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BC 5000 5150
BC 5000 5150
SERVICE MANUAL
Introduction
Before starting to service this instrument, please read this manual thoroughly for proper
service.
After reading this manual, make sure it is well kept so that it is available when needed.
2012 Shenzhen Mindray Bio-medical Electronics Co., Ltd. All rights Reserved.
Microsoft Corporation owns the copyright of SQL SERVER 2005 EXPRESS EDITION. 2005
Microsoft Corporation. All rights reserved.
Statement
Mindray is responsible for safety, reliability and performance of this product only in the
condition that:
all installation operations, expansions, changes, modifications and repairs of this
product are conducted by Mindray authorized personnel;
all replacement parts and supporting accessories and consumables involved in the
service are original Mindray parts or Mindray authorized parts;
the relevant electrical installation complies with the applicable national requirements;
Repair Service
Free Service:
Free service is provided for any product within the scope specified by Mindray warranty
rules.
Paid Service:
Mindray offers paid service for any product beyond the scope specified by Mindray
warranty rules;
even during the warranty period, only paid service is available if the need for service is caused
by the following reasons: artificial damage; improper use; grid voltage beyond the specified
range of the device; irresistible natural disasters; replacement with parts and consumables not
authorized by Mindray or service work by personnel not authorized by Mindray.
Return Procedure
In the event that it becomes necessary to return this product or part of this product to
Mindray, the following procedure should be followed::
Obtain return authorization: Contact the Mindray Service Department and obtain a
Customer Service Authorization (Mindray) number. The Mindray number must appear
on the outside of the shipping container. Returned shipments will not be accepted if the
Mindray number is not clearly visible. Please provide the model number, serial number,
and a brief description of the reason for return.
Freight policy: The customer is responsible for freight charges when shipping the
product to Mindray for service (including customs charges).
Company Contact
Manufacturer:: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
Address: Mindray Building, Keji 12th Road South, Hi-tech Industrial Park, Nanshan, Shenzhen
Website: www.mindray.com
II
This system is only intended for use by qualified service personnel trained by
Mindray or Mindray's agents.
It is important for the hospital or organization that employs this equipment to
carry out a reasonable service/maintenance plan. Neglect of this may result in
machine breakdown or injury of human health.
Be sure to operate the analyzer under the situation specified in this manual;
otherwise, the analyzer will not work normally and the analysis results will be
unreliable, which would damage the analyzer components and cause personal injury.
This manual is only provided for qualified service personnel trained by Mindray or
Mindray's agents.
III
Table of Contents
1
Table of Contents
2
Table of Contents
3
Table of Contents
4
Table of Contents
5
Table of Contents
11 Troubleshooting ........................................................................................................11-1
12 Debug........................................................................................................................ 12-1
12.1 Mechanical Position Adjustment ................................................................................. 12-1
12.2 Commissioning of detection elements ........................................................................ 12-2
12.2.1 Calibrate and verify the preheat temperature ................................................. 12-2
12.2.2 Counting channel measurement .................................................................. 12-3
6
Table of Contents
7
1 Overview
1.1 Overview
This chapter describes how to use the service manual. In this manual, the repair methods
of BC-5150/BC-5000 are described in detail. Before servicing BC-5150/BC-5000, please carefully
read and understand the content in order to properly carry out equipment maintenance and
ensure the safety of service personnel.
This manual must be used in conjunction with the BC-5150/BC-5000 Operators manual. It
does not contain information and procedures already covered in the Operators manual of
BC-5150/BC-5000.
Be sure to operate and service the analyzer strictly as instructed in this manual and the
operators manual.
1-1
Overview
Format Meaning
[] all capital letters enclosed in [ ] indicate a key name
(either on the pop-up keyboard or the external keyboard)
letters included in " " indicate text you can find on the
screen of BC-5150/BC-5000
italic letters indicate titles of the chapters that are referred
to
All illustrations in this manual are provided as examples only. They may not necessarily
reflect your analyzer setup or data displayed.
1-2
Overview
All the substances (samples, controls, calibrators, reagents and liquid wastes)
and areas in contact with these substances are potentially infectious. Wear proper
personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory
procedures when accessing these substances and areas in the laboratory.
In the event of main unit leak, the leaking fluid is biohazardous.
WARNING
It is important for the hospital or organization that employs this equipment to carry out a
reasonable service/maintenance plan. Neglect of this may result in machine breakdown or
injury of human health.
Never use combustible gas (e.g. anesthetic) or combustible liquid (e.g. ethanol) around the
analyzer. Otherwise, the risk of explosion may exist.
When servicing the analyzer, be sure to turn off the power. Servicing the analyzer when it
is on may bring risk of electric shock or damage to electronic components.
Please connect the analyzer to a socket having sole fuse and protective switch. Do not use
the same fuse and protective switch with other equipment (e.g. life supporting equipment).
Otherwise, the equipment failure, over current or impulse current that occurs at the startup
moment may lead to tripping.
To prevent personal injury during the maintenance, keep your clothes, hairs and hands
from the moving parts, such as sample probe, pincher and piercer.
Possible mechanical movement of the warned position may lead to personal injury during
normal operation, removal and service verification.
Be sure to dispose of reagents, waste, samples, consumables, etc. according to government
regulations.
1-3
Overview
The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective
equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when
handling them in the laboratory.
If the reagents accidentally spill on your skin, wash them off with plenty of water and if
necessary, go see a doctor; if the reagents accidentally spill into your eyes, wash them off
with plenty of water and immediately go see a doctor.
CAUTION
Improper maintenance may damage the analyzer. Maintain the analyzer strictly as
instructed by the service manual and inspect the analyzer carefully after the maintenance.
For problems not mentioned in the service manual, contact Mindray customer service
department for maintenance advice.
To prevent personal injury or damage to equipment components, remove metal jewelry
before maintaining or servicing electronic components of the equipment.
Electrostatic discharge may damage electronic components. If there is a possibility of ESD
damage with a procedure, then do that procedure at an ESD workstation, or wear an
antistatic wrist strap.
NOTE
The operator is required to follow the instructions below this symbol. The
instructions will emphasize important information or information that requires
particular attention of the operator.
1.6 Symbol
Symbols used in this service manual:
Symbol Meaning
The operator is required to follow the instructions below
this symbol. Failure to do so may place the operator at a
potential risk of biohazard.
The operator is required to follow the instructions below
WARNING this symbol. Failure to do so may cause personal injury.
1-4
Overview
CAUTION
Ensure the labels are in good condition and not damaged while servicing the analyzer.
USB port
Network interface
ALTERNATING CURRENT
Batch code
1-5
Overview
USE BY (YYYY-MM-DD)
Serial number
MEASUREMENT AUTHORIZATION
SYMBOL
DATE OF MANUFACTURE
Pricking danger
Manufacturer
TEMPERATURE LIMITATION
1-6
2 Specifications
BC-5150 Appearance
BC-5000 Appearance
2-1
Specifications
Height
Depth
Width
BC-5150/BC-5000 Overall
Width 325 mm
Depth 410 mm
Weight 25Kg
Parameter Value
Voltage (100V-240V~) 10%
Input Power 300VA
Frequency 50/601Hz
2-2
Specifications
2.1.4 Specifications
Measurement mode
Two measurement modes are provided: CBC and CBC+DIFF.
Sample mode
Three sample modes are provided: whole blood mode, prediluted mode and Capillary Whole
Blood mode.
Each of the three sample modes can be used in both CBC and CBC+DIFF measurement mode.
Measurement speed
BC-5000's testing speed for OV-WB/OV-PD/OV_PWB modes is not lower than 40 samples
per hour.
BC-5150's testing speed for OV-WB/OV-PD/OV_PWB modes is not lower than 60 samples
per hour.
2-3
Specifications
Plateletcrit PCT
Platelet larger cell ratio P-LCR
Platelet larger cell count P-LCC
Histogram
English Name Abbreviation CBC CBC + DIFF
White Blood Cell Histogram WBC Histogram
Red Blood Cell Histogram RBC Histogram
2-4
Specifications
Scattergram
Name Abbreviation CBC CBC + DIFF
Differential Scattergram Diff Scattergram /
HGB 1g/L
HCT 0.5 %
PLT 10 109 / L
2.3.2 Carryover
Carryover refers to the transfer of blood cells from high concentration sample to low
concentration sample.
Verification method:
Prepare a high concentration sample (centrifugated high value control or
2-5
Specifications
special high value linearity control) which is within the range specified in Table
13, mix and then test it for 3 consecutive times, the test results are i1, i2 and
i3; prepare a low concentration sample (diluted low value control, dilution ratio:
1:10) which is within the range specified in Table 13, test it for 3 consecutive
times, the test results are j1, j2 and j3. Calculate the carryover according to
the following equation, the result shall meet the requirements in Table 12.
Parameter Carryover
WBC 0.5
RBC 0.5
HGB 0.6
HCT 0.5
PLT 1.0
2.3.3 Repeatability
Test a sample which meets repeatability requirement on the analyzer for 10 consecutive times,
calculate the CV(%) and absolute deviation (d) of each parameter, the results shall meet the
requirements in the following table.
In the equation:
s ---- standard deviation of sample test results;
x ---- mean value of sample test results;
2-6
Specifications
2-7
Specifications
The illustrations in this manual are based on BC-5150. The structures of BC-5000
and BC-5150 are basically the same.
2-8
Specifications
This analyzer is heavy and may cause personal injury if handled by only one
person. If necessary, it is recommended to use two person for handling the analyzer. It
is important to follow appropriate safety rules and use appropriate tools while
handling.
2-9
Figure 2-1 Front view of the main unit
2-10
Specifications
2-11
Specifications
Main unit
The machine for analysis and data processing is the main part of the product.
Power/Status indicator
The Power/Status indicator is located in the middle of the right side of the analyzer (front
side). It tells you about the status of the analyzer including ready, running, error, sleep and
on/off, etc.
Power switch
The power switch is located on the rear side of the main unit. This switch can be used to
turn on/off the main unit.
To avoid damage, do not turn on/off the power of the analyzer continually in a short
time.
2-12
Specifications
[Aspirate] key
The [Aspirate] key is located on the middle of the right front side (the right one). You can
press the key to start the selected analysis cycle, dispense diluent or wake up the system from
sleep
USB port
There are 4 USB ports on the left side of the main unit for peripheral connection or data
transmission.
Store and use the reagents as instructed by instructions for use of the reagents.
When you have changed the diluent, lyses or cleansers, run a background to see if the
results meet the requirement.
Pay attention to the expiration dates and open-container stability days of all the
reagents. Be sure not to use expired reagents.
After installing a new container of reagent, keep it still for a while before use.
2-13
Specifications
2.7.1 Reagents
M-52 D DILUENT
Used for diluting the blood samples to achieve functions such as blood cell counting,
volume measurement and hemoglobin measurement.
Used for lysing red blood cells to categorize quartile groups of white blood cells.
M-52 LH LYSE
Used for lysing red blood cells to achieve functions such as white blood cell counting,
classification of basophils and hemoglobin measurement.
PROBE CLEANSER
2-14
Specifications
Storage BC-5000's storage capacity of sample data is not less than 20000
Capacity BC-5150's storage capacity of sample data is not less than 40000
Storage The storage contents shall include at least the following
Contents information: counting results and diagrams (including histograms
and scattergrams), sample information, patient information,
alarm message, special information of the instrument
2-15
3 Operation Principles
3.1 Overview
This analyzer employs Coulter principle to test the number of RBC and PLT, colorimetric
method to measure the hemoglobin concentration, and semiconductor laser flow cytometry
to obtain differential statistics of white blood cells. The analyzer will calculate the other
parameters based on these results.
3.2 Workflow
The whole system contains the following main functions: reagent system, sample allocation,
sample preparation, sample testing, signal processing, parameter analysis, data management,
status monitoring, scheduling control and information processing, human machine interface,
power supply, cleaning and maintenance. The relationship between these functions are as
shown in the chart below.
The scheduling control and information processing function block controls other function
blocks, which collaborate in accordance with the designed processes and requirements to
3-1
Operation Principles
complete the core task of the whole system, i.e. sample measurement and analysis.
After the blood sample is mixed by the lyse, the red blood cells will be lysed, and the white
blood cells will be dyed. Through the sample probe, the dyed fragments of white blood cell and
red blood cell are injected into the flow cell, which is filled with the diluent. Wrapped in the
sheath fluid formed by the diluent, the cells go through the laser detection zone in rows after a
secondary acceleration. When the cells are exposed to laser beam, the scattered light is related
to the cell size and the refractive index of both the cell membrane and the internal structure.
These scattered light signals are received and converted into electrical pulses by the photodiode.
From these electrical pulses, a two-dimensional distribution map of the cell size and internal
information and be obtained, which is called a scattergram. From the WBC scattergram and
histogram, the white blood cell differential and count can be obtained.
3-2
Operation Principles
Colorimetric method
After the diluted sample is added into the lyse, the red blood cells will lyse and release
hemoglobin, which combines the lyse to form hemoglobin complexes. According to the
Lambert-Beer's law, with the radiation of LED monochromatic light with a central wave length of
530 nm, it is possible to measure the transmitted light density of the hemoglobin complexes in
the solution and background, by which the hemoglobin concentration can be calculated.
Blank Photocurrent
HGB(g/L) = Constant Ln
Sample Photocurrent
Red blood cell/Platelet measurement
Impedance method
This analyzer employs the impedance method to count the RBC/PLT. There is a small
opening in the RBC bath, which is called inspection aperture. A pair of electrodes on both sides
of the aperture are connected to a constant current power supply. Since the cells are poor
conductor of electricity, when the cells in the diluted sample pass through the aperture under a
constant negative pressure, the resistance between the electrodes changes to generate a pulse
signal across the electrodes, which is proportional to the cell size. The number of the pulses is
equal to the number of cells that pass the aperture, and the amplitude of the pulses is
proportional to the cell size.
3-3
Operation Principles
The collected electric pulses are amplified and then compared with the channel voltage
threshold corresponding to the size range of normal red blood cells/platelets, in order to
calculate the number of the pulses which amplitude are within the red blood cell/platelet
channel. Therefore, the collected electric pulses are classified according to the channel voltage
threshold. The numbers of the electric pulses within the red blood cell/platelet channel are the
numbers of the red blood cells/platelets. The size distribution of the cells is determined by the
numbers of the cells in each channel, which are classified according to the pulse voltage
amplitude. The two-dimensional diagram, in which the horizontal axis represents the cell size
and the vertical axis represents the relative number of the cells, are the histogram that reflects
the distribution of the cell groups.
Time Measurement
The basis of time measurement is under certain vacuum pressure and
with fixed aperture dimension, the sample volume passes through the
aperture within a certain period of time is definite. Thus analysis
results can be obtained by calculating sample volume from analysis
duration, dilution ratio, etc. And analysis accuracy can be ensured by
controlling vacuum generating process and monitoring real-time vacuum
pressure and aperture voltage.
Monitor and determine clogging: by monitoring aperture and sample
particle information, relevant characteristic information is concluded,
and the judgment is made in combination with the threshold.
3-4
Operation Principles
RBC (1012/L) is the number of erythrocytes measured directly by counting the erythrocytes
passing through the aperture.
Mean red blood cell volume
Based on the RBC histogram, this analyzer calculates the mean cell volume (MCV) and
expresses the result in fL.
RBC MCV
HCT =
10
HGB
MCH =
RBC
HGB
MCHC = 100
HCT
where the RBC is in 1012/L, the MCV is in fL, and the HGB is in g/L.
Platelet parameters
Platelet count
PLT (109/L) is measured directly by counting the platelets passing through the aperture.
Plateletcrit
3-5
Operation Principles
This analyzer calculates the PCT as follows and express it in , where the PLT is expressed
in 109/L and the MPV in fL.
Flag
Flag Meaning Judgment criterion
Type
Interference of PLT clump or 1. The DIFF and BASO
WBC Abnormal NRBC to WBC count and channels are
differential may exist: unproportionate.
Many scatter-points in the
Immature cells or blasts may
Immature Cell? immature cell area of the
exist
scattergram
Many scatter-points in the
Abnormal lymphocytes or
abnormal/ atypical
Abn./Atypical Lym? atypical lymphocytes may
lymphocytes area of the
exist.
scattergram
Leucopenia Low WBC analysis results WBC < 2.5010^9/L
Leucocytosis High WBC analysis results WBC > 18.0010^9/L
Low neutrophils analysis
Neutropenia NEUT# < 1.0010^9/L
results
WBC
High neutrophils analysis
Neutrophilia NEUT# > 11.0010^9/L
results
Low lymphocytes analysis
Lymphopenia LYMPH# < 0.8010^9/L
results
High lymphocytes analysis
Lymphocytosis LYMPH# > 4.0010^9/L
results
High monocytes analysis
Monocytosis MONO# > 1.5010^9/L
results
High eosinophils analysis
Eosinophilia EO# > 0.7010^9/L
results
Basophilia High basophils analysis results BASO# > 0.2010^9/L
Wbc < 4.010^9/L and Rbc <
Pancytopenia WBC, RBC and PLT low 3.512^9/L and Plt < 100
10^9/L
Possible presence of The distribution of RBC
RBC RBC Histogram Abn.
microcytes, macrocytes, histogram is abnormal
3-6
Operation Principles
anisocytosis, RBC
agglutination and dimorphic
histogram
HGB abnormal or RBC
MCHC > 380 g/L
HGB Abn./Interfere? agglutination, or interference
or HGB interference
may exist (e.g., WBC high)
Microcytosis MCV low Mcv < 70fL
Macrocytosis MCV high Mcv > 110fL
Anemia Anemia HGB < 90g/L
Erythrocytosis RBC high RBC > 6.510^12/L
Possible presence of
The distribution of PLT
PLT Scattergram Abn. microcytes, red blood cell
scattergram is abnormal
debris, giant PLT or PLT clump
PLT
Thrombopenia PLT low PLT < 6010^9/L
Microcytosis /
Macrocytosis /
3-7
Operation Principles
Anemia /
Erythrocytosis /
PLT Scattergram Abn. R/Flag parameter: PLT, MPV, PDW, PCT, PLCR, PLCC, etc.
PLT
Thrombopenia /
Thrombocytosis /
3-8
4 Software and Interface
4.1. Start-up
Version Check
At startup, the machine will automatically check the software version. If the software version
does not match, a window will pop up as shown below:
4-1
Software and Interface
4.2. Login
4.2.1 Log in using service level username and password
Username: "Service"
Password: "Se s700"(there is a space between Se and s700).
4-2
Software and Interface
4-3
Software and Interface
4.3. Review
4-4
Software and Interface
4.4. Calibration
4-5
Software and Interface
There are two different analysis modes, CBC+DIFF and CBC. The two analysis modes
respectively correspond to two fluidics sequence. Therefore, the analysis results of the same sample in
different modes are different. However, this difference is relatively fixed. During calibration, it is
only required to obtain the calibration factor of one mode. The calibration factor of the other mode
can be calculated by multiplying this fixed difference coefficient, which is called the transfer factor.
The calculation equation of the transfer factor is:
There are two different sample modes, whole blood mode and prediluted mode, which also
correspond to different fluidics sequence. Therefore, different sample modes need to be calibrated
separately.
The calibration factors can be classified as factory calibration factor and user calibration factor.
For the CBC+DIFF mode, the analysis result will be calculated by the following equation:
If login with service level password, the calibration will modify the factory
calibration factor and transfer factor, and will modify the user calibration factor to
100.00%.
4-6
Software and Interface
4.4.2 Calibration
Please use specified calibrators for calibration before their expiration date.
If the calibration factor and CV are beyond the above range, they will be displayed in
red, and the current result will not be saved.
4-7
Software and Interface
4-8
Software and Interface
4-9
Software and Interface
4-10
Software and Interface
Please use specified calibrators for gain calibration before their expiration date.
For the targets, please refer to the calibrator target sheet.
The gain factor is a percentage value. The Gain Setting screen allows the gain factor to be set as
accurate to two decimal places.
The MCV gain and HGB gain are software gains, which require the digital potentiometer to be
set. The range of gain setting is [0, 255].
The MCV gain setting can be calculated by the following equation:
4-11
Software and Interface
The HGB gain setting does not need to be calculated by an equation. Just modify the
setting until the background voltage is equal to 4.5V.
The gain settings will have effect on the affectivity of the measurement. Please be
careful with the setting.
While the analyzer is in standby, the HGB voltage will not reflect the background
voltage. In this event, the operator must exit the standby mode before adjusting the HGB
gain.
4.9. Performance
4-12
Software and Interface
4.9.2 Reproducibility
Normal controls are usually used for reproducibility application at the client end.
4-13
Software and Interface
4.9.3 Carryover
4-14
Software and Interface
The modification to language type will not take effect until after the analyzer is
restarted.
The saved inf files and raw data files will occupy relatively large amounts of disk
(SD card) space.
4-15
Software and Interface
At most 500 inf files can be saved. After 500 files are saved, the new files will
overwrite the old files.
Inf files
Software debug information: includes parameter setting, error log, upgrade log and system
log.
4-16
Software and Interface
Maxell 4/8G.
There is enough free space in the USB drive. It is recommended to reserve 4G space.
Unzip update.rar and copy the unzipped update directory to the root directory of the
USB drive.
Upgrade
Insert the USB drive into the USB port on the analyzer. Enter the Advanced Toolbox
and launch Upgrade to upgrade the software according to the prompts. The upgrade process can
be divided into two steps.
When upgrading the boot and operation system, the operator will be prompted to
restart the analyzer between step 1 and step 2. When upgrading the software components, the
upgrade process will directly go to step 2.
Never disconnect the USB drive or the power supply during the upgrade process.
Otherwise the analyzer may not be able to start.
The duration of the upgrade process varies with the upgrade contents. Typically it
will last for around 10 minutes. Interaction is needed during the process. Please do
not leave when upgrading the analyzer.
4-17
Software and Interface
Troubleshooting
4.13. Buzzer
When an error occurs, the buzzer will beep. The alarm will be automatically cleared by tapping
the touchscreen or correcting the error. The buzzer alarm will stop when all the error are cleared. It
prompts to instruct the user with possible actions by the beep.
Table 4-2 Main unit buzzer prompts
Event Buzzer Comments
Prompts
Startup completed One short beep Startup completed means the
whole startup process has been
completed and the analyzer is
ready for operation
Open-vial aspiration completed Two short beeps
When count operation can not be One long beep If these screens have already
4-18
Software and Interface
4-19
5 Data Transmission
5.1. LIS Connection
Communication setup (Menu>Setup>System Setup>Communication Setup)
The operator can perform the following setups in the Communication Setup screen
Protocol setup
Transmission mode
IP address:
The IP address setting of the analyzer defaulted as 10.0.0.2
Subnet mask:
The subnet mask of the analyzer. A typical subnet mask is 255.255.255.0
Default gateway:
IP address of the gateway.
Mac address:
The Mac address of the analyzer, given by the factory. Can not be changed.
Comm. Protocol:
For selecting the protocol type. Click the pull-down list and select the appropriate
communication protocol type from the options.
5-1
Data Transmission
The IP address of the analyzer is statically allocated. Before setup, please consult
your network administrator to avoid IP conflict.
For communication across subnets, the subnet mask and the gateway must be correct.
Please consult your network administrator.
Transmission mode
The operator can select required options by clicking the following checkboxes to activate
corresponding communication setup as needed:
Auto retransmit
The Auto retransmit can only be selected when the ACK synchronous
transmission is selected, if ACK is not selected after overtime, the software will
transmitted the content that has transmitted before automatically.
Auto communication
If it is selected, the software will transmit the sample information and count
result to the LIS automatically after the sample analysis.
Transmit as print bitmap data
If it is selected, the scattergram and histogram that transmitted to the LIS are the
same as the print graph, whose background are white.
Transmission method for histograms and scattergrams
Click the pulldown list and select the transmission method for histograms and scattergrams
as required from the following options:
Not transmitted
If it is selected, the graphic and image data will not be transmitted.
Bitmap
If it is selected, the image data will be transmitted, all the scattergram and
histogram of the LIS received are bitmap.
Data
If it is selected, the graph data will be transmitted during the transmission, the
scattergram and histogram that LIS received is the data, the data format is customized;
it can only be viewed by the Mindray data management software.
5-2
Data Transmission
Figure 5-2 Communication Parameter Setup screen for data management software
As illustrated above, IP Address refers to the analyzer IP address, Port is fixed as 5100, and
Protocol shall match the analyzer configuration.
Communication instrument management
5-3
Data Transmission
Figure 5-3 Communication Instrument Management screen for data management software
The analyzer is the server, and the LIS and data management software is the client. The
connection needs to be initiated by the client.
5-4
6 Optical System
Since the sample stream has a certain width, different cells will pass the beam zone at slightly
different positions. In order to ensure the consistency of cell scatter signals, the Gaussian beam shall
has a certain width in the direction perpendicular to the cell movement to minimize the density
variation of the beam coving the sample stream, as shown in Figure 6-1. Meanwhile, in order to
prevent the beam from radiating several cells at the same time, the beam shall be small enough in the
direction of the cell movement, just being able to cover the entire cell. Therefore, the beam used for
radiating the sample stream shall be an elongated elliptical beam.
6-1
Optical System
Light source assembly: Beam shaping function component, which shapes the divergent elliptical
beam generated by the semiconductor laser to elongated beam and directs it into the flow cell.
Flow cell assembly: Both an optical component and a fluidic component. As the fluidic interface
of the optical system, it provides stable sample streams. The optical performance of this component is
also critical. Dirt, contamination or dust in the optical zone on the inside and outside surface of the
flow cell may have great impact on the performance of the optical system.
Scattering detection assembly: consists of aperture and photoelectric sensor. Used for collecting
the scattered light generated by the cells. There are three photoelectric sensors in the optical system
for collecting scattered light of three angle ranges, including Low Angle Scatter (LAS), Medium
Angle Scatter (MAS) and Wide Angle Scatter (WAS).
6-2
Optical System
6-3
Optical System
(a) Physical view of the flow cell assembly (b) Operation principle of the flow cell
6-4
Optical System
Both the removal of internal parts of the flow cell assembly and the removal of the assembly
from the substrate are forbidden. Generally, if the flow cell assembly is determined to be faulty, the
whole optical system shall be replaced.
6-5
Optical System
6-6
Optical System
large angle aperture and support structure. The 3D model and physical view are shown in Figure 6-10.
The scattering assembly is a whole unit and can not be removed from the optical substrate, although
the large angle PD assembly is removable. When the scattering detection assembly is determined to
be faulty, it is necessary to replace the whole optical system.
6-7
Optical System
part. bottle until the solution is well mixed, then add 3 drops of the solution in to the
1.5mL centrifuge tube. Cap the tube and shake it until this solution become well
mixed, as shown in Figure 6-12.
2. Select the Maintenance menu and enter the Optical screen. Perform counting with
the prepared std. part. solution. After the counting is completed, the result will be
automatically displayed in the screen, as shown in Figure 6-13.
3. Determine the optical system status by the parameter Particle 1 according to the
counting result. The optical system is OK if all the following requirements are met:
Parameter Total CG Position CV
LAS 38~45 6.50
MAS 1500~3000 100~158 3.00
WAS 100~200 8.00
4. Generally, if the parameters fail to meet the requirements, it may be caused by a dirty
flow cell. The built-in maintenance program can be used to solve this problem. The
method is as follows: click the Maintenance-->Maintenance menu and select Flow
Cell Cleaning in the Cleaning screen. The machine will automatically complete the
cleaning operation (in about 1 minute).
After the maintenance, repeat step 2 and 3 and check if the result meets the requirements. If not,
probe cleanser should be used to clean the flow cell: click the Maintenance-->Maintenance menu
and select Overall Soak in the Maintenance screen, and carry out the operation of the program.
After the maintenance above, repeat step 2 and 3 again, and check if the result meets the requirements.
If still not, the manual maintenance is required. For details, please see the next chapter.
6-8
Optical System
6-9
Optical System
Tap "Status" > "Voltage&Current" in the menu to enter the Voltage&Current screen, the
laser will illuminate automatically.
Never look directly into the laser with eyes or through an optical instrument;
During test of optical system with the top cover open, please shelter the optical system to
prevent bright environmental light from irradiating the detector inside the optical system.
Generally, the following steps can be followed to determine which part needs to be maintained.
1. Check if the wires are firmly connected inside the optical system and if the optical path is
blocked by wires.
2. Check if the output spot is normal. Place a small piece of white paper near the output exit
and observe the light spot. The ideal spot shape is a vertical ellipse which is clipped at both
top and bottom, as shown in the left in Figure 6-15. The actual spot is shown in the right in
Figure 6-15 with a faint halo around it.
6-10
Optical System
Abnormal spots can be different, including dark spot, spot clustered into a dot, spot with dark
lines, spot with a scattered halo, seriously damaged spot, spot with multiple dark dots in the center, as
shown in Figure 6-16.
Abnormal light spots are usually caused by damage or contamination of the laser control board,
the laser or the lens in the light source assembly.
Missing parts, dark lines or dark dots in the spot are usually caused by contaminated lens in the
light source assembly. A clean cloth dampened with dehydrated alcohol may be used to wipe the lens
gently, spiraling outward from the center. Be careful not to touch the interior of the lens barrel.
If the light spot disappears, darkens or diverges, then the laser control board may be damaged.
After the problem is determined, replace the laser control board (be aware of electrostatic) separately
and adjust the variable resistor on the board. Perform a std. part. test in accordance with 6.3 to ensure
the std. part. indicators to meet the requirements. After replacement, a gain calibration in accordance
with Section 4.6 as well as a calibration in accordance with Section 4.3 shall be performed on the
optical system.
6-11
Optical System
If the light spot disappears, converges, darkens or diverges, and the laser control board is
determined to be in good condition, then the laser could have been burned. Since the light source
assembly can not be replaced separately, it will be necessary to replace the optical system.
3. Observe the light spots on the surface and inside the flow cell from the following angles, as
shown in Figure 6-17. If the exterior of the flow cell is very bright, then the exterior may be
stained. Use a clean cloth dampened with dehydrated alcohol to wipe the exterior, until the
bright part darkens or disappears, as shown in the right in Figure 6-17. If the interior of the
flow cell is very bright and can not be darkened by the built-in cleaning procedure, then it
will be necessary to manually rinse the inside of the flow cell.
The cleansing procedure is as follows: Prepare a syringe with a 100 mm long Teflon
tube, a 200 mm long Teflon tube and probe cleanser, as shown in Figure 6-18. Shutdown
the analyzer, remove the tubes at the waste outlet and the sheath inlet of the flow cell.
Connect the syringe to the waste outlet. Connect one end of the other Teflon tube to the
sheath inlet, and put another end into the probe cleanser. Draw the probe cleanser with the
syringe until the probe cleanser enters the syringe. At this time, the flow cell will be filled
with the probe cleanser. After soaking for about 10 minutes, use clean water instead of the
cleanser. Draw the syringe forcefully for 2 to 3 times, until the bright spots in the flow cell
darken or disappear.
Connect the optical path and the tubes and verify the connection, and then turn on the
analyzer power.
6-12
Optical System
4. Check if the output light is perpendicular to the flow cell. Place an inner hexagon spanner
at the output exit. The output spot and the spot reflected by the flow cell shall appear
simultaneously and strictly coincide with each other, as shown in Figure 6-19.
If the std. part. CV meets the requirement, but the CG position is too low or too high,
slightly adjust the variable resistor on the laser control board as shown in Figure 6-11, until
the CG position meets the requirement. After maintenance according to this procedure, a
gain calibration in accordance with Section 4.6 as well as a calibration in accordance with
Section 4.3 shall be performed on the optical system.
If the CG position meets the requirement but the CV doesn't, then the maintenance is not
qualified. Perform another check and maintenance in accordance with this section.
If the CG position and the CV can not meet the requirements after the maintenance, then it
will be necessary to replace the optical system.
6-13
Optical System
3. Remove the optical system carefully and short the tubes of the optical system in accordance
with Figure 6-21.
6-14
Optical System
4. Install the new optical system into the analyzer. Connect the signal wires, fluidics and
optical path. Turn on the analyzer and verify the status of the new system in accordance
with the steps in Section 6.3.
Note: The fluidic interfaces of the optical system are very fragile. Be careful to avoid
knocking them when connecting the tubes during installation!
6-15
7 Fluidics
7.1. Measurement Flow
The fluidics of the analyzer can be divided into two measurement channels:
WBC&HGB Channel
RBC&PLT Channel
The system flowchart of the WB-CBC+DIFF mode is shown below (DIFF lyse and DIFF
sample configuration & measurement are not available in CBC mode):
7-1
Fluidics
The system flowchart of the PD-CBC+DIFF mode is shown below (DIFF lyse and DIFF sample
configuration & measurement are not available in CBC mode):
7-2
Fluidics
DIFF reagent: used for lysis of red blood cells and specialization of different white blood
cells;
Diluent: background solution used for providing sheath fluid and cleansing
Measurement principle: flow cytometry and laser scatter
Counting volume: the flow of the sample stream is constant, which can be
converted to counting volume by controlling the counting duration
LH lyse: used for lysing red blood cells and platelets and separating the basophiles by
volume from the other white blood cells
Diluent: used for providing sheath fluid and cleansing
Measurement principle: flow cytometry and laser scatter
Counting volume: the flow of the sample stream is constant, which can be
converted to counting volume by controlling the counting duration
1
Dilution ratio refers to the Dilution ratio in the whole blood mode.
7-3
Fluidics
HGB Count
Reagents:
Diluent: used for diluting, cleansing and equal volume processing conductive environment
and cells
Measurement principle: impedance method
Counting duration: 9 s
7-4
Fluidics
7-5
Fluidics
Appearance:
2-way valve 3-way valve
Spring pole
Function:
2-way valve: to build up or cut off a passage. When power off, the passage from
the inlet of the valve to outlet is cut off; when power on, the passage is build up.
3-way valve: to switch among passages. When power off, the public end and the
NO (normally open) end are connected; when power on, the public end and the
N.O.(normally open) end are connected.
Note: the operating voltage of Mindray valves is 12V, and maximal bearable pressure
is 200KPa. The internal movement of the valves is driven by electromagnet and the
restoration is driven by the spring, so it is recommended not put the valves power-on
for too long. When the electromagnet valve is working, the spring pole will lower
down, and it will rise to the initial position when power off. You can touch the spring
pole and feel the descending or ascending, in order to determine whether it is in
action.
7-6
Fluidics
Function: Compared with regular 2-way Mindray valve, the 2-way Mindray
pressure-proof valve can endure higher reverse pressure. The operation principle of
the 2-way Mindray pressure-proof valve is the same with that of regular 2-way
Mindray valve.
Note: When replacing the valves, please note the distinction between regular 2-way
valves and pressure-proof valves. SV03 in Liquid Flow Diagram is a 2-way
pressure-proof valve.
Function: Same as the Mindray valves. Compared with 2-way Mindray valves, this
valve provides higher pressure resistance and smaller pump volume for preciser flow
control and broader range of temperature and pressure.
Note: the maximal bearable pressure of the LVM fluidic valve is 200KPa, and the CV of
the flow is about 0.03. SV11 in the fluidics diagram is a LVM fluidic valve.
7-7
Fluidics
Appearance:
Appearance:
LF
7-8
Fluidics
Appearance: N/A
Function: Composed of a large volume syringe and a small volume syringe, the syringe
linkage is driven by a motor and a linkage. The parameter and the function of the
syringe linkage are shown in the table below:
Specific
Name Function
ation
Used for quantitative aspiration, distribution
Small Full
and secondary aspiration of blood samples, and for
volume scale
injecting the sample into the flow cell for
syringe 250L
measurement
Used for quantitative addition of diluent to
Large Full WBC and RBC bath, wipe fluid supply, cleaning
volume scale of interior and exterior of sample probe and
syringe 10mL reaction bath, forcing the sheath into the flow cell,
cleaning of the flow cell and sample preparation.
7-9
Fluidics
DP1, DP2
Appearance:
Function:
DP1: 1mL volume. Used for addition of DIFF reagent
preheat Bath
Appearance:
7-10
Fluidics
Function:
Used for heating DIFF reagents to ensure the temperature of DIFF reaction.
Appearance:
Function:
LP: Used for draining the wipe, WBC bath, RBC bath and vacuum chamber,
and for creating vacuum in the vacuum chamber
7-11
Fluidics
Appearance:
Open-vial sample probe
Function: Used for providing a rigid cavity with resistance to blood sample corrosion,
which can sample and dispense the blood as well as aspirate and dispense probe
cleanser.
Note: the sample probe is flat-tipped with a side opening to ensure normal aspiration in case that the
tip touches the bottom of the sample tube.
7-12
Fluidics
Appearance:
Appearance:
Function: Used for monitoring the fluid pressure. When the pressure is obviously
abnormal or beyond the setting range, the sensor will send an alarm signal.
7-13
Fluidics
Appearance:
Function: Used for controlling the flow direction of the DIFF tube to prevent reverse
aspiration
7.5.14 Baths
WBC bath: Used for providing a place for WBC sample reactions and
supplying well reacted DIFF and BASO samples, and for HGB measurement.
RBC bath: Composed of primary bath, secondary bath and aperture. Used for
providing a place for RBC sample reactions and for RBC/PLT measurement.
Vacuum chamber: Used for creating and keeping a stable negative pressure
for RBC impedance count.
preheat bath: Used for heating DIFF reagents to ensure the temperature of
DIFF reaction.
7-14
Fluidics
Main function:
1. Aspirate and dispense samples. Aspirate 15 L of blood sample by conjunctive use of
small volume syringe of the syringe linkage (SR) and the sample probe (SPB), and
dispense the blood sample.
2. Clean the interior and exterior of the sample probe. The interior is cleaned by the
collaboration of the large volume syringe of the syringe linkage and SV03 and 11 valves.
The exterior is cleaned by the diluent which is forced by the large volume syringe
through SV08, SV07, secondary RBC bath and SV09 into the wipe, with waste fluid
recovered by probe wipe, vacuum chamber and waste pump.
3. Aspirate and dispense probe cleanser. During the aspiration, the SV03 is energized.
2mL of probe cleanser will be aspirated by the SR from the SPB and stored mainly in the
cleanser tanks (T7 and T8). During the dispensation, the SPB is transported by the
sampling assembly to the RBC bath and WBC bath, and the SV03 is kept energized. A
certain volume of probe cleanser in the SPB will be dispensed by the SR to the reaction
baths.
7-15
Fluidics
DIFF measurement
As the background solution, the diluent flows through the large volume syring, SV03, SV11,
PV18 into the WBC bath. After the blood sample is dispensed by the sample probe to the WBC
bath, the DIFF lyse is added by the electromagnetic metering pump (DP1) through the SV01 and
the preheat bath. The diluent is forced by the SR through SV06, T13 and T14 into the isolation
chamber 1, generating bubbles which mix the sample fluid in the WBC bath. The sample is then
supplied along the illustrated orange lines to the bottom end of the flow cell. A sheath flow is
generated by the large volume syringe along the illustrated blue lines, and the sample is forced
by the small volume syringe into the flow cell for measurement to obtain the differential results
of the white blood cells. After the measurement, use the sheath to clean the flow cell and the
sample probe. Force the diluent through the SV03, SV11, C11, T35, SV14 and T36 into the
isolation chamber 2 to clean the sample supply tube.
7-16
Fluidics
for measurement to obtain BASO differential result and WBC count. After the measurement, use
the sheath to clean the flow cell and the sample probe. Force the diluent through the SV03,
SV11 and PV18 into the WBC bath to clean the sample supply tube and the WBC bath. During
the cleaning process, part of the diluent flow away through C11, T35, SV14 and T36 to clean the
3-way connector C11. Finally, the WBC bath is drained by SV15, vacuum chamber (VC) and
waste pump.
HGB Count
The measurement principle of HGB channel is the colorimetric method, which obtains HGB
concentration by comparing the transmitted light intensity between background and blood. The
transmitted light intensity of the pure diluent in the WBC bath is measured at the beginning of
the count. The transmitted light intensity of the diluent with blood is measured after the BASO
reaction is completed (before preparation of the BASO sample). The HGB value can be
calculated by comparing the above two values.
7-17
Fluidics
The diluent is added by the SR along the blue line (T3, T18, SV05, T21) to the RBC
bath. The diluted blood sample is dispensed by the sample probe to the RBC bath, and
then is mixed by the bubbles generated when waste cleaning fluid (for cleaning the
DIFF sample fluid in the sample supply tube) enters the isolation chamber 2 through
SV14 and T36. The sample is aspirated by the negative pressure in the vacuum
chamber into the secondary bath (orange lines in the illustration). The cells will be
measured while passing through the aperture. The sample volume is calculated from
the count duration. After the measurement, the RBC bath is drained by the waste
pump, the vacuum chamber and the SV13. In order to clean the secondary bath, the
diluent is forced by the syringe through SV07, T51 and T52 into the secondary bath,
then through T43 and SV09 into the probe wipe, and drained by the waste pump.
7-18
Fluidics
When connecting the adapter with the Teflon tube, make sure the insertion depth is
13~15mm. Keep no clearance between the two connected rigid tubes as far as
4 possible. Keep the end of the Teflon tubes smooth and unwrinkled.
Strap the connections between any 1.0 Teflon tubes and the adapter. Strap at a
5 position near the end of the Teflon tube, and leave 3-5 mm of Telfon tube aside.
When connecting hoses with connectors, valve ports or dosing tubes on the
6 reaction bath, the end of the hose shall be fully inserted beyond the barbs.
7 It is not necessary to over tighten the straps for fastening the tubes.
For T-adapters, the side ports and the middle port shall be treated differently. Please
8 assemble in strictly accordance with the illustration.
There are strict tolerance requirements for lengths of the following tubes: T4 and
T33: 1 mm, T63: 2 mm T7, T8: 3 mm.
9 Tolerances for the other tubes: for length less than 50 mm, the tolerance is 1 mm;
for length between 50 and 400 mm, the tolerance is 2 mm; for length more than
400 mm, the tolerance is 5 mm.
When connecting or replacing tubes on the analyzer, never use any blades or other
10 sharp tools.
11 Never scald any hose with hot water.
Before or after assembly or maintenance, keep all the hoses, connectors or fluidic
components intact and in good condition without any scratches, deformation or
12 distortion.
Thick 50 tubes can not be used again after disconnected from valve ports or
13 connectors
14 Cut T75 and case it between T34 and C11
15 Assemble the check valves so that the bigger end is connected to the preheat bath
Assemble the filters in the right direction so that the words 43 m is shown from
16 top to bottom
7-19
Fluidics
0~0.9 s The small volume syringe of the SR syringe linkage aspirate 15 L of blood
sample from the sample probe
The large volume syringe aspirates 600 l of diluent from the diluent tank
0.1 s Measure the HGB blank voltage
0.3~2.8 s The liquid pump (LP) is turned on to drain the WBC bath
1~2.7 s Aspirate 5360 l of diluent with the SR (with SV03 and PV18 energized and
SV11 open to T64)
0.1~3.1s Burn the aperture on the RBC bath
7-20
Fluidics
7-21
Fluidics
7-22
Fluidics
7.5~8 s Inject the diluent through SV06 into the isolation chamber 1 to generate
bubbles which mix the sample fluid in the WBC bath
7.4~8.1 s The sample probe travels up to the wipe position and the LP draws waste
fluid from the wipe
8.1~8.4 s The interior of the sample probe is cleaned and the LP draws waste fluid
from the wipe (to 9.2 s)
8.5~9.1 s The syringe aspirates 1580 l of diluent from the diluent tank
8.8~9.6 s The sample probe travels down into the WBC bath
8.6~10.6 s DP1 aspirates the DIFF reagents
7-23
Fluidics
9.6~10.3 s The small syringe aspirates 12 l of diluted sample through the probe
from the WBC bath; the large syringe aspirates 480 l of diluent from the diluent tank
10.3~11.3 s The sample probe travels up to the wipe position; the exterior of the probe is
cleaned by the wipe; the LP draws waste fluid from the wipe
10.6~11.6 s Use DP1 to add DIFF reagent to the WBC bath
11.4~12.3 s Inject the diluent through SV06 into the isolation chamber 1 to generate
bubbles which mix the sample fluid in the WBC bath
11.2~11.9 s The sampling assembly swings to the position above the RBC bath
9.2~10.1 s ,11.4~12.1 s The RBC bath is drained.
7-24
Fluidics
12.4~13.3 s SR injects 1600 L of background solution through SV05 to the RBC bath.
12.3~13.2 s The sample probe travels down into the RBC bath
13.4~14.9 s The sample probe dispenses the blood and clean the interior with 800 L of
fluid
15.4~16.6 s The sample probe travels up to the wipe position and the LP draws waste
fluid from the wipe
7-25
Fluidics
C14 DILUENT
T25 T49
CV1
J7-T31-
T21 J29 T30
J8
SV05 C15
DP1 T50
Preheat SV01
Bath T32
T26
T24
C27
J14-T48-J15-T80-J32 T47
T17
T77
SV02 DP2
J30-T78-
T18
Case
J31
LH Reagent Bottle
T79 SV07 C18
C19
T29
T71
J9
SV04
WBC RBC
T51
PV18
J25-T23-J26
T72
T75
J22-T64-J23-T65-
J12-T45-
T54
J10-T35-
Sensor
J13
J16-T59-
J18-T60-
J11
J17
J19
C20
J24
SV12
Isolation Chamber
Isolation Chamber
C21
T3
DIFF Reagent
T61 T62
T70
1
Bottle
SV06
2
C5 SV13
T9
T40
T55
C9
T15
T36
C3 SV11
T11 T16 C7
J5-T7-J6- SV15
T41
C26
T10
Hydraulic
T53 T8
Sensor
C2 T46 C16
J4-T6 C8
Transducer
J3-T5
T2
T57 T58
T56
T42
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
T1
SPB
J1-T4-
Diluent Tank
J2
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR
7-26
Fluidics
7-27
Fluidics
C14 DILUENT
T25 T49
CV1
T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
Preheat SV01
Bath T32
T26
T24
C27
J14-T48-J15-T80-J32 T47
T17
T77
J30-T78-J31
SV02 DP2
T18
Case
T79
LH Reagent Bottle
SV07 C18
C19
T29
T71
J9
SV04
WBC RBC
T51
PV18
J25-T23-J26
C11 C10
J20-T63-J21 T34 T52
Case T33
J22-T64-J23-T65-J24
C6
J12-T45-J13
Temperature
J10-T35-J11
T72
T75
J16-T59-J17
J18-T60-J19
T54
Sensor
Isolation Chamber
Isolation Chamber
C20
SV12
C21
T3
DIFF Reagent
T61 T62
T70
Bottle
SV06
C5 SV13
T9
T40
T55
T15
T36
C3 SV11
T11 T16 C7
SV15
T41
Hydraulic C26 J5-T7-J6-T8
T10
Sensor T53
C2 T46 C16
J4-T6 C8
Transducer
J3-T5
T2
T57 T58
T56
T42
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
T1
SPB
J1-T4-J2
Diluent Tank
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR
31.7~33.6 s The large volume syringe aspirates the diluent from the diluent tank ; the
small syringe aspirates 156.3 l of sample fluid from the WBC bath
34~35.5 s Clean the sample supply tube with SV11 and SV14; clean the flow cell with
SV04 and SV16; inject the waste fluid into the isolation chamber 2 to generate bubbles for
mixing the sample in the RBC bath
31.5~39.1 s Create negative pressure in the vacuum chamber
7-28
Fluidics
C14 DILUENT
T25 T49
CV1
J7-T31-
T21 J29 T30
J8
SV05 C15
DP1 T50
Preheat SV01
Bath T32
C27
J14-T48-J15-T80-J32 T47
SV02 DP2
Case
T79 SV07 C18
C19
SV04
WBC RBC
PV18
J25-T23-J26
C20
SV12
C21
SV16 SV14 C12 C13
T61 T62
SV06
C5 SV13
C4 T12 T13 T14 T37 T38
T39
C9
T36
C3 SV11
T11 T16 C7
J5-T7-J6- SV15
Hydraulic C26
T53 T8
Sensor
C2 T46 C16
J4-T6 C8
Transducer
T57 T58
SV10 T67
C1 SV03
T43 T66
SPB
VC
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR
35.8~37.1 s Aspirate 800 L of BASO sample fluid from the WBC bath into the sample
supply tube (T63 and T65)
37.4~51.2 s The large syringe injects the diluent through SV04 into the flow cell to form
the sheath
37.4~37.6s SV03 ON; the sample in T65 enters the flow cell quickly by the help of the
large syringe
37.6~51.2 s SV03 OFF; the small syringe injects the sample from the supply tube into the
flow cell to form a stable sample stream
39.6~51.1 s Perform BASO measurement and WBC count
35 s The constant current source of RBC bath turns ON
35 s~49 s SV12 valve ON; the fluid in the primary RBC bath is forced by the vacuum
through the aperture into the secondary bath
39.8~48.8 s Perform RBC and PLT counts
7-29
Fluidics
51.3~52 s PV18 stays ON; the sample supply tube and the 3-way connector C11 is
cleaned by SV03, SV11, and SV14 Clean the flow cell and the sample probe by SV04
52.1~53.8 s The syringe aspirates the fluid
51.1~52.9 s The RBC bath is drained
7-30
Fluidics
C14 DILUENT
T25 T49
CV1
T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
Preheat SV01
Bath T32
T26
T24
C27
J14-T48-J15-T80-J32 T47
T17
T77
J30-T78-J31
SV02 DP2
T18
Case
LH Reagent Bottle
T79 SV07 C18
C19
T29
T71
J9
SV04
WBC RBC
T51
PV18
J25-T23-J26
C11 C10
J20-T63-J21 T34 T52
Case T33 C6
J22-T64-J23-T65-J24
J12-T45-J13
Temperature
J10-T35-J11
T72
T75
J16-T59-J17
J18-T60-J19
T54
Sensor
C20
Isolation Chamber
Isolation Chamber
SV12
C21
T3
DIFF Reagent
C13
T61 T62
T70
Bottle
SV06
C5 SV13
T9
T40
T55
T15
T36
C3 SV11
T11 T16 C7
SV15
T41
Hydraulic C26 J5-T7-J6-T8
T10
Sensor T53
C2 T46 C16
J4-T6 C8
Transducer
J3-T5
T2
T57 T58
T56
T42
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
T1
SPB
J1-T4-J2
Diluent Tank
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR
7-31
Fluidics
7-32
Fluidics
7-33
Fluidics
T26
T24
T17
T77
J30-T78-J31
T18
LH Reagent Bottle
T29
T71
J9
T51
J22-T64-J23-T65-J24
J12-T45-J13
J10-T35-J11
T72
T54
J16-T59-J17
J18-T60-J19
Isolation Chamber
Isolation Chamber
T3
DIFF Reagent
1
T70
Bottle
T9
T40
T55
T15
T41
T10
J3-T5
T56
T42
J27-T22-J28
Diluent Tank
J1-T4-J2
T73
T74
Startup cleaning
The fluidic actions on startup can be divided into three procedures:
1. Initialization of fluidics components: initialization of sampling assembly and syringe
assembly; 1 metering pump action (without consumption of reagents); creating vacuum and
releasing vacuum.
2. Cleanup: including cleaning of all the tubes and components on the analyzer; removing
bubbles from the flow cell; discarding 1 ml of DIFF reagent and 0.2 ml of LH reagent.
3. Background: WB-CBC+DIFF measurement
If the background fails, perform the cleanup again and measure the background.
For startup after an abnormal shutdown, perform the cleanup twice.
Standby
The instrument will enter standby status after idling for 15 to 30 minutes (defaulted as 15
minutes). After entering standby status, operations without fluidic actions can be performed
from the screen.
Exit standby status 1: Standby for less than 1 hour.
Clean the exterior of the sample probe and the WBC bath and rebuild the
7-34
Fluidics
7-35
8 Hardware System
POWER Supervisor
supervise system Indicator
POWER board
State
supervise
The hardware system consists of five modules, including power supply, data stream channel,
control system, drive components and peripherals. The functions of each modules are as follows:
1. Power supply: provides all kinds of power for each board, component and devices in the
hardware system;
2. Data stream channel: for extraction, modulation, amplification, collection and preprocessing
of signals;
3. Control system: for data collection, data processing, result display and sample storage. The
8-1
Hardware System
control system is also the scheduling and managing center, which controls and responds to
all the peripherals and devices;
4. Drive/monitor: for controlling valves, pumps and motors, monitoring photocoupler and other
important parameters, collecting measurement data and triggering alarms;
5. Peripheral interface: includes display/touchscreen, USB ports (for printer, keyboard and
barcode scanner) and Ethernet interface. Peripherals also includes operation indicators and
key inputs.
8-2
Hardware System
(such as failure to power up or immediate system self-protection after power-up), it will be necessary to
start troubleshooting from the system level. Figure 8-3 shown the flowchart for power anomaly check.
Figure 8-4 shows the filter which is located below the rear of the analyzer, used for controlling power
supply and frequency filtering.
8-3
Hardware System
8.2.1 Overview
The digital control board consists mainly of a digital part and an analog part. The analog part
implements mainly the A/D conversion of analog signals into digital signals, such as optical channel
and monitoring voltage. As the essential part of the digital control board and the whole hardware
system, the digital part implements data processing, output, control and communication.
Section 8.2 of this manual is the guideline for service and troubleshooting of the control board.
8.2.2 Components
The block diagram of the control board is shown in Figure 8-6. The control board consists mainly
of digital circuit, plus part of the ADC circuit which implements the A/D conversion. The digital circuit
module implements data processing, result saving and output. As the essential part of the digital control
board and the whole hardware system, the digital circuit is also responsible for control and
communication. The ADC circuit is mainly responsible for digitizing WBC, RBC/PLT, optical signals
and all kinds of analog monitoring signals with an A/D converter.
The control part of the control board employs the CPU+FPGA structure to achieve the
following main functions:
A/D conversion
Data processing
Peripheral interface implementation
Control interface extension
8-4
Hardware System
Description
A/D conversion
Converts the analog signals to digital signals which can be processed by FPGA or CPU.
Data processing
The digital signals obtained by FPGA from A/D samples are digitally filtered to save
the particle parameters. The data then is transmitted to CPU by interruption or other methods
for further processing. After processing, the results will be shown on the LCD.
8-5
Hardware System
J86 supervise
signal interaface
J1 Network
J85 Optical
signal input
interface
8-6
Hardware System
8-7
Hardware System
PIN9: GND
J81 Analog board and / Analog board and
control board SPI control board SPI
interface communication
interface
J85 Optical signal PIN1: FS Signal Interface for optical
input interface PIN3: SS Signal signal inputs
PIN5: SF Signal amplified by the
PIN7: WBC Signal analog board
PIN9: RBC Signal
PIN11: 5V
PIN12: 5V
PIN13: GND
PIN14: GND
J86 Analog board PIN13: 5V Provides supervisory
supervisory signal PIN20: GND signals for the
interface control board
J99 Aspirate key PIN2: GND Aspirate key switch
switch interface interface
8-8
Hardware System
Test
No. Description Zone Diagnosis
Point
1 TP1 VDD18 1.8 V Zone 6 The voltage is not 1.8V: control board failure
voltage monitoring or power board failure
point
2 TP2 VDD 3.3V voltage Zone 6 The voltage is not 3.3V: control board failure
monitoring point or power board failure
3 TP3 VDD12 1.2 V Zone 6 The voltage is not 1.2V: control board failure
voltage monitoring or power board failure
point
4 TP4 Backlight enable Zone 5 The voltage is not 3.3V: control board failure
signal
5 TP5 Backlight PWM Zone 5 The voltage is not 0.7~1.2V: control board
signal failure
6 TP6 Backlight GND Zone 5 /
signal
8 TP9 Analog ground Zone 1 /
9 TP10 ADC U79 SPI Zone 1 /
clock signal
10 TP11 ADC U78 SPI Zone 1 /
clock signal
11 TP12 ADC U79 2.5V Zone 1 The voltage is not 2.5V: control board failure
reference voltage or connection failure between control board
J86 and analog drive board
12 TP13 ADC U78 2.5V Zone 1 The voltage is not 2.5V: control board failure
8-9
Hardware System
8-10
Hardware System
35 TP36 5 V voltage Zone 6 The voltage is not 5V: control board failure or
monitoring point power board failure
36 TP37 12V voltage Zone 8 The voltage is not 12V: control board failure
monitoring point or power board failure (the ground connection
of this voltage is J68_PIN2 instead of GND)
37 TP44 DDR2 0.9V Zone 6 The voltage is not 0.9V: control board failure
voltage monitoring or power board failure
point
38 TP45 DDR2 0.9V Zone 6 The voltage is not 0.9V: control board failure
reference voltage or power board failure
monitoring point
39 TP38, Digital ground Zone 6 /
TP39,
TP40,
TP41,
TP42,
TP43,
TP46,
TP47
40 TP48 3.3 V voltage Zone 5 The voltage is not 3.3V: control board failure
monitoring point or power board failure
8.2.4 Troubleshooting
Table 8-4 lists common symptoms and relative corrections for the control board only from the
hardware side, not including symptoms caused by software. However, many problems will need to be
tested by software.
Before troubleshooting problems related to the control board, perform the following checks:
1. whether there is any loose connecting wire or unreliable connection on the control board;
2. whether the bit numbers on the wires are matching the bit numbers on the control board
sockets; whether there is any broken or damaged wire;
3. whether the input power of board socket J68 is working properly (measured with a
multimeter, the voltage between PIN1 and PIN3 shall be 5V, and the voltage between PIN2
and PIN4 shall be 12V);
4. Verify if the indicator on the data board is normal according to Table 8-2.
After the wire connections, input power and indicators are verified to be normal, troubleshoot the
problem in accordance with Table 8-4.
8-11
Hardware System
8-12
Hardware System
8-13
Hardware System
6 Clock 1. Turn off the power and measure the Replace the
reset at voltage between the two end of Battery clip B1 button battery
every with a multimeter with the battery in place. If the
startup measured value is less than 1.8V, then the
problem is caused by a low battery. Otherwise,
proceed to the next step.
2. Measure the voltage of pin 1 or pin 4 at Replace the
X10 with a multimeter. If the measured value is control board
0V, then the problem is caused by crystal
resonator X10.
7 No 1 Check if the connecting wire of the
response aspirate key is loose or broken. If so, reconnect or
when replace the wire.
pressing the 2. If step 1 does not solve the problem,
aspirate key remove the aspirate key switch plate to check if
there is fluid inside. If so, clean the fluid and
reinstall the switch plate.
8-14
Hardware System
8.3.1 Overview
The functions of analog drive board includes measuring and amplifying the signals from the RBC
channel, the HGB channel and the optical channel, and outputting them to external boards; responding
commands of the control board; controlling mechanical components (such as motor drive assembly)
and fluidics components (such as valves and pumps); detecting component position when controlling
mechanical components (through the photocoupler); detecting pressure when controlling fluidics
components (such as pumps); reporting necessary information (temperature, voltage, etc.) to the control
board.
8.3.2 Components
The analog drive board can be divided by modules into four modules: power module, master
control module, detection module and power driving module. The modules are shown in Figure 8-9.
1 2 3 4
Motor Driver
5
7
11
RBC
12
For Debug
Air pressure
10 8 6 13
sensor
9
Signal Supervise Communication Master
HGB
preprocess signal Module Control
8-15
Hardware System
1. The power supplies power for all the electronic devices on the analog drive board, including
both input power from the analog drive board, and the power converted by the power chip;
2. The master control module is responsible for communicating with the control board and task
scheduling;
3. The detection module is responsible for collecting information such as temperature, pressure,
voltage and fluid in the tubes;
4. The power driving module is responsible for driving the power components such as motor,
heater, fan, valves and pumps;
5. The RBC detection circuit provides the RBC module with constant current source and functions
such like RBC signal modulation;
6. Provides functions such like modulation of optical signals and HGB signals.
8-16
Hardware System
8-17
Hardware System
electrode A
PIN3: AGND
J8 Analog power PIN1: AC120_A Supply analog
interface PIN2: AC120_B power for the boards
PIN4: Analog 12V
PIN5: Analog -12V
PIN3: AGND
PIN6: AGND
J9 Optical system PIN3: FS input Optical board
signal input and PIN7: SS input analog signal output
control output PIN11: SF input and control signal
PIN15: Laser current input
output
PIN18: Laser control
PIN16: AGND
J10 Temperature sensor / 3-way temperature
interface sensor interface
J11 Hydraulic sensor / Hydraulic sensor
interface interface
J12 Photocoupler / Photocoupler
interface for interface for
sampling assembly sampling assembly
J13 Photocoupler / Photocoupler
interface for interface for syringe
syringe assembly assembly
J14 Valve 13-18 driver / Drives valve 13-18
J15 Valve 1-12 driver / Drives valve 1-12
J17, J18, Fan interface / Fan interface
J19
J20 Waste pump / Waste pump
interface interface
J21 Heater interface / Heater interface
J22 Power interface for PIN1: Power 12V Digital power
digital part of the PIN3: Power 5V interface
analog board PIN5: Power 24V
8-18
Hardware System
PIN2: GND
J23 Motor driver / Motor driver
interface interface
J24 Motor driver / Motor driver
interface interface
J31 Controls analog / Controls analog
drive board DPM drive board DPM by
by the control the control board and
board and other other preserved
preserved interfaces
interfaces
8-19
Hardware System
Test Diagnosis
Description Zone
Point
TP19 Analog 12V If the voltage is not 2.28V, then the
voltage AD Zone 8 problem may be caused by power failure or
monitoring point analog drive board failure
TP21 FS baseline /
voltage AD Zone 8
monitoring point
TP22 Analog -12V If the voltage is not 2.82V, then the
voltage AD Zone 8 problem may be a power failure or analog
monitoring point drive board failure
TP23 56V voltage If the voltage is not between 1.25~1.5V,
monitoring point Zone 8 then the problem may be a power failure or
analog drive board failure
TP26 Hydraulic sensor /
Zone 3
output voltage
TP29 Pressure sensor If the voltage is not 2.5V, then the problem
2.5V voltage Zone 10 may be a power failure or analog drive
monitoring point board failure
TP30 Pressure sensor /
output voltage Zone 12
monitoring point
TP39, Motor lock Zone 5 /
8-20
Hardware System
TP40, voltage
TP49,
TP58
TP59 Reset chip Above Low signal indicates the chip is in reset
output signal Zone 7 status
TP68 Digital ground Zone 6 /
TP43, Motor pulse /
TP44, output Zone 5
TP51 monitoring point
TP69 D115 signal /
Zone 7
monitoring point
TP86 Digital 1.2V If the voltage is not 1.2V, then the problem
power Zone 6 may be a power failure or analog drive
monitoring point board failure
TP87 Digital 2.5V If the voltage is not 2.5V, then the problem
power Zone 13 may be a power failure or analog drive
monitoring point board failure
TP88 Digital 3.3V If the voltage is not 3.3V, then the problem
Right to
power may be a power failure or analog drive
Zone 11
monitoring point board failure
TP89 Digital 12V If the voltage is not 12V, then the problem
Right to
power may be a power failure or analog drive
Zone 4
monitoring point board failure
TP90 Digital 24V If the voltage is not 24V, then the problem
Right to
power may be a power failure or analog drive
Zone 4
monitoring point board failure
TP91 Digital 5V If the voltage is not 5V, then the problem
power Zone 5 may be a power failure or analog drive
monitoring point board failure
TP92 AD U41 /
conversion chip
Zone 3
voltage
monitoring point
TP112 HGB analog /
Zone 9
voltage output
8-21
Hardware System
8-22
Hardware System
8.3.4 Troubleshooting
1) Validation before troubleshooting
Before troubleshooting driver related problems, check whether there is any loose connecting wire
or unreliable connection on the analog drive board, and whether the bit numbers on the wires are
matching the bit numbers on the analog drive board sockets; whether there is any broken or damaged
wire.
When analog drive board failure is suspected, verify whether the indicators on the analog board
are normal in accordance with the table below.
After all the indicators is verified to be normal, verify the MCU and FPGA from the software
interface (click Menu click Status Version information). If all the versions are correct,
troubleshoot the problem according to the problem types listed in (2).
2) Troubleshooting
Initially locate the problem by the alarm indication, then troubleshoot the problem according to
the type as shown in Table 6-7.
Table 8-11 Troubleshooting and solutions
8-23
Hardware System
8-24
Hardware System
8-25
Hardware System
8.4.1 Overview
The power board provides the analyzer with 6 reliable power outputs, including D5V, A+12V,
A-12V, AC120V and P24V.
Interface definition
There are 6 interfaces for external connections on the power board. 4 interfaces are in socket form,
namely J1, J2, J3 and J4; the AC input wire L and N are led from the board side to the plug for external
connection; the small inverter board is directly connected to the power board by the interface socket.
The locations of each interface on the board are shown below:
PIN Definition
L Connected to utility live wire
N Connected to utility neutral wire
8-26
Hardware System
PIN3: 24V
A+12V, A-12V J3 PIN1, PIN4: GND
PIN2, PIN3, PIN5: -12V
PIN6: 12V
AC120V J4 AC
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the screws are installed on the board;
2. Connect the power cable and turn on the AC control switch. Now the analyzer will be
initialized, and all the board indicators will be illuminated.
Make sure the power assembly is firmly connected by screws with the main unit.
Only perform these removal procedures after the power board and the surroundings
are cooled down.
8.4.3 Troubleshooting
The troubleshooting procedures for the power board are shown below.
8-27
Hardware System
Figure 8-13 shows the schematic diagram of the optical path. The laser is a semiconductor laser
with wavelength of 670 nm. The laser irradiates the cells in the flow cell, which generate scattered light.
This scattered light intensity is measured by photodiode (PD) from various angles. The measurement
signal is then modulated, amplified, and transmitted to analog drive board for further processing. The
scatter measured by the photodiode can be divided into three angles: low angle scatter, medium angle
scatter and high angle scatter. The type and size of the cell can then be determined by the distribution
of scatter intensity on these angles.
8-28
Hardware System
Figure 8-14 shows the electrical connection diagram of the optical system. The analog
drive board controls the laser. When the PDs detect the light signal, they will transmit current
signals through the signal wire to the preamplifier. After I/V conversion, modulation and
amplification, the signals will be transmitted through the signal wire to the analog drive board for
further processing. A protective microswitch is connected between the analog drive board and
the laser analog board. When the optical system housing is opened, the microswitch will be
turned off, and the laser will stop working. Therefore, in order to test the laser, press the
microswitch manually to connect the electrical circuit (make sure the wire is connected to the C
pin and ON pin of the microswitch).
Preamplifier
Overview
The preamplifier is mainly used for photoelectric conversion and amplification of the three
scatters from the flow cell (forward scatter (FS, also called low angle signal), side medium angle
scatter (SS, also called medium angle signal) and side high angle scatter (SF, also called large angle
signal)). The FS/SS preamplifier and the SF preamplifier share a common PCB, and achieve the
required amplification through welded resistors.
Note: LAS and FS refer to low angle signal, MAS and SS refer to medium angle signal, and WAS
and SF refer to large angle signal, unless otherwise noted. Corresponding abbreviations refer to the
same objects.
8-29
Hardware System
Function
The function of the preamplifier includes power modulation, I/V conversion and signal
modulation.
Power modulation: filters the -12V power supplied by the analog board. The filtered ripples
will be less than 50 mV.
Signal modulation: converts the current signals to voltage signals by I/V. The signals are then
sent to the amplification unit and processed to meet the input requirements of the analog drive
board.
Function
The functions of the laser control board include power modulation, laser driving current
monitoring and laser power control.
Power modulation: filters the 12V power supplied by the analog board. The filtered ripples
will be less than 100mV.
Laser driving current monitoring: measures the electrical current of the laser and send the
results to the analog board for monitoring.
Laser power control: the laser is controlled by a constant power control method. The laser
output power is monitored in real time by a photodetector inside the laser. The result forms a
loop system by negative feedback to achieve constant output power. The power is controlled
within the range of 3 mW~5 mW by adjusting the potentiometer VR1 on the board.
8-30
Hardware System
Adjusting laser driving current: when a laser overcurrent problem is reported, it is usually
necessary to adjust the adjustable potentiometer VR1 on the laser analog board to adjust the laser
current. when adjusting, observe the laser current changes in the Voltage and Current screen in the
Analyzer Status screen. If the current does not change and is not zero when VR1 is being adjusted, then
either the laser analog board or the laser fails. Otherwise the problem may be caused by wire failure,
and it is required to check the wires before adjustment. It is also necessary to adjust the current after
replacing the laser analog board to keep the current within the acceptance. The adjusting method is the
same with above.
8.5.3 Troubleshooting
Table 8-14 Troubleshooting and solutions of optical problems
8-31
Hardware System
8-32
Hardware System
8.6.1 Overview
The touchscreen drive board provides the interface between the touchscreen and the control board,
converting the user's touch operation to identifiable signals for the control board. The touchscreen drive
board needs to be adjusted before use.
8.6.2 Components
The core of this board is a 4-wire touchscreen control chip TSC2004IRTJR, which communicates
with the control board through the I2C interface to transmit the position information of the touch
points.
8.6.3 Troubleshooting
Table 8-15 Troubleshooting and solutions of the touchscreen
8-33
Hardware System
8.7.1 Overview
The indicator board provides the user with sound or light indications to inform the current
analyzer status.
8.7.2 Components
The indicator board consists of red, yellow and green indicators, control circuits and buzzer drive
circuit. The indicator board does not need to be adjusted before use.
8-34
Hardware System
8.7.3 Troubleshooting
Table 8-16 Troubleshooting and solutions of the indicator board
8.8.1 Overview
The motor is used for driving assemblies such like sampling assembly and syringe assembly. The
photocoupler is used for detecting the motor position. The microswitch is used for starting the blood
test process. The schematic diagram of the motor and the photocoupler is shown below.
8-35
Hardware System
8.8.2 Troubleshooting
Table 8-17 Troubleshooting and solutions of motor, photocoupler and microswitch
the specified 2 Check there is any loose or broken wire between the
photocoupler and the analog drive board. If there is,
position
replace the corresponding wire.
2 Photocouple The motor can
3. If there is no problem in the wires, replace the
r not move to
photocoupler.
certain position
8-36
Hardware System
8.9.1 Overview
The liquid sensor board is used to detect whether there is liquid in the tube, judge whether
there is liquid or not by the refractive ratio for the refractive ratio is not the same when there is
liquid or not.
8.9.2 Composition
The main part of the board is photocoupler, there is a LED indicator at the back of the board,
if there is not barrier between the photocoupler transmitting tube and receiving tube, the LED
light is off; if the photocoupler is blocked, the LED light is on. When testing the board function,
use the card to perform photocoupler barrier testing.
8.9.3 Troubleshooting
When testing the reagent, if there is reagent, the board TP4 output is low electrical level
(lower than 0.8V) and the LED light is off, if there is no reagent, output the high electrical level
(higher than 2V) and the LED light is on, when the error occurs, troubleshoot by the LED light
status and whether there is reagent at the testing point.
8-37
9 Heating System
Structure diagram
1. Temperature detection assembly
Symbol:
9-1
Heating System
Appearance:
Function: a. Determine if the diluent temperature is within [10C, 40C]. If not, the analyzer
will stop working and beep; b. provides the diluent temperature to calculate the bath temperature.
Appearance:
Function: perform and control heating of diluent, and detect the bath temperature after heating.
9-2
Heating System
J10
J17
Analog Drive J18 Fan System
Board J19
Function:
Power assembly: continuously heats the optical system;
Optical System: detects the temperature and determine whether to work;
Fan assembly: cools the optical system. Starts when the optical system temperature is
higher than 30, and stops when the optical system temperature is lower than 25.
9-3
10 Mechanical System
10.1. Mechanical System Overview
This section lists the locations of major analyzer components for the service personnel to
remove and replace the components. The diagrams in this manual is based on BC-5150.
BC-5000 and BC-5150 are basically the same.
Front view
10-1
Mechanical System
Back view
10-2
Mechanical System
10-3
Mechanical System
Left view
10-4
Mechanical System
10-5
Mechanical System
Left view
Figure 10-7 Right view of the main unit (right door open)
10.2. Components
10.2.1 Introduction
This section provides exploded view of the analyzer and material IDs for the service
personnel to understand the relationship between the components when removing and
replacing the components.
Note
All the material IDs listed in the Material ID column of all the parts lists are only used for the
service personnel to search the corresponding spare part ID. Please provide the spare part ID when
purchasing a spare part.
10-6
Mechanical System
10.2.2 Overall
Exploded view
Parts list
No. Material ID Material Description Spare Part ID Comments
2 Right Door / /
7 Main Unit / /
10-7
Mechanical System
10-8
Mechanical System
10-9
Mechanical System
Parts list
No. Material ID Material Description Spare Part ID Comments
1 / Mainframe / /
See Section
2 / syringe assembly /
11.2.6
3 115-014601-00 1ml Pump(eject) 115-014601-00 /
22 / right door / /
10-10
Mechanical System
piece
30 115-016034-00 DIFF Lyse Cap Assembly 115-016034-00 /
31 / Reagent bottle,500ml / /
32 / Reagent bottle,220ml / /
10-11
Mechanical System
Parts list
No. Material ID Material Description Spare Part ID Comments
4 / screen module(BC-5150) /
9 / shield(5150) / /
10-12
Mechanical System
Parts list
No. Material ID Material Description Spare Part ID Comments
4 / screen module(BC-5000) /
5 / The M3X8 combination Bolt /
9 / Shield(5000) / /
Cross pinhead screw with washer
10 / / /
M3X10
10-13
Mechanical System
Parts list
No. Material ID Material Description Spare Part ID Comments
10-14
Mechanical System
Parts list
No. Material ID Material Description Spare Part ID Comments
10-15
Mechanical System
Parts list
No. Material ID Material Description Spare Part ID Comments
10-16
Mechanical System
Parts list
No. Material ID Material Description Spare Part ID Comments
10-17
Mechanical System
Parts list
No. Material ID Material Description Spare Part ID Comments
10-18
Mechanical System
Parts list
No. Material ID Material Description Spare Part ID Comments
10-19
Mechanical System
Parts list
No. Material ID Material Description Spare Part ID Comments
10-20
Mechanical System
Parts list
No. Material ID Material Description Spare Part ID Comments
10.3.1 Tools
The following tools may be needed during removal and replacement of components:
Crosshead screwdriver (107)
Flathead screwdriver
Tweezers
Pliers
Cutting pliers
Hex wrench set
10-21
Mechanical System
Stop the blood tests. Adjust the sample probe to the horizontal sampling position. Shut
down the analyzer and disconnect all the connections with accessories and peripherals.
Disconnect the external power supply.
All the analyzer components and surfaces are potentially infectious. Take proper
protective measures for operation and maintenance.
The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective equipment (e.g.
gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory.
If the reagents accidentally spill on your skin, wash them off with plenty of water and if necessary, go see a
doctor; if the reagents accidentally spill into your eyes, wash them off with plenty of water and
immediately go see a doctor.
Please eliminate static electricity before disassembly. While removing the components with electrostatic
sensitive mark, please wear protective equipment such like an antistatic wrist strap or antistatic gloves
to avoid ESD damage to the components.
During reassembly, please connect the wires correctly and keep them in proper positions to avoid short
circuit caused by damaged wires.
Use screws of suitable models during reinstallation. Using wrong screws may result in equipment damage.
Furthermore, during usage after reinstallation, a wrong screw may become loose and fall off, resulting
in unexpected product damage or personal injury.
Please disassemble the equipment in the correct order. Failure to do so may result in irreversible damage
to the equipment.
Please make sure all connections have been disconnected before disassembling the components. Be careful
not to break the wires or the connectors during disassembly.
Please store the removed screws and other parts in separate places for reinstallation purpose. Be careful
not to drop, contaminate or lose these parts.
During disassembly, separate the materials by module to avoid misusing or missing materials during
reassembly.
During reassembly, please assemble first the components then the main unit. Be careful with the wire
connections. Place the wires in proper position.
During the disassembly, make sure the site is smooth without foreign materials to avoid screen
scratches.
10-22
Mechanical System
All operations must be done by professionals. Insulating gloves must be worn when servicing.
After assembly, check all the fluidic tubes. Folding is strictly prohibited.
3. Remove all the cables from the analog drive board as shown below. Unscrew the 8
M38 combination screws and remove the analog drive board.
10-23
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check all the connections on the analog drive board and make sure there is
no mistakes.
2. Start the analyzer and power on the analog drive board. Check if the analog drive
board power and indicators are working properly.
3. Perform blank background count. If no alarm occurs, then the analog drive board is
successfully replaced. Otherwise troubleshooting is needed.
3. As shown below, unscrew the 2 M38 combination screws and remove the USB ground
plate and conductive foam as a unit.
10-24
Mechanical System
10-25
Mechanical System
During installation, verify that the ground wire is connected to the correct ground pin properly.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct.
3. After startup, press the button from the system menu, select
MaintenanceSelf-testFan Self-testclick Spin. When the Spin column
displays spin, check if the fans on the both sides of the back plate power switch can spin
properly. If so, click the Stop button and check if the fan is stopped when the Stop
column displays stop.
10-26
Mechanical System
4. As shown below, rotate the RBC back pool 90 counterclockwise and remove the RBC
back pool. Use tweezers to remove the front bath washer and the Ruby Red Cell Counter(D50um).
10-27
Mechanical System
5. As shown below, rotate the secondary RBC bath 90 counterclockwise and remove the
secondary RBC bath. Use tweezers to remove the primary bath washer and the microsensor.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. Verify normal operations by starting the analyzer.
Before removing the reaction bath shield box, please adjust the sample probe position so that the
sample probe leaves the shield box. Otherwise the sample probe may bend or hurt the operator.
Install the Ruby Red Cell Counter(D50um) so that the concave of the sensor is facing the center of
the RBC count pool.
pool
10-28
Mechanical System
2. As shown below, cut off the straps which fasten the tube between the reaction bath to the
isolation chamber filter. Rotate the secondary RBC bath 90 counterclockwise and remove the
secondary RBC bath.
3. As shown below, unscrew the 2 M3 screws which fasten the RBC count pool module.
Remove the RBC count pool module. Use tweezers to remove the ront bath washer and the Ruby
Red Cell Counter(D50um).
Note: Use angled tweezers to push the Ruby Red Cell Counter(D50um) out from the RBC
count pool center
Before removing the reaction bath shield box, please adjust the sample probe position so that the
sample probe leaves the shield box. Otherwise the sample probe may bend or hurt the operator.
Install the Ruby Red Cell Counter(D50um) so that the concave of the sensor is facing the center of
the RBC count pool.
pool
10-29
Mechanical System
3. Follow the steps in Section 10.4.1 to remove the back plate, and disconnect the HGB unit
connector.
4. As shown below, unscrew the 2 M3 screws which fasten the pool cover. Remove the lower
part of the HGB count pool module. Rotate the bath counterclockwise 90 and remove the HGB
amp module.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. Verify normal operations by starting the analyzer.
Before removing the WBC heat preservation box,, please adjust the sample probe position so that
the sample probe leaves the shield box. Otherwise the sample probe may bend or hurt the
operator.
10-30
Mechanical System
2. Unscrew the M38 compound screw, remove the liquid sensor board installation kit and
PCBA assembly of the liquid sensor board.
3. Remove the pipe and wires of the liquid sensor board and cancel the liquid sensor board
PCBA.
Note: During the installation, connect the PCBA connector at the square hole to the DIFF
reagent pipe, connect the PCBA connector at the round aperture to the LH reagent pipe.
10-31
Mechanical System
1.Perform step1and step 2 of section 10.4.10, take off the liquid sensor board installation kit
and PCBA assembly of the liquid sensor board.
2.Unscrew the 3 M38 combination screws and remove the reagent bottle plate cosmetic
piece.
10-32
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct;
3. After startup, press the button from the system menu, select
MaintenanceTemp.& Press. Calibration and check if the preheat bath temperature
column is displayed in red.
Note: If the wire head retracts back from the hole, please follow the steps in Section 11.4.10
to remove the reagent bottle baffle.
Before removing the WBC shield box, please adjust the sample probe position so that the sample
probe leaves the shield box. Otherwise the sample probe may bend or hurt the operator.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct;
3. After startup, press the button from the system menu, select
10-33
Mechanical System
Before removing the WBC shield box, please adjust the sample probe position so that the sample
probe leaves the shield box. Otherwise the sample probe may bend or hurt the operator.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. After startup, press the button from the system menu, select
maintenanceSelf-testValve Self-test and click on the number (which is printed on the
fluidics separator) to see if the corresponding valve is working correctly.
Note: If the wire head retracts back from the hole, please follow the steps in Section 11.4.10
10-34
Mechanical System
4. As shown below, unscrew the 4 M38 combination screws and remove the pump.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
10-35
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. After startup, press the button from the system menu, select
10-36
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Verify the tube connections and the electrical connections are correct.
2. After startup, press the button from the system menu, select
StatusTemp.& Pressure and check if the Diluent temperature column is displayed
in red.
10-37
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. After startup, press the button from the system menu, select
MaintenanceSample Probe Debug to verify the three posiaons of the sample probe
(Initial position, Up"," Middle position and Down position of RBC bath and WBC
bath) and ensure the reliable operation of the sample probe.
4. Verify normal operations by starting the analyzer.
Assembly
1. Remove the sample probe assembly in accordance with Section 10.4.19.
2. As shown below, first remove 2 set screws, then remove the 4 M310 screws, the Lower
positioning sleeve and the motor.
10-38
Mechanical System
Assembly
1. Remove the sample probe assembly in accordance with Section 10.4.18.
2. As shown below, unscrew the 1 M34 screw and remove the Transducer discreteness.
3. As shown below, first remove 2 set screws from the fixing hole, then remove the 4 M310
screws, the Upper positioning sleeve and the motor.
10-39
Mechanical System
2. Follow step 1 and 2 in Section 10.4.7 to remove the right panel and the RBC shield box.
3. As shown below, remove the probe wipe clamp and the Probe wipe. Disconnect the tube from
the Probe wipe.
Installation:
Reverse the removal procedure.
10-40
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Verify the tube connection is correct.
2. After startup, press the button from the system menu, select
MaintenanceSample Probe Debug to verify the three positions of the sample probe
(Initial position, Up"," Middle position and Down position of RBC bath and WBC
bath) and ensure the reliable operation of the sample probe.
10-41
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct;
3. After startup, press the button from the system menu, select
MaintenanceSample Probe DebugIniaal posiaon so that the sample probe goes
back into the initial position. Then click on RBC bath and WBC bath respectively to
verify normal operations of the sample probe.
4. Verify normal operations by starting the analyzer.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct;
3. After startup, press the button from the system menu, select
10-42
Mechanical System
5. Follow the steps in Section 10.4.1 to remove the back plate, and disconnect the cable
connectors of the Panel Module from the Pinaster board.
6. Follow the steps in Section 10.4.10 to remove the reagent bottle baffle. Move the front
cover signal wire from the back to the front and remove it.
Note: During installation, the excess part of the signal wire shall be completely inserted into
the analyzer. Failure to do so will result in signal interference.
10-43
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;
3. Start the analyzer and verify the indicators can be illuminated.
3. As shown below, remove the cables from the touchscreen. Unscrew the 2 M38
combination screws and remove 6301 touch control board PCBA.
10-44
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;
3. Start the analyzer and verify normal operations of the touchscreen.
Verification: 1. Check if each connecting wire between the LCD Module and the Pinaster
board is locked.
2. Check if all the components are installed and fastened in position.
3. Calibrate the touchscreen.
4. Start the analyzer and verify normal operations of the touchscreen.
10-45
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;
3. Start the analyzer and verify that the screen is working correctly.
10-46
Mechanical System
4. As shown below, unscrew the 2 M38 combination screws and remove the microswitch
from the small hole in the front panel.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;
3. The Start key can be pressed down and released normally with an audible click.
4. Verify normal operations by starting the analyzer.
10-47
Mechanical System
3. As shown below, unscrew the 4 M312 screws and flat washers. Remove the syringe
assembly and remove the cables from the syringe.
4. As shown below, unscrew the 3 M38 combination screws and special bolt 3, then remove
the syringe.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections and the tube connections are correct;
3. Verify normal operations by starting the analyzer.
10-48
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections and the tube connections are correct;
3. Verify normal operations by starting the analyzer.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections and the tube connections are correct;
3. Verify normal operations by starting the analyzer.
10-49
Mechanical System
2. Remove the tubes from the hydraulic detection assembly, and disconnect the cable
connections from the top.
3. As shown below, unscrew the 2 M38 combination screws and remove the liquid detect
assembly.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. Verify normal operations by starting the analyzer.
Front Panel
1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table.
2. Remove the tubes from the assembly.
3. As shown below, unscrew the 2 M3x8 combination screws and remove the electromagnetic
pump/valve. Pull the connector out from the hole. Disconnect the cable connectors (leave the
connectors at the left side for installation purpose).
10-50
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. Verify normal operations by starting the analyzer.
Note: When installing the left door assembly, the hook at the bottom of the left door shall
engage the bottom plate so that the access door in the left door will not swing.
10-51
Mechanical System
1. Follow step 1 and 2 in Section 10.4.7 to remove the right panel and the RBC/WBC shield
box.
2. As shown below, unscrew the 1 M38 screw. Remove the clip on the chamber and
disconnect the tubes from the filter. Remove the isolation chamber filter.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections are correct;
3. Verify normal operations by starting the analyzer.
10-53
Mechanical System
5. As shown below, unscrew the 2 M38 combination screws on both sides and remove the
cover plate.
6. As shown below, unscrew the 4 M38 combination screws on both sides and remove the
power board PCBA.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;
10-54
Mechanical System
10-55
11 Troubleshooting
Repair Guide
Error Trigger Related Potential
Error Related
Code Mechanism Factors Failure Troubleshooting Procedure
Screen
Control 0x0100 AD is not Control board / Voltage 1. Check if the parameters are
board 0002 within Analog drive & within normal ranges in the
failure [2.44-2.55] board Current Voltage & Current Status screen
Status 2. If so, replace the control
Power board
screen board
and connecting
wires 3. Otherwise, check if the power
board and connecting wires and
the connection between the
analog drive board J2 and
control board J86 are normal
11-1
Troubleshooting
System 0x0100 Check the Button battery / / 1. Check if the button battery is
clock 0004 system clock installed on the digital board
failure is earlier than 2. If the battery is installed,
January 1, replace the battery and reset date
2000 Control board /
and time in the Setup screen.
Save and exit and reset the
analyzer
Diluent 0x0100 The diluent Reagent / Reagent 1. Verify there is no alarm in the
is used 0104 level is lower information Setup Reagent Setup screen
up than 4% setup screen 2. Verify there is sufficient
reagent in the diluent tank
LH lyse 0x0100 The LH lyse Reagent 1. Metering Reagent 1. Verify there is no alarm in the
is used 0105 level is lower information pump failure Setup Reagent Setup screen
up than 10% setup 2. Leaking or screen 2. Verify there is enough reagent
folding lyse in the reagent tank
related tubes
DIFF 0x0100 The DIFF
and valves
lyse is 0106 lyse level is
used up lower than
3%
Waste 0x0100 Float status Waste sensor Unable to Sensor 1. Check if the indicated
level is 0110 assembly change the Status information is as expected from
status
11-2
Troubleshooting
Power 0x0100 56V power is Power board Damaged Voltage 1. Check if the Voltage &
failure 0201 not within power board & Current Current Status screen shows
[47, 60] Status expected information
screen 2. Check if the voltage at the
0x0100 12V power is Power cable Unreliable
test port of the related power
0202 not within power cable
board is as expected
[11.5, 12.5]
3. Check if the connection
0x0100 -12V power Analog drive Damaged
between the power board and
0203 is not within board board
the analog drive board is normal
[-12.5, -11.5]
4. Check if there is no short
0x0100 P12V power Peripherals and Short circuit circuit in analog peripherals,
0204 is not within connections connection power drive
[11.5, 12.5] peripherals and their respective
connections
0x0100 P24V power / /
0205 is not within 5. Replace the analog drive
preheat 0x0100 The preheat preheat bath Damaged Temp.& 1. Verify the corresponding
bath 0401 bath temperature Pressure temperature value from the
tempera temperature sensor Status Status screen
ture reading at the screen 2. First, check if the ambient
Temperature Loose wire
sensor analog drive temperature is overheating
sensor
failure board is 0 or
connection 3. If the temperature reads 0,
70
11-3
Troubleshooting
Optical 0x0100 The optical Optical system Damaged the temperature sensor is
preheat 0x0100 After startup, preheat Damaged Status 1. Enter the Status screen. If the
assembl 0404 the preheat assembly heater screen preheat bath temperature is less
y failure bath than 35 or greater than 55,
Damaged
temperature then there is an assembly failure
temperature
rises less than 2. If the problem persists after
sensor
1 degree per reconnecting the wires, replace
minute for Damaged the preheat assembly
consecutive 3 temperature
minutes switch
Laser 0x0100 Laser current Optical system Damaged Voltage 1. First, check for an abnormal
failure 0809 is not within laser & Current laser current from the Status
[20, 70] Status screen
Laser drive Damaged
screen 2. Open the optical system
board board
shield box and turn the box
Optical system Damaged switch on and off manually. If
shield box
11-4
Troubleshooting
Drive Movement
mechanism limited by
mechanical
interference or
other causes
11-5
Troubleshooting
HGB 0x0100 HGB voltage WBC reaction There is Voltage 1. Check there is no foreign
blank in not within foreign matter & Current
11-6
Troubleshooting
voltage 0801 [3.2, 4.9] bath in the bath Status matter in the WBC bath
abnorm screen 2. Check the bath is correctly
The bath is
al filled with reagent
not filled with
diluent while 3. Verify the HGB blank voltage
measuring from the Voltage & Current
Status screen
Analyzer setup HGB gain
4. Verify the HGB gain settings
setup incorrect
5. Check the HGB assembly
HGB assembly Damaged
HGB
assembly
Fluid 0x0100 MCU reports Hydraulic Related tubes Status 1. Check if the fluid pressure is
pressure 0803 absolute sensor related folded screen close to current local
overloa pressure tubes Valve atmospheric pressure from the
d above 320 blockage or Status screen
kPa failure 2. Check there is no folding
tube, blocked or broken valve in
Optical system Severely
analyzer tubes
blocked
3. Check if there is obstruction
Hydraulic Loose in the sample probe or optical
sensor and connection or system
connection damaged
4. Check if there is loose
sensor
connection in hydraulic sensor
and its connecting wire
Abnor 0x0100 Vacuum is Waste pump Dirt blockage Status 1. Enter the Status screen.
mal 0804 not within the or failure screen Check if the pressure building
vacuum range process is normal (e.g. if the
specified by Related Dirt blockage waste pump is working
the sequence solenoids or failure properly)
11-7
Troubleshooting
Abnor 0x0100 preheat bath Temperature Loose wire or Status 1. Check if the preheat bath
mal 0805 temperature sensor damaged screen temperature and the diluent
tempera out of range connection temperature temperature is as expected from
ture of [expected sensor the Status screen. Determine if
preheat temperature the abnormal status is caused by
bath -1.5, expected too great temperature difference
temperature during diluent replacement
+3] (recoverable, not a problem)
High 0x0100 Optical Fan assembly Damaged fan Status 1. Check if the optical system
analyze 0806 temperature is or loose wire screen temperature is as expected from
r above 40 the Status screen
Analyzer air Air
tempera 2. Check if the fan assembly is
inlet/outlet inlet/outlet
ture working correctly (check for fan
blockage
assembly damage or analog
Optical system Loose wire or drive board damage), and if the
damaged air inlet/outlet is blocked
temperature
3. Solve the problem of the
sensor
11-8
Troubleshooting
Diluent 0x0100 Diluent Fan assembly Damaged fan Status 1. Check if the diluent
tempera 080C temperature or loose wire screen temperature is as expected from
ture above 40 the Status screen
Ambient Room
above 2. Check if the fan assembly is
Temperature temperature
upper working correctly, and if the air
too high
limit inlet/outlet is blocked
Temperature Loose wire or
3. Solve the problem of the
sensor damaged
sensor according to Temperature
connection temperature
Sensor Failure
sensor
4. Check if the ambient
temperature is within the
specified operation temperature
range of the product
11-9
Troubleshooting
bath
Apertur 0x0100 Aperture Aperture Wrong System 1. Run aperture voltage test
e 0902 voltage below aperture Self-test from the System Self-test screen
voltage 17V specification to verify low aperture voltage
is too (temperature 2. If the aperture has been
Diluent Loose wire or
low corrected) replaced, check if there is wrong
temperature damaged
aperture specification,
sensor temperature
installation problem or cracked
assembly sensor
aperture
Abnor 0x0100 Abnormal Flow cell Dirt blockage / 1. Problem 1: Clean the flow
mal 0904 sample cell
optical stream 2. Problem 2: Replace the
system optical system
signal
11-10
12 Debug
12.1 Mechanical Position Adjustment
Figure 1.1 Tooling Materials
Fixture Consumables
Operation Quality Control
Operation Content
Procedure QC Point QC Standard
Click on Service Sample Probe Debug. Enter the
1.1
Debug screen as shown in Figure 1.1.
First, click on the Initial position so that the sample Adjust the
1.2 probe is above the RBC bath, as shown in Figure 1.1. sample probe
position
Click on the WBC bath(or RBC bath) button from Fine tuning of The sample
the screen shown in Figure 1.1. Click the Middle the relative probe is
position button so that the probe wipe is shown. position basically in
Figure 1.2 Figure 1.3 Check if the sample probe is basically in the center of between the the center of
1.3 the bath. If there is any deviation, loosen the four sample probe the bath
screws shown in Figure 1.3 and slightly adjust the and the reaction
bath in left/right and front/back directions so that the bath
sample probe is in the center of the bath. Then
tighten one screw in each direction.
Adjustment screw for front and
back position
Adjust Note:
ment 1. In Figure 1.1, before clicking on Initialization, all the buttons except the Initialization button will be
screw
for left grey. Only after clicking on Initialization, the three buttons in the Sample probe horizontal position
and
right
column will be illuminated and available for use. The Middle position and Down position buttons will
positio not be available until clicking on the WBC bath or RBC bath button.
n
12-1
Debug
12-2
Debug
12-3
Debug
Click ServiceSelf-test from the main Aperture Aperture voltage is normal and
menu bar to enter the Self-test screen, voltage no alarm is triggered.
as shown in Figure 3.1. Click on the
3.4
Aperture voltage button and record the
date generated in the Status Screen in the
record sheet
Installed the shield cover with M38 Tighten the
stainless steel panhead combination screws
3.5
screws and tighten the screws with a
crosshead screwdriver.
Run a count again and observe the sample The relative The relative position between
probe assembly during the whole process. position the sample probe and the
The sample probe shall not interfere with between reaction bath shield box
the shield box while the reaction bath is the sample
3.6
mixing. probe and
the reaction
bath shield
box
Note: The above-mentioned gas bubble refers to big bubble which separates the fluid in the tube.
12-4
13 Commissioning and Verification
After Servicing
Component Name Material ID Commissioning Items
Requirements After
Replacement
Pinaster board(5 051-001159-00 1. Perform data Version
ALL_AM1808) backup according to information is
the prompts correct
2. Check the
corresponding version
information from the
Version Info. screen
Analog Drive Board 115-018411-00 1. Perform HGB gain HGB blank
calibration voltage is 4.5V
2. Recalibrate the
MCV gain
Syringe transfer assembly 115-020624-00 Recalibration Verify the
10ml syringe 115-011902-00 reproducibility
Optical Sensor block of 3101-20-68304
injector
special bolt 3 041-005167-00
10ml syringe FRU 115-015338-00
250ul syringe(with 115-012708-00
nozzle)
Stepping motor 024-000366-00
Sampling Assembly 115-015676-00 1. Move the Verify the
Elevator 801-1805-00013- sampling assembly reproducibility
Motor(2S42Q-05640A) 00 horizontally and
Rotation Motor Position 801-3003-00015- vertically to ensure
Sensor Assembly 00 that the sample line is
sensor package 801-3001-00055- unobstructed with no
00 folding and
13-1
Commissioning and Verification After Servicing
00 pre-amplifier, fluidics
13-2
Commissioning and Verification After Servicing
calibration
4. Recalibration
HGB cound pool module 115-015987-00 1. Mechanical 1. Verify the
commissioning of the reproducibility
relative position 2. HGB blank
between the sample voltage is 4.5V
probe and the bath
2. The waste line for
the reaction bath needs
to be wrapped in the
vertical direction to a
height above the liquid
level with 3mL of
liquid in the bath
3. Perform HGB gain
calibration
Front bath washer 801-3005-00057- 1. Install the aperture 1. Verify the
(molding WH02-205) 00 so that the surface with reproducibility
Microsensor (D50m) 045-000809-00 the tapered bore faces 2. Verify the
the primary bath aperture voltage
2. Recalibration
RBC shielding bottom 042-007064-00 Mechanical position Verify that the
plate welding piece adjustment of RBC sampling assembly
bath will not contact
with the RBC bath
when moving
vertically in the
RBC bath position
HGB FRU 115-018415-00 Perform HGB gain HGB blank
calibration voltage is 4.5V
Vacuum unit 115-015673-00 / Verify that the
Waste pump assembly 115-018416-00 reaction baths can
(FRU) be correctly
drained and
vacuum can be
13-3
Commissioning and Verification After Servicing
correctly built
2-way Valve (Mindray) 801-3201-00002- / 1. Verify the
00 orientation of the
2-way Valve (Mindray) 801-3201-00004- inlet/outlet is
00 correct;
2. The hose shall
be fully inserted;
3. Thick 50 tubes
can not be used
again after
disconnected from
valve ports or
connectors
3-way Valve (Mindray) 801-3201-00003- / 1. Verify the NO,
00 NC and Common
terminals are
connected
correctly;
2. The hose shall
be fully inserted;
3. Thick 50 tubes
can not be used
again after
disconnected from
valve ports or
connectors
LVM valve assembly 115-015675-00 Recalibration 1. Verify the NO,
NC and Common
terminals are
connected
correctly;
2. The hose shall
be fully inserted;
3. Verify the
reproducibility
13-4
Commissioning and Verification After Servicing
13-5
Commissioning and Verification After Servicing
13-6
14 Service BOM
043-000711-00 filter
14-2
Service BOM
110-002557-00 Installer CD
14-3
15 Appendices
A. Fluidic diagram
A-1
B. Connection and Tube
Position
Name in
Material in the
ID the Part NO. Name
type Fluidic
diagram
diagram
1 syringe SR 115-015652-00 syringe assembly D2
2 WBC WBC 115-015987-00 GHB cound pool module B3
3 RBC RBC 115-015986-00 RBC cound pool module B4
4 Vacuum VC 115-015673-00 Vacuum/Pressure Chamber D5
5 Pump LP 115-015674-00 waste water pump subassembly D4
6 probe SPB 3101-20-68488 sampling probe D2
7 probe wipe SPB 3102-20-69178 probe wipe D2
Isolation
chamber1,I
chamber 115-002439-00 Isolation chamber filter C3,C4
solation
8 chamber2
9 Valve SV1 115-010088-00 3-way Valve (Mindray) A4
10 Valve SV2 115-010088-00 3-way Valve (Mindray) A4
mirco solenoid valve of two-way(higher
Valve SV3 115-007667-00 C2
11 operating pressure)
12 Valve SV4 115-010089-00 2-way Valve (Mindray) B2
13 Valve SV5 115-010089-00 2-way Valve (Mindray) C2
14 Valve SV6 115-010089-00 2-way Valve (Mindray) A2
15 Valve SV7 115-010089-00 2-way Valve (Mindray) B5
16 Valve SV8 115-010088-00 3-way Valve (Mindray) A4
17 Valve SV9 115-010089-00 2-way Valve (Mindray) D3
18 Valve SV10 115-010089-00 2-way Valve (Mindray) C3
19 Valve SV11 082-000446-00 Valve.3-way -75~250kPa DC12V C2
20 Valve SV12 115-010089-00 2-way Valve (Mindray) B5
21 Valve SV13 115-010089-00 2-way Valve (Mindray) C5
22 Valve SV14 115-010089-00 2-way Valve (Mindray) C3
23 Valve SV15 115-010089-00 2-way Valve (Mindray) C3
24 Valve SV16 115-010089-00 2-way Valve (Mindray) C2
25 valVe PV18 115-014726-00 TAKASAGO value assem B3
Hydraulic
Sensor 115-017730-00 liquid detect assembly
26 Sensor C2
Preheat
Preheat Bath 115-015672-00 Preheat Bath A3
27 Bath
28 Pump DP1 115-014601-00 1ml Pump(eject) A4
29 Pump DP2 115-014598-00 200ul pump B4
30 Valve CV1 BA40-10-62038 Check Valve A of WU A3
Temperatur
31 Sensor e Sensor 115-015677-00 Diluent temperature testing unit B1
32 Filter LF 0010-10-12408 Inline Filter 43um 1/8 I.D. Tubing A5
33 / / / / /
34 / / / / /
35 Tube T1 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 C1
36 Tube T2 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 C1
37 Tube T3 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 B1
38 Tube T4 0040-10-32301 Tubing. FEP,0.040"X0.066"1feet D2
39 Tube T5 M90-100031--- tube.PTFE,1.7mmIDX2.55mmOD C2
40 Tube T6 M90-100031--- tube.PTFE,1.7mmIDX2.55mmOD C2
41 Tube T7 M90-100031--- tube.PTFE,1.7mmIDX2.55mmOD C2
42 Tube T8 M6G-020007--- Tube OD3mm ID1mm EVA C2
43 Tube T9 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 C1
44 Tube T10 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 C1
45 Tube T11 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 C1
46 Tube T12 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 C2
47 Tube T13 M90-100071--- Tubing.3/32"X5/32",S-50-HL AAX02004,Tygon(20feet)
C2
B-1
Appendices
Position
Name in
Material in the
ID the Part NO. Material description
type Fluidic
diagram
diagram
48 Tube T14 M90-100071--- Tubing.3/32"X5/32",S-50-HL AAX02004,Tygon(20feet)
C3
49 Tube T15 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C3
50 Tube T16 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C4
51 Tube T17 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 A1
52 Tube T18 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 B1
53 Tube T19 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 A4
54 Tube T20 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon A5
55 Tube T21 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 A2
56 Tube T22 M90-100031--- Tube.PTFE,0.066"IDX0.098"OD C2
57 Tube T23 M90-100031--- Tube.PTFE,0.066"IDX0.098"OD B2
58 Tube T24 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 B5
59 Tube T25 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A4
60 Tube T26 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A4
61 Tube T27 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 B3
62 Tube T28 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 B3
63 Tube T29 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 B4
64 Tube T30 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A4
65 Tube T31 M90-100031--- Tube.PTFE,0.066"IDX0.098"OD A3
66 Tube T32 M90-100031--- Tube.PTFE,0.066"IDX0.098"OD A3
67 Tube T33 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD B3
68 Tube T34 M6G-020034--- 3-way valve tubing(1.6*3.2 B3
69 Tube T35 0040-10-32301 Tube.PTFE,0.040"IDX0.066"OD B3
70 Tube T36 3001-10-07069 Tube.1/16"X1/8",S-50-HLAAX02002,Tygon C3
B-2
Appendices
Name in Position
Material
ID the Part NO. Material description in the
type
diagram Fluidic
97 Tube T63 0040-10-32301 Tube.PTFE,0.040"IDX0.066"OD B3
98 Tube T64 M90-100031--- Tube.PTFE,0.066"IDX0.098"OD B2
99 Tube T65 0040-10-32301 Tube.PTFE,0.040"IDX0.066"OD B2
100 Tube T66 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon C5
101 Tube T67 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C5
102 Tube T68 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon D5
103 Tube T69 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon D4
104 Tube T70 M6G-020034--- 3-way valve tubing(1.6*3.2) C5
105 Tube T71 M6G-020034--- 3-way valve tubing(1.6*3.2) B5
106 Tube T72 M6G-020055--- Tube.TPU,ID1/8,OD1/4,clear B5
107 Tube T73 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon D5
108 Tube T74 M6G-020055--- Tube.TPU,ID1/8,OD1/4,clear D4
109 Tube T75 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon B3
110 Tube T76 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon A4
111 Tube T77 M6G-020006--- Tube.Silicone,1/16"X3/16",TYGON 3350 B2
112 Tube T78 0040-10-32301 Tube.PTFE,0.040"IDX0.066"OD B2
113 Tube T79 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon B2
114 Transit tube J1 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet D2
115 Transit tube J2 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet C2
116 Transit tube J3 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran C2
117 Transit tube J4 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran C2
118 Transit tube J5 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran C2
119 Transit tube J6 M6G-020006--- Tube.Silicone,1/16"X3/16",TYGON 3350 C2
120 Transit tube J7 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A3
121 Transit tube J8 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A3
122 Transit tube J9 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran B3
123 Transit tube J10 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet B3
124 Transit tube J11 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet B3
125 Transit tube J12 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet B2
126 Transit tube J13 0030-20-13339 Transit tube(1.5mm-3mm)(Mould MR13339) B2
127 Transit tube J14 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A4
128 Transit tube J15 0030-20-13339 Transit tube(1.5mm-3mm)(Mould MR13339) A4
129 Transit tube J16 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon B3
130 Transit tube J17 M6G-020006--- Tube.Silicone,1/16"X3/16",TYGON 3350 B3
131 Transit tube J18 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon B4
132 Transit tube J19 M6G-020006--- Tube.Silicone,1/16"X3/16",TYGON 3350 B4
133 Transit tube J20 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet B2
134 Transit tube J21 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet B3
135 Transit tube J22 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran B2
136 Transit tube J23 0030-20-13339 Transit tube(1.5mm-3mm)(MouldMR13339) B2
137 Transit tube J24 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet B2
138 Transit tube J25 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran B2
139 Transit tube J26 0030-20-13339 Transit tube(1.5mm-3mm)(MouldMR13339) B2
140 Transit tube J27 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran C2
141 Transit tube J28 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran C2
142 Transit tube J29 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A3
143 Transit tube J30 0030-20-13339 Transit tube(1.5mm-3mm)(MouldMR13339) B2
144 Transit tube J31 0030-20-13339 Transit tube(1.5mm-3mm)(MouldMR13339) B2
B-3
Appendices
Name
Position
in
Material in the
ID the Part NO. Material description
type fluidic
diagr
diagram
am
145 Connection C1 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C1
146 Connection C2 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C1
147 Connection C3 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C1
148 Connection C4 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C1
149 Connection C5 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C3
150 Connection C6 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 B5
151 Connection C7 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C5
152 Connection C8 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C5
153 Connection C9 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C4
154 Connection C10 043-000880-00 8.5mm three way connector B3
155 Connection C11 M90-100028-03 Connection.Y,400Barb,3/32"ID,White Nylon B3
156 Connection C12 M90-100026--- Nylon plug (located at the reagent inlet) B3
157 Connection C13 M90-100026--- Nylon plug (located at the reagent inlet) B4
158 Connection C14 043-000892-00 straight tube tie-in A4
159 Connection C15 043-000892-00 straight tube tie-in A4
160 Connection C16 M90-100027--- Connection.Straight Through Reduction,1/8"&3/32"ID C5
161 Connection C17 M90-100027--- Connection.Straight Through Reduction,1/8"&3/32"ID A5
162 Connection C18 082-001140-00 Connection.Male Luer 2mm"ID natural PVDF B5
163 Connection C19 082-001141-00 Connection.FemaleLuer,1/4-28UNF,1/8"ID B5
164 Connection C20 082-001140-00 Connection.Male Luer 2mm"ID natural PVDF B5
165 Connection C21 082-001141-00 Connection.FemaleLuer,1/4-28UNF,1/8"ID B5
166 Connection C22 M90-100009--- Connection.FemaleLuer,1/4-28UNF,1/8"ID A5
167 Connection C23 M90-100025--- Connection.Male Luer,1/8"Barb,White Nylon A5
168 Connection C24 M90-100009--- Connection.FemaleLuer,1/4-28UNF,1/8"ID B4
169 Connection C25 M90-100025--- Connection.Male Luer,1/8"Barb,White Nylon B4
170 Connection C26 M90-100100--- Connection.Elbow,400Barb,3/32"ID,White,L420-1 C2
171 Connection C27 M90-100100--- Connection.Elbow,400Barb,3/32"ID,White,L420-1 A2
172 Tube T80 0040-10-32301 Tube.PTFE,0.040"IDX0.066"OD A4
173 Transit tube J32 0030-20-13339 Transit tube(1.5mm-3mm)(Mould MR13339) A4
174 Transit tube J33 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran C2
175 Transit tube J34 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran C2
176 Tube T81 M6G-020056--- Tube.EVA,ID:1/16,OD1/8clear B5
177 Tube T82 M6G-020056--- Tube.EVA,ID:1/16,OD1/8clear A5
B-4
C. Hardware block diagram
C-1
D. Cables and Wires
Material ID Name Connection
009-002857-00 Heater connecting wire Connects A-J21 with heater and
temperature switch
009-002858-00 Temperature sensor Connects A-J10 with temperature
connecting wire sensor
009-002863-00 Indicator board connecting Connects B-J78 with indicator board
wire
009-002865-00 Analog board supervisory Connects A-J2 and B-J86
signal wire
009-002866-00 Optical signal output wire Connects A-J4 and B-J85
009-002867-00 Analog main control board Connects A-J31 and B-J81
SPI signal wire
009-002869-00 Optical control signal wire Connects A-J3 and B-J77
009-002870-00 Syringe motor connecting Connects A-J32 and syringe motor
wire
009-002871-00 Digital power connecting Connects C-J1/C-J2 and A-J22/B-J68
wire
009-002872-00 Analog power connecting Connects A-J8 and C-J3 C-J4
wire
009-002875-00 Sampling motor connecting Connects A-J24 and sampling motor
wire
009-002877-00 Electromagnetic metering Connects A-J16 and electromagnetic
pump connecting wire metering pump
009-002878-00 Valve connecting wire Connects A-J14 A-J15 and valves
009-002880-00 Waste pump connecting Connects A-J20 and waste pump
wire
009-002881-00 Float switch connecting Connects A-J1 and float switch
wire
009-002882-00 Sampling photocoupler Connects A-J12 and sampling
connecting wire photocoupler
009-002911-00 Touchscreen control cable Connects B-J67 and touchscreen drive
board
009-002913-00 LCD signal wire Connects B-J4/B-J16 and LCD/backlight
009-002967-00 RBC connecting wire Connects A-J6 and RBC bath
009-002988-00 Safety ground /
009-002989-00 Component ground /
009-003176-00 Syringe assembly Connects A-J13 and syringe assembly
photocoupler connecting photocoupler
wire
009-003322-00 Start key connecting wire Connects B-J99 and start key
009-003381-00 Fan adapter cable Connects A-J17 A-J18 and fan
009-003642-00 Hydraulic sensor adapter Connects A-J11 and hydraulic sensor
cable
2800-20-28762 HGB connecting wire Connects A-J5 and HGB bath
009-004057-00 LH&DIFF detect board wire Connects A-J26 and Liquid detect
board PCBA
Note: A refers to the analog drive board, B refers to the main control board, C refers to the power
board
E. Appendix Table
Product Model SERIAL NUMBER
No. Inspection Item Description Requirement Result Conclusion
Refer to related
Power cable
1 Electrical connection graphic files in OK NG PASS FAIL
connection
the appendix
Red indicates
2 Indicator color Color problems, green OK NG PASS FAIL
indicates OK
Alarms when
3 Indicator sound Sound OK NG PASS FAIL
problems exist
Touchscreen
4 Touchscreen Touchscreen calibration OK NG PASS FAIL
completed
Correct time and
5 Time and date Enter time and date OK NG PASS FAIL
date entry
Version and
6 Version configuration In latest versions OK NG PASS FAIL
information
Syringe and sampling Syringe and
7 mechanism self-test sampling Normal operation OK NG PASS FAIL
mechanism self-test
E-1
Connection and Tube
Counting channel
22 Aperture voltage [16, 21]V PASS FAIL
measurement
Counting channel splash when WBC
measurement bubbling or not, No splash and no
23 OK NG PASS FAIL
bubbles on filling contact
tube or not
Counting channel splash when RBC
measurement bubbling or not, No splash and no
24 OK NG PASS FAIL
bubbles on filling contact
tube or not
Counting channel WBC bath can be
25 measurement drained correctly or Drained correctly OK NG PASS FAIL
not
Counting channel RBC bath can be
26 measurement drained correctly or Drained correctly OK NG PASS FAIL
not
Counting channel
27 WBC bath wall No residue OK NG PASS FAIL
measurement
Counting channel
28 RBC bath wall No residue OK NG PASS FAIL
measurement
Counting channel RBC bath shield box
29 Screws tightened OK NG PASS FAIL
measurement installation
Sample probe
Counting channel Sample probe
30 moves with no OK NG PASS FAIL
measurement movement
interference
Sample probe
Counting channel tube does not
31 Sample probe tube OK NG PASS FAIL
measurement interfere with
other structures
Perform
Refer to the user's
32 Maintenance maintenance and OK NG PASS FAIL
manual
cleaning
Reaction bath voltage
RBC aperture
33 measurement and [16, 21]V PASS FAIL
voltage
setup
Analyzer status
34 RBC 0.021012/L PASS FAIL
verification - count
Analyzer status
35 HGB 1g/L PASS FAIL
verification - count
Analyzer status
36 HCT 0.5% PASS FAIL
verification - count
Analyzer status
37 PLT 10109/L PASS FAIL
verification - count
Connect the optical Refer to the
Optical system tube
38 tubes and fill with service OK NG PASS FAIL
connection
fluids instructions
E-2
Connection and Tube
E-3
P/N: 046-008515-00(3.0)