74
37
71
76
79
32
72
78
15
82
51
72
78
54
33
114
71
72
178
19
19
18
124
18
104
71
72
178
51
73
7
13
113
120
138
13
120
138
70
177
70
126
177
179
152
108
94
159
51
164
38
43
65
68
91
101
115
2
10
164
51
114
51
173
82
84
13
138
69
62
69
69
117
137
50
119
149
164
37
121
154
1
180
80
121
154
Compound
Phosphatidylcholine (Lecithin)
Phosphoric acid
Phridoxine
PO43
Polyethylene glycol
Polyethylene glycol
Polysaccharides
Polystyrene 1,100K
Polystyrene 1.35K
Polystyrene 115K
Polystyrene 33K
Polystyrene 410K
Polystyrene 7.6K
Polystyrene M.w. 9,120K
Polystyrene Mw 2,800
Polystyrene Mw 300
Polystyrene Mw 42,800
Pre
Primidone
Primidone
Pro
Pro
Pro
Pro
Pro
Pro
Propionaldehyde
Propionic acid
Propranolol
Propylaldehyde
Pullulan
Pullulan
Putrescine
Pyridoxine
Pyridoxine
Pyridoxine
Pyridoxine hydrochloride
Pyroglutamic acid
Pyroglutamic acid
Pyruvic acid
Quinine
Rafinose
Ramnose
Rb
Rebaudioside A
Rebaudioside A
Rebaudioside C
Rhamnose
Ribofiavin
Ribofiavin phosphate
Riboflavin
Riboflavin
Riboflavin phosphate
Riboflavin phosphate
Riboflavine
Ribonuclease
Ribonuclease A
Ribonuclease A
Ribonuclease A
Ribonuclease A
Ribonuclease A
Ribonuclease A
Ribonuclease A
Ribonuclease A
Ribonuclease A
Ribose
Saccharides
Saccharin
Salicylamide
Salicylic acid
Salicylic acid
Sar
Scopolamine
Ser
Page
Figure
25
43
18
46
63
63
43
39
39
39
39
39
39
39
40
40
40
37
72
78
37
37
71
74
76
79
18
72
16
71
63
63
79
25
72
75
68
43
73
43
61
65
65
46
31
74
31
37
72
72
25
68
18
25
75
61
35
36
36
36
44
48
58
64
68
37
42
25
26
68
78
37
25
37
12
64
7
70
105
106
61
53
53
53
53
53
53
53
54
54
54
51
121
154
50
51
119
137
149
164
11
122
3
117
105
106
160
13
120
138
113
64
130
64
100
111
111
71
32
134
32
49
120
120
13
113
7
13
138
101
38
46
47
48
65
74
91
107
115
49
60
14
21
114
155
51
17
50
101
Compound
Ser
Ser
Ser
Ser
Ser
SO42
SO42
SO42
SO42
Sorbic acid
Sorbic acid
Sorbic acid
Sorbic acid
Sorbitol
Sorbitol
Sorbitol
Sphingomyelin
Stevioside
Stevioside
Substance P
Substance P
Succinate
Succinic acid
Succinic acid
Succinio acid
Sucrose
Sucrose
Sucrose
Sucrose
Sucrose
Sucrose
Sucrose
Tartarate
Tartaric acid
Tau
Tau
Taurine
1Tetradecanol
Theanine
Theophyline
Thiamine
Thiamine
Thiamine
Thiamine
Thiamine
Thr
Thr
Thr
Thr
Thy
Thy
Thymidine
Thymine
Thymol
Thyroglobulin
Tocol
Tocopherol
Tocopherol
Tocopherol
Tocopherol
Tocopherol
Tocopherol
Tocopherol
Tocopherol
Tocopherol
Tocopherol
Tocopherol
Tocopherol
Tocopherol
Tocopherol
Tocopherol
Tocopherol
Transferrin
Transferrin
102
Page
Figure
37
71
73
76
79
73
46
77
82
25
68
72
74
43
43
73
25
31
74
60
61
73
72
73
43
27
37
43
65
65
73
73
73
72
37
79
75
60
74
78
18
25
68
72
75
71
74
76
79
37
37
32
25
76
44
32
27
31
32
73
27
31
32
73
27
31
32
73
27
31
32
73
44
48
51
119
137
149
164
126
70
152
177
14
114
123
135
62
63
128
12
32
134
95
104
126
122
130
64
26
49
63
109
111
127
128
126
122
51
164
140
94
136
156
7
13
113
120
138
119
137
149
164
50
51
34
16
144
65
36
25
31
36
131
25
31
36
131
25
31
36
131
25
31
36
131
65
74
Compound
Transferrin
Trp
Trp
Trp
Try
Trypsin inhibitor
Trypsin inhibitor
Tyr
Tyr
Tyr
Tyr
Tyr
Tyr
UDP
UDP
UMP
UMP
Undine
Unknown
Uracil
Uracil
Urease
Uric acid
Uric acid
Uridine
UTP
UTP
Val
Val
Val
Val
Val
Val
Vanillylmanderic acid (VMA)
Veleraldehyde
Vitamin A
Vitamin A acetate
Vitamin A palmitate
Vitamin B6
Vitamin B1
Vitamin B12
Vitamin B12
Vitamin B2
Vitamin D2
Vitamin D2
Vitamin D2
Vitamin D3
Vitamin E
Vitamin E
Vitamin E acetate
Vitamin K2
VLA
VMA
Xanthine
Xylose
Xylose
Xylose
yGlobulin
Zn2
Page
Figure
58
71
76
79
76
36
58
37
37
71
74
79
71
35
78
35
78
32
25
25
72
35
64
78
25
35
78
37
37
71
73
76
79
79
18
71
26
26
61
61
61
77
61
26
60
71
60
26
71
26
26
51
51
78
27
37
43
44
46
90
119
149
164
149
43
90
50
51
119
137
164
119
42
158
42
158
34
16
16
124
40
108
157
16
42
158
50
51
119
137
149
164
161
11
118
20
20
99
99
99
151
99
23
98
118
98
20
118
20
20
81
81
157
26
49
63
65
73
JQA-0376
Founded in 1875, Shimadzu Corporation, a leader in the

development of advanced technologies, has a distinguished
history of innovation built on the foundation of contributing to
society through science and technology. We maintain a global
network of sales, service, technical support and applications
centers on six continents, and have established long-term
relationships with a host of highly trained distributors located
in over 100 countries. For information about Shimadzu, and to
contact your local office, please visit our Web site at
www.shimadzu.com
SHIMADZU CORPORATION. International Marketing Division

3. Kanda-Nishikicho 1-chome, Chiyoda-ku, Tokyo 101-8448, Japan
Phone: 81(3)3219-5641 Fax. 81(3)3219-5710
URL http://www.shimadzu.com
The contents of this brochure are subject to change without notice.

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Shimadzu HPLC Columns

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C190-E043C

2 Guide for Selection of Operational Conditions

3 Shimadzu HPLC Columns

1 Classification of Shimadzu HPLC Columns

High stability in wide pH range

Silica Gel Type

Zorbax Series(ODS, C8, TMS, NH2, CN, SIL, etc.)

Shim-pack Series (Amino, ISC, ISA, SCR, IC, ION, GPC)

Shim-pack Series(PA, IC)

Asahipak Series(GS, ES, ODP)

Classification by Elution Mode

two groups are futher classified as follows:

Normal phase elution

NH2(Shim-pack, Zorbax) Saccharides, tocopherols

ODS(C18)(Shim-pack, Zorbax, CAPCELL PAK, STR)

Silica Gal Type

Reversed phase elution

Low molecular-weight compounds

Shim-pack WAX(Weakly basic anion exchange) Nucleotides,proteins

Shim-pack HPCProteins, nucleic acids

Shim-pack AFCProteins, nucleic acids

Reversed phase elution

Asahipak ODP-50(Polyvinyl alcohol)Peptides, proteins, low-molecular-weight compounds

Shim-pack Amino, ISC(Polystyrene)Amino acids, guanidines

Shim-pack IC(Polyacrylate, Polystyrene)Inorganic ions

Shim-pack SCR(Sulfonated polystyrene)Saccharides

Shim-pack SCR-101H, 102H(Sulfonated polystyrene)Organic acids

Asahipak GS(Polyvinyl alcohol)Water-soluble high polymers

Shim-pack GPC(Polystyrene)Synthetic high polymers, rubbers

Classification by Column Dimensions

(Representative Shim-pack series)

4.0mm i.d.15cm or 25cm

ISC series etc.

4.6mm i.d.15cm or 25cm

1.0mm i.d.25cm or 50cm

Large scale preparative

Micro bore columns

Small bore columns

SBC series small bore columns are 2.5mm in inner diameter.

FLC series fast LC columns are packed with 3m dia.

Classification by ODS Columns

Silica Gel Type

Analysis for general use

Analysis for general use

Analysis for general use

CAPCELL PAK C18

Analysis for general use

Analysis for general use

Analysis for general use

Polyvinyl alcohol gel

Method to Introduce C18 Groups

Selection of ODS Columns

Mobile phase pH range

End-capped to minimize the influence of residual silanol

Utilizes the influence of residual silanol compounds.

CAPCELL PAK C18

Silica gel solid support is coated with silicone polymer to

Applicable to mobile phase up to pH13.

2 Guide for Selection of Operational Conditions

cation exchange chromatography is most popular.

Biological amino acids

Protein-hydrolyzed amino acids

React before injects in columns. Special accessories are not needed.

Continuously add reaction solution to column elution.