Professional Documents
Culture Documents
Microbiology and Mycology
Microbiology and Mycology
INTRODUCTION
1. Microbiology and mycology
Microbiology and Mycology
By Prof. Jassiel Nyengani Zulu, University of Zambia, Department of Biological
Sciences, Lusaka and Dr. Modest Diamond Varisanga, Open University of Tanzania,
Faculty of Science, Technology and Environmental Studies.
3. Time
120 hours, will be required to complete this module; 80 hours for the part dealing with
Bacteria, Viruses and Protozoa and the remaining 40 hours for the part on Mycology.
4. Material
Access to the following materials is necessary to facilitate the study of this Module:
A hard copy (print) and electronic materials, including relevant CDs, CD-ROMS,
videos, and other related learning aids.
Relevant reference text books.
Laboratory equipment such as a microscope, an autoclave, oven, an incubator
(various temperatures), Petri plates, materials for bacterial and fungal media
preparations, various staining chemicals, identification manuals and other
laboratory equipment and materials for standard microbiology work
Capacity to facilitate for site visits/field trips.
5. Module Rationale
This Module provides a student with basic knowledge of the diversity of microorganisms.
These are; bacteria, viruses, protozoa and fungi. Their biology as it relates to their
economic importance in the environment are discussed in detail. The theory aspects of
the module will be elaborated through relevant laboratory exercises to illustrate the
principles and concepts of the subject matter. The knowledge attained will enhance the
teachers ability to teach High School Biology.
II. CONTENT
6. Overview
This Module is a study of organisms that can not be seen with the unaided eye unless
with the help of a microscope. They are referred to as microorganisms. More commonly
they are called microbes. These are bacteria, viruses, protozoa and fungi. Although
viruses are strictly not organisms, they too will be discussed under the same title for
convenience. Mycology which is the study of fungi includes some groups like the
mushrooms with macroscopic fruiting structures, which appear seasonally above the
ground. The study of microbes is called microbiology. The module starts with the history
of microbiology; the discovery of microbes and the development of sterile culture
techniques, and goes on to explore the diversity of microbes, their major biological
characteristics and economic importance. Examples of laboratory exercises meant to
familiarise the students with microbiological techniques such as, media preparation,
isolation, identification, culture maintenance, growth measurements, staining techniques
and preservation are presented wherever applicable.
Module Development Template
Outline
Table 1: Module Coverage and Lecture Schedule
Section Subsection Topic
6.1
History of Microbiology
6.2
Diversity of microbes
6.3
Hours
3
3
Practical
Self study
Compare electron (em) micrographs and
drawings of typical representatives of
each group
6.3.2
6.3.3
Bacteria
Structure, characteristics and
Classification
Nutrition
Growth and reproduction
6.3.4
Genetics
6..3.5
6.4.1
Economic significance
Isolation, identification and
culture techniques
Viruses
Structure and characteristics
6.4.2
Classification
6.4.3
6.4.4
Economic importance
6.3.1.
6.4
6.4.5
4
4
6
3
4
6
4
4
80
6.6.1
Fungi
Structure and characteristics
6.6.2
Classification of fungi
6.5
6.5.1
6.5.2
6.5.3
6.5.4
6.5.5
6.5.6
6.6
6.6.3
Identification
6.6.4
Nutrition
6.6.5
Growth
6.6.6
Reproduction
6.6.7
6.6.8
Ecology
Isolation, culture and
identification techniques
Sub total hours
4
4
types of fungi
Study visit to a research institutes for
identification demonstrations
Study the effects of nutritional
deficiencies on fugal growth
Watch a video demonstrating apical
growth in a typical fungus
Pictures and drawings of reproductive
structures
Study visit to a brewing industry
Video show
40
7. General Objective(s)
At the end of this Module, the learner should be able to;
7.1 describe the biology of microbes in terms of their structure and classification,
growth patterns and reproduction including their nutritional needs.
7.2 outline the economic importance of microbes.
7.3 describe the different techniques in the isolation, identification, and culture of
microbes.
7.4 apply the knowledge acquired from this course in the teaching of Biological
sciences to High School pupils.
8. Specific Learning Objectives (Instructional Objectives)
Unit 1: Bacteria
At the end of this unit, the learner should be able to;
1. describe how bacteria were discovered.
2. discuss the development of the culture technique.
3. classify bacteria based on their characteristics
4. explain the nutritional requirements of bacteria.
5. discuss metabolic activities of bacteria.
6. describe the conditions necessary for optimum growth of bacteria.
7. explain the genetics of bacteria.
8. explain the economic importance of bacteria.
9. demonstrate the techniques used to isolate and stain bacteria for.
Unit 2: Viruses
Module Development Template
After going through this unit, the learner should be able to;
1. describe the basic structure of viruses.
2. compare and contrast the structure of viruses with that of bacteria.
3. state the characteristics used to classify viruses.
4. list the taxonomic groups of viruses.
5. explain the process of viral reproduction.
6. perform biological techniques such as primary cell line preparation for viral culture,
plaque and quantal assays, methods of counting virions, and hemagglutination.
7. discuss the economic importance of viruses.
Unit 3: Protozoa
After completing this unit, the learner should be able to;
1. classify protozoa according to their taxonomic characteristics.
2. discuss the nutritional requirements of the protozoa.
3. discuss the physiology and metabolism of protozoa.
4. apply the culture technique used to grow protozoa.
5. explain the impact of pathogenic protozoa on humans.
Unit 4: Mycology
At the end of this unit, the learner should be able to;
1. classify fungi based on their characteristics.
2. describe the structure of a typical septate hypha of a fungus.
3. discuss the growth pattern of cellular and mycelial fungi.
4. explain the major types of nutrition among the fungi.
5. illustrate the major types of reproduction systems among the fungi using their life
Cycles.
6. explain the different modes of nutrition among the fungi.
7. explain the metabolism of carbon in fungi.
8. understand culture preservation techniques.
9. compare and contrast fungi with bacteria.
10. explain the economic importance of fungi.
Questions
1.
2.
http://en.wikipedia.org/wiki/Bacteria
Retrieved on 28th of March 2007.
3.
4.
5.
6.
http://en.wikipedia.org/wiki/Bacteria
Retrieved on 28th of March 2007.
The presence of
(a) nuclear material not contained in a membrane.
(b) a well defined flagellum.
(c) a cell wall.
(d) a plasma membrane .
8.
The titer of the virus, or the endpoint, is the dilution at which of the inoculated
hosts are affected or killed.
(a) 100%
(b) 80%
(c) 50%
(d) 10%
9.
10. Bacteria that obtain their energy from inorganic compounds containing iron,
nitrogen, or sulphur and not from decaying organic matter are
(a) chemolithotrophic.
(b) heterotrophic.
(c) autotrophic.
(d) parasitic.
11. The presence of viruses in the blood stream is referred to as
(a) cell tropism.
(b) a monocytic condition.
(c) viremia.
(d) viral contamination.
12. Each virus particle, is called a when it is on the outside of its host cell.
(a) virion
(b) capsid
(c) phage
(d) nucleoid
13. The shape of a virus is determined by the
(a) nucleocapsid that forms its head.
(b) type of nucleic acid it contains.
(c) shape of the capsid protein.
(d) number of nucleic acids in the cell
14. The protists are of medical importance because
(a) several cause diseases in humans.
(b) some are used in the manufacture of antibiotics.
(c) protists enter symbiotic relationships with many organisms.
(d) some are used for the manufacture of soap.
15. An example of the phylum Rhizopoda is
(a) Paramecium spp.
(b) Amoeba proteus.
(c) Plasmodium vivax.
(d) Escherichia coli
16. Which of the following pairs of words is mismatched?
(a) Plasmodium vivax malaria.
(b) Amoeba spp. severe diarrhea.
(c) AIDS Giardia lamblia.
(d) Escherichia coli tuberculosis
17. The phylum Sarcomastigophora
(a) consists of intracellular parasites.
(b) includes both flagellated and amoeboid parasites.
(c) is composed of ciliated parasites.
Module Development Template
http://en.wikipedia.org/wiki/ungus
Retrieved on 28/03/2007
The organism shown in the picture is .
(a) an Ascomycete.
(b) a Zygomycete.
(c) a Basidiomycete.
(d) an Oomycete
22.
The following picture illustrates blue cheese that is veined with a fungus called
Penicillium roquefori.
10
http://en.wikipedia.org/wiki/Ascomycota
Retrieved on 28/03/2007.
What message does the picture above get across?
(a) Fungi play an important role in the processing of food.
(b) Food infected by fungi is rendered unpalatable.
(c) All food types that are infected with a fungus go to waste.
(d) Cheese gets easily infected with fungi.
11
12
treatment plants, waste treatment plants, research centres, hospitals and the like. Your
study visit will be followed by submission of a report on the visit. Record your
observations and relate your experiences to the theoretical foundations from the literature
review.
Key concepts:
1
2.
Endospores: Highly resistant dormant structures formed by certain genera of Grampositive bacteria such as Bacillus, Clostridium, etc.
3.
Plasmids: Small DNA fragments found in some bacterial cells. Plasmids sometimes
carry genes for resistance to antibiotics.
4.
5.
6. Microscopy: Any technique that uses a microscope or any other tool for producing
visible images of structures or details too small to be seen by the human eye.
2.
3.
4.
13
5.
6.
7.
8.
http://www.wikipendia.org/wiki/Microbiology
http://en.wikipendia.org/wiki/Bacteria
http://gsbs.utmb.edu/bacteria/bacteria.html
http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookDiversity
2.html
9.
http://www.wikipendia.org/wiki/Microbiology
10.
http://www.stlcc.cc.mo.us/fp/users/kkiser/History.page.htm
List of optional readings:
1. Blake, L. et al. 2002. Biology 12. McGraw-Hill Ryerson Ltd.
2. Mader, S.S. 2004. Biology. McGraw-Hill Companies, Inc., 1221 Avenue of the
Americas, NY 10020.
3. http://www.cbs.dtu.dk/staff/dave/roanoke/genetics980309.html Accessed on
06/04/2007.
List of relevant useful links:
1.
2.
3.
4.
5.
6.
7.
http://www.slic2.wsu.edu:82/hurlbert/micro101/pages/101hmpg.html
http://www.ucmp.berkeley.edu/bacteria/bacterialh.html
http://en.wikipedia.org/wiki/Microscopy
http://en.wikipedia.org/wiki/Category:Laboratory_techniques
http://en.wikipedia.org/wiki/Category:Microbiology_techniques
http://en.wikipedia.org/wiki/Deer_Island_Waste_Water_Treatment_Plant
http:bact.wisc.edu/Microtextbook/index.php?module=Book&func=displayarticle
cle&a rt_id=25
14
15
http://en.wikipedia.org/wiki/Bacteria
Accessed on 19/03/08
Growth, which is an increase in the number of cells, is achieved by cell division. A
bacterial cell grows up to an optimum size and then duplicates by binary fission. This can
also be regarded as a means of asexual reproduction for bacteria. Given optimum
growing conditions bacteria can multiply exponentially.
The genetics of bacteria is centered on a single circular chromosome. Plasmids are extra
chromosomal DNA material that may contain genes for antibiotic resistance or indeed
factors for virulence.
Movements in bacteria take different forms. Bacteria use their flagella or pili for
movement. Motile bacteria can be attracted or repelled by external stimuli. An example
of this is chemotaxis whose details you cane look up under the following reference
(http://en.wikipedia.org/wiki/Chemotaxis)
Metabolism in bacteria is calssified on the basis of the type of energy (phototrophy, the
use of light through photosynthesis or chemotrophy, the use of chemical substances for
energy) used for growth, the type of carbon source (heterotrophic or autotrophic) used
and the type of electron donors (lithotrophs use chemical donors while organotrophs use
organic compounds donors) used for growth. Detailed reading can be accessed using the
following website; http://en.wikipedia.org/wiki/Bacteria
Bacteria can survive bad weather and other environmental hazards through the
development of endospores. They have a rigid and impermeable coat with cytoplasm,
which contains DNA and ribosomes at the center. They can lie dormant for millions of
years before they can become active again (http://en.wikipedia.org/wiki/Bacteria) under
favourable conditions.
The economic importance of bacteria includes both their useful and harmful aspects in
areas of medicine, agriculture and biological studies such as genetic engineering. You can
Module Development Template
16
http://en.wikipedia.org/wiki/Growth_medium
Accessed on 22/03/2008
An agar plate is an example of a bacterial growth medium. Specifically, it is a streak
plate. The orange lines and dots are colonies of bacterial growth.
17
18
Learning Activities
1.1 Readings: You are required to read the following references:
http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookDiversity 2.html
http://en.wikipendia.org/wiki/Bacteria
http://en.wikipedia.org/wiki/Microscopy
http://en.wikipedia.org/wiki/Category:Laboratory_techniques
http://en.wikipedia.org/wiki/Category:Microbiology_techniques
http://en.wikipedia.org/wiki/Deer_Island_Waste_Water_Treatment_Plant
Frobisher, M., Hinsdill, R. D., Crabtree, K. T. & Goodheart, C. R. 1974.
Fundamentals of Microbiology. Nonth Edition. Philadelphia: Saunders College
Publishing.
Any other Microbiology book, including those cited in this Module.
These readings will provide you with an overview on the historical perspectives and
discoveries that contributed to the study of microbiology, bacterial morphology,
classification, physiology and economic importance.
Write a brief summary of about 100 words on each of the following items:
1. Discoveries of microbes and microscopes.
2. The main features for classification of bacteria
3. Selective media for bacteria
4. Asexual and sexual reproduction in bacteria.
5. Bacterial Genetics
6. The importance of bacteria in the environment, food industry and agriculture
19
(http://www.slic2.wsu.edu:82/hurlbert/micro101/pages/101lab1.html (Accessed on
18/04/2007) for more information . Below is an example of a laboratory exercise:
Title: Culture of bacteria and their characteristics
You are required to take samples of water, soil, spoiled food including any such
environment of interest. Study the morphological diversity of bacteria using agar plates.
Use different staining techniques to identify the gram positive and gram negative
bacterial types.
Materials:
Microscope, slides, variety of nutrients, culture plates, incubator, clean bench, Pasteur
pipette, sample slides, alcohol, autoclave, Laminar flow cabinet, inoculating loops, source
of flame for sterilization of materials, cotton wool, mutton cloth, muslin cloth, different
bacterial stains.
Procedure:
Methods for the following activities should be learned; isolation, purification,
identification, staining and culture maintenance.
Report:
Depending on the type of task assigned, you may be required to prepare a report either
from a particular reading, laboratory exercise or a study visit. Usually the instructor will
advise you on the format of the report. However, most scientific reports use the following
format:
1.
2.
3.
4.
5.
6.
7.
8.
9.
Title: The title should be concise. It is a summary of the body of work that covers the
contents of the task under investigation.
Introduction: A brief statement of the background to the subject, its importance and
justification for the study.
Objective(s): A statement of the main expected outcomes (achievements) from the
study.
Methods: An explanation of the details of how the objectives are going to be
achieved. This is important because someone else should be able to use the
description of your methods in order to repeat the outcomes of your work
Results: Results are better presented in form of pictures tables, graphs, or charts.
These should carry a short and clear title, with a description of the results.
Discussion: An opportunity is provided for the interpretation of results focusing on
the objectives. Comparisons and similarities in the results with other published
works are discussed and acknowledged.
Conclusions: A statement is made as to whether the objectives of the exercise
had been achieved.
Recommendations: Suggestions are made about what needs to be investigated
further arising from some inconclusive results from the work.
References: all references used in the write up should be acknowledged in this part
using an accepted international format.
20
2.
Procedure: This will also depend on the objective of the visit itself
Report: You will be required to write a report on the activities of your study tour.
Whenever applicable, specific methods employed should also be described. Your report
should follow the same format described under report writing of the section under 1.2
titled Laboratory Practical.
1.4 Assignment:
1. Question: Write a brief essay (500-800 words) on the importance of bacteria in
medicine, agriculture and water quality.
0 Answer framework: In your essay, you may start with a description of what
bacteria are, their distribution in the environment and a discussion with given
examples of the effects bacteria have in the fields of medicine, agriculture, and
genetic engineering.
2.
1
21
dioxide production; or use of optical density). The other methods are those for
measurement of cell numbers (direct microscopic counts; electronic counting
chambers; and indirect viable cell counts or plate counts).
3.
Question: Explain, with the aid of a diagram, a typical bacterial growth curve.
Answer framework: A typical bacterial growth curve is represented in the figure
bellow. When bacteria are grown in a closed system (also called a batch culture),
like a test tube, the population of cells almost always exhibits these growth
dynamics. These are; the lag phase (A) when cells initially adjust to the new
medium until they can start dividing regularly by the process of binary fission, the
exponential phase (B) when the cell population grows logarithmically, the
stationary phase (C), when cell growth becomes limited by the presence of
secondary metabolites and or the depletion of nutrients in the growth medium and
the decline phase (D ) when cells stop dividing. Note the parameters of the x and y
axes. Growth is expressed as change in the number of viable cells over time. The
parameter that gets manipulated in an experiment, which is the variable is placed
along the x-axis while the outcome of the experiment is shown along the x-axis.
22
2.
3.
4.
5.
6.
7.
8.
Differentiate between:
Mesophile and thermophile.
Preservation and pasteurisation.
Sporulation and germination.
Selective medium and differential medium.
9.
10. Explain what occurs during each of the four phases of bacterial growth.
Learning Activity # 2
Title of Learning Activity: The Study of Viruses
Summary of the Learning Activity:
In this activity you will learn that viruses are clearly much smaller in size than most
bacteria. They possess characteristcs that are different from bacterial cells. While bacteria
are living organisms, viruses are non-living agents or particles that can infect all forms of
life, including members of the Bacteria, Archaea and Eukaryotes. Different viruses have
different shapes. Some are isometric or rod-like and others are helical. Viruses contain
either the ribonucleic acid (RNA) or deoxyribonucleic acid (DNA), but never both, hence
they are referred to as either DNA or RNA viruses. Virus classification is based on the
genomic structure (RNA or DNA), particle structure and the presence or absence of a
viral envelope. In this activity, you will learn about the different taxonomic groups of
viruses, their methods of cultivation, quantification (assay) techniques, their reproduction
and finally economic importance. A number of website references have been included for
your perusal. All of them deal with the structure and functions of viruses. Access the
Module Development Template
23
Drawing
Electron micrograph
(a) Isometric (adenovirus)
Drawing
Electron micrograph
(b) Helical (tobacco mosaic virus)
http://images.google.com/images?
gbv=2&svnum=10&hl=en&sa=X&oi=spell&resnum=0&ct=res
ult&cd=1& q=tobacco+mosaic+virus&spell=1
Accessed on 14/04/2007
24
Drawing
Electron micrograph
(c) Complex (T4 bacteriophage)
http://www.wikipedia.org/wiki/VirusStructure
Accessed on 14/04/2007
Visit the following site that will provide you with the Big Picture Book of Viruses. It can
be retrieved from http://www.virology.net/Big_Virology/BVFamilyGenome.html.
Key concepts:
1. Bacteriophage: Viruses that infect bacteria (phago means to eat). Bacteriophages
have been studied extensively since bacteria affected by them could be cultivated
much more readily.
2. Virion: A virus particle when it is on the outside of its host cell, and consists of
either DNA or RNA surrounded by a protective cover called capsid.
Module Development Template
25
3.
4.
5.
6.
Nucleocapsid: The viral capsid together with the nucleic acid that is tightly packed
within the protein coat.
Cytopathic effect: Are characteristic changes of the cells appearance observed in
tissue culture cells when they are attacked by viruses. It is one of the methods used to
identify viruses in cultured cells.
Titer: The titer of the virus, or the endpoint, is the dilution at which 50% of the
inoculated hosts are infected (ID50, infective dose) or killed (LD50, lethal dose).
Hyperplasia: Is excessive cell division or the growth of abnormally large cells,
resulting in the production of swollen or distorted areas of the organism.
26
27
http://images.google.com/images?gbv
Accessed on 14/04/2007
Learning Activities
2.1 Readings: For this activity you have to read the following references:
1.
28
29
30
31
Virus Replication
A virus attaches to the host cell and enters by endocytosis. The capsid protein dissociates
and the viral RNA is transported to the nucleus. In the nucleus, the viral polymerase
complexes transcribe and replicate the RNA. Viral mRNAs migrate to cytoplasm where
they are translated into protein. Then the newly synthesized virions bud from infected
cell.
http://en.wikipedia.org/wiki/Virus
accessed on 9/4/2008
32
3. For a virus to multiply, it must obviously infect a cell. Discuss this statement.
4.
5.
6.
7.
8.
9.
The following site provides valuable information regarding possible video material which
could be accessed to help answer some of the questions.
http://www.microbiologybytes.com/video/index.html (Retrieved on 02/04/2007).
Learning Activity # 3
Title of Learning Activity: The Protozoa
Summary of the Learning Activity:
This learning activity is going to cover another group of microorganisms known as
Protozoa. In this activity, you are going to classify protozoa and learn about their biology,
which will include nutrition, respiration, growth and reproduction, excretion and their
economic importance. Protozoa are unicellular organisms, which are usually found in the
Module Development Template
33
mud at the bottom of ponds and ditches. The contents of a protozoan cell are enclosed by
a thin limiting membrane. They have a simple structure comprising of a clear outer layer
called the ectoplasm and an inner more granular endoplasm. The endoplasm contains
organelles similar to those found in higher animals such as a true nucleus, mitochondria,
golgi apparatus, endoplasmic reticulum, ribosomes and vacuoles. They include both freeliving and parasitic forms. The parasitic protozoa inhabit both plant and animal hosts at
all phylogenetic levels, and the intracellular parasites can be found in almost all cell
types. The parasitic protozoans are microparasites because of their small size. They
divide within their definitive host cells and generally cause acute, rather than chronic,
diseases.
Key concepts:
1. Protozoology: A branch of biology, which deals with the study of protozoa.
2. Pseudopodia: Is a complex infrastructure of microtubules, microfilaments and other
organelles associated with the cell membrane in flagellated and ciliated protozoa.
3. Hyperparasitism (polyparasitism): A situation in which one organism which is a
parasite to another, is parasitized by yet another parasite of its own (i.e., a parasite
being parasitized).
4. Merogony (schizogony): Is a type of asexual reproduction in the phylum
Apicomplexa in which after nuclear division, the individual nuclei move to the cells
periphery and when nuclear division is completed, the cytoplasmic membrane then
surrounds each nucleus and the daughter cells bud from the parent.
Module Development Template
34
5. Bradizoite: A stage in the life cycle of protozoa of the family Sarcocystidae (tissuecyst-forming coccidia). In particular this is the term used to describe the merozoite
which forms within the tissue cyst in the intermediate host (and rarely within the
definitive host). This infectious stage rarely infects new cells within the intermediate
host; rather it is the infectious stage for the definitive host.
6.
Gametocyte: The stage in the life cycle of some protozoa (Apicomplexa) which is
destined to become a gamete (macro-or micro-).
http://en.wikipedia.org/wiki/Protozoa
http://en.wikipedia.org/wiki/Flagellate
http://en.wikipedia.org/wiki/Amoeboid
http://en.wikipedia.org/wiki/Sporozoans
http://en.wikipedia.org/wiki/Malaria#Distribution_and_impact
http://en.wikipedia.org/wiki/Cryptosporidiosis
http://en.wikipedia.org/wiki/Coccidia
http://en.wikipedia.org/wiki/Cryptosporidiosis
http://en.wikipedia.org/wiki/Toxoplasmosis
http://www.tulane.edu/~wiser/protozoology/notes/intes.html Intestinal Protozoa,
accessed on 19/04/2007.
11. Frobisher, M., Hinsdill, R.D., Crabtree, K.T. and Goodheart, C.R.1974.
Fundamentals of Microbiology. Sounders College Publishing, West Washington
Square, Philadelphia, PA 19105.
12. Nester, E.W. Anderson, D.G., Roberts (Jr.), C.E., Pearsall, N.N., and Nester, M.T.
2001. Microbiology: A Human Perspective. McGraw-Hill Companies, Inc.
List of optional readings:
1. Blake, L. et al. 2002. Biology 12. McGraw-Hill Ryerson Ltd.
2. Mader, S.S. 2004. Biology. McGraw-Hill Companies, Inc., 1221 Avenue of the
Americas, NY 10020.
List of relevant useful link:
http://www.who.int/mediacentre/factsheets/fs094/en/
Detailed description of the learning activity
In this activity you will learn that protozoa which belong to the Kingdom Protista
constitute a group of eukaryotic cells. They have a membrane-bound nucleus as well as
the other membrane-bound organelles that are characteristic of higher animals. Protozoa
are organisms that traditionally have been considered part of the microbial world. The
Module Development Template
35
36
through the ectoplasm. Another example of protozoa is the unicellular organism called
paramecium.
37
Learning Activities
3.1 Readings: Read the following books and links on6. Curd, C.R. 1992. Protozoa and
the water industry. Cambridge University Press, MA.
Protozoa:
1. http://en.wikipedia.org/wiki/Protozoa
2. http://en.wikipedia.org/wiki/Flagellate
3. http://en.wikipedia.org/wiki/Amoeboid
4. http://en.wikipedia.org/wiki/Sporozoans
5. http://en.wikipedia.org/wiki/Coccidia
6. http://www.tulane.edu/~wiser/protozoology/notes/intes.html
7. Cox, F. E. G. 2001. Modern parasitology, Blackwell Science
8. Curd, C.R. 1992. Protozoa and the water industry. Cambridge University Press, MA.
These readings will provide you with an overview of the morphology, classification,
reproduction, ecology and economic importance of protozoa. From these readings,
prepare your own brief summaries on the following:
1. Classification of protozoa (note the distinguishing characteristics of the major phyla:
Sarcomastigophora, Ciliophora, Apicomplexa and Microspora).
2. Ecology of protozoa. (Discuss the parasitic and nonparasitic forms using specific
examples to show how the environment supports their livelihood)
3. Growth and reproduction (types and life cycles, of both disease and non disease
causing protozoa).
4. Respiration (concentrate on how the organisms acquire their oxygen from the
environment to generate energy)
Module Development Template
38
5. Nutrition (take note of the mode of feeding and types of food source)
6. Excretion (note how the cells expel their waste to the environment)
7. Economic importance (especially in medicine, ecosystems and waste treatment).
3.2 Laboratory Practical:
Title: Diversity and morphology of protozoa
In this exercise you will be required to examine different samples (water, blood, stool,
etc.) for the diversity of protozoa and to learn about their morphology and life cycles.
Materials:
Microscope, samples, Petri dishes, and sample slides, saline solution.
Procedure:
To detect the presence of protozoa from humans and domesticated animals you should
collect stool and blood samples using recommended procedures. You will examine
different slides or real samples containing the protozoa. Make observations and compare
notes with what you read from various sources.
For
practical
microscopy,
you
may
visit
the
following
website:
http://www.micro.magnet.fsu.edu/primer/virtual/virtual.html where you will be given the
opportunity to do a virtual exercise. The site explains that: This interactive Java tutorial
explores the effect of increasing magnification (equivalent to changing microscope
objectives) on the ability to resolve features in a sample. We provide actual size images of
the sample so that students can understand the dramatic increases in magnification of the
samples they are examining. Each magnification is accompanied by a description of the
features available at that resolution. Samples featured in this module include computer
chips, Moon rocks, superconductors, and the surface of a Compact Disc.
Report:
As outlined in the Learning Activity # 1.
3.3 Study visits/Self Studies: Make a visit to a research station (agricultural or
livestock), or to a sewage treatment station if possible.
Objective: The objective(s) of such a study visit will vary according to the purpose.
Procedure: This will also depend on the objective of the visit itself.
Report: You will be required to write a report based on your records from observations
made during the study visit. Whenever applicable, specific methods employed should
also be described.
3.4 Assignment:
Module Development Template
39
Protozoa act as producers in both freshwater and saltwater ecosystems. They are part of plankton (Gr. plankt, wandering), organisms that are
suspended in the water and serve as food for heterotrophic organisms.
They enter symbiotic relationships ranging from parasitism to mutualism
(coral reef formation is greatly aided by the presence of a symbiotic
photosynthetic protists that live in the tissues of coral animals).
Polluted waters often have a rich and characteristic protozoa fauna. The
relative abundance and diversity of protozoa can be used as indicators of
organic and toxic pollution (i.e. used as environmental quality indicators).
Symbiont protozoa such as ciliates that inhabit the rumen and reticulum of
ruminants and the caecum and colon of equids are believed to aid the
animal in digesting cellulose.
Protozoa are employed as tools of study for various research projects;
because of their small size, short generation time and ease of maintaining
them in the lab.
2. Question: Malaria is one of the most important protozoa diseases. Malaria in man is
caused by a protozoan parasite of the genus Plasmodium. For an effective control
strategy, it is important to understand its life cycle. Describe the life cycle
of
Plasmodium spp.
Answer framework: See the following hints:
A]
The life cycle involves two stages of development: one, asexual, passed in
the human body; the other, sexual, passed on to the female of various species of
mosquitoes of the genus Anopheles.
B]
40
41
42
the yeasts to those that have visible fruiting structures such as the mushrooms. Fungi are
classified primarily by the type of spores and fruiting bodies they produce, although
molecular biology is beginning to take center stage in their taxonomy. Many mycologists
divide the Kingdom Myceteae (the Fungi) into five main phyla; the Chytridiomycota,
Zygomycota (zygospore fungi), Ascomycota (sac fungi) and the Basidiomycota (club
fungi). The fifth phylum called Deuteromycota (imperfect fungi) is used by some
taxonomists for fungi that apparently reproduce only by asexual spores.
Fungi are a member of a diverse group of eukaryotic organisms that unlike plants and
animals obtain food by absorbing nutrients from an external source. The majority of fungi
grow on and absorb food from substrates such as soil, wood, decaying organic matter as
heterotrophic feeders, while others are obligate parasites subsisting on nutrients derived
from living plant and animal tissues. Fungi are multinucleate, meaning that one cell can
contain two or more nuclei. They obtain their energy from respiration in a similar way
that higher plants and animals do. The outer layer of a fungal cell is a cell wall made of
chitin substance, followed by a cell membrane below which is a cytoplasm, which
contains all the organelles that are found in an animal cell. Unicellular fungi grow by
binary fission whereas the multicellular ones do so by extension growth of the apical
hypha. Fungal hyphae are collectively termed mycelium. Mycelia release their secondary
metabolites to the environment through the cell membrane. Fungi are so diverse in their
mode of reproduction to the extent that some reproduce exclusively by asexual means
while others combine the asexual and sexual modes of reproduction. And yet others
employ hormones in their sexual reproduction.
Fungi are both beneficial and a bother. From an ecological stand point, they are important
as decomposers and recyclers of nutrients in an ecosystem. Economically, fungi are a
source of food as many mushrooms are. They are also used in giving cheese their
different flavours. Fungi are also industrially important in baking, brewing and in
medicine as a source of antibiotics. In recent years fungi have become a focus of intense
study because of their use in biotechnological applications in processes related to
industry, agriculture ad ecology. Fungi are a concern because they damage millions of
dollars worth of farm produce through crop and animal diseases. Fungal infections in
humans are among the most chronic of illnesses.
43
Fungi help to break down dead organic material (in this case a
dead tree trunk) and help to continue the cycle of nutrients through
an ecosystem. http://www.ucmp.berkeley.edu/fungi/fungi.html
Accessed on 02/04/2007
Key concepts:
1. Mycology: The study of fungi, and a person who studies
fungi is known as a mycologist.
2. Hypha: A hypha (plural hyphae) is a long, filamentous cell of a fungus, constituting
the main mode of vegetative growth. A hypha consists of one or more cells surrounded
by a tubular cell wall. In most fungi, hphae are divided into compartments by internal
cross-walls
3. Septa: These are dividing cross walls along the length of a hypha. They usually leave
an opening at the centre called a septal pore. It is for this reason hyphae are said to
have compartments and not cells because no single compartment has protoplasm
exclusively to itself. The singular form of septa is septum.
4. Mycelium: A collection of hyphae of one fungus growing in one place is known as a
mycelium.
5. Gametangia: The organ of a fungus where gametes are produced. The singular of
gametangia is gametangium. A gametangium is a haploid structure and formation of
gametes does not involve meiosis
6. Monoecious fungi: Fungal species which produce male and female sex organs in the
same thallus (thus, hermaphrodite).
Module Development Template
44
7. Thallus: This is body of an organism that is not differentiated into roots, stem and
leaves.
8. Dioecious: Fungi that produce male and female sex organs on separate thalli.
9. Heterokaryosis: Heterokaryosis (from the Greek heteros, meaning other and karyon,
meaning kernel) means to have two or more genetically different nuclei within the
same mycelium of a fungus. The nuclei within the same mycelium may differ from
one another either through the accumulation of mutations or through the fusion of
genetically distinct hyphae. This can lead to individuals that have different nuclei in
different parts of their mycelium.
10. Dikaryotic hypha: A hypha that contains paired haploid nuclei.
11. Spore: A spore is a reproductive cell that develops into a new organism without the
need to fuse with another reproductive cell. Spores that are not contained in a capsule
are referred to as conidia.
12. Sporangium: This is a capsule that produces spores called sporangiospores.
13. Asci: This is a capsule that produces a special type of spore called ascospores
14. Basidia: This is a capsule that produces a kind of spore called basidiospores
http://en.wikipedia.org/wiki/Ascomycota 02/04/2007
http://www.kcom.edu/faculty/chamberlain/Website/Lects/Fungi.htm Lecturer notes
on Fungi. Accessed on 02/04/2007
http://pathmicro.med.sc.edu/mycology/myucology-1.htm. A book of Mycology.
Accessed on 02/04/2007
45
46
Teliomycetes, which comprise the rusts and the Ustomycetes, which embrace the smuts.
Phylum Deuteromycota: This group of fungi is also called the imperfect fungi. This is a
group of fungi whose sexual stage has not yet been discovered. As the sexual stage of a
fungus is discovered the fungus is removed from this group and gets its position in the
Ascomycota. The orders of this group are; Moniliales whose conidia arise on
conidiophores that are produced on mycelium (Alternaria tenuis), Sphaeropsidales whose
conidia are borne on conidiophores are produced in pycnidia (Phomopsis sp.),
Melanconiales whose conidia borne on conidiophores are produced in acervuli
(Pestalotia sp.), Sterlia which produce sterile mycelium and therefore no conidia are
produced, but other mycelial structures must be used for their propagation such as
scelerotia (Sclerotium sp.)
Phylum Lichens and Mycorrhizae: These are examples of beneficial associations
between two different organisms. In Lichens there is an association between an alga
(autotroph) with a fungus (heterotroph). The alga provides the fungus with a carbon
source while the fungus provides the alga with nutrients from the substratum. The same
applies to the association of fungi with plant roots when they form mycorrhizae. The
fungus provides the root system of a plant with mineral nutrients while the fungus gets
the carbon source from the plant in return. These are mutualistic associations.
Structure
Two structural forms exist among the fungi. One kind is unicellular as represented by the
yeast cells. The other form is made up of thread like structures. Individual threads are
known as hyphae whose singular form is hypha. Collectively hyphae are known as
mycelium whose plural form is mycelia. The mycelium is the vegetative phase of a
fungus, which subsequently gives rise to the reproductive structures. Whether it is a yeast
cell or a filamentous fungus consisting of hyphae, fungi are typically made up of a porous
outer cell wall made up of chitin, unlike the cell wall of plants that is made up of
cellulose. Inner to the cell wall is a cell membrane that is convoluted in places to increase
its surface area for exchange of materials. These structures are called lomasomes. The
cell membrane encloses the cytoplasm, which suspends the cellular organelles typical of a
eukaryotic cell. These are; a membrane bound nucleus, golgi complex, endoplasmic
reticula, ribosomes, and vesicles. Two types of hyphae are represented by different
groups of fungi. The lower fungi typically lack cross walls called septa whose singular is
septum. Such hyphae are said to be non-septate and therefore coenocytic because their
nuclei are contained in one continuous slug of cytoplasm. The hyphae of higher fungi
have septa, which divide the tubular filaments into compartments. However, the septa
leave a pore at the centre through which protoplasm can flow. Each compartment of a
hypha contains one to two nuclei. Vacuoles are characteristic of the older segments of a
hypha.
Nutrition
Fungi are achlorophylous, meaning that they do not contain chlorophyll to be able to
make their own food like plants do. Fungi depend on other organisms for their carbon
source. Therefore, they are heterotrophic feeders. Heterotrophs can either be saprobes,
symbionts or parasites. As saprophytes they obtain their carbon source from the byproducts of other organisms or from tissue of dead organisms as organic matter. When
Module Development Template
47
they are symbionts fungi usually live in close association with another dissimilar
organism in a mutual beneficial relationship. This kind of relationship is called a
mutualistic symbiosis. Other fungi are parasites in character. These are fungi that derive
their nutrition from the protoplasm of another organism called the host. Fungi have a
common mode of nutrition, which involves the release of enzymes on to the substratum
in the environment. The substrate is digested outside the cell and absorption of the
products (monomer forms) take place through the porous cell wall and the selectively
permeable membrane for eventual assimilation by the cells. Various types of enzymes are
produced depending on the type of fungus and the complexity of the substrate. A single
enzyme is required to digest for instance a disaccharide, whereas two enzymes will be
required to split a molecule of starch to its monomer units and yet three different types of
enzymes would be required to digest crystalline cellulose. This kind of digestion which
takes place outside the cell is called extracellular digestion. In addition to a carbon
source, fungi also need to take in mineral nutrients to supplement their metabolism, just
like higher organisms need the supply of nitrogen, potassium, phosphorus, etc.
Respiration
Fungi breakdown their carbon sources to release energy for metabolism in the
mitochondria like all eukaryotic cells do, through the following metabolic pathways;
glycolysis, Krebs cycle and electron transport chain is the main pathway used to release
energy for a cell.
Growth and development
The unicellular fungi grow by binary fission. The mycelial fungi grow by apical
extension. The apical compartment has a very thin cell wall at the tip, which allows the
apical compartment to extend in length due to internal cytoplasmic turgor pressure. After
attaining a maximum volume, the apical compartment under goes nuclear and
cytoplasmic division resulting into two compartments.
Reproduction
In fungi either sexual or asexual reproduction or both may occur by spore production,
often produced on specialised structures called conidiophores, which in turn bear conidia
or in sporangia, which contain sporangiospores, or variously shaped fruiting bodies such
as asci, which carry ascospores, or acervuli on which another kind of conidia are borne.
The kinds of reproduction methods are varied according to the type of fungus in
reference. However, asexual reproduction is the major means by which fungi reproduce
by either fragmentation of cells, binary fission or by budding. The other method of
reproduction in fungi is parasexual. Refer to the life cycles of the representative fungi
from the different taxonomic groups at the website given in the compulsory readings to
appreciate the different kinds of reproductive methods found among the fungi.
Significance
Fungi are important in the food industry. Mushrooms which are the macroscopic fruiting
structures of the Basidiomycota form a delicacy at the dining table in many countries. A
variety of species including Penicillium sp. are used to add flavour to cheese. The cheese
flavour would depend on the fungus species the cheese is inoculated with. The yeasts are
important in the leavening of dough in baking and the fermentation processes of wine and
beer manufacturing. In ecological systems, fungi are useful in the breakdown of organic
Module Development Template
48
matter and organic wastes and therefore contribute significantly to the recycling of
nutrients and cleaning up of wastes in an ecosystem. They are also an important tool in
research, because several generations of a fungus can be produced in a short time. The
field of medicine has benefited immensely from the exploitation of fungi for their
medicinal value. As mycorrhizal fungi they are known to have a mutualistic existence
with plants, in which plants enhance their phosphorus uptake, while the fungus benefits
from the carbon source provided by the host plant. On the other hand fungi are an
agricultural inconvenience because they cause disease on plants and animals, which cost
farmers billions worth of profits. A large number of fungi cause a variety of diseases in
plants, but only a few species cause disease in animals and humans, and when they do
they cause persistent illnesses.
Learning Activities
4.1 Readings: Read the following books and links on Mycology:
1.
2.
3.
49
Report: The format outlined in the learning activity No. 1 above should be followed.
Your report will now include representative drawings from the specimens observed and
this can be included under the results section of the report.
4.3 Study visits/Self Studies:
In order to achieve the objectives of this learning activity, you will need to organize
group or individual study tours to different places where fungi are commonly known to
grow, so that you can learn about their morphological characteristics, distinctive groups,
and their ecological environments. For the biological applications, you will need to visit
institutions and organizations that deal with mycological organisms. These may include;
bakeries, brewing factories, cheese making plants, wine and bio-ethanol producing
centres. Your report should include the names of fungi commonly used, the methods used
to exploit the fungi, and how inoculum is preserved. For each technology, report on the
process used to get the required results. Relate your experiences to the theoretical
foundations explored during the literature review.
Objective:
At the ed of the study visit the learner should be able to;
relate the theoretical knowledge to the practical experiences in the field.
Procedure: This will also depend on the objective of the visit
Module Development Template
50
Report: You will be required to write a report based on your observations from the
activities in the field. Whenever applicable, specific methods employed should also be
described.
4.4 Assignment:
As explained in the Learning Activity # 1.
Examples of Assignments:
1.
Question: Discuss the economic importance of fungi.
Answer frame work: Divide the response into the harmful and
beneficial aspects of fungi:
1.1
Harmful aspects:
Agents of disease
Candida albicans is a yeast which attacks the mucous membranes
causing infections of the mouth or vagina called thrush or
candidiasis; and also blamed for yeast allergies
Fungi like Epidermophyton cause skin infections but are not very
51
52
(A)
53
(B)
The Entomophthorales are an order of fungi that has traditionally been classified in the
class Zygomycetes. Most species of the Entomophthorales are pathogens of insects. The
name Entomophthorales is derived from the Greek for insect destroyer (Gk:
entomo=insect, phthor=destroyer)
http://en.wikipedia.org/wiki/Entomophthorales
Accessed on 8/4/2008
54
(C) Ringworm, also known as "Tinea" is an infection of the skin. Contrary to its name,
ringworm is not caused by a worm but by parasitic fungi (Dermatophytosis). It can exist
anywhere on the body.
http://en.wikipedia.org/wiki/Ringworm Accessed on 8/4/2008
55
56
http://en.wikipedia.org/wiki/Image:Baked_food.jpg
Accessed on 8/4/2008
Microorganisms that release carbon dioxide as part of their lifecycle can be used to
leaven products. Varieties of yeast are most often used, particularly Saccharomyces
species or baker's yeast. Leavening with yeast is a process based on fermentation,
physically changing the chemistry of the dough or batter as the yeast works. Yeast leaves
behind waste byproducts (particularly ethanol and some autolysis products) that
contribute to the distinctive flavor of yeast breads.
http://en.wikipedia.org/wiki/Leavening_agent#Biological_leaveners
2.
57
3.
Question: What are the ways in which fungi cause disease in humans?
Answer frame work: Fungi usually cause disease in humans in any one of the
following ways depending on the organism in question;
as an allergic reaction to inhaling fungal spores or vegetative cells causing
diseases such as hay fever and asthma, which is a disease of the respiratory
system caused by allergies with symptoms of coughing, sudden difficulty in
breathing, and a tight feeling in the chest.
as a reaction to the toxins produced by some species of fungi (some produce
toxins that are carcinogenic, e.g. Aspergillus produce aflatoxins, ergot from
Claviceps purporea, etc).
growth of fungi on or in the human body causing diseases or mycoses called
histoplasmosis, which is a severe disease of the lungs caused by the fungus
Histoplasma capsulatum; coccidioidomycosis, a respiratory disease of humans
and domestic animals caused by inhalation of spores from a fungus called
Coccidioides immitis; and candidiasis which are infections caused by yeasts such
as Candida albicans.
Fungi cause disease to plants destroying tones of crop yields per year. A shortage
of food in a household can lead to malnutrition.
4.
Question: Discuss the different kinds of symbiotic relationships fungi form with
other organisms. Explain how these relationships are beneficial to each partner.
Answer frame work:
There are two types of such relationships:
4.1 Lichen symbiosis
This is an association of fungi with photosynthetic organisms such as algae
or a cyanobacterium.
A fungus provides protection and a growth platform for the other organism.
The fungus supplies absorbed water and minerals to the partner organism.
the photosynthetic member supplies the fungus with organic nutrients as a
carbon source.
4.2 Mycorrhizae
These are close associations between fungi and the roots of certain plants
sustained by mutual beneficial effects between them.
Fungi increase the absorptive surface area of the root and an enhanced
58
2.
3.
4.
Describe the structure and function of each of the following parts of a fungus.
(a) Hyphal tip.
(b) Stolon.
(c) Rhizoids.
(d) Conidiophore.
5.
6.
7.
8.
9.
Search the internet for pictures of various organisms that belong to the Kingdom
Fungi. Provide a brief taxonomic description under each picture of a
representative organism for each of the following groups of fungi.
59
(a)
(b)
(c)
(d)
(e)
Chytridiomycota
Zygomycota
Ascomycota
Basidiomycota
Deuteromycota
10. Discuss the facts that fungi are a blessing as well as a curse.
11. COMPILED LIST OF ALL KEY CONCEPTS (GLOSSARY)
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
60
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
organism.
of bacteria.
Sarcocystidae
(tissue-cyst-forming coccidia).
stem and leaves.
HYPHA: A hypha (plural hyphae) is a long, filamentous cell of a fungus,
constituting the main vegetative part of a fungus.
MEROGONY (SCHIZOGONY): Is a type of asexual reproduction in the
phylum Apicomplexa in which a single cell undergoes several nuclear divisions
before the cytoplasm divides around them to form individual parent cells. The
malaria parasite reproduces rapidly in tissues of the host this way.
MICROSCOPY: Any technique for producing visible images of structures or
details too small to be seen by the naked human eye.
MONOECIOUS: Fungal species which produce male and female sex organs
in the same thallus and therefore assuming a hermaphrodite condition.
MYCELIUM: A collection of hyphae that form the vegetative body of a
fungus that is capable of feeding and reproducing.
MYCOLOGY: A branch of biology that specializes in the study of fungi.
NUCLEOCAPSID: The viral capsid together with the nucleic acid that is
tightly packed within the protein coat.
PLASMIDS: Small circular DNA contained in the cells of bacteria. They often
contain genes for antibiotic resistance. They are capable of independent
replication.
PROTOZOOLOGY: A branch of biology, which deals with the protozoa.
PSEUDOPODIA: Is a complex infrastructure of microtubules, microfilaments
and
other organelles associated with cytoplasmic protrusion used for
locomotion and or
feeding in protozoa.
SPORE: A reproductive cell that develops into a new organism without the
need to fuse with another reproductive cell.
TITER: The titer of the virus, or the endpoint, is the dilution at which 50% of
the inoculated hosts are infected (infective dose; ID50) or killed (lethal dose;
LD50).
TROPHOZOITE: A stage of development in protozoa in which the organism
is said to be mature and therefore active or able to feed.
VIRION: A virus particle when it is on the outside of its host cell, and consists
of either DNA or RNA surrounded by a protective cover called capsid.
Compulsory Reading # 1
Title: Microscopy
URL: http://en.wikipedia.org/wiki/Microscopy
Module Development Template
61
Abstract: Here you will find a description of the optical microscopy techniques such as
the bright field, oblique illumination, dark field, phase contrast, differential interference
contrast, and other related information. It includes a description of other types of
microscopy such as the electron and scanning probe microscopes.
Rationale: Microbes are too small to be seen by the unaided eye Therefore the study of
microbes requires knowledge of the use of tools like a microscope or other magnifying
instruments in order to appreciate the world of microbes.
Compulsory Reading # 2
Title: Microbiology and Bacteriology
URL: http://en.wikipendia.org/wiki/Bacteria
Abstract: Through this link, you will find an online textbook with chapters which deal
with different aspects of the biology of bacteria. The book includes topics on
classification and identification and the significance of microbes in biotechnology and
industry.
Rationale: By making use of this link, you will be able to appreciate how bacteria were
discovered, to learn about their diversity and biology. Given this knowledge you will then
be able to rationalize the role of bacteria in the environment and industry.
Compulsory Reading # 3
Title: Microbiology 102 Home Page
URL: http://en.wikipedia.org/wiki/Bacterial_growth
http://en.wikipedia.org/wiki/Microbial_metabolism
Description: These links take you to an online book of microbiology. From the table of
contents of this book, you will be able to get knowledge on three relevant topics in
bacteriology, namely: (a) Bacterial Growth http://en.wikipedia.org/wiki/Bacterial_growth
(b) Bacterial Nutrition (sources of energy, nitrogen, carbon, oxygen, etc), and (c)
Environmental Requirements for Microbes.
Rationale: The links will enable you accomplish part of the learning objectives in the
module; it is about bacterial nutrition, growth and environmental requirements for
growth.
62
Compulsory Reading # 4
Title: Laboratory Techniques
URL: http://en.wikipedia.org/wiki/Category:Laboratory_techniques
Abstract: This website will take you to a list of laboratory techniques which are
commonly used in microbiology. Click on the technique you wish to look up and that
website will take you to the link where the details of that technique are given.
Rationale: A prerequisite to the satisfactory study of microorganisms is their cultivation
under laboratory conditions; and in order to accomplish this, one must know some
techniques, particularly those that will enable the learner to study and manipulate
microorganisms in the laboratory away from their natural habitat.
Compulsory Reading # 5
Title: Viruses
URL: http://en.wikipedia.org/wiki/Virus#Discovery
Abstract: This link introduces another group of microbes, the viruses. It provides the
basic reading material on viruses; going as far as describing their discovery, origins,
classification, structure, replication, life forms, viruses and disease to include epidemics,
detection, purification and diagnosis, prevention and treatment, potential uses in therapy
and their applications.
Rationale: It has an extensive coverage on the part of virology.
Compulsory Reading # 6
Title: Protozoa
URL: http://en.wikipedia.org/wiki/Protozoa
http://en.wikipedia.org/wiki/Flagellate
http://en.wikipedia.org/wiki/Amoeboid
http://en.wikipedia.org/wiki/Sporozoans
http://en.wikipedia.org/wiki/Coccidia
http://www.tulane.edu/~wiser/protozoology/notes/intes.html
Abstract: These website references should be read together in order to have overview
knowledge about protozoa. Each web reference deals with a specific group of protozoa
explaining the classification and describing their biology. The significance of protozoa is
also discussed emphasizing their medical impact on human health.
Module Development Template
63
Rationale: The morphology and structure of the protozoa are well illustrated making the
descriptive text easy to follow. The life cycles of some protozoa are amply illustrated in
the following web reference: http://www.tulane.edu/~wiser/protozoology/notes/intes.html
Compulsory Reading # 7
Title: Fungi
URL: http://en.wikipedia.org/wiki/Fungus
Abstract: The link is packed with basics on fungi, which includes their characteristics
(morphologies, spores, etc.), fossil records and phylogenetic relationships. Detailed
descriptions of each of the major groups of fungi is covered.
Rationale: The link is a good introductory text on the study of fungi. It also has the latest
classification of the fungi based on the molecular approach.
64
URL: http://www.textbookofbacteriology.net/
Abstract: This link takes you to an online textbook whose contents are suitable for
general microbiology. It also covers relevant aspects of medical bacteriology with
specific examples of disease causing agents.
Rationale: It is important to widen your horizons on microbiology with an online
textbook that is at the cutting edge of knowledge in microbiology.
Useful Link # 2
Title: Bacteriology Techniques
URL: http://en.wikipedia.org/wiki/Category:Bacteriology_techniques
Abstract: This website will take you to a list of bacteriological techniques which are
commonly used in microbiology. Click on the technique you wish to look up and that
website will take you to the website where the details of that technique are given.
Rationale: A prerequisite to the satisfactory study of microorganisms is their cultivation
under laboratory conditions; and in order to accomplish this, one must know some
techniques, particularly those that will enable the learner to study and manipulate
microorganisms in the laboratory.
Useful Link # 3
Title: Malaria
URL: http://www.who.int/mediacentre/factsheets/fs094/en/
Abstract: This is a World Health Organization site which harbours updated information
on malaria. It defines what malaria is, the situation in Africa, its effects during pregnancy,
treatments and economic costs due to malaria.
Rationale: You should have noted so far that parasitic protozoans are able to survive
successfully and reproduce in almost all animal species. As such, protozoa are a
remarkably successful group of organisms that have evolved complex and closely
integrated relationships with their hosts. The study of Plasmodium spp. which causes
malaria, is a case example.
Useful Link # 4
Title: Microbiology and Immunology
URL: http://pathmicro.med.sc.edu/mycology/mycology-1.htm
Module Development Template
65
Useful Link # 5
Title: Mycological Resources on the Internet: Resources for Teaching
URL: http://mycology.cornell.edu/fteach.html
Abstract: It is a very rich source of different aspects of Mycology. Various topics are
covered. You will need to identify a particular topic and follow it up with the links
provided.
Rationale: It is important to know the classification of fungi, their structure and types of
associations, reproduction, ecological requirement and the usefulness of fungi. These are
the aspects covered by the various links, including mushroom cultivation.
66
2.
3.
67
5.
6.
7.
8.
9.
10.
A. Outline the steps involved in the lytic life cycle of a virus using the
bacteriophage as an example.
B. Provide an explanation for each stage cited.
11.
68
13.
14.
15.
17. References
2.
3.
4.
5.
6.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
Albach, R.A. and Booden, T. 1978. Amoebae. In: Parasitic Protozoa, Vol II
(ed.
J.P. Kriep). Academic Press. London.
Alexopoulos, C. J and Mims, C. W. 1996. Introductory Mycology, 4th Ed.
Carroll, G.C. and Wicklow, D.T. 1992. The Fungal Community: Its Organization
and Role in the Ecosystem. Marcel Deker, Inc., N.Y.
Cox, F. E. G. 2001. Modern parasitology. Blackwell Sciences.
Curd, C.R. 1992. Protozoa and the water industry. Cambridge University Press, MA.
Deacon, J.W. (2005). Fungal Biology (4th ed). Malden, MA: Blackwell
Publishers.
Frobisher, M., Hinsdill R. D., K., Crabtree T. and Goodheart, C.R. 1974.
Fundamentals of Microbiology. Ninth Edition. Philadelphia: Saunders College
Publishing.
Frobisher, M., Hinsdill, R.D., Crabtree, K.T. and Goodheart, C.R. 1974.
Fundamentals of Microbiology. Sounders College Publishing, West Washington
Square, Philadelphia, PA 19105.
Gelderblom, H.R. 1996. Structure and Classification of Viruses in Medical
Microbiology. Samuel Baron ed. University of Texas Medical Branch at Galveston.
Harkonen, M., Niemella, T. and Mwasumbi, L. 2003. Tanzanian Mushrooms.
Edible, harmful and other fungi. Botanical Museum, Finnish Museum of Natural
History, Helsinki.
Isaac, S., Frankland, J.C., Watling, R. and Whalley, A.J.S. 1993. Aspects of Tropical
Mycology. Cambridge University Press, Cambridge, UK.
Mader, S.S. 2004. Biology. McGraw-Hill Companies, Inc.. 1221. Avenue of the
Americas, NY 10020.
Michael M., John M. 2006. Brock Biology of Microorganisms, 11 th ed., Prentice
Hall.
Nester, E.W., Anderson, D.G., Roberts, E.C. (Jr), Pearsall, N.N. and Nester, M.T.
2001. Microbiology: A Human Perspective. McGraw-Hill Companies, Inc.
Radetsky, P. 1994. The Invisible Invaders: Viruses and the Scientists Who
Pursue Them. Backbay Books.
69
18. Authors
Principal Author of the Module
Prof. Jassiel Nyengani Zulu
Email address: [email protected]
Zambian and holder of a Ph.D. degree in Plant Pathology from Imperial College, London
University obtained in 1978. He is a lecturer at the University of Zambia and taught
ecology, evolution, plant biology, mycology and plant pathology to undergraduate and
trained postgraduate students since 1978 to date. He has reviewed curricula at both
undergraduate and postgraduate levels. Appointed to various administrative positions
within the University as Assistant Dean (undergraduate affairs), Head of Department of
Biological Sciences in the School of Natural Sciences and intermittently acted as the
Dean of the School of Natural Sciences. Currently he is Associate Professor in the
Biological Sciences.
70