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Canola Oil Physical Chemical Properties 1
Canola Oil Physical Chemical Properties 1
PHYSICAL PROPERTIES
Selected physical properties for canola oil are shown in Table 1.
Table 1. Physical Properties of Canola Oil
Parameter
Value
0.914 - 0.917
1.465 - 1.467
67 - 70
78.2
Passed
220 - 230
275 - 290
1.910 - 1.916
0.179 - 0.188
Relative Density
The relative density of canola oil was first reported by Ackman and Eaton
in 1977 and later confirmed by Vadke et al. (1988) and Lang et al. (1992).
Noureddini et al. (1992) reported a density for high erucic acid rapeseed
oil of 0.9073 g/cm3 while Appelqvist & Ohlson (1972) reported a range
from 0.906 g/cm3 to 0.914 g/cm3. Ackman and Eaton (1977) indicated
that a different proportion of eicosenoic (C20:1) and C18 polyunsaturated
acids could be a major factor for the increase in relative density of canola
oil. The higher specific gravity of 0.9193 g/cm3 observed for soybean oil
can be attributed to the higher content of linoleic acid (Ackman and
Eaton, 1977). As for other liquids, the density of vegetable oils is temperature dependent and decreases in value when temperature increases
(Figure 1).
Crismer Value
The Crismer Value measures the miscibility of an oil in a standard solvent
mixture, composed of t-amyl alcohol, ethyl alcohol and water in the volume proportion 5:5:0.27. Crismer value (CV) is one of the specification
criteria used for international trade, mostly in Europe. Characteristic values are usually within a narrow limit (AOCS, 1992). The miscibility of an
oil is related to the solubility of glycerides, and is affected mainly by the
unsaturation and chain length of the constituent fatty acids. Little data is
available describing the solubility characteristics of canola oil.
Sahasrabudhe (1977) found that the Crismer value decreased from 82.0
to 76.8 with the reduction of erucic acid content from 54 to 0.1%.
Viscosity
Viscosity values estimate an oils relative thickness or resistance to flow.
Viscosity of refined, bleached and deodorized (RBD) canola is higher
than soybean oil (Figure 2).
Figure 2: Effect of Temperature on Viscosity of Canola and Selected Oils.
Adapted from Lang et al. (1992), Vadke et al. (1988)
and Noureddini et al. (1992)
Lang et al. (1992) and Noureddini et al. (1992a) found that the viscosity
of canola and other vegetable oils, like other liquids, was affected by
temperature and proposed an equation to calculate viscosity in the
temperature range from 4 to 100C. Figure 2 shows the relation
between temperature and viscosity for canola and selected vegetable oils.
Rapeseed oil exhibited a higher viscosity than canola, corn and soybean
oils. This can be directly related to the contribution of saturated fatty
acids (Noureddini et al., 1992a).
Smoke Point
Smoke point is the temperature at which a fat or oil produces a continuous wisp of smoke when heated. This provides a useful characterization
of its suitability for frying. The Canadian Government specifications
define that frying oil should have a smoke point above 200C. Table 1
indicates that canola oil fulfills this requirement. A similar smoke point
was observed for rapeseed oil (Appelqvist & Ohlson, 1972).
The heating technique used in the standard method for smoke point
determination is well-defined (AOCS Method Cc 9a-48). Arens et al.
(1977) reported that, when measured by different laboratories, the smoke
point for the same oil can differ by 10%, causing 20C deviation.
Therefore, caution should be exercised when comparing smoke points
reported from different laboratories. These variations are related to the
subjective determination by an observer as to when a continuous stream
of smoke occurs, and is not matched with a reference.
Flash Point
Flash point defines the temperature at which the decomposition products
formed from frying oils can be ignited (AOCS Method Cc 9b-55). This
temperature ranges from 275C to 330C for different oils and fats.
Canola oil falls within this range (Table 1).
Cold Test
The cold test measures the resistance of an oil to formation of a sediment
at 0C or 4C (AOCS Method Cc 6-25), and is generally used to measure
the effectiveness of the winterization process. Compounds with high melting temperatures, mainly waxes and triglycerides with saturated fatty
acids, usually cause sediment formation (Przybylski et al., 1993). The
cold test reveals whether an oil remains free of clouding when held at
4C or 0C for 15 hours. The formation of haze in canola oil is not a
common occurrence, but may happen on occasion (Mag, 1990). It has
been observed that oil produced from seeds grown in dry conditions will
develop sediment more quickly. This may be related to the higher content
of saturated fatty acids formed as a response to dry stress conditions
(Przybylski et al., 1993).
Fatty Acid
Linolenic (cis 9, 12, 15)
Linoleic (cis 9, 12)
Oleic (cis 9)
Octadecenoic (cis 6)
Elaidic (trans 9-octadecenoic)
Stearic
a
- Adapted from Mag (1990)
Polymorphism is a well-known phenomenon associated with the crystallization behaviour of long chain compounds. Fats can crystallize into
a number of sub-crystalline forms such as , , and ', each differing in
size and stability of the crystals (D'Souza et al., 1991). The ability of a fat
(Table 4). The solid fat indices of the oil phase blend must be in the range
of 25 - 30 at 10C, 14 -18 at 21.1C, and 2 -3.5 at 33.3C (Moustafa,
1992). The low solid fat index at 10C in soft margarines appeared to be
responsible for their spreadability at refrigeration temperatures (Table 4).
Formulation of these margarines requires the use of 70 -85% of slightly
hydrogenated and/or liquid oils, with a minimum of about 11 -15% of
highly hydrogenated stock (usually nonselective) (Table 4). Using this
stock, the formulated product is characterized by good spreadability
at refrigeration temperatures and good emulsion stability so there are
no oiling-out problems (Mag, 1990). Typical solid fat indices of soft
margarines are 8 - 14 at 10C, 5 - 8 at 21.1C, and 0.5 -2.5 at 33.3C
(Moustafa, 1992).
In margarines with good mouth-melt characteristics the oil phase in the
product melts sharply at body temperature which results in the total
breakdown of the emulsion in the mouth with the release of the flavourladen water phase (Moustafa, 1992). If the crystalline structure does not
melt rapidly the margarine feels waxy or thick in the mouth.
Table 3. Solid Fat Indices of Hydrogenated Canola Stocks and Margarines a
Sample
Solid Fat Index
Fatty acids
10.0C 21.1C 33.3C PUFA MUFA SAT.e TRANS
Hydrogenatedb
SHc
10.8
1.4
0.1
10.6
78.1
9.1
34.0
SH1
41.3
22.5
15.9
3.5
76.7 17.7
51.9
NSd
6.2
1.8
1.2
14.4
67.5 14.9
24.6
NS1
24.5
13.4
8.2
7.7
67.0 23.1
31.7
Margarines
Stick Regular
25 - 30 14 - 18 2 - 4
3 - 10 50 - 70 16 - 25
Stick Hi - Lif
16 - 24 10 - 15 1.5 - 4.0 20 - 40 20 - 50 13 - 23
Soft (70% Liq.)
8 - 14
5 - 8 0.5 - 2.5 30 - 60 15 - 42 10 - 20
CH2 0 R1
CH2 0 R1
CH2 0 R2
CH2 0 R2
CH2 0 R3
CH2 0 R3 FR
Acylglycerides
Phospholipids
CHEMICAL CHARACTERISTICS
Nature of Edible Oils and Fats
Edible oils and fats are composed primarily of triglycerides, which are
the ester of one molecule of glycerol and three molecules of fatty acids.
Canola oil analyses show that the triglycerides constitute 94.4 to 99.1% of
the total lipid (Mag, 1990). The typical composition of canola, rapeseed
and soybean oils is presented in Table 4.
Table 4: Constituents of Canola, Rapeseed and Soybean Oils a
Component
Triglycerides (%)
Phospholipids (%)
Crude Oil
Water-degummed
Acid-degummed
Free Fatty Acids (%)
Unsaponifiables (%)
Tocopherols (ppm)
Chlorophylls (ppm)
Sulfur (ppm)
Canola
94.4 - 99.1
Rapeseed
91.8 - 99.0
Soybean
93.0 - 99.2
up to 2.5
up to 0.6
up to 0.1
0.4 - 1.2
0.5 - 1.2
700 - 1200
5 - 35
3 - 15
up to 3.5
up to 0.8
0.5 - 1.8
0.5 - 1.2
700 - 1000
5 - 35
5 - 25
up to 4.0
up to 0.4
up to 0.2
0.3 - 1.0
0.5 - 1.6
1700 - 2200
Trace
Nil
Triglycerides
Triacylglycerols (TAG) are the most abundant lipid class found in canola
oil. The combination of fatty acids on the glycerol moiety is complex,
Fatty Acids
Fatty acids are composed of a carboxyl group and a hydrocarbon chain.
Individual fatty acids are distinguished from one another by the nature
of the hydrocarbon chain (Figure 5). This chain can vary in length from 4
to 24 carbon atoms and can be saturated, monounsaturated (one double
bond, MUFA) or polyunsaturated (two or more double bonds, PUFA).
The most common fatty acids in edible oils and fats are those containing
18 carbons. These include: stearic acid (a saturated fatty acid), oleic
acid (a monounsaturated fatty acid), and linoleic and linolenic acids
(polyunsaturated fatty acids containing two and three double bonds,
respectively) (Figure 5).
Figure 5:
Geometric Isomerism
In the case of unsaturated fatty acids, the carbon chain is bent into a
fixed position at the double bond, resulting in several possible geometric
isomers. When the portions of the chain are bent towards each other
they are called cis; and when bent away from each other, trans (Figure 5).
The natural configuration of fatty acids is cis, as shown for oleic acid.
The corresponding trans configuration, elaidic acid, results in a straight
chain (Fig. 5).
From a nutritional point of view the cis isomer is more desirable.
However, fatty acids with trans configuration affect the texture and
melting properties of fat or oil. Isomerization from cis to trans occurs
mainly during the hydrogenation of an oil. Formation of trans isomers
of linolenic and linoleic acids may also occur when harsh conditions are
applied during refining. During processing of canola oil formation of
trans isomers of linolenic and linoleic acids are observed. Oleic acid
is less prone to isomerization, trans isomers were detected only when
extreme parameters were applied. (Ferrari, 1996).
Due to elevated temperatures, deodorization is the stage of processing
where isomerization predominantly occurs. The effect of time and
Fatty
Acid
C10:0
C12:0
C14:0
C16:0
C18:0
C20:0
C22:0
Total
Saturated
4.0
1.0
1.0
0.8
0.1
3.9
1.2
0.6
0.4
0.1
3.4
2.5
0.9
0.5
0.1
38.8
4.1
2.7
1.6
0.4
0.2
0.1
6.3
4.1
0.1
0.1
0.1
10.8
4.0
-
6.2
4.7
-
11.4
1.9
-
6.0
6.9
6.2
7.4
47.9
10.4
14.9
10.9
13.3
0.2
60.1
1.4
0.2
0.3
15.0
10.0
45.1
0.2
61.1
1.5
0.1
0.2
76.8
1.6
0.1
0.2
32.8
0.8
0.5
0.1
16.5
0.1
-
0.2
23.8
0.2
-
0.2
20.4
-
0.1
25.3
-
61.9
70.1
62.9
78.7
34.3
16.7
24.2
20.6
25.4
C18:2n-6 20.1
C18:3n-3 9.6
Total
29.7
PUFAd
14.1
9.1
27.1
2.1
7.8
2.6
11.2
6.3
69.5
1.8
53.3
7.1
68.8
-
60.7
-
23.2
29.2
10.4
17.5
71.4
60.4
68.8
60.7
C16:1
C18:1
C20:1
C22:1
Total
MUFAc
Plant breeders developed canola oil with the linolenic acid content
reduced to 2.1% (Scarth et al., 1988) (Table 6). Storage stability
of this oil was shown to be better than regular canola oil (Przybylski
et al., 1993a). Low linolenic canola oil also exhibited improved frying
performance and better storage stability of fried products such as french
fries and potato chips (Petukhov et al., 1999; Warner and Mounts, 1993).
Canola has been further developed to produce an oil with an oleic acid
content raised from 60% to 85% (Wong et al, 1991). The fatty acid
composition of high oleic canola is presented in Figure 6. This oil showed
improved frying stability and produced better quality fried potato chips
(Petukhov et al, 1999). From the health and flavour formation point of
view, both low linolenic and high oleic canola oils should provide good
quality frying products without the presence of trans isomers (Ackman,
1990). Warner and Mounts (1993) found that some amount of linolenic
acid is required for good flavour formation in fried foods. This is due
to the formation of oxidation products, which are important flavour
compounds. Thus elimination of linolenic acid from oil can cause
negative changes in fried product flavour formation.
Recently canola oil with an elevated content of lauric acid was developed
(Table 6). This oil is being used in confectionery coatings, coffee whiteners, whipped toppings and center filling fats (Del Vecchio, 1996). Calgene
has also succeeded in the development of a canola plant that produces oil
containing 40% stearic acid. This oil could be used as a replacement for
hydrogenated fats in bread and bakery applications (INFORM, 1999).
Isomers
1
2
3
m
5
6
7
n
7
6
5
Polar Lipids
Sosulski et al. (1981) examined the polar lipids (PL) in several rapeseed
cultivars, including a low erucic acid (LEAR) winter cultivar grown
in Poland, and found that phospholipids formed the major component
(3.6 %) of the total polar lipids, while glycolipids contributed only 0.9%.
A more recent study by Przybylski and Eskin (1991) reported changes in
phospholipids during the early stages of canola oil processing (Table 7).
Table 7: Composition of Phospholipids in Canola Oil During Processing (%)
Oil Sample
Solvent
Expeller
Degummed
PEa
18.8
16.1
10.8
PIa
19.8
18.7
28.9
PAa
21.6
20.3
38.4
PSa
3.1
4.5
14.6
Oil
Canola WDGc
Canola PDGd
Soybean WDG
Soybean PDG
PC
32.5
39.2
32.5
29.1
PE
21.1
17.9
33.3
32.9
PI
15.2
12.6
17.3
16.4
LPCb
4.6
11.8
4.2
14.3
PA
3.2
1.1
3.5
1.8
PG+DPGb
23.4
17.5
9.2
5.6
Sosulski et al. (1981) examined the fatty acid composition of the individual phospholipids in the LEAR varieties from winter rapeseed cultivars
(Table 9). Smiles et al. (1988) found a similar fatty acid composition in
phospholipids from canola oil, with the exception of slightly higher levels
of linolenic acid (Table 9). Phosphatidyl choline contained the highest
amount of unsaturated fatty acids, mostly oleic and linoleic acids. The
other two phospholipids were rich in palmitic, linoleic and linolenic acids.
The presence of highly unsaturated fatty acids in phospholipids
is important as they can initiate oxidation of other fatty acids causing
accelerated deterioration of the oil. It was reported that phospholipids
have a tendency to complex heavy metals and in this form they are
a rather stable catalyst which can initiate and stimulate oxidation
(Pokorny, 1987).
Phospholipid
16:0 16:1 18:0 18:1
Phosphatidyl Choline
8.7
0.8
1.2
55.8
Phosphatidyl Inositol
21.8 0.8
1.9
33.6
Phosphatidyl Ethanolamine
17.7 1.8
2.0
47.7
a
Adapted from Sosulski et al. (1981) and Smiles et al. (1988)
Figure 8:
18:2
30.9
38.1
27.3
18:3
1.9
3.6
2.7
20:1
0.5
0.5
Trace Elements
The existing Codex standard for canola provides the maximum permitted
levels for iron, copper, lead and arsenic. While these metals are found
in other edible oils and are present naturally in the seed, they can be
introduced during handling and processing. Diosady et al. (1983) and
Elson et al. (1979) examined the effect of processing on trace elements
in canola oils. Their results are summarized in Table 10. These oils were
all of high quality with respect to cadmium and copper levels. It is clear
from the data in Table 10 that processing reduces the amount of toxic and
damaging trace elements, particularly lead, iron and sulfur. Iron in the oil
acts as a catalyst which can initiate free radical oxidation of unsaturated
fatty acids.
Table 10: Mineral Element Content in Canola Oilsa (ppm)
Oil Sample
Crude Oil
Degummed with
Water(WDG)
Phosphoric Acid(PDG)
Bleached
WDG
PDG
Deodorized
WDG
PDG
a
Phosphorus
1190.0
Iron
3.52
Calcium
296.0
Sulfur
6.5
Zinc
2.4
Lead
0.24
Isomers
R1
CH3
R2
CH3
CH3
H
R3
CH3
CH3
CH3
H
CH3
CH3
222.0
117.2
1.32
0.63
169.0
34.8
1.2
1.5
2.1
-
0.21
0.19
0.23
0.59
5.6
4.1
0.87
0.25
0.22
0.25
0.38
0.07
Tocopherols
The main nonsaponifiable components in vegetable oils are tocopherols
and sterols, which are present in varying amounts depending on the oil.
Tocopherols are natural antioxidants and their amount in the plant is probably governed by the content of unsaturated fatty acids. Tocopherols are
present in different isomeric forms (Figure 8).
Oil
HEAR
Canola
LLCanola
HOCanola
HOLLCanola
Soybean
Sunflower
Corn
LLFlax
P-8
268.0
272.1
149.8
226.3
285.8
116.0
613.0
134.0
25.8
0.1
34.0
17.0
18.0
-
426.0
423.2
313.6
201.6
607.2
737.0
18.9
412.0
212.6
7.1
2.7
8.2
275.0
39.0
9.2
96.8
74.8
46.5
42.2
82.5
129.3
Adapted from Zambiazi (1997) and Normand (1998). Abbreviations: HEAR - high erucic acid
rapeseed; LLCanola - canola oil with low content of linolenic acid; HOCanola - canola oil with high
content of oleic acid; HOLLCanola canola oil with low linolenic acid and high oleic acid; LLFlax
- flax oil with low content of linolenic acid; P-8 - Plastochromanol-8.
The content of tocopherols in RBD oils is affected by processing,
mainly by the extraction procedure and deodorization (Figure 9).