Mpat Complete Record
Mpat Complete Record
Mpat Complete Record
CERTIFICATE
Certified as a benefited record of practical work done by
Mr. /Miss
Regd. No.of ..B.Pharmacy / M.Pharmacy
in the Subject
.. during the year 2015-2016.
No. of Experiments done:
I/C LECTURER
PRINCIPAL
1
INDEX
Sino
DATE
PAGE
NO.
GRADE
SIGNATURE
OF LECTURER
100mg of paracetamol was weighed accurately and dissolved in 50ml of 0.1M NaOH and
then diluted it to 100ml with distilled water.
1ml of above solution was taken and diluted to 100ml with water.
From the above stock solution 6 standard dilutions are prepared by diluting 1ml, 2ml,
3ml, 4ml, 5ml, and 6ml with 0.1M NaOH to 10ml.
The concentrations that are obtained are 1/ml, 2, 3, 4, 5, 6g/ml and the absorbance was
measured by using 0.1M NaOH as blank.
The calibration curve was plotted between concentration and absorbance.
Assay:
20 tablets were taken and weighed accurately and the average weight the tablets were
calculated and made then into powder.
Equivalent weight of 0.15gms of paracetamol was weighed accurately and dissolved in
50ml of 0.1M NaOH and diluted it to 100ml with water.
Shake it for 5min and than filtered.
10ml of above filtered solution was taken and diluted to 10ml with distilled water.
From this 10ml was pipette out and 10ml of 0.1M NaOH was added to it and then diluted
to 100ml water and mixed well.
The absorbance of the solution was measured at 257nm against the blank solution.
Category: Analgesic.
Report: The percentage purity of paracetamol by calibration curve method was found to be
________
Procedure: Preparation of 0.1N NaOH: 4gms of NaOH was dissolved in 100ml of CO2 free
water. Which give 0.1N of the NaOH solution
Procedure for calibration curve:
10mg of the Ibuprofen was dissolved in 10ml of the 0.1N NaOH which gives the 1gm/ml
as a stock solution.
From the above solution, 2.5ml was taken into a 25ml volumetric flask and make up to
the mark using 0.1N NaOH which gives 100g/ml concentration.
From these solution 0.5ml, 5ml, 1.5ml, 2ml, 2.5ml was pipette out in 10ml volumetric
flask and finally the concentration was made up to the mark, which gives the
concentration of 5, 10, 15, 20, and 25g/ml.
The absorbance of the solution was measured at 264 nm using 0.1N NaOH as blank.
Assay:
100mg of pure drug was dissolved in 50ml of 0.1N NaOH solution and finally the
volume was made up to the mark with 0.1N NaOH which gives the concentration of
1mg/ml.
From the above stock solution 10ml was taken into 100ml volumetric flask to that 50ml
of 0.1N NaOH solution was added and the volume of the mark was made up with
distilled water.
0.5, 1.5, 2&2.5 of the above solution was taken into 10ml volumetric flask and is made
up to the mark with distilled water.
The concentrated of the resulting solutions are 5,10,15,20 & 25g/ml and the absorbance
were measured at 393nm and the calculation curve plotted.
Assay:
20 tablets were weighed accurately and the average weight was calculated and powdered.
150mg equivalent weight of the powder was weighed and dissolved in 100ml of 0.1N
NaOH.
From the above solution pipette out 1ml and dilute it to 10ml with 0.1N NaOH and
distilled water in1:1 ratio.
Procedure:
Preparation of standard dilutions:
100mg of diclofenac was weighed accurately and dissolved in little quantity of 0.1N
Noah and then diluted to 100ml with 0.1N NaOH.
10ml of the above solution was taken and diluted to 100ml with 0.1N NaOH.
From the above stock solution 5 standard dilutions are prepared by diluting 0.5ml, 1ml,
1.5ml, 2ml & 2.5ml with 0.1N NaOH to 10ml.
The concentrations that are obtained are 5,10,15,20 & 25g/ml and the absorbance were
measured by using 0.1N NaOH as blank.
The calibration curve was plotted between concentration and absorbance.
Assay:
Weigh and powder 20 tablets. Weigh accurately a quantity of powder containing about 50mg of
diclofenac sodium. Shake with 60ml of methanol in a 200ml volumetric flask and dilute it to
mark with methanol. Dilute 5ml of this solution to 100ml with methanol and measure the
absorbance of the resulting solution at the maximum at about 265nm.Calculate the content of
diclofenac from the absorbance.
Report: The percentage purity of diclofenac by calibration curve method was found to be
_________________
Liquid sample containing the element is aspirated into the flme for formation of liquid
droplets .
Evaporation of liquid droplets results in formation of salt residue.
The decomposition of residue to the formation of free neutral atomswhich are unstable
quickly emits photons & return to the lower energy state.
The measurement of emission radiation in terms of wave length or intensity on basis of
flame photometry.
If E1&E2 represents the energy of higher &lower energy state ,the radiation emitted during the
changing energy levels may be defined by equation .
E2 - E1= h
h=planks constant
hc
hc
= =
E 2E 1
We get E2-E1=
E2
Procedure: Prepare a series of standard solutions of 100ppm,200,300,400,500,600ppms
concentrations by taking sufficient amount of Nacl and dilute upto 100ml by using distilled
water. Switch on the flame photometer and select the sodium peak. Set the gas in flame ,in order
to get non-lumious flame & air pressure at 0.4-0.5 kg/cm2.Atomize the flame intensity to 100%
using standard solution of higher concentration. Measure the % flame intensity of all standard
solutions & unknown sample.
Report: The concentration of sodium ions present in the given solution was found to be
______________ by flame photometry.
Report: The absorbance of Potassium ion (K+) present in the given sample of KCl solution was
found to be __________ by flame photometry.
10
capillary tube. Hang the paper in a chromatographic chamber having the solvent. So that it
touches the surface of the solvent. Allow the solvent to run upto the 3/4 th of the paper. Remove
the chromatogram from the chamber and mark the solvent position with pencil. Dry the
chromatogram in air for 10 min and spray the dried chromatogram with Ninhydrin reagent. Dry
the chromatogram in an oven at 60C for 10-15 min. Purple colour spot will developed
components and the solvent front and compete the Fro values.
Report:
The Fro value of the given samples was found to be ______________ when the solvent system is
n-butanol: acetic acid: water 4:1:5.
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Procedure:
The prepared solvent system ( n-butanol : acetic acid : water in 4:1:5) was kept
aside for saturation of the chamber .
The redymade pre- coated plates were taken on which a straight line was drawn
above 2 inches from the edge of TLC plate.
Two points were marked equidistant on the straight line which were labelled as
A& B.
The TLC plate was placed in the saturated chamber for the development of the
chromatogram.
The spots were visvalated by spraying ninhydrin reagent followed by drying and
the florescent sample was observed under up- spectroscopy.
Report: The Fro value of the given sample was found to be______________.
15
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Interpretation of IR-Spectrum
Aim: To interpret the IR-Spectrum of 3-(N, N-diethyl amino) ethyl-2-thio-6-bromoquinazolin-4(3H) one.
Instrument: IR Spectrometer, Alpha-T-Broken.
Principle: IR Spectrum is the result absorption of light by vibrating molecules. In any
molecule it is known that atoms or groups of atoms are connected by bonds. These bonds
are analogues to springs and not rigid in nature. Because of continuous motion of the
molecule the maintain some vibrations with some frequency, characteristic to every
portion of the molecule. This is called natural frequency of vibration. When the energy in
the form of IR is applied and when applied IR frequency equal to natural frequency of
vibration absorption of IR taken place and peak is observed.
Procedure: Functional group analysis of the IR spectrum in Ker pellets. Here in the
spectrum we found NH functional group at 3378.89cm-1 as stretching vibrations.
We found in this spectrum, CH- aromatic group at 3185.24cm-1 as stretching vibration.
There is a C=O group found at 1671.43cm-1 as a stretching vibration.
The given spectra consist of a C-H group at 824.35cm-1 as a bending vibration.
Report:
The molecular formulae of the given drug which contains H atoms in 4- chemical
experiencing the electro negativity & anisotropic effect, so they resonate at 3.92.
The H of COOH group present at 2 nd position resonates at 10.48 due to the EN of
Report:
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