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Chapter I
Introduction
Thermo Orion provides pH and ISE meters for every application from pH
measurements to advanced Ion Selective Electrode work. This manual
describes the operation and use of the Thermo Orion Model 410Aplus
pH/Temperature Meter, Model 420Aplus pH/mV/Temperature Meter, the
Model 520Aplus Advanced pH/mV/Temperature Meter, the Model
525Aplus Dual Channel pH/mV/Temperature Meter, the Model 710Aplus
pH/ISE Meter, the Model 720Aplus Dual Channel pH/ISE Meter, and the
Model 920Aplus Advanced pH/ISE Meter.
All meters are microprocessor-controlled and feature pH autocalibration,
sealed keypads, simultaneous temperature display and many other features.
The Models 410Aplus, 420Aplus and 710Aplus feature a custom LCD
display. The Models 520Aplus, 525Aplus, 720Aplus, and 920Aplus feature
a two color vacuum fluorescent display with a separate prompt line that
explains each step during calibration and measurement.
Introduction 1
Benchtop pH/ISE Meter Instruction Manual
2
Benchtop pH/ISE Meter Instruction Manual
Chapter II
General Information
Models 410Aplus, 420Aplus, 710Aplus
General Information
The Model 410Aplus is a pH/Temperature Meter, the Model 420Aplus is a
pH/mV/Temperature Meter and the Model 710Aplus is a pH/ISE
mV/Temperature Meter for general laboratory use. These meters feature:
(1) a custom LCD display, which simultaneously displays mode, results
and temperature; (2) a sealed keypad with tactile and audible feedback;
and (3) an RS232 port for use with the Thermo Orion 900A printer or other
serial peripheral devices.
Models 520Aplus, 525Aplus, 720Aplus, 920Aplus
General Information
The Model 520Aplus is a pH/mV/Temperature Meter, Model 525Aplus
is an Advanced pH/mV/Temperature Meter, 720Aplus is a
pH/ISE/mV/Temperature Meter and 920Aplus is an Advanced
pH/ISE/mV/Temperature meter for general or advanced laboratory use.
These meters feature: (1) a two color vacuum fluorescent display; (2) a
sealed keypad with tactile and audible feedback; and (3) an RS232 port for
use with Thermo Orion 900A printer or other serial peripheral devices. The
Models 525Aplus, 720Aplus and 920Aplus feature dual electrode inputs.
General Information 3
Benchtop pH/ISE Meter Instruction Manual
Chapter III
Set Up, Self-Test and Check-Out
Procedures
A. Models 410Aplus Set Up and Self-Test
NOTE: Use this procedure when the instrument is
first received and whenever troubleshooting
becomes necessary.
1. Connect BNC shorting cap (Thermo Orion Catalog No. 090045)
supplied with meter to sensing electrode input.
2. Disconnect the ATC probe.
3. Plug line (Thermo Orion Catalog No. 020125 for 110V, Thermo Orion
Catalog No. 020130 for 220V) into an appropriate wall outlet then
securely into meter power receptacle.
NOTE: Firmly push the power adapter into the jack
on the back of the meter.
4. Press and hold yes while pressing power. The instrument
automatically performs electronic and hardware diagnostic tests. See
the explanation in the Self-Test Section of the troubleshooting guide
for a more detailed explanation.
a. When O appears in the lower field, press each key
including power. A numeric digit will be displayed for
each keypress.
b. During test 8, the meter will turn off. To restart meter press power.
ready
ON C timer
OFF ATC
4 7 10 Man
SETUP:
S-1 RESOLUTION mode
S-2 SLOPE
S-3 PRINT OPTIONS
S-4 CAL BUFFER OPTION
yes
ERROR CODES:
E-20 OUT OF RANGE
E-21 AUTO BUFFER ERROR
E-22 CAL STANDARD ERROR no
print
E-23 BAD SLOPE
E-29 PRINTER ERROR
E-31 BAD VALUE
power
See Manual for other Error Codes.
pH mV Rel mV Conc
SETUP:
yes no
1-1 READY
1-2 HOLD
1-3 BEEP
1-4 AUTOSHUTOFF
2-1 SLOPE
2-2 RESOLUTION measure
2-3 ISOPOINT timer print
2-4 RESET
3-1 TIMER INTERVAL
3-2 REMAINING TIME
3-3 SET REAL TIME
3-4 SET DATE
4-H HIGH ALARM LIMIT
mode
4-L LOW ALARM LIMIT setup cal
5-1 PRINT MODE: 1 = MAN, 2 = READY, 3 = TIMED
5-2 PRINT INTERVAL
6-1 DATA LOG MODE: 1 = MAN, 2 = READY, 3 = TIMED, 0 = OFF
6-2 DATA LOG INTERVAL
6-3 DOWNLOAD DATA
6-4 DATA CLEAR power 2nd
See Manual for Error Codes.
For Model 420Aplus, the meter is now ready for use with electrodes.
Remove the shorting cap.
5. For Model 710Aplus only
With the shorting cap still connected, press mode until the
concentration mode indicator is displayed. The display should
read 1.00.
NOTE: If this is the first time this procedure has been
performed the reading should be 1.00.
a. If not, press 2nd cal. At the P1 prompt, scroll until the display
reads 1.00. Press yes.
b. Press measure. SLP and 59.2 should be displayed. If so,
press yes.
c. If not, scroll until the display reads 59.2, then press yes.
1 2 3
yes mode calibrate measure
4 5 6
no hold resolution
channel
slope
7 8 9
1st print
data log
activate
set timer
set temp
ready
mV
2nd
+-
date/time
0
standby
.
data/print
beep options modes
alarm
model 525
1 2 3
yes mode calibrate
cal edit
measure
stability
4 5 6
no hold
incr tech
resolution
channel
slope
electrode
ID
7 8 9
1st print
data log
activate
set timer
set temp
ready
mV
2nd
+-
date/time
0
standby
.
data/print
beep options modes
alarm
model 920
Instrument Set Up
The meter can be adjusted to provide you an optimal viewing angle via the
flip stand. To adjust the viewing angle, turn the instrument over and place
your thumbs on the inside of the stand sides. As you lift the stand, gently
push the sides of the stand out. This will lock the stand at a 90 angle,
providing easy viewing from across the lab. To adjust to the last viewing
angle, continue to gently push the sides of the stand out as you lift the stand
to its furthest point.
Chapter IV
Rear Panel and Electrode Connections
A. Rear Panel
Inputs Use
(1.) Input Sensing electrode jack. Inputs accept pH,
1 and 2 ion selective, and redox electrodes with
BNC connectors. (Input 1 illustrated with
shorting cap connected, input 2 illustrated
with shorting cap disconnected).
(2.) Ref 1 Reference electrode jacks. Inputs accept
Ref 2 standard pin-tip connectors.
(3.) Gnd. Earth ground jack, accepts standard
pin-tip connectors.
(4.) ATC Automatic Temperature Compensator jack,
accepts thermistor-type ATC probe with
DIN connector.
(5.) KF Polarizing current source for Karl Fischer
titrations. Jack accepts standard pin-
tip connectors.
(6.) Rec Recorder jack. Accepts 2.5 mm audio jack
for strip chart recorder connection. Tip is
output, ring is ground.
(7.) Power Power receptacle. Accepts input connector
from Thermo Orion supplied line converter.
(8.) BNC BNC Shorting Cap.
Power
9V DC ATC/DIN Ref. Input Rec. Gnd. ATC/DIN KF Input 1 Ref.1 Input 2 Ref. 2
2
7 1
6
Figure #5 Rear Panel
3 5
4 8
NOTE: The Models 410Aplus, 420Aplus, 520Aplus and
710Aplus contain only Sensing Electrode Input 1 and
Reference Electrode Input 1. On the Models 410Aplus,
420Aplus, and 710Aplus the inputs are found on the left
side of the rear panel.
B. Electrode Connections
1. Attach electrodes with BNC connectors to sensor input by sliding
connector onto input, pushing down and turning clockwise to lock into
position. Connect reference electrodes with pin tip connectors by
pushing connector straight into reference input.
NOTE: If using a combination electrode with a BNC
connector, a reference electrode is not used.
2. Attach the ATC probe to the ATC jack by sliding the connector
straight on until it is firmly in place. The connector has a special
sealing mechanism, which is engaged, when the connector is
properly attached.
Chapter V
Calibration and Measurement of pH
A. General Information on pH Calibration
Temperature Compensation
pH measurements on the Model 410Aplus are made with Automatic
Temperature Compensation. pH measurements on the Models 420Aplus,
520Aplus, 525Aplus, 710Aplus, 720Aplus, and 920Aplus may be made
with either Automatic or Manual Temperature Compensation.
For Automatic Temperature Compensation, an ATC probe must be used.
Plug in the ATC probe and the meter will display temperature corrected pH
results in the main display.
For Manual Temperature Compensation with Models 520Aplus,
525Aplus, 720Aplus, and 920Aplus disconnect the temperature probe.
Temperature values can be entered manually by pressing set temp.
Temperature corrected pH values, based on the manually entered
temperature, will be displayed in the main field.
For Manual Temperature Compensation with Models 420Aplus and
710Aplus disconnect the temperature probe. Temperature values can be
entered manually by pressing or while in measure mode. The value
will be displayed in the lower field. Temperature corrected pH values based
on the manually entered temperature will be displayed in the main field.
pH Calibration Procedure
1. Connect electrode(s) to meter. Choose buffers that will bracket the
expected sample pH.
2. Rinse electrode(s) and place into buffer.
3. Press mode until CALIBRATE is displayed.
4. The last buffer sequence used will be displayed. Press yes to use this
sequence or no to scroll through choices.
5. Buffer indicator along bottom of the display will indicate the buffer
chosen. P1 will be displayed in lower display field and buffer reading
will be displayed in the main field. When READY is displayed,
indicating electrode stability, press yes to accept the displayed value.
If performing a manual calibration, press no to change each digit until
the correct pH value is displayed then press yes to accept.
6. The temperature corrected value for that buffer is automatically
entered into the memory of the meter. P2 will be displayed in the
lower display field indicating the meter is ready for the second buffer.
The buffer indicator along the bottom of the display will indicate the
second buffer of the calibration sequence selected.
7. Repeat steps 2 and 5 for each buffer.
8. After the buffer value for the last buffer has been entered, press
measure. The electrode slope will be displayed. SLP appears in the
lower field while the actual electrode slope, in percent, appears in the
main field. Press yes, the meter will automatically advance to the
measure mode. MEASURE is displayed above the main field.
9. Rinse electrode(s) and place into sample. Record pH directly from the
main meter display when READY is displayed. Temperature is
displayed in the lower field.
Manual Calibration
To calibrate with buffers other than 4.01, 7.00, or 10.01, use the manual
calibration technique. The chosen buffers must be greater than one (1) pH
unit but less than four (4) pH units from the next closest buffer. The
calibration sequence is the same as autocalibration except that the buffer
values are scrolled in and then entered.
NOTE: For manual calibration you must use or .
Even if the value displayed is the correct value for your
buffer, you must press a scroll key to start the editing
process. Press yes to accept each digit. Otherwise
the meter assumes autocalibration is being performed.
pH Calibration Procedure
1. Connect electrode(s) to meter. Choose buffers that will bracket the
expected sample pH.
2. Press mode until the pH mode indicator is displayed.
3. Rinse electrode(s) and place into buffer.
4. Press 2nd then cal to begin calibration. The date and time of the last
calibration will be displayed.
5. When READY is displayed next to the reading, indicating electrode
stability, the reading will flash. Press yes. For manual calibration
use or to select the correct value, then press yes to accept each
digit. After accepting each digit, press yes store buffer value. The
meter display freezes for three seconds. The meter automatically
switches to buffer two, indicated by the P2 on the display.
6. Repeat steps 3 through 5 for each buffer.
7. After entering the final buffer value, press measure. The electrode
slope will be displayed. SLP appears in the lower field while the
actual electrode slope, in percent, appears in the main field. After the
third buffer point, the meter automatically displays the calibration
slope and advances to the measure mode. MEASURE is displayed
above the main field.
8. Rinse electrode(s) and place into sample. Record pH directly from the
main meter display. Temperature is displayed in the lower field
when the READY prompt displays.
pH Calibration Procedure
1. Connect electrode(s) to meter. Choose buffer(s), which is closest to or
will bracket the expected sample pH.
2. Press calibrate. Calibrate is displayed. The time and date of the last
calibration are displayed.
3. After a few seconds ENTER NO. BUFFERS is displayed. Enter the
number of buffers; 1, 2, or 3, and press yes.
4. Rinse electrode(s) and place into first buffer When the electrode signal
is stable, the meter will display the temperature corrected value for
the buffer.
5. When READY CAL AS is displayed, press yes if the value is correct
for your buffer. If performing a manual calibration, use the numeric
keys to change the value, then press yes.
6. If 2 or 3 buffers were selected, repeat steps 4 and 5 for each buffer.
7a. If one buffer was selected, the actual slope value in memory is
displayed at the SLOPE prompt. Press yes if correct, or enter correct
value and then press yes. If actual slope is unknown, enter 100.0 or
perform a two point calibration.
7b. If two or three buffers were selected, the actual electrode slope is
displayed at the SLOPE prompt. If more than two buffers were used,
the slope displayed is an average of the different segments.
8. The meter automatically proceeds to measure mode.
9. Rinse electrode(s) and place into sample. Record pH value from the
main field when READY is indicated or when the electrode signal is
stable. Temperature is displayed on the prompt line.
pH Calibration Procedure
1. Press 2nd then channel until the correct input channel is selected.
2. Press mode until the pH mode indicator is displayed.
3. Press calibrate. CALIBRATE is displayed. The time and date of the
last calibration are displayed.
4. After a few seconds ENTER NO. BUFFERS is displayed. Enter the
number of buffers; 1, 2, or 3, then press yes.
5. When the BUFFER 1 prompt appears, rinse electrode(s) and place
into first buffer. Wait for a stable display.
6. When the electrode signal has stabilized, the READY CAL AS
prompt will appear. Press yes if the value is correct for your buffer. If
performing a manual calibration, use the numeric keys to change the
value, then press yes.
7. If more than one buffer was selected, repeat steps 5 and 6 for
each buffer.
8a. If one buffer was selected, the current electrode slope in memory is
displayed at the SLOPE prompt. Press yes, or use numeric keys to
enter correct value, then press yes. If actual slope is unknown, enter
100.0 or perform a two buffer calibration.
8b. If two or more buffers were selected, the average electrode slope is
displayed at the SLOPE prompt.
9. The meter automatically proceeds to MEASURE mode.
10. Rinse electrode(s) and place into sample. Record the pH value when
READY is indicated or when the electrode signal is stable.
Temperature is displayed in the prompt line.
Manual Calibration
To calibrate with buffers other than 1.68, 4.01, 7.00, 10.01, or 12.46, use
the manual calibration technique. The calibration sequence is the same as
autocalibration except buffer values are scrolled in and then entered.
NOTE: For manual calibration, you must use the scroll
or keys. Even if the value displayed is the correct
value for your buffer you must press a scroll key to
start the editing process. Press yes to accept each
digit. Otherwise, the meter assumes autocalibration is
being performed.
Multipoint pH Calibration
Up to a five (5) point calibration can be performed on the Model 710Aplus
Meter. Both autocalibration and manual calibration may be used within the
same calibration curve. For example, autocalibration may be used with the
1.68, 7.00, and 10.01 buffers while manual calibration may be used with
3.78 and 9.18 buffers.
pH Calibration Procedure
1. Connect electrode(s) to meter. Choose buffers that will bracket the
expected sample pH.
2. Press mode until the pH mode indicator is displayed.
3. Rinse electrode(s) and place into buffer.
4. Press 2nd then cal to begin calibration. CALIBRATE is displayed
above the main field. The date and time of the last calibration will
be displayed.
5. When the electrode is stable, the READY prompt will be displayed
and the temperature corrected value for the buffer is displayed. Press
yes. If performing a manual calibration, press or to change the
value, then press yes. The buffer value is stored and meter display
freezes for three seconds. The meter automatically switches to buffer
two, indicated by the P2 on the display in the lower field.
6. Repeat steps 3 and 5 for each buffer.
7. After the buffer value for the last buffer has been entered, the
electrode slope will be displayed automatically. If doing a manual
calibration or less than a 5 point autocalibration, press measure. SLP
appears in the lower field while the actual electrode slope, in percent,
appears in the main field. The meter automatically advances to the
measure mode. MEASURE is displayed above the main field.
8. Rinse electrode(s) and place into sample. Record pH directly from the
main meter display when the READY prompt displays. Temperature
is displayed in the lower field.
pH Calibration Procedure
1. Press 2nd then channel until the correct input channel is selected.
2. Press mode until the pH mode indicator is displayed.
3. Press calibrate. CALIBRATE is displayed. The time and date of the
last calibration are displayed.
4. After a few seconds ENTER NO. BUFFERS is displayed. Enter the
number of buffers 1, 2, 3, 4 or 5, then press yes.
5. When the BUFFER 1 prompt appears, rinse electrode(s) and place into
first buffer. Wait for a stable display.
6. When the electrode signal has stabilized, the READY CAL AS
prompt will appear. Press yes if the value is correct for your buffer. If
performing a manual calibration, use the numeric keys to change the
value. Press yes.
7. If more than one buffer was selected, repeat steps 5 and 6 for
each buffer.
8a. If one buffer was selected, the current electrode slope in memory is
displayed at the SLOPE prompt. Press yes, or use numeric keys to
enter correct value, then press yes. If actual slope is unknown, enter
100.0 or perform a two buffer calibration.
8b. If two or more buffers were selected, the average electrode slope is
displayed at the SLOPE prompt.
9. The meter automatically proceeds to measure mode.
10. Rinse electrode(s) and place into sample. Record the pH value when
READY is indicated or when the electrode signal is stable.
Temperature is displayed in the prompt line.
Chapter VI
Calibration and Measurement of
Concentration
Models 710Aplus, 720Aplus, 920Aplus
A. General Information
A one, two, or multipoint calibration should be performed before
concentration is measured. It is recommended that a two point standard
calibration be performed at the beginning of each day and every time
electrodes are changed to determine the slope of the electrode. This serves a
dual purpose, determining if the electrode is working properly and storing
the slope value into memory. Perform a calibration with one standard every
two hours to compensate for possible electrode drift. Use a fresh aliquot
from one of the standards used in the initial calibration. During calibration,
always use the most dilute standard first, the meter will automatically
recognize slope direction (i.e. will recognize anion or cation electrodes).
Standards should bracket the sample range and be in the same
concentration units.
Units
Any convenient units of concentration units can be used. For example:
molarity, ppm, %, etc.
Temperature
Allow all samples and standards to reach the same temperature before
measurement, or use temperature compensation if the electrode isopotential
point is known. (Model 920Aplus only)
Slope
Up to a five point calibration may be performed. The instrument uses a
point-to-point calibration scheme. The meter stores, in memory, the
different electrode slopes for each portion of the calibration curve. When
measuring in a particular region of the curve, the electrode slope for that
region is employed in the calculation of sample concentration. The
electrode slope, displayed after calibration, is the average slope for all the
segments of the entire calibration curve. Use of this scheme increases
accuracy in the different regions of the calibration curve. However, the
electrode slope displayed may be lower than normal, especially if standards
close to the electrode limit of detection are used in the calibration.
Autoblank Correction
Blank correction occurs automatically when calibrating with three or more
standards. This feature automatically calculates and corrects for
background levels of the species of interest. The standards used for
calibration do not need to include a blank. This improves the results
because typical blanks contain low levels of the species to be measured,
making them unstable and difficult to measure accurately. To take
advantage of this feature select three or more standards for calibration. If
the profile of the calibration curve does not indicate a background level of
ion concentration, then blank correction will not be invoked and the
standard point-to-point scheme will be used. Refer to Appendix D for more
detailed information.
36 Special Features
Benchtop pH/ISE Meter Instruction Manual
Chapter VII
920Aplus Special Features
A. Calibration Edit
The calibration edit feature allows you to edit a pH or concentration
calibration curve without repeating the entire procedure. This is useful in
multipoint calibrations if an entry mistake was made on one of the points.
The meter will automatically recalculate all calibration values. There are
two options in the cal edit function: Remeasure or Edit.
Remeasure allows you to remeasure a particular calibration point by
placing electrode(s) in the standard (or buffer), then entering the
correct value.
Edit has two choices: change or delete. Select change if a data entry error
was made, but electrode(s) were in the correct standard at the time of
calibration. Delete removes that point from the calibration curve.
Cal edit presents all calibration points in memory for editing. Respond to
the prompts and edit as needed. Cal edit may be performed for both pH and
ISE calibrations.
If no previous calibration has been performed the meter will prompt, NO
CALIBRATION, then return to MEASURE mode. Otherwise, follow the
prompts to edit the calibration. The example provided here uses a
concentration calibration. A pH calibration would substitute the word
BUFFER for STD.
Special Features 37
Benchtop pH/ISE Meter Instruction Manual
Cal Edit
38 Special Features
Benchtop pH/ISE Meter Instruction Manual
Special Features 39
Benchtop pH/ISE Meter Instruction Manual
40 Special Features
Benchtop pH/ISE Meter Instruction Manual
Set-up
1. Refer to the appropriate electrode instruction manual for preparation
of sensing and reference electrode(s), required solutions (ionic strength
adjusters, standards...), and any special requirements.
2. Securely connect electrode(s) to either input 1 or 2. The number of
the input in use will appear directly before the electrode ID. For
example, 1-Cl -. Select the appropriate input by pressing
2nd then channel.
3. Identify the electrode in use by pressing 2nd then electrode id and set
up other instrument operating parameters.
4. If a single incremental technique is to be performed, determine the
electrode slope from a previous calibration or as directed in the
electrode instruction manual.
Single Incremental Techniques
Single incremental techniques are useful for occasional samples or samples
with complex matrices. Another advantage of the single incremental
techniques is that the electrodes are not moved from solution to solution
during calibration and measurement. This eliminates errors due to solution
carryover and static charge disturbance of the electrode membrane.
It is especially important to be sure that any complexing agent is present in
great excess or not at all. The ratio of free ion to complexed ion must
remain constant over the addition. An indication that a complexing agent is
present, but not in great enough quantity to maintain a constant
free/complexed ion ratio, is an abnormally high or low slope. If this is
a suspected problem, an excess of complexing or decomplexing agent
may be added to the original solution in the beaker prior to the
incremental addition.
Special Features 41
Benchtop pH/ISE Meter Instruction Manual
42 Special Features
Benchtop pH/ISE Meter Instruction Manual
Select 2-SUBTR for the known subtraction procedure then follow the
meter prompts.
Special Features 43
Benchtop pH/ISE Meter Instruction Manual
44 Special Features
Benchtop pH/ISE Meter Instruction Manual
Special Features 45
Benchtop pH/ISE Meter Instruction Manual
46 Special Features
Benchtop pH/ISE Meter Instruction Manual
Chapter VIII
Other Measurement Models
A. Temperature Measurement
The meters display temperature in the range -5.0 to +105C simultaneously
with sample results. Whenever an ATC probe is connected, live temperature
measurements are displayed in the prompt line. For results greater than
100C the tenth digit is deleted. On the Model 920Aplus Meter the
temperature appears after the channel designation and electrode ID.
12. Insert the reservoir (funnel) into a BOD bottle containing enough
water to just cover the bottom. Insert the electrode, making sure
that the electrode tip is not immersed in the water and does not have
water droplets clinging to the outside of the membrane. Let stand
approximately 30 minutes to ensure water saturation of air in the
BOD bottle. This bottle should be used for storage between
measurements.
13. Turn the electrode mode switch to the AIR position. If measurements
are being made at sea level, use the AIR calibration control on the
electrode to set the pH meter reading to the prevailing barometric
pressure in mm Hg (divided by 100). If the barometric pressure is
unknown, the elevation is above sea level or the sample has a salinity
greater than two parts per thousand, consult Table 1 found in the
Model 9708 Instruction Manual to obtain the correct AIR
setting. Turn the electrode mode switch to H2O for sample analysis.
14. Record direct ppm reading from meter.
Chapter IX
Use with Accessories
A. Use with Printers and Computers
The Models 410Aplus and 420Aplus can send information to a printer or
computer using the RS232 port.
Models 520Aplus, 525Aplus, 710Aplus, 720Aplus, and 920Aplus have a
bi-directional interface to be used with printers, computers and other serial
peripheral devices. Thermo Orion provides a cable (Cat. No. 0ACBL0) for
interfacing your meter to an IBM PC or compatible device. For other
computers and communication devices, a suitable cable must be fabricated.
Use the information below to interface your meter and serial peripheral
device.
Command Print
Prints current display.
Ready Print
Prints whenever the READY indicator is displayed.
Timed Print
Prints at selected timed intervals.
The following is an example of a program, written in Basic, which monitors pH data every five
seconds and prints the data if the pH value is less than five.
NOTE: help and rem are not displayed in the help menu.
The following is an example of a program, written in Basic, which monitors pH data every five
seconds and prints the data if the pH value is less than five.
Chapter X
Troubleshooting
A. Self-Test
The self-test is performed by holding yes down while the meter is being
powered on.
NOTE: Before starting the self-test securely attach the
shorting cap to the electrode input(s).
During the self-test the meter will display the number of the test being
performed. If any problems are found, an operator assistance code
corresponding to the test that failed will be displayed. See Table 1 under
operator assistance codes. Should an operator assistance code appear
during self test, press yes to acknowledge and continue the test. Check to
make sure the shorting cap is securely attached then repeat the test. If the
problem persists, contact Thermo Orions Technical Service Department at
800-225-1480.
Models 410Aplus, 420Aplus and 710Aplus: During the self-test the
meter displays the code 7E57 in the main display and the number of the
test being performed in the lower display. Additional meter displays occur
depending on the test being performed.
Models 520Aplus, 525Aplus, 720Aplus, and 920Aplus: During the self
test the meter displays the number of the test in the prompt line. Example:
TEST 1
NOTE: Ensure shorting cap is on the meter prior to self-test.
Test Function/Display Sequence
TEST #1 Segment Display Checks meter display. Ensure all
segments are lit at this time.
TEST #2 RAM Check Verifies the external RAM is
functioning properly.
TEST #3 External Input 1 Checks the electrode input channel
1. The shorting cap must be
connected for this test to pass
properly.
TEST #4 External Input 2 Checks the electrode input channel
2. The shorting cap must be
connected for this test to pass
properly.
TEST #5 Internal Reference Checks the internal reference on
the meter.
TEST #6 Ground Reference Checks the meter ground.
Troubleshooting 67
Benchtop pH/ISE Meter Instruction Manual
68 Troubleshooting
Benchtop pH/ISE Meter Instruction Manual
The Operator Assistance Codes E-20 through E-32 alert the user to a
potential problem while calibrating or measuring. Several steps can be
taken to eliminate the problem in each case. If the error code persists after
trying the suggested remedies, contact Thermo Orions Technical
Service Group.
Troubleshooting 69
Benchtop pH/ISE Meter Instruction Manual
70 Troubleshooting
Benchtop pH/ISE Meter Instruction Manual
Error
Code Problem Probable Remedy
E-22 Calibration The millivolts being measured are
Std Error the same for two different standards.
Check that two different standards
or buffers are being used and that
the correct one is being measured.
1. Make fresh standards or buffers
and repeat the last calibration
point or the entire calibration.
Troubleshooting 71
Benchtop pH/ISE Meter Instruction Manual
Error
Code Problem Probable Remedy
E-27 Data Transfer Error There is a problem in transferring
data from the datalogger to a printer
or computer.
1. Press any key to acknowledge
the error.
2. Check to make sure the printer or
other device is securely plugged
into the RS232 port on the meter.
3. Check to make sure your printer
or computer is on and ready to
receive information from
the meter.
4. Check your cable to make sure it
matches the pin configuration of
the meter. Use Thermo Orion Cat.
No. 0ACBL0 for IBM PC or
compatible devices.
72 Troubleshooting
Benchtop pH/ISE Meter Instruction Manual
Error
Code Problem Probable Remedy
4. Make sure electrode(s) are
properly identified, connected
and filled.
5. If error persists, measure a
standard of known concentration
with the same background as the
sample to be measured. Adjust
the standard concentrations and
volumes until results
are obtained.
6. If the sample contains
complexing agents, add an excess
of the agent prior to analysis.
Troubleshooting 73
Benchtop pH/ISE Meter Instruction Manual
Error
Code Problem Probable Remedy
E-32 Blank Error. The meter is unable to calculate a
Models 710Aplus, blank value. This error occurs
720Aplus, during a three or more point
and 920Aplus calibration, when one solution has
been defined as zero concentration
and the actual concentration of the
blank is substantially greater than the
least concentrated standard. This can
also occur during a one or two point
calibration if one of the standards
is defined as zero.
1. Check to make sure electrode(s)
were placed in the proper
standards and blank solution
and recalibrate.
2. Repeat calibration using fresh
blank and standards.
3. Recalibrate using two standards
of known concentration other
than zero.
74 Troubleshooting
Benchtop pH/ISE Meter Instruction Manual
Troubleshooting 75
Benchtop pH/ISE Meter Instruction Manual
76 Troubleshooting
Benchtop pH/ISE Meter Instruction Manual
Chapter XI
Service and Repair
A Return Authorization Number must be obtained from Thermo Orion
Technical Service before returning any product for in-warranty or out-of-
warranty repair, replacement, or credit.
Outside of North America consult your authorized Thermo Orion dealer,
or:
Thermo Orion
500 Cummings Center
Beverly, MA 01915-6199 USA
Tel: 978-232-6000
Dom. Fax: 978-232-6015
Intl. Fax: 978-232-6031
Chapter XII
Notice of Compliance
This equipment generates, uses, and can radiate radio frequency energy and
if not installed and used in accordance with the instruction manual, may
cause interference to radio communications. It has been tested and found to
comply with the limits for a Class A computing device pursuant to Subpart
J of Part 15 of FCC Rules, which are designed to provide reasonable
protection against such interference when operated in a commercial
environment. Operation of this equipment in a residential area is likely to
cause interference in which case the user, at his own expense, will be
required to take whatever measures may be required to correct the
interference.
This digital apparatus does not exceed the (Class A) limits for radio noise
emissions from digital apparatus set out in the Radio Interference
Regulations of the Canadian Department of Communications.
Le prsent appareil numrique n met pas de bruits radiolectriques
dpassant les limites applicables aux appareils numriques (de la class A)
prescrites dans le Rglement sur le brouillage radiolectrique dict par le
ministre des Communications du Canada.
78 Notice of Compliance
Benchtop pH/ISE Meter Instruction Manual
Declaration of Conformity
Thermo Orion
Manufacturer:
Thermo Orion
500 Cummings Center
Beverly, MA 01915 U.S.A.
hereby declares that the products
Benchtop Model 920Aplus
720Aplus
710Aplus
525Aplus
520Aplus
420Aplus
410Aplus
conforms with the following standards and documents
Safety: EC Direcive 72/23/EEC Low Voltage Directive
DIN VDE 0700 Transformers
EN 61010-1: 1990 Laboratory Equipment
UL 1310 Class 2 Power Units
CSA C22.2 No. 223 Extra-Low
Voltage Transformers.
CSA C22.2 No. 151-M1986 Laboratory Equipment
UL Std. No. 1262 Laboratory Equipment
80 Certificate of Conformity
Benchtop pH/ISE Meter Instruction Manual
Chapter XIII
Instrument Warranty
The Thermo Orion warranty covers failures due to manufacturers
workmanship or material defects from the date of purchase by the user.
User should return the warranty card to Thermo Orion and retain proof
of purchase. Warranty is void if product has been abused, misused, or
repairs attempted by unauthorized persons.
In the event of failure within the warranty period, Thermo Orion will at
Thermo Orions option, repair or replace product not conforming to this
warranty. There may be additional charges, including freight, for
warranty service performed in some countries. For service, call Thermo
Orion (or its authorized dealer outside the United States and Canada).
Thermo Orion reserves the right to ask for proof of purchase, such as the
original invoice or packing slip.
Instrument Warranty 81
Benchtop pH/ISE Meter Instruction Manual
The warranty period for 960 Titrator PLUS, 950 Fast QC Titrator, Wine
Master and 930 Ionalyzer pumps is three (3) months from date of
purchase.
82 Instrument Warranty
Benchtop pH/ISE Meter Instruction Manual
workmanship for a period of six (6) months from date of purchase by the
customer or twelve (12) months from date of shipment from Thermo
Orion, whichever is earlier when used in accordance with the operating
limitations and maintenance procedure in the instruction manual and
when not having been subjected to accident, alteration, misuse or abuse.
Waterproof meters (Models 630, 635, 830A, 835A, 260A, 261S, 265A,
266S, 130A, 131S, 135A, 136S, 1230, 142 and 842), Conductivity
meters (Models 105Aplus, 115Aplus, 125Aplus, 145Aplus, 150Aplus
and 162A), pHuture MMS meters (Models 535A, 555A),
pH/Conductivity meter (Model 550A), Dissolved Oxygen meters
(Models 810Aplus, 850Aplus and 862A), are warranted to be free from
defects in material and workmanship for a period of thirty-six (36)
months from the date of purchase by the user or forty-two (42) months
from date of shipment from Thermo Orion, whichever is earlier, provided
use is in accordance with the operating limitations and maintenance
procedures in the instruction manual and when not having been subjected
to accident, alteration, misuse or abuse.
Instrument Warranty 83
Benchtop pH/ISE Meter Instruction Manual
84 Instrument Warranty
Benchtop pH/ISE Meter Instruction Manual
Chapter XIV
Ordering Information
Thermo Orion
Cat. No. Description
0410A0 Model 410Aplus pH/mV/Temperature Meter, 110V.
Includes Thermo Orion Sure-Flow Combination pH probe.
0410A1 Model 410Aplus pH/mV/Temperature Meter, 220V.
Includes Thermo Orion Sure-Flow Combination pH probe.
0420A0 Model 420Aplus pH/mV/Temperature Meter, 110V.
Includes Thermo Orion Triode pH/ATC probe.
0420A1 Model 420Aplus pH/mV/Temperature Meter, 220V.
Includes Thermo Orion Triode pH/ATC probe.
0520A0 Model 520Aplus pH/mV/Temperature Meter with
custom vacuum fluorescent display, 110V. Includes
Thermo Orion combination ROSS electrode, 8102BN.
0520A1 Model 520Aplus pH/mV/Temperature Meter with
custom vacuum fluorescent display, 220V. Includes
Thermo Orion combination ROSS electrode, 8102BN.
0520A3 Model 520Aplus Benchtop pH/mV/Temperature
Meter includes Thermo Orion Triode 3 in 1 combination
pH/ATC probe, 110V wall adapter, benchtop meter
starter kit (OBCHSK).
0525A0 Model 525Aplus pH/mV/Temperature Meter with
custom vacuum fluorescent display, 110V. Includes
Thermo Orion combination ROSS electrode, 8156BN.
0525A1 Model 525Aplus pH/mV/Temperature Meter with
custom vacuum fluorescent display, 220V. Includes
Thermo Orion combination ROSS electrode, 8156BN.
0525A3 Model 525Aplus Benchtop pH/mV/Temperature
Meter includes Thermo Orion Triode 3 in 1 combination
pH/ATC probe, 110V wall adapter, benchtop meter
starter kit (OBCHSK).
0710A1 Model 710Aplus Portable pH/ISE/mV/Temperature
Meter, 110V. Includes Thermo Orion combination ROSS
electrode, 8102BN.
0720A0 Model 720Aplus pH/ISE/mV/Temperature Meter
with custom vacuum fluorescent display, 110V.
Includes Thermo Orion combination ROSS
electrode, 8102BN.
0720A1 Model 720Aplus pH/ISE/mV/Temperature Meter
with custom vacuum fluorescent display, 220V.
Includes Thermo Orion combination ROSS
electrode, 8102BN.
Ordering Information 85
Benchtop pH/ISE Meter Instruction Manual
Thermo Orion
Cat. No. Description
0720A3 Model 720Aplus pH/ISE/mV/Temperature Meter
includes electrode holder and stand, 110V
wall adapter.
0920A0 Model 920Aplus pH/ISE/mV/Temperature Meter with
incremental techniques and custom vacuum
fluorescent display, 110V. Includes Thermo Orion
combination ROSS electrode, 8102BN.
0920A1 Model 920Aplus pH/ISE/mV/Temperature Meter with
incremental techniques and custom vacuum
fluorescent display, 220V. Includes Thermo Orion
combination ROSS electrode, 8102BN.
0920A3 Model 920Aplus pH/ISE/mV/Temperature Meter,
includes electrode holder and stand, 110v wall
adapter and instruction manual.
0BCHSK Benchtop Meter starter kit, includes electrode holder
and stand, ATC probe, one 475 mL bottle each of pH
4, pH 7, and pH 10 buffer solution and one 475 mL
bottle of electrode storage solution.
0900A0 Portable Printer for Thermo Orion Meters. Includes
Rechargeable battery and one roll of printer paper. 110V.
0900A1 Portable Printer for Thermo Orion Meters. Same
as above except 220V. DTCL20 DataCOLLECT
for window
0ACBL0 RS232 Interface Cable for use with computers and
other RS232 devices. IBM PC or compatible device.
DTCL20 DataCollect for Windows
090070 Electrode Holder and Stand.
9157BN Thermo Orion Triode Combination pH/ATC Probe for
Thermo Orion A Series Meters.
9107BN Thermo Orion Low Maintenance Triode Combination
pH/ATC probe for Thermo Orion A Series Meters.
917005 Epoxy ATC Probe for Thermo Orion A Series Meters.
917006 Glass ATC Probe for Thermo Orion A Series Meters.
8102BN Ross Combination Glass pH Electrode.
8156BN ROSS Combination Epoxy pH Electrode.
8172BN Ross SURE-FLOW Combination Glass pH Electrode.
8165BN Ross SURE-FLOW Epoxy-Body Combination
pH Electrode.
86 Ordering Information
Benchtop pH/ISE Meter Instruction Manual
Thermo Orion
Cat. No. Description
8175BN Ross SURE-FLOW Semi-Micro Epoxy-Body
Combination pH Electrode
020125 Line Converter, 110V.
020130 Line Converter, 220V.
020135 Line Converter, 240V
910104 pH 4 Buffer Solution, 475 mL
910105 pH 5 Buffer Solution, 475 mL
910107 pH 7 Buffer Solution, 475 mL
910110 pH 10 Buffer Solution, 475 mL
910410 perpHectBuffer Solution pH 4, 10 pk
910425 perpHect Buffer Solution pH 4, 25 pk
910710 perpHect Buffer Solution pH 7, 10 pk
910725 perpHect Buffer Solution pH 7, 25 pk
910110 perpHect Buffer Solution pH 10, 10 pk
910125 perpHect Buffer Solution pH 10, 25 pk
911110 perpHect Electrode Rinse , 10 pk
911125 perpHect Electrode Rinse , 25 pk
910001 pH Electrode Storage Solution, 475 mL
Ordering Information 87
Benchtop pH/ISE Meter Instruction Manual
88 Ordering Information
Benchtop pH/ISE Meter Instruction Manual
Appendix A
Overview of Meter Functions
Model 410Aplus
SETUP CALIBRATE MEASURE
ready
ON C timer
OFF ATC
4 7 10 Man
SETUP:
S-1 RESOLUTION mode
S-2 SLOPE
S-3 PRINT OPTIONS
S-4 CAL BUFFER OPTION
yes
ERROR CODES:
E-20 OUT OF RANGE
E-21 AUTO BUFFER ERROR
E-22 CAL STANDARD ERROR no
print
E-23 BAD SLOPE
E-29 PRINTER ERROR
E-31 BAD VALUE
power
See Manual for other Error Codes.
AUTOSHUTOFF
To turn the AUTOSHUTOFF feature off, press and hold mode during
power up of the meter. After a power interruption or failure, this procedure
must be repeated.
Appendix B
Overview of Meter Functions
Model 420Aplus, 710Aplus
SETUP CALIBRATE MEASURE
2nd
ready
bold
EXP
ON C
OFF ATC
pH mV Rel mV Conc
SETUP:
yes no
1-1 READY
1-2 HOLD
1-3 BEEP
1-4 AUTOSHUTOFF
2-1 SLOPE
2-2 RESOLUTION measure
2-3 ISOPOINT timer print
2-4 RESET
3-1 TIMER INTERVAL
3-2 REMAINING TIME
3-3 SET REAL TIME
3-4 SET DATE
4-H HIGH ALARM LIMIT
mode
4-L LOW ALARM LIMIT setup cal
5-1 PRINT MODE: 1 = MAN, 2 = READY, 3 = TIMED
5-2 PRINT INTERVAL
6-1 DATA LOG MODE: 1 = MAN, 2 = READY, 3 = TIMED, 0 = OFF
6-2 DATA LOG INTERVAL
6-3 DOWNLOAD DATA
6-4 DATA CLEAR power 2nd
See Manual for Error Codes.
mode Press to change operating modes. The options are pH, mV, or
REL mV. (conc with 710Aplus Meter only)
timer Press to start the timer. When the preset time has
elapsed the instrument will beep for 1 minute (or
until a key is pressed).
3-3 SET REAL Set the actual time of day. The meter TIME
uses a 24 hour clock. When the code 3-3
is displayed in the lower field, the current
time (hours:minutes) is displayed in the main
field. If correct press yes, otherwise change
as required and then press yes.
3-4 SET DATE Sets the current date. When the code 3-4 is
displayed in the lower field, the current date
(month: day) is displayed in the main field.
Press yes, or change the date as needed, then
press yes. Next the current year is displayed.
Press yes or change as required, then
press yes.
4-1 HIGH Sets the high alarm limit for the current
ALARM measurement mode. When the reading
LIMIT exceeds the set limit the alarm will
(710Aplus only) ring. The maximum limits are 19.999 for
pH, 19900 for concentration, +1600.0 for
mV, and +1999.9 for relative millivolts.
Press or to change, then press yes.
4-2 LOW Sets the low alarm limit for the current
ALARM measurement mode. When the reading falls
LIMIT below the set limit the alarm will ring.
(710Aplus only) The minimum limits are -2.000 pH, 0.0
for Concentration, -1600.0 for mV and
-1999.9 for relative millivolts. Press or
to change, then press yes.
5-2 SET PRINT Sets the timed print interval. The maximum
print INTERVAL is 23 hours, 59 minutes,
and 59 seconds. The minimum print interval
is 5 seconds. When the code 5-2 is displayed
in the lower field, the current print interval
hours will flash in the main display.
Press yes or press or to change, then
press yes. For the print interval minutes:
seconds will be displayed in the main field.
Press yes or change, then press yes. The
default setting is 1 minute.
Appendix C
Overview of Meter Functions
Models 520Aplus, 525Aplus, 720Aplus, and 920Aplus
1 2 3
yes mode calibrate
cal edit
measure
stability
4 5 6
no hold
incr tech
resolution
channel
slope
electrode
ID
7 8 9
1st print
data log
activate
set timer
set temp
ready
mV
2nd
+-
date/time
0
standby
.
data/print
beep options modes
alarm
model 920
Key 1 Mode
Pressing mode (1) changes the instrument operating mode. The current
operating mode designation is displayed to the right of the main display.
Key 2 Calibrate
Pressing calibrate (2) starts a calibration. The time and date of the last
calibration are displayed, then the instrument starts the calibration
sequence. See the individual meter sections for instructions on calibration
and measurement.
Cal Edit (920Aplus only)
Pressing 2nd then cal edit(2) allows you to change or delete a calibration
point after the calibration has been completed. This is useful for multipoint
calibrations. See the Model 920Aplus (special features) section for more
information.
Key 3 Measure
Pressing measure (3) returns the meter to measure mode for sample
analysis. While in measure mode, the sample temperature, ready, and hold
are displayed. The Model 525Aplus, 720Aplus and 920Aplus include
channel designation. The 920Aplus also displays the electrode I.D.
When using hold, pressing measure will unlock hold for additional
sample analysis.
Stability (920Aplus only)
Pressing 2nd then stability (3) accesses the stability function on the
920Aplus. The main readout will display the absolute drift rate of the
electrode in mV/minute. The prompt line displays mV/min and a graphic
depiction of stability in the form of a bar. As the electrode becomes more
stable, more of the bar will be filled in. When the electrode reaches stability
READY replaces mV/min and the bar is completely filled.
Key 4 Hold
Pressing hold (4) acts as a toggle changing the hold setting. This function
freezes the display when the electrode signal is stable. The prompt line
verifies your selection then returns to the measure mode.
Example: HOLD ON.
When hold is turned on, the ready mode is automatically turned on. When
the display is frozen, press measure to reactivate the display and analyze
another sample.
The hold designation appears as HOLD (520Aplus) or HLD (525Aplus,
720Aplus and 920Aplus) in the prompt line and replaces the RDY when it
is displayed on the 920Aplus.
Incremental Techniques (920Aplus only)
Pressing 2nd then inc tech (4) accesses incremental techniques on the
920Aplus. Incremental techniques are additional measurement techniques
for analysis with ion selective electrodes. Both Single and Double Known
Addition and Known Subtraction can be performed using this function. See
the 920Aplus (special features) section for a complete description.
Key 5 Resolution
Press resolution (5) for selection of resolution in pH mode. Up to three
places beyond the decimal may be selected. For example, the pH display
will change from 7.000 to 7.0 to 7.00 to 7.000 with each keypress.
On the 720Aplus and 920Aplus, press resolution (5) for resolution
selection in concentration mode. Select up to three significant digits. For
example, the display will change as follows: 1.45 to 1 to 1.5 to 1.45.
The lower the resolution selected, the faster the ready indicator will be
displayed. The choice of greater accuracy, i.e. higher resolution, or greater
speed, is up to the user and may be changed to suit the analysis
being performed.
It is recommended that the display resolution be selected prior to
calibration and measurement. Calibration and measurement should take
place at the same resolution. Calibrating at one resolution, then selecting a
higher resolution for measurement is not recommended.
Key 8 Activate
Press activate (8) to start the timer interval. An audible signal will sound at
the completion of the timer interval.
Activate Timer
Once the timer interval has been set, it is easily activated by pressing
activate (8). The meter verifies the action with the following prompts.
Prompt Keypress/Action
Press Activate (8)
1. Activate Timer? Press yes
2. TIMER ON Meter returns to measure mode.
3. Time remaining Displayed when the timer has been
turn off? previously activated but has not yet
completed the interval. The meter
displays the actual time in the main display.
Press yes.
4. Timer off
6. Timer on Press no to continue with the current
interval and return to measure mode.
Set Timer
To set the timer interval press 2nd then set timer (8). The meter will
present a series of prompts as follows:
Prompt Keypress/Action
Timer interval Press 2nd then set timer (8).
Press yes to accept the hours setting, or
1. Timer Interval hour change as desired.
2. Timer interval Minute Enter the correct setting and press yes.
3. Timer interval second Enter the correct setting using the
numeric keys then press yes.
4. Timer interval The meter displays the new timer
interval in the main display then
returns to measure mode.
Beep
Press 2nd then Beep to turn the beep on or off. Turning the beep on will
cause the meter to beep when a key is pressed, when an operator assistance
code occurs, and when the electrode signal becomes stable (if ready is on).
Turning the beep off disables the audible feedback function except when
the alarm or timer is set.
Alarm
The Models 520Aplus, 525Aplus, 720Aplus, and 920Aplus have an alarm
function. Separate alarms can be set for each mode and for channels 1 and 2
(on the 525Aplus, 720Aplus and 920Aplus). The alarm limits can be set at
the limits of each measuring range, -2.000 to +19.999 pH, -1600.0 to
+1600.0 mV, -1999.9 to +1999.9 relative mV, and 0.0 to 19900 for
concentration (720Aplus and 920Aplus only).Press 2nd then alarm to
access the set alarm function, then follow the prompts.
Prompt Keypress/Action
Press 2nd then alarm.
1. 1-PH LIMIT HI The current high alarm limit for
MEASUREMENT the mode and channel is displayed.
Change as desired then press yes.
2. 1-PH LIMIT LO The current low alarm limit for the
MEASUREMENT mode is displayed. Change as
desired, then press yes. Meter then
returns to MEASURE mode.
Repeat sequence for each channel. Select the other channel by pressing
2nd, then channel, then set alarms.
NOTE: The prompts above are for the
Model 920Aplus.
Key 0 Standby
Press standby (0) to place meter into standby mode. When in standby
mode, the display of the meter is illuminated at half intensity. This feature
prolongs display life. The prompt is STANDBY: ANY KEY. Press any key
to restart meter operation. See Autoshutoff section to take advantage of
another meter saving feature.
Options
Press 2nd then options (0) to change the isopotential point, clear or transfer
data from the datatlogger, select the autoshutoff feature, or identify the use
of a switchbox (920Aplus only) The menu loop is as follows:
OPTIONS MENU
CHANGE ISO PT?
TRANSFER DATA?
DATA CLEAR?
AUTOSHUTOFF ON
SWITCHBOX IN USE?
See details below on each function.
Changing the Isopotential Point
The isopotential point may be changed for pH electrodes on all meters and
ISE electrodes on the 920Aplus. For pH the default value is 7.000. For
ISEs the default value is - - - (No ISO). Identifying an isopotential point
for an ISE electrode will provide temperature compensated concentration
measurements when using the direct measurement technique.
To change an isopotential point follow the keypress/prompt sequence
below. Always recalibrate after changing an isopotential point.
NOTE: The CHANGE ISO PT option can only be
accessed while in pH or concentration mode. If meter is
in mV or RmV mode TRANSFER DATA will be the first
option displayed.
First make sure your electrode I.D.(920Aplus) and channel selection
(920Aplus, 525Aplus, and 720Aplus) are correct.
Prompt Keypress/Action
Press 2nd then options.
1. OPTIONS MENU Press yes.
CHANGE ISO PT.?
2. ISO-ENTER VALUE Enter the isopotential point value for the
electrode then press yes.
3. ISO EQUALS 7.000 The isopotential point is verified then the
meter proceeds to the next item in the
options menu.
4. TRANSFER DATA Press 1st then calibrate to exit and start the
calibration or 1st then measure to exit to
measure mode.
Transfer Data
To transfer data from the internal datalogger to a printer or computer press
yes in response to the TRANSFER DATA? prompt. While the data is being
transferred the prompt line will display DATA TRANSFER. On completion
the DATA TRANSFERRED prompt will be displayed followed by the next
option in the menu.
Data Clear
Transferring data from the datalogger does not clear the memory. Pressing
yes to the DATA CLEAR? prompt will clear the datalogger memory. DATA
CLEARING followed by DATA CLEARED will be displayed. The meter
then displays the next option in the menu, AUTOSHUTOFF.
NOTE: Prior to using the datalogger for the very
first time, it will be necessary to clear datalogger
memory with the DATA CLEAR option.
Autoshutoff Feature
The meters have a special feature that will help to prolong meter life called
autoshutoff. When autoshutoff has been activated the meter will enter
standby mode if no keys have been pressed for ten minutes. To start the
meter again, press any key.
Prompt Keypress/Action
Press 2nd then Options.
1. OPTIONS MENU Press no.
CHANGE ISO PT?
2. TRANSFER DATA? Press no.
3. DATA CLEAR? Press no
4. AUTOSHUTOFF ON Press yes.
5. AUTOSHUTOFF OFF The meter verifies your
selection then proceeds to the
next item in the options menu.
(The 520Aplus, 525Aplus, and
720Aplus return to
measure mode).
Appendix D
Meter Default
pH electrode slope 100.0%
pH isopotential point 7.000
pH resolution .001
temperature 25.0oC
ready on
hold off
beep on
autoshutoff on
timer interval 5 seconds
print mode 1-manual print
print interval 1 minute
datalog mode 0-off (520Aplus, 525Aplus, 710Aplus,
720Aplus, and 920Aplus)
measurement channel Channel 1 (525Aplus, 720Aplus and 920Aplus)
electrode ID pH (920Aplus)
high alarm limit off (520Aplus, 525Aplus, 710Aplus, 720Aplus
and 920Aplus)
low alarm limit off (520Aplus, 525Aplus, 710Aplus, 720Aplus
and 920Aplus)
Appendix E
Multipoint Calibration Curve
Models 410Aplus, 420Aplus, 520Aplus, 525Aplus, 710Aplus, 720Aplus,
and 920Aplus utilize a point-to-point scheme for multipoint calibrations
in both pH and CONCENTRATION modes. In this technique, straight line
segments are drawn between each successive pair of calibration points.
Separate slope and Eo values are calculated using the adjacent pair of data
points. For example, in a five point calibration, four slopes and five Eos are
calculated to correspond to the four line segments that connect the data.
Concentration or pH values for samples are calculated according to which
segment their potential values fall into. Those above or below the range of
calibration are calculated by extrapolation from the top or bottom most
segment.
The displayed slope is an average slope of the separate segments. The slope
may be outside the range normally expected for an electrode. For example,
if the slope of one segment is low, then the average will be lower than
theoretical, even though the response may be Nernstian over most of
the range.
See Figure below for an example of a typical calibration curves using
this method.
250
200
150
100
50
0
-50
-100
-150
-200
-250
mV
Segmented Calibration ( )
and Best Fit Line ( )
Appendix F
Automatic Blank Correction
The Models 710Aplus, 720Aplus, and 920Aplus have a feature known as
automatic blank correction. In this technique the meter decides whether
blank correction is the best measurement strategy by analyzing the
electrode response during a multipoint calibration. A separate blank does
not have to be run. Graphically, automatic blank correction is equivalent to
drawing a smooth curve through the lowest three points of the multipoint
calibration and extrapolating to zero concentration based on the assumption
of Nernstian electrode behavior.
Multipoint calibrations at the lower limit of detection are desirable when
the response of an ion selective electrode is non-linear and cannot be
characterized with a one or two point calibration. This is usually seen as a
low electrode slope. Generally the electrode is behaving in a Nernstian
manner but the effect of a blank is being observed. See Figure below.
This blank may be a true reagent blank, traces of analyte ion in the
reagents, or it may be the mud value of the electrode. It could also be an
interferent in the reagents that becomes apparent at low levels of analyte
ion, or it could be any combination of these effects.
The expanded version of the Nernst equation traditionally used for blank
correction is as follows:
E = Eo + S x log(C + b) where b is the blank.
In a multi-point calibration a set of equations is generated and the
relationship between them evaluated.
For example the equations generated in a three point calibration would be
as follows:
E1 = Eo + S x log (C1 + b )
E2 = Eo + S x log (C2 + b )
E3 = Eo + S x log (C3 + b )
The meter evaluates the relationships between the three potentials E1, E2,
and E3 and the three concentrations C1, C2, and C3. If the relationships
indicate that blank correction is desired, a blank will automatically be
calculated, and the non-linearity will be corrected for in a Nernstian
manner. If the appropriate conditions are not met, the blank is set at zero
and each segment of the multipoint calibration is treated independently.
When all three of the following conditions are met, blank correction
is invoked.
1. The concentration of the first standard is zero, or the slope of the
electrode between the first and second standards is less than the slope
between the second and third.
2. Potential differences between points are significant. For example:
E3 - E1 > 10 mV
3. The blank correction algorithm converges at reasonable blank and
slope values. Conditions in steps 1 and 2 prevent failure to converge in
most situations. However, the slope will attain any value necessary for
convergence to a calculated blank value of 3 x C3.
When blank correction is implemented, the slope value for the electrode
may be outside the range of values normally considered acceptable during
an ordinary calibration. For best results calibration standards should be
close in range to the expected sample concentrations and should bracket
the expected sample concentration.
When conditions 1, 2, and 3 above are not met, the calibration data is
handled by the multipoint calibration method described earlier.
NOTE: When a calibration standard is defined as zero
and the meter cannot calculate a blank, the operator
assistance code E-32 will be displayed. See
Troubleshooting section.
In calibrations with more than three points, a combination of methods are
utilized. Automatic blank correction is used if the lowest three points
satisfy the criteria and multipoint calibration is used for the other points.
The slope displayed after calibration is an average of the slope calculated
in the automatic blank correction algorithm and the slope(s) for each
additional segment.
Appendix G
Determining Isopotential Points
To take advantage of the temperature compensation feature for
concentration measurements in the Model 920Aplus, it is necessary to
experimentally determine the isopotential point of the electrode in use.
The slope of all electrodes changes with temperature. This is due in fact to
the slope term, S, in the Nernst equation. The slope is actually 2.3RT/nF,
where R and F are constants, n is the charge on the measured species, and
T is the temperature in degrees Kelvin. Examples of calibration curves at
varying temperatures are shown in Figure below.
These curves intersect at the isopotential point, which is the concentration
at which the potential of the electrode does not vary with temperature. If
the isopotential point is known or can be measured experimentally, and if
the meter, such as the Model 920Aplus, has the means of adjusting the
isopotential point, temperature compensation for an ion selective electrode
is possible. Temperature compensation can be performed for one and two
point calibrations.
-100
-80
-60
isopotential point
(5 x 10 -3 M)
-40
-20
10-3 10-2
sodium concentration,
Sodium moles
Concentration (moles per liter
per liter)
concentration
range NA NA NA NA
resolution NA NA NA NA
relative accuracy NA NA NA NA
relative millivolt
range NA -1999.9 to +1999.9 -1999.9 to +1999.9 -1999.9 to +1999.9
inputs One BNC, One pin tip, One BNC, One pin tip, One BNC, One pin tip, Two BNC, Two pin tip,
ATC, power, RS232 ATC, power ATC, power, RS232, Karl Fischer ATC, power, RS232, Karl Fischer
power 9 VDC line converter 9 VDC line converter 9 VDC line converter 9 VDC line converter
requirements 110 or 220 VAC. 110 or 220 VAC. for either 110 or 220 VAC. for either 110 or 220 VAC,
50/60 Hz walloutlet. 50/60 Hz walloutlet. 50/60 Hz wall outlet. 50/60 Hz wall outlet.
impedance
instrument drift < 50 microvolts/oC < 50 microvolts/oC < 50 microvolts/oC < 50 microvolts/oC
input bias current < 1 picoamp at 25oC and < 4 picoamps over full operating range
Specifications 125
SPECIFICATIONS
710Aplus 720Aplus 920Aplus
pH range -2.000 to 19.999 -2.000 to 19.999 -2.000 to 19.999
concentration
range 0.000 to 19900 0.000 to 19900 0.000 to 19900
resolution one least significant digit one least significant digit one least significant digit
relative accuracy 0.05% of reading 0.5% of reading 0.5% of reading
relative millivolt
range -1999.9 to +1999.9 -1999.9 to +1999.9 -1999.9 to+1999.9
inputs two BNC, two pin tip two BNC, two pin tip two BNC, two pin tip
ATC, power, RS232 ATC, power, RS232, Karl Fischer ATC, power, Karl Fischer
power 9 VDC line converter for either 110 9 VDC line converter for either 110 9 VDC line converter for either 110
requirements or 220 VAC. 50/60 Hz walloutlet. or 220 VAC. 50/60 Hz wall outlet. or 2209 VAC. 50/60 Hz waloutlet.
input 12
>10 ohms
12
>10 ohms
12
>10 ohms
impedance
instrument drift < 50 microvolts/oC < 50 microvolts/oC < 50 microvolts/oC
input bias current < 1 picoamp at 25oC and < 4 picoamps over full operating range
126 Specifications
AQUAfast, Cahn, EZFlash, Ionalyzer, ionplus, KNIpHE, ORION, perpHect, PerpHecT, PerpHecTion, pHISA, pHix, pHuture, Pure Water, Sage, Sensing the Future, SensorLink, Sure-Flow,
Titrator PLUS, TURBO2 and Wine Master are registered trademarks of Thermo Orion.
1-888-pHAX-ION, A+, All in One, Aplus, AQUAsnap, AssuredAccuracy, AUTO-BAR, AUTOCAL, AUTO DISPENSER, Auto-ID, AUTO-LOG, AUTO-STIR, AUTO-READ, BOD AutoEZ,
Cable-Free, CERTI-CAL, CISA, DataCOLLECT, DataPLUS, digital LogR, DirectCal, DuraProbe, Environmental Product Authority, Extra Easy/Extra Value, FAST QC, Flash GC, GAP, GLPcal,
GLPcheck, GLPdoc, KAP, LabConnect, LogR, Minimum Stir Requirement, MSR, NISS, One-Touch, One-Touch Calibration, One-Touch Measurement, Optimum Results, Pentrode, pHuture
MMS, pHuture Pentrode, pHuture Quatrode, pHuture Triode, Quatrode, QuiKcheK, rf link, ROSS, ROSS Resolution, SAOB, Stacked, Stat Face, The Enhanced Lab, ThermaSense, Triode,
TRIUMpH, Unbreakable pH, Universal Access are trademarks of Thermo Orion.
Guaranteed Success and The Technical Edge are service marks of Thermo Orion.
ORION Series A meters and 900A printer are protected by U.S. patents 5,108,578, 5,198,093, D334,208 and D346,753.
ORION 81, 82, 91, and 92 series glass electrodes are protected by U.S. patents 4,661,236 and 4,687,500.
Sure-Flow electrodes are protected by European Patent 278,979 and Canadian Patent 1,286,720.
The specifications, descriptions, drawings, ordering information and part numbers within this document are subject to change without notice.
233541-001 Rev. B