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Adda Vax
Adda Vax
Quality control Recommended maximum volumes for injection of antigen/adjuvant mixtures per site of
- AddaVax is VacciGrade (preclinical grade). It is prepared under injection for laboratory animals. (Lindblad EB., 2000. Freunds Adjuvants. In: Vaccine
adjuvants: Preparation Methods and Research Protocols. Humana Press.Totowa, NJ).
strict aseptic conditions and is tested for the presence of endotoxins.
AddaVax is guaranteed sterile and its endotoxin level is <1 EU/ml Species Max. volume Injection Site
(measurement by HEK-Blue LPS Detection Kit 2). Mice, hamsters 100 ml subcutaneous (s.c.)
- Adjuvanticity of AddaVax was evaluated by assessing the levels of Mice, hamsters 50 ml intramuscular (i.m.)
total mouse IgG (mIgG) and the mIgG1 and mIgG2 isotypes after two Guinea pigs 200 ml s.c. or i.m.
subcutaneous injections of EndoFit Ovalbumin/AddaVax (1:1, v/v)
Rats 200 ml s.c. or i.m.
in mice. Results were compared to mice receiving antigen alone.
Rabbits 250 ml s.c. or i.m.
DESCRIPTION
AddaVax is a squalene-based oil-in-water nano-emulsion based on
the formulation of MF59 that has been licensed in Europe for
adjuvanted flu vaccines1. Squalene is an oil that is more readily
metabolized than the paraffin oil used in Freunds adjuvants2.
Squalene oil-in-water emulsions, such as MF59, elicit both cellular
(Th1) and humoral (Th2) immune responses2, 3. This class of adjuvants
is believed to act through a depot effect, enhancement of antigen
persistence at the injection site, recruitment and activation of antigen
presenting cells, and direct stimulation of cytokine and chemokine
production by macrophages and granulocytes1.
MF59 is a registered trademark used for adjuvants for vaccine owned by Novartis Ag. Figure 1. Anti-Ova mIgG levels at 15, 30 and 45 days after the initial immunization
in different groups. Mice were immunized s.c at 0, 2 and 3 weeks with 1 g of EndoFit
1. Mbow ML. et al., 2010. New adjuvants for human vaccines. Curr Opin Immunol. Ovalbumin alone or 1 g of EndoFit Ovalbumin/AddaVax (1:1, v/v) in a final volume
22(3):411-6. 2. Calabro, S. et al., 2013. The adjuvant effect of MF59 is due to the oil-in- of 100 l. Serum anti-OVA mIgG was monitored by ELISA (coated with ovalbumin
water emulsion formulation, none of the individual components induce a comparable at 10 g/ml in PBS).
adjuvant effect. Vaccine 31:33639. 3. Ott G. et al., 1995. MF59. Design and evaluation
of a safe and potent adjuvant for human vaccines. Pharm Biotechnol 6:277-96.
TECHNICAL SUPPORT
InvivoGen USA (TollFree): 888-457-5873
InvivoGen USA (International): +1 (858) 457-5873
InvivoGen Europe: +33 (0) 5-62-71-69-39
InvivoGen Hong Kong: +852 3-622-34-80
www.invivogen.com
E-mail: [email protected]
RELATED PRODUCTS
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OVA Antigens
EndoFit Ovalbumin (endotoxin-free) For in vivo use; endotoxin level <1 EU/mg vac-pova
Ovalbumin For detection; Western, ELISA vac-stova
Ova 257-264 For detection; ELISPOT vac-sin
Ova 323-339 For detection; ELISPOT vac-isq
TECHNICAL SUPPORT
InvivoGen USA (TollFree): 888-457-5873
InvivoGen USA (International): +1 (858) 457-5873
InvivoGen Europe: +33 (0) 5-62-71-69-39 www.invivogen.com
InvivoGen Hong Kong: +852 3-622-34-80
E-mail: [email protected]