Coulter ACT DIFF
Coulter ACT DIFF
Operators Guide
TM
diff
READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL BEFORE ATTEMPTING
TO OPERATE INSTRUMENT. DO NOT ATTEMPT TO PERFORM ANY PROCEDURE BEFORE CAREFULLY READING ALL
INSTRUCTIONS. ALWAYS FOLLOW PRODUCT LABELING AND MANUFACTURERS RECOMMENDATIONS. IF IN DOUBT AS
TO HOW TO PROCEED IN ANY SITUATION, CONTACT YOUR BECKMAN COULTER REPRESENTATIVE.
BECKMAN COULTER, INC. URGES ITS CUSTOMERS TO COMPLY WITH ALL NATIONAL HEALTH AND SAFETY
STANDARDS SUCH AS THE USE OF BARRIER PROTECTION. THIS MAY INCLUDE, BUT IT IS NOT LIMITED TO,
PROTECTIVE EYEWEAR, GLOVES, AND SUITABLE LABORATORY ATTIRE WHEN OPERATING OR MAINTAINING THIS OR
ANY OTHER AUTOMATED LABORATORY ANALYZER.
CAUTION System integrity might be compromised and operational failures might occur if:
r This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals.
r You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your systems
computer with software authorized by Beckman Coulter.
r You install software that is not an original copyrighted version. Only use software that is an original copyrighted
version to prevent virus contamination.
IMPORTANT If you purchased this product from anyone other than Beckman Coulter or an authorized Beckman Coulter
distributor, and, if it is not presently under a Beckman Coulter service maintenance agreement, Beckman Coulter cannot
guarantee that the product is fitted with the most current mandatory engineering revisions or that you will receive the most
current information bulletins concerning the product. If you purchased this product from a third party and would like
further information concerning this topic, call your Beckman Coulter Representative.
REVISION STATUS
Issue B, 12/97
Software Version 1.03
Table 6.1 was changed to reflect updated information for replacing diluent filters and
peristaltic pump tubing. A procedure for cleaning the inside of the instrument was added to
Additional Cleaning Procedures. The first paragraph of Headings 6.8 and 6.9 was changed.
Pages changed: 6-2, 6-7, 6-25 and 6-29.
Issue C, 11/99
Software Version 1.06
Note: A black bar in the left margin indicates where a change was made from the previous
version of the manual.
Note: A black bar in the left margin indicates where a change was made from the previous
version of the manual.
Page Change
This document applies to the latest software listed and higher versions. When a subsequent software version
changes the information in this document, a new issue will be released to the Beckman Coulter website. For
labeling updates, go to www.beckmancoulter.com and download the most recent manual or system help for
your instrument.
PN 4237416DB 3
REVISION STATUS
Page Change
4 PN 4237416DB
REVISION STATUS
Issue D, 6/03
Changes were made to,
r comply with the EU IVD Directive (98/79/EC).
r change the company name from Coulter Corporation to Beckman Coulter Inc.
Issue DA, 8/10
Software Version 1.06.
This document applies to the latest software listed and higher versions. When a subsequent software version
changes the information in this document, a new issue will be released to the Beckman Coulter website. For
labeling updates, go to www.beckmancoulter.com and download the most recent manual or system help for
your instrument.
PN 4237416DB 5
REVISION STATUS
6 PN 4237416DB
CONTENTS
CONTENTS, 1-7
INTRODUCTION, 1-11
CONVENTIONS, 1-12
GRAPHICS, 1-13
SYMBOLS, 1-13
Safety Symbols, 1-13
Procedure Symbols, 1-13
PN 4237416DB 7
CONTENTS
2.5 RUNNING ACT TRON CELL CONTROL (ACT Tron Mode Only), 2-19
5 CALIBRATION, 5-1
8 PN 4237416DB
CONTENTS
PN 4237416DB 9
CONTENTS
REFERENCES, REFERENCES-1
GLOSSARY, GLOSSARY-1
ABBREVIATIONS, ABBREVIATIONS-1
INDEX, INDEX-1
TRADEMARKS, 1-11
ILLUSTRATION
6.1 Syringe Assembly Without Covers, 6-51
6.2 Syringe Assembly With Covers, 6-51
TABLES
6.1 Maintenance Schedule, 6-1
6.2 Diluter Functions Screen, 6-69
6.3 Diagnostic Functions Screen, 6-70
6.4 What Flags Mean, 6-74
6.5 Warning Messages, 6-81
6.6 Fatal Errors, 6-84
6.7 Power Problems, 6-86
6.8 Aspiration Problems, 6-86
6.9 Background Problems, 6-88
6.10 Irregular Sample Results, 6-91
6.11 ACT Tron Cell Control Results, 6-95
10 PN 4237416DB
INTRODUCTION
s Getting started
s Running your instrument day to day
s Reviewing unusual results, including how to read a result report and what flags mean
s Performing special procedures such as cleaning, replacing, or adjusting a component of
the instrument
s Troubleshooting problems with your instrument.
PN 4237416DB 11
INTRODUCTION
ABOUT THIS MANUAL
CONVENTIONS
This manual uses the following conventions:
12 PN 4237416DB
INTRODUCTION
GRAPHICS
GRAPHICS
All graphics, including screens and printouts, are for illustration purposes only and must not
be used for any other purpose.
SYMBOLS
Safety Symbols
Safety symbols alert you to potentially dangerous conditions. These symbols, together with
text, apply to specific procedures and appear as needed throughout this manual.
Biohazard.Consider all materials Wear standard laboratory attire and follow safe
! (specimens, reagents, controls, and
calibrators, and so forth) as being
laboratory procedures when handling any
material in the laboratory.
potentially infectious.
Probe hazard. The probe is sharp Avoid any unnecessary contact with the probe
! and may contain biohazardous
materials, including controls and
and probe area.
calibrators.
Electrical shock hazard. Possibility Before continuing, unplug the ACT diff
! of electrical shock when
instrument is plugged in to the
analyzer from the electrical outlet.
power source.
Procedure Symbols
Procedure symbols give direction.
INSTALLATION PROCEDURES
See the Installation and Training Guide for installation procedures.
PN 4237416DB 13
INTRODUCTION
INSTALLATION PROCEDURES
14 PN 4237416DB
INTRODUCTION
TOUCH SCREEN ICONS
Setup Startup
Sample Results
Screen
PN 4237416DB 15
INTRODUCTION
TOUCH SCREEN ICONS
Units Transmission
Date/Time Printers/Profiles
Laboratory ID Exit
QA Screen Icons
4C PLUS
Cell Control Run Calibration
4C PLUS
Management Reproducibility
Exit
16 PN 4237416DB
INTRODUCTION
TOUCH SCREEN ICONS
Dispense Lytic
Wet Prime Reagent
Voltages/Sensors Motors
PN 4237416DB 17
INTRODUCTION
TOUCH SCREEN ICONS
Delete Exit
18 PN 4237416DB
INTRODUCTION
ICON TREE OVERVIEW
DRY PRIME
LYTIC REAGENT 4C PLUS
MANAGEMENT
TRANSMISSION VERIFY
PREDILUTE
DISPENSE
LYTIC REAGENT ASSAY VALUES
AND RANGES
CALIBRATION SAMPLE
FACTORS DETAILS
ZAP
CALIBRATION
APERTURES
ASSIGNED VALUES
PRINTERS/ MOTORS
PROFILES
RINSE + MIX
REPRODUCIBILITY
RUN
PULSE
LABORATORY
ID
DRY PRIME
DILUENT CARRYOVER
RUN
LATEX
PRINT SETUP CALIBRATION
REPORT SWEEP FLOW
PREPARE TO
SHIP 7422034B
CLEAN BATHS
PN 4237416DB 19
INTRODUCTION
ICON TREE OVERVIEW
20 PN 4237416DB
INTRODUCTION
ICON TREE DETAIL
POWERUP SCREEN
CALIBRATION FACTORS
PATIENT LIMITS
(22&23) (26)
DILUTER FUNCTIONS
CLEAN BATHS SHUTDOWN
(1)
(40) (50)
VOLTAGES/SENSORS MOTORS CONTROL RUN CALIBRATION ASSIGNED VALUES
SAMPLE ID
SOLENOIDS PULSE HEIGHT CONTROL INFO REPRODUCIBILITY RUN
SAMPLE DETAILS
(46) (51) (55)
PREPARE
TO SHIP
(42) (52) (58)
CALIBRATION
RUN
(56)
LATEX CARRYOVER
CALIBRATION SUMMARY
(60)
RUN
CALIBRATION
(57) SUMMARY
Note: Screen numbers are in parentheses above the
screen for ease of reading. These numbers are
for reference when troubleshooting. Not all
screens have numbers.
7416020C
PN 4237416DB xxi
INTRODUCTION
ICON TREE DETAIL
xxii PN 4237416DB
1ROUTINE PROCEDURES 1
1.1 STARTUP
When you turn on the instrument, it automatically performs the startup procedure.
If you want to have the instrument do the startup procedure again when the instrument is on,
follow this procedure.
1.2 SHUTDOWN
Before you turn the instrument off, do this shutdown procedure.
PN 4237416DB 1-1
ROUTINE PROCEDURES
SHUTDOWN
1-2 PN 4237416DB
2CELL CONTROLS 2
2.1 ENTERING CELL CONTROL INFORMATION
Before running controls, the values from the TABLE OF EXPECTED RESULTS in the assay
sheet must be entered and saved into the instrument for each lot of controls.
When operating this instrument outside the optimal temperature range (20 - 25C), control
results may exceed the expected limits. One of the suggested corrective actions is to establish
your own mean values that are appropriate for your laboratorys environment. These values
should be entered and saved in the instrument. The mean value you establish should not
exceed the expected range limits determined for the control material at optimal temperature.
If this occurs, contact your Beckman Coulter Representative.
IMPORTANT Risk of existing data in the database not being flagged using new values or ranges. If the
Expected Values or Range is edited and saved when the control database is not empty, samples run after the
change will be flagged according to the edited values; however, the data already in the database will not be
reflagged based on the new values or ranges. The new values will be printed with the control summary data.
Be sure to edit/save Expected Values or Ranges only when the control database is empty.
! TM
!
CO
UL
TE
R
CE
LL
CO
NT
RO
L
PN 4237416DB 2-1
CELL CONTROLS
ENTERING CELL CONTROL INFORMATION
2-2 PN 4237416DB
CELL CONTROLS
ENTERING CELL CONTROL INFORMATION 2
7 8 9 0
07
74
LO
T
00
07
74
00
EX
P.
Sto
re 5
13
at 046
(3
2-8 F
OC
C )
T9
8
PN 4237416DB 2-3
CELL CONTROLS
ENTERING CELL CONTROL INFORMATION
10 Do Entering Values.
2-4 PN 4237416DB
CELL CONTROLS
ENTERING CELL CONTROL INFORMATION 2
Entering Values
IMPORTANT Risk of misleading results if improper values are entered. If you are not using 4C PLUS cell
control, DO NOT do this procedure.
If you are using ACT Tron cell control, skip this procedure.
PN 4237416DB 2-5
CELL CONTROLS
ENTERING CELL CONTROL INFORMATION
2-6 PN 4237416DB
CELL CONTROLS
ENTERING CELL CONTROL INFORMATION 2
PN 4237416DB 2-7
CELL CONTROLS
ENTERING CELL CONTROL INFORMATION
2-8 PN 4237416DB
CELL CONTROLS
RUNNING CONTROLS 2
2.2 RUNNING CONTROLS
The two controls for the ACT diff analyzer are 4C PLUS cell control and ACT Tron cell
control. ACT Tron cell control monitors the performance of the CBC parameters only and is
not available in the USA.
When operating this instrument outside the optimal temperature range (20 - 25C), control
results may exceed the expected limits. One of the suggested corrective actions is to establish
your own mean values that are appropriate for your laboratorys environment. These values
should be entered and saved in the instrument. The mean value you establish should not
exceed the expected range limits determined for the control material at optimal temperature.
If this occurs, contact your Beckman Coulter Representative.
assay sheet.
Sto
EX13O
re 50
at 46
P. C
(3
2-8 F
C )
St
T
or (35
98
e
13
at 046
2- F
OC
8 )
C
T9
8
PN 4237416DB 2-9
CELL CONTROLS
RUNNING CONTROLS
L - for low
N - for normal
H - for high
2-10 PN 4237416DB
CELL CONTROLS
RUNNING CONTROLS 2
CO
UL
RO
TE
R
CE
L
LL
CO
NT
R
OL
8 Invert the tube once or twice prior to cycling.
PN 4237416DB 2-11
CELL CONTROLS
RUNNING CONTROLS
2-12 PN 4237416DB
CELL CONTROLS
RUNNING CONTROLS 2
Printing Stored 4C PLUS Cell Control Results
r A = all
r L = low
r N = normal
r H = high
PN 4237416DB 2-13
CELL CONTROLS
DOWNLOADING 4C PLUS CELL CONTROL RESULTS FOR IQAP
Save old reagent management cards to use for this procedure. The card is old if you
see this warning on your instrument
Apply the IQAP identification label to an old reagent management card, using care not to
cover up the microchip (gold square).
2-14 PN 4237416DB
CELL CONTROLS
DOWNLOADING 4C PLUS CELL CONTROL RESULTS FOR IQAP 2
PN 4237416DB 2-15
CELL CONTROLS
DELETING 4C PLUS CELL CONTROL FILES
Once deleted, the control files cannot be recovered. Therefore, be sure that you have all the
control information you need before deleting anything.
2-16 PN 4237416DB
CELL CONTROLS
DELETING 4C PLUS CELL CONTROL FILES 2
PN 4237416DB 2-17
CELL CONTROLS
DELETING 4C PLUS CELL CONTROL FILES
r A = all
r L = low
r N = normal
r H = high
2-18 PN 4237416DB
CELL CONTROLS
RUNNING ACT TRON CELL CONTROL (ACT Tron Mode Only) 2
2.5 RUNNING ACT TRON CELL CONTROL (ACT Tron Mode Only)
This section contains an overview of the procedure. For complete instructions, see the
procedure in the ACT Tron cell control package insert.
IMPORTANT Only run ACT Tron cell control in the ACT Tron mode. Running ACT Tron in an
incorrect analyzing mode will cause wrong results.
Risk of low results. Removal of the cap from the vial before warming and mixing prevents uniform
resuspension of the cells. Do not remove the cap from the vial before warming and mixing.
Risk of high results. Aspiration of the control from the same vial more than 31 times might cause high
results. Do not aspirate more than 31 times from a single vial.
PN 4237416DB 2-19
CELL CONTROLS
RUNNING ACT TRON CELL CONTROL (ACT Tron Mode Only)
2-20 PN 4237416DB
CELL CONTROLS
RUNNING ACT TRON CELL CONTROL (ACT Tron Mode Only) 2
PN 4237416DB 2-21
CELL CONTROLS
RUNNING ACT TRON CELL CONTROL (ACT Tron Mode Only)
2-22 PN 4237416DB
N
3RUNNING SAMPLES 3
3.1 GENERAL
If it is your laboratorys procedure to collect specimens for hematology via capillary collection
into a microcollection device, you may run the specimen in the Whole Blood mode. However,
the Predilute mode should be used if the specimen collected cannot be directly aspirated in a
whole blood mode.
When you have set the ACT diff analyzer to the correct analyzing mode (Whole Blood or
Predilute) and have verified the sample ID, you are ready to run samples.
To ensure that the blood specimen is analyzed correctly, you must set the instrument to the
correct analyzing mode (Whole Blood or Predilute). Note: ACT Tron mode is used only for
ACT Tron cell control, not for patient samples.
IMPORTANT Risk of misleading results. Running a blood sample in an incorrect analyzing mode can cause
wrong results. Only run a whole blood sample in the Whole Blood mode.
Beckman Coulter suggests that:
PN 4237416DB 3-1
RUNNING SAMPLES
RUNNING WHOLE BLOOD SAMPLES
4 5 6
autoincremented.
Note: If autosequencing is off, the probe
does not descend until you manually enter
and save the next ID.
3-2 PN 4237416DB
RUNNING SAMPLES
RUNNING WHOLE BLOOD SAMPLES 3
! 8x
PN 4237416DB 3-3
RUNNING SAMPLES
RUNNING WHOLE BLOOD SAMPLES
3-4 PN 4237416DB
RUNNING SAMPLES
RUNNING PREDILUTED BLOOD SAMPLES 3
3.3 RUNNING PREDILUTED BLOOD SAMPLES
If it is your laboratorys procedure to collect specimens for hematology via capillary collection
into a microcollection device, you may run the specimen in the Whole Blood mode. However,
the Predilute mode should be used if the specimen collected cannot be directly aspirated in a
whole blood mode. Predilute mode dispenses 1580 L of diluent into an empty tube or
receptacle where 20 L of capillary blood will be added, thereby diluting it, to create an
adequate amount of sample volume for the instrument to aspirate for analysis.
IMPORTANT Risk of misleading results. Running a blood sample in an incorrect analyzing mode can cause
wrong results. Only run prediluted blood in the Predilute mode.
Beckman Coulter suggests that:
PN 4237416DB 3-5
RUNNING SAMPLES
RUNNING PREDILUTED BLOOD SAMPLES
autoincremented. 7 8 9 0
3-6 PN 4237416DB
RUNNING SAMPLES
RUNNING PREDILUTED BLOOD SAMPLES 3
laboratorys protocol.
1580 L
c. Wait at least 5 minutes before
running the sample.
PN 4237416DB 3-7
RUNNING SAMPLES
RUNNING PREDILUTED BLOOD SAMPLES
3-8 PN 4237416DB
4REVIEWING RESULTS 4
4.1 PRINTING STORED SAMPLE RESULTS FOR VIEWING
As mentioned in Chapter 3, RUNNING SAMPLES, the instrument automatically saves (or
stores) the patient results once the sample is analyzed. There may be times when you need to
review certain patient results that were saved. This procedure explains how to do that.
This function is available for use with the graphic printer only.
Note: Results print based on the patient range selected when the sample was run.
PN 4237416DB 4-1
REVIEWING RESULTS
PRINTING STORED SAMPLE RESULTS FOR VIEWING
1 2 3
b. Enter the ending date of the sample 5
4 6 -
results you want. 7 8 9 0
4-2 PN 4237416DB
5CALIBRATION 5
5.1 OVERVIEW
Beckman Coulter calibrates the ACT diff analyzer at the factory before shipment. You may
need to perform calibration procedures when you replace any instrument component that
involves the primary measurement characteristics (such as an aperture).
Because the instrument is electronically stable, it should not require frequent recalibration
when you operate it and maintain it according to the recommendations in this manual. Make
the decision to recalibrate based on the performance of your quality control program.
Beckman Coulter recommends that you calibrate your instrument according to the
regulations required by your inspecting agency.
Your laboratorys quality control program should continually monitor and confirm
instrument calibration. Review your control results periodically. Keep a written record of this
review. To confirm calibration of the ACT diff analyzer:
1. Verify that 95% of control results are within their ranges as listed in the TABLE OF
EXPECTED RESULTS.
2. Verify that there are no unexplained shifts or trends in the data.
If recalibration appears necessary, but you have not replaced a component affecting
calibration, do NOT recalibrate the instrument.
1. First, thoroughly clean your analyzer following the Clean the Baths procedure in
Chapter 6 of this manual.
2. Then reanalyze a new vial of control material.
r If the control results are still outside of the expected ranges, refer to Table 6.10 and
Heading 6.21 in this manual.
r If the results remain outside the expected ranges, call your Beckman Coulter
Representative before recalibrating.
When necessary, perform calibration by following the procedures given in this section.
Before you begin calibration, be sure you have enough reagents to perform the complete
procedure. If you run out of reagents during calibration, you must start over and perform a
complete calibration.
Recommended Calibrator
Beckman Coulter recommends using S-CAL calibrator for automated calibration. If S-CAL
calibrator is not available, manual calibration can be done after Reproducibility and
Carryover are completed. See Appendix A for the manual calibration procedure.
PN 4237416DB 5-1
CALIBRATION
BEFORE CALIBRATING
1. Do precalibration checks.
2. Do Reproducibility.
3. Do Carryover.
Precalibration Checks
5-2 PN 4237416DB
CALIBRATION
REPRODUCIBILITY 5
5.3 REPRODUCIBILITY
The ACT diff analyzer includes a Reproducibility function that automatically performs
calculations on the samples you run.
r Whole Blood
r Predilute
r QC Check
It is recommended that you run Reproducibility in the Whole Blood mode using either whole
blood or a cell control with a known range of values, such as 4C PLUS cell control.
If it is your laboratorys procedure to collect specimens for hematology via capillary collection
into a microcollection device, you may run the specimen in the Whole Blood mode. However,
the Predilute mode should be used if the specimen collected cannot be directly aspirated in a
whole blood mode.
To perform statistics, the instrument requires three acceptable samples. If a result is not
acceptable, the instrument automatically rejects the result. You can also reject a result by
touching the Reject icon.
There is a summary screen for Reproducibility. Autoprint, if turned ON, prints a sample
report upon completion of each run. Autoprint is not an option for printing the summary
report; you must manually select the print summary icon. You can print a summary report
beginning with the third sample all the way to the thirty-first sample, if you choose to run
that many samples.
When doing Reproducibility, Carryover, or Calibration, do not leave the screen until you
finish analyzing the required number of samples. Leaving the screen without finishing the
required analysis will delete your data, which means that you must restart the test.
PN 4237416DB 5-3
CALIBRATION
REPRODUCIBILITY
5-4 PN 4237416DB
CALIBRATION
REPRODUCIBILITY 5
PN 4237416DB 5-5
CALIBRATION
REPRODUCIBILITY
7420110B
5-6 PN 4237416DB
CALIBRATION
CARRYOVER 5
5.4 CARRYOVER
Carryover is a check to make sure that no part of a sample is carried over to the next sample,
thus affecting the next samples results. Carryover:
Note: You may use 4C PLUS cell control as an alternative to normal whole-blood samples.
When doing Reproducibility, Carryover, or Calibration, do not leave the screen until you
finish analyzing the required number of samples. Leaving the screen without finishing the
required analysis will delete your data, which means that you must restart the test.
PN 4237416DB 5-7
CALIBRATION
CARRYOVER
5-8 PN 4237416DB
CALIBRATION
CARRYOVER 5
PN 4237416DB 5-9
CALIBRATION
AUTO-CALIBRATION
5.5 AUTO-CALIBRATION
The S-CAL calibration kit helps you determine whether the calibration factors of the
instrument need to be changed. Assigned values are provided in the S-CAL calibration kit
package insert. Only the package inserts provided with the S-CAL calibration kit provide
the correct assigned values for the calibrator.
For automated calibration, you simply cycle the S-CAL calibrator. After you enter the
ASSIGNED VALUES from the S-CAL calibrator package insert, calculations and comparisons
to assigned values are done automatically by the instrument. You can save the calibration
data.
After calibration is completed, a PASSED, NEEDED or FAILED message appears for each
parameter.
When doing Reproducibility, Carryover, or Calibration, do not leave the screen until you
finish analyzing the required number of samples. Leaving the screen without finishing the
required analysis will delete your data, which means that you must restart the test.
5-10 PN 4237416DB
CALIBRATION
AUTO-CALIBRATION 5
PN 4237416DB 5-11
CALIBRATION
AUTO-CALIBRATION
keypad.
5-12 PN 4237416DB
CALIBRATION
AUTO-CALIBRATION 5
PN 4237416DB 5-13
CALIBRATION
AUTO-CALIBRATION
5-14 PN 4237416DB
CALIBRATION
AUTO-CALIBRATION 5
7420111A
PN 4237416DB 5-15
CALIBRATION
AUTO-CALIBRATION
5-16 PN 4237416DB
6 SERVICE AND MAINTENANCE 6
6.1 GENERAL MAINTENANCE
This chapter details the ACT diff analyzer maintenance procedures that are your
responsibility. It also includes a troubleshooting guide to help you solve instrument problems.
CAUTION Incorrectly performed maintenance procedures can damage the ACT diff analyzer. Do not attempt
any procedures that are not included in this manual or in the instrument replacement cards. Call your
Beckman Coulter Representative for service and maintenance beyond the scope of Beckman Coulter
documentation.
Cycle Counter
The cycle counter appears on the Startup results screen and on the Diagnostics screen. The
cycle number prints on the Startup report.
PN 4237416DB 6-1
SERVICE AND MAINTENANCE
MAINTENANCE SCHEDULE
Replace fuses When blown r No power. Green power LED is not lit.
r Instrument is plugged in but does not run.
Replace diluent filters When necessary r When you get excessive diluent empty messages.
r When you replace peristaltic pump tubing.
r When a filter is clogged.
Replace peristaltic pump When necessary r When you get excessive diluent empty messages.
tubing r When you replace diluent filters.
r Tubing is worn to the extent that it looks almost worn
through.
12,000
Replace probe wipe block When defective or Fluid drips from probe wipe but vacuum is good and instrument
plugged. works.
Replace tubing Every 3 years When cracked, leaking or has lost resilience.
6-2 PN 4237416DB
SERVICE AND MAINTENANCE
MAINTENANCE SCHEDULE 6
Table 6.1 Maintenance Schedule (Continued)
Replace reagents When empty When instrument reports empty and the container is empty.
PN 4237416DB 6-3
SERVICE AND MAINTENANCE
MAINTENANCE SCHEDULE
6-4 PN 4237416DB
SERVICE AND MAINTENANCE
CLEANING PROCEDURES 6
6.3 CLEANING PROCEDURES
These are not routine procedures. Use them only if necessary for troubleshooting or before
calibrating.
Zap Aperture
Zap the aperture when the instrument:
PN 4237416DB 6-5
SERVICE AND MAINTENANCE
CLEANING PROCEDURES
6-6 PN 4237416DB
SERVICE AND MAINTENANCE
CLEANING PROCEDURES 6
PN 4237416DB 6-7
SERVICE AND MAINTENANCE
CLEANING PROCEDURES
Clean the inside of the instrument (behind the front door and beneath the bath shield) with a
damp cloth and distilled water if obvious evidence of corrosive deposits exists. Be careful not
to wipe contaminants into the bath. Utilize appropriate barrier protection, as these areas may
contain biohazardous material.
6-8 PN 4237416DB
SERVICE AND MAINTENANCE
CALIBRATION PROCEDURES 6
6.4 CALIBRATION PROCEDURES
See Chapter 5, Calibration.
b c
1@ VAC ADJ
1!
RBC WBC
1)
LV16 LV17
d
j
i h g f e
B Software Card Slot H RBC Bath
C Vacuum Adjust I Aspirate Switch
D Vacuum Isolator Chamber J Probe
E Hgb Lamp 1) Probe Wipe Block
F Sweepflow Spool 1! Horizontal Traverse Assembly
G WBC Bath 1@ Horizontal Traverse Motor
PN 4237416DB 6-9
SERVICE AND MAINTENANCE
ACT diff ANALYZER COMPONENT LOCATIONS
Inside Right
b1
c2
h7 g
6 f
5 e
4 d
3
6-10 PN 4237416DB
SERVICE AND MAINTENANCE
ACT diff ANALYZER COMPONENT LOCATIONS 6
Valves, Inside Front
VAC ADJ
RBC WBC
LV16 LV17
LV16 LV17
LV16 On = Opens count path from RBC LV17 ON = Opens count path from WBC bath.
bath. OFF = Closes count path from WBC bath.
OFF = Closes count path from RBC
bath.
PN 4237416DB 6-11
SERVICE AND MAINTENANCE
ACT diff ANALYZER COMPONENT LOCATIONS
LV10
LV1
LV9 LV2
LV3
LV4
LV5
LV13
LV14
LV15
LV1 Open top of Vacuum Isolator Chamber to LV9 ON = Lytic reagent syringe connected to bath.
vent. OFF = Lytic reagent syringe connected to lytic
reagent source.
LV2 ON = High vacuum. LV10 ON = Diluent pump goes to the probe wash.
OFF = Low vacuum. OFF = Diluent pump goes to the diluent reservoir.
LV3 ON = Sends mixing bubbles to the WBC LV11 ON = Diluent from the syringe goes to LV7 (bath
bath. prefill).
OFF = Sends mixing bubbles to the RBC OFF = Probe connected to syringes for aspirate or
bath. diluent dispense.
LV4 ON = side of WBC bath. LV12 ON = Diluent syringe connected to aspirate
OFF = bottom of WBC bath. syringe.
LV5 ON = Vacuum pump vent sending mixing OFF = Diluent syringe connected to diluent
bubbles. reservoir.
OFF = Vacuum pump venting to LV13 ON = Waste pump inputs from cleaner.
atmosphere. OFF = Waste pump outputs to waste.
LV7 ON = Prefill to RBC bath. LV14 ON = Drains WBC bath.
OFF = Prefill to WBC bath. OFF = Drains RBC bath.
LV8 ON = Opens probe wash drain to vacuum LV15 ON = Drains vacuum isolator chamber.
isolator chamber. OFF = Drains bath specified by LV14.
OFF = closes probe wash drain to vacuum
isolator chamber.
6-12 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING REAGENTS 6
6.6 REPLACING REAGENTS
For information on connecting the reagents the first time, see the Installation and Training
Guide.
Change the reagent container when you see one of these symbols:
PN 4237416DB 6-13
SERVICE AND MAINTENANCE
REPLACING REAGENTS
6-14 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING REAGENTS 6
PN 4237416DB 6-15
SERVICE AND MAINTENANCE
REPLACING REAGENTS
6-16 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING REAGENTS 6
PN 4237416DB 6-17
SERVICE AND MAINTENANCE
REPLACING REAGENTS
6-18 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING REAGENTS 6
instrument.
PN 4237416DB 6-19
SERVICE AND MAINTENANCE
REPLACING REAGENTS
6-20 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING REAGENTS 6
PN 4237416DB 6-21
SERVICE AND MAINTENANCE
REPLACING REAGENTS
6-22 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING REAGENTS 6
Replacing the AC T Rinse Shutdown Diluent
Replace the ACT Rinse shutdown diluent container when you see:
PN 4237416DB 6-23
SERVICE AND MAINTENANCE
REPLACING REAGENTS
6-24 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING THE WASTE CONTAINER 6
6.7 REPLACING THE WASTE CONTAINER
Replace the waste container when you see:
WARNING Waste can include biohazardous material that could cause contamination. Handle and dispose of
according to acceptable laboratory standards.
Do not operate the instrument if the waste sensor level is disconnected.
PN 4237416DB 6-25
SERVICE AND MAINTENANCE
REPLACING DILUENT FILTERS
Note: If the vacuum fluid barrier filter becomes plugged, replace it with the following
method.
WARNING Possible injury to hands. The peristaltic pumps rotate at various intervals during a normal run. To
avoid injury, do not put your hands in the area while the instrument is on.
6-26 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING DILUENT FILTERS 6
PN 4237416DB 6-27
SERVICE AND MAINTENANCE
REPLACING DILUENT FILTERS
6-28 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING DILUENT FILTERS 6
PN 4237416DB 6-29
SERVICE AND MAINTENANCE
REPLACING PERISTALTIC PUMP TUBING
WARNING Possible injury to hands. The peristaltic pumps rotate at various intervals during a normal run. To
avoid injury, do not put your hands in the area while the instrument is cycling
6-30 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING PERISTALTIC PUMP TUBING 6
WARNING The waste pump tubing can contain biohazardous material that can cause contamination if not handled
properly. Handle these components according to acceptable laboratory practices.
PN 4237416DB 6-31
SERVICE AND MAINTENANCE
REPLACING PERISTALTIC PUMP TUBING
6-32 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING PERISTALTIC PUMP TUBING 6
PN 4237416DB 6-33
SERVICE AND MAINTENANCE
REPLACING PERISTALTIC PUMP TUBING
4X
6-34 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING PERISTALTIC PUMP TUBING 6
PN 4237416DB 6-35
SERVICE AND MAINTENANCE
REPLACING CHECK VALVES
r It is clogged.
r It lets liquid or air flow both ways.
6-36 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING CHECK VALVES 6
!
WARNING Biohazardous material might be contained in the check valves and associated tubing and could cause
contamination unless handled with care. Wear protective gear. Avoid skin contact. Clean up spills immediately. Dispose of
valve and tubing according to acceptable laboratory procedures for biohazardous materials.
PN 4237416DB 6-37
SERVICE AND MAINTENANCE
REPLACING CHECK VALVES
IMPORTANT To avoid obtaining misleading results, make sure the new valve is in the same position as the old one.
6-38 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING CHECK VALVES 6
PN 4237416DB 6-39
SERVICE AND MAINTENANCE
REPLACING TUBING
6-40 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING TUBING 6
PN 4237416DB 6-41
SERVICE AND MAINTENANCE
REPLACING FUSES
1.5 Amp
Slo Blo
250V
J1
AC INPUT
F1 F2
ON ELECTRICAL POWER
SUPPLY AC INPUTS
OFF 90 - 132v / 198 - 264v
FUSE 3AG
1 . 5A 250V
WARNING
!
TO AVOID ELECTRIC CONNECT ONLY
SHOCK DISCONNECT TO A PROPERLY
POWER CORD PRIOR TO EARTH GROUNDED
REMOVING OR REPLACING OUTLET .
FUSE .
REPLACE FUSE ONLY
WITH THE TYPE AND
RATING SPECIFIED .
CAUTION Risk of electrical shock. Avoid electrical shock by unplugging the instruments power cord.
6-42 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING FUSES 6
PN 4237416DB 6-43
SERVICE AND MAINTENANCE
REPLACING VACUUM ISOLATOR CHAMBER
Needle-nose pliers are needed for this procedure; they are not provided with the instrument.
6-44 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING VACUUM ISOLATOR CHAMBER 6
!
WARNING The waste pump tubing can contain biohazardous material that could cause contamination if not handled
properly. Handle these components according to acceptable laboratory practices.
PN 4237416DB 6-45
SERVICE AND MAINTENANCE
REPLACING VACUUM ISOLATOR CHAMBER
6-46 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING VACUUM ISOLATOR CHAMBER 6
7
Note: The tubing and the VIC fittings are not
8
numbered the same due to the quantity of
fittings throughout the entire instrument.
PN 4237416DB 6-47
SERVICE AND MAINTENANCE
REPLACING VACUUM ISOLATOR CHAMBER
10
6-48 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING VACUUM ISOLATOR CHAMBER 6
PN 4237416DB 6-49
SERVICE AND MAINTENANCE
REPLACING VACUUM ISOLATOR CHAMBER
6-50 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING SYRINGE PISTONS AND SYRINGE ASSEMBLIES 6
6.14 REPLACING SYRINGE PISTONS AND SYRINGE ASSEMBLIES
To optimize instrument performance, replace the syringe pistons or syringe assemblies every
12,000 cycles. (See Heading 6.1 for instructions on how to determine the cycle count.) When
replacing more than one syringe piston, be sure to replace them one at-a-time to ensure that
you do not misplace the plungers. (See Heading 6.1 for how to determine the cycle count.)
Also, periodically check to see if excessive amounts of liquid appear to be leaking from any of
the syringes. If there are, clean the syringe and perform a reproducibility test (Heading 5.3).
Note: It is normal for a small amount of fluid to escape between the seal and the glass barrel.
The fluid is a lubricant that helps extend the life of the syringe.
There are two styles of diluent and sample syringes the original sytle (without covers) and
the modified style (equipped with a flexible, protective covers.
Figure 6.1 Syringe Assembly Without Covers Figure 6.2 Syringe Assembly With Covers
12,000
1mL
5mL
250L
PN 4237416DB 6-51
SERVICE AND MAINTENANCE
REPLACING SYRINGE PISTONS AND SYRINGE ASSEMBLIES
Note: This procedure illustrates the modified style of diluent and sample syringes. Your
instrument may be different from what is shown here.
6-52 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING SYRINGE PISTONS AND SYRINGE ASSEMBLIES 6
PN 4237416DB 6-53
SERVICE AND MAINTENANCE
REPLACING SYRINGE PISTONS AND SYRINGE ASSEMBLIES
250 L
6-54 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING SYRINGE PISTONS AND SYRINGE ASSEMBLIES 6
PN 4237416DB 6-55
SERVICE AND MAINTENANCE
REPLACING SYRINGE PISTONS AND SYRINGE ASSEMBLIES
1 mL 5 mL 250 L
6-56 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING SYRINGE PISTONS AND SYRINGE ASSEMBLIES 6
PN 4237416DB 6-57
SERVICE AND MAINTENANCE
REPLACING SYRINGE PISTONS AND SYRINGE ASSEMBLIES
6-58 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING THE PROBE WIPE 6
6.15 REPLACING THE PROBE WIPE
Replace the probe wipe when it is defective or plugged. If fluid drips from the probe wipe but
vacuum is good and the instrument works, then the probe wipe is probably defective and you
should replace it.
PN 4237416DB 6-59
SERVICE AND MAINTENANCE
REPLACING THE PROBE WIPE
!
WARNING To avoid being exposed to biohazardous material, adhere to standard laboratory safety procedures.
6-60 PN 4237416DB
SERVICE AND MAINTENANCE
REPLACING THE PROBE WIPE 6
PN 4237416DB 6-61
SERVICE AND MAINTENANCE
REPLACING THE PROBE WIPE
6-62 PN 4237416DB
SERVICE AND MAINTENANCE
PREPARING TO SHIP THE INSTRUMENT 6
6.16 PREPARING TO SHIP THE INSTRUMENT
When you have done all the troubleshooting and still cannot fix the problem, call your
Beckman Coulter Representative. If directed to, follow the authorization procedures and
prepare the instrument for shipment as follows.
Two containers, bleach, distilled water, and paper towels are needed for this procedure.
I H2
A
!
WARNING Instrument tubing can contain biohazardous material that can cause contamination if not handled properly.
Handle these components according to acceptable laboratory practices.
PN 4237416DB 6-63
SERVICE AND MAINTENANCE
PREPARING TO SHIP THE INSTRUMENT
6-64 PN 4237416DB
SERVICE AND MAINTENANCE
PREPARING TO SHIP THE INSTRUMENT 6
I H2
50%
DISTILLED
WATER
50%
BLEACH
PN 4237416DB 6-65
SERVICE AND MAINTENANCE
PREPARING TO SHIP THE INSTRUMENT
6-66 PN 4237416DB
SERVICE AND MAINTENANCE
PREPARING TO SHIP THE INSTRUMENT 6
PN 4237416DB 6-67
SERVICE AND MAINTENANCE
PREPARING TO SHIP THE INSTRUMENT
6-68 PN 4237416DB
SERVICE AND MAINTENANCE
TROUBLESHOOTING 6
6.17 TROUBLESHOOTING
Troubleshooting Tools
Knowing what your ACT diff analyzer does, how it sounds when operating properly, and
what normal results look like are the keys to troubleshooting problems. Study the Normal
Sample Flow. Then watch and listen while the instrument goes through its cycles.
If you later find that your ACT diff analyzer is not operating properly, you can begin to
isolate the problem by studying irregular results (Table 6.10) and watching the instrument
cycle a sample.
Diluter Functions
The Diluter Functions screen provides you with basic diluter functions to use in
troubleshooting. Table 6.2 describes the diluter functions
.
PN 4237416DB 6-69
SERVICE AND MAINTENANCE
TROUBLESHOOTING
Dispenses lytic reagent into the WBC Manually primes the lytic reagent system.
bath. Checks for bubbles in the lytic reagent system.
Verifies the operation of the lytic reagent pump.
Exits from the Diluter Functions screen. Returns to previous screen.
Diagnostic Functions
The Diagnostic Functions screen provides you with basic diagnostic functions to use in
troubleshooting. Table 6.3 describes the diagnostic functions.
6-70 PN 4237416DB
SERVICE AND MAINTENANCE
TROUBLESHOOTING 6
Table 6.3 Diagnostic Functions Screen
Displays motors screen CAUTION Indiscriminate use of these functions can damage the
and allows you to instrument. Do not use the motors function without instruction from
interactively run each your Beckman Coulter Representative.
motor through its normal
range of motion.
Displays an in-progress Lets you verify the electronic stability of the instrument for WBC and
screen and performs an RBC apertures.
electronics pulse test.
Latex gain Lets you set particle sizes for WBC, RBC, and Plt gains.
Calibration
Prepares the instrument Lets you drain and disinfect the instrument in preparation for shipping.
for shipping.
Displays the cycle count. Lets you view the cycle count.
PN 4237416DB 6-71
SERVICE AND MAINTENANCE
TROUBLESHOOTING
6-72 PN 4237416DB
SERVICE AND MAINTENANCE
PARAMETER CODES AND FLAGS 6
6.18 PARAMETER CODES AND FLAGS
Analyze samples within 24 hours of collection.
Report results immediately if there are no flags and if the results are within your referenced
ranges. Table 6.4 shows the parameter codes and flags that can appear with results. Also note:
r If any flag appears, review the results according to your laboratorys protocol.
r The cells of whole blood collected in EDTA undergo a process of equilibration. White
blood cells of certain samples might take up to 30 minutes to reach this state of
equilibration in the EDTA. Also, after 5 hours, cellular deterioration might start to occur.
These samples might show increased flagging of differential parameters (1, 2, 3, M). If a
sample that produces differential flags is less than 30 minutes old, reanalysis of the
sample at a later time might eliminate the differential flags.
Whole blood samples processed between 30 minutes and 5 hours of collection provide the
best differential performance for all sample types, collection devices, and collection variables.
Prediluted samples processed between 5 minutes and 1 hour of collection provide the best
differential performance for all sample types, collection devices, and collection variables.
Hierarchy of Flags
There are two types of flags:
r Those that replace the parameter results, also known as codes, and
r Those that appear next to the parameter results. Up to two of these flags can be
displayed for a parameter.
XXXXX
.....
Non-Replacement Flags
For those flags that appear next to the parameter results, the hierarchy, in decreasing order of
importance, is:
X
+
*
1, 2, 3, 4, M (where M means multiple regions)
H or L
PN 4237416DB 6-73
SERVICE AND MAINTENANCE
WHAT FLAGS AND CODES MEAN
6-74 PN 4237416DB
SERVICE AND MAINTENANCE
WHAT FLAGS AND CODES MEAN 6
Table 6.4 What Flags Mean (Continued)
----- d.
continued
e.
PN 4237416DB 6-75
SERVICE AND MAINTENANCE
WHAT FLAGS AND CODES MEAN
+++++ If for Plt, also . . . . . for MPV (Pct and PDW), For WBC, RBC, Hgb or Plt:
(pluses) and * for WBC, LY#, MO#, GR#, Hgb, MCH, MCHC. 1. Ensure that the bath shield is in place.
2. Make a dilution to determine the parameter
If for Hgb, also . . . . . for MCH and MCHC. result:
a. Dilute 1 part thoroughly mixed sample
If for RBC, * for MCV, RDW, Plt, MPV (Pct and with 1 part normal saline (0.85% NaCl)
PDW) and Hct; also . . . . . for MCH and MCHC. in a clean test tube.
b. Mix then immediately run the dilution
in whole blood mode.
If for WBC, also . . . . . for LY%, MO%, GR%, c. Multiply the parameter result
LY#, MO#, GR#; also * for RBC, Hgb, Hct, MCV, by 2.
MCH, MCHC, RDW, Plt, and MPV (Pct and PDW). Corrected result = (Dilution result x 2)
d. Correct derived parameters if
applicable.
e. If result still gives +++++, increase
dilution and repeat.
6-76 PN 4237416DB
SERVICE AND MAINTENANCE
WHAT FLAGS AND CODES MEAN 6
Table 6.4 What Flags Mean (Continued)
b.
c.
d.
e.
PN 4237416DB 6-77
SERVICE AND MAINTENANCE
WHAT FLAGS AND CODES MEAN
6-78 PN 4237416DB
SERVICE AND MAINTENANCE
WHAT FLAGS AND CODES MEAN 6
Table 6.4 What Flags Mean (Continued)
WBC >99.9 <150 x 103/uL If any parameter is outside linearity limits, cycle
diluent blank before proceeding with subsequent
RBC >7.00 <8.00 x106/uL
samples.
Hgb >25.0 <30.0 g/dL
Plt >999 < 3000 x 103/uL
If for WBC, also * for RBC, Hgb, MCV, Hct, MCH,
MCHC, RDW, Plt, MPV (Pct and PDW), and
. . . . . for LY#, MO#, GR#, LY%, MO%, and
GR%.
If for RBC, also * for Hct, MCH MCHC, MCV, RDW,
Plt, MPV, (Pct and PDW).
If for Plt, also * for MPV (Pct and PDW), WBC,
LY#, MO#, GR#, Hgb, MCH, MCHC.
If for Hgb, also * for MCH and MCHC.
1, 2, 3, 4, M Differential parameters failed the internal regional Verify results according to your laboratorys
size distributional criteria at one specific region protocol.
(1, 2, 3 or 4) or multiple regions (M).
RELATIVE NUMBER
LY MO GR
1 2 3 4
30 fL 35 fL 90 fL 160 fL 450 fL
SIZE IN fL
PN 4237416DB 6-79
SERVICE AND MAINTENANCE
WHAT FLAGS AND CODES MEAN
6-80 PN 4237416DB
SERVICE AND MAINTENANCE
WHAT WARNING MESSAGES MEAN 6
6.20 WHAT WARNING MESSAGES MEAN
Table 6.5 describes the warning messages and suggested recovery action.
PN 4237416DB 6-81
SERVICE AND MAINTENANCE
WHAT WARNING MESSAGES MEAN
Control file full There is no more storage space available for your 4C PLUS control files. If
your laboratory is an IQAP participant, save the control data to a depleted
reagent management card. See Downloading 4C PLUS Cell Control Results
for IQAP in Chapter 5. To make room for additional control file storage, you
may want to delete some existing control files. See Deleting 4C PLUS Cell
Control Results in Chapter 5.
Control expired Do not use this control; it has expired. Use a control that is not expired.
Patient data or If this appears during startup, print what is currently stored to determine
control data corrupted what patient data, if any, are present. Depending on the type of error, one or
possibly all patient samples previously stored may be gone.
Check ACT diff Reagent Make sure card is in reader correctly. If problem persists, it may be time for
Management card new reagent with a new card. If problem occurs with a new card, there
could be a problem with the card, card reader, card reader connection, or
card reader controller (which is part of the display assembly).
Diluent empty r If reagent container does not appear empty, try priming first.
r If there appears to be reagent, but it does not fill properly, check for
proper position of pickup tube, air leaks in tubing from cube to
reservoir, worn peristaltic pump tubing, or a partially plugged blue
filter to the diluent peristaltic pump PM2.
r Also check for crimps or plugs in the tubing from the reagent pickup
through the peristaltic tubing and filter, to the bottom of the reservoir.
These could affect reservoir fill.
r If reservoir is overfilled, replace peristaltic pump tubing (Heading 6.9)
and diluent filters (Heading 6.8).
6-82 PN 4237416DB
SERVICE AND MAINTENANCE
WHAT WARNING MESSAGES MEAN 6
Table 6.5 Warning Messages (Continued)
PN 4237416DB 6-83
SERVICE AND MAINTENANCE
FATAL ERRORS
Turn OFF the instrument, then turn it ON to see if the error is corrected. Table 6.6 offers
some suggested actions. If these do not solve the problem, call your Beckman Coulter
Representative.
6-84 PN 4237416DB
SERVICE AND MAINTENANCE
FATAL ERRORS 6
Table 6.6 Fatal Errors (Continued)
Ensure that there are no plugs near the vacuum source, such as a plug in the fluid
barrier (green striped).
PN 4237416DB 6-85
SERVICE AND MAINTENANCE
TROUBLESHOOTING GUIDES
No voltage or wrong voltage at Make sure voltage is on and outlet is 90-264 Vac. Check
laboratory power outlet. fuses, replace if necessary.
2. Problem with connection 2. Check to see that the connector on top of the 250 L
to syringe module. syringe module is tight and there is no air in the tubing
or syringe. See Heading 6.13.
3. Problem with LV11. This 3. The small brown knob on top of the solenoid valves
valve is in the aspiration will move when the valve energizes. Check that this
path and a plug or moves during a cycle.
incorrect position would
stop aspiration.
Incomplete aspiration It is very difficult to tell an Check for the same problems as above. They will be partial
incomplete aspiration when you leaks or plugs instead of pulled-off tubes or total plugs.
are aspirating only 12 L. This Also, LV12 may steal some of the aspirate volume if it
conclusion can only be arrived at does not completely block its connection to the syringe
by analyzing the results. WBC, during aspiration.
RBC, Hgb and Plt would have to
be low, with MCV normal.
6-86 PN 4237416DB
SERVICE AND MAINTENANCE
TROUBLESHOOTING GUIDES 6
Table 6.8 Aspiration Problems (Continued)
2. Fluid drips outside the 2. The probe wipe is not working. Check for leaks in the
probe. tubing to the probe wipe, a plug in the lower waste
port of the probe wipe, a plug in the tubing between
the lower probe wipe port and the Vacuum Isolator
Chamber, a vacuum leak at the Vacuum Isolator
Chamber, or no vacuum. Check to ensure that the
vacuum pump is turned on and is working. (Vacuum
pump is under left side door.)
Bubbles in aspirator 1. Leak from syringe to 1. If air is in these lines, check the components and
tubing between tip and aspirate tip. tubing for partial or no aspiration.
aspirator pump.
2. Leak between diluent 2. A leak from the reservoir to the syringe assembly will
reservoir and syringe cause air to be in the aspirate and diluent syringe and
assembly. in the line to the aspirate probe. This would involve the
tubing, LV11 and LV12. Also check for leaks at the
diluent or aspirate syringe.
PN 4237416DB 6-87
SERVICE AND MAINTENANCE
TROUBLESHOOTING GUIDES
6-88 PN 4237416DB
SERVICE AND MAINTENANCE
TROUBLESHOOTING GUIDES 6
Table 6.9 Background Problems (Continued)
PN 4237416DB 6-89
SERVICE AND MAINTENANCE
TROUBLESHOOTING GUIDES
6-90 PN 4237416DB
SERVICE AND MAINTENANCE
TROUBLESHOOTING GUIDES 6
Table 6.10 Irregular Sample Results
PN 4237416DB 6-91
SERVICE AND MAINTENANCE
TROUBLESHOOTING GUIDES
6-92 PN 4237416DB
SERVICE AND MAINTENANCE
TROUBLESHOOTING GUIDES 6
Table 6.10 Irregular Sample Results (Continued)
PN 4237416DB 6-93
SERVICE AND MAINTENANCE
TROUBLESHOOTING GUIDES
6-94 PN 4237416DB
SERVICE AND MAINTENANCE
TROUBLESHOOTING GUIDES 6
Table 6.11 ACT Tron Cell Control Results
PN 4237416DB 6-95
SERVICE AND MAINTENANCE
TROUBLESHOOTING GUIDES
6-96 PN 4237416DB
AMANUAL CALIBRATION A
A.1 ANALYSIS PROCEDURE
Use a material with known reference values as your calibrator.
PN 4237416DB A-1
MANUAL CALIBRATION
ANALYSIS PROCEDURE
4
5
6
7
8
9
10
11
TOTAL
MEAN (A)
ASSIGNED VALUE (B)
ABSOLUTE DIFFERENCE (C)
CALIBRATION REQUIRED
CURRENT CALIBRATION FACTOR (D)
NEW CALIBRATION FACTOR (E)
C=B-A
E = (B / A) x D
A-2 PN 4237416DB
MANUAL CALIBRATION
CALCULATIONS PROCEDURE A
A.2 CALCULATIONS PROCEDURE
PN 4237416DB A-3
MANUAL CALIBRATION
CALCULATING NEW CALIBRATION FACTORS
A-4 PN 4237416DB
MANUAL CALIBRATION
CALCULATING NEW CALIBRATION FACTORS A
PN 4237416DB A-5
MANUAL CALIBRATION
CALCULATING NEW CALIBRATION FACTORS
Calibration Worksheet
10
11
TOTAL
MEAN (A)
CALIBRATION REQUIRED
A = samples 2 through 11
C=B-A
E = (B / A) x D
A-6 PN 4237416DB
REFERENCES
1. Coulter WH. High speed automatic blood cell counter and cell size analyzer. Paper
presented at National Electronics Conference, Chicago, IL, 1956; October 3.
2. Brecher GM, Schneiderman M and Williams GZ. Evaluation of electronic red blood cell
counter. Am J Clin Path, 1956; 26:1439-1449.
3. Brittin GM, Brecher G and Johnson CA. Evaluation of the COULTER COUNTER Model
S. Am J Clin Path, 1969; 52:780-783
4. Gottmann NAW. Multiple hematologic analyses by means of a COULTER COUNTER
Model S. Paper presented at International Symposium of Standardization of
Hematological Methods, Fondazione, Carlo Erba, Milan, Italy, November 9 and 10, 1970.
Symposium proceedings published in Haematologica Latina, 1969.
5. Hamilton PJ and Davidson RL. The interrelationships and stability of Coulter
S-determined blood indices. J Clin Path, 1973; 16:700-705.
6. Bessman JD and Johnson RK. Erythrocyte volume distribution in normal and abnormal
subjects. Blood, 1975; 46:369-379.
7. Price-Jones. The diameter of red cells in pernicious anaemia and in anaemia following
haemorrhage. J Path Bact, 1922; 25:487-504.
8. England JM, Walford DM and Waters DAW. Reassessment of the reliability of the
haematocrit. Brit J Haemat, 1972; 23:247-256.
9. Bull BS, Schneiderman MA and Brecher G. Platelet counts with the COULTER
COUNTER. Am J Clin Path, 1965; 44(6):678-688.
10. Mundschenk DD, et al. An improved technique for the electronic measurement of
platelet size and shape. J Clin Lab Med, 1976; 88(2):301-315.
11. Schulz J and Thom. Electrical sizing and counting of platelets in whole blood. Med Biol
Engr, 1973; 73:447-454.
12. Von Behrens. Mediterranean macrothrombocytopenia. Blood, 1975; 46(2):199-207.
13. Paulus JM. Platelet size in man. Blood, 1975; 46(3):321-336.
14. International Committee for Standardization in Haematology. Recommendations for
reference method for haemoglobinometry in human blood (ICSH Standard EP6/2:1977)
and specifications for international haemiglobincyanide reference preparation (ICSH
Standard EP6/3: 1977) J Clin Path, 1978; 31(2):139-143.
15. Gauthier et al. Human leukocytes: their size distribution and mean corpuscular volume.
Can Med Assn J, 1967; 97:793-796.
16. Hughes-Jones NC et al. Differential leukocyte counts by volume distribution analysis.
Brit J Hem, 1974; 28(1):148.
17. England JM et al. A semi-automatic instrument for estimating the differential leukocyte
count. Biomed Engr, 1975; 10(8):303-304.
18. Wycherley PA and O'Shea MJ. Abridged differential leukocyte counts provided by a
COULTER Channelyzer in a routine haematology laboratory. J Clin Path, 1978;
31(3):271-274.
PN 4237416DB -1
REFERENCES
19. Oberjat TE, Zucker RM and Cassen B. Rapid and reliable differential counts on dilute
leukocyte suspensions. J Lab Clin Med, 1978; 76(3):518-522.
20. Cox C et al. Evalution of COULTER, three part differential system. Am J Clin Path,
1984; 82(3):372-373.
21. Richardson, Jones A. Evaluation of the Coulter histogram differential: a review of the
literature, 1986. Coulter Electronics, Inc., Hialeah, FL
22. Allen JK and Batjer JD. Evaluation of an automated method for leukocyte differential
counts based on electronic volume anlaysis. Arch Pathol Lab Med, 1985;
109(6):534-539.
23. Cornbleet J and Kessinger S. Evaluation of Coulter S-Plus three-part differential in
populations with a high prevalence of abnormalities. Am J Clin Path 1985;
84(5):620-626.
24. Cornbleet J, Kessinger S and Bollinger PB. New data from automated hematology
instruments: technical and clinical applications. ASCP National Meeting, San Francisco,
April, 1987.
25. Greendyke RM et al. A comparison of differential white blood cell counts using manual
technic and the Coulter S-Plus IV. Am J Clin Path, 1985; 84(3):348-350.
26. Griswold D and Champagne VD. Evaluation of the Coulter S-Plus IV three-part
differential in an acute care hospital. Am J Clin Path, 1985; 84(1):49-57.
27. Eckhoff RF. An experimental indication of the volume proportional response of the
Coulter Counter for irregularly shaped particles. J Sci Inst, 1967; 44:648-649.
28. Grover NB, Naaman J, Ben-asson S and Dojanski F. Electrical sizing of particles in
suspension III. Rigid spheroids and red blood cells. Biophys J, 1972; 12:1099-1116.
29. Waterman CS, Atkinson EE, Wilkins B, Fischer CL and Kimsey SL. Improved
measurement of erythrocyte volume distribution by aperture-counter signal analysis.
Clin Chem, 1975; 21:1201-1211.
30. Kachel V and Ruhenstroth-Bauer G. Methodik and Ergebissne Optiseher
Formfatorunter-suchungen bei der Zellvolumenmessung nach Coulter. Micros Acta,
1976; 75:419-423.
31. Luke RG, Koepke JA and Siegel RR. The effect of immunosuppressive drugs and uremia
on automated leukocyte counts. Am J Clin Path 11971; 56:503-507.
32. Koepke JA. Drug interference with leukocyte counting (spurious leukopenia). Drug
Therapy, 1974; 79.
33. Dale NL and Schumacher HR. Platelet satellitism -- new spurious results with automated
instruments. Lab Med, 1982; 13:300-304.
34. Kjeldsberg CR and Hershgold EJ. Spurious thrombocytopenia. JAMA, 1974;
227:628-630.
35. Brittin GM, Brecher G, Johnson CA and Stuart J: 1969. Spurious macrocytosis of
antibody-coated red cells. Am J Clin Path 52:237-241.
-2 PN 4237416DB
REFERENCES
36. Hattersley PG, Gerard PW, Caggiano V and Hash DR. Erroneous values on the
COULTER COUNTER Model S due to high titer cold autoagglutinins. Am J Clin Path,
1971; 55:442-446.
37. Nosanchuk JS, Roark MF and Wanser C. Anemia masked by triglyceridemia. Am J Clin
Path, 1974; 62:838-839.
38. Fales W. Water distribution in blood during sickling of erythrocytes. Blood, 1978;
51:703-709.
39. Richardson-Jones A. Mean platelet volume: clinical utility and the variables of
measurement. 1986; Coulter Electronics, Inc., Hialeah, Florida.
PN 4237416DB -3
REFERENCES
-4 PN 4237416DB
GLOSSARY
Accuracy Ability of the instrument to agree with a predetermined reference value at any point within the
operating range; closeness of a result to the true (accepted) value.
Ambient Surroundings or environment.
Assay Procedure of repeat testing to determine the assigned value for a given lot and level of
control.
Assay Values Values of all parameters in a control established by extensive assay of that control.
Assigned Values Values of all parameters in a calibrator established by extensive testing of that calibrator.
Aspirate-Verify Cycle Aspirates 20 L of whole blood.
Background Count Measure of the amount of electrical or particle interference.
Background Cycle Ensures that instrument is ready to run.
Baud A rate defining how many data bits per second are transferred during communications
between two pieces of equipment.
Blank Cycle Runs diluent through the system to clean it out.
Calibration A procedure to standardize the instrument by determining its deviation from calibration
references and applying any necessary correction factors.
Calibration Factors These are correction factors that the system uses to fine-tune instrument accuracy.
Calibrator A substance traceable to a reference method for preparation or material used to calibrate,
graduate, or adjust a measurement.
Carryover The amount, in percent, of blood cells or Hgb remaining in diluent following the cycling of a
blood sample.
Cell Control A preparation made of human blood with stabilized cells and surrogate material. It is used for
daily instrument quality control.
Clean Baths Cycle You present bleach at the sample probe for aspiration into the baths; alternative to Shutdown.
Cleanup Cycle Cleans up the system during powerup.
Codes On printouts, symbols such as +++++, -----, ....., +, * that appear in place of sample results.
See Heading 9.18, WHAT FLAGS AND CODES MEAN for additional information.
Coefficient of Variation An expression, in percent, of data (SD) spread as related to the mean.
%CV = (SD / mean) 100
Coincidence More than one cell within aperture sensing boundaries at the same time. The system senses
these as one large cell rather than as two distinct cells, so it generates one large pulse.
Control A substance used for monitoring the performance of an analytical process or instrument.
Coulter Histogram How the computer computes absolute numbers for each population of LY, MO, and GR.
Differential (CHD)
Coulter Principle W.H. Coulters method for counting and sizing cells and particles.
Conventions Standard style or format used in a particular manual.
CV (see Coefficient of Variation)
Data Bit Computer code used to transfer each character of information.
Defaults Original settings in the instrument. You can change these to tailor operation to your situation.
Diluter Prepares the proper dilutions for sample analysis.
Dispense Diluent Cycle Provides the proper amount of diluent for preparation of a prediluted sample.
Dispense Lyse Cycle Dispenses lyse into the WBC bath.
PN 4237416DB -1
GLOSSARY
Dispense-Verify Cycle Dispenses proper volume of diluent for preparation of a prediluted sample with 20 L of
whole blood aspirated by the aspirate-verify cycle.
Drain Cycle Drains the RBC bath, WBC bath, and the vacuum isolator chamber.
Dry Prime Diluent Cycle Primes the pickup tube and diluent reservoir. Fills the diluent path between the diluent
container and the diluent reservoir, even if empty; it does not fill the diluent path between the
diluent reservoir and the baths.
Dry Prime Lyse Cycle Primes the lyse path of the fluidics system; fills the lyse path completely, even if empty.
Expiration Date The last day when you can use that lot number of reagent, control or calibrator.
fL Abbreviation for femtoliters.
femtoliters One quadrillionth (10-15) of a liter.
Field Area on a screen for entering data.
Flags On printouts, letters (H, L, *, +) that appear next to parameter results to indicate specific
conditions. See Heading 9.18, WHAT FLAGS AND CODES MEAN for additional information.
Hemoglobinometry Measurement of hemoglobin in the blood. In COULTER instruments, this is done by
comparing the amount of light that passes through a diluted lysed sample in which the
released Hgb has been chemically converted, with the amount of light that passes through a
blank.
Icon Pictorial representation for commands or options on an instrument.
IQAP (Interlaboratory Beckman Coulter provides this program which statistically compares your 4C PLUS cell
Quality Assurance control data to a group of other laboratories' control recovery data.
Program)
Linearity The ability of an instrument to recover expected results (reference values or calculated
values) for such parameters as WBC, RBC, Hgb and Plt at varying levels of concentration of
these parameters within specified limits.
Lot Number A manufacturer's code that identifies when the reagent was manufactured.
Mean Arithmetic average of a group of data.
Operating range Range of results over which the instrument displays, prints, and transmits data.
Outlier Control result that falls outside the expected range.
Parameters Components of blood that the instrument measures and reports.
Parity Method of detecting errors in data handling. The computer generates a parity bit such that the
sum of the data bits and the parity bit are odd or even for each data word.
Performance Actual performance of the instrument.
characteristics
Performance Targeted performance of the instrument based on established ranges and parameters.
specifications
Powerup Cycle Performs appropriate checks to ensure system is functioning correctly and prepares the
instrument for running. This cycle is part of the entire powerup procedure and cannot be
directly selected.
Precision Ability of the instrument to reproduce similar results when a sample is run repeatedly.
Precision of the instrument is a %CV, or an SD for diff parameters, based on at least 31
replicate determinations of the same sample. Precision shows the closeness of test results
when repeated analyses of the same material are performed. A measure of reproducibility.
-2 PN 4237416DB
GLOSSARY
Predilute The process of preparing a minimal amount of blood specimen for analysis by dispensing
diluent to an empty tube then adding the blood specimen.A prediluted sample is different than
a whole-blood sample. See whole blood.
Predilute Cycle Executes request sample analysis using the prediluted specimen.
Prime Sweepflow Cycle Primes the fluidics path from the diluent reservoir through the sweepflow coil and the path
between the RBC aperture and the vacuum isolator chamber.
Prime Timeout Cycle Prepares the Diluter to run samples if Diluter has been idle for 2 hours or more.
Quality Check Cycle Executes Request Sample Analysis using non-labile control as the specimen.
QC (Quality Control) A comprehensive set of procedures your laboratory sets up to ensure that the instrument is
working accurately and precisely.
Reagent Management A program card that manages your reagent usage.
Card
Reproducibility This procedure checks that the system gives similar results (within established limits) every
time it measures the same sample. Also called precision.
Rinse and Mix Cycle Drains the baths, supplies the rinse, and provides the air for mixing.
SD (Standard Deviation) A measure of variation within a group of samples or a population.
Shift Consecutive values that abruptly move from one side of the mean to the other then maintain a
constant level.
Shutdown Cycle Cleans the fluidic lines and apertures to help prevent residue buildup, and turns off Hgb lamp.
Software Card A program card that contains instructions to run the instrument.
Standard Deviation (SD) A measure of variation within a group of samples or a population.
Startup Cycle Ensures that the instrument is ready to run; includes turning on Hgb lamp and performing
background test.
Stop Bit A computer code that indicates the end of a character.
Sweep Flow A steady stream of diluent that flows behind the RBC aperture during sensing periods to keep
RBCs from swirling back into the sensing zone.
TABLE OF EXPECTED Assigned values for a control material used for quality control parameters. Usually reported
RESULTS on a package insert shipped with the control material; can be a separate assay sheet.
Trend Values that continue to increase or decrease gradually over a period of time.
Verification Procedure to analyze cell controls or whole blood with known values to determine if your
control results are within expected range.
Verify Predilute Procedure that performs the aspirate-verify cycle followed by the dispense-verify cycle.
Voting In COULTER hematology instruments, the system compares the three counts for RBC, WBC,
Plt. Unless at least two counts agree, the system does not accept the count. It displays a code
(-----) to indicate a voteout.
Wet Prime Cycle Primes the fluidics path of the Diluter and baths with diluent and removes small amounts of
air that may have leaked into the diluent lines.
Whole Blood Non-diluted blood; blood and anticoagulant only.
Whole Blood Cycle Executes Request Sample Analysis using whole blood as a specimen.
Zap Aperture Cycle Clears the aperture using the zap current circuit.
PN 4237416DB -3
GLOSSARY
-4 PN 4237416DB
ABBREVIATIONS
Abbreviation Explanation
L microliter
m micrometer
A ampere
AIM aperture integrity monitor
ANSI American National Standards Institute
ASCII American Standard Code for Information Interchange
ASTM American Society for Testing and Materials
AWG American Wire Gauge
bps bits per second
CBC complete blood count
CDC Centers for Disease Control and Prevention
CEE Commission for Electrical Equipment
CHD Coulter Histogram Differential
cm centimeter
CSA Canadian Standards Association
CV coefficient of variation
diff differential
dL deciliter
EDTA ethylenediaminetetraacetic acid
FDA Food and Drug Administration
fL femtoliter
ft foot or feet
g gram
gal gallon
GR granulocyte
Hct hematocrit
Hgb hemoglobin
Hz hertz
IEC International Electrical Commission
IQAP Interlaboratory Quality Assurance Program
L liter
LY lymphocyte
m meter
MCH mean corpuscular hemoglobin
MCHC mean corpuscular hemoglobin concentration
MCV mean corpuscular volume
PN 4237416DB -1
ABBREVIATIONS
Abbreviation Explanation
mL milliliter
mm millimeter
MO mononuclear
MPV mean platelet volume
MSDS material safety data sheets
mW milliwatt
n number
NCCLS National Committee for Clinical Laboratory Standards
NEMA National Electrical Manufacturers Association
nm nanometer
pg picogram
Plt platelet
psi pounds per square inch
QA quality assurance
RBC red blood cell
RDW red cell distribution width
SD standard deviation
UL Underwriters Laboratory
Vac volts of alternating current
Vdc volts of direct current
VIC vacuum isolator chamber
VRM Volts Root Mean Square
WBC white blood cell
-2 PN 4237416DB
INDEX
PN 4237416DB 1
INDEX
2 PN 4237416DB
INDEX
PN 4237416DB 3
INDEX
4 PN 4237416DB
INDEX
Print Setup Report, 1-16 downloading 4C PLUS cell control results, 2-14
Printers/Profiles, 1-16 laboratory quality control program, 5-1
Pulse Test, 1-17 irregular sample results
QA screen, 1-16 all parameters, 6-91
Reproducibility, 1-16 aspiration problem, 6-92
Resend to Host, 1-18 aspiration problems, 6-91
Rinse + Mix, 1-17 contamination, 6-91, 6-93
Sample Details, 1-17 diagnostic functions, 6-93
Sample ID Screen, 1-18 diluter functions, 6-92, 6-93
Sample Results Screen, 1-18 dilution problems, 6-92
Save and Exit, 1-18 electrical interference, 6-91, 6-93
setup screen, list of, 1-16 Hgb, 6-92
Solenoids, 1-17 insufficient lytic reagent, 6-91
Transmission, 1-16 insufficient mix bubbles, 6-91
Units, 1-16 MCV, 6-92, 6-93
Verify Predilute, 1-17 Plt, 6-92, 6-93
Voltages/Sensors, 1-17 predilute, 6-91
warning messages, 6-81 RBC, 6-92
Wet Prime, 1-17 sweepflow, 6-93
Zap Apertures, 1-17 vacuum draw, 6-92
icons, main screen
ACT Tron Mode, 1-15
Analyzing Mode, 1-15
L
Darken Screen, 1-15 L flag
Diagnostics, 1-15 action recommended, 6-80
Diluter Functions, 1-15 definition of, 6-80
Lighten Screen, 1-15 Laboratory ID icon, 1-16
Predilute Mode, 1-15 lamps
Sample Results Screen, 1-15 Hgb lamp, location of, 6-9
Setup, 1-15 Hgb, turning ON, 6-81
Shutdown, 1-15 leaks, 6-2
Startup, 1-15 Lighten Screen icon, 1-15
Whole Blood Mode, 1-15 linearity
icons, setup screen definition of, GLOSSARY-2
Calibration Factors, 1-16 lot number
Date/Time, 1-16 definition of, GLOSSARY-2
Exit, 1-16 low counts, 6-91
Patient Limits, 1-16 LV11
Print Setup Report, 1-16 aspiration problem, 6-87
Transmission, 1-16 LV17
Units, 1-16 sample results problem, 6-92
instrument LV4
cleaning the outside of, 6-8 sample results problem, 6-91
components, location of, 6-9 lyse prime, 6-69
shipping, preparing for, 6-63 lytic reagent, 6-91
verifying performance, 6-58 empty, what to do when, 6-83
Interlaboratory Quality Assurance Program. See syringe (1 mL), location of, 6-10
IQAP
IQAP
definition of, GLOSSARY-2
PN 4237416DB 5
INDEX
M tubing, 6-2
maintenance peristaltic pump tubing
general information, 6-1 replacement of, 6-30
procedures, 6-1 pickup tubes
schedule of, 6-1 cleaner, 6-23
why is it important?, 6-1 reagent, 6-14
manuals for your instrument pistons
icons, list of, 1-15 description of, 6-84
using the manuals, 1-11 Plt
MCV, 6-76, 6-92, 6-93 only incorrect parameter, 6-93
MCV +++++ Plt+
description of, 6-76 action recommended, 6-79
MCV+++++ meaning of, 6-79
action recommended, 6-76 PM1
mean peristaltic pump, 6-83
definition of, GLOSSARY-2 PM2
messages, fatal. See fatal errors pump, diluent peristaltic, 6-82
messages, warning. See warning messages power problems, 6-86
motors cord, 6-86
horizontal traverse, location of, 6-9 defective power switch, 6-86
Motors icon, 1-17 instrument malfunctions, 6-86
troubleshooting, 6-86
voltage, 6-86
N powerup cycle
NEEDED definition of, GLOSSARY-2
calibration parameters, 5-16 precision
definition of, GLOSSARY-2
predilute cycle
O definition of, GLOSSARY-3
outlier predilute mode
definition of, GLOSSARY-2 procedures for running sample, 3-5
running samples in, 3-5
verifying predilute, 6-71
P Predilute Mode icon, 1-15
parameters Prepare to Ship icon, 1-17
definition of, GLOSSARY-2 preventive maintenance
parity procedures, 6-1
definition of, GLOSSARY-2 prime, 6-13, 6-18, 6-93
PASSED diluent, 6-69, 6-82
calibration parameters, 5-16 lyse, 6-69
Patient Limits icon, 1-16 sweepflow, 6-69
patient results wet, 6-69
Storage icon, 1-18 prime sweepflow cycle
PCMCIA Error, 6-84 definition of, GLOSSARY-3
performance characteristics Prime Sweepflow icon, 1-17
definition of, GLOSSARY-2 prime timeout cycle
performance specifications definition of, GLOSSARY-3
definition of, GLOSSARY-2 Print Sample Results icon, 1-18
peristaltic pump Print Setup Report icon, 1-16
description of, 6-83
6 PN 4237416DB
INDEX
R S
R flag sample
definition of, 6-74 details reviewing, 6-71
RBC bath, 6-93 mixing, 6-91
location illustration, 6-9 transmission of results, 6-81
location of, 6-9 Sample Details icon, 1-17
RBC+ sample ID
action recommended, 6-79 icons for screen, 1-18
definition of, 6-79 verifying, 3-2, 3-6
reagent management card sample results
definition of, GLOSSARY-3 icons, screen, 1-18
reagents, 6-3, 6-82 Sample Results Screen icon, 1-15
ACT Rinse shutdown diluent, replacement of, samples
6-23 how to run, 3-1
diff ACT PAK, replacement, 6-13 parameter criteria, reproducibility, 5-4
diff ACT Tainer replacement, 6-18 running in predilute mode, 3-5
lytic, 6-91 running in whole-blood mode, 3-1
opening a new container, 6-18 Save and Exit icon, 1-18
priming, 6-18 S-CAL calibration
references in manual kit, function of, 5-10
PN 4237416DB 7
INDEX
8 PN 4237416DB
INDEX
PN 4237416DB 9
INDEX
Z
zap aperture cycle
definition of, GLOSSARY-3
zap apertures, 6-70
Zap Apertures icon, 1-17
10 PN 4237416DB
TRADEMARKS
ACT diff, ACT Rinse, ACT Tron, the Beckman Coulter logo, COULTER, diff ACT Pak,
diff ACT Tainer, 4C and S-CAL are trademarks of Beckman Coulter, Inc.
All other trademarks, service marks, products, or services are trademarks or registered
trademarks of their respective holders.
PN 4237416DB
COULTER ACT diff Analyzer Documentation