Bacillus thuringiensis is used to control

Nematodes
Fungal pathogens
Bacterial pathogens
Insect pests
Question 2
WRONG
The genetically-modified (GM) brinjal in India has been developed for
Enhancing shelf life
Insect-resistance
Drought-resistance
Enhancing mineral content
Question 3
WRONG
The protein products of the following Bt toxin genes cry IA c and cry II Ab are
responsible for controlling
Bollworm
Roundworm
Moth
Fruit fly
Question 4
WRONG
Variable number of tandem repeats (VNTRs) in the DNA molecule are highly useful in
Monoclonal antibody production
DNA fingerprinting
Recombinant DNA technology
Stemcell culture
Question 5
WRONG
Silencing of mRNA has been used in producing transgenic plants resistant to
Bollworms
Bacterial blights
White rusts
Nematodes
Question 6
WRONG
Restriction endonucleases are most widely used in recombinant DNA technology.
They are obtained from
Bacteriophages
Bacterial cells
 Plasmids
All prokaryotic cells
Question 7
WRONG
PCR proceeds in three distinct steps governed by temperature, they are in order of
Annealing, Synthesis, Denaturation
Synthesis, Annealing, Denaturation
Denaturation, Annealing, Synthesis
Denaturation, Synthesis, Annealing
Question 8
WRONG
Which of the following is correctly matched?
Ligase - Molecular scissors
Thermus aquaticus - Bt-gene
Agrobacterium tumefaciens - Tumour
Hind II - Plasmid vector
Question 9
WRONG
Sterilization of tissue culture medium is done by
mixing the medium with antifungal agents
filtering the medium through fine sieve
autoclaving of medium at 120 for 15 min
keeping the medium at 20C
Question 10
WRONG
Biolistics (gene-gun) is suitable for
Constructing recombinant DNA by joining with
vectors
Transformation of plant cells
Disarming pathogen vectors
DNA finger printing
Question 11
WRONG
An improved variety of transgenic basmati rice
Gives high yield and is rich in vitamin A
Does not require chemical fertilizers and growth
hormones
Give high yield but has no characteristic aroma
Is completely resistant to all insect pests and
diseases of paddy
Question 12
WRONG
In cloning of cattle a fertilized egg is taken out of the mother's womb and
in the eight cell stage the individual cells are
separated under electrical field for further
development in culture media
the egg is divided into 4 pairs of cells which are
implanted into the womb of other cows
in the eight cell stage, cells are separated and
cultured until small embryos are formed which are
implanted into the womb of other cows.
from this up to eight identical twins can be
produced
Question 13
WRONG
Which one of the following palindromic base sequences in DNA can be easily cut at
about the middle by some particular restriction enzyme?
5 CACGTA 3; 3 CTCAGT 5
5 CGTTCG 3; 3 ATGGTA 5
5 GATATG 3; 3 CTACTA 5
5 GAATTC 3; 3 CTTAAG 5
Question 14
WRONG
Assertion: In recombinant DNA technology, human genes are often transferred into
bacteria (prokaryotes) or yeast (eukaryote).
Reason: Both bacteria and yeast multiply very fast to form huge population, which
express the desired gene.
Both Assertion and Reason are true and the
Reason is the correct explanation of the Assertion.
Both Assertion and Reason are true but the Reason
is not the correct explanation of the Assertion.
Assertion is true statement but Reason is false.
Both Assertion and Reason are false statements.
Question 15
WRONG
Compare the statements A and B.
Statement A: Synthesis of DNA takes place in the S-phase of interphase.
Statement B: Every chromosome, during metaphase, has two chromatids.
Choose the correct description :
Statement A is correct and B is wrong.
Statement A is wrong and B is correct.
 Both the statements A and B are correct and A is
not the reason for B.
Both the statements A and B are correct and A is
the reason for B

A dividing and undifferentiated mass of cells is called

Callus
Embryo
Explant
Zygote
Question 2
WRONG
Advancement in genetic engineering has been possible due to the discovery of
Oncogenes
Transposons
Restriction endonuclease
Exonucleases
Question 3
WRONG
Agarose extracted from sea weeds finds use in
Gel electrophoresis
Spectrophotometry
Tissue culture
PCR
Question 4
WRONG
Continuous addition of sugars in 'fed batch' fermentation is done to
Degrade sewage
Produce methane
Obtain antibiotics
Purify enzymes
Question 5
WRONG
Which purine is found in RNA?
Guanine
Cytosine
Thymine
Uracil
 Question 6
WRONG
DNA fingerprinting was discovered by
James Watson
Alec Jeffreys
Frederick Fanger
Hargobind Khorana
Question 7
WRONG
DNA is found in
Chromosomes
Plastids
Mitochondria
All of the above
Question 8
WRONG
Dolly, The first cloned mammal, was produced by
Parthenogenesis
Artificial fertilization
Replacement of zygote nucleus by somatic nucleus
in vitro fertilization
Question 9
WRONG
Embryo culture is used for
Establishing suspension culture
Recovery of inter specific hybrids
Somatic hybridization
Haploid production
Question 10
WRONG
First artificial gene was synthesized by
Nirenberg
Mendel
Morgan
Khorana
Question 11
WRONG
Genetic Engineering is
plastic surgery
addition or removal of genes
study of extranuclear genes
 all of the above
Question 12
WRONG
Genomics is the study of
Genes in general
Human genes
Genomes in general
Human genome
Question 13
WRONG
Given below is a sample of a portion of DNA strand giving the base sequence on the
opposite strands. What is so special shown in it? 5' ______ GAATTC ________ 3' 3'
________CTTAAG ________ 5'
Palindromic sequence of base pairs
Replication completed
Deletion mutation
Start codon at the 5' end
Question 14
WRONG
Maximum number of existing transgenic animals is of
Pig
Fish
Mice
Cow
Question 15
WRONG
One of the following processes involves southern blotting technique
rDNA technology
DNA finger printing
production of transgenic plants
formation of suspension cultur

Question 1
WRONG
Plant tissue culture technique is a redefined method of ________
Hybridization
Vegetative propagation
 Asexual reproduction
selection
Question 2
WRONG
Polyethylene glycol is
Fusogenic chemical
Electro fusion stimulant
Callus stimulant
Differentiation stimulant
Question 3
WRONG
Restriction endonucleases are used in genetic engineering and they
cut DNA at various sites
join DNA segments
cut RNA at specific sites
cut DNA at specific sites
Question 4
WRONG
Restriction endonucleases are used in genetic engineering, because
they can degrade harmful proteins
they can join DNA fragments
they can cut DNA at specific base sequences
they can cut DNA at variable sites
Question 5
WRONG
Somatic hybridization is achieved through
Grafting
Protoplast fusion
Conjugation
Recombinant DNA technology
Question 6
WRONG
Structure involved in genetic engineering is
Plasmid
Codon
Plastid
Scissors
Question 7
WRONG
The enzymes required to obtain wall-free / naked protoplasts are
Cellulase and proteinase
 Cellulase and pectinase
Cellulase and amylase
Amylase and pectinase
Question 8
WRONG
The first hormone artificially produced by bacteria is
insulin
thyroxin
adrenalin
testosterone
Question 9
WRONG
The first transgenic crop was
Pea
Tobacco
Flax
Cotton
Question 10
WRONG
The most common substrate used in distilleries for the production of ethanol is
Molasses
Corn meal
Soya meal
Ground gram
Question 11
WRONG
The nitrogen base which is absent in RNA is
Adenine
Cytosine
Guanine
Thymine
Question 12
WRONG
The plant 'Brassica napus' has been modified to produce ______.
Vitamin A
hirudin
lysine rich seeds
insulin
Question 13
WRONG
The process of RNA interference has been used in the development of plants
resistant to
Insects
Nematodes
Fungi
Viruses
Question 14
WRONG
The specific triplet of nitrogen bases on the transfer RNA is called
Codon
Anticodon
Genetic code
none of the above
Question 15
WRONG
The term gene was coined by
Mc Clintock
Morgan
Johannsen
De Duve

Question 1
WRONG
The transgenic animals are those which have
foreign DNA in some of their cells
foreign DNA in all of their cells
foreign RNA in all of their cells
Both (A) and (C)
Question 2
WRONG
The two strands of DNA are held together by bonds of
Nitrogen
Oxygen
Hydrogen
Carbon
Question 3
WRONG
There is a restriction endonuclease called EcoRI. What does "co" part in it stand for?
coli
colon
coelom
coenzyme
Question 4
WRONG
Transgenic plants are developed by
Introducing foreign genes
Introducing gene mutations
Deleting certain chromosomes parts
Stopping spindle formation
Question 5
WRONG
Cry genes or Bt genes are obtained from
Cotton pest
Tobacco plant
Bacillus thuringiensis
E - Coli
Question 6
WRONG
Which enzyme is useful in genetic engineering?
DNAase
Amylase
Lipase
Restriction endonuclease
Question 7
WRONG
Which is related to genetic engineering?
Plastid
Plasmid
Heterosis
Mutation
Question 8
WRONG
Which of the following enzyme joints the okazaki fragments?
DNA polymerase
DNA ligase
Helicase
Restriction endonuclease
Question 9
 WRONG
Which of the following features should be present in vector?
It should replicate autonomously
It should have its own origin of replication
It should possess restriction enzyme cleavage sites
All of these
Question 10
WRONG
Which of the following is a genetic vector?
Plasmid
Phage
Cosmid
All of these
Question 11
WRONG
Which of the following is related to genetic engineering?
Mitochondrion
Golgi apparatus
Lysosome
Plasmid
Question 12
WRONG
Which of the following is used in Biowar?
A pathogen
Toxin from a pathogen
A delivery system for the bioweapon agent
All of these
Question 13
WRONG
Which of the following tool makes possible genetic engineering?
Restriction endonuclease
DNA polymerase
Helicase
RNA polymerase
Question 14
WRONG
Which one contains four pyrimidine bases?
GATCAATGC
GCUAGACAA
UAGCGGUAA
TGCCTAACG
 Question 15
WRONG
Which one is found only in RNA?
Cytosine
Adenine
Uracil
Guanine

Question 1
WRONG
Which one is a true statement regarding DNA polymerase used in PCR?
It is isolated from a virus
It remains active at high temperature
It is used to ligate introduced DNA in recipient cells
It serves as a selectable marker
Question 2
WRONG
Which one of the following is a case of wrong matching?
Micropropagation-In vitro production of plants in
large numbers
Callus - Unorganized mass of cells produced in
tissue culture
Somatic hybridization - Fusion of two diverse cells
Vector DNA - Site for t-RNA synthesis
Question 3
WRONG
Which part would be most suitable for raising virus-free plants for
micropropagation?
Meristem
Node
Bark
Vascular tissue
Question 4
WRONG
For transformation, micro-particles coated with DNA to be bombarded with gene
gun are made up of
 Silicon or Platinum
Gold or Tungsten
Silver or Platinum
Platinum or Zinc
Question 5
WRONG
The figure below is the diagrammatic representation of the E.coli vector pBR 322.
Which one of the given options correctly identifies its certain component(s)?

Hind III, EcoRI-selectable markers

ampR, tetR-antibiotic resistance genes
ori-original restriction enzyme
rop-reduced osmotic pressure
Question 6
WRONG
The linking of antibiotic resistance gene with the plasmid vector became possible
with:
Endonucleases
DNA ligase
Exonucleases
DNA polymerase
Question 7
WRONG
In plant biotechnology, PEG is used in
Protoplast fusion
Cell culture preparation
Protoplast isolation
Hardening
Question 8
WRONG
Polyethylene glycol method is used for
 Energy production from sewage
Gene transfer without a vector
Biodiesel production
Seedless fruit production
Question 9
WRONG
Genetic engineering has been successfully used for producing
Transgenic models for studying new treatments
for certain cardiac diseases
Transgenic mice for testing safety of polio vaccine
before use in humans
Animals like bulls for farm work as they have super
power
Transgenic Cow-Rosie which produces high fat milk
for making ghee
Question 10
WRONG
Restriction endonucleases:
Are synthesized by bacteria as part of their
defense mechanism
Are used for in vitro DNA synthesis
Are used in genetic engineering for ligation of two
DNA molecules
Are present in mammalian cells for degradation of
DNA when the cell dies
Question 11
WRONG
Which of the following enzymes are used to join bits of DNA?
DNA polymerase
Primase
Ligase
Endonuclease
Question 12
WRONG
Which one of the following bacteria has found extensive use in genetic engineering
work in plants?
Bacillus coagulans
Xanthomonas citri
Clostridium septicum
Agrobacterium tumefaciens
Question 13
WRONG
Restriction endonuclease
 Restricts the synthesis of DNA inside the nucleus
Cuts the DNA molecule at specific sites
Cuts the DNA molecule randomly
Synthesizes DNA
Question 14
WRONG
Plasmid has been used as vector because :
Both ends show replication
It can move between prokaryotic and eukaryotic
cells
It is circular DNA which have capacity to join to
eukaryotic DNA
It has antibiotic resistance gene
Question 15
WRONG
A genetically engineered micro-organism used successfully in bioremediation of oil
spills is a species of
Trichoderma
Pseudomonas
Bacillus
Xanthomonas

Question 1
WRONG
Which one of the following is used as vector for cloning genes into higher organisms?
Salmonella typhimurium
Retrovirus
Baculovirus
Neurospora crassa
Question 2
WRONG
The vector for T-DNA is
Thermus aquaticus
Salmonella typhimurium
Agrobacterium tumefaciens
Bacillus thuringiensis
Question 3
 WRONG
Cellular totipotency is demonstrated by
All plant cells
Only gymnosperm cells
All eukaryotic cells
Only bacterial cells
Question 4
WRONG
Crop plants grown in monoculture are
Characterized by poor root system
Free from intraspecific competition
Low in yield
Highly prone to pests
Question 5
WRONG
Differentiation of organs and tissues in a developing organism, is associated with
Differential expression of genes
Developmental mutations
Deletion of genes
Lethal mutations
Question 6
WRONG
Meristem culture is practiced in horticulture to get
Slow-growing callus
Somaclonal variation
Haploids
Virus-free plants
Question 7
WRONG
Micropropagation is a technique
for production of true to type plants
for production of haploid plant
for production of Somatic hybrids
for production of Somaclonal plants
Question 8
WRONG
What is true of plasmid?
Part of nuclear chromosome
Contains genes for vital activities
Found in viruses
Widely used in gene transfer
 Question 9
WRONG
Somaclonal variation appears in plants
Transformed by recombinant DNA technology
Growing in polluted soil or water
Exposed to gamma rays
Raised in tissue culture
Question 10
WRONG
Tobacco plants resistant to a nematode have been developed by the introduction of
DNA that produced (in the host cells)
An antifeedant
A particular hormone
Both sense and anti-sense RNA
A toxic protein
Question 11
WRONG
Plasmids are suitable vectors for gene cloning because
these can shuttle between prokaryotic and
eukaryotic cells
these are small circular DNA molecules with their
own replication origin site
these are small circular DNA molecules, which can
integrate with host chromosomal DNA
these often carry antibiotic resistance genes
Question 12
WRONG
Some of the characteristics of Bt cotton are
Medium yield, long fibre and resistance to beetle
pests
Long fibre and resistance to aphids
High yield and resistance to bollworms
High yield and production of toxic protein crystals
which kill dipteran pests
Question 13
WRONG
Cultivation of Bt cotton has been much in the news. The prefix Bt means ______.
produced by biotechnology using restriction
enzymes and ligases
bigger thread variety of cotton with better tensile
strength
barium-treated cotton seeds
 carrying an endotoxin gene from Bacillus
thuringiensis
Question 14
WRONG
Stirred-tank bioreactors have been designed for
Purification of the product
Addition of preservatives to the product
Availability of oxygen throughout the process
Ensuring anaerobic conditions in the culture vessel
Question 15
WRONG
Restriction endonucleases are enzymes which
Recognize a specific nucleotide sequence for
binding of DNA ligase
Make cuts at specific positions within the DNA
molecule
Remove nucleotides from the ends of the DNA
molecule
Restrict the action of the enzyme DNA polymerase

Question 1
WRONG
The first clinical gene therapy was given for treating?
Rheumatoid arthritis
Chicken pox
Diabetes mellitus
Adenosine deaminase deficiency
Question 2
WRONG
Emasculation is related to
Clonal selection
Mass selection
Pure line
Hybridization
Question 3
WRONG
Which of the following terms is used to describe the component isolated from a
plant, for in vitro culturing in the specific medium?
Synthetic seeds
Embryoid
 Callus
Explant
Question 4
WRONG
Nucleic acid segment tagged with a radioactive molecule is called
plasmid
probe
clone
vector
Question 5
WRONG
Which one of the following hydrolyses internal phosphodiester bonds in a
polynucleotide chain?
Exonuclease
Protease
Lipase
Endonuclease
Question 6
WRONG
The enzyme employed for amplification of DNA during PCR is commercially obtained
from
Trichoderma reesei
Bacillus licheniformis
Streptococcus pyogenes
Thermus aquaticus
Question 7
WRONG
A regulatory body working under MoEF for the release of transgenic crops is
NBPGR
GEAC
NSC
NIPGR
Question 8
WRONG
Cry1 endotoxins obtained from Bacillus Thuringiensis are effective against:
Flies
Mosquitoes
Boll worms
Nematodes
Question 9
WRONG
In genetic engineering, a DNA segment (gene) of interest, is transferred to the host
cell through a vector. Consider the following four agents (A-D) in this regard and
select the correct option about which one or more of these can be used as a
vector/vectors
Statements
(A) A bacterium
(B) Plasmid
(C) Plasmodium
(D) Bacteriophage
(A), (B) and (D) only
(A) only
(A) and (C) only
(B) and (D) only
Question 10
WRONG
Read the following four statements (A-D) about certain mistakes in two of them
(A) The first transgenic buffalo Rosie produced milk which was human alpha-
lactalbumin enriched
(B) Restriction enzymes are used in isolation of DNA from other macro-molecules
(C) Downstream processing is one of the steps of R-DNA technology
(D) Disarmed pathogen vectors are also used in transfer of R-DNA into the host
Which are the two statements having mistakes?
Statements (A) and (C)
Statements (A) and (B)
Statements (B) and (C)
Statements (C) and (D)
Question 11
WRONG
Cry II Ab and Cry I Ab produce toxins that control
Tobacco budworms and nematodes respectively
Corn borer and cotton bollworms respectively
Cotton bollworms and corn borer respectively
Nematodes and tobacco budworms respectively
Question 12
 WRONG
Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This
protein
Binds with epithelial cells of midgut of the insect
pest ultimately killing it
Does not kill the carrier bacterium which is itself
resistant to this toxin
Is activated by acid pH of the foregut of the insect
pest
Is coded by several genes including the gene cry
Question 13
WRONG
What is true about Bt toxin?
Bt protein exists as active toxin in the Bacillus
The inactive protoxin gets converted into active
form in the insect gut
The concerned Bacillus has antitoxins
The activated toxin enters the ovaries of the pest to
sterilise it and thus prevent its multiplication
Question 14
WRONG
Assertion : In plant tissue culture, somatic embryos can be induced from any plant
cell. Reason : Any viable plant cell can differentiate in to somatic embryos.
Both Assertion and Reason are true and the
Reason is the correct explanation of the Assertion.
Both Assertion and Reason are true but the Reason
is not the correct explanation of the Assertion.
Assertion is true statement but Reason is false.
Both Assertion and Reason are false statements.
Question 15
WRONG
Which one of the following is a correct statement?
The anticoagulant hirudin is being produced from
transgenic Brassica napus seeds
Somatic hybridization involves fusion of two
complete plant cells carrying desired genes
"Bt" in "Bt-cotton" indicates that it is a genetically
modified organism produced through
biotechnology
"Flavr Savr" variety of tomato has enhanced the
production of ethylene which improves its taste