5 H
5 H
9, 2016
GEN CAMATO
HEMATOLOGY 2 LABORATORY
HEMATOLOGY 2
Pa#ent
Control
C1
C2
5mL
5mL
st
1 Set
5mL
5mL
nd
2 Set
P1 and C1
P2 and C2
Should incubate at Room
Incubate at Cold
temperature (19-22) aircon temperature (4) ref
* (both) Read for only 20 minutes
s After incubation we need to incubate both set at 37C
for 1 hour (Since our body is 37C so we need to look if there will be
RETICULOCYTE
Retics using Supravital stain.
It has Fine Reticulum of RNA
3)
GEN CAMATO
HEMATOLOGY 2 LABORATORY
HEMATOLOGY 2
A. Wet Method
1. New Methylene Blue Method Primary Use
2. Cook, Meyer, Tureen Method Brilliant Cresyl
Blue
3. Seivards method BCB *also categorized as Dry mtd
Methods of EOFT
B. Dry Method
1. Schillings rapid method BCB
2. Seiverds Method BCB
3. Osgood Wilhery Methylene Blue
4. Sabins Method Janus Green and Neutral
Red
1.
Sanford method
*Dry method - drop reagent in slide (thin film of rgt in slide), then drop of blood, cover
slip. Stand for 10min. focus under Oil Immersion Objective. Dry because it is thin smear.
*Most commonly used is the Wet method specifically the New Methylene Blue Method
Formula:
No.
of TT
#
% =
Normal value: 20 60 x 10
/mm3
%
=
Normal value: 24 84 x 10
24
23
22
21
20
19
18
17
16
15
14
D.
H2O
10
11
Bld.
0.36
0.34
0.32
0.30
0.28
/L
0.50
0.48
0.46
0.44
0.42
0.40
0.38
Interpretation of Results
*in 1 field there should be 250 RBCs; count usually in equal distribution. We dont need
to count 1 by one for rbc count.
= # .
Interpretation:
No hemolysis
250
250
250
250
Initial hemolysis
Complete hemolysis
2
25
.50%
NaCl
Methods of Reticulocyte
GEN CAMATO
HEMATOLOGY 2 LABORATORY
HEMATOLOGY 2
Eosinophil count
2.
3.
4.
5.
Increase OFI
1. Hereditary spherocytosis
incase of Hemolytic anemia
Decrease OFI
1. Sickle cell
2. Sideroblastic anemia
3. Thalassemia *Target cells
4. Jaundice
Methods of Eosinophil
Reagents
1)
2)
3)
4)
GEN CAMATO
HEMATOLOGY 2 LABORATORY
HEMATOLOGY 2
Counting Basophil
Procedure
Formula
=
Thorn test
Hyperadrenalism
Cushings disease
(decrease eosinophil)
* adrenal gland (above of kidney);
responsible for releasing Aldosterone
substance. Require for Sodium reabsorption;
associated with Hypercorticolism
Hypoadrenalism
Addisons disease
(increase eosinophil, but there is
an absence of eosinophilic
response)
Thorn procedure
12 hours fasting
Collect blood and get the base line eosinophil count
Inject the 25mg of Adrenocorticotrophic hormone
(ACTH) *Stimulate by Pituitary gland, then wait for 4 hours
After 4 hours, collect the blood and count again the
number of eosinophil
Interpretation