Ester Synthesis Lab
Ester Synthesis Lab
AP Chemistry Laboratory
Introduction
An ester is a chemical compound that is formed when an organic acid reacts with an alcohol.
Esters frequently have distinctive odors and are naturally occurring flavor and fragrance chemicals
in many fruits and plants. In this experiment. the ester ethyl acetate (ethyl ethanoate) is prepared
and purified by distillation.
Concepts
Esters
Distillation
Reflux
Theoretical yield
Percent yield
Solvent extraction
Background
The reaction between an organic acid and an alcohol in the presence of an acid (H+) catalyst is
called esterification (Equation 1).
In the diagram, Rand R' represent organic groups such as hydrocarbons. The -OH group from the acid
combines with the -H atom from the alcohol to form a water molecule. The R' -0- group from the
alcohol attaches to the carbonyl carbon on the acid to produce the ester. The reaction is catalyzed by
the addition of concentrated sulfuric acid, H2S04, and the reaction is reversible. Adding concentrated
sulfuric acid, a strong dehydrating agent, shifts the equilibrium to the products side by removing the
water as it is formed.
Equation 2 illustrates a specific example of an esterification reaction, that of methyl alcohol and acetic
acid, to form methyl acetate. The systematic name for acetic acid is ethanoic acid, and the systematic
name for the ester product is methyl ethanoate.
Experiment Overview
In this experiment, a quantitative esterification reaction is performed. The process has three partsreaction, isolation, and purification.
In the reaction step, known amounts of acetic acid, ethyl alcohol, and sulfuric acid are combined and
heated under reflux. Refluxing a reaction mixture involves heating the mixture to its boiling point in a
flask equipped with a reflux condenser that allows a continuous return of the volatile materials to the
flask. Using a reflux condenser, the reaction is conducted at a higher temperature without losing reactants
or products.
Once the reaction is complete and the flask is cool, the ester is isolated and collected by the technique of
solvent extraction. A solution of saturated sodium carbonate is added to a separatory funnel along with the
contents of the reaction flask. These contents include any unreacted acetic acid, ethyl alcohol, and sulfuric
acid along with the ester and water. The saturated sodium carbonate solution is strongly basic (pH 10).
This basic solution wi1lconven any undissociated acetic acid to its salt (Equation 3).
Equation 3
Acetic Acid
Acetate Ion
By vigorously mixing this basic solution with the flask contents, the acetate ion along with the acidic and
polar components are extracted from the flask mixture and two immisible layers result. The top, or less
dense layer, contains the ester, while the bottom water layer contains sodium carbonate along with the
water-soluble components acetate ion, ethyl alcohol, and sulfuric acid.
The ester layer is transferred back to the flask and purified by the technique called distillation.
Distillation is the process of heating a compound to its boiling point and then removing the vapors by
cooling them with a condenser. The purified ester product is identified by its boiling point and its odor.
The condensed vapor, or distillate, is weighed, and the percent yield of ethyl acetate calculated.
Materials
Acetic acid, CH3COOH, 17.4 M, 12 mL
Hot plate
Ring stands, 2
Beaker,600-mL
Rubberbands
Beaker, 50-mL
Boiling stones
Capillary dropper
Clamps, 2
Three-way adapter
Safety Precautions
Concentrated sulfuric acid is severely corrosive to eyes, skin, and other tissue; use extreme caution
when handling. Ethyl alcohol is a flammable liquid and a dangerous fire risk; the addition of denaturants
makes ethyl alcohol poisonous. Acetic acid is corrosive to skin and tissue; it is a moderate fire risk. The
ester produced in this experiment, ethyl acetate, is a dangerous fire hazard; it is irritating to skin and eyes
and slightly toxic by inhalation, ingestion, and skin absorption. Use extreme caution when distilling
mixtures containing flammable liquids. Never smell chemicals directly by putting them under the nose.
Instead, hold the compound at least eight inches from the face with one hand, and use the other hand to
gently waft the vapors toward the nose. This lab should be performed in a fume hood or well-ventilated
area. Wear chemical splash goggles, chemical-resistant gloves, and a chemical-resistant apron. Wash
hands thoroughly with soap and water before leaving the laboratory.
Procedure
Preparation of ethyl acetate
1. Place 10 mL ethyl alcohol, 12 mL glacial acetic acid,
15 drops of concentrated sulfuric acid, and a boiling
stone in a 125-mL round bottom flask.
2. Place a 600-mL beaker, filled with approximately 450
mL of water and a few boiling stones, on a hot plate.
3. Place the round bottom flask in the beaker of water so
that the reaction mixture is below the water line. Clamp
the flask to a ring stand (Figure 1)
4.
4.
Purification of Ethyl Acetate
l. Add a boiling stone to the round-bottom flask.
2. Set up the distillation as shown in Figure 4. For all ground glass connections, lightly grease the
inner (male) joint to create a good seal.
3. Place the distilling flask in the 600 mL beaker and clamp as in the preparation step 3.
4. Place the three-way adapter vertically in the neck of the distilling flask.
5. Insert the thermometer into its adapter.
6. Place the thermometer and its adapter in the top of the three-way adapter so that the thermometer bulb is
just below the side arm.
7. Connect the condenser, with attached tubing, to the side arm of the three-way adapter and
clamp it to a ring stand.
8. Connect the outlet adapter to the condenser. Add rubber bands to secure the condenser
connections.
9. Weigh a clean, dry 125-mL Erlenmeyer flask on an analytical balance. Record the mass in the
Data Table.
10.Place the Erlenmeyer flask under the outlet condenser. Check all fittings to make sure all connections are
secure.
11.Attach the condenser inlet tubing to the water source and place the outlet tubing in the drain.
12. Slowly run cold water through the condenser.
13. Turn the hot plate on and heat the round-bottom flask in the hot water bath. Heat until the ethyl
acetate is gently boiling.
14. As the ethyl acetate vapors start to carry over and condense, record the temperature of the
vapors in the Data Table. Record this temperature at the beginning and end of the distillation in
the Data Table.
15. Distill the ethyl acetate until no more distillate comes over. There should be some liquid
remaining in the round-bottom flask. Never distill to dryness!
16. Turn off the hot plate.
Disposal
Dispose of the ethyl acetate as directed by your instructor.
Data Table
Boiling Point range ethyl acetate
___________________________C
___________________________C
___________________________C
_______________g
_______________g
_______________g
_______________g
_______________%
Fragrance
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